4-coumarate--CoA ligase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 562 |
| AciI | CCGC | 2 cut(s) | 626, 829 |
| AcsI | RAATTY | 1 cut(s) | 197 |
| AcuI | CTGAAG | 1 cut(s) | 660 |
| AfaI | GTAC | 1 cut(s) | 672 |
| AgsI | TTSAA | 6 cut(s) | 136, 297, 323, 421, 578, 742 |
| AjnI | CCWGG | 2 cut(s) | 90, 555 |
| AleI | CACNNNNGTG | 1 cut(s) | 61 |
| AluBI | AGCT | 8 cut(s) | 158, 218, 243, 535, 569, 644, 714, 814 |
| AluI | AGCT | 8 cut(s) | 158, 218, 243, 535, 569, 644, 714, 814 |
| Alw21I | GWGCWC | 1 cut(s) | 816 |
| Alw26I | GTCTC | 1 cut(s) | 437 |
| Ama87I | CYCGRG | 1 cut(s) | 17 |
| AoxI | GGCC | 1 cut(s) | 394 |
| ApaI | GGGCCC | 1 cut(s) | 398 |
| ApeKI | GCWGC | 2 cut(s) | 155, 583 |
| ApoI | RAATTY | 1 cut(s) | 197 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 253 |
| AspS9I | GGNCC | 3 cut(s) | 394, 395, 652 |
| AsuC2I | CCSGG | 1 cut(s) | 428 |
| AsuHPI | GGTGA | 2 cut(s) | 587, 659 |
| AvaI | CYCGRG | 1 cut(s) | 17 |
| AvaII | GGWCC | 1 cut(s) | 652 |
| BaeGI | GKGCMC | 1 cut(s) | 398 |
| BanI | GGYRCC | 1 cut(s) | 562 |
| BanII | GRGCYC | 2 cut(s) | 398, 816 |
| BbsI | GAAGAC | 1 cut(s) | 29 |
| Bbv12I | GWGCWC | 1 cut(s) | 816 |
| BbvI | GCAGC | 2 cut(s) | 167, 570 |
| BccI | CCATC | 3 cut(s) | 25, 172, 400 |
| BciT130I | CCWGG | 2 cut(s) | 92, 557 |
| BciVI | GTATCC | 1 cut(s) | 479 |
| BcnI | CCSGG | 1 cut(s) | 428 |
| BcoDI | GTCTC | 1 cut(s) | 437 |
| BfaI | CTAG | 2 cut(s) | 399, 587 |
| BfmI | CTRYAG | 2 cut(s) | 78, 447 |
| BfuI | GTATCC | 1 cut(s) | 479 |
| BglII | AGATCT | 1 cut(s) | 478 |
| BisI | GCNGC | 2 cut(s) | 156, 584 |
| BlsI | GCNGC | 2 cut(s) | 157, 585 |
| Bme1390I | CCNGG | 3 cut(s) | 92, 428, 557 |
| Bme18I | GGWCC | 1 cut(s) | 652 |
| BmeT110I | CYCGRG | 1 cut(s) | 17 |
| BmgT120I | GGNCC | 3 cut(s) | 394, 395, 652 |
| BmiI | GGNNCC | 4 cut(s) | 395, 396, 564, 654 |
| BmrFI | CCNGG | 3 cut(s) | 92, 428, 557 |
| BmsI | GCATC | 3 cut(s) | 625, 743, 797 |
| BpiI | GAAGAC | 1 cut(s) | 29 |
| Bpu10I | CCTNAGC | 1 cut(s) | 219 |
| BpuEI | CTTGAG | 1 cut(s) | 189 |
| BpuMI | CCSGG | 1 cut(s) | 428 |
| BsaJI | CCNNGG | 1 cut(s) | 90 |
| Bse1I | ACTGG | 2 cut(s) | 295, 304 |
| BseBI | CCWGG | 2 cut(s) | 92, 557 |
| BseDI | CCNNGG | 1 cut(s) | 90 |
| BseGI | GGATG | 1 cut(s) | 621 |
| BseMII | CTCAG | 2 cut(s) | 233, 817 |
| BseNI | ACTGG | 2 cut(s) | 295, 304 |
| BseRI | GAGGAG | 1 cut(s) | 197 |
| BseSI | GKGCMC | 1 cut(s) | 398 |
| BseXI | GCAGC | 2 cut(s) | 167, 570 |
| BseYI | CCCAGC | 1 cut(s) | 410 |
| Bsh1285I | CGRYCG | 1 cut(s) | 833 |
| BshFI | GGCC | 1 cut(s) | 396 |
| BshNI | GGYRCC | 1 cut(s) | 562 |
| BsiEI | CGRYCG | 1 cut(s) | 833 |
| BsiHKAI | GWGCWC | 1 cut(s) | 816 |
| BsiHKCI | CYCGRG | 1 cut(s) | 17 |
| BsiSI | CCGG | 1 cut(s) | 428 |
| BsiWI | CGTACG | 1 cut(s) | 670 |
| BslFI | GGGAC | 4 cut(s) | 292, 516, 638, 761 |
| BsmAI | GTCTC | 1 cut(s) | 437 |
| BsmFI | GGGAC | 4 cut(s) | 292, 516, 638, 761 |
| BsnI | GGCC | 1 cut(s) | 396 |
| BsoBI | CYCGRG | 1 cut(s) | 17 |
| Bsp120I | GGGCCC | 1 cut(s) | 394 |
| Bsp1286I | GDGCHC | 2 cut(s) | 398, 816 |
| Bsp143I | GATC | 3 cut(s) | 13, 478, 520 |
| BspACI | CCGC | 2 cut(s) | 626, 829 |
| BspANI | GGCC | 1 cut(s) | 396 |
| BspCNI | CTCAG | 2 cut(s) | 232, 816 |
| BspLI | GGNNCC | 4 cut(s) | 395, 396, 564, 654 |
| BspT107I | GGYRCC | 1 cut(s) | 562 |
| BsrI | ACTGG | 2 cut(s) | 295, 304 |
| BssECI | CCNNGG | 1 cut(s) | 90 |
| BssMI | GATC | 3 cut(s) | 13, 478, 520 |
| Bst2UI | CCWGG | 2 cut(s) | 92, 557 |
| Bst4CI | ACNGT | 2 cut(s) | 104, 372 |
| Bst6I | CTCTTC | 2 cut(s) | 748, 802 |
| BstC8I | GCNNGC | 3 cut(s) | 87, 273, 677 |
| BstDEI | CTNAG | 2 cut(s) | 219, 803 |
| BstF5I | GGATG | 1 cut(s) | 621 |
| BstKTI | GATC | 3 cut(s) | 16, 481, 523 |
| BstMAI | GTCTC | 1 cut(s) | 437 |
| BstMBI | GATC | 3 cut(s) | 13, 478, 520 |
| BstMCI | CGRYCG | 1 cut(s) | 833 |
| BstNI | CCWGG | 2 cut(s) | 92, 557 |
| BstSCI | CCNGG | 3 cut(s) | 90, 426, 555 |
| BstSFI | CTRYAG | 2 cut(s) | 78, 447 |
| BstSLI | GKGCMC | 1 cut(s) | 398 |
| BstV1I | GCAGC | 2 cut(s) | 167, 570 |
| BstV2I | GAAGAC | 1 cut(s) | 29 |
| BstX2I | RGATCY | 1 cut(s) | 478 |
| BstYI | RGATCY | 1 cut(s) | 478 |
| BsuI | GTATCC | 1 cut(s) | 479 |
| BsuRI | GGCC | 1 cut(s) | 396 |
| BtsCI | GGATG | 1 cut(s) | 621 |
| Cac8I | GCNNGC | 3 cut(s) | 87, 273, 677 |
| Cfr13I | GGNCC | 3 cut(s) | 394, 395, 652 |
| Csp6I | GTAC | 1 cut(s) | 671 |
| CviQI | GTAC | 1 cut(s) | 671 |
| DdeI | CTNAG | 2 cut(s) | 219, 803 |
| DpnI | GATC | 3 cut(s) | 15, 480, 522 |
| DpnII | GATC | 3 cut(s) | 13, 478, 520 |
| Eam1104I | CTCTTC | 2 cut(s) | 748, 802 |
| EarI | CTCTTC | 2 cut(s) | 748, 802 |
| Ecl136II | GAGCTC | 1 cut(s) | 814 |
| Eco24I | GRGCYC | 2 cut(s) | 398, 816 |
| Eco47I | GGWCC | 1 cut(s) | 652 |
| Eco53kI | GAGCTC | 1 cut(s) | 814 |
| Eco57I | CTGAAG | 1 cut(s) | 660 |
| Eco88I | CYCGRG | 1 cut(s) | 17 |
| EcoICRI | GAGCTC | 1 cut(s) | 814 |
| EcoO109I | RGGNCCY | 3 cut(s) | 394, 395, 652 |
| EcoRII | CCWGG | 2 cut(s) | 90, 555 |
| EcoT38I | GRGCYC | 2 cut(s) | 398, 816 |
| FaqI | GGGAC | 4 cut(s) | 292, 516, 638, 761 |
| FauI | CCCGC | 1 cut(s) | 633 |
| Fnu4HI | GCNGC | 2 cut(s) | 156, 584 |
| FokI | GGATG | 1 cut(s) | 608 |
| FriOI | GRGCYC | 2 cut(s) | 398, 816 |
| Fsp4HI | GCNGC | 2 cut(s) | 156, 584 |
| FspBI | CTAG | 2 cut(s) | 399, 587 |
| GluI | GCNGC | 2 cut(s) | 156, 584 |
| GsaI | CCCAGC | 1 cut(s) | 414 |
| HaeIII | GGCC | 1 cut(s) | 396 |
| HapII | CCGG | 1 cut(s) | 428 |
| HinfI | GANTC | 3 cut(s) | 280, 525, 611 |
| HpaII | CCGG | 1 cut(s) | 428 |
| HphI | GGTGA | 2 cut(s) | 587, 659 |
| Hpy166II | GTNNAC | 1 cut(s) | 262 |
| Hpy188I | TCNGA | 3 cut(s) | 330, 693, 806 |
| Hpy188III | TCNNGA | 6 cut(s) | 17, 19, 315, 632, 709, 820 |
| Hpy8I | GTNNAC | 1 cut(s) | 262 |
| Hpy99I | CGWCG | 1 cut(s) | 133 |
| HpyAV | CCTTC | 1 cut(s) | 572 |
| HpyCH4III | ACNGT | 2 cut(s) | 104, 372 |
| HpyCH4V | TGCA | 3 cut(s) | 191, 662, 734 |
| HpyF3I | CTNAG | 2 cut(s) | 219, 803 |
| Kzo9I | GATC | 3 cut(s) | 13, 478, 520 |
| LmnI | GCTCC | 1 cut(s) | 163 |
| Lsp1109I | GCAGC | 2 cut(s) | 167, 570 |
| LweI | GCATC | 3 cut(s) | 625, 743, 797 |
| MaeI | CTAG | 2 cut(s) | 399, 587 |
| MaeIII | GTNAC | 3 cut(s) | 52, 465, 757 |
| MalI | GATC | 3 cut(s) | 15, 480, 522 |
| MboI | GATC | 3 cut(s) | 13, 478, 520 |
| MboII | GAAGA | 5 cut(s) | 34, 710, 713, 765, 819 |
| MflI | RGATCY | 1 cut(s) | 478 |
| MhlI | GDGCHC | 2 cut(s) | 398, 816 |
| MluCI | AATT | 3 cut(s) | 197, 249, 318 |
| MlyI | GAGTC | 1 cut(s) | 274 |
| MnlI | CCTC | 8 cut(s) | 84, 175, 213, 366, 372, 424, 643, 812 |
| MroXI | GAANNNNTTC | 1 cut(s) | 253 |
| MseI | TTAA | 3 cut(s) | 111, 120, 540 |
| MslI | CAYNNNNRTG | 1 cut(s) | 61 |
| MspA1I | CMGCKG | 1 cut(s) | 569 |
| MspI | CCGG | 1 cut(s) | 428 |
| MspR9I | CCNGG | 3 cut(s) | 92, 428, 557 |
| MvaI | CCWGG | 2 cut(s) | 92, 557 |
| NciI | CCSGG | 1 cut(s) | 428 |
| NdeII | GATC | 3 cut(s) | 13, 478, 520 |
| NlaIV | GGNNCC | 4 cut(s) | 395, 396, 564, 654 |
| OliI | CACNNNNGTG | 1 cut(s) | 61 |
| PaeR7I | CTCGAG | 1 cut(s) | 17 |
| PdmI | GAANNNNTTC | 1 cut(s) | 253 |
| PfeI | GAWTC | 2 cut(s) | 525, 611 |
| Pfl23II | CGTACG | 1 cut(s) | 670 |
| PkrI | GCNGC | 2 cut(s) | 157, 585 |
| PleI | GAGTC | 1 cut(s) | 274 |
| PpsI | GAGTC | 1 cut(s) | 274 |
| PpuMI | RGGWCCY | 1 cut(s) | 652 |
| Psp124BI | GAGCTC | 1 cut(s) | 816 |
| Psp5II | RGGWCCY | 1 cut(s) | 652 |
| Psp6I | CCWGG | 2 cut(s) | 90, 555 |
| PspFI | CCCAGC | 1 cut(s) | 410 |
| PspGI | CCWGG | 2 cut(s) | 90, 555 |
| PspLI | CGTACG | 1 cut(s) | 670 |
| PspN4I | GGNNCC | 4 cut(s) | 395, 396, 564, 654 |
| PspOMI | GGGCCC | 1 cut(s) | 394 |
| PspPI | GGNCC | 3 cut(s) | 394, 395, 652 |
| PspPPI | RGGWCCY | 1 cut(s) | 652 |
| PsuI | RGATCY | 1 cut(s) | 478 |
| PvuII | CAGCTG | 1 cut(s) | 569 |
| RsaI | GTAC | 1 cut(s) | 672 |
| RsaNI | GTAC | 1 cut(s) | 671 |
| RseI | CAYNNNNRTG | 1 cut(s) | 61 |
| SacI | GAGCTC | 1 cut(s) | 816 |
| SaqAI | TTAA | 3 cut(s) | 111, 120, 540 |
| SatI | GCNGC | 2 cut(s) | 156, 584 |
| Sau3AI | GATC | 3 cut(s) | 13, 478, 520 |
| Sau96I | GGNCC | 3 cut(s) | 394, 395, 652 |
| SchI | GAGTC | 1 cut(s) | 274 |
| ScrFI | CCNGG | 3 cut(s) | 92, 428, 557 |
| SduI | GDGCHC | 2 cut(s) | 398, 816 |
| SfaNI | GCATC | 3 cut(s) | 625, 743, 797 |
| SfcI | CTRYAG | 2 cut(s) | 78, 447 |
| Sfr274I | CTCGAG | 1 cut(s) | 17 |
| SinI | GGWCC | 1 cut(s) | 652 |
| SlaI | CTCGAG | 1 cut(s) | 17 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 61 |
| SmlI | CTYRAG | 2 cut(s) | 17, 204 |
| SmoI | CTYRAG | 2 cut(s) | 17, 204 |
| Sse9I | AATT | 3 cut(s) | 197, 249, 318 |
| SsiI | CCGC | 2 cut(s) | 626, 829 |
| SspI | AATATT | 1 cut(s) | 606 |
| SspMI | CTAG | 2 cut(s) | 399, 587 |
| SstI | GAGCTC | 1 cut(s) | 816 |
| StyD4I | CCNGG | 3 cut(s) | 90, 426, 555 |
| TaaI | ACNGT | 2 cut(s) | 104, 372 |
| TaqI | TCGA | 5 cut(s) | 18, 283, 523, 819, 833 |
| TasI | AATT | 3 cut(s) | 197, 249, 318 |
| TfiI | GAWTC | 2 cut(s) | 525, 611 |
| Tru1I | TTAA | 3 cut(s) | 111, 120, 540 |
| Tru9I | TTAA | 3 cut(s) | 111, 120, 540 |
| TseI | GCWGC | 2 cut(s) | 155, 583 |
| TspDTI | ATGAA | 2 cut(s) | 510, 754 |
| TspGWI | ACGGA | 1 cut(s) | 364 |
| VpaK11BI | GGWCC | 1 cut(s) | 652 |
| XapI | RAATTY | 1 cut(s) | 197 |
| XhoI | CTCGAG | 1 cut(s) | 17 |
| XmnI | GAANNNNTTC | 1 cut(s) | 253 |
| XspI | CTAG | 2 cut(s) | 399, 587 |