MD02G1119800.v1.1

Adipocyte plasma membrane-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000797
Physical Location & Seq
Reverse (-)
9724829 .. 9730057
5229 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1119800.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGCACTCCCCTTCCCCAGCCCAAGCCTCCGACGGCGCTCATCCCACTCCCACAAGAAAAACTCCTTCATGGCACTCAACTCTTCTCATCTTCTCAGTACTAGCTCCAGTAGCAATAGCCCTACTCATTTACCAACTCGACACGCTCAACCCAGCTCCTCTGCCGCTTCACGAGTTGACTCAACCAGTCGCAGCTGCTCCGAAGCGTAATGCTCATATGCTCAAAGGGTCGGAGTTTGTGGGTGCCGGGGCTTTAATGGCACCGGAAGACGTGGCTTACGATCCGAAGTCGGGATTTATTTATACCGGATGTGCTGATGGGTGGGTTAAACGAGTCACGCTGAACGAGTCGGCTGCTGACTCGGTGGTGGAGAACTGGGTTTTCACCGGCGGGAGGCCACTCGGACTTGCTCACGGCGCTGACAAGGAGGTTTACGTGGCTGACACGGAAAAGGGGCTACTGAAGATAAGTGAGGAGGGAACAATCGAACTGCTGACAGATGAGGCCGAGGGTGTAAAATTCAAGCTAACAGATTTTGTAGATGTTGCAGAAGATGGTATGATATATTTCACAGATGCTTCATACAAATACAGCTTGCATGAGTTCATGTGGGACATTTTAGAAGGTAGACCCTACGGTAGATTTCTGAGCTACAATCCGACGACCAAAGAGACCAAAGTGCTCGTTCGCAACCTGTACTTTGCTAATGGACTTGTGGTCTCTCCCGATCAAAACTATGTCATCTTCTGCGAAACTGTCATGAGAAGGTGTAGGAAATATTACCTGCAAGGCACTAAAAAAGGGAGTGTAGAAAACTTTATCGACCAGTTACCGGGCATGCCTGATAATATCAGATATGATGGAGAAGGCCGCTACTGGATTGCACTGGCCACGGATGTTAAACCATACTGGGATCTAGCACTTCGATATCCTTTTATTCGGAAAGTCCTAGCGATCGTGAAGCGGTATGCAGGAGGCCGGCCATATTTGGAGAAGAATGCTGGCGTATTTGCTGTGAATTTGGATGGGGAGCCGATTGCTCATTACTCTGATCCTGAGTTGTTTCTTATTACAAGTGGGATCAAGATTGGAAAGTTCCTCTACTGCGGTTCCGTCGTCTATCCCTACATTATCCGCCTCGATCTGGATCGACATCCTGCACATGCCACCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

392

Amino Acids

43.57

Weight (kDa)

5.96

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSL_N PF20067 74 - 112 9.4e-06 Strictosidine synthase-like, N-terminal
SGL PF08450 126 - 297 1.5e-11 SMP-30/Gluconolactonase/LRE-like region
Str_synth PF03088 179 - 264 1.2e-32 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 792
AccB1I GGYRCC 2 cut(s) 242, 259
AccI GTMKAC 1 cut(s) 628
AciI CCGC 6 cut(s) 164, 390, 871, 964, 1107, 1135
AclWI GGATC 5 cut(s) 275, 921, 1046, 1088, 1155
AcoI YGGCCR 2 cut(s) 888, 980
AcsI RAATTY 2 cut(s) 518, 1018
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 2 cut(s) 99, 698
AfiI CCNNNNNNNGG 4 cut(s) 290, 393, 832, 1144
AgsI TTSAA 1 cut(s) 523
AhdI GACNNNNNGTC 1 cut(s) 716
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 6 cut(s) 104, 155, 194, 526, 594, 651
AluI AGCT 6 cut(s) 104, 155, 194, 526, 594, 651
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 2 cut(s) 665, 724
AlwI GGATC 5 cut(s) 275, 921, 1046, 1088, 1155
AoxI GGCC 6 cut(s) 395, 504, 868, 888, 976, 980
ApeKI GCWGC 3 cut(s) 191, 194, 353
ApoI RAATTY 2 cut(s) 518, 1018
AspLEI GCGC 2 cut(s) 38, 419
AsuC2I CCSGG 2 cut(s) 247, 834
AsuHPI GGTGA 1 cut(s) 376
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 2 cut(s) 242, 259
BauI CACGAG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 181, 203, 340
BccI CCATC 4 cut(s) 311, 548, 854, 1019
BceAI ACGGC 2 cut(s) 49, 430
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BcnI CCSGG 2 cut(s) 247, 834
BcoDI GTCTC 2 cut(s) 665, 724
BfaI CTAG 3 cut(s) 101, 917, 950
BfoI RGCGCY 2 cut(s) 39, 420
BfuAI ACCTGC 1 cut(s) 792
BisI GCNGC 5 cut(s) 164, 192, 195, 354, 871
BlsI GCNGC 5 cut(s) 165, 193, 196, 355, 872
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 247, 834
BmeRI GACNNNNNGTC 1 cut(s) 716
BmgBI CACGTC 1 cut(s) 271
BmiI GGNNCC 4 cut(s) 244, 261, 1032, 1111
BmrFI CCNGG 2 cut(s) 247, 834
BmrI ACTGGG 2 cut(s) 385, 919
BmsI GCATC 1 cut(s) 565
BmuI ACTGGG 2 cut(s) 385, 919
BpiI GAAGAC 1 cut(s) 273
BpmI CTGGAG 1 cut(s) 90
BpuMI CCSGG 2 cut(s) 247, 834
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 2 cut(s) 1146, 1152
BsaI GGTCTC 2 cut(s) 665, 724
BsaJI CCNNGG 3 cut(s) 246, 507, 891
BsaWI WCCGGW 2 cut(s) 262, 305
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 393, 832, 1144
Bse118I RCCGGY 2 cut(s) 386, 978
Bse1I ACTGG 7 cut(s) 107, 185, 380, 826, 881, 891, 914
Bse8I GATNNNNATC 2 cut(s) 1146, 1152
BseDI CCNNGG 3 cut(s) 246, 507, 891
BseGI GGATG 5 cut(s) 40, 314, 901, 1030, 1153
BseJI GATNNNNATC 2 cut(s) 1146, 1152
BseLI CCNNNNNNNGG 4 cut(s) 290, 393, 832, 1144
BseMII CTCAG 3 cut(s) 108, 638, 1047
BseNI ACTGG 7 cut(s) 107, 185, 380, 826, 881, 891, 914
BseRI GAGGAG 2 cut(s) 147, 488
BseXI GCAGC 3 cut(s) 181, 203, 340
BseYI CCCAGC 2 cut(s) 16, 151
BsgI GTGCAG 1 cut(s) 1143
Bsh1285I CGRYCG 1 cut(s) 957
BshFI GGCC 6 cut(s) 397, 506, 870, 890, 978, 982
BshNI GGYRCC 2 cut(s) 242, 259
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 684
BsiSI CCGG 6 cut(s) 246, 263, 306, 387, 833, 979
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 4 cut(s) 290, 393, 832, 1144
BsmAI GTCTC 2 cut(s) 665, 724
BsmFI GGGAC 1 cut(s) 626
BsmI GAATGC 1 cut(s) 1003
BsnI GGCC 6 cut(s) 397, 506, 870, 890, 978, 982
Bso31I GGTCTC 2 cut(s) 665, 724
Bsp1286I GDGCHC 1 cut(s) 684
Bsp143I GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
BspACI CCGC 6 cut(s) 164, 390, 871, 964, 1107, 1135
BspANI GGCC 6 cut(s) 397, 506, 870, 890, 978, 982
BspCNI CTCAG 3 cut(s) 107, 639, 1048
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 4 cut(s) 244, 261, 1032, 1111
BspMI ACCTGC 1 cut(s) 792
BspPI GGATC 5 cut(s) 275, 921, 1046, 1088, 1155
BspT107I GGYRCC 2 cut(s) 242, 259
BspTNI GGTCTC 2 cut(s) 665, 724
BsrFI RCCGGY 2 cut(s) 386, 978
BsrI ACTGG 7 cut(s) 107, 185, 380, 826, 881, 891, 914
BssAI RCCGGY 2 cut(s) 386, 978
BssECI CCNNGG 3 cut(s) 246, 507, 891
BssMI GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
BssSI CACGAG 1 cut(s) 170
Bst2BI CACGAG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 638, 757
Bst6I CTCTTC 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 436
BstC8I GCNNGC 4 cut(s) 596, 839, 980, 1003
BstDEI CTNAG 3 cut(s) 94, 647, 1056
BstDSI CCRYGG 1 cut(s) 891
BstF5I GGATG 5 cut(s) 40, 314, 901, 1030, 1153
BstH2I RGCGCY 2 cut(s) 39, 420
BstHHI GCGC 2 cut(s) 38, 419
BstKTI GATC 8 cut(s) 283, 730, 916, 957, 1054, 1083, 1144, 1150
BstMAI GTCTC 2 cut(s) 665, 724
BstMBI GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 3 cut(s) 110, 257, 416
BstNSI RCATGY 2 cut(s) 841, 1166
BstSCI CCNGG 2 cut(s) 245, 832
BstV1I GCAGC 3 cut(s) 181, 203, 340
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
BsuRI GGCC 6 cut(s) 397, 506, 870, 890, 978, 982
BtgI CCRYGG 1 cut(s) 891
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 5 cut(s) 40, 314, 901, 1030, 1153
BtsIMutI CAGTG 1 cut(s) 884
BveI ACCTGC 1 cut(s) 792
Cac8I GCNNGC 4 cut(s) 596, 839, 980, 1003
CciI TCATGA 1 cut(s) 759
CfoI GCGC 2 cut(s) 38, 419
Cfr10I RCCGGY 2 cut(s) 386, 978
Csp6I GTAC 2 cut(s) 98, 697
CviAII CATG 7 cut(s) 69, 599, 607, 760, 838, 1163, 1173
CviQI GTAC 2 cut(s) 98, 697
DdeI CTNAG 3 cut(s) 94, 647, 1056
DpnI GATC 8 cut(s) 282, 729, 915, 956, 1053, 1082, 1143, 1149
DpnII GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
DriI GACNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 888, 980
Eam1104I CTCTTC 1 cut(s) 87
Eam1105I GACNNNNNGTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 1124
Eco31I GGTCTC 2 cut(s) 665, 724
Eco32I GATATC 1 cut(s) 929
Eco57I CTGAAG 1 cut(s) 482
EcoRV GATATC 1 cut(s) 929
FaeI CATG 7 cut(s) 72, 602, 610, 763, 841, 1166, 1176
FaqI GGGAC 1 cut(s) 626
FatI CATG 7 cut(s) 68, 598, 606, 759, 837, 1162, 1172
FauI CCCGC 1 cut(s) 383
FauNDI CATATG 1 cut(s) 216
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 5 cut(s) 164, 192, 195, 354, 871
FokI GGATG 5 cut(s) 27, 321, 908, 1037, 1140
FseI GGCCGGCC 1 cut(s) 982
Fsp4HI GCNGC 5 cut(s) 164, 192, 195, 354, 871
FspBI CTAG 3 cut(s) 101, 917, 950
GlaI GCGC 2 cut(s) 37, 418
GluI GCNGC 5 cut(s) 164, 192, 195, 354, 871
GsaI CCCAGC 2 cut(s) 20, 155
GsuI CTGGAG 1 cut(s) 90
HaeII RGCGCY 2 cut(s) 39, 420
HaeIII GGCC 6 cut(s) 397, 506, 870, 890, 978, 982
HapII CCGG 6 cut(s) 246, 263, 306, 387, 833, 979
HhaI GCGC 2 cut(s) 38, 419
Hin1II CATG 7 cut(s) 72, 602, 610, 763, 841, 1166, 1176
Hin6I GCGC 2 cut(s) 36, 417
HinP1I GCGC 2 cut(s) 36, 417
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HinfI GANTC 4 cut(s) 178, 333, 347, 359
HpaII CCGG 6 cut(s) 246, 263, 306, 387, 833, 979
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 3 cut(s) 177, 433, 629
Hpy188III TCNNGA 8 cut(s) 170, 291, 725, 760, 958, 1055, 1084, 1145
Hpy8I GTNNAC 3 cut(s) 177, 433, 629
Hpy99I CGWCG 3 cut(s) 35, 664, 1118
HpyAV CCTTC 5 cut(s) 21, 75, 617, 759, 860
HpyCH4III ACNGT 2 cut(s) 638, 757
HpyCH4IV ACGT 2 cut(s) 270, 435
HpyCH4V TGCA 7 cut(s) 4, 548, 598, 787, 884, 971, 1160
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 257, 416
HpyF3I CTNAG 3 cut(s) 94, 647, 1056
HpySE526I ACGT 2 cut(s) 270, 435
Hsp92II CATG 7 cut(s) 72, 602, 610, 763, 841, 1166, 1176
HspAI GCGC 2 cut(s) 36, 417
KroI GCCGGC 1 cut(s) 978
KroNI GCCGGC 1 cut(s) 980
Kzo9I GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
LmnI GCTCC 4 cut(s) 109, 160, 202, 1030
Lsp1109I GCAGC 3 cut(s) 181, 203, 340
LweI GCATC 1 cut(s) 565
MaeI CTAG 3 cut(s) 101, 917, 950
MaeII ACGT 2 cut(s) 270, 435
MaeIII GTNAC 2 cut(s) 334, 828
MalI GATC 8 cut(s) 282, 729, 915, 956, 1053, 1082, 1143, 1149
MboI GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
MboII GAAGA 7 cut(s) 74, 82, 278, 475, 563, 736, 1006
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 1 cut(s) 684
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 2 cut(s) 518, 1018
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 4 cut(s) 172, 342, 353, 356
MmeI TCCRAC 3 cut(s) 54, 210, 683
Mox20I TGGCCA 1 cut(s) 890
MroNI GCCGGC 1 cut(s) 978
MscI TGGCCA 1 cut(s) 890
MseI TTAA 3 cut(s) 254, 327, 900
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 6 cut(s) 246, 263, 306, 387, 833, 979
MspR9I CCNGG 2 cut(s) 247, 834
Mva1269I GAATGC 1 cut(s) 1003
MwoI GCNNNNNNNGC 3 cut(s) 110, 257, 416
NaeI GCCGGC 1 cut(s) 980
NciI CCSGG 2 cut(s) 247, 834
NdeI CATATG 1 cut(s) 216
NdeII GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
NgoMIV GCCGGC 1 cut(s) 978
NlaIII CATG 7 cut(s) 72, 602, 610, 763, 841, 1166, 1176
NlaIV GGNNCC 4 cut(s) 244, 261, 1032, 1111
NmeAIII GCCGAG 1 cut(s) 532
NmuCI GTSAC 1 cut(s) 334
NspI RCATGY 2 cut(s) 841, 1166
PaeI GCATGC 1 cut(s) 841
PagI TCATGA 1 cut(s) 759
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1003
PdiI GCCGGC 1 cut(s) 980
PkrI GCNGC 5 cut(s) 165, 193, 196, 355, 872
Ple19I CGATCG 1 cut(s) 957
PleI GAGTC 4 cut(s) 172, 341, 353, 355
PpsI GAGTC 4 cut(s) 172, 341, 353, 355
Ppu21I YACGTR 1 cut(s) 436
PspFI CCCAGC 2 cut(s) 16, 151
PspN4I GGNNCC 4 cut(s) 244, 261, 1032, 1111
PsuI RGATCY 1 cut(s) 913
PvuI CGATCG 1 cut(s) 957
PvuII CAGCTG 1 cut(s) 194
RigI GGCCGGCC 1 cut(s) 982
RsaI GTAC 2 cut(s) 99, 698
RsaNI GTAC 2 cut(s) 98, 697
SaqAI TTAA 3 cut(s) 254, 327, 900
SatI GCNGC 5 cut(s) 164, 192, 195, 354, 871
Sau3AI GATC 8 cut(s) 280, 727, 913, 954, 1051, 1080, 1141, 1147
ScaI AGTACT 1 cut(s) 99
SchI GAGTC 4 cut(s) 172, 342, 353, 356
ScrFI CCNGG 2 cut(s) 247, 834
SduI GDGCHC 1 cut(s) 684
SfaNI GCATC 1 cut(s) 565
SgrAI CRCCGGYG 1 cut(s) 386
SphI GCATGC 1 cut(s) 841
Sse9I AATT 2 cut(s) 518, 1018
SsiI CCGC 6 cut(s) 164, 390, 871, 964, 1107, 1135
SspI AATATT 1 cut(s) 779
SspMI CTAG 3 cut(s) 101, 917, 950
StyD4I CCNGG 2 cut(s) 245, 832
TaaI ACNGT 2 cut(s) 638, 757
TaiI ACGT 2 cut(s) 273, 438
TaqI TCGA 6 cut(s) 138, 486, 822, 925, 1140, 1150
TasI AATT 2 cut(s) 518, 1018
TatI WGTACW 2 cut(s) 97, 696
TauI GCSGC 2 cut(s) 166, 873
Tru1I TTAA 3 cut(s) 254, 327, 900
Tru9I TTAA 3 cut(s) 254, 327, 900
TscAI CASTG 1 cut(s) 891
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 191, 194, 353
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 3 cut(s) 57, 570, 595
TspGWI ACGGA 3 cut(s) 461, 908, 1102
TspRI CASTG 1 cut(s) 891
XapI RAATTY 2 cut(s) 518, 1018
XceI RCATGY 2 cut(s) 841, 1166
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 3 cut(s) 101, 917, 950
ZrmI AGTACT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.