Rroxscaffold_3G00222250

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000952
Physical Location & Seq
Reverse (-)
5280425 .. 5295647
15223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222250.1

Sequence Viewer

Length: 1608 bp
ATGGCAAGTACCCTAGTTGGCTCTAATGCACTTGTTCTGGAAGTTCTTGACCGTAGTAACTATAAACATTGGAGATCACGATTGAAAACTTATTTGTTGGTTGAAGATCTCTGGGAGGCTGTCGAAGCAATCGTGGAACCTCCAAAAATTGAAGATGATCAAGCCAATTATAAGGCCTGGACCAAAAAGAACATAAAGGCTTTGTATGCAATCCAAAATTCGTGCGGGAGAGAGCTGTTCTCTATTATAAGTGAAATTGAGACGGCCAAAAGTGCCTGGGAGGCTTTGGAACAAGAGTTCAAAGTTGTACAAGGATATGATGAGAACAATGCCAACCACTTGATGTGCTATAAATCGTTCATTGAATCTGTAAGCAGTGGTGATTGGGGTAAGGCAAGGAAGTGTCTTGGAGAAGTTGATATCCGCAGTGTACTAGTAGTAAGAGCAGTAGGCACATATGGAAAAATAGCTCTTCATGTAGCATCCCAGGAAGGGCACGTGCATGTAGTGAAAGATTTGCTGCCTTTCATGACACAAGAAGATTTGAAAACAAAAACAGATTTTGGTCACACAGCTCTGCATCTAGCAGCAGGGATGGGGCATGTACATATTGTGAAAGAGTTGCCGGTGCTGTTGATGGGAGAAGTAAAAGACAATAACGGTGACACGGCTCTTCATATAGCAGTCCAGGAAGGGCACGCCGATGTTGTGAAAGAGTTGGTGTTGTTGATGAGACAAGAAGACTTGAAAATAGAAAACGATGCTGGTTACACAGCTCTTCACCTAGCAGTCAATAAGGGGAACGTGCCTATGGTCAAAGAGTTCGTTACAAAAGAAAAAGGTGAGAATAATATTGGGGAGGACGACTTTGAGCATTATATACCATTTACCAGCCATGTGACAAATGGTGATTGGGATAAGGCAAATGAGTGTCTTAGAGAACTAGATAATCCATGCAGTGCAATAACAACAGTAGATCCAAGAGACGACTACCAAGACACTGCTCTTCACGTAGCAGCCCGGGAAGGGCATTTGGATGTTATCAAAGGATTGGTGTTGTTGTTGAAAATGAGACAAGAAGATTTGGAATTGAAAACTGCCGAAGATTTCACAACTTTTGGTTATGGTATAACTTTAGGGGTCAATGAAGAATTCCTCATGGAAAGGGCCAAATACATAGTTGAACAGTACGAGAAAACACTTGGCAGCTGTTTGGAGATACAAAACGCTGAAGGTTCCACGGCTCTTCACACAGCAGTGCAGCAGGGGTATATGGATATTGCGAAGGAGTTGGTGCAATCGATGAGGCAAGAAGGTTTGGAAATAAAAAACAAAGTGGGTTCCAATGCTCTTCACAGAGCAGTATTGGAGGGAAATGTAGATATTGTGAAAGAGTTGGTGCCATTAATGAGACAAGAAGGTTTAGAAATAAAAGACGCTGATGGAGACACAGCTCTTAACATTGCAATATTACAAGGGCATGTGGATATTGCGAAGGAGTTGGTGCAATCGATGAGACAAGAAGGTTTGGAAATAAAAACAAAAACGGTTCCAATGCTCTTCACCGAGCGGTCAACAATAAGCATATGCATTTTGCTAAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

535

Amino Acids

59.79

Weight (kDa)

5.14

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 16 - 42 6.7e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 29 - 103 5.4e-11 gag-polypeptide of LTR copia-type
Ank_2 PF12796 158 - 241 3.7e-16 Ankyrin repeats (3 copies)
Ank_2 PF12796 243 - 352 7.5e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 406 - 467 2.9e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 442 - 504 3.5e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 248
AccB1I GGYRCC 1 cut(s) 1399
AccBSI CCGCTC 1 cut(s) 1570
AciI CCGC 3 cut(s) 225, 424, 1570
AclWI GGATC 1 cut(s) 971
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 2 cut(s) 217, 1151
AcuI CTGAAG 1 cut(s) 1251
AcvI CACGTG 1 cut(s) 499
AfaI GTAC 5 cut(s) 10, 309, 432, 606, 1190
AfiI CCNNNNNNNGG 2 cut(s) 492, 1026
AhlI ACTAGT 1 cut(s) 433
AjnI CCWGG 4 cut(s) 176, 275, 486, 687
AleI CACNNNNGTG 1 cut(s) 1256
AloI GAACNNNNNNTCC 2 cut(s) 281, 313
AluBI AGCT 6 cut(s) 235, 470, 575, 776, 1209, 1454
AluI AGCT 6 cut(s) 235, 470, 575, 776, 1209, 1454
Alw26I GTCTC 7 cut(s) 254, 727, 978, 1066, 1405, 1440, 1510
AlwI GGATC 1 cut(s) 971
Ama87I CYCGRG 1 cut(s) 1020
AoxI GGCC 3 cut(s) 174, 264, 1167
ApeKI GCWGC 5 cut(s) 520, 587, 1016, 1206, 1261
ApoI RAATTY 2 cut(s) 217, 1151
AseI ATTAAT 1 cut(s) 1406
AspS9I GGNCC 2 cut(s) 180, 1167
AsuC2I CCSGG 2 cut(s) 1021, 1022
AsuHPI GGTGA 6 cut(s) 392, 674, 773, 854, 920, 1555
AvaI CYCGRG 1 cut(s) 1020
AvaII GGWCC 1 cut(s) 180
BaeGI GKGCMC 2 cut(s) 498, 699
BanI GGYRCC 1 cut(s) 1399
BbrPI CACGTG 1 cut(s) 499
BbsI GAAGAC 1 cut(s) 747
BbvI GCAGC 5 cut(s) 507, 599, 1028, 1218, 1273
BccI CCATC 3 cut(s) 589, 631, 1436
BceAI ACGGC 3 cut(s) 279, 684, 1257
BciT130I CCWGG 4 cut(s) 178, 277, 488, 689
BclI TGATCA 1 cut(s) 157
BcnI CCSGG 2 cut(s) 1021, 1022
BcoDI GTCTC 7 cut(s) 254, 727, 978, 1066, 1405, 1440, 1510
BcuI ACTAGT 1 cut(s) 433
BfaI CTAG 5 cut(s) 14, 434, 584, 785, 944
BglI GCCNNNNNGGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 106
BisI GCNGC 5 cut(s) 521, 588, 1017, 1207, 1262
BlsI GCNGC 5 cut(s) 522, 589, 1018, 1208, 1263
Bme1390I CCNGG 6 cut(s) 178, 277, 488, 689, 1021, 1022
Bme18I GGWCC 1 cut(s) 180
BmeT110I CYCGRG 1 cut(s) 1020
BmgT120I GGNCC 2 cut(s) 180, 1167
BmiI GGNNCC 5 cut(s) 138, 1237, 1342, 1401, 1551
BmrFI CCNGG 6 cut(s) 178, 277, 488, 689, 1021, 1022
BmsI GCATC 3 cut(s) 491, 589, 751
BpiI GAAGAC 1 cut(s) 747
BplI GAGNNNNNCTC 2 cut(s) 224, 256
BpuMI CCSGG 2 cut(s) 1021, 1022
Bsa29I ATCGAT 2 cut(s) 1301, 1511
BsaAI YACGTR 2 cut(s) 499, 1012
BsaJI CCNNGG 4 cut(s) 276, 486, 1020, 1239
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 1026
Bse118I RCCGGY 1 cut(s) 625
Bse3DI GCAATG 1 cut(s) 1461
BseBI CCWGG 4 cut(s) 178, 277, 488, 689
BseCI ATCGAT 2 cut(s) 1301, 1511
BseDI CCNNGG 4 cut(s) 276, 486, 1020, 1239
BseGI GGATG 3 cut(s) 482, 600, 1042
BseLI CCNNNNNNNGG 2 cut(s) 492, 1026
BseMI GCAATG 1 cut(s) 1461
BseSI GKGCMC 2 cut(s) 498, 699
BseXI GCAGC 5 cut(s) 507, 599, 1028, 1218, 1273
BsgI GTGCAG 1 cut(s) 1280
BshFI GGCC 3 cut(s) 176, 266, 1169
BshNI GGYRCC 1 cut(s) 1399
BshVI ATCGAT 2 cut(s) 1301, 1511
BsiHKCI CYCGRG 1 cut(s) 1020
BsiSI CCGG 2 cut(s) 626, 1021
BslI CCNNNNNNNGG 2 cut(s) 492, 1026
BsmAI GTCTC 7 cut(s) 254, 727, 978, 1066, 1405, 1440, 1510
BsmBI CGTCTC 2 cut(s) 254, 978
BsnI GGCC 3 cut(s) 176, 266, 1169
BsoBI CYCGRG 1 cut(s) 1020
Bsp1286I GDGCHC 2 cut(s) 498, 699
Bsp1407I TGTACA 2 cut(s) 307, 604
Bsp143I GATC 4 cut(s) 74, 106, 157, 976
BspACI CCGC 3 cut(s) 225, 424, 1570
BspANI GGCC 3 cut(s) 176, 266, 1169
BspDI ATCGAT 2 cut(s) 1301, 1511
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 5 cut(s) 138, 1237, 1342, 1401, 1551
BspPI GGATC 1 cut(s) 971
BspQI GCTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
BspT107I GGYRCC 1 cut(s) 1399
BsrBI CCGCTC 1 cut(s) 1570
BsrDI GCAATG 1 cut(s) 1461
BsrFI RCCGGY 1 cut(s) 625
BsrGI TGTACA 2 cut(s) 307, 604
BssAI RCCGGY 1 cut(s) 625
BssECI CCNNGG 4 cut(s) 276, 486, 1020, 1239
BssMI GATC 4 cut(s) 74, 106, 157, 976
Bst2UI CCWGG 4 cut(s) 178, 277, 488, 689
Bst4CI ACNGT 5 cut(s) 53, 662, 973, 1188, 1549
Bst6I CTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
BstAUI TGTACA 2 cut(s) 307, 604
BstBAI YACGTR 2 cut(s) 499, 1012
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 1 cut(s) 935
BstDSI CCRYGG 1 cut(s) 1239
BstF5I GGATG 3 cut(s) 482, 600, 1042
BstKTI GATC 4 cut(s) 77, 109, 160, 979
BstMAI GTCTC 7 cut(s) 254, 727, 978, 1066, 1405, 1440, 1510
BstMBI GATC 4 cut(s) 74, 106, 157, 976
BstMWI GCNNNNNNNGC 4 cut(s) 125, 206, 272, 281
BstNI CCWGG 4 cut(s) 178, 277, 488, 689
BstNSI RCATGY 3 cut(s) 506, 605, 1484
BstSCI CCNGG 6 cut(s) 176, 275, 486, 687, 1019, 1020
BstSLI GKGCMC 2 cut(s) 498, 699
BstV1I GCAGC 5 cut(s) 507, 599, 1028, 1218, 1273
BstV2I GAAGAC 1 cut(s) 747
BstX2I RGATCY 2 cut(s) 106, 976
BstYI RGATCY 2 cut(s) 106, 976
Bsu15I ATCGAT 2 cut(s) 1301, 1511
BsuRI GGCC 3 cut(s) 176, 266, 1169
BsuTUI ATCGAT 2 cut(s) 1301, 1511
BtgI CCRYGG 1 cut(s) 1239
BtsCI GGATG 3 cut(s) 482, 600, 1042
BtsI GCAGTG 5 cut(s) 382, 433, 964, 999, 1263
BtsIMutI CAGTG 5 cut(s) 382, 433, 964, 999, 1263
Cac8I GCNNGC 1 cut(s) 699
CciI TCATGA 1 cut(s) 528
Cfr10I RCCGGY 1 cut(s) 625
Cfr13I GGNCC 2 cut(s) 180, 1167
Cfr9I CCCGGG 1 cut(s) 1020
ClaI ATCGAT 2 cut(s) 1301, 1511
CseI GACGC 1 cut(s) 1445
Csp6I GTAC 5 cut(s) 9, 308, 431, 605, 1189
CviAII CATG 8 cut(s) 476, 503, 529, 602, 896, 954, 1159, 1481
CviQI GTAC 5 cut(s) 9, 308, 431, 605, 1189
DdeI CTNAG 1 cut(s) 935
DpnI GATC 4 cut(s) 76, 108, 159, 978
DpnII GATC 4 cut(s) 74, 106, 157, 976
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
EarI CTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
Eco147I AGGCCT 1 cut(s) 176
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 1251
Eco72I CACGTG 1 cut(s) 499
Eco88I CYCGRG 1 cut(s) 1020
EcoRI GAATTC 1 cut(s) 1151
EcoRII CCWGG 4 cut(s) 176, 275, 486, 687
EcoRV GATATC 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 1592
Esp3I CGTCTC 2 cut(s) 254, 978
FaeI CATG 8 cut(s) 479, 506, 532, 605, 899, 957, 1162, 1484
FatI CATG 8 cut(s) 475, 502, 528, 601, 895, 953, 1158, 1480
FauI CCCGC 1 cut(s) 218
FauNDI CATATG 2 cut(s) 457, 1586
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 5 cut(s) 521, 588, 1017, 1207, 1262
FokI GGATG 3 cut(s) 469, 607, 1049
Fsp4HI GCNGC 5 cut(s) 521, 588, 1017, 1207, 1262
FspBI CTAG 5 cut(s) 14, 434, 584, 785, 944
GluI GCNGC 5 cut(s) 521, 588, 1017, 1207, 1262
HaeIII GGCC 3 cut(s) 176, 266, 1169
HapII CCGG 2 cut(s) 626, 1021
HgaI GACGC 1 cut(s) 1445
Hin1II CATG 8 cut(s) 479, 506, 532, 605, 899, 957, 1162, 1484
HincII GTYRAC 1 cut(s) 1575
HindII GTYRAC 1 cut(s) 1575
HinfI GANTC 1 cut(s) 365
HpaII CCGG 2 cut(s) 626, 1021
HphI GGTGA 6 cut(s) 392, 674, 773, 854, 920, 1555
Hpy166II GTNNAC 2 cut(s) 431, 1575
Hpy188III TCNNGA 4 cut(s) 38, 47, 78, 529
Hpy8I GTNNAC 2 cut(s) 431, 1575
HpyAV CCTTC 9 cut(s) 485, 686, 1019, 1226, 1279, 1307, 1412, 1489, 1517
HpyCH4III ACNGT 5 cut(s) 53, 662, 973, 1188, 1549
HpyCH4IV ACGT 3 cut(s) 498, 804, 1011
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 206, 272, 281
HpyF3I CTNAG 1 cut(s) 935
HpySE526I ACGT 3 cut(s) 498, 804, 1011
Hsp92II CATG 8 cut(s) 479, 506, 532, 605, 899, 957, 1162, 1484
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 4 cut(s) 74, 106, 157, 976
LguI GCTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
Lsp1109I GCAGC 5 cut(s) 507, 599, 1028, 1218, 1273
LweI GCATC 3 cut(s) 491, 589, 751
MaeI CTAG 5 cut(s) 14, 434, 584, 785, 944
MaeII ACGT 3 cut(s) 498, 804, 1011
MaeIII GTNAC 6 cut(s) 56, 566, 662, 767, 826, 898
MalI GATC 4 cut(s) 76, 108, 159, 978
MbiI CCGCTC 1 cut(s) 1570
MboI GATC 4 cut(s) 74, 106, 157, 976
MflI RGATCY 2 cut(s) 106, 976
MhlI GDGCHC 2 cut(s) 498, 699
MluCI AATT 6 cut(s) 147, 166, 217, 255, 1088, 1151
MnlI CCTC 7 cut(s) 109, 150, 274, 853, 1166, 1299, 1363
Mph1103I ATGCAT 1 cut(s) 1592
MseI TTAA 2 cut(s) 1406, 1458
MslI CAYNNNNRTG 4 cut(s) 501, 702, 1035, 1256
MspA1I CMGCKG 1 cut(s) 1209
MspI CCGG 2 cut(s) 626, 1021
MspR9I CCNGG 6 cut(s) 178, 277, 488, 689, 1021, 1022
MvaI CCWGG 4 cut(s) 178, 277, 488, 689
MwoI GCNNNNNNNGC 4 cut(s) 125, 206, 272, 281
NciI CCSGG 2 cut(s) 1021, 1022
NdeI CATATG 2 cut(s) 457, 1586
NdeII GATC 4 cut(s) 74, 106, 157, 976
NlaIII CATG 8 cut(s) 479, 506, 532, 605, 899, 957, 1162, 1484
NlaIV GGNNCC 5 cut(s) 138, 1237, 1342, 1401, 1551
NmuCI GTSAC 3 cut(s) 566, 662, 898
NsiI ATGCAT 1 cut(s) 1592
NspI RCATGY 3 cut(s) 506, 605, 1484
OliI CACNNNNGTG 1 cut(s) 1256
PagI TCATGA 1 cut(s) 528
PceI AGGCCT 1 cut(s) 176
PciSI GCTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PfeI GAWTC 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 687
PkrI GCNGC 5 cut(s) 522, 589, 1018, 1208, 1263
PmaCI CACGTG 1 cut(s) 499
PmlI CACGTG 1 cut(s) 499
Ppu21I YACGTR 2 cut(s) 499, 1012
PshBI ATTAAT 1 cut(s) 1406
PsiI TTATAA 2 cut(s) 171, 248
Psp6I CCWGG 4 cut(s) 176, 275, 486, 687
PspCI CACGTG 1 cut(s) 499
PspGI CCWGG 4 cut(s) 176, 275, 486, 687
PspN4I GGNNCC 5 cut(s) 138, 1237, 1342, 1401, 1551
PspPI GGNCC 2 cut(s) 180, 1167
PsuI RGATCY 2 cut(s) 106, 976
PvuII CAGCTG 1 cut(s) 1209
RsaI GTAC 5 cut(s) 10, 309, 432, 606, 1190
RsaNI GTAC 5 cut(s) 9, 308, 431, 605, 1189
RseI CAYNNNNRTG 4 cut(s) 501, 702, 1035, 1256
SapI GCTCTTC 7 cut(s) 477, 678, 783, 1011, 1251, 1356, 1565
SaqAI TTAA 2 cut(s) 1406, 1458
SatI GCNGC 5 cut(s) 521, 588, 1017, 1207, 1262
Sau3AI GATC 4 cut(s) 74, 106, 157, 976
Sau96I GGNCC 2 cut(s) 180, 1167
ScrFI CCNGG 6 cut(s) 178, 277, 488, 689, 1021, 1022
SduI GDGCHC 2 cut(s) 498, 699
SfaNI GCATC 3 cut(s) 491, 589, 751
SinI GGWCC 1 cut(s) 180
SmaI CCCGGG 1 cut(s) 1022
SmiMI CAYNNNNRTG 4 cut(s) 501, 702, 1035, 1256
SpeI ACTAGT 1 cut(s) 433
Sse9I AATT 6 cut(s) 147, 166, 217, 255, 1088, 1151
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 3 cut(s) 225, 424, 1570
SspI AATATT 2 cut(s) 853, 1470
SspMI CTAG 5 cut(s) 14, 434, 584, 785, 944
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 6 cut(s) 176, 275, 486, 687, 1019, 1020
TaaI ACNGT 5 cut(s) 53, 662, 973, 1188, 1549
TaiI ACGT 3 cut(s) 501, 807, 1014
TaqI TCGA 3 cut(s) 123, 1301, 1511
TasI AATT 6 cut(s) 147, 166, 217, 255, 1088, 1151
TatI WGTACW 3 cut(s) 307, 430, 604
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 1406, 1458
Tru9I TTAA 2 cut(s) 1406, 1458
TscAI CASTG 5 cut(s) 382, 433, 964, 1006, 1263
TseFI GTSAC 3 cut(s) 566, 662, 898
TseI GCWGC 5 cut(s) 520, 587, 1016, 1206, 1261
Tsp45I GTSAC 3 cut(s) 566, 662, 898
TspDTI ATGAA 5 cut(s) 349, 464, 517, 665, 1161
TspMI CCCGGG 1 cut(s) 1020
TspRI CASTG 5 cut(s) 382, 433, 964, 1006, 1263
VpaK11BI GGWCC 1 cut(s) 180
VspI ATTAAT 1 cut(s) 1406
XapI RAATTY 2 cut(s) 217, 1151
XceI RCATGY 3 cut(s) 506, 605, 1484
XcmI CCANNNNNNNNNTGG 1 cut(s) 902
XmaI CCCGGG 1 cut(s) 1020
XspI CTAG 5 cut(s) 14, 434, 584, 785, 944
Zsp2I ATGCAT 1 cut(s) 1592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.