Rroxscaffold_3G00222210

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000952
Physical Location & Seq
Reverse (-)
5204675 .. 5206908
2234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222210.1

Sequence Viewer

Length: 1146 bp
ATGGCTAAAAGTGCATGGGAGATTTTGGAACAAGAGTGCAAGATTCGGCCAGGATACGATGAGAACAATGCTGATCACCTCAAGCTTTTTGAATCGTTCACCAAATCTGTGAGCAGTGGTGACTGGGATGAGGCAAAGAAGTGTCTCGGAGAAGTTGATGTCCGCAGCGTAGCAAGAGCAATAGATCCAAGAGACGAGCTTAGAGACACGGCTCTTCATGTAGCAGCCAGGGAAGGGCACGTGCATATCGTAAAAGAGTTGGTACATTTAATGACAAAAGAAGATTTGAAAACGAAAAACGCTGAAGGTCACACAGCTCTTCACCTAGCAACATGGAACGGGCATGTCGACATTGTGAAAGAATTGGGGTCCGTGATGGGAGAAGTAAAGGATGAGTCTGGTTTCACGGCTCTTCACATAGCAGTCCAGGAAGGGAAGGCCGCTATTGTGAAAGAGTTGGTGGTGTTGATGAGACGAGGAGACTTGAAAATAATAAACGACGCTGGTTACACAGCTCTTCACCTAGCCGTCAATATGGGGAATATGCCTATTGTCAAAGAGCTTGTGAGGAAAGAAGAAGGTGAGAATAACATTGGCGACGACGACTTTGAACGTTATATACCGTTTACCAACTATGTGCAAAATGGCGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCTTGTGGTGCAATAACAGCAATAGATCCAAGAGAGGACTGCGAAGACACTGCTCTTCACGTAGCAGCCGGGGGAGGGCATTTGGATGTTACCAAAGGATTGGTGTCGTCGCTGAAAATGAGACAAGAAGATTTGGACTTGAAAACCGCTGAAAATTTCACAACTTTTGGTTCTGGTATAGCTTTAGGGGTCAGTGAAGAATTCCTCATGGAAAGGGCCAAATACATGGTTGAACAGTACGAGAAAACACTTGGCAGCCTTTTGGAGATACAAAACGCTGAAGGGTCCACAGCTCTTCACCTAGCAGTACTGGAGGGGTTTGGGGATGTTGTGAAAGAGTTGGTGCCAGTAATGAGAAAAGAAGGGCATGTGGATATTGCAAAGGAGTTAGTGCAATCGATGAGACAAGAAGGTTTGGAAATAAAAACAAAAACGGTTCCAATGCTCTTCACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

381

Amino Acids

42.26

Weight (kDa)

5.02

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 64 - 122 3.9e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 72 - 120 1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 129 - 191 5.6e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 130 - 176 7.1e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1033
AccI GTMKAC 1 cut(s) 348
AciI CCGC 3 cut(s) 163, 441, 837
AclI AACGTT 1 cut(s) 613
AclWI GGATC 2 cut(s) 179, 710
AcoI YGGCCR 1 cut(s) 47
AcsI RAATTY 2 cut(s) 844, 890
AcuI CTGAAG 2 cut(s) 324, 990
AcvI CACGTG 1 cut(s) 241
AfaI GTAC 3 cut(s) 264, 929, 999
AfiI CCNNNNNNNGG 2 cut(s) 234, 765
AgsI TTSAA 6 cut(s) 92, 289, 487, 611, 832, 923
AjnI CCWGG 3 cut(s) 49, 227, 426
AluBI AGCT 7 cut(s) 85, 199, 317, 515, 562, 872, 983
AluI AGCT 7 cut(s) 85, 199, 317, 515, 562, 872, 983
Alw26I GTCTC 7 cut(s) 149, 186, 198, 466, 474, 805, 1087
AlwI GGATC 2 cut(s) 179, 710
AoxI GGCC 3 cut(s) 47, 438, 906
ApeKI GCWGC 4 cut(s) 165, 224, 755, 945
ApoI RAATTY 2 cut(s) 844, 890
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
AspS9I GGNCC 3 cut(s) 369, 906, 975
AsuC2I CCSGG 1 cut(s) 760
AsuHPI GGTGA 8 cut(s) 68, 91, 131, 314, 512, 593, 980, 1132
AvaII GGWCC 2 cut(s) 369, 975
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 1033
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 741
BbvI GCAGC 4 cut(s) 177, 236, 767, 957
BccI CCATC 1 cut(s) 370
BceAI ACGGC 3 cut(s) 225, 423, 512
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 3 cut(s) 51, 229, 428
BciVI GTATCC 1 cut(s) 47
BclI TGATCA 1 cut(s) 73
BcnI CCSGG 1 cut(s) 760
BcoDI GTCTC 7 cut(s) 149, 186, 198, 466, 474, 805, 1087
BfaI CTAG 5 cut(s) 326, 524, 683, 992, 1144
BfuI GTATCC 1 cut(s) 47
BisI GCNGC 5 cut(s) 166, 225, 441, 756, 946
BlsI GCNGC 5 cut(s) 167, 226, 442, 757, 947
BmcAI AGTACT 1 cut(s) 999
Bme1390I CCNGG 4 cut(s) 51, 229, 428, 760
Bme18I GGWCC 2 cut(s) 369, 975
BmgT120I GGNCC 3 cut(s) 369, 906, 975
BmiI GGNNCC 4 cut(s) 370, 976, 1035, 1128
BmrFI CCNGG 4 cut(s) 51, 229, 428, 760
BmrI ACTGGG 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 741
BpmI CTGGAG 1 cut(s) 1022
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 760
Bsa29I ATCGAT 1 cut(s) 1088
BsaAI YACGTR 2 cut(s) 241, 751
BsaJI CCNNGG 2 cut(s) 228, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 765
Bse1I ACTGG 3 cut(s) 128, 1005, 1037
BseBI CCWGG 3 cut(s) 51, 229, 428
BseCI ATCGAT 1 cut(s) 1088
BseDI CCNNGG 2 cut(s) 228, 759
BseGI GGATG 4 cut(s) 133, 397, 781, 1021
BseLI CCNNNNNNNGG 2 cut(s) 234, 765
BseNI ACTGG 3 cut(s) 128, 1005, 1037
BseRI GAGGAG 1 cut(s) 492
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 4 cut(s) 177, 236, 767, 957
BshFI GGCC 3 cut(s) 49, 440, 908
BshNI GGYRCC 1 cut(s) 1033
BshVI ATCGAT 1 cut(s) 1088
BsiSI CCGG 1 cut(s) 759
BslI CCNNNNNNNGG 2 cut(s) 234, 765
BsmAI GTCTC 7 cut(s) 149, 186, 198, 466, 474, 805, 1087
BsmBI CGTCTC 2 cut(s) 186, 466
BsnI GGCC 3 cut(s) 49, 440, 908
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 3 cut(s) 73, 184, 715
BspACI CCGC 3 cut(s) 163, 441, 837
BspANI GGCC 3 cut(s) 49, 440, 908
BspDI ATCGAT 1 cut(s) 1088
BspLI GGNNCC 4 cut(s) 370, 976, 1035, 1128
BspPI GGATC 2 cut(s) 179, 710
BspQI GCTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
BspT107I GGYRCC 1 cut(s) 1033
BsrI ACTGG 3 cut(s) 128, 1005, 1037
BssECI CCNNGG 2 cut(s) 228, 759
BssMI GATC 3 cut(s) 73, 184, 715
Bst2UI CCWGG 3 cut(s) 51, 229, 428
Bst4CI ACNGT 3 cut(s) 624, 927, 1126
Bst6I CTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
BstBAI YACGTR 2 cut(s) 241, 751
BstDEI CTNAG 2 cut(s) 200, 674
BstF5I GGATG 4 cut(s) 133, 397, 781, 1021
BstKTI GATC 3 cut(s) 76, 187, 718
BstMAI GTCTC 7 cut(s) 149, 186, 198, 466, 474, 805, 1087
BstMBI GATC 3 cut(s) 73, 184, 715
BstMWI GCNNNNNNNGC 2 cut(s) 11, 707
BstNI CCWGG 3 cut(s) 51, 229, 428
BstNSI RCATGY 2 cut(s) 347, 1061
BstSCI CCNGG 4 cut(s) 49, 227, 426, 758
BstSLI GKGCMC 1 cut(s) 240
BstV1I GCAGC 4 cut(s) 177, 236, 767, 957
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 184, 715
BstXI CCANNNNNNTGG 2 cut(s) 790, 916
BstYI RGATCY 2 cut(s) 184, 715
Bsu15I ATCGAT 1 cut(s) 1088
BsuI GTATCC 1 cut(s) 47
BsuRI GGCC 3 cut(s) 49, 440, 908
BsuTUI ATCGAT 1 cut(s) 1088
BtsCI GGATG 4 cut(s) 133, 397, 781, 1021
BtsI GCAGTG 2 cut(s) 121, 738
BtsIMutI CAGTG 3 cut(s) 121, 738, 889
Cfr13I GGNCC 3 cut(s) 369, 906, 975
ClaI ATCGAT 1 cut(s) 1088
CseI GACGC 1 cut(s) 509
Csp6I GTAC 3 cut(s) 263, 928, 998
CviAII CATG 7 cut(s) 15, 218, 333, 344, 898, 916, 1058
CviQI GTAC 3 cut(s) 263, 928, 998
DdeI CTNAG 2 cut(s) 200, 674
DpnI GATC 3 cut(s) 75, 186, 717
DpnII GATC 3 cut(s) 73, 184, 715
EaeI YGGCCR 1 cut(s) 47
Eam1104I CTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
EarI CTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
Eco47I GGWCC 2 cut(s) 369, 975
Eco57I CTGAAG 2 cut(s) 324, 990
Eco72I CACGTG 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 890
EcoRII CCWGG 3 cut(s) 49, 227, 426
Esp3I CGTCTC 2 cut(s) 186, 466
FaeI CATG 7 cut(s) 18, 221, 336, 347, 901, 919, 1061
FatI CATG 7 cut(s) 14, 217, 332, 343, 897, 915, 1057
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 5 cut(s) 166, 225, 441, 756, 946
FokI GGATG 4 cut(s) 140, 404, 788, 1028
Fsp4HI GCNGC 5 cut(s) 166, 225, 441, 756, 946
FspBI CTAG 5 cut(s) 326, 524, 683, 992, 1144
GluI GCNGC 5 cut(s) 166, 225, 441, 756, 946
GsuI CTGGAG 1 cut(s) 1022
HaeIII GGCC 3 cut(s) 49, 440, 908
HapII CCGG 1 cut(s) 759
HgaI GACGC 1 cut(s) 509
Hin1II CATG 7 cut(s) 18, 221, 336, 347, 901, 919, 1061
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 83
HinfI GANTC 3 cut(s) 43, 92, 395
HpaII CCGG 1 cut(s) 759
HphI GGTGA 8 cut(s) 68, 91, 131, 314, 512, 593, 980, 1132
Hpy166II GTNNAC 4 cut(s) 99, 349, 627, 978
Hpy188I TCNGA 1 cut(s) 149
Hpy8I GTNNAC 4 cut(s) 99, 349, 627, 978
Hpy99I CGWCG 4 cut(s) 503, 602, 605, 802
HpyAV CCTTC 8 cut(s) 227, 299, 425, 430, 572, 965, 1046, 1094
HpyCH4III ACNGT 3 cut(s) 624, 927, 1126
HpyCH4IV ACGT 3 cut(s) 240, 613, 750
HpyCH4V TGCA 7 cut(s) 14, 39, 244, 640, 701, 1070, 1084
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 707
HpyF3I CTNAG 2 cut(s) 200, 674
HpySE526I ACGT 3 cut(s) 240, 613, 750
Hsp92II CATG 7 cut(s) 18, 221, 336, 347, 901, 919, 1061
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 73, 184, 715
LguI GCTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
Lsp1109I GCAGC 4 cut(s) 177, 236, 767, 957
MaeI CTAG 5 cut(s) 326, 524, 683, 992, 1144
MaeII ACGT 3 cut(s) 240, 613, 750
MaeIII GTNAC 4 cut(s) 119, 308, 506, 778
MalI GATC 3 cut(s) 75, 186, 717
MboI GATC 3 cut(s) 73, 184, 715
MflI RGATCY 2 cut(s) 184, 715
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 3 cut(s) 362, 844, 890
MlyI GAGTC 1 cut(s) 404
MnlI CCTC 8 cut(s) 89, 124, 470, 561, 718, 758, 905, 997
MseI TTAA 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 774
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 1 cut(s) 759
MspR9I CCNGG 4 cut(s) 51, 229, 428, 760
MvaI CCWGG 3 cut(s) 51, 229, 428
MwoI GCNNNNNNNGC 2 cut(s) 11, 707
NciI CCSGG 1 cut(s) 760
NdeII GATC 3 cut(s) 73, 184, 715
NlaIII CATG 7 cut(s) 18, 221, 336, 347, 901, 919, 1061
NlaIV GGNNCC 4 cut(s) 370, 976, 1035, 1128
NmuCI GTSAC 2 cut(s) 119, 308
NspI RCATGY 2 cut(s) 347, 1061
PciSI GCTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 345
PfeI GAWTC 2 cut(s) 43, 92
PfoI TCCNGGA 1 cut(s) 426
PkrI GCNGC 5 cut(s) 167, 226, 442, 757, 947
PleI GAGTC 1 cut(s) 403
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 1 cut(s) 403
Ppu21I YACGTR 2 cut(s) 241, 751
Psp1406I AACGTT 1 cut(s) 613
Psp6I CCWGG 3 cut(s) 49, 227, 426
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 3 cut(s) 49, 227, 426
PspN4I GGNNCC 4 cut(s) 370, 976, 1035, 1128
PspPI GGNCC 3 cut(s) 369, 906, 975
PsrI GAACNNNNNNTAC 2 cut(s) 603, 635
PsuI RGATCY 2 cut(s) 184, 715
RsaI GTAC 3 cut(s) 264, 929, 999
RsaNI GTAC 3 cut(s) 263, 928, 998
RseI CAYNNNNRTG 1 cut(s) 774
SalI GTCGAC 1 cut(s) 347
SapI GCTCTTC 7 cut(s) 219, 324, 417, 522, 750, 990, 1142
SaqAI TTAA 1 cut(s) 269
SatI GCNGC 5 cut(s) 166, 225, 441, 756, 946
Sau3AI GATC 3 cut(s) 73, 184, 715
Sau96I GGNCC 3 cut(s) 369, 906, 975
ScaI AGTACT 1 cut(s) 999
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 4 cut(s) 51, 229, 428, 760
SduI GDGCHC 1 cut(s) 240
SinI GGWCC 2 cut(s) 369, 975
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 3 cut(s) 362, 844, 890
SsiI CCGC 3 cut(s) 163, 441, 837
SspMI CTAG 5 cut(s) 326, 524, 683, 992, 1144
StyD4I CCNGG 4 cut(s) 49, 227, 426, 758
TaaI ACNGT 3 cut(s) 624, 927, 1126
TaiI ACGT 3 cut(s) 243, 616, 753
TaqI TCGA 2 cut(s) 348, 1088
TasI AATT 3 cut(s) 362, 844, 890
TatI WGTACW 1 cut(s) 997
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 2 cut(s) 43, 92
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TscAI CASTG 3 cut(s) 121, 745, 889
TseFI GTSAC 2 cut(s) 119, 308
TseI GCWGC 4 cut(s) 165, 224, 755, 945
Tsp45I GTSAC 2 cut(s) 119, 308
TspDTI ATGAA 1 cut(s) 206
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 3 cut(s) 121, 745, 889
VpaK11BI GGWCC 2 cut(s) 369, 975
XapI RAATTY 2 cut(s) 844, 890
XceI RCATGY 2 cut(s) 347, 1061
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 5 cut(s) 326, 524, 683, 992, 1144
ZrmI AGTACT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.