4-coumarate--CoA ligase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 55 |
| AccI | GTMKAC | 1 cut(s) | 359 |
| AccII | CGCG | 2 cut(s) | 676, 860 |
| AciI | CCGC | 6 cut(s) | 144, 353, 368, 674, 806, 845 |
| AclWI | GGATC | 3 cut(s) | 119, 503, 516 |
| AcoI | YGGCCR | 1 cut(s) | 648 |
| AcsI | RAATTY | 1 cut(s) | 29 |
| AcvI | CACGTG | 1 cut(s) | 682 |
| AcyI | GRCGYC | 2 cut(s) | 56, 362 |
| AfaI | GTAC | 3 cut(s) | 665, 737, 818 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 518 |
| AflIII | ACRYGT | 1 cut(s) | 681 |
| AgeI | ACCGGT | 1 cut(s) | 660 |
| AgsI | TTSAA | 3 cut(s) | 70, 436, 802 |
| AjiI | CACGTC | 2 cut(s) | 403, 553 |
| AluBI | AGCT | 3 cut(s) | 232, 392, 493 |
| AluI | AGCT | 3 cut(s) | 232, 392, 493 |
| Alw26I | GTCTC | 1 cut(s) | 409 |
| AlwI | GGATC | 3 cut(s) | 119, 503, 516 |
| AoxI | GGCC | 2 cut(s) | 261, 648 |
| ApeKI | GCWGC | 2 cut(s) | 200, 562 |
| ApoI | RAATTY | 1 cut(s) | 29 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 228, 260 |
| AsiGI | ACCGGT | 1 cut(s) | 660 |
| AspLEI | GCGC | 4 cut(s) | 58, 562, 678, 862 |
| AspS9I | GGNCC | 1 cut(s) | 658 |
| AsuHPI | GGTGA | 3 cut(s) | 133, 739, 865 |
| AvaII | GGWCC | 1 cut(s) | 658 |
| BamHI | GGATCC | 1 cut(s) | 508 |
| BanI | GGYRCC | 1 cut(s) | 55 |
| BbrPI | CACGTG | 1 cut(s) | 682 |
| BbvI | GCAGC | 2 cut(s) | 187, 549 |
| BccI | CCATC | 4 cut(s) | 116, 328, 634, 766 |
| BceAI | ACGGC | 1 cut(s) | 663 |
| BcoDI | GTCTC | 1 cut(s) | 409 |
| BfoI | RGCGCY | 2 cut(s) | 59, 563 |
| BisI | GCNGC | 4 cut(s) | 201, 353, 563, 674 |
| BlsI | GCNGC | 4 cut(s) | 202, 354, 564, 675 |
| Bme18I | GGWCC | 1 cut(s) | 658 |
| BmgBI | CACGTC | 2 cut(s) | 403, 553 |
| BmgT120I | GGNCC | 1 cut(s) | 658 |
| BmiI | GGNNCC | 3 cut(s) | 57, 510, 843 |
| BmrI | ACTGGG | 1 cut(s) | 594 |
| BmsI | GCATC | 1 cut(s) | 622 |
| BmuI | ACTGGG | 1 cut(s) | 594 |
| BpmI | CTGGAG | 1 cut(s) | 212 |
| BsaAI | YACGTR | 2 cut(s) | 682, 828 |
| BsaBI | GATNNNNATC | 2 cut(s) | 382, 507 |
| BsaHI | GRCGYC | 2 cut(s) | 56, 362 |
| BsaJI | CCNNGG | 1 cut(s) | 51 |
| BsaWI | WCCGGW | 3 cut(s) | 453, 518, 660 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 149, 179 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 518 |
| Bse118I | RCCGGY | 3 cut(s) | 526, 660, 847 |
| Bse1I | ACTGG | 2 cut(s) | 589, 743 |
| Bse8I | GATNNNNATC | 2 cut(s) | 382, 507 |
| BseDI | CCNNGG | 1 cut(s) | 51 |
| BseGI | GGATG | 3 cut(s) | 47, 108, 320 |
| BseJI | GATNNNNATC | 2 cut(s) | 382, 507 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 518 |
| BseMII | CTCAG | 2 cut(s) | 50, 191 |
| BseNI | ACTGG | 2 cut(s) | 589, 743 |
| BseRI | GAGGAG | 2 cut(s) | 154, 157 |
| BseX3I | CGGCCG | 1 cut(s) | 648 |
| BseXI | GCAGC | 2 cut(s) | 187, 549 |
| Bsh1236I | CGCG | 2 cut(s) | 676, 860 |
| Bsh1285I | CGRYCG | 1 cut(s) | 651 |
| BshFI | GGCC | 2 cut(s) | 263, 650 |
| BshNI | GGYRCC | 1 cut(s) | 55 |
| BshTI | ACCGGT | 1 cut(s) | 660 |
| BsiEI | CGRYCG | 1 cut(s) | 651 |
| BsiSI | CCGG | 6 cut(s) | 454, 519, 527, 661, 748, 848 |
| BslFI | GGGAC | 3 cut(s) | 25, 592, 683 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 518 |
| BsmAI | GTCTC | 1 cut(s) | 409 |
| BsmBI | CGTCTC | 1 cut(s) | 409 |
| BsmFI | GGGAC | 3 cut(s) | 25, 592, 683 |
| BsnI | GGCC | 2 cut(s) | 263, 650 |
| Bsp143I | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| Bsp19I | CCATGG | 1 cut(s) | 51 |
| BspACI | CCGC | 6 cut(s) | 144, 353, 368, 674, 806, 845 |
| BspANI | GGCC | 2 cut(s) | 263, 650 |
| BspCNI | CTCAG | 2 cut(s) | 49, 190 |
| BspFNI | CGCG | 2 cut(s) | 676, 860 |
| BspLI | GGNNCC | 3 cut(s) | 57, 510, 843 |
| BspPI | GGATC | 3 cut(s) | 119, 503, 516 |
| BspQI | GCTCTTC | 1 cut(s) | 584 |
| BspT107I | GGYRCC | 1 cut(s) | 55 |
| BsrFI | RCCGGY | 3 cut(s) | 526, 660, 847 |
| BsrI | ACTGG | 2 cut(s) | 589, 743 |
| BssAI | RCCGGY | 3 cut(s) | 526, 660, 847 |
| BssECI | CCNNGG | 1 cut(s) | 51 |
| BssMI | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| BssNI | GRCGYC | 2 cut(s) | 56, 362 |
| BssT1I | CCWWGG | 1 cut(s) | 51 |
| Bst6I | CTCTTC | 3 cut(s) | 158, 331, 584 |
| BstACI | GRCGYC | 2 cut(s) | 56, 362 |
| BstBAI | YACGTR | 2 cut(s) | 682, 828 |
| BstC8I | GCNNGC | 1 cut(s) | 671 |
| BstDEI | CTNAG | 3 cut(s) | 36, 177, 241 |
| BstDSI | CCRYGG | 1 cut(s) | 51 |
| BstF5I | GGATG | 3 cut(s) | 47, 108, 320 |
| BstFNI | CGCG | 2 cut(s) | 676, 860 |
| BstH2I | RGCGCY | 2 cut(s) | 59, 563 |
| BstHHI | GCGC | 4 cut(s) | 58, 562, 678, 862 |
| BstKTI | GATC | 5 cut(s) | 127, 426, 511, 655, 858 |
| BstMAI | GTCTC | 1 cut(s) | 409 |
| BstMBI | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| BstMCI | CGRYCG | 1 cut(s) | 651 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 200, 361, 675, 748, 803, 868 |
| BstSNI | TACGTA | 1 cut(s) | 828 |
| BstUI | CGCG | 2 cut(s) | 676, 860 |
| BstV1I | GCAGC | 2 cut(s) | 187, 549 |
| BstX2I | RGATCY | 1 cut(s) | 508 |
| BstYI | RGATCY | 1 cut(s) | 508 |
| BstZI | CGGCCG | 1 cut(s) | 648 |
| BsuRI | GGCC | 2 cut(s) | 263, 650 |
| BtgI | CCRYGG | 1 cut(s) | 51 |
| BtrI | CACGTC | 2 cut(s) | 403, 553 |
| BtsCI | GGATG | 3 cut(s) | 47, 108, 320 |
| Cac8I | GCNNGC | 1 cut(s) | 671 |
| CfoI | GCGC | 4 cut(s) | 58, 562, 678, 862 |
| Cfr10I | RCCGGY | 3 cut(s) | 526, 660, 847 |
| Cfr13I | GGNCC | 1 cut(s) | 658 |
| CseI | GACGC | 3 cut(s) | 206, 370, 619 |
| Csp6I | GTAC | 3 cut(s) | 664, 736, 817 |
| CspAI | ACCGGT | 1 cut(s) | 660 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 360, 395 |
| CviAII | CATG | 1 cut(s) | 52 |
| CviQI | GTAC | 3 cut(s) | 664, 736, 817 |
| DdeI | CTNAG | 3 cut(s) | 36, 177, 241 |
| DinI | GGCGCC | 1 cut(s) | 57 |
| DpnI | GATC | 5 cut(s) | 126, 425, 510, 654, 857 |
| DpnII | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| EaeI | YGGCCR | 1 cut(s) | 648 |
| EagI | CGGCCG | 1 cut(s) | 648 |
| Eam1104I | CTCTTC | 3 cut(s) | 158, 331, 584 |
| EarI | CTCTTC | 3 cut(s) | 158, 331, 584 |
| EciI | GGCGGA | 1 cut(s) | 834 |
| EclXI | CGGCCG | 1 cut(s) | 648 |
| Eco105I | TACGTA | 1 cut(s) | 828 |
| Eco130I | CCWWGG | 1 cut(s) | 51 |
| Eco147I | AGGCCT | 1 cut(s) | 263 |
| Eco47I | GGWCC | 1 cut(s) | 658 |
| Eco52I | CGGCCG | 1 cut(s) | 648 |
| Eco72I | CACGTG | 1 cut(s) | 682 |
| EcoT14I | CCWWGG | 1 cut(s) | 51 |
| EgeI | GGCGCC | 1 cut(s) | 57 |
| EheI | GGCGCC | 1 cut(s) | 57 |
| ErhI | CCWWGG | 1 cut(s) | 51 |
| Esp3I | CGTCTC | 1 cut(s) | 409 |
| FaeI | CATG | 1 cut(s) | 55 |
| FaiI | YATR | 5 cut(s) | 12, 16, 53, 461, 608 |
| FaqI | GGGAC | 3 cut(s) | 25, 592, 683 |
| FatI | CATG | 1 cut(s) | 51 |
| FblI | GTMKAC | 1 cut(s) | 359 |
| Fnu4HI | GCNGC | 4 cut(s) | 201, 353, 563, 674 |
| FokI | GGATG | 3 cut(s) | 34, 95, 307 |
| Fsp4HI | GCNGC | 4 cut(s) | 201, 353, 563, 674 |
| GlaI | GCGC | 4 cut(s) | 57, 561, 677, 861 |
| GluI | GCNGC | 4 cut(s) | 201, 353, 563, 674 |
| GsuI | CTGGAG | 1 cut(s) | 212 |
| HaeII | RGCGCY | 2 cut(s) | 59, 563 |
| HaeIII | GGCC | 2 cut(s) | 263, 650 |
| HapII | CCGG | 6 cut(s) | 454, 519, 527, 661, 748, 848 |
| HgaI | GACGC | 3 cut(s) | 206, 370, 619 |
| HhaI | GCGC | 4 cut(s) | 58, 562, 678, 862 |
| Hin1I | GRCGYC | 2 cut(s) | 56, 362 |
| Hin1II | CATG | 1 cut(s) | 55 |
| Hin6I | GCGC | 4 cut(s) | 56, 560, 676, 860 |
| HinP1I | GCGC | 4 cut(s) | 56, 560, 676, 860 |
| HincII | GTYRAC | 1 cut(s) | 360 |
| HindII | GTYRAC | 1 cut(s) | 360 |
| HindIII | AAGCTT | 1 cut(s) | 491 |
| HinfI | GANTC | 2 cut(s) | 503, 541 |
| HpaII | CCGG | 6 cut(s) | 454, 519, 527, 661, 748, 848 |
| HphI | GGTGA | 3 cut(s) | 133, 739, 865 |
| Hpy166II | GTNNAC | 2 cut(s) | 360, 524 |
| Hpy188I | TCNGA | 9 cut(s) | 180, 388, 409, 481, 499, 508, 513, 546, 618 |
| Hpy188III | TCNNGA | 4 cut(s) | 283, 577, 656, 700 |
| Hpy8I | GTNNAC | 2 cut(s) | 360, 524 |
| Hpy99I | CGWCG | 5 cut(s) | 200, 212, 298, 364, 637 |
| HpyAV | CCTTC | 6 cut(s) | 76, 124, 232, 274, 484, 796 |
| HpyCH4IV | ACGT | 7 cut(s) | 279, 293, 402, 552, 632, 681, 827 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 203, 382 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 200, 361, 675, 748, 803, 868 |
| HpyF3I | CTNAG | 3 cut(s) | 36, 177, 241 |
| HpySE526I | ACGT | 7 cut(s) | 279, 293, 402, 552, 632, 681, 827 |
| Hsp92I | GRCGYC | 2 cut(s) | 56, 362 |
| Hsp92II | CATG | 1 cut(s) | 55 |
| HspAI | GCGC | 4 cut(s) | 56, 560, 676, 860 |
| KasI | GGCGCC | 1 cut(s) | 55 |
| Kzo9I | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| LguI | GCTCTTC | 1 cut(s) | 584 |
| Lsp1109I | GCAGC | 2 cut(s) | 187, 549 |
| LweI | GCATC | 1 cut(s) | 622 |
| MaeII | ACGT | 7 cut(s) | 279, 293, 402, 552, 632, 681, 827 |
| MaeIII | GTNAC | 4 cut(s) | 548, 643, 766, 823 |
| MalI | GATC | 5 cut(s) | 126, 425, 510, 654, 857 |
| MboI | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| MboII | GAAGA | 6 cut(s) | 145, 297, 318, 333, 433, 601 |
| MflI | RGATCY | 1 cut(s) | 508 |
| MluCI | AATT | 3 cut(s) | 29, 315, 444 |
| Mly113I | GGCGCC | 1 cut(s) | 56 |
| MseI | TTAA | 2 cut(s) | 489, 534 |
| MspI | CCGG | 6 cut(s) | 454, 519, 527, 661, 748, 848 |
| MvnI | CGCG | 2 cut(s) | 676, 860 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 200, 361, 675, 748, 803, 868 |
| NarI | GGCGCC | 1 cut(s) | 56 |
| NcoI | CCATGG | 1 cut(s) | 51 |
| NdeII | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| NlaIII | CATG | 1 cut(s) | 55 |
| NlaIV | GGNNCC | 3 cut(s) | 57, 510, 843 |
| NmuCI | GTSAC | 2 cut(s) | 548, 643 |
| PceI | AGGCCT | 1 cut(s) | 263 |
| PciSI | GCTCTTC | 1 cut(s) | 584 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 192, 204, 356, 578 |
| PfeI | GAWTC | 2 cut(s) | 503, 541 |
| PinAI | ACCGGT | 1 cut(s) | 660 |
| PkrI | GCNGC | 4 cut(s) | 202, 354, 564, 675 |
| PluTI | GGCGCC | 1 cut(s) | 59 |
| PmaCI | CACGTG | 1 cut(s) | 682 |
| PmlI | CACGTG | 1 cut(s) | 682 |
| Ppu21I | YACGTR | 2 cut(s) | 682, 828 |
| PspCI | CACGTG | 1 cut(s) | 682 |
| PspN4I | GGNNCC | 3 cut(s) | 57, 510, 843 |
| PspPI | GGNCC | 1 cut(s) | 658 |
| PsuI | RGATCY | 1 cut(s) | 508 |
| RsaI | GTAC | 3 cut(s) | 665, 737, 818 |
| RsaNI | GTAC | 3 cut(s) | 664, 736, 817 |
| SalI | GTCGAC | 1 cut(s) | 358 |
| SapI | GCTCTTC | 1 cut(s) | 584 |
| SaqAI | TTAA | 2 cut(s) | 489, 534 |
| SatI | GCNGC | 4 cut(s) | 201, 353, 563, 674 |
| Sau3AI | GATC | 5 cut(s) | 124, 423, 508, 652, 855 |
| Sau96I | GGNCC | 1 cut(s) | 658 |
| SfaNI | GCATC | 1 cut(s) | 622 |
| SfoI | GGCGCC | 1 cut(s) | 57 |
| SgrAI | CRCCGGYG | 1 cut(s) | 847 |
| SinI | GGWCC | 1 cut(s) | 658 |
| SnaBI | TACGTA | 1 cut(s) | 828 |
| Sse9I | AATT | 3 cut(s) | 29, 315, 444 |
| SseBI | AGGCCT | 1 cut(s) | 263 |
| SsiI | CCGC | 6 cut(s) | 144, 353, 368, 674, 806, 845 |
| SspDI | GGCGCC | 1 cut(s) | 55 |
| StuI | AGGCCT | 1 cut(s) | 263 |
| StyI | CCWWGG | 1 cut(s) | 51 |
| TaiI | ACGT | 7 cut(s) | 282, 296, 405, 555, 635, 684, 830 |
| TaqI | TCGA | 4 cut(s) | 359, 426, 448, 789 |
| TasI | AATT | 3 cut(s) | 29, 315, 444 |
| TatI | WGTACW | 1 cut(s) | 735 |
| TauI | GCSGC | 2 cut(s) | 355, 676 |
| TfiI | GAWTC | 2 cut(s) | 503, 541 |
| Tru1I | TTAA | 2 cut(s) | 489, 534 |
| Tru9I | TTAA | 2 cut(s) | 489, 534 |
| TseFI | GTSAC | 2 cut(s) | 548, 643 |
| TseI | GCWGC | 2 cut(s) | 200, 562 |
| Tsp45I | GTSAC | 2 cut(s) | 548, 643 |
| TspDTI | ATGAA | 2 cut(s) | 476, 613 |
| VpaK11BI | GGWCC | 1 cut(s) | 658 |
| XapI | RAATTY | 1 cut(s) | 29 |
| XmiI | GTMKAC | 1 cut(s) | 359 |