Rroxscaffold_2G00144630

Adipocyte plasma membrane-associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000797
Physical Location & Seq
Forward (+)
82541120 .. 82552454
11335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144630.1

Sequence Viewer

Length: 1026 bp
ATGCCCAAGTCAAGCAACCAAGACTCGCCTTCCTCTCGCAACTCAATCTACCGAAGATCATCATCATCATCATGGCCCTTAAACATTCTCTTCTTCTCAATACCGGCCGGCGGGCGCCGCCGTGGTCCTCACTTGACTCATTCGACCCGGCCTCTCGCTTCTTCGCGAGTTGATGGGGCCGAAGACGTTGCTTATGATTCAAAGTCGGGACTCGTTTACACGGGATGTGCGGACGGGTGGATCAAGCGAGTCAAGTTGAACGAGTCGGCCGTCGACTCGGTGGTGGAGAATCGGGTTAGTACCGGCGGGAGACCATTCGGACTCGCACACGGTCATAACGGAGAGCTGTTGGTGGCGGACGGGGAAAGGGGACTACTGAGAATAGACGAGGATAGAGCAGTGGAGCTGCTGACAGATGAGGCTGAGGGTGTAAAATTCAAGCTAACAAACTGTGTCGATGTAGCGAAAGATGGCATGATTTACTTCACAGATGCTTCATATAAAAACAGCGTAGAAGACTTCATCTGGGAGTTTCTAGAGGGCAGACCCCATGGTAGGTTACTGAGCTACAATCCATCGACCAAAGAGACCAAAGTGCTTGTCCGCGATCTCTACTTTGCTAATGGAGTAGCAATCTCTCCGGATCAGAATTATGTGATCTTCTGTGAAACTTTCGTGAAAAGATGTAGGAAGTACCACCTGCAAGGCGAGAAGAAAGGGAGCATAGAGAACTTCATTGACAACTTGCCGGGCTTCCCGGATAATATCCGATATGATGGGGAAGGCCATTACTGGATTGCAATACCTACGGAGAAGTATTCTGGTGTAATGGCTGTGAATTTGGAAGGCGAACCTACTGCCCACTATTCTGATCCAGATTTATCACTCATTACAAGTGGGATCAAGATCGGAAACTACCTCTATTGCGGTGGAAATGAGTCTTCAAAGCAAAGCAGGAGTGAGAAGAAGAGCCATAAGGCAATGCTGAAGCTGGGTATGAAGAAAGTCACTGGTGTTAGCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Str_synth

Protein Analysis

341

Amino Acids

37.79

Weight (kDa)

7.65

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SGL PF08450 101 - 298 2e-16 SMP-30/Gluconolactonase/LRE-like region
Str_synth PF03088 150 - 236 7.6e-31 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 708
Acc36I ACCTGC 1 cut(s) 708
AccB1I GGYRCC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 273
AccII CGCG 2 cut(s) 166, 606
AccIII TCCGGA 1 cut(s) 640
AciI CCGC 7 cut(s) 111, 118, 230, 306, 356, 604, 927
AclWI GGATC 4 cut(s) 248, 651, 866, 908
AcoI YGGCCR 2 cut(s) 105, 267
AcsI RAATTY 2 cut(s) 434, 838
AcuI CTGAAG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 115
AfaI GTAC 2 cut(s) 301, 695
AfiI CCNNNNNNNGG 2 cut(s) 110, 555
AgsI TTSAA 4 cut(s) 201, 259, 439, 945
AluBI AGCT 5 cut(s) 346, 406, 442, 567, 991
AluI AGCT 5 cut(s) 346, 406, 442, 567, 991
Alw26I GTCTC 2 cut(s) 304, 581
AlwI GGATC 4 cut(s) 248, 651, 866, 908
Aor13HI TCCGGA 1 cut(s) 640
AoxI GGCC 6 cut(s) 74, 105, 149, 177, 267, 784
ApeKI GCWGC 1 cut(s) 406
ApoI RAATTY 2 cut(s) 434, 838
ArsI GACNNNNNNTTYG 2 cut(s) 585, 617
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 3 cut(s) 75, 125, 177
AsuC2I CCSGG 3 cut(s) 148, 750, 758
AvaII GGWCC 1 cut(s) 125
BanI GGYRCC 1 cut(s) 114
BbsI GAAGAC 3 cut(s) 189, 522, 933
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 1 cut(s) 393
BccI CCATC 4 cut(s) 167, 464, 583, 770
BceAI ACGGC 2 cut(s) 105, 254
BcgI CGANNNNNNTGC 4 cut(s) 170, 204, 839, 873
BcnI CCSGG 3 cut(s) 148, 750, 758
BcoDI GTCTC 2 cut(s) 304, 581
BfaI CTAG 1 cut(s) 536
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 708
BisI GCNGC 2 cut(s) 118, 407
BlsI GCNGC 2 cut(s) 119, 408
Bme1390I CCNGG 3 cut(s) 148, 750, 758
Bme18I GGWCC 1 cut(s) 125
BmgT120I GGNCC 3 cut(s) 75, 125, 177
BmiI GGNNCC 2 cut(s) 116, 178
BmrFI CCNGG 3 cut(s) 148, 750, 758
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 3 cut(s) 189, 522, 933
Bpu10I CCTNAGC 1 cut(s) 423
BpuMI CCSGG 3 cut(s) 148, 750, 758
BsaBI GATNNNNATC 2 cut(s) 61, 905
BsaHI GRCGYC 1 cut(s) 115
BsaI GGTCTC 2 cut(s) 304, 581
BsaJI CCNNGG 2 cut(s) 121, 550
BsaWI WCCGGW 1 cut(s) 640
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 555
Bse118I RCCGGY 3 cut(s) 103, 107, 302
Bse1I ACTGG 2 cut(s) 797, 1015
Bse3DI GCAATG 1 cut(s) 987
Bse8I GATNNNNATC 2 cut(s) 61, 905
BseAI TCCGGA 1 cut(s) 640
BseDI CCNNGG 2 cut(s) 121, 550
BseGI GGATG 1 cut(s) 230
BseJI GATNNNNATC 2 cut(s) 61, 905
BseLI CCNNNNNNNGG 2 cut(s) 110, 555
BseMI GCAATG 1 cut(s) 987
BseMII CTCAG 3 cut(s) 368, 414, 554
BseNI ACTGG 2 cut(s) 797, 1015
BseX3I CGGCCG 2 cut(s) 105, 267
BseXI GCAGC 1 cut(s) 393
BseYI CCCAGC 1 cut(s) 991
Bsh1236I CGCG 2 cut(s) 166, 606
Bsh1285I CGRYCG 2 cut(s) 108, 270
BshFI GGCC 6 cut(s) 76, 107, 151, 179, 269, 786
BshNI GGYRCC 1 cut(s) 114
BsiEI CGRYCG 2 cut(s) 108, 270
BsiSI CCGG 7 cut(s) 104, 108, 148, 303, 641, 749, 758
BslFI GGGAC 2 cut(s) 222, 384
BslI CCNNNNNNNGG 2 cut(s) 110, 555
BsmAI GTCTC 2 cut(s) 304, 581
BsmFI GGGAC 2 cut(s) 222, 384
BsnI GGCC 6 cut(s) 76, 107, 151, 179, 269, 786
Bso31I GGTCTC 2 cut(s) 304, 581
Bsp13I TCCGGA 1 cut(s) 640
Bsp143I GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
Bsp19I CCATGG 1 cut(s) 550
Bsp68I TCGCGA 1 cut(s) 166
BspACI CCGC 7 cut(s) 111, 118, 230, 306, 356, 604, 927
BspANI GGCC 6 cut(s) 76, 107, 151, 179, 269, 786
BspCNI CTCAG 3 cut(s) 369, 415, 555
BspEI TCCGGA 1 cut(s) 640
BspFNI CGCG 2 cut(s) 166, 606
BspLI GGNNCC 2 cut(s) 116, 178
BspMI ACCTGC 1 cut(s) 708
BspPI GGATC 4 cut(s) 248, 651, 866, 908
BspQI GCTCTTC 1 cut(s) 962
BspT107I GGYRCC 1 cut(s) 114
BspTNI GGTCTC 2 cut(s) 304, 581
BsrDI GCAATG 1 cut(s) 987
BsrFI RCCGGY 3 cut(s) 103, 107, 302
BsrI ACTGG 2 cut(s) 797, 1015
BssAI RCCGGY 3 cut(s) 103, 107, 302
BssECI CCNNGG 2 cut(s) 121, 550
BssMI GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
BssNI GRCGYC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 550
Bst4CI ACNGT 2 cut(s) 332, 452
Bst6I CTCTTC 2 cut(s) 95, 962
BstACI GRCGYC 1 cut(s) 115
BstC8I GCNNGC 2 cut(s) 109, 113
BstDEI CTNAG 4 cut(s) 377, 423, 563, 1023
BstDSI CCRYGG 2 cut(s) 121, 550
BstF5I GGATG 1 cut(s) 230
BstFNI CGCG 2 cut(s) 166, 606
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 8 cut(s) 59, 243, 610, 646, 660, 874, 903, 909
BstMAI GTCTC 2 cut(s) 304, 581
BstMBI GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
BstMCI CGRYCG 2 cut(s) 108, 270
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstSCI CCNGG 3 cut(s) 146, 748, 756
BstUI CGCG 2 cut(s) 166, 606
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 3 cut(s) 189, 522, 933
BstZI CGGCCG 2 cut(s) 105, 267
BsuRI GGCC 6 cut(s) 76, 107, 151, 179, 269, 786
BtgI CCRYGG 2 cut(s) 121, 550
BtsCI GGATG 1 cut(s) 230
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 405, 1008
BtuMI TCGCGA 1 cut(s) 166
BveI ACCTGC 1 cut(s) 708
Cac8I GCNNGC 2 cut(s) 109, 113
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 3 cut(s) 103, 107, 302
Cfr13I GGNCC 3 cut(s) 75, 125, 177
Csp6I GTAC 2 cut(s) 300, 694
CviAII CATG 3 cut(s) 72, 475, 551
CviQI GTAC 2 cut(s) 300, 694
DdeI CTNAG 4 cut(s) 377, 423, 563, 1023
DinI GGCGCC 1 cut(s) 116
DpnI GATC 8 cut(s) 58, 242, 609, 645, 659, 873, 902, 908
DpnII GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
EaeI YGGCCR 2 cut(s) 105, 267
EagI CGGCCG 2 cut(s) 105, 267
Eam1104I CTCTTC 2 cut(s) 95, 962
EarI CTCTTC 2 cut(s) 95, 962
EciI GGCGGA 1 cut(s) 371
EclXI CGGCCG 2 cut(s) 105, 267
Eco130I CCWWGG 1 cut(s) 550
Eco31I GGTCTC 2 cut(s) 304, 581
Eco47I GGWCC 1 cut(s) 125
Eco52I CGGCCG 2 cut(s) 105, 267
Eco57I CTGAAG 1 cut(s) 1007
EcoT14I CCWWGG 1 cut(s) 550
EgeI GGCGCC 1 cut(s) 116
EheI GGCGCC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 550
FaeI CATG 3 cut(s) 75, 478, 554
FaqI GGGAC 2 cut(s) 222, 384
FatI CATG 3 cut(s) 71, 474, 550
FauI CCCGC 2 cut(s) 104, 299
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 2 cut(s) 118, 407
FokI GGATG 1 cut(s) 237
Fsp4HI GCNGC 2 cut(s) 118, 407
FspBI CTAG 1 cut(s) 536
GlaI GCGC 1 cut(s) 116
GluI GCNGC 2 cut(s) 118, 407
GsaI CCCAGC 1 cut(s) 995
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 6 cut(s) 76, 107, 151, 179, 269, 786
HapII CCGG 7 cut(s) 104, 108, 148, 303, 641, 749, 758
HhaI GCGC 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 3 cut(s) 75, 478, 554
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HpaII CCGG 7 cut(s) 104, 108, 148, 303, 641, 749, 758
Hpy166II GTNNAC 2 cut(s) 217, 274
Hpy188I TCNGA 5 cut(s) 320, 648, 770, 871, 911
Hpy188III TCNNGA 7 cut(s) 165, 207, 536, 641, 676, 875, 904
Hpy8I GTNNAC 2 cut(s) 217, 274
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 39, 776, 839
HpyCH4III ACNGT 2 cut(s) 332, 452
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 703, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 4 cut(s) 377, 423, 563, 1023
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 3 cut(s) 75, 478, 554
HspAI GCGC 1 cut(s) 115
KasI GGCGCC 1 cut(s) 114
Kpn2I TCCGGA 1 cut(s) 640
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Kzo9I GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
LguI GCTCTTC 1 cut(s) 962
LmnI GCTCC 2 cut(s) 403, 720
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 558, 1006
MalI GATC 8 cut(s) 58, 242, 609, 645, 659, 873, 902, 908
MboI GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
MluCI AATT 3 cut(s) 434, 649, 838
Mly113I GGCGCC 1 cut(s) 115
MlyI GAGTC 8 cut(s) 17, 130, 204, 258, 269, 272, 315, 947
MnlI CCTC 8 cut(s) 43, 138, 162, 382, 412, 418, 532, 929
MroI TCCGGA 1 cut(s) 640
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 1 cut(s) 80
MslI CAYNNNNRTG 1 cut(s) 70
MspI CCGG 7 cut(s) 104, 108, 148, 303, 641, 749, 758
MspR9I CCNGG 3 cut(s) 148, 750, 758
MvnI CGCG 2 cut(s) 166, 606
MwoI GCNNNNNNNGC 1 cut(s) 117
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 115
NciI CCSGG 3 cut(s) 148, 750, 758
NcoI CCATGG 1 cut(s) 550
NdeII GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 3 cut(s) 75, 478, 554
NlaIV GGNNCC 2 cut(s) 116, 178
NmuCI GTSAC 1 cut(s) 1006
NruI TCGCGA 1 cut(s) 166
PaqCI CACCTGC 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 962
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 2 cut(s) 197, 289
PfoI TCCNGGA 1 cut(s) 756
PkrI GCNGC 2 cut(s) 119, 408
PleI GAGTC 8 cut(s) 17, 130, 204, 257, 269, 271, 315, 946
PluTI GGCGCC 1 cut(s) 118
PpsI GAGTC 8 cut(s) 17, 130, 204, 257, 269, 271, 315, 946
PspFI CCCAGC 1 cut(s) 991
PspN4I GGNNCC 2 cut(s) 116, 178
PspPI GGNCC 3 cut(s) 75, 125, 177
RruI TCGCGA 1 cut(s) 166
RsaI GTAC 2 cut(s) 301, 695
RsaNI GTAC 2 cut(s) 300, 694
RseI CAYNNNNRTG 1 cut(s) 70
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 962
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 2 cut(s) 118, 407
Sau3AI GATC 8 cut(s) 56, 240, 607, 643, 657, 871, 900, 906
Sau96I GGNCC 3 cut(s) 75, 125, 177
SchI GAGTC 8 cut(s) 17, 130, 204, 258, 269, 272, 315, 947
ScrFI CCNGG 3 cut(s) 148, 750, 758
SfaNI GCATC 1 cut(s) 481
SfoI GGCGCC 1 cut(s) 116
SinI GGWCC 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 70
Sse9I AATT 3 cut(s) 434, 649, 838
SsiI CCGC 7 cut(s) 111, 118, 230, 306, 356, 604, 927
SspDI GGCGCC 1 cut(s) 114
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 3 cut(s) 146, 748, 756
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 2 cut(s) 332, 452
TaiI ACGT 1 cut(s) 189
TaqI TCGA 4 cut(s) 143, 273, 456, 578
TasI AATT 3 cut(s) 434, 649, 838
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 2 cut(s) 197, 289
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TscAI CASTG 2 cut(s) 405, 1015
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 4 cut(s) 486, 511, 724, 1013
TspGWI ACGGA 2 cut(s) 354, 824
TspRI CASTG 2 cut(s) 405, 1015
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 2 cut(s) 434, 838
XbaI TCTAGA 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.