Tether containing UBX domain for
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 632 |
| Acc36I | ACCTGC | 1 cut(s) | 1069 |
| Acc65I | GGTACC | 1 cut(s) | 1314 |
| AccB1I | GGYRCC | 1 cut(s) | 1314 |
| AccBSI | CCGCTC | 1 cut(s) | 1312 |
| AccI | GTMKAC | 1 cut(s) | 1057 |
| AciI | CCGC | 5 cut(s) | 281, 453, 620, 1035, 1312 |
| AclWI | GGATC | 4 cut(s) | 12, 25, 1563, 1576 |
| AcoI | YGGCCR | 2 cut(s) | 1032, 1204 |
| AcuI | CTGAAG | 3 cut(s) | 149, 1319, 1665 |
| AcyI | GRCGYC | 1 cut(s) | 943 |
| AfaI | GTAC | 6 cut(s) | 756, 923, 983, 1050, 1316, 1497 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1116, 1253, 1578, 1599 |
| AgsI | TTSAA | 3 cut(s) | 355, 662, 761 |
| AhlI | ACTAGT | 1 cut(s) | 1358 |
| AjnI | CCWGG | 3 cut(s) | 171, 292, 1571 |
| AluBI | AGCT | 6 cut(s) | 247, 379, 516, 1225, 1330, 1564 |
| AluI | AGCT | 6 cut(s) | 247, 379, 516, 1225, 1330, 1564 |
| Alw26I | GTCTC | 6 cut(s) | 73, 719, 805, 1078, 1139, 1185 |
| AlwI | GGATC | 4 cut(s) | 12, 25, 1563, 1576 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 172, 389 |
| Ama87I | CYCGRG | 1 cut(s) | 998 |
| AoxI | GGCC | 5 cut(s) | 30, 138, 781, 1032, 1204 |
| ApeKI | GCWGC | 4 cut(s) | 1222, 1229, 1232, 1294 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 771, 803, 1138, 1170 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 162 |
| Asp718I | GGTACC | 1 cut(s) | 1314 |
| AspLEI | GCGC | 1 cut(s) | 613 |
| AspS9I | GGNCC | 3 cut(s) | 323, 588, 1486 |
| AsuC2I | CCSGG | 1 cut(s) | 1078 |
| AsuHPI | GGTGA | 4 cut(s) | 308, 417, 534, 593 |
| AvaI | CYCGRG | 1 cut(s) | 998 |
| AvaII | GGWCC | 3 cut(s) | 323, 588, 1486 |
| AxyI | CCTNAGG | 1 cut(s) | 1546 |
| BaeGI | GKGCMC | 2 cut(s) | 906, 1638 |
| BalI | TGGCCA | 1 cut(s) | 1206 |
| BamHI | GGATCC | 2 cut(s) | 17, 1568 |
| BanI | GGYRCC | 1 cut(s) | 1314 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1362, 1394 |
| BauI | CACGAG | 1 cut(s) | 1516 |
| BbsI | GAAGAC | 4 cut(s) | 630, 1273, 1366, 1413 |
| BbvI | GCAGC | 4 cut(s) | 1216, 1234, 1244, 1281 |
| BccI | CCATC | 7 cut(s) | 156, 160, 701, 922, 1109, 1438, 1552 |
| BceAI | ACGGC | 2 cut(s) | 1047, 1454 |
| BciT130I | CCWGG | 3 cut(s) | 173, 294, 1573 |
| BclI | TGATCA | 1 cut(s) | 102 |
| BcnI | CCSGG | 1 cut(s) | 1078 |
| BcoDI | GTCTC | 6 cut(s) | 73, 719, 805, 1078, 1139, 1185 |
| BcuI | ACTAGT | 1 cut(s) | 1358 |
| BfaI | CTAG | 3 cut(s) | 575, 785, 1359 |
| BfmI | CTRYAG | 3 cut(s) | 306, 561, 1230 |
| BfuAI | ACCTGC | 1 cut(s) | 1069 |
| BglII | AGATCT | 1 cut(s) | 1019 |
| BisI | GCNGC | 5 cut(s) | 1035, 1223, 1230, 1233, 1295 |
| BlsI | GCNGC | 5 cut(s) | 1036, 1224, 1231, 1234, 1296 |
| Bme1390I | CCNGG | 4 cut(s) | 173, 294, 1078, 1573 |
| Bme18I | GGWCC | 3 cut(s) | 323, 588, 1486 |
| BmeT110I | CYCGRG | 1 cut(s) | 998 |
| BmgT120I | GGNCC | 3 cut(s) | 323, 588, 1486 |
| BmiI | GGNNCC | 5 cut(s) | 19, 681, 1316, 1570, 1583 |
| BmrFI | CCNGG | 4 cut(s) | 173, 294, 1078, 1573 |
| BmrI | ACTGGG | 1 cut(s) | 921 |
| BmsI | GCATC | 4 cut(s) | 431, 755, 1134, 1489 |
| BmuI | ACTGGG | 1 cut(s) | 921 |
| BpiI | GAAGAC | 4 cut(s) | 630, 1273, 1366, 1413 |
| BpmI | CTGGAG | 3 cut(s) | 600, 1330, 1594 |
| BpuEI | CTTGAG | 4 cut(s) | 743, 829, 940, 1328 |
| BpuMI | CCSGG | 1 cut(s) | 1078 |
| BsaAI | YACGTR | 1 cut(s) | 754 |
| BsaHI | GRCGYC | 1 cut(s) | 943 |
| BsaJI | CCNNGG | 7 cut(s) | 10, 93, 584, 684, 1207, 1383, 1489 |
| BsaWI | WCCGGW | 1 cut(s) | 403 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 220, 250, 703, 721, 733, 751 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1116, 1253, 1578, 1599 |
| Bse1I | ACTGG | 4 cut(s) | 916, 1258, 1347, 1439 |
| Bse21I | CCTNAGG | 1 cut(s) | 1546 |
| Bse3DI | GCAATG | 1 cut(s) | 1128 |
| BseBI | CCWGG | 3 cut(s) | 173, 294, 1573 |
| BseDI | CCNNGG | 7 cut(s) | 10, 93, 584, 684, 1207, 1383, 1489 |
| BseGI | GGATG | 2 cut(s) | 148, 1165 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1116, 1253, 1578, 1599 |
| BseMI | GCAATG | 1 cut(s) | 1128 |
| BseMII | CTCAG | 3 cut(s) | 1014, 1140, 1560 |
| BseNI | ACTGG | 4 cut(s) | 916, 1258, 1347, 1439 |
| BseRI | GAGGAG | 6 cut(s) | 27, 173, 225, 1030, 1591, 1598 |
| BseSI | GKGCMC | 2 cut(s) | 906, 1638 |
| BseX3I | CGGCCG | 1 cut(s) | 1032 |
| BseXI | GCAGC | 4 cut(s) | 1216, 1234, 1244, 1281 |
| BsgI | GTGCAG | 2 cut(s) | 384, 1383 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1035 |
| BshFI | GGCC | 5 cut(s) | 32, 140, 783, 1034, 1206 |
| BshNI | GGYRCC | 1 cut(s) | 1314 |
| BsiEI | CGRYCG | 1 cut(s) | 1035 |
| BsiHKCI | CYCGRG | 1 cut(s) | 998 |
| BsiSI | CCGG | 2 cut(s) | 404, 1078 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1116, 1253, 1578, 1599 |
| BsmAI | GTCTC | 6 cut(s) | 73, 719, 805, 1078, 1139, 1185 |
| BsmBI | CGTCTC | 1 cut(s) | 73 |
| BsmI | GAATGC | 1 cut(s) | 1557 |
| BsnI | GGCC | 5 cut(s) | 32, 140, 783, 1034, 1206 |
| BsoBI | CYCGRG | 1 cut(s) | 998 |
| Bsp1286I | GDGCHC | 2 cut(s) | 906, 1638 |
| Bsp1407I | TGTACA | 2 cut(s) | 921, 1048 |
| Bsp143I | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| Bsp19I | CCATGG | 2 cut(s) | 1207, 1489 |
| BspACI | CCGC | 5 cut(s) | 281, 453, 620, 1035, 1312 |
| BspANI | GGCC | 5 cut(s) | 32, 140, 783, 1034, 1206 |
| BspCNI | CTCAG | 3 cut(s) | 1015, 1141, 1559 |
| BspHI | TCATGA | 1 cut(s) | 186 |
| BspLI | GGNNCC | 5 cut(s) | 19, 681, 1316, 1570, 1583 |
| BspMAI | CTGCAG | 2 cut(s) | 310, 1234 |
| BspMI | ACCTGC | 1 cut(s) | 1069 |
| BspPI | GGATC | 4 cut(s) | 12, 25, 1563, 1576 |
| BspQI | GCTCTTC | 1 cut(s) | 831 |
| BspT107I | GGYRCC | 1 cut(s) | 1314 |
| BsrBI | CCGCTC | 1 cut(s) | 1312 |
| BsrDI | GCAATG | 1 cut(s) | 1128 |
| BsrGI | TGTACA | 2 cut(s) | 921, 1048 |
| BsrI | ACTGG | 4 cut(s) | 916, 1258, 1347, 1439 |
| BssECI | CCNNGG | 7 cut(s) | 10, 93, 584, 684, 1207, 1383, 1489 |
| BssMI | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| BssNI | GRCGYC | 1 cut(s) | 943 |
| BssSI | CACGAG | 1 cut(s) | 1516 |
| BssT1I | CCWWGG | 5 cut(s) | 93, 684, 1207, 1383, 1489 |
| Bst2BI | CACGAG | 1 cut(s) | 1516 |
| Bst2UI | CCWGG | 3 cut(s) | 173, 294, 1573 |
| Bst4CI | ACNGT | 5 cut(s) | 629, 719, 847, 986, 1319 |
| Bst6I | CTCTTC | 6 cut(s) | 28, 168, 333, 511, 831, 1641 |
| BstACI | GRCGYC | 1 cut(s) | 943 |
| BstAUI | TGTACA | 2 cut(s) | 921, 1048 |
| BstBAI | YACGTR | 1 cut(s) | 754 |
| BstC8I | GCNNGC | 5 cut(s) | 283, 381, 420, 453, 1227 |
| BstDEI | CTNAG | 5 cut(s) | 240, 1023, 1141, 1149, 1546 |
| BstDSI | CCRYGG | 3 cut(s) | 584, 1207, 1489 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1576 |
| BstF5I | GGATG | 2 cut(s) | 148, 1165 |
| BstHHI | GCGC | 1 cut(s) | 613 |
| BstKTI | GATC | 7 cut(s) | 20, 105, 181, 345, 1022, 1419, 1571 |
| BstMAI | GTCTC | 6 cut(s) | 73, 719, 805, 1078, 1139, 1185 |
| BstMBI | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| BstMCI | CGRYCG | 1 cut(s) | 1035 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 287, 385, 407, 617, 1561, 1633 |
| BstNI | CCWGG | 3 cut(s) | 173, 294, 1573 |
| BstNSI | RCATGY | 2 cut(s) | 698, 1041 |
| BstSCI | CCNGG | 4 cut(s) | 171, 292, 1076, 1571 |
| BstSFI | CTRYAG | 3 cut(s) | 306, 561, 1230 |
| BstSLI | GKGCMC | 2 cut(s) | 906, 1638 |
| BstSNI | TACGTA | 1 cut(s) | 754 |
| BstV1I | GCAGC | 4 cut(s) | 1216, 1234, 1244, 1281 |
| BstV2I | GAAGAC | 4 cut(s) | 630, 1273, 1366, 1413 |
| BstX2I | RGATCY | 3 cut(s) | 17, 1019, 1568 |
| BstYI | RGATCY | 3 cut(s) | 17, 1019, 1568 |
| BstZI | CGGCCG | 1 cut(s) | 1032 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1546 |
| BsuRI | GGCC | 5 cut(s) | 32, 140, 783, 1034, 1206 |
| BtgI | CCRYGG | 3 cut(s) | 584, 1207, 1489 |
| BtsCI | GGATG | 2 cut(s) | 148, 1165 |
| BtsIMutI | CAGTG | 2 cut(s) | 1354, 1432 |
| BveI | ACCTGC | 1 cut(s) | 1069 |
| Cac8I | GCNNGC | 5 cut(s) | 283, 381, 420, 453, 1227 |
| CaiI | CAGNNNCTG | 2 cut(s) | 172, 389 |
| CciI | TCATGA | 1 cut(s) | 186 |
| CfoI | GCGC | 1 cut(s) | 613 |
| Cfr13I | GGNCC | 3 cut(s) | 323, 588, 1486 |
| CseI | GACGC | 1 cut(s) | 951 |
| Csp6I | GTAC | 6 cut(s) | 755, 922, 982, 1049, 1315, 1496 |
| CviQI | GTAC | 6 cut(s) | 755, 922, 982, 1049, 1315, 1496 |
| DdeI | CTNAG | 5 cut(s) | 240, 1023, 1141, 1149, 1546 |
| DpnI | GATC | 7 cut(s) | 19, 104, 180, 344, 1021, 1418, 1570 |
| DpnII | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 632 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 632 |
| EaeI | YGGCCR | 2 cut(s) | 1032, 1204 |
| EagI | CGGCCG | 1 cut(s) | 1032 |
| Eam1104I | CTCTTC | 6 cut(s) | 28, 168, 333, 511, 831, 1641 |
| EarI | CTCTTC | 6 cut(s) | 28, 168, 333, 511, 831, 1641 |
| EclXI | CGGCCG | 1 cut(s) | 1032 |
| Eco105I | TACGTA | 1 cut(s) | 754 |
| Eco130I | CCWWGG | 5 cut(s) | 93, 684, 1207, 1383, 1489 |
| Eco147I | AGGCCT | 1 cut(s) | 783 |
| Eco32I | GATATC | 2 cut(s) | 668, 1528 |
| Eco47I | GGWCC | 3 cut(s) | 323, 588, 1486 |
| Eco52I | CGGCCG | 1 cut(s) | 1032 |
| Eco57I | CTGAAG | 3 cut(s) | 149, 1319, 1665 |
| Eco81I | CCTNAGG | 1 cut(s) | 1546 |
| Eco88I | CYCGRG | 1 cut(s) | 998 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1576 |
| EcoO109I | RGGNCCY | 1 cut(s) | 323 |
| EcoRII | CCWGG | 3 cut(s) | 171, 292, 1571 |
| EcoRV | GATATC | 2 cut(s) | 668, 1528 |
| EcoT14I | CCWWGG | 5 cut(s) | 93, 684, 1207, 1383, 1489 |
| EcoT22I | ATGCAT | 1 cut(s) | 824 |
| ErhI | CCWWGG | 5 cut(s) | 93, 684, 1207, 1383, 1489 |
| Esp3I | CGTCTC | 1 cut(s) | 73 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 639, 671, 1258, 1290 |
| FauI | CCCGC | 2 cut(s) | 274, 460 |
| FauNDI | CATATG | 1 cut(s) | 1069 |
| FbaI | TGATCA | 1 cut(s) | 102 |
| FblI | GTMKAC | 1 cut(s) | 1057 |
| Fnu4HI | GCNGC | 5 cut(s) | 1035, 1223, 1230, 1233, 1295 |
| FokI | GGATG | 2 cut(s) | 135, 1172 |
| Fsp4HI | GCNGC | 5 cut(s) | 1035, 1223, 1230, 1233, 1295 |
| FspBI | CTAG | 3 cut(s) | 575, 785, 1359 |
| GlaI | GCGC | 1 cut(s) | 612 |
| GluI | GCNGC | 5 cut(s) | 1035, 1223, 1230, 1233, 1295 |
| GsuI | CTGGAG | 3 cut(s) | 600, 1330, 1594 |
| HaeIII | GGCC | 5 cut(s) | 32, 140, 783, 1034, 1206 |
| HapII | CCGG | 2 cut(s) | 404, 1078 |
| HgaI | GACGC | 1 cut(s) | 951 |
| HhaI | GCGC | 1 cut(s) | 613 |
| Hin1I | GRCGYC | 1 cut(s) | 943 |
| Hin6I | GCGC | 1 cut(s) | 611 |
| HinP1I | GCGC | 1 cut(s) | 611 |
| HinfI | GANTC | 9 cut(s) | 52, 500, 535, 964, 1081, 1269, 1603, 1640, 1674 |
| HpaII | CCGG | 2 cut(s) | 404, 1078 |
| HphI | GGTGA | 4 cut(s) | 308, 417, 534, 593 |
| Hpy166II | GTNNAC | 3 cut(s) | 1058, 1354, 1496 |
| Hpy188I | TCNGA | 8 cut(s) | 37, 157, 168, 396, 706, 797, 1024, 1645 |
| Hpy8I | GTNNAC | 3 cut(s) | 1058, 1354, 1496 |
| HpyAV | CCTTC | 4 cut(s) | 341, 614, 872, 1294 |
| HpyCH4III | ACNGT | 5 cut(s) | 629, 719, 847, 986, 1319 |
| HpyCH4IV | ACGT | 2 cut(s) | 753, 1009 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 287, 385, 407, 617, 1561, 1633 |
| HpyF3I | CTNAG | 5 cut(s) | 240, 1023, 1141, 1149, 1546 |
| HpySE526I | ACGT | 2 cut(s) | 753, 1009 |
| Hsp92I | GRCGYC | 1 cut(s) | 943 |
| HspAI | GCGC | 1 cut(s) | 611 |
| KpnI | GGTACC | 1 cut(s) | 1318 |
| Ksp22I | TGATCA | 1 cut(s) | 102 |
| Kzo9I | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| LguI | GCTCTTC | 1 cut(s) | 831 |
| LmnI | GCTCC | 4 cut(s) | 295, 521, 1412, 1561 |
| Lsp1109I | GCAGC | 4 cut(s) | 1216, 1234, 1244, 1281 |
| LweI | GCATC | 4 cut(s) | 431, 755, 1134, 1489 |
| MaeI | CTAG | 3 cut(s) | 575, 785, 1359 |
| MaeII | ACGT | 2 cut(s) | 753, 1009 |
| MaeIII | GTNAC | 3 cut(s) | 713, 1027, 1333 |
| MalI | GATC | 7 cut(s) | 19, 104, 180, 344, 1021, 1418, 1570 |
| MbiI | CCGCTC | 1 cut(s) | 1312 |
| MboI | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| MflI | RGATCY | 3 cut(s) | 17, 1019, 1568 |
| MhlI | GDGCHC | 2 cut(s) | 906, 1638 |
| MlsI | TGGCCA | 1 cut(s) | 1206 |
| MluCI | AATT | 2 cut(s) | 209, 1163 |
| MluNI | TGGCCA | 1 cut(s) | 1206 |
| MlyI | GAGTC | 1 cut(s) | 1263 |
| MmeI | TCCRAC | 1 cut(s) | 992 |
| Mox20I | TGGCCA | 1 cut(s) | 1206 |
| Mph1103I | ATGCAT | 1 cut(s) | 824 |
| MroXI | GAANNNNTTC | 1 cut(s) | 162 |
| MscI | TGGCCA | 1 cut(s) | 1206 |
| MseI | TTAA | 3 cut(s) | 1179, 1215, 1502 |
| MslI | CAYNNNNRTG | 2 cut(s) | 191, 1158 |
| Msp20I | TGGCCA | 1 cut(s) | 1206 |
| MspA1I | CMGCKG | 1 cut(s) | 620 |
| MspI | CCGG | 2 cut(s) | 404, 1078 |
| MspR9I | CCNGG | 4 cut(s) | 173, 294, 1078, 1573 |
| Mva1269I | GAATGC | 1 cut(s) | 1557 |
| MvaI | CCWGG | 3 cut(s) | 173, 294, 1573 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 287, 385, 407, 617, 1561, 1633 |
| NciI | CCSGG | 1 cut(s) | 1078 |
| NcoI | CCATGG | 2 cut(s) | 1207, 1489 |
| NdeI | CATATG | 1 cut(s) | 1069 |
| NdeII | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| NlaIV | GGNNCC | 5 cut(s) | 19, 681, 1316, 1570, 1583 |
| NmeAIII | GCCGAG | 1 cut(s) | 35 |
| NmuCI | GTSAC | 1 cut(s) | 1333 |
| NsiI | ATGCAT | 1 cut(s) | 824 |
| NspI | RCATGY | 2 cut(s) | 698, 1041 |
| PaeR7I | CTCGAG | 1 cut(s) | 998 |
| PagI | TCATGA | 1 cut(s) | 186 |
| PceI | AGGCCT | 1 cut(s) | 783 |
| PciSI | GCTCTTC | 1 cut(s) | 831 |
| PctI | GAATGC | 1 cut(s) | 1557 |
| PdmI | GAANNNNTTC | 1 cut(s) | 162 |
| PfeI | GAWTC | 8 cut(s) | 52, 500, 535, 964, 1081, 1603, 1640, 1674 |
| PfoI | TCCNGGA | 2 cut(s) | 1076, 1571 |
| PkrI | GCNGC | 5 cut(s) | 1036, 1224, 1231, 1234, 1296 |
| PleI | GAGTC | 1 cut(s) | 1263 |
| PpsI | GAGTC | 1 cut(s) | 1263 |
| Ppu21I | YACGTR | 1 cut(s) | 754 |
| PpuMI | RGGWCCY | 1 cut(s) | 323 |
| Psp5II | RGGWCCY | 1 cut(s) | 323 |
| Psp6I | CCWGG | 3 cut(s) | 171, 292, 1571 |
| PspGI | CCWGG | 3 cut(s) | 171, 292, 1571 |
| PspN4I | GGNNCC | 5 cut(s) | 19, 681, 1316, 1570, 1583 |
| PspPI | GGNCC | 3 cut(s) | 323, 588, 1486 |
| PspPPI | RGGWCCY | 1 cut(s) | 323 |
| PstI | CTGCAG | 2 cut(s) | 310, 1234 |
| PstNI | CAGNNNCTG | 2 cut(s) | 172, 389 |
| PsuI | RGATCY | 3 cut(s) | 17, 1019, 1568 |
| RsaI | GTAC | 6 cut(s) | 756, 923, 983, 1050, 1316, 1497 |
| RsaNI | GTAC | 6 cut(s) | 755, 922, 982, 1049, 1315, 1496 |
| RseI | CAYNNNNRTG | 2 cut(s) | 191, 1158 |
| SapI | GCTCTTC | 1 cut(s) | 831 |
| SaqAI | TTAA | 3 cut(s) | 1179, 1215, 1502 |
| SatI | GCNGC | 5 cut(s) | 1035, 1223, 1230, 1233, 1295 |
| Sau3AI | GATC | 7 cut(s) | 17, 102, 178, 342, 1019, 1416, 1568 |
| Sau96I | GGNCC | 3 cut(s) | 323, 588, 1486 |
| SchI | GAGTC | 1 cut(s) | 1263 |
| ScrFI | CCNGG | 4 cut(s) | 173, 294, 1078, 1573 |
| SduI | GDGCHC | 2 cut(s) | 906, 1638 |
| SfaNI | GCATC | 4 cut(s) | 431, 755, 1134, 1489 |
| SfcI | CTRYAG | 3 cut(s) | 306, 561, 1230 |
| Sfr274I | CTCGAG | 1 cut(s) | 998 |
| SinI | GGWCC | 3 cut(s) | 323, 588, 1486 |
| SlaI | CTCGAG | 1 cut(s) | 998 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 191, 1158 |
| SmlI | CTYRAG | 5 cut(s) | 722, 808, 955, 998, 1307 |
| SmoI | CTYRAG | 5 cut(s) | 722, 808, 955, 998, 1307 |
| SnaBI | TACGTA | 1 cut(s) | 754 |
| SpeI | ACTAGT | 1 cut(s) | 1358 |
| Sse9I | AATT | 2 cut(s) | 209, 1163 |
| SseBI | AGGCCT | 1 cut(s) | 783 |
| SsiI | CCGC | 5 cut(s) | 281, 453, 620, 1035, 1312 |
| SspMI | CTAG | 3 cut(s) | 575, 785, 1359 |
| StuI | AGGCCT | 1 cut(s) | 783 |
| StyD4I | CCNGG | 4 cut(s) | 171, 292, 1076, 1571 |
| StyI | CCWWGG | 5 cut(s) | 93, 684, 1207, 1383, 1489 |
| TaaI | ACNGT | 5 cut(s) | 629, 719, 847, 986, 1319 |
| TaiI | ACGT | 2 cut(s) | 756, 1012 |
| TaqI | TCGA | 3 cut(s) | 202, 999, 1619 |
| TasI | AATT | 2 cut(s) | 209, 1163 |
| TatI | WGTACW | 2 cut(s) | 921, 1048 |
| TauI | GCSGC | 1 cut(s) | 1037 |
| TfiI | GAWTC | 8 cut(s) | 52, 500, 535, 964, 1081, 1603, 1640, 1674 |
| Tru1I | TTAA | 3 cut(s) | 1179, 1215, 1502 |
| Tru9I | TTAA | 3 cut(s) | 1179, 1215, 1502 |
| TscAI | CASTG | 2 cut(s) | 1354, 1439 |
| TseFI | GTSAC | 1 cut(s) | 1333 |
| TseI | GCWGC | 4 cut(s) | 1222, 1229, 1232, 1294 |
| Tsp45I | GTSAC | 1 cut(s) | 1333 |
| TspDTI | ATGAA | 7 cut(s) | 15, 17, 203, 888, 1176, 1366, 1383 |
| TspGWI | ACGGA | 2 cut(s) | 601, 1425 |
| TspRI | CASTG | 2 cut(s) | 1354, 1439 |
| VpaK11BI | GGWCC | 3 cut(s) | 323, 588, 1486 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1576 |
| XbaI | TCTAGA | 1 cut(s) | 574 |
| XceI | RCATGY | 2 cut(s) | 698, 1041 |
| XhoI | CTCGAG | 1 cut(s) | 998 |
| XmiI | GTMKAC | 1 cut(s) | 1057 |
| XmnI | GAANNNNTTC | 1 cut(s) | 162 |
| XspI | CTAG | 3 cut(s) | 575, 785, 1359 |
| Zsp2I | ATGCAT | 1 cut(s) | 824 |