Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000962

Physical Location & Seq

Genomic Coordinates Reverse (-)

31718839 .. 31728839

Gene Structure 10001 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1262900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3819 bp

ATGAATAGGGCATCGCATATGAGTCATCCCAATCTGGGTCAAGATTTCTTCGCCCAGACGCCTTTTAAAGCTAATTTGTTGGAACATGTCAATGCACTTAATAATTTCTCGTGCCCGCATAACATTCTGAGCTCAAATTTCATTTTCTCCTTACCAACACAAAAACCTCATGCCGAAGAGGCAATGGGTTCCGGCTCAATGGCTTGTCCAATTCAGAATACTGAAAGATTACAGAGTGCACAAGTTCAAAAGGCTTGGCATGCTCTTTCCAATCTTCAGATTTCTTGTAGGAACTACACAAAACCTGGAAAAACTCTTCTAGTTAAGGATGTTAATGCGGAGTGTCGTCGTGATGTTGGAACAACAACTTTGCCAAGCTCATGCAACATTGATAAGAGTCGCGCACATATGCCAACACATCAATACATTAATGGAACTAATACCAGGAACAGTGAAGCAGCAATTTGCTCGGGCAATCCGTTTCCGTCAAGTAATGTTAATGTTGCGGATGCAGGGAAAAATCTTGGACGGCAAAGTCAGTTCAGAGCAACAATCGTCAACAGTTCTGATGATCGGGCTTTGGATGGATCTCTTGGCAACAATAGTGTTCATAACAGCCAAGGGAAAGACTCTGCAAAAGTTTTTGTTAATGACCTGGACGACGATGATGATGACATAATTGAGAATATTGATGTTGACCAGATAGTGGAACAGTACCAATCAAGTTGCACACCTCAACCATCAATTTCCAAACTTCCACCCATTGCTCCCTTTATAGATAAAGATAATATTGCAGGACAAGATGCTACTAGTTTGCCACCGGAATTGTGTTCAAATTGCAGTCATGGGTTTAAGATAGGACTTTGCCCAGAAGCTGCAAGTCATTTGCAGGAAATGAAGGATACCCTAATTATCATATCAAATGAACTGCTTGATGATGTCAATGACCTCAGTCCAGAACAAATAAAAAAGCTTCGCCAAGATAGGTTACAGCTAAATAAGCAGATTCAGCAGCTTGAGAGATATCTTTGTAACAACTCATTGGACGAAGAAAGGCGAAAGTCACATTTTTCTGCATCAACAGCAACTCCCAGGCCTTTCCAATGTGAAACACCTCAAGCAGCTGCATTCAGGACTGATCCCATGAGATTTGATTCACAGGTTCATCCACATAATGAGTCTGGAGGATATGACAGATGTAATTTATCATCAGTTTCATTCTCTTCTGTTGATAGGCTTGATTTCTCATCTTGTCCTGTTGAGAGGGAACCATACATTTCAAAGTTTGTTGAAGTTAACTACATTGAAGGTTCTAAGGACAAAAAATGGAGTAGTGATAATTTTCCATGGACCAAAAAGCTGGAGGATGGTAACAAGAAAGTGTTTGGAAATCACTCTTTCCGGCTCAACCAAAGAGAGGTCATTAATGCTACGATGAGTGGCTATGATGTTTTTGTTTTAATGCCAACTGGAGGGGGTAAGAGCTTGACATATCAGGTAAAAGAATTATTTTTTGGAACCTCTTACGAGATACAGTCATTTCTGTTTAAGCTCCCTGCGCTAATTTGTCCGGGAATAACATTGGTTATTTCTCCCCTTGTGTCACTTATTCAAGATCAAATAATGCATTTATTACAGGCAAATGTTCCTGCTGCTTACCTAAGTGCCAATATGGAGTGGTCTGAACAACAGGAAATACTTAGAGAACTAAATTATGAGTATTGTAAATACAAGCTGTTATATGTCACCCCAGAGAAAGTTGCTAAAAGTGATGCTCTTTTACGGCAGTTGGAGAATTTAAATGCTCGTCAATTGCTTTCTAGGATTGTTATTGATGAAGCTCATTGTGTGAGTCAGTGGGGGCATGATTTCAGACCAGATTATCAGGCTCTTGGTATCTTGAAACAGAAGTTCCCAAATACTCCAGTTTTGGCTTTAACAGCTACTGCAACCGCTAGTGTAAAAGAAGATGTTGTGCAAGCTCTTGGTCTTGTTAACTGCATTGTTTTCCGGCAAAGTTTTAATCGCCCAAATTTACGGCAAGTTTTAAACAAAGAATCTGTTTTACAATGTTCAAATCATACTTTTTCTGAACTGTCATTTGAAGTTGAAATGATAATTCGTTCTGTGAATTGCAGGTATTCTGTTGTTCCCAAGACTAAGAAGTGTGTGGATGACATTGACAAGTTCATTAAAGAAAATCATTATGATGAAAGTGGAATAATTTATTGTCTCTCAAGAATGGACTGTGAAAAAGTTGCCGAAAGATTACAGGAATGTGGCCATAAAGCAGCATTTTACCATGGTAATATGGATCCTGCTCAGCGTGCCTTTGTTCAGAAGCAGTGGAGTAAAGATGAAATCAACGTTATATGTGCTACAGTGGCATTTGGAATGGGTATCAACAAGCCAGATGTCCGCTTTGTAATCCATCACTCTCTCCCGAAGTCTATTGAAGGCTACCATCAGGAGTGTGGTCGAGCTGGTAGAGATGGTCAGCATTCATCTTGTGTGTTGTATTACAGTTACAGTGACTATATTCGAGTAAAGCATATGATTAGTCAAGGAGTTATAGAGCAAAGCCCACTGACATCTGGATACAATCGTATCAATACTGCTAATTCTGGGAGAGTACTGGAAACGAATACCGAAAATCTTTTGCGCATGGTCAGTTATTGTGAAAATGATGTTGATTGCCGACGACTTCTACAACTTATTCATTTTGGAGAAAACTTTGATTGTGCAACATGCAAAAAGACGTGTGATAATTGTTTGAAGATTAAAAGTTTCAGTGAGAAGGATGTCACTGAAATCGCAAAGCAACTGGTTGAACTGGTGAAGTTAACAGGGCAGCAGTTTTCGTCATCTCATATTCTAGAAGTCTACAGGGGCTCCTTAAACCAATTTGTCAAGAAACACAGGCATCAGACTGTGAGACTGCATGGAGCAGGAAAACATCTAGCCAAGGGTGAAGCTTCTCGTGTATTACGTCATCTTGTTACCGAGGACCTTCTTGTTGAGGAAGTCAAGAAAAGTGATGTTTATGGATCTGTATCGTCAATACTAAAGGTTAATGAACTGAAAGCGCGTGAACTTTTCTCTGGCAGGAAGACAATTTTAATAAGATTCCCTTCCACCTTAAAAGCTTCCAAACAGAAAAACCCCGAAGCGATTTCAGCTAAAGGCTCACTGACATCTGGGATGCAGAGCCCTCCAATTACTGGCACTGAGCAACCTCAATCAAAAGGAGATTTGGTTCTATACACGAAAATATTTACTGCTTTGAAAATGCTTCGAACTAATATCATTAGAGAAGCTGGTGATGGGGTCATGGCACACCACATATTTCCGAATACAACTATTAAAAACATAAGCAGCAGAATGCCTAGAACAAAGGATGAACTCCTCGAGATAAATGGCGTTACCCAAATTAAGGTGGCCAGGTACGGAGATCAAATACTTGAAACAATTGAAGCTGCCGTAAAGGAATACTTCAAAACAGATAAAAACAGCAGCGGTAGCAATGATAGCAACGATATAAAGAGGAAAAGAGATGGTAACAAAGATCGGAAGGAAATTTTTGAAGATGACGACTTCAGAAGCACCGATAGATCGAAGAAAAGGGCTCTAAAGACGCAGAGCAATACTATTGAGACCTTCCGTTATGTGGAGCCAGATTACAGTGAATTCATAGATGATGAATTAGACTTATCTGGTTATGACTTTGAAGCTAATGTTCCGGATATGAAAACCAATCAGAAGGGTGGCGGAAGAGTGCTGCCACAGTGGTCAACTCCTGGAAATGGGCGACATCGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009266 GO:0009378 GO:0009409 GO:0009506 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032392 GO:0032508 GO:0032870 GO:0033554 GO:0033993 GO:0034641 GO:0042221 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0055044 GO:0070035 GO:0070417 GO:0070887 GO:0071103 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097305 GO:0097306 GO:0140097 GO:1901360 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko03018 ko03440 ko03460 map03018 map03440 map03460

Pfam Domains

Protein Families

DEAD HRDC Helicase_C RQC RecQ_Zn_bind

Protein Analysis

1273

Amino Acids

143.22

Weight (kDa)

7.34

Isoelectric Point (pI)

45.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	470 - 655	1.4e-20	DEAD/DEAH box helicase
Helicase_C	PF00271	725 - 832	2.3e-19	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	843 - 925	9.2e-15	RecQ zinc-binding
RQC	PF09382	931 - 1046	3.7e-21	RQC domain
HRDC	PF00570	1090 - 1157	6e-14	HRDC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000962)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10930
fragaria_vesca	FvH4_5g28920 FvH4_5g28920
malus_domestica	MD02G1262900.v1.1 MD07G1056800.v1.1 MD07G1057400.v1.1
prunus_persica	Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1
pyrus_communis	pycom02g22480 pycom02g22490 pycom02g22530 pycom07g04380
rosa_chinensis	RchiOBHm_Chr2g0101731 RchiOBHm_Chr2g0146651 RchiOBHm_Chr3g0471841 RchiOBHm_Chr4g0402091 RchiOBHm_Chr5g0019321 RchiOBHm_Chr7g0221231
rosa_laevigata	RLG00000002168 RLG00000008436
rosa_multiflora	Rmu_co8338979.1_g000001 Rmu_co8389917.1_g000001 Rmu_sc0000212.1_g000013 Rmu_sc0000324.1_g000016 Rmu_sc0000518.1_g000001 Rmu_sc0000808.1_g000043 Rmu_sc0002206.1_g000018 Rmu_sc0002551.1_g000024 Rmu_sc0004296.1_g000023 Rmu_sc0009806.1_g000017 Rmu_sc0032411.1_g000001 Rmu_ssc0000291.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431210 Rroxscaffold_1G00026280 Rroxscaffold_3G00238540 Rroxscaffold_4G00313160 Rroxscaffold_4G00330040 Rroxscaffold_5G00342230 Rroxscaffold_5G00352050
rosa_rugosa	Rorug07G0201600 Rorug07G0253300
rosa_samantha	Rh1BG131100 Rh1CG101400 Rh2BG215600 Rh2CG271000 Rh4DG065200 Rh4DG134600 Rh5AG275200 Rh5AG434000 Rh7AG340500 Rh7BG172400 Rh7BG331600 Rh7BG331700 Rh7CG358400 Rh7DG335700 Rh7DG335800 Rh7DG339200 Rh7DG339300
rosa_wichuraiana	Rw6G023870 Rw7G028980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	534
Acc16I	TGCGCA	1 cut(s)	2663
Acc36I	ACCTGC	1 cut(s)	2127
AccB7I	CCANNNNNTGG	2 cut(s)	706, 3221
AccI	GTMKAC	1 cut(s)	2883
AccII	CGCG	2 cut(s)	402, 3088
AccIII	TCCGGA	1 cut(s)	3739
AciI	CCGC	7 cut(s)	116, 338, 506, 1953, 2419, 3516, 3768
AclI	AACGTT	1 cut(s)	2367
AclWI	GGATC	5 cut(s)	595, 1133, 2309, 2322, 3055
AcoI	YGGCCR	2 cut(s)	2281, 3438
AcsI	RAATTY	5 cut(s)	136, 1795, 2032, 3576, 3686
AcuI	CTGAAG	2 cut(s)	260, 3580
AcyI	GRCGYC	1 cut(s)	59
AfaI	GTAC	3 cut(s)	716, 2634, 3446
AfiI	CCNNNNNNNGG	8 cut(s)	35, 706, 1417, 1472, 3221, 3433, 3667, 3803
AflIII	ACRYGT	2 cut(s)	85, 2759
AhlI	ACTAGT	1 cut(s)	809
AjiI	CACGTC	1 cut(s)	2760
AjnI	CCWGG	6 cut(s)	304, 443, 654, 1091, 3440, 3796
AjuI	GAANNNNNNNTTGG	2 cut(s)	1497, 1529
AloI	GAACNNNNNNTCC	2 cut(s)	3240, 3272
Alw21I	GWGCWC	2 cut(s)	134, 241
Alw26I	GTCTC	3 cut(s)	2237, 2929, 3647
Alw44I	GTGCAC	1 cut(s)	237
AlwI	GGATC	5 cut(s)	595, 1133, 2309, 2322, 3055
AlwNI	CAGNNNCTG	2 cut(s)	875, 1946
Ama87I	CYCGRG	2 cut(s)	469, 3407
Aor13HI	TCCGGA	1 cut(s)	3739
AoxI	GGCC	3 cut(s)	1094, 2281, 3438
ApaLI	GTGCAC	1 cut(s)	237
ApoI	RAATTY	5 cut(s)	136, 1795, 2032, 3576, 3686
AseI	ATTAAT	2 cut(s)	429, 1425
AspLEI	GCGC	4 cut(s)	404, 1561, 2664, 3088
AspS9I	GGNCC	2 cut(s)	1350, 3007
AsuC2I	CCSGG	1 cut(s)	1572
AsuHPI	GGTGA	4 cut(s)	1738, 2848, 2981, 3332
AsuII	TTCGAA	1 cut(s)	3295
AvaI	CYCGRG	2 cut(s)	469, 3407
AvaII	GGWCC	2 cut(s)	1350, 3007
BaeGI	GKGCMC	2 cut(s)	116, 241
BaeI	ACNNNNGTAYC	4 cut(s)	2590, 2590, 2623, 2623
BalI	TGGCCA	2 cut(s)	2283, 3440
BamHI	GGATCC	1 cut(s)	2314
BanII	GRGCYC	4 cut(s)	134, 2894, 3212, 3628
BauI	CACGAG	2 cut(s)	109, 2979
BbsI	GAAGAC	1 cut(s)	3116
Bbv12I	GWGCWC	2 cut(s)	134, 241
BccI	CCATC	8 cut(s)	578, 748, 1361, 2439, 2472, 2486, 3317, 3548
BceAI	ACGGC	4 cut(s)	545, 1799, 2054, 3464
BcgI	CGANNNNNNTGC	2 cut(s)	450, 484
BciT130I	CCWGG	6 cut(s)	306, 445, 656, 1093, 3442, 3798
BciVI	GTATCC	2 cut(s)	895, 2591
BcnI	CCSGG	1 cut(s)	1572
BcoDI	GTCTC	3 cut(s)	2237, 2929, 3647
BcuI	ACTAGT	1 cut(s)	809
BfaI	CTAG	7 cut(s)	320, 810, 1821, 1956, 2876, 2960, 3387
BfmI	CTRYAG	2 cut(s)	2379, 2884
BfuAI	ACCTGC	1 cut(s)	2127
BfuI	GTATCC	2 cut(s)	895, 2591
BglI	GCCNNNNNGGC	1 cut(s)	179
BlpI	GCTNAGC	1 cut(s)	2322
BmcAI	AGTACT	1 cut(s)	2634
Bme1390I	CCNGG	7 cut(s)	306, 445, 656, 1093, 1572, 3442, 3798
Bme18I	GGWCC	2 cut(s)	1350, 3007
BmeT110I	CYCGRG	2 cut(s)	469, 3407
BmgBI	CACGTC	1 cut(s)	2760
BmgT120I	GGNCC	2 cut(s)	1350, 3007
BmiI	GGNNCC	6 cut(s)	190, 1269, 1519, 2316, 2893, 3672
BmrFI	CCNGG	7 cut(s)	306, 445, 656, 1093, 1572, 3442, 3798
BmsI	GCATC	7 cut(s)	20, 499, 793, 1085, 1762, 2932, 3192
BoxI	GACNNNNGTC	1 cut(s)	951
BpiI	GAAGAC	1 cut(s)	3116
BpmI	CTGGAG	4 cut(s)	1203, 1382, 1491, 1908
Bpu1102I	GCTNAGC	1 cut(s)	2322
Bpu14I	TTCGAA	1 cut(s)	3295
BpuEI	CTTGAG	3 cut(s)	1037, 1101, 2221
BpuMI	CCSGG	1 cut(s)	1572
BsaBI	GATNNNNATC	1 cut(s)	3052
BsaHI	GRCGYC	1 cut(s)	59
BsaI	GGTCTC	1 cut(s)	3647
BsaJI	CCNNGG	6 cut(s)	619, 1091, 1346, 2302, 2964, 3003
BsaWI	WCCGGW	2 cut(s)	820, 3739
BsaXI	ACNNNNNCTCC	6 cut(s)	1072, 1102, 2876, 2906, 3240, 3270
Bsc4I	CCNNNNNNNGG	8 cut(s)	35, 706, 1417, 1472, 3221, 3433, 3667, 3803
Bse1I	ACTGG	6 cut(s)	1474, 1925, 2640, 2829, 2838, 3226
Bse3DI	GCAATG	3 cut(s)	189, 762, 3529
Bse8I	GATNNNNATC	1 cut(s)	3052
BseAI	TCCGGA	1 cut(s)	3739
BseBI	CCWGG	6 cut(s)	306, 445, 656, 1093, 3442, 3798
BseDI	CCNNGG	6 cut(s)	619, 1091, 1346, 2302, 2964, 3003
BseJI	GATNNNNATC	1 cut(s)	3052
BseLI	CCNNNNNNNGG	8 cut(s)	35, 706, 1417, 1472, 3221, 3433, 3667, 3803
BseMI	GCAATG	3 cut(s)	189, 762, 3529
BseMII	CTCAG	4 cut(s)	119, 964, 2336, 3219
BseNI	ACTGG	6 cut(s)	1474, 1925, 2640, 2829, 2838, 3226
BseRI	GAGGAG	1 cut(s)	3395
BseSI	GKGCMC	2 cut(s)	116, 241
Bsh1236I	CGCG	2 cut(s)	402, 3088
BshFI	GGCC	3 cut(s)	1096, 2283, 3440
BsiHKAI	GWGCWC	2 cut(s)	134, 241
BsiHKCI	CYCGRG	2 cut(s)	469, 3407
BsiSI	CCGG	6 cut(s)	192, 821, 1402, 1571, 2011, 3740
BslI	CCNNNNNNNGG	8 cut(s)	35, 706, 1417, 1472, 3221, 3433, 3667, 3803
BsmAI	GTCTC	3 cut(s)	2237, 2929, 3647
BsmI	GAATGC	3 cut(s)	1127, 2500, 3387
BsnI	GGCC	3 cut(s)	1096, 2283, 3440
Bso31I	GGTCTC	1 cut(s)	3647
BsoBI	CYCGRG	2 cut(s)	469, 3407
Bsp119I	TTCGAA	1 cut(s)	3295
Bsp1286I	GDGCHC	6 cut(s)	116, 134, 241, 2894, 3212, 3628
Bsp13I	TCCGGA	1 cut(s)	3739
Bsp143I	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
Bsp1720I	GCTNAGC	1 cut(s)	2322
Bsp19I	CCATGG	2 cut(s)	1346, 2302
BspACI	CCGC	7 cut(s)	116, 338, 506, 1953, 2419, 3516, 3768
BspANI	GGCC	3 cut(s)	1096, 2283, 3440
BspCNI	CTCAG	4 cut(s)	120, 963, 2335, 3220
BspEI	TCCGGA	1 cut(s)	3739
BspFNI	CGCG	2 cut(s)	402, 3088
BspLI	GGNNCC	6 cut(s)	190, 1269, 1519, 2316, 2893, 3672
BspMI	ACCTGC	1 cut(s)	2127
BspPI	GGATC	5 cut(s)	595, 1133, 2309, 2322, 3055
BspT104I	TTCGAA	1 cut(s)	3295
BspTNI	GGTCTC	1 cut(s)	3647
BsrDI	GCAATG	3 cut(s)	189, 762, 3529
BsrI	ACTGG	6 cut(s)	1474, 1925, 2640, 2829, 2838, 3226
BssECI	CCNNGG	6 cut(s)	619, 1091, 1346, 2302, 2964, 3003
BssMI	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	2 cut(s)	109, 2979
BssT1I	CCWWGG	4 cut(s)	619, 1346, 2302, 2964
Bst2BI	CACGAG	2 cut(s)	109, 2979
Bst2UI	CCWGG	6 cut(s)	306, 445, 656, 1093, 3442, 3798
Bst6I	CTCTTC	4 cut(s)	171, 321, 1228, 3766
BstACI	GRCGYC	1 cut(s)	59
BstBI	TTCGAA	1 cut(s)	3295
BstC8I	GCNNGC	4 cut(s)	116, 261, 1980, 2328
BstDEI	CTNAG	8 cut(s)	128, 950, 1314, 1661, 1700, 2160, 2322, 3228
BstDSI	CCRYGG	2 cut(s)	1346, 2302
BstFNI	CGCG	2 cut(s)	402, 3088
BstHHI	GCGC	4 cut(s)	404, 1561, 2664, 3088
BstKTI	GATC	9 cut(s)	574, 590, 1141, 1618, 2317, 3050, 3454, 3568, 3614
BstMAI	GTCTC	3 cut(s)	2237, 2929, 3647
BstMBI	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
BstNI	CCWGG	6 cut(s)	306, 445, 656, 1093, 3442, 3798
BstNSI	RCATGY	3 cut(s)	89, 263, 2751
BstPAI	GACNNNNGTC	1 cut(s)	951
BstSCI	CCNGG	7 cut(s)	304, 443, 654, 1091, 1570, 3440, 3796
BstSFI	CTRYAG	2 cut(s)	2379, 2884
BstSLI	GKGCMC	2 cut(s)	116, 241
BstUI	CGCG	2 cut(s)	402, 3088
BstV2I	GAAGAC	1 cut(s)	3116
BstX2I	RGATCY	3 cut(s)	587, 2314, 3047
BstXI	CCANNNNNNTGG	1 cut(s)	1360
BstYI	RGATCY	3 cut(s)	587, 2314, 3047
BsuI	GTATCC	2 cut(s)	895, 2591
BsuRI	GGCC	3 cut(s)	1096, 2283, 3440
BtgI	CCRYGG	2 cut(s)	1346, 2302
BtrI	CACGTC	1 cut(s)	2760
BtsI	GCAGTG	1 cut(s)	2351
BveI	ACCTGC	1 cut(s)	2127
Cac8I	GCNNGC	4 cut(s)	116, 261, 1980, 2328
CaiI	CAGNNNCTG	2 cut(s)	875, 1946
CfoI	GCGC	4 cut(s)	404, 1561, 2664, 3088
Cfr13I	GGNCC	2 cut(s)	1350, 3007
CseI	GACGC	2 cut(s)	67, 3643
Csp6I	GTAC	3 cut(s)	715, 2633, 3445
CspCI	CAANNNNNGTGG	2 cut(s)	807, 842
CviQI	GTAC	3 cut(s)	715, 2633, 3445
DdeI	CTNAG	8 cut(s)	128, 950, 1314, 1661, 1700, 2160, 2322, 3228
DpnI	GATC	9 cut(s)	573, 589, 1140, 1617, 2316, 3049, 3453, 3567, 3613
DpnII	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
DraI	TTTAAA	3 cut(s)	67, 1800, 2049
DrdI	GACNNNNNNGTC	1 cut(s)	534
DseDI	GACNNNNNNGTC	1 cut(s)	534
EaeI	YGGCCR	2 cut(s)	2281, 3438
Eam1104I	CTCTTC	4 cut(s)	171, 321, 1228, 3766
EarI	CTCTTC	4 cut(s)	171, 321, 1228, 3766
EciI	GGCGGA	1 cut(s)	3783
Ecl136II	GAGCTC	1 cut(s)	132
Eco130I	CCWWGG	4 cut(s)	619, 1346, 2302, 2964
Eco147I	AGGCCT	1 cut(s)	1096
Eco24I	GRGCYC	4 cut(s)	134, 2894, 3212, 3628
Eco31I	GGTCTC	1 cut(s)	3647
Eco32I	GATATC	1 cut(s)	1025
Eco47I	GGWCC	2 cut(s)	1350, 3007
Eco53kI	GAGCTC	1 cut(s)	132
Eco57I	CTGAAG	2 cut(s)	260, 3580
Eco88I	CYCGRG	2 cut(s)	469, 3407
EcoICRI	GAGCTC	1 cut(s)	132
EcoO109I	RGGNCCY	1 cut(s)	3007
EcoRI	GAATTC	1 cut(s)	3686
EcoRII	CCWGG	6 cut(s)	304, 443, 654, 1091, 3440, 3796
EcoRV	GATATC	1 cut(s)	1025
EcoT14I	CCWWGG	4 cut(s)	619, 1346, 2302, 2964
EcoT22I	ATGCAT	1 cut(s)	1629
EcoT38I	GRGCYC	4 cut(s)	134, 2894, 3212, 3628
ErhI	CCWWGG	4 cut(s)	619, 1346, 2302, 2964
FalI	AAGNNNNNCTT	4 cut(s)	3115, 3147, 3476, 3508
FauI	CCCGC	1 cut(s)	123
FauNDI	CATATG	3 cut(s)	18, 408, 2553
FblI	GTMKAC	1 cut(s)	2883
FriOI	GRGCYC	4 cut(s)	134, 2894, 3212, 3628
FspBI	CTAG	7 cut(s)	320, 810, 1821, 1956, 2876, 2960, 3387
FspI	TGCGCA	1 cut(s)	2663
GlaI	GCGC	4 cut(s)	403, 1560, 2663, 3087
GsuI	CTGGAG	4 cut(s)	1203, 1382, 1491, 1908
HaeIII	GGCC	3 cut(s)	1096, 2283, 3440
HapII	CCGG	6 cut(s)	192, 821, 1402, 1571, 2011, 3740
HgaI	GACGC	2 cut(s)	67, 3643
HhaI	GCGC	4 cut(s)	404, 1561, 2664, 3088
Hin1I	GRCGYC	1 cut(s)	59
Hin6I	GCGC	4 cut(s)	402, 1559, 2662, 3086
HinP1I	GCGC	4 cut(s)	402, 1559, 2662, 3086
HincII	GTYRAC	6 cut(s)	559, 697, 1297, 1996, 2844, 3792
HindII	GTYRAC	6 cut(s)	559, 697, 1297, 1996, 2844, 3792
HindIII	AAGCTT	3 cut(s)	971, 2973, 3144
HinfI	GANTC	9 cut(s)	22, 397, 629, 1006, 1154, 1178, 1852, 2057, 3126
HpaI	GTTAAC	3 cut(s)	1297, 1996, 2844
HpaII	CCGG	6 cut(s)	192, 821, 1402, 1571, 2011, 3740
HphI	GGTGA	4 cut(s)	1738, 2848, 2981, 3332
Hpy166II	GTNNAC	9 cut(s)	239, 559, 697, 1297, 1996, 2844, 2884, 3092, 3792
Hpy8I	GTNNAC	9 cut(s)	239, 559, 697, 1297, 1996, 2844, 2884, 3092, 3792
Hpy99I	CGWCG	3 cut(s)	351, 665, 2703
HpyAV	CCTTC	9 cut(s)	892, 1301, 2450, 2791, 3020, 3141, 3565, 3667, 3754
HpyCH4IV	ACGT	3 cut(s)	2367, 2759, 2989
HpyF3I	CTNAG	8 cut(s)	128, 950, 1314, 1661, 1700, 2160, 2322, 3228
HpySE526I	ACGT	3 cut(s)	2367, 2759, 2989
Hsp92I	GRCGYC	1 cut(s)	59
HspAI	GCGC	4 cut(s)	402, 1559, 2662, 3086
Kpn2I	TCCGGA	1 cut(s)	3739
KspAI	GTTAAC	3 cut(s)	1297, 1996, 2844
Kzo9I	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
LmnI	GCTCC	5 cut(s)	772, 1557, 2897, 2945, 3670
LweI	GCATC	7 cut(s)	20, 499, 793, 1085, 1762, 2932, 3192
MaeI	CTAG	7 cut(s)	320, 810, 1821, 1956, 2876, 2960, 3387
MaeII	ACGT	3 cut(s)	2367, 2759, 2989
MalI	GATC	9 cut(s)	573, 589, 1140, 1617, 2316, 3049, 3453, 3567, 3613
MboI	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
MfeI	CAATTG	2 cut(s)	1811, 3468
MflI	RGATCY	3 cut(s)	587, 2314, 3047
MhlI	GDGCHC	6 cut(s)	116, 134, 241, 2894, 3212, 3628
MlsI	TGGCCA	2 cut(s)	2283, 3440
MluNI	TGGCCA	2 cut(s)	2283, 3440
MlyI	GAGTC	5 cut(s)	31, 406, 623, 1187, 1861
MmeI	TCCRAC	3 cut(s)	60, 337, 1770
Mox20I	TGGCCA	2 cut(s)	2283, 3440
Mph1103I	ATGCAT	1 cut(s)	1629
MroI	TCCGGA	1 cut(s)	3739
MscI	TGGCCA	2 cut(s)	2283, 3440
MslI	CAYNNNNRTG	2 cut(s)	90, 2208
Msp20I	TGGCCA	2 cut(s)	2283, 3440
MspA1I	CMGCKG	2 cut(s)	1124, 3516
MspI	CCGG	6 cut(s)	192, 821, 1402, 1571, 2011, 3740
MspR9I	CCNGG	7 cut(s)	306, 445, 656, 1093, 1572, 3442, 3798
MunI	CAATTG	2 cut(s)	1811, 3468
Mva1269I	GAATGC	3 cut(s)	1127, 2500, 3387
MvaI	CCWGG	6 cut(s)	306, 445, 656, 1093, 3442, 3798
MvnI	CGCG	2 cut(s)	402, 3088
NciI	CCSGG	1 cut(s)	1572
NcoI	CCATGG	2 cut(s)	1346, 2302
NdeI	CATATG	3 cut(s)	18, 408, 2553
NdeII	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
NlaIV	GGNNCC	6 cut(s)	190, 1269, 1519, 2316, 2893, 3672
NmuCI	GTSAC	5 cut(s)	1062, 1602, 1744, 2531, 2803
NsbI	TGCGCA	1 cut(s)	2663
NsiI	ATGCAT	1 cut(s)	1629
NspI	RCATGY	3 cut(s)	89, 263, 2751
NspV	TTCGAA	1 cut(s)	3295
PaeI	GCATGC	1 cut(s)	263
PaeR7I	CTCGAG	1 cut(s)	3407
PceI	AGGCCT	1 cut(s)	1096
PciI	ACATGT	1 cut(s)	85
PcsI	WCGNNNNNNNCGW	2 cut(s)	476, 2986
PctI	GAATGC	3 cut(s)	1127, 2500, 3387
PfeI	GAWTC	4 cut(s)	1006, 1154, 2057, 3126
PflMI	CCANNNNNTGG	2 cut(s)	706, 3221
PfoI	TCCNGGA	2 cut(s)	1570, 3796
PleI	GAGTC	5 cut(s)	30, 405, 623, 1186, 1860
PpsI	GAGTC	5 cut(s)	30, 405, 623, 1186, 1860
PpuMI	RGGWCCY	1 cut(s)	3007
PscI	ACATGT	1 cut(s)	85
PshAI	GACNNNNGTC	1 cut(s)	951
PshBI	ATTAAT	2 cut(s)	429, 1425
Psp124BI	GAGCTC	1 cut(s)	134
Psp1406I	AACGTT	1 cut(s)	2367
Psp5II	RGGWCCY	1 cut(s)	3007
Psp6I	CCWGG	6 cut(s)	304, 443, 654, 1091, 3440, 3796
PspGI	CCWGG	6 cut(s)	304, 443, 654, 1091, 3440, 3796
PspN4I	GGNNCC	6 cut(s)	190, 1269, 1519, 2316, 2893, 3672
PspPI	GGNCC	2 cut(s)	1350, 3007
PspPPI	RGGWCCY	1 cut(s)	3007
PstNI	CAGNNNCTG	2 cut(s)	875, 1946
PsuI	RGATCY	3 cut(s)	587, 2314, 3047
PvuII	CAGCTG	1 cut(s)	1124
RsaI	GTAC	3 cut(s)	716, 2634, 3446
RsaNI	GTAC	3 cut(s)	715, 2633, 3445
RseI	CAYNNNNRTG	2 cut(s)	90, 2208
SacI	GAGCTC	1 cut(s)	134
Sau3AI	GATC	9 cut(s)	571, 587, 1138, 1615, 2314, 3047, 3451, 3565, 3611
Sau96I	GGNCC	2 cut(s)	1350, 3007
ScaI	AGTACT	1 cut(s)	2634
SchI	GAGTC	5 cut(s)	31, 406, 623, 1187, 1861
ScrFI	CCNGG	7 cut(s)	306, 445, 656, 1093, 1572, 3442, 3798
SduI	GDGCHC	6 cut(s)	116, 134, 241, 2894, 3212, 3628
SfaNI	GCATC	7 cut(s)	20, 499, 793, 1085, 1762, 2932, 3192
SfcI	CTRYAG	2 cut(s)	2379, 2884
Sfr274I	CTCGAG	1 cut(s)	3407
SfuI	TTCGAA	1 cut(s)	3295
SinI	GGWCC	2 cut(s)	1350, 3007
SlaI	CTCGAG	1 cut(s)	3407
SmiI	ATTTAAAT	1 cut(s)	1800
SmiMI	CAYNNNNRTG	2 cut(s)	90, 2208
SmlI	CTYRAG	4 cut(s)	1016, 1116, 2236, 3407
SmoI	CTYRAG	4 cut(s)	1016, 1116, 2236, 3407
SpeI	ACTAGT	1 cut(s)	809
SphI	GCATGC	1 cut(s)	263
SseBI	AGGCCT	1 cut(s)	1096
SsiI	CCGC	7 cut(s)	116, 338, 506, 1953, 2419, 3516, 3768
SspI	AATATT	3 cut(s)	688, 790, 3273
SspMI	CTAG	7 cut(s)	320, 810, 1821, 1956, 2876, 2960, 3387
SstI	GAGCTC	1 cut(s)	134
StuI	AGGCCT	1 cut(s)	1096
StyD4I	CCNGG	7 cut(s)	304, 443, 654, 1091, 1570, 3440, 3796
StyI	CCWWGG	4 cut(s)	619, 1346, 2302, 2964
SwaI	ATTTAAAT	1 cut(s)	1800
TaiI	ACGT	3 cut(s)	2370, 2762, 2992
TaqI	TCGA	5 cut(s)	2479, 2542, 3295, 3408, 3614
TatI	WGTACW	1 cut(s)	2632
TfiI	GAWTC	4 cut(s)	1006, 1154, 2057, 3126
TseFI	GTSAC	5 cut(s)	1062, 1602, 1744, 2531, 2803
Tsp45I	GTSAC	5 cut(s)	1062, 1602, 1744, 2531, 2803
TspGWI	ACGGA	4 cut(s)	468, 474, 3462, 3650
Van91I	CCANNNNNTGG	2 cut(s)	706, 3221
VneI	GTGCAC	1 cut(s)	237
VpaK11BI	GGWCC	2 cut(s)	1350, 3007
VspI	ATTAAT	2 cut(s)	429, 1425
XapI	RAATTY	5 cut(s)	136, 1795, 2032, 3576, 3686
XbaI	TCTAGA	1 cut(s)	2875
XceI	RCATGY	3 cut(s)	89, 263, 2751
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2471
XhoI	CTCGAG	1 cut(s)	3407
XmiI	GTMKAC	1 cut(s)	2883
XspI	CTAG	7 cut(s)	320, 810, 1821, 1956, 2876, 2960, 3387
ZrmI	AGTACT	1 cut(s)	2634
Zsp2I	ATGCAT	1 cut(s)	1629

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.