pycom02g09330

Adipocyte plasma membrane-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000797
Physical Location & Seq
Reverse (-)
6396167 .. 6401499
5333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09330.4

Sequence Viewer

Length: 858 bp
ATGATTCGGCTGCTGACTCGGTGGTGGAGAACTGGGTTTTCACCGGCGGGAGACCGCTCGGACTCGCTCACGGCGATGACAAGGAGGTTTACGTGGCTGACACAGAAAAGGGCCATCTTTGCCTTAACATTGGGGCTACTGAAGATAAGTGAGGAGGGAATAATTGAACTGCTGACAGATGAGGCCGAGGGTGTAAAATTCAAGCTGACAGATTGTGTAGATGTAGCAGAAGATGGTATGATTTATTTCACAGATGCTTCATACAAATACAGCTTGGATGAGTTCATGTTGGACATTTTGGAAGGTAGACCCTACGGTAGATTTCTGAGCTACAATCTGATGACCAAAGAGACCAAAGTGCTCGTTCGCAACCTGTACTTTGCTAATGGAGTTGCAGTCTCTCCAGATCAAAACTATGTCATCTTCTGCGAAACTGTCATGAGAAGGTGTAGGAAATATTACCTGCAAGGCAGTAAAAAAGGGAGTGTAGAAAACTTTATCGACCAGTTACCAGGCACGCCTGATAATATCAGATATGATGGAGAAGGCCACTACTGGATTGCACTGGCCACGGATGTTAAACCATACTGGGATCATATCGCACTTCGATATCCTTTTATTCGGAAAGTCCTATCGATCGTGAAGCGGTATGCAGGAGGCAGGCCACATTTGGAGAAGAATGCTGGCGTATTTGTTGTGAATTTGGATGGGGAGCCGATTGCTCATTACTCTGATCCTGAGTTGTTTCTCATTACAAGTGGGATCAAGATTGGAAAGTACCTCTACTGTGGTTCCGTCGTCTATCCCTACATTATCCGCCTCGATCTTGATCGACATCCTGCACATGCCACCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

286

Amino Acids

32.76

Weight (kDa)

8.2

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 471
AccBSI CCGCTC 1 cut(s) 57
AccI GTMKAC 1 cut(s) 307
AciI CCGC 4 cut(s) 47, 55, 646, 817
AclWI GGATC 3 cut(s) 600, 728, 770
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 2 cut(s) 197, 700
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 2 cut(s) 377, 779
AgsI TTSAA 2 cut(s) 167, 202
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 3 cut(s) 205, 273, 330
AluI AGCT 3 cut(s) 205, 273, 330
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 3 cut(s) 45, 344, 403
AlwI GGATC 3 cut(s) 600, 728, 770
AoxI GGCC 5 cut(s) 111, 183, 547, 567, 662
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 197, 700
AspS9I GGNCC 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 33
BalI TGGCCA 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 363
BccI CCATC 4 cut(s) 122, 227, 533, 701
BceAI ACGGC 1 cut(s) 87
BciT130I CCWGG 1 cut(s) 513
BcoDI GTCTC 3 cut(s) 45, 344, 403
BfuAI ACCTGC 1 cut(s) 471
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
Bme1390I CCNGG 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 111
BmiI GGNNCC 2 cut(s) 714, 793
BmrFI CCNGG 1 cut(s) 513
BmrI ACTGGG 2 cut(s) 42, 598
BmsI GCATC 1 cut(s) 244
BmuI ACTGGG 2 cut(s) 42, 598
BpmI CTGGAG 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 93
BsaBI GATNNNNATC 2 cut(s) 828, 834
BsaI GGTCTC 2 cut(s) 45, 344
BsaJI CCNNGG 2 cut(s) 186, 570
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 5 cut(s) 37, 505, 560, 570, 593
Bse8I GATNNNNATC 2 cut(s) 828, 834
BseBI CCWGG 1 cut(s) 513
BseCI ATCGAT 1 cut(s) 635
BseDI CCNNGG 2 cut(s) 186, 570
BseGI GGATG 4 cut(s) 283, 580, 712, 835
BseJI GATNNNNATC 2 cut(s) 828, 834
BseMII CTCAG 2 cut(s) 317, 729
BseNI ACTGG 5 cut(s) 37, 505, 560, 570, 593
BseRI GAGGAG 1 cut(s) 167
BsgI GTGCAG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 639
BshFI GGCC 5 cut(s) 113, 185, 549, 569, 664
BshVI ATCGAT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 639
BsiHKAI GWGCWC 1 cut(s) 363
BsiSI CCGG 1 cut(s) 44
BsmAI GTCTC 3 cut(s) 45, 344, 403
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 5 cut(s) 113, 185, 549, 569, 664
Bso31I GGTCTC 2 cut(s) 45, 344
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
BspACI CCGC 4 cut(s) 47, 55, 646, 817
BspANI GGCC 5 cut(s) 113, 185, 549, 569, 664
BspCNI CTCAG 2 cut(s) 318, 730
BspDI ATCGAT 1 cut(s) 635
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 714, 793
BspMI ACCTGC 1 cut(s) 471
BspPI GGATC 3 cut(s) 600, 728, 770
BspTNI GGTCTC 2 cut(s) 45, 344
BsrBI CCGCTC 1 cut(s) 57
BsrFI RCCGGY 1 cut(s) 43
BsrI ACTGG 5 cut(s) 37, 505, 560, 570, 593
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 186, 570
BssMI GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 3 cut(s) 317, 436, 788
BstBAI YACGTR 1 cut(s) 93
BstC8I GCNNGC 3 cut(s) 518, 662, 685
BstDEI CTNAG 2 cut(s) 326, 738
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 4 cut(s) 283, 580, 712, 835
BstKTI GATC 7 cut(s) 409, 595, 639, 736, 765, 826, 832
BstMAI GTCTC 3 cut(s) 45, 344, 403
BstMBI GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
BstMCI CGRYCG 1 cut(s) 639
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 513
BstNSI RCATGY 1 cut(s) 848
BstSCI CCNGG 1 cut(s) 511
Bsu15I ATCGAT 1 cut(s) 635
BsuRI GGCC 5 cut(s) 113, 185, 549, 569, 664
BsuTUI ATCGAT 1 cut(s) 635
BtgI CCRYGG 1 cut(s) 570
BtgZI GCGATG 1 cut(s) 89
BtsCI GGATG 4 cut(s) 283, 580, 712, 835
BtsIMutI CAGTG 1 cut(s) 563
BveI ACCTGC 1 cut(s) 471
Cac8I GCNNGC 3 cut(s) 518, 662, 685
CciI TCATGA 1 cut(s) 438
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 111
ClaI ATCGAT 1 cut(s) 635
Csp6I GTAC 2 cut(s) 376, 778
CviAII CATG 4 cut(s) 286, 439, 845, 855
CviQI GTAC 2 cut(s) 376, 778
DdeI CTNAG 2 cut(s) 326, 738
DpnI GATC 7 cut(s) 408, 594, 638, 735, 764, 825, 831
DpnII GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
EaeI YGGCCR 1 cut(s) 567
EciI GGCGGA 1 cut(s) 806
Eco31I GGTCTC 2 cut(s) 45, 344
Eco32I GATATC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 511
EcoRV GATATC 1 cut(s) 611
FaeI CATG 4 cut(s) 289, 442, 848, 858
FatI CATG 4 cut(s) 285, 438, 844, 854
FauI CCCGC 1 cut(s) 40
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 1 cut(s) 11
FokI GGATG 4 cut(s) 290, 587, 719, 822
Fsp4HI GCNGC 1 cut(s) 11
GluI GCNGC 1 cut(s) 11
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 5 cut(s) 113, 185, 549, 569, 664
HapII CCGG 1 cut(s) 44
Hin1II CATG 4 cut(s) 289, 442, 848, 858
HinfI GANTC 3 cut(s) 4, 16, 62
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 33
Hpy166II GTNNAC 2 cut(s) 90, 308
Hpy188I TCNGA 6 cut(s) 61, 327, 339, 533, 624, 733
Hpy188III TCNNGA 6 cut(s) 404, 439, 640, 737, 766, 827
Hpy8I GTNNAC 2 cut(s) 90, 308
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 3 cut(s) 296, 438, 539
HpyCH4III ACNGT 3 cut(s) 317, 436, 788
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 5 cut(s) 395, 466, 563, 653, 842
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 326, 738
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 4 cut(s) 289, 442, 848, 858
Kzo9I GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
LmnI GCTCC 1 cut(s) 712
LweI GCATC 1 cut(s) 244
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 507
MalI GATC 7 cut(s) 408, 594, 638, 735, 764, 825, 831
MbiI CCGCTC 1 cut(s) 57
MboI GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
MboII GAAGA 4 cut(s) 154, 242, 415, 688
MhlI GDGCHC 1 cut(s) 363
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 3 cut(s) 162, 197, 700
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 2 cut(s) 10, 56
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 8 cut(s) 78, 145, 148, 175, 181, 650, 791, 830
Mox20I TGGCCA 1 cut(s) 569
MscI TGGCCA 1 cut(s) 569
MseI TTAA 2 cut(s) 125, 579
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 569
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 513
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeII GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
NlaIII CATG 4 cut(s) 289, 442, 848, 858
NlaIV GGNNCC 2 cut(s) 714, 793
NmeAIII GCCGAG 1 cut(s) 211
NspI RCATGY 1 cut(s) 848
PagI TCATGA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 71
PctI GAATGC 1 cut(s) 685
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 1 cut(s) 12
Ple19I CGATCG 1 cut(s) 639
PleI GAGTC 2 cut(s) 10, 56
PpsI GAGTC 2 cut(s) 10, 56
Ppu21I YACGTR 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 714, 793
PspPI GGNCC 1 cut(s) 111
PvuI CGATCG 1 cut(s) 639
RsaI GTAC 2 cut(s) 377, 779
RsaNI GTAC 2 cut(s) 376, 778
RseI CAYNNNNRTG 1 cut(s) 74
SaqAI TTAA 2 cut(s) 125, 579
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 7 cut(s) 406, 592, 636, 733, 762, 823, 829
Sau96I GGNCC 1 cut(s) 111
SchI GAGTC 2 cut(s) 10, 56
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 363
SfaNI GCATC 1 cut(s) 244
SgrAI CRCCGGYG 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 3 cut(s) 162, 197, 700
SsiI CCGC 4 cut(s) 47, 55, 646, 817
SspI AATATT 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 511
TaaI ACNGT 3 cut(s) 317, 436, 788
TaiI ACGT 1 cut(s) 95
TaqI TCGA 5 cut(s) 501, 607, 635, 822, 832
TasI AATT 3 cut(s) 162, 197, 700
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 125, 579
Tru9I TTAA 2 cut(s) 125, 579
TscAI CASTG 1 cut(s) 570
TseI GCWGC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 249, 274
TspGWI ACGGA 2 cut(s) 587, 784
TspRI CASTG 1 cut(s) 570
XapI RAATTY 2 cut(s) 197, 700
XceI RCATGY 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.