Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000517

Physical Location & Seq

Genomic Coordinates Reverse (-)

13691238 .. 13695365

Gene Structure 4128 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1145900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGGAGAAATCTGGGTTCGGCAGAGATGGCATTTTCCGGTCACTTCGGCCGCCGCTTGTCCTACCCAGAGACCCAAATCTCTCAATGGTCTCCTTCCTCTTCAGAAACTCCTCCTACTCCCATAAGCCCGCGCTCATCGACGGCGAGTCCTCCGAAACCCTGTCCTTCTCGCAGTTCAAGTCCAAAGTTATCCAGGTCTCCCATGGCTTAATCAATCTGGGTATCAAGAAAAACGACGTCGTCCTCATTTTCGCCCCCAACTCAATCCAATTCCCCATTTGTCTCTTCGGGATTATTGCGTCCGGCGCTATTGCCACCACCTCCAATCCGCTCTACACTGTTTCAGAGCTGTCCAAGCAAGTCCGGGACTCTAATCCCAAGCTGGTTATAACAGTCCCGGAGCTCTGGGATAAAGTCAAGGGCTTTAACCTCCCAACAGTGTTCTTAGGATCAACGGGTTTTTCGGGGGAATCCCACGTTGGATCAGCCTCAAAGATTCGAACTTTCCATGATTTGATCGAGTCTGCTGGATCTGTGTCCGATTTTCCATCGGTTAACATTAAGCAGACCGACACAGCTGTCCTGTTGTACTCTTCAGGTACAACAGGGGTCAGCAAAGGGGTAGTTTTGACACATAGGAATTTCATTGCCTCGTCTCTGATGGTCACAATGGATCAAGAACTTTCTGGGGAGATGCACTATGTGTTTCTTTGTGTTCTACCAATGTTCCATGTGTTTGGGCTGGCAATTATCACCTATGGGCAGCTGCAGAAGGGCAATGCTGTGGTCTCGATGGGGCGATTCGATCTCGAGAAGGTTTTGATGGCCGTTGAGAAGTATAGGGTGACACACCTGTGGATTGTGCCTCCTATAGTGCTTGCTCTGGCGAAAAACAGCGTGGTTAAGAAGTTTAACCTTTCGTCGTTGAAGCATATCGGTTCAGGGGCAGCTCCGTTGGGGAAAGAGTTGATGGAGGAGTGTGCTAAAATTATTCCTCAAGGTGTAGTAGCTCAGGGCTATGGTATGACAGAAACTTGTGGAATTGTTTCGGTGGAGAACACACTAGCGGGGTCTCGACATACTGGTTCAACTGGCATGCTTGTTTCTGGAGTTGAATGTCAGATAGTCAGCGTAGATACACTGAAGCCTCAACCTCCCAAACAGTTGGGAGAGATATGGGTTAGGGGGCCTAATATGATGACTGGGTATTTCAACAACCCACAGGCCACCAACGAGACTATAGATAAAAATGGTTGGGTACACACAGGAGATCTTGGATACATTGATGAAGACGGACAACTTTATGTAGTTGATCGAATCAAAGAGCTCATTAAGTATAAAGGTTTCCAGGTGGCACCAGCTGAACTTGAAGGGCTGCTAGTTTCTCACCCTGAAATATTAGATGCGGTTGTCATCCCGTTCCCTGATGCTGAAGCTGGTGAGGTCCCCGCTGCATATTGTGTACGTACGCCAAATAGTTCTCTAACTGAAGAAGATGTCAAGAGCTTTATTGCTAGTCAAGTTGCATCTTTCAAACGACTACGAAGAGTATCATTCATAAACAGTGTCCCCAAGTCAGCATCGGGCAAAATCCTCAGAAGAGAGCTCATCGAGAAAGTGCGGTCGAAAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004321 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko01100 ko01110 map00130 map00360 map00940 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

545

Amino Acids

59.24

Weight (kDa)

8.54

Isoelectric Point (pI)

31.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	38 - 404	1.6e-85	AMP-binding enzyme
AMP-binding_C	PF13193	455 - 530	9.8e-15	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000517)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05160
fragaria_vesca	FvH4_3g30980 FvH4_3g30980 FvH4_3g30980 FvH4_3g30980 FvH4_3g30980 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460 FvH4_6g16460
malus_domestica	MD00G1033000.v1.1 MD00G1033100.v1.1 MD11G1145900.v1.1
prunus_persica	Prupe.1G562500_v2.0.a1 Prupe.6G109000_v2.0.a1 Prupe.6G220600_v2.0.a1 Prupe.7G129300_v2.0.a1
pyrus_communis	pycom10g19750 pycom11g11900
rosa_chinensis	RchiOBHm_Chr4g0437371 RchiOBHm_Chr5g0057221 RchiOBHm_Chr5g0057231 RchiOBHm_Chr5g0057411 RchiOBHm_Chr5g0057421 RchiOBHm_Chr5g0057431 RchiOBHm_Chr5g0057541 RchiOBHm_Chr5g0057621 RchiOBHm_Chr5g0057631 RchiOBHm_Chr7g0218731
rosa_laevigata	RLG00000024275 RLG00000035153 RLG00000035162 RLG00000035163
rosa_multiflora	Rmu_co8225990.1_g000001 Rmu_co8293931.1_g000001 Rmu_co8305349.1_g000001 Rmu_sc0001058.1_g000006 Rmu_sc0001334.1_g000005 Rmu_sc0002486.1_g000002 Rmu_sc0002486.1_g000004 Rmu_sc0002486.1_g000006 Rmu_sc0003730.1_g000007 Rmu_sc0009763.1_g000003 Rmu_sc0012503.1_g000004 Rmu_sc0041005.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022890 Rroxscaffold_1G00022900 Rroxscaffold_1G00023020 Rroxscaffold_1G00039850 Rroxscaffold_2G00089660 Rroxscaffold_6G00410920 Rroxscaffold_7G00210330
rosa_rugosa	Rorug01G0121100 Rorug05G0306100 Rorug05G0306200 Rorug05G0306700 Rorug05G0308100
rosa_samantha	Rh1AG142700 Rh1BG111300 Rh1DG148300 Rh2DG519700 Rh3DG192200 Rh5BG387400 Rh5BG387600 Rh5CG409800 Rh5CG409900 Rh5CG410000 Rh5CG411000 Rh5CG411600 Rh5CG411700 Rh5DG400500 Rh5DG400600 Rh5DG400700 Rh5DG401700 Rh5DG401800 Rh7CG338500
rosa_wichuraiana	Rw5G035330 Rw5G035540 Rw5G035550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	389
AasI	GACNNNNNNGTC	1 cut(s)	578
AatII	GACGTC	1 cut(s)	240
AccB1I	GGYRCC	1 cut(s)	1354
AccBSI	CCGCTC	1 cut(s)	331
AccII	CGCG	1 cut(s)	131
AciI	CCGC	8 cut(s)	50, 53, 129, 329, 1067, 1406, 1449, 1621
AclWI	GGATC	4 cut(s)	457, 490, 538, 681
AcoI	YGGCCR	2 cut(s)	47, 825
AcsI	RAATTY	1 cut(s)	640
AcuI	CTGAAG	5 cut(s)	85, 579, 1163, 1452, 1509
AcyI	GRCGYC	1 cut(s)	237
AdeI	CACNNNGTG	1 cut(s)	703
AfaI	GTAC	5 cut(s)	590, 601, 1260, 1464, 1468
AgsI	TTSAA	7 cut(s)	178, 928, 1089, 1115, 1213, 1370, 1534
AhdI	GACNNNNNGTC	1 cut(s)	145
AjnI	CCWGG	2 cut(s)	192, 1347
AjuI	GAANNNNNNNTTGG	2 cut(s)	462, 494
AleI	CACNNNNGTG	1 cut(s)	853
Alw21I	GWGCWC	3 cut(s)	405, 1329, 1608
Alw26I	GTCTC	8 cut(s)	63, 94, 202, 287, 660, 793, 1077, 1229
AlwI	GGATC	4 cut(s)	457, 490, 538, 681
Ama87I	CYCGRG	1 cut(s)	809
AoxI	GGCC	4 cut(s)	47, 825, 1187, 1224
ApeKI	GCWGC	5 cut(s)	763, 766, 947, 1375, 1451
ApoI	RAATTY	1 cut(s)	640
Asp700I	GAANNNNTTC	1 cut(s)	1045
AspLEI	GCGC	2 cut(s)	133, 308
AspS9I	GGNCC	2 cut(s)	1187, 1444
AsuC2I	CCSGG	2 cut(s)	365, 398
AsuHPI	GGTGA	4 cut(s)	745, 856, 1379, 1451
AsuII	TTCGAA	1 cut(s)	499
AvaI	CYCGRG	1 cut(s)	809
AvaII	GGWCC	1 cut(s)	1444
BanI	GGYRCC	1 cut(s)	1354
BanII	GRGCYC	3 cut(s)	405, 1329, 1608
BbsI	GAAGAC	1 cut(s)	1296
Bbv12I	GWGCWC	3 cut(s)	405, 1329, 1608
BbvI	GCAGC	5 cut(s)	753, 775, 959, 1362, 1438
BccI	CCATC	6 cut(s)	20, 556, 655, 787, 817, 964
BceAI	ACGGC	2 cut(s)	157, 812
BciT130I	CCWGG	2 cut(s)	194, 1349
BciVI	GTATCC	1 cut(s)	1271
BcnI	CCSGG	2 cut(s)	365, 398
BcoDI	GTCTC	8 cut(s)	63, 94, 202, 287, 660, 793, 1077, 1229
BfaI	CTAG	3 cut(s)	1064, 1379, 1515
BfmI	CTRYAG	3 cut(s)	767, 870, 1239
BfoI	RGCGCY	1 cut(s)	309
BfuI	GTATCC	1 cut(s)	1271
BglII	AGATCT	1 cut(s)	1270
BisI	GCNGC	7 cut(s)	50, 53, 764, 767, 948, 1376, 1452
BlsI	GCNGC	7 cut(s)	51, 54, 765, 768, 949, 1377, 1453
Bme1390I	CCNGG	4 cut(s)	194, 365, 398, 1349
Bme18I	GGWCC	1 cut(s)	1444
BmeRI	GACNNNNNGTC	1 cut(s)	145
BmeT110I	CYCGRG	1 cut(s)	809
BmgT120I	GGNCC	2 cut(s)	1187, 1444
BmiI	GGNNCC	3 cut(s)	1188, 1356, 1446
BmrFI	CCNGG	4 cut(s)	194, 365, 398, 1349
BmrI	ACTGGG	1 cut(s)	1212
BmsI	GCATC	5 cut(s)	684, 1393, 1417, 1535, 1589
BmuI	ACTGGG	1 cut(s)	1212
BpiI	GAAGAC	1 cut(s)	1296
BpmI	CTGGAG	1 cut(s)	1128
Bpu10I	CCTNAGC	1 cut(s)	1011
Bpu14I	TTCGAA	1 cut(s)	499
BpuEI	CTTGAG	1 cut(s)	981
BpuMI	CCSGG	2 cut(s)	365, 398
BsaAI	YACGTR	1 cut(s)	1466
BsaHI	GRCGYC	1 cut(s)	237
BsaI	GGTCTC	5 cut(s)	63, 94, 202, 793, 1077
BsaJI	CCNNGG	1 cut(s)	202
BsaWI	WCCGGW	1 cut(s)	36
Bse1I	ACTGG	3 cut(s)	1087, 1096, 1207
Bse3DI	GCAATG	2 cut(s)	645, 784
BseBI	CCWGG	2 cut(s)	194, 1349
BseDI	CCNNGG	1 cut(s)	202
BseGI	GGATG	1 cut(s)	1413
BseMI	GCAATG	2 cut(s)	645, 784
BseMII	CTCAG	2 cut(s)	1025, 1609
BseNI	ACTGG	3 cut(s)	1087, 1096, 1207
BseRI	GAGGAG	2 cut(s)	100, 989
BseX3I	CGGCCG	1 cut(s)	47
BseXI	GCAGC	5 cut(s)	753, 775, 959, 1362, 1438
Bsh1236I	CGCG	1 cut(s)	131
Bsh1285I	CGRYCG	2 cut(s)	50, 1625
BshFI	GGCC	4 cut(s)	49, 827, 1189, 1226
BshNI	GGYRCC	1 cut(s)	1354
BsiEI	CGRYCG	2 cut(s)	50, 1625
BsiHKAI	GWGCWC	3 cut(s)	405, 1329, 1608
BsiHKCI	CYCGRG	1 cut(s)	809
BsiSI	CCGG	4 cut(s)	37, 303, 364, 398
BsiWI	CGTACG	1 cut(s)	1466
BslFI	GGGAC	4 cut(s)	380, 380, 1430, 1553
BsmAI	GTCTC	8 cut(s)	63, 94, 202, 287, 660, 793, 1077, 1229
BsmBI	CGTCTC	1 cut(s)	660
BsmFI	GGGAC	4 cut(s)	380, 380, 1430, 1553
BsnI	GGCC	4 cut(s)	49, 827, 1189, 1226
Bso31I	GGTCTC	5 cut(s)	63, 94, 202, 793, 1077
BsoBI	CYCGRG	1 cut(s)	809
Bsp119I	TTCGAA	1 cut(s)	499
Bsp1286I	GDGCHC	3 cut(s)	405, 1329, 1608
Bsp143I	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
Bsp19I	CCATGG	1 cut(s)	202
BspACI	CCGC	8 cut(s)	50, 53, 129, 329, 1067, 1406, 1449, 1621
BspANI	GGCC	4 cut(s)	49, 827, 1189, 1226
BspCNI	CTCAG	2 cut(s)	1024, 1608
BspFNI	CGCG	1 cut(s)	131
BspLI	GGNNCC	3 cut(s)	1188, 1356, 1446
BspMAI	CTGCAG	1 cut(s)	771
BspPI	GGATC	4 cut(s)	457, 490, 538, 681
BspT104I	TTCGAA	1 cut(s)	499
BspT107I	GGYRCC	1 cut(s)	1354
BspTNI	GGTCTC	5 cut(s)	63, 94, 202, 793, 1077
BsrBI	CCGCTC	1 cut(s)	331
BsrDI	GCAATG	2 cut(s)	645, 784
BsrI	ACTGG	3 cut(s)	1087, 1096, 1207
BssECI	CCNNGG	1 cut(s)	202
BssMI	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
BssNI	GRCGYC	1 cut(s)	237
BssT1I	CCWWGG	1 cut(s)	202
Bst2UI	CCWGG	2 cut(s)	194, 1349
Bst4CI	ACNGT	5 cut(s)	340, 394, 439, 1164, 1565
Bst6I	CTCTTC	5 cut(s)	104, 290, 598, 1540, 1594
BstACI	GRCGYC	1 cut(s)	237
BstBAI	YACGTR	1 cut(s)	1466
BstBI	TTCGAA	1 cut(s)	499
BstC8I	GCNNGC	4 cut(s)	129, 744, 879, 1097
BstDEI	CTNAG	3 cut(s)	445, 1011, 1595
BstDSI	CCRYGG	1 cut(s)	202
BstF5I	GGATG	1 cut(s)	1413
BstFNI	CGCG	1 cut(s)	131
BstH2I	RGCGCY	1 cut(s)	309
BstHHI	GCGC	2 cut(s)	133, 308
BstKTI	GATC	8 cut(s)	452, 485, 519, 533, 676, 808, 1273, 1315
BstMAI	GTCTC	8 cut(s)	63, 94, 202, 287, 660, 793, 1077, 1229
BstMBI	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
BstMCI	CGRYCG	2 cut(s)	50, 1625
BstMWI	GCNNNNNNNGC	3 cut(s)	27, 305, 355
BstNI	CCWGG	2 cut(s)	194, 1349
BstNSI	RCATGY	1 cut(s)	1099
BstSCI	CCNGG	4 cut(s)	192, 363, 396, 1347
BstSFI	CTRYAG	3 cut(s)	767, 870, 1239
BstSNI	TACGTA	1 cut(s)	1466
BstUI	CGCG	1 cut(s)	131
BstV1I	GCAGC	5 cut(s)	753, 775, 959, 1362, 1438
BstV2I	GAAGAC	1 cut(s)	1296
BstX2I	RGATCY	2 cut(s)	530, 1270
BstXI	CCANNNNNNTGG	2 cut(s)	737, 1165
BstYI	RGATCY	2 cut(s)	530, 1270
BstZI	CGGCCG	1 cut(s)	47
BsuI	GTATCC	1 cut(s)	1271
BsuRI	GGCC	4 cut(s)	49, 827, 1189, 1226
BtgI	CCRYGG	1 cut(s)	202
BtsCI	GGATG	1 cut(s)	1413
BtsIMutI	CAGTG	4 cut(s)	336, 444, 1139, 1570
Cac8I	GCNNGC	4 cut(s)	129, 744, 879, 1097
CfoI	GCGC	2 cut(s)	133, 308
Cfr13I	GGNCC	2 cut(s)	1187, 1444
CseI	GACGC	1 cut(s)	288
Csp6I	GTAC	5 cut(s)	589, 600, 1259, 1463, 1467
CviAII	CATG	4 cut(s)	203, 509, 731, 1096
CviQI	GTAC	5 cut(s)	589, 600, 1259, 1463, 1467
DdeI	CTNAG	3 cut(s)	445, 1011, 1595
DpnI	GATC	8 cut(s)	451, 484, 518, 532, 675, 807, 1272, 1314
DpnII	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
DraIII	CACNNNGTG	1 cut(s)	703
DrdI	GACNNNNNNGTC	1 cut(s)	578
DriI	GACNNNNNGTC	1 cut(s)	145
DseDI	GACNNNNNNGTC	1 cut(s)	578
EaeI	YGGCCR	2 cut(s)	47, 825
EagI	CGGCCG	1 cut(s)	47
Eam1104I	CTCTTC	5 cut(s)	104, 290, 598, 1540, 1594
Eam1105I	GACNNNNNGTC	1 cut(s)	145
EarI	CTCTTC	5 cut(s)	104, 290, 598, 1540, 1594
Ecl136II	GAGCTC	3 cut(s)	403, 1327, 1606
EclXI	CGGCCG	1 cut(s)	47
Eco105I	TACGTA	1 cut(s)	1466
Eco130I	CCWWGG	1 cut(s)	202
Eco24I	GRGCYC	3 cut(s)	405, 1329, 1608
Eco31I	GGTCTC	5 cut(s)	63, 94, 202, 793, 1077
Eco47I	GGWCC	1 cut(s)	1444
Eco52I	CGGCCG	1 cut(s)	47
Eco53kI	GAGCTC	3 cut(s)	403, 1327, 1606
Eco57I	CTGAAG	5 cut(s)	85, 579, 1163, 1452, 1509
Eco88I	CYCGRG	1 cut(s)	809
EcoICRI	GAGCTC	3 cut(s)	403, 1327, 1606
EcoO109I	RGGNCCY	2 cut(s)	1187, 1444
EcoRII	CCWGG	2 cut(s)	192, 1347
EcoT14I	CCWWGG	1 cut(s)	202
EcoT38I	GRGCYC	3 cut(s)	405, 1329, 1608
ErhI	CCWWGG	1 cut(s)	202
Esp3I	CGTCTC	1 cut(s)	660
FaeI	CATG	4 cut(s)	206, 512, 734, 1099
FaqI	GGGAC	4 cut(s)	380, 380, 1430, 1553
FatI	CATG	4 cut(s)	202, 508, 730, 1095
FauI	CCCGC	3 cut(s)	136, 1060, 1456
Fnu4HI	GCNGC	7 cut(s)	50, 53, 764, 767, 948, 1376, 1452
FokI	GGATG	1 cut(s)	1400
FriOI	GRGCYC	3 cut(s)	405, 1329, 1608
Fsp4HI	GCNGC	7 cut(s)	50, 53, 764, 767, 948, 1376, 1452
FspBI	CTAG	3 cut(s)	1064, 1379, 1515
GlaI	GCGC	2 cut(s)	132, 307
GluI	GCNGC	7 cut(s)	50, 53, 764, 767, 948, 1376, 1452
GsuI	CTGGAG	1 cut(s)	1128
HaeII	RGCGCY	1 cut(s)	309
HaeIII	GGCC	4 cut(s)	49, 827, 1189, 1226
HapII	CCGG	4 cut(s)	37, 303, 364, 398
HgaI	GACGC	1 cut(s)	288
HhaI	GCGC	2 cut(s)	133, 308
Hin1I	GRCGYC	1 cut(s)	237
Hin1II	CATG	4 cut(s)	206, 512, 734, 1099
Hin6I	GCGC	2 cut(s)	131, 306
HinP1I	GCGC	2 cut(s)	131, 306
HincII	GTYRAC	1 cut(s)	556
HindII	GTYRAC	1 cut(s)	556
HinfI	GANTC	7 cut(s)	146, 368, 470, 496, 521, 801, 1317
HpaI	GTTAAC	1 cut(s)	556
HpaII	CCGG	4 cut(s)	37, 303, 364, 398
HphI	GGTGA	4 cut(s)	745, 856, 1379, 1451
Hpy166II	GTNNAC	3 cut(s)	556, 1261, 1463
Hpy188I	TCNGA	7 cut(s)	104, 154, 346, 541, 660, 1122, 1598
Hpy8I	GTNNAC	3 cut(s)	556, 1261, 1463
Hpy99I	CGWCG	4 cut(s)	143, 239, 242, 925
HpyAV	CCTTC	5 cut(s)	103, 175, 766, 808, 1364
HpyCH4III	ACNGT	5 cut(s)	340, 394, 439, 1164, 1565
HpyCH4IV	ACGT	3 cut(s)	237, 477, 1465
HpyCH4V	TGCA	4 cut(s)	697, 769, 1454, 1526
HpyF10VI	GCNNNNNNNGC	3 cut(s)	27, 305, 355
HpyF3I	CTNAG	3 cut(s)	445, 1011, 1595
HpySE526I	ACGT	3 cut(s)	237, 477, 1465
Hsp92I	GRCGYC	1 cut(s)	237
Hsp92II	CATG	4 cut(s)	206, 512, 734, 1099
HspAI	GCGC	2 cut(s)	131, 306
KspAI	GTTAAC	1 cut(s)	556
Kzo9I	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
LmnI	GCTCC	2 cut(s)	400, 955
Lsp1109I	GCAGC	5 cut(s)	753, 775, 959, 1362, 1438
LweI	GCATC	5 cut(s)	684, 1393, 1417, 1535, 1589
MaeI	CTAG	3 cut(s)	1064, 1379, 1515
MaeII	ACGT	3 cut(s)	237, 477, 1465
MaeIII	GTNAC	3 cut(s)	39, 664, 844
MalI	GATC	8 cut(s)	451, 484, 518, 532, 675, 807, 1272, 1314
MbiI	CCGCTC	1 cut(s)	331
MboI	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
MboII	GAAGA	8 cut(s)	91, 277, 585, 1301, 1502, 1505, 1557, 1611
MflI	RGATCY	2 cut(s)	530, 1270
MhlI	GDGCHC	3 cut(s)	405, 1329, 1608
MluCI	AATT	5 cut(s)	269, 640, 747, 987, 1041
MlyI	GAGTC	3 cut(s)	155, 362, 530
MmeI	TCCRAC	1 cut(s)	460
MroXI	GAANNNNTTC	1 cut(s)	1045
MseI	TTAA	7 cut(s)	209, 426, 555, 561, 903, 912, 1332
MslI	CAYNNNNRTG	1 cut(s)	853
MspA1I	CMGCKG	4 cut(s)	578, 766, 1361, 1451
MspI	CCGG	4 cut(s)	37, 303, 364, 398
MspR9I	CCNGG	4 cut(s)	194, 365, 398, 1349
MvaI	CCWGG	2 cut(s)	194, 1349
MvnI	CGCG	1 cut(s)	131
MwoI	GCNNNNNNNGC	3 cut(s)	27, 305, 355
NciI	CCSGG	2 cut(s)	365, 398
NcoI	CCATGG	1 cut(s)	202
NdeII	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
NlaIII	CATG	4 cut(s)	206, 512, 734, 1099
NlaIV	GGNNCC	3 cut(s)	1188, 1356, 1446
NmuCI	GTSAC	3 cut(s)	39, 664, 844
NspI	RCATGY	1 cut(s)	1099
NspV	TTCGAA	1 cut(s)	499
OliI	CACNNNNGTG	1 cut(s)	853
PaeI	GCATGC	1 cut(s)	1099
PaeR7I	CTCGAG	1 cut(s)	809
PdmI	GAANNNNTTC	1 cut(s)	1045
PfeI	GAWTC	4 cut(s)	470, 496, 801, 1317
Pfl23II	CGTACG	1 cut(s)	1466
PflFI	GACNNNGTC	1 cut(s)	239
PfoI	TCCNGGA	2 cut(s)	363, 396
PkrI	GCNGC	7 cut(s)	51, 54, 765, 768, 949, 1377, 1453
PleI	GAGTC	3 cut(s)	154, 362, 529
PpsI	GAGTC	3 cut(s)	154, 362, 529
Ppu21I	YACGTR	1 cut(s)	1466
PpuMI	RGGWCCY	1 cut(s)	1444
PsiI	TTATAA	1 cut(s)	389
Psp124BI	GAGCTC	3 cut(s)	405, 1329, 1608
Psp5II	RGGWCCY	1 cut(s)	1444
Psp6I	CCWGG	2 cut(s)	192, 1347
PspGI	CCWGG	2 cut(s)	192, 1347
PspLI	CGTACG	1 cut(s)	1466
PspN4I	GGNNCC	3 cut(s)	1188, 1356, 1446
PspPI	GGNCC	2 cut(s)	1187, 1444
PspPPI	RGGWCCY	1 cut(s)	1444
PstI	CTGCAG	1 cut(s)	771
PsuI	RGATCY	2 cut(s)	530, 1270
PsyI	GACNNNGTC	1 cut(s)	239
PvuII	CAGCTG	3 cut(s)	578, 766, 1361
RsaI	GTAC	5 cut(s)	590, 601, 1260, 1464, 1468
RsaNI	GTAC	5 cut(s)	589, 600, 1259, 1463, 1467
RseI	CAYNNNNRTG	1 cut(s)	853
SacI	GAGCTC	3 cut(s)	405, 1329, 1608
SaqAI	TTAA	7 cut(s)	209, 426, 555, 561, 903, 912, 1332
SatI	GCNGC	7 cut(s)	50, 53, 764, 767, 948, 1376, 1452
Sau3AI	GATC	8 cut(s)	449, 482, 516, 530, 673, 805, 1270, 1312
Sau96I	GGNCC	2 cut(s)	1187, 1444
SchI	GAGTC	3 cut(s)	155, 362, 530
ScrFI	CCNGG	4 cut(s)	194, 365, 398, 1349
SduI	GDGCHC	3 cut(s)	405, 1329, 1608
SfaNI	GCATC	5 cut(s)	684, 1393, 1417, 1535, 1589
SfcI	CTRYAG	3 cut(s)	767, 870, 1239
Sfr274I	CTCGAG	1 cut(s)	809
SfuI	TTCGAA	1 cut(s)	499
SinI	GGWCC	1 cut(s)	1444
SlaI	CTCGAG	1 cut(s)	809
SmiMI	CAYNNNNRTG	1 cut(s)	853
SmlI	CTYRAG	2 cut(s)	809, 996
SmoI	CTYRAG	2 cut(s)	809, 996
SnaBI	TACGTA	1 cut(s)	1466
SphI	GCATGC	1 cut(s)	1099
Sse9I	AATT	5 cut(s)	269, 640, 747, 987, 1041
SsiI	CCGC	8 cut(s)	50, 53, 129, 329, 1067, 1406, 1449, 1621
SspI	AATATT	1 cut(s)	1398
SspMI	CTAG	3 cut(s)	1064, 1379, 1515
SstI	GAGCTC	3 cut(s)	405, 1329, 1608
StyD4I	CCNGG	4 cut(s)	192, 363, 396, 1347
StyI	CCWWGG	1 cut(s)	202
TaaI	ACNGT	5 cut(s)	340, 394, 439, 1164, 1565
TaiI	ACGT	3 cut(s)	240, 480, 1468
TaqII	GACCGA	1 cut(s)	584
TasI	AATT	5 cut(s)	269, 640, 747, 987, 1041
TatI	WGTACW	1 cut(s)	588
TauI	GCSGC	2 cut(s)	52, 55
TfiI	GAWTC	4 cut(s)	470, 496, 801, 1317
Tru1I	TTAA	7 cut(s)	209, 426, 555, 561, 903, 912, 1332
Tru9I	TTAA	7 cut(s)	209, 426, 555, 561, 903, 912, 1332
TscAI	CASTG	4 cut(s)	343, 444, 1146, 1570
TseFI	GTSAC	3 cut(s)	39, 664, 844
TseI	GCWGC	5 cut(s)	763, 766, 947, 1375, 1451
Tsp45I	GTSAC	3 cut(s)	39, 664, 844
TspDTI	ATGAA	3 cut(s)	634, 1302, 1546
TspGWI	ACGGA	2 cut(s)	942, 1308
TspRI	CASTG	4 cut(s)	343, 444, 1146, 1570
Tth111I	GACNNNGTC	1 cut(s)	239
VpaK11BI	GGWCC	1 cut(s)	1444
XapI	RAATTY	1 cut(s)	640
XceI	RCATGY	1 cut(s)	1099
XcmI	CCANNNNNNNNNTGG	1 cut(s)	200
XhoI	CTCGAG	1 cut(s)	809
XmnI	GAANNNNTTC	1 cut(s)	1045
XspI	CTAG	3 cut(s)	1064, 1379, 1515
ZraI	GACGTC	1 cut(s)	238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.