Rmu_sc0004165.1_g000086

spectrin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004165.1
OG0000952
Physical Location & Seq
Forward (+)
337030 .. 338058
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004165.1_g000086.1.cds

Sequence Viewer

Length: 1029 bp
atgcaatctaaccattcatttatgttggtgcaagtgcacggcggtgctctgaataatctctcatatcattgggataaggcaaatgaatgtcttagagaactagataatccatgtgatgcagtaacagcagtagatccaagagacgactaccgagatattgctcttcacgtagcagcccgaaaagggcgtttggatgttatcaaaggattagtgttgttgctgaaaatgagacgagaagatttggaactgaaaaccgctgaagatttcacagcttttggttatggtataactttaggggtcagtgaaaaattcctcatggaaaaggccaaatacatggttgaacagtgcgagaaaacacttggcagctttttggagatacaaaacgctaaaggtttcacaggtcttcacctagcagtactaaatgggtatgtggatattgtgaaagagttggtgccattcatgagaaaagaaggttcagaaataaaagacattgatggagacacagctcttaacattgcaatcttaaaagggcatgtggatattgcgaaggagttggtgcaatcgatgagacaggaaggtttggaaataaaaaacaaaaacggttgcacggctcttcacctagcggtcaggactcagcacaggcaaatgcacattgtcaaagagttaatgacattgatgagaccagaggctttggaagaaaaagacagtaaaggttgtacagctttaagttgtgctgttgaatgtttcaatgatggagacgtttgcgaaatggctaaatacatggctgaaaagaatgttaaagtatttaccatatgctcggctccggctaatgagattccagttttcattttattggaagatgatgagattccagttgtcagagctcttcgtctaaataaatggaaatcgtatcgctatatgttttctgtcactccacttgaagctctcgagggaccccatggaagtgattttatatgcatttgtctccaggctagcaaaacgattggtaagactactttcactttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 DUF4219

Protein Analysis

342

Amino Acids

38.41

Weight (kDa)

5.86

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 451
AciI CCGC 3 cut(s) 42, 255, 623
AclWI GGATC 1 cut(s) 128
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 2 cut(s) 417, 718
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 4 cut(s) 341, 740, 748, 941
AjnI CCWGG 1 cut(s) 987
AleI CACNNNNGTG 1 cut(s) 42
AluBI AGCT 6 cut(s) 272, 366, 506, 722, 884, 944
AluI AGCT 6 cut(s) 272, 366, 506, 722, 884, 944
Alw21I GWGCWC 3 cut(s) 39, 49, 886
Alw26I GTCTC 7 cut(s) 135, 223, 492, 562, 673, 750, 989
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 1 cut(s) 128
Ama87I CYCGRG 1 cut(s) 947
AoxI GGCC 1 cut(s) 324
ApaLI GTGCAC 1 cut(s) 35
ApeKI GCWGC 2 cut(s) 173, 363
ApoI RAATTY 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 953
AsuHPI GGTGA 2 cut(s) 398, 608
AsuNHI GCTAGC 1 cut(s) 992
AvaI CYCGRG 1 cut(s) 947
AvaII GGWCC 1 cut(s) 953
BaeGI GKGCMC 1 cut(s) 39
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 3 cut(s) 39, 49, 886
BbvI GCAGC 2 cut(s) 185, 375
BccI CCATC 2 cut(s) 488, 746
BceAI ACGGC 2 cut(s) 55, 624
BciT130I CCWGG 1 cut(s) 989
BcoDI GTCTC 7 cut(s) 135, 223, 492, 562, 673, 750, 989
BfaI CTAG 4 cut(s) 101, 410, 620, 993
BisI GCNGC 2 cut(s) 174, 364
BlsI GCNGC 2 cut(s) 175, 365
BmcAI AGTACT 1 cut(s) 417
Bme1390I CCNGG 1 cut(s) 989
Bme18I GGWCC 1 cut(s) 953
BmeT110I CYCGRG 1 cut(s) 947
BmgT120I GGNCC 1 cut(s) 953
BmiI GGNNCC 4 cut(s) 453, 822, 954, 955
BmrFI CCNGG 1 cut(s) 989
BmsI GCATC 1 cut(s) 106
BmtI GCTAGC 1 cut(s) 996
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 971
Bsa29I ATCGAT 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 169
BsaI GGTCTC 1 cut(s) 673
BsaJI CCNNGG 1 cut(s) 958
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse1I ACTGG 2 cut(s) 839, 872
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 1 cut(s) 989
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 1 cut(s) 958
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 2 cut(s) 839, 872
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 2 cut(s) 185, 375
BshFI GGCC 1 cut(s) 326
BshNI GGYRCC 1 cut(s) 451
BshVI ATCGAT 1 cut(s) 563
BsiHKAI GWGCWC 3 cut(s) 39, 49, 886
BsiHKCI CYCGRG 1 cut(s) 947
BsiSI CCGG 1 cut(s) 824
BslFI GGGAC 1 cut(s) 966
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 7 cut(s) 135, 223, 492, 562, 673, 750, 989
BsmBI CGTCTC 3 cut(s) 135, 223, 750
BsmFI GGGAC 1 cut(s) 966
BsnI GGCC 1 cut(s) 326
Bso31I GGTCTC 1 cut(s) 673
BsoBI CYCGRG 1 cut(s) 947
Bsp1286I GDGCHC 3 cut(s) 39, 49, 886
Bsp1407I TGTACA 1 cut(s) 716
Bsp143I GATC 1 cut(s) 133
Bsp19I CCATGG 1 cut(s) 958
BspACI CCGC 3 cut(s) 42, 255, 623
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 646
BspDI ATCGAT 1 cut(s) 563
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 4 cut(s) 453, 822, 954, 955
BspOI GCTAGC 1 cut(s) 996
BspPI GGATC 1 cut(s) 128
BspQI GCTCTTC 3 cut(s) 168, 618, 891
BspT107I GGYRCC 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 673
BsrDI GCAATG 1 cut(s) 513
BsrGI TGTACA 1 cut(s) 716
BsrI ACTGG 2 cut(s) 839, 872
BssECI CCNNGG 1 cut(s) 958
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 958
Bst2UI CCWGG 1 cut(s) 989
Bst4CI ACNGT 3 cut(s) 345, 602, 707
Bst6I CTCTTC 3 cut(s) 168, 618, 891
BstAUI TGTACA 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 994
BstDEI CTNAG 2 cut(s) 92, 633
BstDSI CCRYGG 1 cut(s) 958
BstF5I GGATG 1 cut(s) 199
BstKTI GATC 1 cut(s) 136
BstMAI GTCTC 7 cut(s) 135, 223, 492, 562, 673, 750, 989
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 989
BstNSI RCATGY 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 987
BstSLI GKGCMC 1 cut(s) 39
BstV1I GCAGC 2 cut(s) 185, 375
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 563
BsuRI GGCC 1 cut(s) 326
BsuTUI ATCGAT 1 cut(s) 563
BtgI CCRYGG 1 cut(s) 958
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 2 cut(s) 307, 350
Cac8I GCNNGC 1 cut(s) 994
CciI TCATGA 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 953
ClaI ATCGAT 1 cut(s) 563
Csp6I GTAC 2 cut(s) 416, 717
CviAII CATG 7 cut(s) 111, 316, 334, 460, 533, 781, 959
CviQI GTAC 2 cut(s) 416, 717
DdeI CTNAG 2 cut(s) 92, 633
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Eam1104I CTCTTC 3 cut(s) 168, 618, 891
EarI CTCTTC 3 cut(s) 168, 618, 891
Ecl136II GAGCTC 1 cut(s) 884
Eco130I CCWWGG 1 cut(s) 958
Eco24I GRGCYC 1 cut(s) 886
Eco31I GGTCTC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 953
Eco53kI GAGCTC 1 cut(s) 884
Eco57I CTGAAG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 947
EcoICRI GAGCTC 1 cut(s) 884
EcoO109I RGGNCCY 1 cut(s) 953
EcoRII CCWGG 1 cut(s) 987
EcoT14I CCWWGG 1 cut(s) 958
EcoT22I ATGCAT 1 cut(s) 980
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 1 cut(s) 958
Esp3I CGTCTC 3 cut(s) 135, 223, 750
FaeI CATG 7 cut(s) 114, 319, 337, 463, 536, 784, 962
FaqI GGGAC 1 cut(s) 966
FatI CATG 7 cut(s) 110, 315, 333, 459, 532, 780, 958
FauNDI CATATG 1 cut(s) 812
Fnu4HI GCNGC 2 cut(s) 174, 364
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 2 cut(s) 174, 364
FspBI CTAG 4 cut(s) 101, 410, 620, 993
GluI GCNGC 2 cut(s) 174, 364
GsuI CTGGAG 1 cut(s) 971
HaeIII GGCC 1 cut(s) 326
HapII CCGG 1 cut(s) 824
Hin1II CATG 7 cut(s) 114, 319, 337, 463, 536, 784, 962
HinfI GANTC 3 cut(s) 631, 835, 868
HpaII CCGG 1 cut(s) 824
HphI GGTGA 2 cut(s) 398, 608
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 51, 478, 881
Hpy188III TCNNGA 3 cut(s) 460, 628, 947
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 3 cut(s) 464, 541, 569
HpyCH4III ACNGT 3 cut(s) 345, 602, 707
HpyCH4IV ACGT 2 cut(s) 168, 759
HpyCH4V TGCA 9 cut(s) 4, 31, 37, 119, 518, 559, 606, 649, 978
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 92, 633
HpySE526I ACGT 2 cut(s) 168, 759
Hsp92II CATG 7 cut(s) 114, 319, 337, 463, 536, 784, 962
KflI GGGWCCC 1 cut(s) 953
Kzo9I GATC 1 cut(s) 133
LguI GCTCTTC 3 cut(s) 168, 618, 891
LmnI GCTCC 1 cut(s) 826
Lsp1109I GCAGC 2 cut(s) 185, 375
LweI GCATC 1 cut(s) 106
MaeI CTAG 4 cut(s) 101, 410, 620, 993
MaeII ACGT 2 cut(s) 168, 759
MaeIII GTNAC 2 cut(s) 121, 928
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 8 cut(s) 155, 248, 272, 395, 605, 707, 869, 878
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 3 cut(s) 39, 49, 886
MluCI AATT 1 cut(s) 308
MlyI GAGTC 1 cut(s) 625
MnlI CCTC 3 cut(s) 323, 679, 943
Mph1103I ATGCAT 1 cut(s) 980
MseI TTAA 5 cut(s) 510, 524, 665, 725, 798
MslI CAYNNNNRTG 2 cut(s) 42, 963
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 1 cut(s) 824
MspR9I CCNGG 1 cut(s) 989
MvaI CCWGG 1 cut(s) 989
MwoI GCNNNNNNNGC 1 cut(s) 125
NcoI CCATGG 1 cut(s) 958
NdeI CATATG 1 cut(s) 812
NdeII GATC 1 cut(s) 133
NheI GCTAGC 1 cut(s) 992
NlaIII CATG 7 cut(s) 114, 319, 337, 463, 536, 784, 962
NlaIV GGNNCC 4 cut(s) 453, 822, 954, 955
NmeAIII GCCGAG 1 cut(s) 797
NmuCI GTSAC 1 cut(s) 928
NsiI ATGCAT 1 cut(s) 980
NspI RCATGY 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 42
PaeR7I CTCGAG 1 cut(s) 947
PagI TCATGA 1 cut(s) 459
PciSI GCTCTTC 3 cut(s) 168, 618, 891
PfeI GAWTC 2 cut(s) 835, 868
PkrI GCNGC 2 cut(s) 175, 365
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
Ppu21I YACGTR 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 886
Psp5II RGGWCCY 1 cut(s) 953
Psp6I CCWGG 1 cut(s) 987
PspGI CCWGG 1 cut(s) 987
PspN4I GGNNCC 4 cut(s) 453, 822, 954, 955
PspPI GGNCC 1 cut(s) 953
PspPPI RGGWCCY 1 cut(s) 953
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 2 cut(s) 417, 718
RsaNI GTAC 2 cut(s) 416, 717
RseI CAYNNNNRTG 2 cut(s) 42, 963
SacI GAGCTC 1 cut(s) 886
SapI GCTCTTC 3 cut(s) 168, 618, 891
SaqAI TTAA 5 cut(s) 510, 524, 665, 725, 798
SatI GCNGC 2 cut(s) 174, 364
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 1 cut(s) 953
ScaI AGTACT 1 cut(s) 417
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 1 cut(s) 989
SduI GDGCHC 3 cut(s) 39, 49, 886
SfaNI GCATC 1 cut(s) 106
Sfr274I CTCGAG 1 cut(s) 947
SinI GGWCC 1 cut(s) 953
SlaI CTCGAG 1 cut(s) 947
SmiMI CAYNNNNRTG 2 cut(s) 42, 963
SmlI CTYRAG 1 cut(s) 947
SmoI CTYRAG 1 cut(s) 947
Sse9I AATT 1 cut(s) 308
SsiI CCGC 3 cut(s) 42, 255, 623
SspMI CTAG 4 cut(s) 101, 410, 620, 993
SstI GAGCTC 1 cut(s) 886
StyD4I CCNGG 1 cut(s) 987
StyI CCWWGG 1 cut(s) 958
TaaI ACNGT 3 cut(s) 345, 602, 707
TaiI ACGT 2 cut(s) 171, 762
TaqI TCGA 2 cut(s) 563, 948
TasI AATT 1 cut(s) 308
TatI WGTACW 2 cut(s) 415, 716
TfiI GAWTC 2 cut(s) 835, 868
Tru1I TTAA 5 cut(s) 510, 524, 665, 725, 798
Tru9I TTAA 5 cut(s) 510, 524, 665, 725, 798
TscAI CASTG 2 cut(s) 307, 350
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 2 cut(s) 173, 363
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 4 cut(s) 6, 99, 448, 835
TspRI CASTG 2 cut(s) 307, 350
VneI GTGCAC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 953
XapI RAATTY 1 cut(s) 308
XceI RCATGY 1 cut(s) 536
XhoI CTCGAG 1 cut(s) 947
XspI CTAG 4 cut(s) 101, 410, 620, 993
ZrmI AGTACT 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.