FvH4_6g52860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001373
Physical Location & Seq
Reverse (-)
38824522 .. 38827418
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52860.t1

Sequence Viewer

Length: 1254 bp
ATGTGGGAGATTGGTAAACCCACTTTCCCGGCGACCCATCTCCCAAATTTCCCGGAAACCATGAAAAGGCAATCCCGATTATCCTCCTCGTCGAAGAAGACGACGAAGCTACCACCACCCAGCACGGCCGCTCCGCCAAAACCAGTCCCCTATTTCAACCCATTAGCCCGAGGCCCAAGAAACGAGCGGCATCACAACCAAGAACGAATCTTTCTGCACCATTCAAACCGCACAGTTCATCCCAAGATCCTATTTGTAAGAGATGATGGCGAAGTGGAGACAAAGACGACCGCTTTACCTTCCTCCGGTGCACAGCTTGAGCTCCTCGGCCAGTCCCGGGATAAGGTGTGTTTCCTGAACCATTCGGCCAACACGCTGGGCATATGGGATTTGAAGAAACAGAGAATGGAACAACAAGTCGCCGTTGACACAGGGGTACCGGCGGCCCATGGATTTGGGCATACAAGAAACGGACACTTAAAAGTCAGGTTCATAATCCCACCCATTGTTCCAACGGATCGAGTTTACACCCTGGAGATCTGCAGAGAGGACCCGGAGGAGAGGAAGCTTGTTTGGTTCAAAAGAGAGGTGATCCATCCGGTGTCTTTCCATCTGGCGCAAGGTTTTGCGAAACTGGTGTGTGAGGGAGAGGATCGATTCCTGTTTTGGAAAGGGGGCTACGCAAGTGCCACACGTATTCAGATTATAAAGTTTGATTTGGAGAGAGAATCCTTGGGGCTTGTGAGAGTCCCTCCAGATGATTCTTTAAGGCATCAAAATATGTGTATTGGCCATGGATTTCAAGGCAGGCTGGCCTTGTCTGTATATGTTCCTTCATCGAATGCAGAGCCTTTTTCGATGCTGCGTGTGTGGACTCTTGAGAGGAATGATGGAGGAAATAACATCTTTTGGCATAGGCTGCTCACCCTTCCTATGGAGGCCTCCAATGTGCTTGAGCCCTTTGTTCCTTTATCCTTCTTCTATGGAAGAGGTGATTCAGGAGTGAACTTTCTGTTCCACAGGAATAACAATGAGGTCTTTCACATTGTTCTGAGAGGTGACGAGGTTGTGAGCGCTCCGATTACTATACCAGCCTTCACCTACCGTCAAGGACCTTTTGTTGGTAGGCAGTTCCAAGCCACTCGCCTCCGTCCCAAAATATCCTTCATTCTGGAGCCTTCACTGCCCAAAGTTGAGGAGGACAAGCCGATGAGATGGGATCCCAGTCTCAGTTTTGCAGATGACACTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

418

Amino Acids

47.58

Weight (kDa)

9.3

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 707
Acc65I GGTACC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 436
AccBSI CCGCTC 2 cut(s) 131, 187
AciI CCGC 6 cut(s) 129, 134, 187, 229, 291, 443
AclWI GGATC 6 cut(s) 241, 525, 586, 660, 1214, 1227
AcoI YGGCCR 4 cut(s) 126, 328, 366, 790
AcsI RAATTY 1 cut(s) 46
AfaI GTAC 1 cut(s) 438
AfeI AGCGCT 1 cut(s) 1075
AfiI CCNNNNNNNGG 6 cut(s) 66, 305, 337, 343, 934, 1121
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 5 cut(s) 157, 225, 394, 580, 803
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 1148, 1180
AluBI AGCT 4 cut(s) 109, 316, 322, 568
AluI AGCT 4 cut(s) 109, 316, 322, 568
Alw21I GWGCWC 2 cut(s) 313, 324
Alw26I GTCTC 2 cut(s) 272, 1232
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 6 cut(s) 241, 525, 586, 660, 1214, 1227
Ama87I CYCGRG 2 cut(s) 168, 336
Aor51HI AGCGCT 1 cut(s) 1075
AoxI GGCC 8 cut(s) 126, 172, 328, 366, 444, 790, 813, 939
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 862, 919
ApoI RAATTY 1 cut(s) 46
Asp718I GGTACC 1 cut(s) 436
AspLEI GCGC 2 cut(s) 619, 1076
AspS9I GGNCC 4 cut(s) 173, 445, 550, 1112
AsuC2I CCSGG 5 cut(s) 29, 53, 337, 338, 554
AsuHPI GGTGA 5 cut(s) 601, 916, 1004, 1070, 1090
AvaI CYCGRG 2 cut(s) 168, 336
AvaII GGWCC 2 cut(s) 550, 1112
BaeGI GKGCMC 1 cut(s) 313
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BalI TGGCCA 1 cut(s) 792
BamHI GGATCC 1 cut(s) 1219
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 2 cut(s) 324, 960
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 2 cut(s) 313, 324
BbvI GCAGC 2 cut(s) 849, 906
BccI CCATC 6 cut(s) 45, 260, 603, 618, 884, 1209
BceAI ACGGC 2 cut(s) 141, 407
BciT130I CCWGG 1 cut(s) 533
BcnI CCSGG 5 cut(s) 29, 53, 337, 338, 554
BcoDI GTCTC 2 cut(s) 272, 1232
BfmI CTRYAG 1 cut(s) 541
BfoI RGCGCY 1 cut(s) 1077
BglII AGATCT 1 cut(s) 537
BisI GCNGC 5 cut(s) 129, 188, 444, 863, 920
BlsI GCNGC 5 cut(s) 130, 189, 445, 864, 921
Bme1390I CCNGG 6 cut(s) 29, 53, 337, 338, 533, 554
Bme18I GGWCC 2 cut(s) 550, 1112
BmeT110I CYCGRG 2 cut(s) 168, 336
BmgT120I GGNCC 4 cut(s) 173, 445, 550, 1112
BmiI GGNNCC 4 cut(s) 438, 552, 1176, 1221
BmrFI CCNGG 6 cut(s) 29, 53, 337, 338, 533, 554
BmrI ACTGGG 1 cut(s) 1218
BmsI GCATC 3 cut(s) 199, 781, 849
BmuI ACTGGG 1 cut(s) 1218
BpiI GAAGAC 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 736, 768
BpmI CTGGAG 3 cut(s) 554, 738, 1193
BpuEI CTTGAG 3 cut(s) 338, 899, 974
BpuMI CCSGG 5 cut(s) 29, 53, 337, 338, 554
Bsa29I ATCGAT 1 cut(s) 655
BsaAI YACGTR 1 cut(s) 695
BsaJI CCNNGG 7 cut(s) 169, 325, 336, 448, 531, 732, 793
BsaWI WCCGGW 2 cut(s) 305, 598
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 305, 337, 343, 934, 1121
Bse118I RCCGGY 1 cut(s) 439
Bse1I ACTGG 4 cut(s) 143, 331, 639, 1224
BseBI CCWGG 1 cut(s) 533
BseCI ATCGAT 1 cut(s) 655
BseDI CCNNGG 7 cut(s) 169, 325, 336, 448, 531, 732, 793
BseGI GGATG 2 cut(s) 238, 595
BseLI CCNNNNNNNGG 6 cut(s) 66, 305, 337, 343, 934, 1121
BseMII CTCAG 3 cut(s) 1043, 1239, 1243
BseNI ACTGG 4 cut(s) 143, 331, 639, 1224
BseRI GAGGAG 4 cut(s) 76, 314, 572, 1211
BseSI GKGCMC 1 cut(s) 313
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 2 cut(s) 849, 906
BseYI CCCAGC 2 cut(s) 119, 376
BsgI GTGCAG 1 cut(s) 200
Bsh1285I CGRYCG 2 cut(s) 129, 291
BshFI GGCC 8 cut(s) 128, 174, 330, 368, 446, 792, 815, 941
BshNI GGYRCC 1 cut(s) 436
BshVI ATCGAT 1 cut(s) 655
BsiEI CGRYCG 2 cut(s) 129, 291
BsiHKAI GWGCWC 2 cut(s) 313, 324
BsiHKCI CYCGRG 2 cut(s) 168, 336
BsiSI CCGG 7 cut(s) 29, 53, 306, 337, 440, 554, 599
BslFI GGGAC 4 cut(s) 131, 319, 734, 1137
BslI CCNNNNNNNGG 6 cut(s) 66, 305, 337, 343, 934, 1121
BsmAI GTCTC 2 cut(s) 272, 1232
BsmFI GGGAC 4 cut(s) 131, 319, 734, 1137
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 8 cut(s) 128, 174, 330, 368, 446, 792, 815, 941
BsoBI CYCGRG 2 cut(s) 168, 336
Bsp1286I GDGCHC 3 cut(s) 313, 324, 960
Bsp143I GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
Bsp19I CCATGG 2 cut(s) 448, 793
BspACI CCGC 6 cut(s) 129, 134, 187, 229, 291, 443
BspANI GGCC 8 cut(s) 128, 174, 330, 368, 446, 792, 815, 941
BspCNI CTCAG 3 cut(s) 1044, 1240, 1242
BspDI ATCGAT 1 cut(s) 655
BspLI GGNNCC 4 cut(s) 438, 552, 1176, 1221
BspMAI CTGCAG 1 cut(s) 545
BspPI GGATC 6 cut(s) 241, 525, 586, 660, 1214, 1227
BspT107I GGYRCC 1 cut(s) 436
BsrBI CCGCTC 2 cut(s) 131, 187
BsrFI RCCGGY 1 cut(s) 439
BsrI ACTGG 4 cut(s) 143, 331, 639, 1224
BssAI RCCGGY 1 cut(s) 439
BssECI CCNNGG 7 cut(s) 169, 325, 336, 448, 531, 732, 793
BssMI GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
BssT1I CCWWGG 3 cut(s) 448, 732, 793
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 235, 1106
Bst6I CTCTTC 1 cut(s) 982
BstAPI GCANNNNNTGC 1 cut(s) 919
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 809, 813
BstDEI CTNAG 3 cut(s) 1052, 1229, 1248
BstDSI CCRYGG 2 cut(s) 448, 793
BstF5I GGATG 2 cut(s) 238, 595
BstH2I RGCGCY 1 cut(s) 1077
BstHHI GCGC 2 cut(s) 619, 1076
BstKTI GATC 6 cut(s) 249, 520, 540, 594, 655, 1222
BstMAI GTCTC 2 cut(s) 272, 1232
BstMBI GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
BstMCI CGRYCG 2 cut(s) 129, 291
BstMWI GCNNNNNNNGC 2 cut(s) 919, 1183
BstNI CCWGG 1 cut(s) 533
BstSCI CCNGG 6 cut(s) 27, 51, 335, 336, 531, 552
BstSFI CTRYAG 1 cut(s) 541
BstSLI GKGCMC 1 cut(s) 313
BstV1I GCAGC 2 cut(s) 849, 906
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 3 cut(s) 246, 537, 1219
BstXI CCANNNNNNTGG 2 cut(s) 376, 455
BstYI RGATCY 3 cut(s) 246, 537, 1219
BstZI CGGCCG 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 655
BsuRI GGCC 8 cut(s) 128, 174, 330, 368, 446, 792, 815, 941
BsuTUI ATCGAT 1 cut(s) 655
BtgI CCRYGG 2 cut(s) 448, 793
BtsCI GGATG 2 cut(s) 238, 595
BtsI GCAGTG 1 cut(s) 1181
BtsIMutI CAGTG 2 cut(s) 1181, 1245
Cac8I GCNNGC 2 cut(s) 809, 813
CfoI GCGC 2 cut(s) 619, 1076
Cfr10I RCCGGY 1 cut(s) 439
Cfr13I GGNCC 4 cut(s) 173, 445, 550, 1112
Cfr9I CCCGGG 1 cut(s) 336
ClaI ATCGAT 1 cut(s) 655
Csp6I GTAC 1 cut(s) 437
CviAII CATG 3 cut(s) 61, 449, 794
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 3 cut(s) 1052, 1229, 1248
DpnI GATC 6 cut(s) 248, 519, 539, 593, 654, 1221
DpnII GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
EaeI YGGCCR 4 cut(s) 126, 328, 366, 790
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 982
EarI CTCTTC 1 cut(s) 982
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 322
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 3 cut(s) 448, 732, 793
Eco147I AGGCCT 1 cut(s) 941
Eco24I GRGCYC 2 cut(s) 324, 960
Eco47I GGWCC 2 cut(s) 550, 1112
Eco47III AGCGCT 1 cut(s) 1075
Eco52I CGGCCG 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 322
Eco88I CYCGRG 2 cut(s) 168, 336
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 550, 1112
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 3 cut(s) 448, 732, 793
EcoT38I GRGCYC 2 cut(s) 324, 960
ErhI CCWWGG 3 cut(s) 448, 732, 793
FaeI CATG 3 cut(s) 64, 452, 797
FaqI GGGAC 4 cut(s) 131, 319, 734, 1137
FatI CATG 3 cut(s) 60, 448, 793
FauNDI CATATG 1 cut(s) 383
Fnu4HI GCNGC 5 cut(s) 129, 188, 444, 863, 920
FokI GGATG 2 cut(s) 225, 582
FriOI GRGCYC 2 cut(s) 324, 960
Fsp4HI GCNGC 5 cut(s) 129, 188, 444, 863, 920
GlaI GCGC 2 cut(s) 618, 1075
GluI GCNGC 5 cut(s) 129, 188, 444, 863, 920
GsaI CCCAGC 2 cut(s) 123, 380
GsuI CTGGAG 3 cut(s) 554, 738, 1193
HaeII RGCGCY 1 cut(s) 1077
HaeIII GGCC 8 cut(s) 128, 174, 330, 368, 446, 792, 815, 941
HapII CCGG 7 cut(s) 29, 53, 306, 337, 440, 554, 599
HhaI GCGC 2 cut(s) 619, 1076
Hin1II CATG 3 cut(s) 64, 452, 797
Hin6I GCGC 2 cut(s) 617, 1074
HinP1I GCGC 2 cut(s) 617, 1074
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 7 cut(s) 207, 657, 728, 747, 761, 874, 995
HpaII CCGG 7 cut(s) 29, 53, 306, 337, 440, 554, 599
HphI GGTGA 5 cut(s) 601, 916, 1004, 1070, 1090
Hpy166II GTNNAC 6 cut(s) 17, 311, 427, 526, 873, 1006
Hpy188I TCNGA 3 cut(s) 702, 1053, 1080
Hpy188III TCNNGA 6 cut(s) 75, 355, 755, 878, 999, 1172
Hpy8I GTNNAC 6 cut(s) 17, 311, 427, 526, 873, 1006
Hpy99I CGWCG 2 cut(s) 94, 106
HpyAV CCTTC 7 cut(s) 309, 843, 938, 985, 1105, 1174, 1188
HpyCH4III ACNGT 2 cut(s) 235, 1106
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 5 cut(s) 217, 311, 543, 845, 1238
HpyF10VI GCNNNNNNNGC 2 cut(s) 919, 1183
HpyF3I CTNAG 3 cut(s) 1052, 1229, 1248
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 3 cut(s) 64, 452, 797
HspAI GCGC 2 cut(s) 617, 1074
KpnI GGTACC 1 cut(s) 440
Kzo9I GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
LmnI GCTCC 4 cut(s) 136, 327, 1081, 1174
Lsp1109I GCAGC 2 cut(s) 849, 906
LweI GCATC 3 cut(s) 199, 781, 849
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 1 cut(s) 1058
MalI GATC 6 cut(s) 248, 519, 539, 593, 654, 1221
MbiI CCGCTC 2 cut(s) 131, 187
MboI GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
MboII GAAGA 5 cut(s) 106, 109, 406, 970, 999
MflI RGATCY 3 cut(s) 246, 537, 1219
MhlI GDGCHC 3 cut(s) 313, 324, 960
MlsI TGGCCA 1 cut(s) 792
MluCI AATT 1 cut(s) 46
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 2 cut(s) 756, 868
MmeI TCCRAC 1 cut(s) 536
Mox20I TGGCCA 1 cut(s) 792
MscI TGGCCA 1 cut(s) 792
MseI TTAA 2 cut(s) 479, 767
Msp20I TGGCCA 1 cut(s) 792
MspI CCGG 7 cut(s) 29, 53, 306, 337, 440, 554, 599
MspR9I CCNGG 6 cut(s) 29, 53, 337, 338, 533, 554
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 1 cut(s) 533
MwoI GCNNNNNNNGC 2 cut(s) 919, 1183
NciI CCSGG 5 cut(s) 29, 53, 337, 338, 554
NcoI CCATGG 2 cut(s) 448, 793
NdeI CATATG 1 cut(s) 383
NdeII GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
NlaIII CATG 3 cut(s) 64, 452, 797
NlaIV GGNNCC 4 cut(s) 438, 552, 1176, 1221
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 1058
PceI AGGCCT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PctI GAATGC 1 cut(s) 847
PfeI GAWTC 5 cut(s) 207, 657, 728, 761, 995
PfoI TCCNGGA 1 cut(s) 51
PkrI GCNGC 5 cut(s) 130, 189, 445, 864, 921
PleI GAGTC 2 cut(s) 755, 868
PpsI GAGTC 2 cut(s) 755, 868
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 2 cut(s) 550, 1112
PsiI TTATAA 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 324
Psp5II RGGWCCY 2 cut(s) 550, 1112
Psp6I CCWGG 1 cut(s) 531
PspFI CCCAGC 2 cut(s) 119, 376
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 4 cut(s) 438, 552, 1176, 1221
PspPI GGNCC 4 cut(s) 173, 445, 550, 1112
PspPPI RGGWCCY 2 cut(s) 550, 1112
PstI CTGCAG 1 cut(s) 545
PsuI RGATCY 3 cut(s) 246, 537, 1219
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 479, 767
SatI GCNGC 5 cut(s) 129, 188, 444, 863, 920
Sau3AI GATC 6 cut(s) 246, 517, 537, 591, 652, 1219
Sau96I GGNCC 4 cut(s) 173, 445, 550, 1112
SchI GAGTC 2 cut(s) 756, 868
ScrFI CCNGG 6 cut(s) 29, 53, 337, 338, 533, 554
SduI GDGCHC 3 cut(s) 313, 324, 960
SfaNI GCATC 3 cut(s) 199, 781, 849
SfcI CTRYAG 1 cut(s) 541
SinI GGWCC 2 cut(s) 550, 1112
SmaI CCCGGG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 317, 878, 953
SmoI CTYRAG 3 cut(s) 317, 878, 953
Sse9I AATT 1 cut(s) 46
SseBI AGGCCT 1 cut(s) 941
SsiI CCGC 6 cut(s) 129, 134, 187, 229, 291, 443
SstI GAGCTC 1 cut(s) 324
StuI AGGCCT 1 cut(s) 941
StyD4I CCNGG 6 cut(s) 27, 51, 335, 336, 531, 552
StyI CCWWGG 3 cut(s) 448, 732, 793
TaaI ACNGT 2 cut(s) 235, 1106
TaiI ACGT 1 cut(s) 697
TaqI TCGA 5 cut(s) 92, 520, 655, 839, 857
TasI AATT 1 cut(s) 46
TauI GCSGC 3 cut(s) 131, 190, 446
TfiI GAWTC 5 cut(s) 207, 657, 728, 761, 995
Tru1I TTAA 2 cut(s) 479, 767
Tru9I TTAA 2 cut(s) 479, 767
TscAI CASTG 2 cut(s) 1188, 1252
TseFI GTSAC 1 cut(s) 1058
TseI GCWGC 2 cut(s) 862, 919
Tsp45I GTSAC 1 cut(s) 1058
TspDTI ATGAA 5 cut(s) 77, 227, 481, 825, 1156
TspGWI ACGGA 3 cut(s) 486, 530, 1139
TspMI CCCGGG 1 cut(s) 336
TspRI CASTG 2 cut(s) 1188, 1252
VneI GTGCAC 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 550, 1112
XapI RAATTY 1 cut(s) 46
XmaI CCCGGG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.