Rh7AG473500

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000952
Physical Location & Seq
Forward (+)
65679036 .. 65698911
19876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG473500.1

Sequence Viewer

Length: 3264 bp
ATGGCAAGTACTCCAGTTGGCTCTAATGCACTTATTCTGGAAGTTCTTGACCGTAGTAACTATGAAGATTGGAGATCGCGATTAAAAACTTATTTGTTGGCTGAAGATCTTTGGGAGGCTGTCGAAGCAATCGTGAAACCTCCAAAACGTGAAGATGATCAAACTGATTATAAGGCCTGGACCAAAAAGAATGCCAAGGCTTTGTACGCAATCCAAAATACATGCGGGAGAGAGCTGTTCTCTTTTATAAGGAAAATTGAGACGGCCAAAGGTGCCTGGGAGGCTTTGGAACAAGAGTGCAAAGTTATGCAAGGATATGATGAGAACAATGTGGACCACTTGGGGCGCTATAAATCGTTCATAGAATCAGTGAGGAGTGGTGATTGGGATAAGGCAAAGAAGTGTCTTGGAGAAGTTGATATGCGCAGTGTAGTAGTAAGGGCTGTAGATGGAGATGGAGAAATCGCTCTTCATGTAGCAGCCAAGGAAGGGCATGTGCACATCGTGAAAGAGTTGGTGCATTTGATGACACAAGAAGATTTGAAAACGAAAACCACTCTTGGTCACACAGCTCTTCACGTAGCAGCGTGGATGGGGCACGTGCAAATTGTGAAAGAGTTACCGGTGCCGTTGATGGGAGAAGTAAAAGACAAGGATGGTGACACAGCTCTTCATATAGCAGTCCAGGAAGGGAAGGCCAATATTGTTAAAGAGTTGGTGCTGTTGATGAGACAAGACGACTTGAAAATAAAAAATGATGCTGGTTACACAGCTCTTCACCTAGCAGTCAACAAGGGGAATGTGCCTATGGTCAAAGAGTTCCTGAGAAAAGAAAAAGGTGAGAATAATATTGGCGAAGACGACTTTAAGCGTTTTATACCGTTTACCACTTATGTGAAAAATGGTGATTGGGATAAGGCAAATGAGAGTCTTAGAGAACTGGATAATCCATGCGGTGTAGTAACAACAGTAGATCCAAGAAACTACCGCGGAGACACTGCTCTTCACGTAGCAGCCCGGGAAAGGCATGTGGATGTTACCAAAGGATTGGTGTTGTTGCTGAAAAGGAGACAAGAAGATTTGGAATTGAAAACCGCTGAGGATTTCACCACTTTTGGTTCTGGTATAACTTTAAGGGTCAGTGAAGAATTTCTCATGGAAAGGGCCAAGTACATGGTTGAACAGTACGAGAAAACACTTGGCAGCTGTTTGGAGATACAAAACGCTCGTGGTTACACAGCTCTCCACTTGGCAGCAGAGAACGGGTTTGCGGATATTGTGAAAGAGTTGGTACCATTAATGAGAAAAGAAGGTTTAGAAATACAAGGCGCTAGTGGTGACACAGCTCTCCACTTGGCAGCACTGAACAACATGAATGTGGATATTGTAAAAGAGTTGGTGTCATTAATGAGAAAAGAAGGTTTAGAAATACAAGACGCTGACGGAGACACAGCTCTTAACAATGCATTGTTTAACAATGCATTGTTACGAGTGCGTGTGGATATTGCGAAGGAGTTGGTACAATCGATGAGACAAGAAGGTTTGGAAATAAAAAACGAAAAAGGTTCCAATGCTCTTCACCTAGCGGTCAAGAATGAGCTTATGCCTTTTGCTAAAGAGCTGATGGCATTGATGAGACCAGAGGCTTTGGCAGAAAATTTAAGTCGGGCTATTCAATATTTCATGGATGGAGACGTTTGCGAAATGGCTAAATACATGGCTGAAAAGAATGATAAAGTATTTGGCATACCCTCGGCTCCCAATGATGACATTCCAGTTGTCAGAGCTCTTCGTCATAATAAATGGAAATCAGGTCGCTATATGTACTCTGTCACTCCACTTGAAGCTCTTAAGGGACCCAATGGCAGTTCATTTATTTGTTTATGTCTCAACGCTAGCAACACGATTGATTTAGCGTGGGACTTGCTTCAACGTTACCCAAATTTGGCTTTCACTGTGGCTATACCACCTGAATCTACTCCTATGATGAAATTGGCTCGCATGCATTTTGCATTCTTAAGTGGAACACGACTCACACGTTGGCAGAGATGGCTTTATCGCCTTTATGATTGTATACCCGTACCAGAAGTTGCTCATCTTAACATTCATGATATCTCTATAAATATAGCCGAATCAAAAGATGATCATGACAATAAAGGGGATTTCATTTCCTCAGTTATGACTTTTTTTAGAAGATTTGCCAAAGGTGTCTATAAATTTGGCGGAATCCGTCATATATATTTAATGAAATCGACACATAAATCGATCTTTCAATTTCTACATTGCATGGGTGAAGCGACAAGAATTAATTATCTCACAGCAGATCAACTCGACATAGTGAAAAAATCAATCTTCAATGCTATAGAACAAGGGCATCATGAGTTTGTTACTCACCTGTGTAAAGTAAATCCAGAACTACTTTGGGAGGCTTATGATGAAAGGGGGATGACAATATTTCATTATGCAATTCGATATCGTCAAGAAGAGGTTTTTAATCTTATATATGAGCTTGAGGAAAAAAGGCGAAATGACATTGGAACTCGTCTAGTGAACTGTGACAAAAGTATGCTACATTTTGCCGCCGATTTATTCTCAGTTTCATATTTTGATCATATTCAAGATGCAACTTTGCAAATTCAAAGAGAACTACAATGGTTTAAGGAGGTGGAGAGATGTGTACCCCTTGAAATGCATGAATATCGAAATCGTTCAGACGATTTGACAGCACGGGAACTATTTACCAAGAATCACAAGAGATTGATGGAGGATGCAAAAAATTCAATGAAAGAAACAGCAAATTCTTGTACACTTATAGGTGCTCTCATTGTTACAATCATGTTTGCTGCAGCTTTCACACTTCCCGGTGGAAATAATGATAACACAGGCCTTCCCTTATTACAAAATAACAGGTTGTTCAAGGTTTTTATAGTTTCAGATACTATATCACTTTTTTCTTCCACAACTGCAGTAATTACATTCTTGGGAATACTTACCTCACGTTATGCGGAACAAGATTTCCTCAAATACTTGCACACAATGATGAGGACCGGCGTATTCACCCTTTTTTTCTCTATTGCCACCATGATGATTGCATTTTCTTGTGCACTTGCCATTATGCTTGACAAGGAAGCATGGATTGTTATACCAAGTATTTTGTTGGCCAGTGTTCCATTGATATCATTCGTATGGTGGCTGTTCCCACTCCTCGTCGATATTTTCAAGTCTGCTCATGGACCTGGAATATTTGGCCAAAGAAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

1087

Amino Acids

123.7

Weight (kDa)

6.3

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 4e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 99 1e-09 gag-polypeptide of LTR copia-type
Ank_2 PF12796 147 - 207 4.6e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 215 - 280 2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 405 - 468 2e-10 Ankyrin repeats (3 copies)
PGG PF13962 926 - 1037 1.1e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 248
Acc16I TGCGCA 1 cut(s) 425
Acc65I GGTACC 1 cut(s) 1291
AccB1I GGYRCC 3 cut(s) 272, 625, 1291
AccI GTMKAC 1 cut(s) 2074
AccII CGCG 2 cut(s) 79, 990
AclI AACGTT 1 cut(s) 1933
AclWI GGATC 1 cut(s) 968
AcoI YGGCCR 3 cut(s) 264, 3159, 3247
AcsI RAATTY 7 cut(s) 1148, 1657, 1942, 2216, 2634, 2776, 2797
AcuI CTGAAG 1 cut(s) 123
AcvI CACGTG 1 cut(s) 601
AdeI CACNNNGTG 1 cut(s) 505
AfiI CCNNNNNNNGG 4 cut(s) 489, 635, 1023, 1945
AflII CTTAAG 2 cut(s) 1850, 2017
AflIII ACRYGT 1 cut(s) 2036
AgeI ACCGGT 1 cut(s) 622
AjnI CCWGG 4 cut(s) 176, 275, 684, 3235
AleI CACNNNNGTG 1 cut(s) 893
AloI GAACNNNNNNTCC 2 cut(s) 3000, 3032
Alw21I GWGCWC 4 cut(s) 501, 1789, 2821, 3106
Alw26I GTCTC 9 cut(s) 254, 724, 987, 1063, 1440, 1525, 1630, 1686, 1892
Alw44I GTGCAC 2 cut(s) 497, 3102
AlwI GGATC 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 1017
AoxI GGCC 7 cut(s) 174, 264, 696, 1164, 2884, 3159, 3247
ApaLI GTGCAC 2 cut(s) 497, 3102
ApeKI GCWGC 8 cut(s) 479, 584, 1013, 1203, 1253, 1358, 2843, 2846
ApoI RAATTY 7 cut(s) 1148, 1657, 1942, 2216, 2634, 2776, 2797
AseI ATTAAT 3 cut(s) 1298, 1406, 2307
AsiGI ACCGGT 1 cut(s) 622
Asp700I GAANNNNTTC 2 cut(s) 1149, 2707
Asp718I GGTACC 1 cut(s) 1291
AspLEI GCGC 3 cut(s) 348, 426, 1331
AspS9I GGNCC 6 cut(s) 180, 334, 1164, 1856, 3045, 3233
AsuC2I CCSGG 3 cut(s) 1018, 1019, 2862
AsuNHI GCTAGC 1 cut(s) 1895
AvaI CYCGRG 1 cut(s) 1017
AvaII GGWCC 5 cut(s) 180, 334, 1856, 3045, 3233
BaeGI GKGCMC 3 cut(s) 501, 600, 3106
BalI TGGCCA 2 cut(s) 3161, 3249
BanI GGYRCC 3 cut(s) 272, 625, 1291
BanII GRGCYC 1 cut(s) 1789
BauI CACGAG 1 cut(s) 1227
BbrPI CACGTG 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 4 cut(s) 501, 1789, 2821, 3106
BbvCI CCTCAGC 1 cut(s) 1098
BbvI GCAGC 8 cut(s) 491, 596, 1025, 1215, 1265, 1370, 2830, 2858
BccI CCATC 9 cut(s) 443, 449, 586, 628, 650, 1618, 1682, 2043, 2755
BceAI ACGGC 2 cut(s) 279, 613
BcgI CGANNNNNNTGC 2 cut(s) 2681, 2715
BciT130I CCWGG 4 cut(s) 178, 277, 686, 3237
BclI TGATCA 3 cut(s) 157, 2143, 2608
BcnI CCSGG 3 cut(s) 1018, 1019, 2862
BcoDI GTCTC 9 cut(s) 254, 724, 987, 1063, 1440, 1525, 1630, 1686, 1892
BfaI CTAG 5 cut(s) 782, 1332, 1583, 1896, 2546
BfmI CTRYAG 4 cut(s) 444, 2361, 2844, 2964
BfoI RGCGCY 2 cut(s) 349, 1332
BfrI CTTAAG 2 cut(s) 1850, 2017
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 106
BisI GCNGC 9 cut(s) 480, 585, 1014, 1204, 1254, 1359, 2580, 2844, 2847
BlsI GCNGC 9 cut(s) 481, 586, 1015, 1205, 1255, 1360, 2581, 2845, 2848
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 7 cut(s) 178, 277, 686, 1018, 1019, 2862, 3237
Bme18I GGWCC 5 cut(s) 180, 334, 1856, 3045, 3233
BmeT110I CYCGRG 1 cut(s) 1017
BmgT120I GGNCC 6 cut(s) 180, 334, 1164, 1856, 3045, 3233
BmiI GGNNCC 7 cut(s) 274, 627, 1293, 1567, 1758, 1857, 1858
BmrFI CCNGG 7 cut(s) 178, 277, 686, 1018, 1019, 2862, 3237
BmsI GCATC 4 cut(s) 748, 2383, 2611, 2758
BmtI GCTAGC 1 cut(s) 1899
BpiI GAAGAC 1 cut(s) 864
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu10I CCTNAGC 1 cut(s) 1098
BpuEI CTTGAG 1 cut(s) 2531
BpuMI CCSGG 3 cut(s) 1018, 1019, 2862
Bsa29I ATCGAT 2 cut(s) 1526, 2264
BsaAI YACGTR 3 cut(s) 580, 601, 1009
BsaI GGTCTC 1 cut(s) 1630
BsaJI CCNNGG 6 cut(s) 195, 276, 483, 988, 1017, 1752
BsaWI WCCGGW 1 cut(s) 622
Bsc4I CCNNNNNNNGG 4 cut(s) 489, 635, 1023, 1945
Bse118I RCCGGY 2 cut(s) 622, 3047
Bse1I ACTGG 4 cut(s) 14, 945, 1775, 3162
Bse3DI GCAATG 1 cut(s) 2281
BseBI CCWGG 4 cut(s) 178, 277, 686, 3237
BseCI ATCGAT 2 cut(s) 1526, 2264
BseDI CCNNGG 6 cut(s) 195, 276, 483, 988, 1017, 1752
BseGI GGATG 6 cut(s) 597, 661, 1039, 1693, 2451, 2773
BseLI CCNNNNNNNGG 4 cut(s) 489, 635, 1023, 1945
BseMI GCAATG 1 cut(s) 2281
BseMII CTCAG 4 cut(s) 815, 1089, 2187, 2607
BseNI ACTGG 4 cut(s) 14, 945, 1775, 3162
BseRI GAGGAG 2 cut(s) 388, 3194
BseSI GKGCMC 3 cut(s) 501, 600, 3106
BseXI GCAGC 8 cut(s) 491, 596, 1025, 1215, 1265, 1370, 2830, 2858
Bsh1236I CGCG 2 cut(s) 79, 990
BshFI GGCC 7 cut(s) 176, 266, 698, 1166, 2886, 3161, 3249
BshNI GGYRCC 3 cut(s) 272, 625, 1291
BshTI ACCGGT 1 cut(s) 622
BshVI ATCGAT 2 cut(s) 1526, 2264
BsiHKAI GWGCWC 4 cut(s) 501, 1789, 2821, 3106
BsiHKCI CYCGRG 1 cut(s) 1017
BsiSI CCGG 4 cut(s) 623, 1018, 2862, 3048
BslFI GGGAC 2 cut(s) 1869, 1934
BslI CCNNNNNNNGG 4 cut(s) 489, 635, 1023, 1945
BsmAI GTCTC 9 cut(s) 254, 724, 987, 1063, 1440, 1525, 1630, 1686, 1892
BsmBI CGTCTC 2 cut(s) 254, 1686
BsmFI GGGAC 2 cut(s) 1869, 1934
BsmI GAATGC 2 cut(s) 196, 2012
BsnI GGCC 7 cut(s) 176, 266, 698, 1166, 2886, 3161, 3249
Bso31I GGTCTC 1 cut(s) 1630
BsoBI CYCGRG 1 cut(s) 1017
Bsp1286I GDGCHC 5 cut(s) 501, 600, 1789, 2821, 3106
Bsp1407I TGTACA 1 cut(s) 2804
Bsp143I GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
Bsp68I TCGCGA 1 cut(s) 79
BspANI GGCC 7 cut(s) 176, 266, 698, 1166, 2886, 3161, 3249
BspCNI CTCAG 4 cut(s) 816, 1090, 2186, 2606
BspDI ATCGAT 2 cut(s) 1526, 2264
BspFNI CGCG 2 cut(s) 79, 990
BspHI TCATGA 3 cut(s) 2107, 2146, 2377
BspLI GGNNCC 7 cut(s) 274, 627, 1293, 1567, 1758, 1857, 1858
BspMAI CTGCAG 2 cut(s) 2848, 2968
BspOI GCTAGC 1 cut(s) 1899
BspPI GGATC 1 cut(s) 968
BspQI GCTCTTC 7 cut(s) 474, 579, 675, 780, 1008, 1581, 1794
BspT107I GGYRCC 3 cut(s) 272, 625, 1291
BspTI CTTAAG 2 cut(s) 1850, 2017
BspTNI GGTCTC 1 cut(s) 1630
BsrDI GCAATG 1 cut(s) 2281
BsrFI RCCGGY 2 cut(s) 622, 3047
BsrGI TGTACA 1 cut(s) 2804
BsrI ACTGG 4 cut(s) 14, 945, 1775, 3162
BssAI RCCGGY 2 cut(s) 622, 3047
BssECI CCNNGG 6 cut(s) 195, 276, 483, 988, 1017, 1752
BssMI GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
BssNAI GTATAC 1 cut(s) 2075
BssSI CACGAG 1 cut(s) 1227
BssT1I CCWWGG 2 cut(s) 195, 483
Bst1107I GTATAC 1 cut(s) 2075
Bst2BI CACGAG 1 cut(s) 1227
Bst2UI CCWGG 4 cut(s) 178, 277, 686, 3237
Bst4CI ACNGT 6 cut(s) 53, 882, 970, 1185, 1957, 2555
Bst6I CTCTTC 8 cut(s) 474, 579, 675, 780, 1008, 1581, 1794, 2478
BstAFI CTTAAG 2 cut(s) 1850, 2017
BstAUI TGTACA 1 cut(s) 2804
BstBAI YACGTR 3 cut(s) 580, 601, 1009
BstC8I GCNNGC 3 cut(s) 1897, 1999, 2003
BstDEI CTNAG 5 cut(s) 824, 932, 1098, 2173, 2593
BstDSI CCRYGG 1 cut(s) 988
BstF5I GGATG 6 cut(s) 597, 661, 1039, 1693, 2451, 2773
BstFNI CGCG 2 cut(s) 79, 990
BstH2I RGCGCY 2 cut(s) 349, 1332
BstHHI GCGC 3 cut(s) 348, 426, 1331
BstKTI GATC 8 cut(s) 77, 109, 160, 976, 2146, 2268, 2326, 2611
BstMAI GTCTC 9 cut(s) 254, 724, 987, 1063, 1440, 1525, 1630, 1686, 1892
BstMBI GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
BstMWI GCNNNNNNNGC 5 cut(s) 125, 206, 272, 281, 2050
BstNI CCWGG 4 cut(s) 178, 277, 686, 3237
BstNSI RCATGY 4 cut(s) 225, 497, 1031, 2005
BstSCI CCNGG 7 cut(s) 176, 275, 684, 1016, 1017, 2860, 3235
BstSFI CTRYAG 4 cut(s) 444, 2361, 2844, 2964
BstSLI GKGCMC 3 cut(s) 501, 600, 3106
BstUI CGCG 2 cut(s) 79, 990
BstV1I GCAGC 8 cut(s) 491, 596, 1025, 1215, 1265, 1370, 2830, 2858
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 2 cut(s) 106, 973
BstXI CCANNNNNNTGG 2 cut(s) 1048, 1174
BstYI RGATCY 2 cut(s) 106, 973
BstZ17I GTATAC 1 cut(s) 2075
Bsu15I ATCGAT 2 cut(s) 1526, 2264
BsuRI GGCC 7 cut(s) 176, 266, 698, 1166, 2886, 3161, 3249
BsuTUI ATCGAT 2 cut(s) 1526, 2264
BtgI CCRYGG 1 cut(s) 988
BtsCI GGATG 6 cut(s) 597, 661, 1039, 1693, 2451, 2773
BtsI GCAGTG 2 cut(s) 433, 996
BtsIMutI CAGTG 7 cut(s) 375, 433, 996, 1147, 1361, 1953, 3169
BtuMI TCGCGA 1 cut(s) 79
Cac8I GCNNGC 3 cut(s) 1897, 1999, 2003
CciI TCATGA 3 cut(s) 2107, 2146, 2377
CfoI GCGC 3 cut(s) 348, 426, 1331
Cfr10I RCCGGY 2 cut(s) 622, 3047
Cfr13I GGNCC 6 cut(s) 180, 334, 1164, 1856, 3045, 3233
Cfr42I CCGCGG 1 cut(s) 991
Cfr9I CCCGGG 1 cut(s) 1017
ClaI ATCGAT 2 cut(s) 1526, 2264
CseI GACGC 1 cut(s) 1445
CspAI ACCGGT 1 cut(s) 622
DdeI CTNAG 5 cut(s) 824, 932, 1098, 2173, 2593
DpnI GATC 8 cut(s) 76, 108, 159, 975, 2145, 2267, 2325, 2610
DpnII GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
DraIII CACNNNGTG 1 cut(s) 505
EaeI YGGCCR 3 cut(s) 264, 3159, 3247
Eam1104I CTCTTC 8 cut(s) 474, 579, 675, 780, 1008, 1581, 1794, 2478
EarI CTCTTC 8 cut(s) 474, 579, 675, 780, 1008, 1581, 1794, 2478
EciI GGCGGA 1 cut(s) 2238
Ecl136II GAGCTC 1 cut(s) 1787
Eco130I CCWWGG 2 cut(s) 195, 483
Eco147I AGGCCT 2 cut(s) 176, 2886
Eco24I GRGCYC 1 cut(s) 1789
Eco31I GGTCTC 1 cut(s) 1630
Eco32I GATATC 3 cut(s) 2113, 2474, 3177
Eco47I GGWCC 5 cut(s) 180, 334, 1856, 3045, 3233
Eco53kI GAGCTC 1 cut(s) 1787
Eco57I CTGAAG 1 cut(s) 123
Eco72I CACGTG 1 cut(s) 601
Eco88I CYCGRG 1 cut(s) 1017
EcoICRI GAGCTC 1 cut(s) 1787
EcoO109I RGGNCCY 1 cut(s) 1856
EcoRII CCWGG 4 cut(s) 176, 275, 684, 3235
EcoRV GATATC 3 cut(s) 2113, 2474, 3177
EcoT14I CCWWGG 2 cut(s) 195, 483
EcoT22I ATGCAT 4 cut(s) 1468, 1483, 2007, 2694
EcoT38I GRGCYC 1 cut(s) 1789
ErhI CCWWGG 2 cut(s) 195, 483
Esp3I CGTCTC 2 cut(s) 254, 1686
FaqI GGGAC 2 cut(s) 1869, 1934
FauI CCCGC 1 cut(s) 218
FbaI TGATCA 3 cut(s) 157, 2143, 2608
FblI GTMKAC 1 cut(s) 2074
Fnu4HI GCNGC 9 cut(s) 480, 585, 1014, 1204, 1254, 1359, 2580, 2844, 2847
FokI GGATG 6 cut(s) 604, 668, 1046, 1700, 2458, 2780
FriOI GRGCYC 1 cut(s) 1789
Fsp4HI GCNGC 9 cut(s) 480, 585, 1014, 1204, 1254, 1359, 2580, 2844, 2847
FspBI CTAG 5 cut(s) 782, 1332, 1583, 1896, 2546
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 3 cut(s) 347, 425, 1330
GluI GCNGC 9 cut(s) 480, 585, 1014, 1204, 1254, 1359, 2580, 2844, 2847
HaeII RGCGCY 2 cut(s) 349, 1332
HaeIII GGCC 7 cut(s) 176, 266, 698, 1166, 2886, 3161, 3249
HapII CCGG 4 cut(s) 623, 1018, 2862, 3048
HgaI GACGC 1 cut(s) 1445
HhaI GCGC 3 cut(s) 348, 426, 1331
Hin6I GCGC 3 cut(s) 346, 424, 1329
HinP1I GCGC 3 cut(s) 346, 424, 1329
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 7 cut(s) 365, 928, 1973, 2031, 2132, 2226, 2746
HpaII CCGG 4 cut(s) 623, 1018, 2862, 3048
Hpy166II GTNNAC 9 cut(s) 334, 499, 790, 885, 2075, 2551, 2678, 2807, 3104
Hpy188I TCNGA 3 cut(s) 1784, 2713, 2935
Hpy8I GTNNAC 9 cut(s) 334, 499, 790, 885, 2075, 2551, 2678, 2807, 3104
Hpy99I CGWCG 1 cut(s) 3212
HpyAV CCTTC 8 cut(s) 482, 683, 688, 1304, 1412, 1504, 1532, 2897
HpyCH4III ACNGT 6 cut(s) 53, 882, 970, 1185, 1957, 2555
HpyCH4IV ACGT 8 cut(s) 148, 579, 600, 1008, 1695, 1933, 2038, 2998
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 206, 272, 281, 2050
HpyF3I CTNAG 5 cut(s) 824, 932, 1098, 2173, 2593
HpySE526I ACGT 8 cut(s) 148, 579, 600, 1008, 1695, 1933, 2038, 2998
HspAI GCGC 3 cut(s) 346, 424, 1329
KflI GGGWCCC 1 cut(s) 1856
KpnI GGTACC 1 cut(s) 1295
Ksp22I TGATCA 3 cut(s) 157, 2143, 2608
KspI CCGCGG 1 cut(s) 991
Kzo9I GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
LguI GCTCTTC 7 cut(s) 474, 579, 675, 780, 1008, 1581, 1794
LmnI GCTCC 1 cut(s) 1762
Lsp1109I GCAGC 8 cut(s) 491, 596, 1025, 1215, 1265, 1370, 2830, 2858
LweI GCATC 4 cut(s) 748, 2383, 2611, 2758
MaeI CTAG 5 cut(s) 782, 1332, 1583, 1896, 2546
MaeII ACGT 8 cut(s) 148, 579, 600, 1008, 1695, 1933, 2038, 2998
MalI GATC 8 cut(s) 76, 108, 159, 975, 2145, 2267, 2325, 2610
MboI GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
MflI RGATCY 2 cut(s) 106, 973
MhlI GDGCHC 5 cut(s) 501, 600, 1789, 2821, 3106
MlsI TGGCCA 2 cut(s) 3161, 3249
MluNI TGGCCA 2 cut(s) 3161, 3249
MlyI GAGTC 2 cut(s) 937, 2025
Mox20I TGGCCA 2 cut(s) 3161, 3249
Mph1103I ATGCAT 4 cut(s) 1468, 1483, 2007, 2694
MroXI GAANNNNTTC 2 cut(s) 1149, 2707
MscI TGGCCA 2 cut(s) 3161, 3249
MslI CAYNNNNRTG 6 cut(s) 893, 1032, 1376, 3038, 3083, 3184
Msp20I TGGCCA 2 cut(s) 3161, 3249
MspA1I CMGCKG 3 cut(s) 990, 1097, 1206
MspCI CTTAAG 2 cut(s) 1850, 2017
MspI CCGG 4 cut(s) 623, 1018, 2862, 3048
MspR9I CCNGG 7 cut(s) 178, 277, 686, 1018, 1019, 2862, 3237
Mva1269I GAATGC 2 cut(s) 196, 2012
MvaI CCWGG 4 cut(s) 178, 277, 686, 3237
MvnI CGCG 2 cut(s) 79, 990
MwoI GCNNNNNNNGC 5 cut(s) 125, 206, 272, 281, 2050
NciI CCSGG 3 cut(s) 1018, 1019, 2862
NdeII GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
NheI GCTAGC 1 cut(s) 1895
NlaIV GGNNCC 7 cut(s) 274, 627, 1293, 1567, 1758, 1857, 1858
NmeAIII GCCGAG 1 cut(s) 1733
NmuCI GTSAC 5 cut(s) 563, 659, 1337, 1831, 2555
NruI TCGCGA 1 cut(s) 79
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 4 cut(s) 1468, 1483, 2007, 2694
NspI RCATGY 4 cut(s) 225, 497, 1031, 2005
OliI CACNNNNGTG 1 cut(s) 893
PaeI GCATGC 1 cut(s) 2005
PagI TCATGA 3 cut(s) 2107, 2146, 2377
PceI AGGCCT 2 cut(s) 176, 2886
PciSI GCTCTTC 7 cut(s) 474, 579, 675, 780, 1008, 1581, 1794
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 2035
PctI GAATGC 2 cut(s) 196, 2012
PdmI GAANNNNTTC 2 cut(s) 1149, 2707
PfeI GAWTC 5 cut(s) 365, 1973, 2132, 2226, 2746
PfoI TCCNGGA 1 cut(s) 684
PinAI ACCGGT 1 cut(s) 622
PkrI GCNGC 9 cut(s) 481, 586, 1015, 1205, 1255, 1360, 2581, 2845, 2848
PleI GAGTC 2 cut(s) 936, 2025
PmaCI CACGTG 1 cut(s) 601
PmlI CACGTG 1 cut(s) 601
PpsI GAGTC 2 cut(s) 936, 2025
Ppu21I YACGTR 3 cut(s) 580, 601, 1009
PpuMI RGGWCCY 1 cut(s) 1856
PshBI ATTAAT 3 cut(s) 1298, 1406, 2307
PsiI TTATAA 2 cut(s) 171, 248
Psp124BI GAGCTC 1 cut(s) 1789
Psp1406I AACGTT 1 cut(s) 1933
Psp5II RGGWCCY 1 cut(s) 1856
Psp6I CCWGG 4 cut(s) 176, 275, 684, 3235
PspCI CACGTG 1 cut(s) 601
PspGI CCWGG 4 cut(s) 176, 275, 684, 3235
PspN4I GGNNCC 7 cut(s) 274, 627, 1293, 1567, 1758, 1857, 1858
PspPI GGNCC 6 cut(s) 180, 334, 1164, 1856, 3045, 3233
PspPPI RGGWCCY 1 cut(s) 1856
PstI CTGCAG 2 cut(s) 2848, 2968
PsuI RGATCY 2 cut(s) 106, 973
PvuII CAGCTG 1 cut(s) 1206
RruI TCGCGA 1 cut(s) 79
RseI CAYNNNNRTG 6 cut(s) 893, 1032, 1376, 3038, 3083, 3184
SacI GAGCTC 1 cut(s) 1789
SacII CCGCGG 1 cut(s) 991
SapI GCTCTTC 7 cut(s) 474, 579, 675, 780, 1008, 1581, 1794
SatI GCNGC 9 cut(s) 480, 585, 1014, 1204, 1254, 1359, 2580, 2844, 2847
Sau3AI GATC 8 cut(s) 74, 106, 157, 973, 2143, 2265, 2323, 2608
Sau96I GGNCC 6 cut(s) 180, 334, 1164, 1856, 3045, 3233
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 2 cut(s) 937, 2025
ScrFI CCNGG 7 cut(s) 178, 277, 686, 1018, 1019, 2862, 3237
SduI GDGCHC 5 cut(s) 501, 600, 1789, 2821, 3106
SfaNI GCATC 4 cut(s) 748, 2383, 2611, 2758
SfcI CTRYAG 4 cut(s) 444, 2361, 2844, 2964
Sfr303I CCGCGG 1 cut(s) 991
SgrBI CCGCGG 1 cut(s) 991
SinI GGWCC 5 cut(s) 180, 334, 1856, 3045, 3233
SmaI CCCGGG 1 cut(s) 1019
SmiMI CAYNNNNRTG 6 cut(s) 893, 1032, 1376, 3038, 3083, 3184
SmlI CTYRAG 3 cut(s) 1850, 2017, 2510
SmoI CTYRAG 3 cut(s) 1850, 2017, 2510
SphI GCATGC 1 cut(s) 2005
SseBI AGGCCT 2 cut(s) 176, 2886
SspI AATATT 5 cut(s) 703, 850, 1679, 2454, 3243
SspMI CTAG 5 cut(s) 782, 1332, 1583, 1896, 2546
SstI GAGCTC 1 cut(s) 1789
StuI AGGCCT 2 cut(s) 176, 2886
StyD4I CCNGG 7 cut(s) 176, 275, 684, 1016, 1017, 2860, 3235
StyI CCWWGG 2 cut(s) 195, 483
TaaI ACNGT 6 cut(s) 53, 882, 970, 1185, 1957, 2555
TaiI ACGT 8 cut(s) 151, 582, 603, 1011, 1698, 1936, 2041, 3001
TaqI TCGA 8 cut(s) 123, 1526, 2252, 2264, 2331, 2470, 2701, 3210
TatI WGTACW 4 cut(s) 8, 1170, 1824, 2804
TauI GCSGC 1 cut(s) 2582
TfiI GAWTC 5 cut(s) 365, 1973, 2132, 2226, 2746
TscAI CASTG 7 cut(s) 375, 433, 1003, 1147, 1368, 1960, 3169
TseFI GTSAC 5 cut(s) 563, 659, 1337, 1831, 2555
TseI GCWGC 8 cut(s) 479, 584, 1013, 1203, 1253, 1358, 2843, 2846
Tsp45I GTSAC 5 cut(s) 563, 659, 1337, 1831, 2555
TspGWI ACGGA 2 cut(s) 1458, 2219
TspMI CCCGGG 1 cut(s) 1017
TspRI CASTG 7 cut(s) 375, 433, 1003, 1147, 1368, 1960, 3169
Vha464I CTTAAG 2 cut(s) 1850, 2017
VneI GTGCAC 2 cut(s) 497, 3102
VpaK11BI GGWCC 5 cut(s) 180, 334, 1856, 3045, 3233
VspI ATTAAT 3 cut(s) 1298, 1406, 2307
XapI RAATTY 7 cut(s) 1148, 1657, 1942, 2216, 2634, 2776, 2797
XceI RCATGY 4 cut(s) 225, 497, 1031, 2005
XcmI CCANNNNNNNNNTGG 1 cut(s) 2418
XmaI CCCGGG 1 cut(s) 1017
XmiI GTMKAC 1 cut(s) 2074
XmnI GAANNNNTTC 2 cut(s) 1149, 2707
XspI CTAG 5 cut(s) 782, 1332, 1583, 1896, 2546
ZrmI AGTACT 1 cut(s) 10
Zsp2I ATGCAT 4 cut(s) 1468, 1483, 2007, 2694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.