pycom02g09350

Adipocyte plasma membrane-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000797
Physical Location & Seq
Reverse (-)
6418527 .. 6422275
3749 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09350.3

Sequence Viewer

Length: 1170 bp
ATGAACTCAGCAGACTCGGTTTCTGTACCCAAACCCAGCTCCAAATCATCATGGCTCTCAGCTCCAACTTCTCTGTTTCTAATCTTGATCATCCCTATTTTTGCTGCAACCCTAATCTACCAACTCGACTCGTTCGACCTGGCCCCCATGCCGTTTCGTGAGTTGACTCGGCACTTAATCAAGAAGGTCCCAGCTCAAAACCCTCGCATGCTTCGAGGAGCTGAGCGTGTGGGGTATGGAGTCCTGCTGGGGCCGGAGGACGTGGCTTATGATAAGAGATCGGGGATTATTTACACTGGCTGTGCAGACGGGTGGATGAAGCGAATCAAGAGGAACGAGTCGGAGAATCCCGAGTTTGTGGTGGAGAATTGGGTCAACACGGGCGGCAGACCACTCGGACTCACTTTCGGGCATCACGATGAAGTTCTAGTGGCCGATGCCGACAAGGGCTTGCTGAATGTAACAAGTGAGGGTGAGGTAAAGGTGCTGGCAGACAAGGCTGAGGGTGTAAAATTTTTACTGACGGAGACTGTAGATGTAGCAGTGGATGGCATGATTTATTTCACAGATGCTTCATATAAATACAACATGAAAGACCATGTTTGGGACATTTTGGAGGGCCGGCCTCACGGAAGACTCATGAGCTACGATTCAGCAACCAAACAGACCACAGTGCTGCTGCGCGATCTGTACTTCGCTAATGGAGTGGTGGTTTCACCGGATCAGAGTCACGTAATCTACTGCGAAACCCCAATGAGGAGGTGTAGAAAATTTTACATACAAGGTGAGAAAAGGGGAAGCGTGGATGCTTTTATTGATCATCTTCCAGGCTTGCCAGATAACATAAGATATGATGGAGAAGGCCATTACTGGATTGCAATGTCAATGGGGCTTTCATTGGCATGGGATTTGGCTGTTAGATATCCTTTCATAAGAAAAGTGATGGCAATTATGGAGAAATACAAATTAAGACCCAGTGTGGAGAAGAATGGTGGAGTTCTGGCCGTTGATATGGAAGGAAATTTGATTGCTCACTACTATGATCCAGGATTGTCAATAATTTCAAGTGGCATCAAGATTGGTAATTACATATACTGCGGTTCGCCGATTTATCCTTACATGCTCCGCCTTAACATTCAAGAAAACCCACCTATTGCAGCCATTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

390

Amino Acids

43.58

Weight (kDa)

6.33

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 684
AciI CCGC 3 cut(s) 384, 1098, 1126
AclWI GGATC 2 cut(s) 729, 1037
AcoI YGGCCR 2 cut(s) 432, 1002
AcsI RAATTY 3 cut(s) 512, 770, 1021
AfaI GTAC 2 cut(s) 27, 692
AfiI CCNNNNNNNGG 2 cut(s) 604, 756
AgsI TTSAA 2 cut(s) 1065, 1139
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 3 cut(s) 138, 826, 1045
AluBI AGCT 5 cut(s) 39, 62, 194, 221, 645
AluI AGCT 5 cut(s) 39, 62, 194, 221, 645
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 2 cut(s) 729, 1037
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 7 cut(s) 141, 251, 432, 619, 623, 862, 1002
ApeKI GCWGC 4 cut(s) 104, 676, 679, 1157
ApoI RAATTY 3 cut(s) 512, 770, 1021
AspLEI GCGC 1 cut(s) 684
AspS9I GGNCC 4 cut(s) 142, 187, 251, 619
AsuHPI GGTGA 3 cut(s) 485, 708, 797
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 1 cut(s) 187
BbsI GAAGAC 1 cut(s) 640
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 3 cut(s) 91, 663, 666
BccI CCATC 3 cut(s) 542, 848, 937
BceAI ACGGC 2 cut(s) 136, 989
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BciT130I CCWGG 3 cut(s) 140, 828, 1047
BclI TGATCA 2 cut(s) 87, 817
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 531
BisI GCNGC 5 cut(s) 105, 385, 677, 680, 1158
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 5 cut(s) 106, 386, 678, 681, 1159
Bme1390I CCNGG 3 cut(s) 140, 828, 1047
Bme18I GGWCC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 142, 187, 251, 619
BmiI GGNNCC 3 cut(s) 144, 189, 252
BmrFI CCNGG 3 cut(s) 140, 828, 1047
BmrI ACTGGG 1 cut(s) 969
BmsI GCATC 5 cut(s) 421, 427, 559, 796, 1080
BmuI ACTGGG 1 cut(s) 969
BpiI GAAGAC 1 cut(s) 640
Bpu10I CCTNAGC 1 cut(s) 501
Bpu1102I GCTNAGC 1 cut(s) 222
BsaAI YACGTR 1 cut(s) 733
BsaWI WCCGGW 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 756
Bse118I RCCGGY 1 cut(s) 621
Bse1I ACTGG 3 cut(s) 301, 875, 975
Bse3DI GCAATG 1 cut(s) 885
BseBI CCWGG 3 cut(s) 140, 828, 1047
BseGI GGATG 4 cut(s) 90, 321, 553, 811
BseLI CCNNNNNNNGG 2 cut(s) 604, 756
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 4 cut(s) 21, 72, 213, 492
BseNI ACTGG 3 cut(s) 301, 875, 975
BseRI GAGGAG 2 cut(s) 231, 772
BseXI GCAGC 3 cut(s) 91, 663, 666
BseYI CCCAGC 3 cut(s) 35, 190, 247
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 684
BshFI GGCC 7 cut(s) 143, 253, 434, 621, 625, 864, 1004
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 3 cut(s) 254, 622, 719
BslFI GGGAC 2 cut(s) 173, 620
BslI CCNNNNNNNGG 2 cut(s) 604, 756
BsmAI GTCTC 1 cut(s) 521
BsmFI GGGAC 2 cut(s) 173, 620
BsnI GGCC 7 cut(s) 143, 253, 434, 621, 625, 864, 1004
BsoBI CYCGRG 1 cut(s) 350
Bsp143I GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
Bsp1720I GCTNAGC 1 cut(s) 222
BspACI CCGC 3 cut(s) 384, 1098, 1126
BspANI GGCC 7 cut(s) 143, 253, 434, 621, 625, 864, 1004
BspCNI CTCAG 4 cut(s) 20, 71, 214, 493
BspFNI CGCG 1 cut(s) 684
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 3 cut(s) 144, 189, 252
BspPI GGATC 2 cut(s) 729, 1037
BsrDI GCAATG 1 cut(s) 885
BsrFI RCCGGY 1 cut(s) 621
BsrI ACTGG 3 cut(s) 301, 875, 975
BssAI RCCGGY 1 cut(s) 621
BssMI GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
Bst2UI CCWGG 3 cut(s) 140, 828, 1047
Bst4CI ACNGT 2 cut(s) 532, 673
BstBAI YACGTR 1 cut(s) 733
BstC8I GCNNGC 5 cut(s) 209, 452, 489, 623, 833
BstDEI CTNAG 4 cut(s) 7, 58, 222, 501
BstF5I GGATG 4 cut(s) 90, 321, 553, 811
BstFNI CGCG 1 cut(s) 684
BstHHI GCGC 1 cut(s) 684
BstKTI GATC 6 cut(s) 90, 281, 688, 724, 820, 1045
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 3 cut(s) 140, 828, 1047
BstNSI RCATGY 2 cut(s) 211, 1123
BstSCI CCNGG 3 cut(s) 138, 826, 1045
BstSFI CTRYAG 1 cut(s) 531
BstUI CGCG 1 cut(s) 684
BstV1I GCAGC 3 cut(s) 91, 663, 666
BstV2I GAAGAC 1 cut(s) 640
BsuRI GGCC 7 cut(s) 143, 253, 434, 621, 625, 864, 1004
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 4 cut(s) 90, 321, 553, 811
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 4 cut(s) 294, 549, 678, 982
Cac8I GCNNGC 5 cut(s) 209, 452, 489, 623, 833
CciI TCATGA 1 cut(s) 639
CfoI GCGC 1 cut(s) 684
Cfr10I RCCGGY 1 cut(s) 621
Cfr13I GGNCC 4 cut(s) 142, 187, 251, 619
Csp6I GTAC 2 cut(s) 26, 691
CviAII CATG 9 cut(s) 51, 148, 208, 553, 589, 599, 640, 903, 1120
CviQI GTAC 2 cut(s) 26, 691
DdeI CTNAG 4 cut(s) 7, 58, 222, 501
DpnI GATC 6 cut(s) 89, 280, 687, 723, 819, 1044
DpnII GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
EaeI YGGCCR 2 cut(s) 432, 1002
EciI GGCGGA 1 cut(s) 1115
Eco32I GATATC 1 cut(s) 923
Eco47I GGWCC 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 187
EcoRII CCWGG 3 cut(s) 138, 826, 1045
EcoRV GATATC 1 cut(s) 923
FaeI CATG 9 cut(s) 54, 151, 211, 556, 592, 602, 643, 906, 1123
FaqI GGGAC 2 cut(s) 173, 620
FatI CATG 9 cut(s) 50, 147, 207, 552, 588, 598, 639, 902, 1119
FbaI TGATCA 2 cut(s) 87, 817
Fnu4HI GCNGC 5 cut(s) 105, 385, 677, 680, 1158
FokI GGATG 4 cut(s) 77, 328, 560, 818
FseI GGCCGGCC 1 cut(s) 625
Fsp4HI GCNGC 5 cut(s) 105, 385, 677, 680, 1158
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 683
GluI GCNGC 5 cut(s) 105, 385, 677, 680, 1158
GsaI CCCAGC 3 cut(s) 39, 194, 251
HaeIII GGCC 7 cut(s) 143, 253, 434, 621, 625, 864, 1004
HapII CCGG 3 cut(s) 254, 622, 719
HhaI GCGC 1 cut(s) 684
Hin1II CATG 9 cut(s) 54, 151, 211, 556, 592, 602, 643, 906, 1123
Hin6I GCGC 1 cut(s) 682
HinP1I GCGC 1 cut(s) 682
HincII GTYRAC 2 cut(s) 165, 376
HindII GTYRAC 2 cut(s) 165, 376
HpaII CCGG 3 cut(s) 254, 622, 719
HphI GGTGA 3 cut(s) 485, 708, 797
Hpy166II GTNNAC 2 cut(s) 165, 376
Hpy188I TCNGA 3 cut(s) 343, 398, 726
Hpy188III TCNNGA 9 cut(s) 85, 158, 181, 328, 350, 416, 640, 1075, 1139
Hpy8I GTNNAC 2 cut(s) 165, 376
HpyAV CCTTC 3 cut(s) 178, 854, 1010
HpyCH4III ACNGT 2 cut(s) 532, 673
HpyCH4IV ACGT 2 cut(s) 261, 732
HpyCH4V TGCA 4 cut(s) 107, 305, 878, 1157
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 4 cut(s) 7, 58, 222, 501
HpySE526I ACGT 2 cut(s) 261, 732
Hsp92II CATG 9 cut(s) 54, 151, 211, 556, 592, 602, 643, 906, 1123
HspAI GCGC 1 cut(s) 682
KroI GCCGGC 1 cut(s) 621
KroNI GCCGGC 1 cut(s) 623
Ksp22I TGATCA 2 cut(s) 87, 817
Kzo9I GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
LmnI GCTCC 4 cut(s) 44, 67, 218, 1128
Lsp1109I GCAGC 3 cut(s) 91, 663, 666
LweI GCATC 5 cut(s) 421, 427, 559, 796, 1080
MaeI CTAG 1 cut(s) 428
MaeII ACGT 2 cut(s) 261, 732
MaeIII GTNAC 2 cut(s) 460, 728
MalI GATC 6 cut(s) 89, 280, 687, 723, 819, 1044
MboI GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
MboII GAAGA 3 cut(s) 645, 815, 997
MluCI AATT 8 cut(s) 367, 512, 770, 948, 965, 1021, 1059, 1084
MlyI GAGTC 8 cut(s) 8, 122, 160, 249, 347, 393, 630, 736
MmeI TCCRAC 2 cut(s) 89, 321
MroNI GCCGGC 1 cut(s) 621
MseI TTAA 3 cut(s) 176, 968, 1131
MslI CAYNNNNRTG 3 cut(s) 417, 901, 1038
MspI CCGG 3 cut(s) 254, 622, 719
MspR9I CCNGG 3 cut(s) 140, 828, 1047
MvaI CCWGG 3 cut(s) 140, 828, 1047
MvnI CGCG 1 cut(s) 684
MwoI GCNNNNNNNGC 1 cut(s) 497
NaeI GCCGGC 1 cut(s) 623
NdeII GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
NgoMIV GCCGGC 1 cut(s) 621
NlaIII CATG 9 cut(s) 54, 151, 211, 556, 592, 602, 643, 906, 1123
NlaIV GGNNCC 3 cut(s) 144, 189, 252
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 728
NspI RCATGY 2 cut(s) 211, 1123
PaeI GCATGC 1 cut(s) 211
PagI TCATGA 1 cut(s) 639
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 211, 414
PdiI GCCGGC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 324, 346, 650
PfoI TCCNGGA 1 cut(s) 1045
PkrI GCNGC 5 cut(s) 106, 386, 678, 681, 1159
PleI GAGTC 8 cut(s) 8, 122, 160, 248, 346, 393, 630, 735
PpsI GAGTC 8 cut(s) 8, 122, 160, 248, 346, 393, 630, 735
Ppu21I YACGTR 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 187
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 3 cut(s) 138, 826, 1045
PspFI CCCAGC 3 cut(s) 35, 190, 247
PspGI CCWGG 3 cut(s) 138, 826, 1045
PspN4I GGNNCC 3 cut(s) 144, 189, 252
PspPI GGNCC 4 cut(s) 142, 187, 251, 619
PspPPI RGGWCCY 1 cut(s) 187
RigI GGCCGGCC 1 cut(s) 625
RsaI GTAC 2 cut(s) 27, 692
RsaNI GTAC 2 cut(s) 26, 691
RseI CAYNNNNRTG 3 cut(s) 417, 901, 1038
SaqAI TTAA 3 cut(s) 176, 968, 1131
SatI GCNGC 5 cut(s) 105, 385, 677, 680, 1158
Sau3AI GATC 6 cut(s) 87, 278, 685, 721, 817, 1042
Sau96I GGNCC 4 cut(s) 142, 187, 251, 619
SchI GAGTC 8 cut(s) 8, 122, 160, 249, 347, 393, 630, 736
ScrFI CCNGG 3 cut(s) 140, 828, 1047
SfaNI GCATC 5 cut(s) 421, 427, 559, 796, 1080
SfcI CTRYAG 1 cut(s) 531
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 3 cut(s) 417, 901, 1038
SphI GCATGC 1 cut(s) 211
Sse9I AATT 8 cut(s) 367, 512, 770, 948, 965, 1021, 1059, 1084
SsiI CCGC 3 cut(s) 384, 1098, 1126
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 138, 826, 1045
TaaI ACNGT 2 cut(s) 532, 673
TaiI ACGT 2 cut(s) 264, 735
TaqI TCGA 3 cut(s) 126, 135, 214
TasI AATT 8 cut(s) 367, 512, 770, 948, 965, 1021, 1059, 1084
TatI WGTACW 1 cut(s) 690
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 3 cut(s) 324, 346, 650
Tru1I TTAA 3 cut(s) 176, 968, 1131
Tru9I TTAA 3 cut(s) 176, 968, 1131
TscAI CASTG 4 cut(s) 301, 549, 678, 982
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 4 cut(s) 104, 676, 679, 1157
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 7 cut(s) 17, 332, 435, 564, 605, 885, 919
TspGWI ACGGA 2 cut(s) 539, 645
TspRI CASTG 4 cut(s) 301, 549, 678, 982
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 3 cut(s) 512, 770, 1021
XceI RCATGY 2 cut(s) 211, 1123
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.