Rw0G003450

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00118
OG0000952
Physical Location & Seq
Reverse (-)
52812 .. 55169
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G003450.1

Sequence Viewer

Length: 1845 bp
ATGGCAAGTACTCCAGTTGGCTCTAATGCACTTGTTCTGGAAGTTCTTGACCGTAGTAACTATGAAGATTGGAGATCGCGATTGAAAACTTATTTGTTGGCTGAAGATCTTTGGGAGGCTGTCGAAGCAATCGTGAAACCTCCAAAACGTGAAGATGATCAAACTGATTATAAGGCCTGGACCAAAAAGAATGCCAAGGCTTTGTACGCAATCCAAAATACATGCGGGAGAGAGCTGTTCTCTTTTATAAGGGAAATTGAGACGGCCAAAAGTGCCTGGGAGGCTTTGGAACAAGAGTGCAAAGTTATGCAAGGATATGATGAGAACAATGCCGACCACTTAGTGCGCTATAAATCGTTCATAGAATCAGTGAGCAGTGGTGACTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATGTCCGTAGTGCATTAGTAGTAAAAACAGTATACAGATATGGCGGAGAAATAGCTCTTCATGTAGCAGCAGAGGAAGGGCATGCACATATCGTGAAAGAGTTGGTGCATTTGATGACACCAGAAGATTTGAAAACAAAAACAGATTTTGGTCACACAGCTCTTCATCTAGCAGCATACAAGGGACATCTACATATTGTGAAAGAGTTGCCGGTGCTGTTGATGGGAGAAGTAAAAGACAAGGACGGTGATACAGCTCTTCATATAGCAGTCCAGGAAGGGAATGTCGATATTGTGAAAGAGTTGGTGGTGTTGATGAGACAAGAAGACGTGAAAATAAAAAACGATGCTGGTTACATAGCTCTGCACCTAGCAGTCAATAAGGGGAATGTGCCTATGGTCAAAGAGTTCGTGAGAAAAGAAAAAGGTGAGAATAATATTGGCGAGGACGACTTTGAGCGTTATATACCGTTTACCACCTATGTGCAAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCATGTAGTGCAGTAACAGCAGTAGATCCAAGAGACGACTACGGAGACACTGCTCTTCACATAGCAGCCCAGGAAGGGCATGTGGATGTTATCAAAGGATTGGTGTTGTTGCTGAAAATGAGACAAGAGGATTTGGAATTGAAAACCGCTGAAGATTTCACAACTTTTGGTTCTGGTATAACTTTGGGGGTCAGTGAAGAATTCCTCATGGAAAGGGCAAAATACATGGTTGAACAGTACGAGAAAACACTTGGCCATCTTTTGGAGATACAAGATGCTAAAGGTTCCACAGCTCTTCGCCGAGCAGTACTGCAGGGGAATGTGGATATTGTGAAAGAGTTAGCGCCTATGATGAGACAAGAAGGTTTAGAACTAAAAAGCGTCAGTGGTTCCAATGCTCTTCAACAAGCAGTGTTGAAGGGAAATGTAGATATGGTGAAAGAGTTGGTGCCATTCATGAGAAAAGAAGGTTTAGAAATAAAATACGATGATGGAGACAGAGCTCTTAACATTCTTAACATTGCAATCTTAGAAGGGCAAGTGGATATTGCGAAGGAGTTGGTGCAGTCGATGAGACCTAAGGCTTTGGAAGAAAAAGACGGTCGAGGTTTTACAGCTTTAAATCGGGCTATCCAATGTTTCAAAGATGGAGATGTTCACGAAATTGCTAAATACATGGCTGAAAAGAATGATAAAGTATTTGGCATACCCTCCGCTCCCGACGATGGCATTCCAGTTGTCATTGCTCTTCGTTGGAGCAAATGGGAATCGGCTCGTTATATCTATTCTGTCACCCCACTTGAAGCTCTCGAGGGACCCAATGGCAGTTCACTTATTTGCTTATGTCTCTATGCTAGCCACACGCTTGGTAAGACTACTTTCAGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

614

Amino Acids

68.75

Weight (kDa)

5.21

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 2.1e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 2e-10 gag-polypeptide of LTR copia-type
Ank_2 PF12796 155 - 209 4.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 159 - 252 1.5e-17 Ankyrin repeats (3 copies)
Ank_2 PF12796 217 - 280 4.6e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 219 - 264 5.7e-06 Ankyrin repeats (many copies)
Ank PF00023 221 - 253 1e-05 Ankyrin repeat
Ank_2 PF12796 261 - 353 5.1e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 405 - 468 4.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 248
AccB1I GGYRCC 1 cut(s) 1402
AccB7I CCANNNNNTGG 1 cut(s) 1216
AccBSI CCGCTC 1 cut(s) 1670
AccI GTMKAC 1 cut(s) 450
AccII CGCG 1 cut(s) 79
AciI CCGC 4 cut(s) 225, 462, 1101, 1668
AclWI GGATC 1 cut(s) 974
AcoI YGGCCR 2 cut(s) 264, 1207
AcsI RAATTY 1 cut(s) 1154
AcuI CTGAAG 2 cut(s) 123, 1125
AdeI CACNNNGTG 1 cut(s) 343
AfaI GTAC 4 cut(s) 10, 206, 1193, 1263
AfiI CCNNNNNNNGG 3 cut(s) 1029, 1216, 1679
AgsI TTSAA 8 cut(s) 85, 550, 1096, 1187, 1358, 1372, 1597, 1757
AjiI CACGTC 1 cut(s) 748
AjnI CCWGG 4 cut(s) 176, 275, 690, 1023
AleI CACNNNNGTG 1 cut(s) 899
AluBI AGCT 9 cut(s) 235, 473, 578, 674, 779, 1247, 1457, 1571, 1760
AluI AGCT 9 cut(s) 235, 473, 578, 674, 779, 1247, 1457, 1571, 1760
Alw21I GWGCWC 1 cut(s) 1459
Alw26I GTCTC 9 cut(s) 254, 730, 981, 993, 1069, 1303, 1443, 1522, 1805
AlwI GGATC 1 cut(s) 974
Ama87I CYCGRG 1 cut(s) 1763
AoxI GGCC 3 cut(s) 174, 264, 1207
ApeKI GCWGC 3 cut(s) 485, 590, 1019
ApoI RAATTY 1 cut(s) 1154
AspLEI GCGC 2 cut(s) 348, 1300
AspS9I GGNCC 2 cut(s) 180, 1769
AsuHPI GGTGA 6 cut(s) 392, 677, 857, 923, 1402, 1738
AsuNHI GCTAGC 1 cut(s) 1808
AvaI CYCGRG 1 cut(s) 1763
AvaII GGWCC 2 cut(s) 180, 1769
AxyI CCTNAGG 1 cut(s) 1533
BalI TGGCCA 1 cut(s) 1209
BanI GGYRCC 1 cut(s) 1402
BanII GRGCYC 1 cut(s) 1459
BbsI GAAGAC 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 1459
BbvI GCAGC 3 cut(s) 497, 602, 1031
BccI CCATC 5 cut(s) 634, 1218, 1439, 1595, 1673
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 4 cut(s) 178, 277, 692, 1025
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 9 cut(s) 254, 730, 981, 993, 1069, 1303, 1443, 1522, 1805
BfaI CTAG 4 cut(s) 587, 788, 947, 1809
BfmI CTRYAG 1 cut(s) 1265
BfoI RGCGCY 1 cut(s) 1301
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 106
BisI GCNGC 3 cut(s) 486, 591, 1020
BlsI GCNGC 3 cut(s) 487, 592, 1021
BmcAI AGTACT 2 cut(s) 10, 1263
Bme1390I CCNGG 4 cut(s) 178, 277, 692, 1025
Bme18I GGWCC 2 cut(s) 180, 1769
BmeT110I CYCGRG 1 cut(s) 1763
BmgBI CACGTC 1 cut(s) 748
BmgT120I GGNCC 2 cut(s) 180, 1769
BmiI GGNNCC 5 cut(s) 1240, 1345, 1404, 1770, 1771
BmrFI CCNGG 4 cut(s) 178, 277, 692, 1025
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 754, 1219
BmtI GCTAGC 1 cut(s) 1812
BmuI ACTGGG 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 750
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BsaI GGTCTC 1 cut(s) 1522
BsaJI CCNNGG 3 cut(s) 195, 276, 1023
Bsc4I CCNNNNNNNGG 3 cut(s) 1029, 1216, 1679
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 3 cut(s) 14, 389, 1688
Bse21I CCTNAGG 1 cut(s) 1533
Bse3DI GCAATG 2 cut(s) 1473, 1695
BseBI CCWGG 4 cut(s) 178, 277, 692, 1025
BseDI CCNNGG 3 cut(s) 195, 276, 1023
BseGI GGATG 1 cut(s) 1045
BseLI CCNNNNNNNGG 3 cut(s) 1029, 1216, 1679
BseMI GCAATG 2 cut(s) 1473, 1695
BseNI ACTGG 3 cut(s) 14, 389, 1688
BseXI GCAGC 3 cut(s) 497, 602, 1031
BsgI GTGCAG 3 cut(s) 767, 984, 1538
Bsh1236I CGCG 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 1558
BshFI GGCC 3 cut(s) 176, 266, 1209
BshNI GGYRCC 1 cut(s) 1402
BsiEI CGRYCG 1 cut(s) 1558
BsiHKAI GWGCWC 1 cut(s) 1459
BsiHKCI CYCGRG 1 cut(s) 1763
BsiSI CCGG 1 cut(s) 629
BslFI GGGAC 2 cut(s) 615, 1782
BslI CCNNNNNNNGG 3 cut(s) 1029, 1216, 1679
BsmAI GTCTC 9 cut(s) 254, 730, 981, 993, 1069, 1303, 1443, 1522, 1805
BsmBI CGTCTC 2 cut(s) 254, 981
BsmFI GGGAC 2 cut(s) 615, 1782
BsmI GAATGC 2 cut(s) 196, 1683
BsnI GGCC 3 cut(s) 176, 266, 1209
Bso31I GGTCTC 1 cut(s) 1522
BsoBI CYCGRG 1 cut(s) 1763
Bsp1286I GDGCHC 1 cut(s) 1459
Bsp143I GATC 4 cut(s) 74, 106, 157, 979
Bsp68I TCGCGA 1 cut(s) 79
BspACI CCGC 4 cut(s) 225, 462, 1101, 1668
BspANI GGCC 3 cut(s) 176, 266, 1209
BspFNI CGCG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 1410
BspLI GGNNCC 5 cut(s) 1240, 1345, 1404, 1770, 1771
BspMAI CTGCAG 1 cut(s) 1269
BspOI GCTAGC 1 cut(s) 1812
BspPI GGATC 1 cut(s) 974
BspQI GCTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
BspT107I GGYRCC 1 cut(s) 1402
BspTNI GGTCTC 1 cut(s) 1522
BsrBI CCGCTC 1 cut(s) 1670
BsrDI GCAATG 2 cut(s) 1473, 1695
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 3 cut(s) 14, 389, 1688
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 3 cut(s) 195, 276, 1023
BssMI GATC 4 cut(s) 74, 106, 157, 979
BssNAI GTATAC 1 cut(s) 451
BssT1I CCWWGG 1 cut(s) 195
Bst1107I GTATAC 1 cut(s) 451
Bst2UI CCWGG 4 cut(s) 178, 277, 692, 1025
Bst4CI ACNGT 6 cut(s) 53, 448, 665, 888, 1191, 1556
Bst6I CTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
BstC8I GCNNGC 2 cut(s) 501, 1810
BstDEI CTNAG 4 cut(s) 340, 938, 1483, 1533
BstF5I GGATG 1 cut(s) 1045
BstFNI CGCG 1 cut(s) 79
BstH2I RGCGCY 1 cut(s) 1301
BstHHI GCGC 2 cut(s) 348, 1300
BstKTI GATC 4 cut(s) 77, 109, 160, 982
BstMAI GTCTC 9 cut(s) 254, 730, 981, 993, 1069, 1303, 1443, 1522, 1805
BstMBI GATC 4 cut(s) 74, 106, 157, 979
BstMCI CGRYCG 1 cut(s) 1558
BstMWI GCNNNNNNNGC 5 cut(s) 125, 206, 272, 281, 971
BstNI CCWGG 4 cut(s) 178, 277, 692, 1025
BstNSI RCATGY 3 cut(s) 225, 503, 1037
BstSCI CCNGG 4 cut(s) 176, 275, 690, 1023
BstSFI CTRYAG 1 cut(s) 1265
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 3 cut(s) 497, 602, 1031
BstV2I GAAGAC 1 cut(s) 750
BstX2I RGATCY 2 cut(s) 106, 979
BstXI CCANNNNNNTGG 1 cut(s) 1820
BstYI RGATCY 2 cut(s) 106, 979
BstZ17I GTATAC 1 cut(s) 451
Bsu36I CCTNAGG 1 cut(s) 1533
BsuRI GGCC 3 cut(s) 176, 266, 1209
BtrI CACGTC 1 cut(s) 748
BtsCI GGATG 1 cut(s) 1045
BtsI GCAGTG 3 cut(s) 382, 1002, 1371
BtsIMutI CAGTG 6 cut(s) 375, 382, 1002, 1153, 1345, 1371
BtuMI TCGCGA 1 cut(s) 79
Cac8I GCNNGC 2 cut(s) 501, 1810
CciI TCATGA 1 cut(s) 1410
CfoI GCGC 2 cut(s) 348, 1300
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 180, 1769
CseI GACGC 1 cut(s) 1324
Csp6I GTAC 4 cut(s) 9, 205, 1192, 1262
CviAII CATG 9 cut(s) 222, 479, 500, 957, 1034, 1162, 1180, 1411, 1630
CviQI GTAC 4 cut(s) 9, 205, 1192, 1262
DdeI CTNAG 4 cut(s) 340, 938, 1483, 1533
DpnI GATC 4 cut(s) 76, 108, 159, 981
DpnII GATC 4 cut(s) 74, 106, 157, 979
DraI TTTAAA 1 cut(s) 1575
DraIII CACNNNGTG 1 cut(s) 343
EaeI YGGCCR 2 cut(s) 264, 1207
Eam1104I CTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
EarI CTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
EciI GGCGGA 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 1457
Eco130I CCWWGG 1 cut(s) 195
Eco147I AGGCCT 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 1459
Eco31I GGTCTC 1 cut(s) 1522
Eco47I GGWCC 2 cut(s) 180, 1769
Eco53kI GAGCTC 1 cut(s) 1457
Eco57I CTGAAG 2 cut(s) 123, 1125
Eco81I CCTNAGG 1 cut(s) 1533
Eco88I CYCGRG 1 cut(s) 1763
EcoICRI GAGCTC 1 cut(s) 1457
EcoO109I RGGNCCY 1 cut(s) 1769
EcoRI GAATTC 1 cut(s) 1154
EcoRII CCWGG 4 cut(s) 176, 275, 690, 1023
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 1459
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 2 cut(s) 254, 981
FaeI CATG 9 cut(s) 225, 482, 503, 960, 1037, 1165, 1183, 1414, 1633
FaqI GGGAC 2 cut(s) 615, 1782
FatI CATG 9 cut(s) 221, 478, 499, 956, 1033, 1161, 1179, 1410, 1629
FauI CCCGC 1 cut(s) 218
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 3 cut(s) 486, 591, 1020
FokI GGATG 1 cut(s) 1052
FriOI GRGCYC 1 cut(s) 1459
Fsp4HI GCNGC 3 cut(s) 486, 591, 1020
FspBI CTAG 4 cut(s) 587, 788, 947, 1809
GlaI GCGC 2 cut(s) 347, 1299
GluI GCNGC 3 cut(s) 486, 591, 1020
HaeII RGCGCY 1 cut(s) 1301
HaeIII GGCC 3 cut(s) 176, 266, 1209
HapII CCGG 1 cut(s) 629
HgaI GACGC 1 cut(s) 1324
HhaI GCGC 2 cut(s) 348, 1300
Hin1II CATG 9 cut(s) 225, 482, 503, 960, 1037, 1165, 1183, 1414, 1633
Hin6I GCGC 2 cut(s) 346, 1298
HinP1I GCGC 2 cut(s) 346, 1298
HinfI GANTC 2 cut(s) 365, 1721
HpaII CCGG 1 cut(s) 629
HphI GGTGA 6 cut(s) 392, 677, 857, 923, 1402, 1738
Hpy166II GTNNAC 4 cut(s) 451, 891, 1612, 1784
Hpy8I GTNNAC 4 cut(s) 451, 891, 1612, 1784
Hpy99I CGWCG 1 cut(s) 1679
HpyAV CCTTC 8 cut(s) 488, 689, 1022, 1310, 1366, 1415, 1481, 1501
HpyCH4III ACNGT 6 cut(s) 53, 448, 665, 888, 1191, 1556
HpyCH4IV ACGT 2 cut(s) 148, 747
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 206, 272, 281, 971
HpyF3I CTNAG 4 cut(s) 340, 938, 1483, 1533
HpySE526I ACGT 2 cut(s) 148, 747
Hsp92II CATG 9 cut(s) 225, 482, 503, 960, 1037, 1165, 1183, 1414, 1633
HspAI GCGC 2 cut(s) 346, 1298
KflI GGGWCCC 1 cut(s) 1769
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 4 cut(s) 74, 106, 157, 979
LguI GCTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
LmnI GCTCC 2 cut(s) 1675, 1710
Lsp1109I GCAGC 3 cut(s) 497, 602, 1031
LweI GCATC 2 cut(s) 754, 1219
MaeI CTAG 4 cut(s) 587, 788, 947, 1809
MaeII ACGT 2 cut(s) 148, 747
MaeIII GTNAC 6 cut(s) 56, 380, 569, 770, 967, 1744
MalI GATC 4 cut(s) 76, 108, 159, 981
MbiI CCGCTC 1 cut(s) 1670
MboI GATC 4 cut(s) 74, 106, 157, 979
MflI RGATCY 2 cut(s) 106, 979
MhlI GDGCHC 1 cut(s) 1459
MlsI TGGCCA 1 cut(s) 1209
MluCI AATT 4 cut(s) 255, 1091, 1154, 1617
MluNI TGGCCA 1 cut(s) 1209
MmeI TCCRAC 1 cut(s) 1688
Mox20I TGGCCA 1 cut(s) 1209
MscI TGGCCA 1 cut(s) 1209
MseI TTAA 4 cut(s) 1461, 1470, 1574, 1843
MslI CAYNNNNRTG 2 cut(s) 899, 1038
Msp20I TGGCCA 1 cut(s) 1209
MspA1I CMGCKG 1 cut(s) 1103
MspI CCGG 1 cut(s) 629
MspR9I CCNGG 4 cut(s) 178, 277, 692, 1025
Mva1269I GAATGC 2 cut(s) 196, 1683
MvaI CCWGG 4 cut(s) 178, 277, 692, 1025
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 5 cut(s) 125, 206, 272, 281, 971
NdeII GATC 4 cut(s) 74, 106, 157, 979
NheI GCTAGC 1 cut(s) 1808
NlaIII CATG 9 cut(s) 225, 482, 503, 960, 1037, 1165, 1183, 1414, 1633
NlaIV GGNNCC 5 cut(s) 1240, 1345, 1404, 1770, 1771
NmeAIII GCCGAG 1 cut(s) 1280
NmuCI GTSAC 3 cut(s) 380, 569, 1744
NruI TCGCGA 1 cut(s) 79
NspI RCATGY 3 cut(s) 225, 503, 1037
OliI CACNNNNGTG 1 cut(s) 899
PaeI GCATGC 1 cut(s) 503
PaeR7I CTCGAG 1 cut(s) 1763
PagI TCATGA 1 cut(s) 1410
PceI AGGCCT 1 cut(s) 176
PciSI GCTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 2 cut(s) 196, 1683
PfeI GAWTC 2 cut(s) 365, 1721
PflMI CCANNNNNTGG 1 cut(s) 1216
PfoI TCCNGGA 1 cut(s) 690
PkrI GCNGC 3 cut(s) 487, 592, 1021
PpuMI RGGWCCY 1 cut(s) 1769
PsiI TTATAA 2 cut(s) 171, 248
Psp124BI GAGCTC 1 cut(s) 1459
Psp5II RGGWCCY 1 cut(s) 1769
Psp6I CCWGG 4 cut(s) 176, 275, 690, 1023
PspGI CCWGG 4 cut(s) 176, 275, 690, 1023
PspN4I GGNNCC 5 cut(s) 1240, 1345, 1404, 1770, 1771
PspPI GGNCC 2 cut(s) 180, 1769
PspPPI RGGWCCY 1 cut(s) 1769
PstI CTGCAG 1 cut(s) 1269
PsuI RGATCY 2 cut(s) 106, 979
RruI TCGCGA 1 cut(s) 79
RsaI GTAC 4 cut(s) 10, 206, 1193, 1263
RsaNI GTAC 4 cut(s) 9, 205, 1192, 1262
RseI CAYNNNNRTG 2 cut(s) 899, 1038
SacI GAGCTC 1 cut(s) 1459
SapI GCTCTTC 7 cut(s) 480, 585, 681, 1014, 1254, 1359, 1707
SaqAI TTAA 4 cut(s) 1461, 1470, 1574, 1843
SatI GCNGC 3 cut(s) 486, 591, 1020
Sau3AI GATC 4 cut(s) 74, 106, 157, 979
Sau96I GGNCC 2 cut(s) 180, 1769
ScaI AGTACT 2 cut(s) 10, 1263
ScrFI CCNGG 4 cut(s) 178, 277, 692, 1025
SduI GDGCHC 1 cut(s) 1459
SfaNI GCATC 2 cut(s) 754, 1219
SfcI CTRYAG 1 cut(s) 1265
Sfr274I CTCGAG 1 cut(s) 1763
SinI GGWCC 2 cut(s) 180, 1769
SlaI CTCGAG 1 cut(s) 1763
SmiMI CAYNNNNRTG 2 cut(s) 899, 1038
SmlI CTYRAG 1 cut(s) 1763
SmoI CTYRAG 1 cut(s) 1763
SphI GCATGC 1 cut(s) 503
Sse9I AATT 4 cut(s) 255, 1091, 1154, 1617
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 4 cut(s) 225, 462, 1101, 1668
SspI AATATT 1 cut(s) 856
SspMI CTAG 4 cut(s) 587, 788, 947, 1809
SstI GAGCTC 1 cut(s) 1459
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 4 cut(s) 176, 275, 690, 1023
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 6 cut(s) 53, 448, 665, 888, 1191, 1556
TaiI ACGT 2 cut(s) 151, 750
TaqI TCGA 5 cut(s) 123, 705, 1523, 1558, 1764
TasI AATT 4 cut(s) 255, 1091, 1154, 1617
TatI WGTACW 2 cut(s) 8, 1261
TfiI GAWTC 2 cut(s) 365, 1721
Tru1I TTAA 4 cut(s) 1461, 1470, 1574, 1843
Tru9I TTAA 4 cut(s) 1461, 1470, 1574, 1843
TscAI CASTG 6 cut(s) 375, 382, 1009, 1153, 1345, 1371
TseFI GTSAC 3 cut(s) 380, 569, 1744
TseI GCWGC 3 cut(s) 485, 590, 1019
Tsp45I GTSAC 3 cut(s) 380, 569, 1744
TspDTI ATGAA 6 cut(s) 78, 349, 467, 572, 668, 1399
TspGWI ACGGA 2 cut(s) 413, 1011
TspRI CASTG 6 cut(s) 375, 382, 1009, 1153, 1345, 1371
Van91I CCANNNNNTGG 1 cut(s) 1216
VpaK11BI GGWCC 2 cut(s) 180, 1769
XapI RAATTY 1 cut(s) 1154
XceI RCATGY 3 cut(s) 225, 503, 1037
XhoI CTCGAG 1 cut(s) 1763
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 4 cut(s) 587, 788, 947, 1809
ZrmI AGTACT 2 cut(s) 10, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.