4-coumarate--CoA ligase-like
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 392 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1406 |
| Acc36I | ACCTGC | 1 cut(s) | 139 |
| AccB1I | GGYRCC | 1 cut(s) | 308 |
| AcoI | YGGCCR | 3 cut(s) | 19, 741, 1291 |
| AcsI | RAATTY | 2 cut(s) | 637, 1054 |
| AcuI | CTGAAG | 2 cut(s) | 19, 1449 |
| AfaI | GTAC | 6 cut(s) | 587, 598, 836, 908, 1257, 1617 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 852, 853, 1573 |
| AjiI | CACGTC | 1 cut(s) | 241 |
| AjnI | CCWGG | 1 cut(s) | 1186 |
| Alw21I | GWGCWC | 3 cut(s) | 155, 880, 1605 |
| Alw26I | GTCTC | 3 cut(s) | 63, 94, 538 |
| AoxI | GGCC | 8 cut(s) | 19, 527, 604, 741, 1184, 1221, 1291, 1369 |
| ApeKI | GCWGC | 4 cut(s) | 575, 760, 763, 944 |
| ApoI | RAATTY | 2 cut(s) | 637, 1054 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1042 |
| AspLEI | GCGC | 2 cut(s) | 580, 1464 |
| AspS9I | GGNCC | 6 cut(s) | 452, 527, 1067, 1184, 1441, 1620 |
| AsuHPI | GGTGA | 5 cut(s) | 73, 430, 835, 1376, 1448 |
| AvaII | GGWCC | 4 cut(s) | 452, 1067, 1441, 1620 |
| BalI | TGGCCA | 1 cut(s) | 1293 |
| BanI | GGYRCC | 1 cut(s) | 308 |
| BanII | GRGCYC | 1 cut(s) | 1605 |
| BbsI | GAAGAC | 1 cut(s) | 25 |
| Bbv12I | GWGCWC | 3 cut(s) | 155, 880, 1605 |
| BbvCI | CCTCAGC | 1 cut(s) | 608 |
| BbvI | GCAGC | 4 cut(s) | 562, 750, 772, 956 |
| BccI | CCATC | 5 cut(s) | 20, 225, 961, 1158, 1192 |
| BceAI | ACGGC | 2 cut(s) | 6, 728 |
| BciT130I | CCWGG | 1 cut(s) | 1188 |
| BciVI | GTATCC | 1 cut(s) | 1268 |
| BcoDI | GTCTC | 3 cut(s) | 63, 94, 538 |
| BfaI | CTAG | 4 cut(s) | 546, 953, 1061, 1376 |
| BfuAI | ACCTGC | 1 cut(s) | 139 |
| BfuI | GTATCC | 1 cut(s) | 1268 |
| BglII | AGATCT | 1 cut(s) | 1267 |
| BisI | GCNGC | 4 cut(s) | 576, 761, 764, 945 |
| BlsI | GCNGC | 4 cut(s) | 577, 762, 765, 946 |
| Bme1390I | CCNGG | 1 cut(s) | 1188 |
| Bme18I | GGWCC | 4 cut(s) | 452, 1067, 1441, 1620 |
| BmgBI | CACGTC | 1 cut(s) | 241 |
| BmgT120I | GGNCC | 6 cut(s) | 452, 527, 1067, 1184, 1441, 1620 |
| BmiI | GGNNCC | 3 cut(s) | 310, 1185, 1443 |
| BmrFI | CCNGG | 1 cut(s) | 1188 |
| BmsI | GCATC | 5 cut(s) | 133, 681, 1414, 1532, 1586 |
| BpiI | GAAGAC | 1 cut(s) | 25 |
| BpmI | CTGGAG | 4 cut(s) | 705, 827, 1125, 1284 |
| Bpu10I | CCTNAGC | 1 cut(s) | 608 |
| BpuEI | CTTGAG | 3 cut(s) | 164, 472, 978 |
| BsaAI | YACGTR | 1 cut(s) | 875 |
| BsaBI | GATNNNNATC | 1 cut(s) | 375 |
| BsaI | GGTCTC | 2 cut(s) | 63, 94 |
| BsaJI | CCNNGG | 3 cut(s) | 84, 205, 289 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 852, 853, 1573 |
| Bse1I | ACTGG | 3 cut(s) | 271, 390, 1267 |
| Bse3DI | GCAATG | 2 cut(s) | 312, 642 |
| Bse8I | GATNNNNATC | 1 cut(s) | 375 |
| BseBI | CCWGG | 1 cut(s) | 1188 |
| BseDI | CCNNGG | 3 cut(s) | 84, 205, 289 |
| BseGI | GGATG | 2 cut(s) | 1150, 1410 |
| BseJI | GATNNNNATC | 1 cut(s) | 375 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 852, 853, 1573 |
| BseMI | GCAATG | 2 cut(s) | 312, 642 |
| BseMII | CTCAG | 4 cut(s) | 58, 622, 1130, 1606 |
| BseNI | ACTGG | 3 cut(s) | 271, 390, 1267 |
| BseRI | GAGGAG | 2 cut(s) | 939, 986 |
| BseXI | GCAGC | 4 cut(s) | 562, 750, 772, 956 |
| BseYI | CCCAGC | 2 cut(s) | 174, 1227 |
| BsgI | GTGCAG | 1 cut(s) | 963 |
| BshFI | GGCC | 8 cut(s) | 21, 529, 606, 743, 1186, 1223, 1293, 1371 |
| BshNI | GGYRCC | 1 cut(s) | 308 |
| BsiHKAI | GWGCWC | 3 cut(s) | 155, 880, 1605 |
| BslFI | GGGAC | 1 cut(s) | 1427 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 852, 853, 1573 |
| BsmAI | GTCTC | 3 cut(s) | 63, 94, 538 |
| BsmFI | GGGAC | 1 cut(s) | 1427 |
| BsnI | GGCC | 8 cut(s) | 21, 529, 606, 743, 1186, 1223, 1293, 1371 |
| Bso31I | GGTCTC | 2 cut(s) | 63, 94 |
| Bsp1286I | GDGCHC | 3 cut(s) | 155, 880, 1605 |
| Bsp143I | GATC | 3 cut(s) | 660, 670, 1267 |
| Bsp19I | CCATGG | 2 cut(s) | 84, 205 |
| BspANI | GGCC | 8 cut(s) | 21, 529, 606, 743, 1186, 1223, 1293, 1371 |
| BspCNI | CTCAG | 4 cut(s) | 57, 621, 1129, 1605 |
| BspHI | TCATGA | 1 cut(s) | 505 |
| BspLI | GGNNCC | 3 cut(s) | 310, 1185, 1443 |
| BspMI | ACCTGC | 1 cut(s) | 139 |
| BspT107I | GGYRCC | 1 cut(s) | 308 |
| BspTNI | GGTCTC | 2 cut(s) | 63, 94 |
| BsrDI | GCAATG | 2 cut(s) | 312, 642 |
| BsrI | ACTGG | 3 cut(s) | 271, 390, 1267 |
| BssECI | CCNNGG | 3 cut(s) | 84, 205, 289 |
| BssMI | GATC | 3 cut(s) | 660, 670, 1267 |
| BssT1I | CCWWGG | 3 cut(s) | 84, 205, 289 |
| Bst2UI | CCWGG | 1 cut(s) | 1188 |
| Bst4CI | ACNGT | 7 cut(s) | 343, 397, 826, 893, 911, 1475, 1620 |
| Bst6I | CTCTTC | 3 cut(s) | 104, 115, 595 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 308 |
| BstBAI | YACGTR | 1 cut(s) | 875 |
| BstC8I | GCNNGC | 3 cut(s) | 1090, 1373, 1466 |
| BstDEI | CTNAG | 5 cut(s) | 44, 608, 1116, 1569, 1592 |
| BstDSI | CCRYGG | 2 cut(s) | 84, 205 |
| BstEII | GGTNACC | 1 cut(s) | 841 |
| BstF5I | GGATG | 2 cut(s) | 1150, 1410 |
| BstHHI | GCGC | 2 cut(s) | 580, 1464 |
| BstKTI | GATC | 3 cut(s) | 663, 673, 1270 |
| BstMAI | GTCTC | 3 cut(s) | 63, 94, 538 |
| BstMBI | GATC | 3 cut(s) | 660, 670, 1267 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 308, 358 |
| BstNI | CCWGG | 1 cut(s) | 1188 |
| BstPI | GGTNACC | 1 cut(s) | 841 |
| BstSCI | CCNGG | 1 cut(s) | 1186 |
| BstV1I | GCAGC | 4 cut(s) | 562, 750, 772, 956 |
| BstV2I | GAAGAC | 1 cut(s) | 25 |
| BstX2I | RGATCY | 1 cut(s) | 1267 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 734 |
| BstYI | RGATCY | 1 cut(s) | 1267 |
| BsuI | GTATCC | 1 cut(s) | 1268 |
| BsuRI | GGCC | 8 cut(s) | 21, 529, 606, 743, 1186, 1223, 1293, 1371 |
| BtgI | CCRYGG | 2 cut(s) | 84, 205 |
| BtrI | CACGTC | 1 cut(s) | 241 |
| BtsCI | GGATG | 2 cut(s) | 1150, 1410 |
| BtsI | GCAGTG | 1 cut(s) | 447 |
| BtsIMutI | CAGTG | 5 cut(s) | 339, 447, 898, 1132, 1478 |
| BveI | ACCTGC | 1 cut(s) | 139 |
| Cac8I | GCNNGC | 3 cut(s) | 1090, 1373, 1466 |
| CciI | TCATGA | 1 cut(s) | 505 |
| CfoI | GCGC | 2 cut(s) | 580, 1464 |
| Cfr13I | GGNCC | 6 cut(s) | 452, 527, 1067, 1184, 1441, 1620 |
| CseI | GACGC | 1 cut(s) | 1409 |
| Csp6I | GTAC | 6 cut(s) | 586, 597, 835, 907, 1256, 1616 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 477, 512 |
| CviAII | CATG | 6 cut(s) | 85, 206, 236, 506, 632, 728 |
| CviQI | GTAC | 6 cut(s) | 586, 597, 835, 907, 1256, 1616 |
| DdeI | CTNAG | 5 cut(s) | 44, 608, 1116, 1569, 1592 |
| DpnI | GATC | 3 cut(s) | 662, 672, 1269 |
| DpnII | GATC | 3 cut(s) | 660, 670, 1267 |
| DraI | TTTAAA | 1 cut(s) | 429 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1406 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1406 |
| EaeI | YGGCCR | 3 cut(s) | 19, 741, 1291 |
| Eam1104I | CTCTTC | 3 cut(s) | 104, 115, 595 |
| EarI | CTCTTC | 3 cut(s) | 104, 115, 595 |
| Ecl136II | GAGCTC | 1 cut(s) | 1603 |
| Eco130I | CCWWGG | 3 cut(s) | 84, 205, 289 |
| Eco147I | AGGCCT | 2 cut(s) | 606, 1371 |
| Eco24I | GRGCYC | 1 cut(s) | 1605 |
| Eco31I | GGTCTC | 2 cut(s) | 63, 94 |
| Eco32I | GATATC | 1 cut(s) | 1495 |
| Eco47I | GGWCC | 4 cut(s) | 452, 1067, 1441, 1620 |
| Eco53kI | GAGCTC | 1 cut(s) | 1603 |
| Eco57I | CTGAAG | 2 cut(s) | 19, 1449 |
| Eco91I | GGTNACC | 1 cut(s) | 841 |
| EcoICRI | GAGCTC | 1 cut(s) | 1603 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1067, 1184, 1441 |
| EcoO65I | GGTNACC | 1 cut(s) | 841 |
| EcoRI | GAATTC | 1 cut(s) | 1054 |
| EcoRII | CCWGG | 1 cut(s) | 1186 |
| EcoRV | GATATC | 1 cut(s) | 1495 |
| EcoT14I | CCWWGG | 3 cut(s) | 84, 205, 289 |
| EcoT22I | ATGCAT | 1 cut(s) | 696 |
| EcoT38I | GRGCYC | 1 cut(s) | 1605 |
| ErhI | CCWWGG | 3 cut(s) | 84, 205, 289 |
| FaeI | CATG | 6 cut(s) | 88, 209, 239, 509, 635, 731 |
| FaqI | GGGAC | 1 cut(s) | 1427 |
| FatI | CATG | 6 cut(s) | 84, 205, 235, 505, 631, 727 |
| FauNDI | CATATG | 1 cut(s) | 1453 |
| Fnu4HI | GCNGC | 4 cut(s) | 576, 761, 764, 945 |
| FokI | GGATG | 2 cut(s) | 1137, 1397 |
| FriOI | GRGCYC | 1 cut(s) | 1605 |
| Fsp4HI | GCNGC | 4 cut(s) | 576, 761, 764, 945 |
| FspBI | CTAG | 4 cut(s) | 546, 953, 1061, 1376 |
| GlaI | GCGC | 2 cut(s) | 579, 1463 |
| GluI | GCNGC | 4 cut(s) | 576, 761, 764, 945 |
| GsaI | CCCAGC | 2 cut(s) | 178, 1231 |
| GsuI | CTGGAG | 4 cut(s) | 705, 827, 1125, 1284 |
| HaeIII | GGCC | 8 cut(s) | 21, 529, 606, 743, 1186, 1223, 1293, 1371 |
| HgaI | GACGC | 1 cut(s) | 1409 |
| HhaI | GCGC | 2 cut(s) | 580, 1464 |
| Hin1II | CATG | 6 cut(s) | 88, 209, 239, 509, 635, 731 |
| Hin6I | GCGC | 2 cut(s) | 578, 1462 |
| HinP1I | GCGC | 2 cut(s) | 578, 1462 |
| HincII | GTYRAC | 1 cut(s) | 1309 |
| HindII | GTYRAC | 1 cut(s) | 1309 |
| HinfI | GANTC | 8 cut(s) | 30, 371, 479, 622, 628, 798, 1112, 1513 |
| HphI | GGTGA | 5 cut(s) | 73, 430, 835, 1376, 1448 |
| Hpy166II | GTNNAC | 2 cut(s) | 1309, 1478 |
| Hpy188I | TCNGA | 9 cut(s) | 47, 157, 249, 349, 538, 627, 657, 1119, 1595 |
| Hpy8I | GTNNAC | 2 cut(s) | 1309, 1478 |
| Hpy99I | CGWCG | 1 cut(s) | 1545 |
| HpyAV | CCTTC | 3 cut(s) | 178, 415, 1361 |
| HpyCH4III | ACNGT | 7 cut(s) | 343, 397, 826, 893, 911, 1475, 1620 |
| HpyCH4IV | ACGT | 2 cut(s) | 240, 874 |
| HpyCH4V | TGCA | 6 cut(s) | 302, 317, 694, 944, 980, 1451 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 308, 358 |
| HpyF3I | CTNAG | 5 cut(s) | 44, 608, 1116, 1569, 1592 |
| HpySE526I | ACGT | 2 cut(s) | 240, 874 |
| Hsp92II | CATG | 6 cut(s) | 88, 209, 239, 509, 635, 731 |
| HspAI | GCGC | 2 cut(s) | 578, 1462 |
| Kzo9I | GATC | 3 cut(s) | 660, 670, 1267 |
| LmnI | GCTCC | 2 cut(s) | 262, 952 |
| Lsp1109I | GCAGC | 4 cut(s) | 562, 750, 772, 956 |
| LweI | GCATC | 5 cut(s) | 133, 681, 1414, 1532, 1586 |
| MaeI | CTAG | 4 cut(s) | 546, 953, 1061, 1376 |
| MaeII | ACGT | 2 cut(s) | 240, 874 |
| MaeIII | GTNAC | 3 cut(s) | 39, 841, 1546 |
| MalI | GATC | 3 cut(s) | 662, 672, 1269 |
| MboI | GATC | 3 cut(s) | 660, 670, 1267 |
| MfeI | CAATTG | 1 cut(s) | 1047 |
| MflI | RGATCY | 1 cut(s) | 1267 |
| MhlI | GDGCHC | 3 cut(s) | 155, 880, 1605 |
| MlsI | TGGCCA | 1 cut(s) | 1293 |
| MluNI | TGGCCA | 1 cut(s) | 1293 |
| MlyI | GAGTC | 3 cut(s) | 39, 622, 1522 |
| Mox20I | TGGCCA | 1 cut(s) | 1293 |
| Mph1103I | ATGCAT | 1 cut(s) | 696 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1042 |
| MscI | TGGCCA | 1 cut(s) | 1293 |
| MseI | TTAA | 8 cut(s) | 212, 228, 428, 558, 900, 1209, 1317, 1329 |
| Msp20I | TGGCCA | 1 cut(s) | 1293 |
| MspA1I | CMGCKG | 3 cut(s) | 763, 1088, 1358 |
| MspR9I | CCNGG | 1 cut(s) | 1188 |
| MunI | CAATTG | 1 cut(s) | 1047 |
| MvaI | CCWGG | 1 cut(s) | 1188 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 308, 358 |
| NcoI | CCATGG | 2 cut(s) | 84, 205 |
| NdeI | CATATG | 1 cut(s) | 1453 |
| NdeII | GATC | 3 cut(s) | 660, 670, 1267 |
| NlaIII | CATG | 6 cut(s) | 88, 209, 239, 509, 635, 731 |
| NlaIV | GGNNCC | 3 cut(s) | 310, 1185, 1443 |
| NmeAIII | GCCGAG | 1 cut(s) | 172 |
| NmuCI | GTSAC | 3 cut(s) | 39, 841, 1546 |
| NsiI | ATGCAT | 1 cut(s) | 696 |
| PagI | TCATGA | 1 cut(s) | 505 |
| PceI | AGGCCT | 2 cut(s) | 606, 1371 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1042 |
| PfeI | GAWTC | 5 cut(s) | 371, 479, 622, 798, 1112 |
| PkrI | GCNGC | 4 cut(s) | 577, 762, 765, 946 |
| PleI | GAGTC | 3 cut(s) | 38, 622, 1521 |
| PpsI | GAGTC | 3 cut(s) | 38, 622, 1521 |
| Ppu21I | YACGTR | 1 cut(s) | 875 |
| PpuMI | RGGWCCY | 2 cut(s) | 1067, 1441 |
| PsiI | TTATAA | 1 cut(s) | 392 |
| Psp124BI | GAGCTC | 1 cut(s) | 1605 |
| Psp5II | RGGWCCY | 2 cut(s) | 1067, 1441 |
| Psp6I | CCWGG | 1 cut(s) | 1186 |
| PspEI | GGTNACC | 1 cut(s) | 841 |
| PspFI | CCCAGC | 2 cut(s) | 174, 1227 |
| PspGI | CCWGG | 1 cut(s) | 1186 |
| PspN4I | GGNNCC | 3 cut(s) | 310, 1185, 1443 |
| PspPI | GGNCC | 6 cut(s) | 452, 527, 1067, 1184, 1441, 1620 |
| PspPPI | RGGWCCY | 2 cut(s) | 1067, 1441 |
| PsuI | RGATCY | 1 cut(s) | 1267 |
| PvuII | CAGCTG | 3 cut(s) | 763, 1088, 1358 |
| RsaI | GTAC | 6 cut(s) | 587, 598, 836, 908, 1257, 1617 |
| RsaNI | GTAC | 6 cut(s) | 586, 597, 835, 907, 1256, 1616 |
| SacI | GAGCTC | 1 cut(s) | 1605 |
| SaqAI | TTAA | 8 cut(s) | 212, 228, 428, 558, 900, 1209, 1317, 1329 |
| SatI | GCNGC | 4 cut(s) | 576, 761, 764, 945 |
| Sau3AI | GATC | 3 cut(s) | 660, 670, 1267 |
| Sau96I | GGNCC | 6 cut(s) | 452, 527, 1067, 1184, 1441, 1620 |
| SchI | GAGTC | 3 cut(s) | 39, 622, 1522 |
| ScrFI | CCNGG | 1 cut(s) | 1188 |
| SduI | GDGCHC | 3 cut(s) | 155, 880, 1605 |
| SfaNI | GCATC | 5 cut(s) | 133, 681, 1414, 1532, 1586 |
| SinI | GGWCC | 4 cut(s) | 452, 1067, 1441, 1620 |
| SmlI | CTYRAG | 3 cut(s) | 179, 487, 993 |
| SmoI | CTYRAG | 3 cut(s) | 179, 487, 993 |
| SseBI | AGGCCT | 2 cut(s) | 606, 1371 |
| SspI | AATATT | 2 cut(s) | 931, 1395 |
| SspMI | CTAG | 4 cut(s) | 546, 953, 1061, 1376 |
| SstI | GAGCTC | 1 cut(s) | 1605 |
| StuI | AGGCCT | 2 cut(s) | 606, 1371 |
| StyD4I | CCNGG | 1 cut(s) | 1186 |
| StyI | CCWWGG | 3 cut(s) | 84, 205, 289 |
| TaaI | ACNGT | 7 cut(s) | 343, 397, 826, 893, 911, 1475, 1620 |
| TaiI | ACGT | 2 cut(s) | 243, 877 |
| TaqI | TCGA | 3 cut(s) | 788, 1072, 1608 |
| TaqII | GACCGA | 3 cut(s) | 469, 1326, 1499 |
| TatI | WGTACW | 3 cut(s) | 585, 834, 906 |
| TfiI | GAWTC | 5 cut(s) | 371, 479, 622, 798, 1112 |
| Tru1I | TTAA | 8 cut(s) | 212, 228, 428, 558, 900, 1209, 1317, 1329 |
| Tru9I | TTAA | 8 cut(s) | 212, 228, 428, 558, 900, 1209, 1317, 1329 |
| TscAI | CASTG | 5 cut(s) | 346, 447, 898, 1132, 1485 |
| TseFI | GTSAC | 3 cut(s) | 39, 841, 1546 |
| TseI | GCWGC | 4 cut(s) | 575, 760, 763, 944 |
| Tsp45I | GTSAC | 3 cut(s) | 39, 841, 1546 |
| TspDTI | ATGAA | 6 cut(s) | 453, 494, 631, 906, 1299, 1543 |
| TspRI | CASTG | 5 cut(s) | 346, 447, 898, 1132, 1485 |
| VpaK11BI | GGWCC | 4 cut(s) | 452, 1067, 1441, 1620 |
| XapI | RAATTY | 2 cut(s) | 637, 1054 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 382, 802 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1042 |
| XspI | CTAG | 4 cut(s) | 546, 953, 1061, 1376 |
| Zsp2I | ATGCAT | 1 cut(s) | 696 |