Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0000962

Physical Location & Seq

Genomic Coordinates Forward (+)

5320727 .. 5330139

Gene Structure 9413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1057400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3678 bp

ATGAATAGGGCACCTCATATGAGGCAAGATCGTCCCAATCTGGGTCATGATTTCTGTGCCCAGACGCCTGTTAAAGCTAATTTGTTGGAACATGTCAATGCATTTGATAATTTCTCGTGCCCGCATAGTGTTCTGAGTTCAAATTTTATTTTCTCCTTACCAACACAAAAACCTCTTGCCGAAGAGGCAATGGTTTCCGGCTCAATGGCTTGTCCAATTCAGAATGCTGAAAGATTACAGAGTGCACAAGTTCAAAAGGCTTGGCATGTCCTTTCCAATCTACAGATTTCTTGTAGGAACTACCCAAAACCTGGAAAAACTCTTCTAGTTAAGGATGTTAGTGCCGAGTATCGTCCTGATGTTGGAAGAACTGCTTTGCCAAGCTCATGCAACATTGATAAGAGTCGCGCACATATGCAAGCGCATCAATACAATAATGGAACTAATACCAGGAACAGTGAAGCAGCAACTTGTTCGGGCAATCCGCTTCCATCAAGTAATTTTAATGTTGCAGATGCAGGAAAAAATCTTGGACGGCAAAGTCAGGTCAGAGCATCAATGGTCAACAGTTCTGATGCTCGGGTGTTGGATGGATCACTTAGCAACAATAGTGTTCATACCAGCCAAGGGAAAGACTCTGCAGAAGTTTTTGTTAATGACCTGGACGACGATGATGATGACATACTTGAGAATATTGATGTTGACCAGATAGTGGAACAGTACCAATCAAGTTGTACACCTCAACCATCAATTTCCAAGCTTCCACCTTTTACTCCCTTTATAGATAAAGATAACAAACAAGAGGAGACTAGTTTGCCACCAGAATTGTGTTCTAATTGCAGTCATGGGTTTAAGATAGGACTTTGCCCAGAAGCTGGAAGTCATTTGCAGGAAATGAAGGATACCCTAATTATCATATCAAATGAACTGCTTGATGATGTTAATGACCTCAGCCCAGAACAAATAAATAAGCTTCGCCAATATAGGTTACAGCTTAATAAGCAGATTCAGCAGCTTGAGAAATATCTTTGTAACAACTCATTGGATGAAGAAAGACGAAAGTCACATTTTTCTGCATCAACAGCAACTCCCAGGCCTTTCCAATGTGAAACACCTCAAGCAGCTGCATTCAGGACTGATCCCATGAGATTTGATTCACAGGTTCATCCACATAACGAGTCTGGAGGATATGAAAGATGTAATTCATCATCAGTTTCATTCTCTTCTGTTGATAGGCTTGGTTTCTCATCTTGTCCTGTTGAGAGGGAACCATGCATTTCAAAGTTTGTTGAAGTTAACTACATTGAAGGTTCCACGGACAAAAAATGGAGTAGTGATAATTTTCCGTGGACCAAAAAGCTGGAGGATGGTAACAAGAAAGTGTTTGGAAATCACTCTTTCCGTCTCAACCAAAGAGAGGTCATTAATGCTACGATGAGTGGCTATGATGTTTTTGTTTTAATGCCAACTGGAGGGGGTAAGAGCTTGACATATCAGCTCCCTGCGCTAATTTGTCAAGGAATAACATTGGTTATTTCTCCCCTTGTGTCACTCATTCAAGATCAAATAATGCATTTATTGCAGGCAAACATTCCCGCTGCTTACCTAAGTGCCAATATGGAGTGGACTGAACAACAGGAAATACTTAGAGAACTAAATTATGAATATTGTAAATACAAGCTGTTATATGTCACCCCAGAGAAAGTTGCTAAAAGTGATGCTCTTTTACGGCAGTTGGAGAATTTAAATGCTCGTCAATTGCTTGCTAGGATTGTTGTTGATGAAGCTCATTGTGTGAGTCAGTGGGGGCATGATTTCAGACCAGATTATCAGGCTCTTGGTATCTTGAAACAGAAGTTCCCAAATACTCCAGTTTTAGCTTTAACAGCTACTGCAACCGCTAGTGTAAAAGAAGATGTTGTGCAAGCTCTTGGTCTTGTTAACTGCATTGTTTTCCGGCAAAGTTTTAATCGCCCAAATTTACGGTATTCTGTTATTCCCAAGACTAAGAAGTGTCTTGATGACATTGACAAGTTCATTAAAGAAAATCATTATGATGAAAGTGGAATAATTTATTGTCTCTCAAGAATGGACTGTGAAAAAGTTGCTGAAAGATTACAGGAATGTGGCCATAAAGCAGCATTTTACCATGGTAGTATGGATCCTGCTCAGCGTGCCTTTGTCCAGAAGCAGTGGAGTAAAGATGAAATCAATGTTATATGTGCTACAGTGGCATTTGGAATGGGTATCAACAAGCCAGATGTCCGCTTTGTAATCCATCACTCTCTGCCGAAGTCTATTGAAGGCTACCATCAGGAGTGTGGTCGAGCTGGTAGAGATGGTCAGCGTTCATCTTGTGTGTTGTATTACAGTTACAGTGACTATATTCGAGTGAAGCATATGATTAGTCAAGGAGTTATAGAGCAAAGCCCACTGGCATCTGGATACAATCGTATCATTACTGCTAATTCTGGGAGAGTACTGGAAACAAACACCGAAAATCTTTTGCGCATGGTCAGTTATTGTGAAAATGATGTTGATTGCCGACGACTTCTACAGCTCATTCATTTTGGAGAAAACTTTGATTCTGCAACCTGCAAAAAGACGTGTGATAATTGTTTGAAGATTAGAACTTTCAGTGAGAAAGATGTCACTGAGATTGCAAAGCAACTGGTTGAACTAGTGAAGTTAACAGGGCAGCAGTTTTCATCATCTCATATTCTAGAAGTCTACAGGGGCTCCTTAAACCAATTTGTCAAGAAACACAGGCATCAGACTGTGAGCCTGCATGGAGCAGGAAAACATCTAGCCAAGGGTGAAGGTTCTCGTGTATTACGTCATCTTGTTACTGAGGACCTTCTTGTTGAGGAAGTCAAGAAAAGTGATGTTTATGGATCTGTATCGTCAATACTTAAGGCTAATGAACTGAAAGTGCGCGAACTTTTCTCTGGCAGGAAGACAATTCTAGTAAGATTCCCTTCCACCTTTAAAGCTTCCAAACAAATAAACTCCGAAGCGATTTCAGCTCAATGCTCACTGATGTCTGGGATGCAGAGCCATCCACATACTGGCACTGAACAACCTCAATCAAAAGGAGATTTGGTTCTGTATACGAAAACATTTTCTGCTTTGAAAATGCTTCGAACTAATCTCATTAGAGAAGCTGGTGATGGGGTCATGGCACACCACATATTTCAGAATACCACGATTAAAAACATAAGCATCAGAATGCCTAGAACGAAGGATGAACTACTTGAGATAAATGGCATTACCCAAGTTAAGGCGGCCAGGTATGGAGATCGAATACTTGAAACAATTGAAGCTGCCATAAAGGAATACTTCAAAACAGATAAAAACAGCAGCGGTAGCAGTGACAGCAATGATATAAAGAGGAAAAGAGATGGTAACAGAGATCGGAAGGAAAATGTTGAAGATGACGACTTCAAAAGCACAGATAGATTGAAGAAAATGGCACTAAAGAAGCAGAGCAAAACCATTGAGGCCTATAGTTATGTGGAGGCAGATTACAGCGTATTCATAGATGATGAATTAGACTTATCTGGTTATGACTTTGAAGCTAATGTTCCGGATATGAAAACCAATCAGAACGGTGGCGGAAGAGTGCTGCCACAGTGGTCAACACCAGGAAATGGGCAGACATCGGTAAAATTTTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005911 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009266 GO:0009378 GO:0009409 GO:0009506 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030054 GO:0032392 GO:0032508 GO:0032870 GO:0033554 GO:0033993 GO:0034641 GO:0042221 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0055044 GO:0070035 GO:0070417 GO:0070887 GO:0071103 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097305 GO:0097306 GO:0140097 GO:1901360 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko03018 ko03440 ko03460 map03018 map03440 map03460

Pfam Domains

Protein Families

DEAD HRDC Helicase_C RQC RecQ_Zn_bind

Protein Analysis

1226

Amino Acids

137.68

Weight (kDa)

7.05

Isoelectric Point (pI)

42.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	466 - 632	7.5e-06	Type III restriction enzyme, res subunit
DEAD	PF00270	470 - 637	6.3e-25	DEAD/DEAH box helicase
Helicase_C	PF00271	674 - 781	1.7e-19	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	792 - 874	7e-16	RecQ zinc-binding
RQC	PF09382	880 - 996	3.4e-20	RQC domain
HRDC	PF00570	1041 - 1106	2.4e-12	HRDC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000962)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10930
fragaria_vesca	FvH4_5g28920 FvH4_5g28920
malus_domestica	MD02G1262900.v1.1 MD07G1056800.v1.1 MD07G1057400.v1.1
prunus_persica	Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1 Prupe.4G271400_v2.0.a1
pyrus_communis	pycom02g22480 pycom02g22490 pycom02g22530 pycom07g04380
rosa_chinensis	RchiOBHm_Chr2g0101731 RchiOBHm_Chr2g0146651 RchiOBHm_Chr3g0471841 RchiOBHm_Chr4g0402091 RchiOBHm_Chr5g0019321 RchiOBHm_Chr7g0221231
rosa_laevigata	RLG00000002168 RLG00000008436
rosa_multiflora	Rmu_co8338979.1_g000001 Rmu_co8389917.1_g000001 Rmu_sc0000212.1_g000013 Rmu_sc0000324.1_g000016 Rmu_sc0000518.1_g000001 Rmu_sc0000808.1_g000043 Rmu_sc0002206.1_g000018 Rmu_sc0002551.1_g000024 Rmu_sc0004296.1_g000023 Rmu_sc0009806.1_g000017 Rmu_sc0032411.1_g000001 Rmu_ssc0000291.1_g000025
rosa_roxburghii	Rroxscaffold_176G00431210 Rroxscaffold_1G00026280 Rroxscaffold_3G00238540 Rroxscaffold_4G00313160 Rroxscaffold_4G00330040 Rroxscaffold_5G00342230 Rroxscaffold_5G00352050
rosa_rugosa	Rorug07G0201600 Rorug07G0253300
rosa_samantha	Rh1BG131100 Rh1CG101400 Rh2BG215600 Rh2CG271000 Rh4DG065200 Rh4DG134600 Rh5AG275200 Rh5AG434000 Rh7AG340500 Rh7BG172400 Rh7BG331600 Rh7BG331700 Rh7CG358400 Rh7DG335700 Rh7DG335800 Rh7DG339200 Rh7DG339300
rosa_wichuraiana	Rw6G023870 Rw7G028980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	540
Acc16I	TGCGCA	1 cut(s)	2510
Acc36I	ACCTGC	1 cut(s)	2603
AccB1I	GGYRCC	1 cut(s)	10
AccB7I	CCANNNNNTGG	5 cut(s)	311, 712, 875, 3068, 3650
AccI	GTMKAC	2 cut(s)	2730, 3110
AccII	CGCG	2 cut(s)	408, 2937
AccIII	TCCGGA	1 cut(s)	3586
AciI	CCGC	8 cut(s)	122, 485, 1596, 1899, 2266, 3284, 3363, 3615
AclWI	GGATC	5 cut(s)	601, 1133, 2156, 2169, 2902
AcoI	YGGCCR	2 cut(s)	2128, 3285
AcsI	RAATTY	4 cut(s)	142, 1741, 1978, 3668
AcyI	GRCGYC	1 cut(s)	65
AfaI	GTAC	3 cut(s)	722, 736, 2481
AflII	CTTAAG	1 cut(s)	2912
AflIII	ACRYGT	2 cut(s)	91, 2606
AhlI	ACTAGT	2 cut(s)	809, 2680
AjiI	CACGTC	1 cut(s)	2607
AjnI	CCWGG	6 cut(s)	310, 449, 660, 1091, 3287, 3643
AloI	GAACNNNNNNTCC	2 cut(s)	3087, 3119
Alw21I	GWGCWC	1 cut(s)	247
Alw26I	GTCTC	3 cut(s)	800, 1409, 2084
Alw44I	GTGCAC	1 cut(s)	243
AlwI	GGATC	5 cut(s)	601, 1133, 2156, 2169, 2902
AlwNI	CAGNNNCTG	2 cut(s)	875, 1892
Ama87I	CYCGRG	1 cut(s)	579
Aor13HI	TCCGGA	1 cut(s)	3586
AoxI	GGCC	4 cut(s)	1094, 2128, 3285, 3501
ApaLI	GTGCAC	1 cut(s)	243
ApoI	RAATTY	4 cut(s)	142, 1741, 1978, 3668
AseI	ATTAAT	1 cut(s)	1425
AspLEI	GCGC	5 cut(s)	410, 424, 1507, 2511, 2937
AspS9I	GGNCC	2 cut(s)	1350, 2854
AsuHPI	GGTGA	3 cut(s)	1684, 2828, 3179
AsuII	TTCGAA	1 cut(s)	3142
AvaI	CYCGRG	1 cut(s)	579
AvaII	GGWCC	2 cut(s)	1350, 2854
BaeGI	GKGCMC	4 cut(s)	13, 61, 122, 247
BaeI	ACNNNNGTAYC	4 cut(s)	2437, 2437, 2470, 2470
BalI	TGGCCA	1 cut(s)	2130
BamHI	GGATCC	1 cut(s)	2161
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	1 cut(s)	2741
BauI	CACGAG	2 cut(s)	115, 2826
BbsI	GAAGAC	1 cut(s)	2963
Bbv12I	GWGCWC	1 cut(s)	247
BbvCI	CCTCAGC	1 cut(s)	950
BceAI	ACGGC	2 cut(s)	551, 1745
BcgI	CGANNNNNNTGC	2 cut(s)	456, 490
BciT130I	CCWGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
BciVI	GTATCC	2 cut(s)	895, 2438
BcoDI	GTCTC	3 cut(s)	800, 1409, 2084
BcuI	ACTAGT	2 cut(s)	809, 2680
BfaI	CTAG	9 cut(s)	326, 810, 1767, 1902, 2681, 2723, 2807, 2966, 3234
BfmI	CTRYAG	6 cut(s)	281, 639, 2226, 2555, 2731, 3505
BfrI	CTTAAG	1 cut(s)	2912
BfuAI	ACCTGC	1 cut(s)	2603
BfuI	GTATCC	2 cut(s)	895, 2438
BglI	GCCNNNNNGGC	1 cut(s)	185
BlpI	GCTNAGC	1 cut(s)	2169
BmcAI	AGTACT	1 cut(s)	2481
Bme1390I	CCNGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
Bme18I	GGWCC	2 cut(s)	1350, 2854
BmeT110I	CYCGRG	1 cut(s)	579
BmgBI	CACGTC	1 cut(s)	2607
BmgT120I	GGNCC	2 cut(s)	1350, 2854
BmiI	GGNNCC	5 cut(s)	12, 1269, 1312, 2163, 2740
BmrFI	CCNGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
BoxI	GACNNNNGTC	1 cut(s)	1060
BpiI	GAAGAC	1 cut(s)	2963
BpmI	CTGGAG	4 cut(s)	1203, 1382, 1491, 1854
Bpu10I	CCTNAGC	1 cut(s)	950
Bpu1102I	GCTNAGC	1 cut(s)	2169
Bpu14I	TTCGAA	1 cut(s)	3142
BpuEI	CTTGAG	5 cut(s)	707, 1037, 1101, 2068, 3275
BsaBI	GATNNNNATC	1 cut(s)	2899
BsaHI	GRCGYC	1 cut(s)	65
BsaJI	CCNNGG	6 cut(s)	625, 1091, 1314, 1346, 2149, 2811
BsaWI	WCCGGW	1 cut(s)	3586
BsaXI	ACNNNNNCTCC	6 cut(s)	1072, 1102, 2723, 2753, 3087, 3117
Bse1I	ACTGG	6 cut(s)	1474, 1871, 2439, 2487, 2676, 3073
Bse3DI	GCAATG	2 cut(s)	195, 3385
Bse8I	GATNNNNATC	1 cut(s)	2899
BseAI	TCCGGA	1 cut(s)	3586
BseBI	CCWGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
BseDI	CCNNGG	6 cut(s)	625, 1091, 1314, 1346, 2149, 2811
BseGI	GGATG	8 cut(s)	340, 595, 1051, 1165, 1372, 3054, 3058, 3250
BseJI	GATNNNNATC	1 cut(s)	2899
BseMI	GCAATG	2 cut(s)	195, 3385
BseMII	CTCAG	5 cut(s)	125, 964, 2183, 2646, 2841
BseNI	ACTGG	6 cut(s)	1474, 1871, 2439, 2487, 2676, 3073
BseRI	GAGGAG	1 cut(s)	818
BseSI	GKGCMC	4 cut(s)	13, 61, 122, 247
Bsh1236I	CGCG	2 cut(s)	408, 2937
BshFI	GGCC	4 cut(s)	1096, 2130, 3287, 3503
BshNI	GGYRCC	1 cut(s)	10
BsiHKAI	GWGCWC	1 cut(s)	247
BsiHKCI	CYCGRG	1 cut(s)	579
BsiSI	CCGG	3 cut(s)	198, 1957, 3587
BslFI	GGGAC	1 cut(s)	18
BsmAI	GTCTC	3 cut(s)	800, 1409, 2084
BsmBI	CGTCTC	1 cut(s)	1409
BsmFI	GGGAC	1 cut(s)	18
BsmI	GAATGC	3 cut(s)	229, 1127, 3234
BsnI	GGCC	4 cut(s)	1096, 2130, 3287, 3503
BsoBI	CYCGRG	1 cut(s)	579
Bsp119I	TTCGAA	1 cut(s)	3142
Bsp1286I	GDGCHC	5 cut(s)	13, 61, 122, 247, 2741
Bsp13I	TCCGGA	1 cut(s)	3586
Bsp1407I	TGTACA	1 cut(s)	734
Bsp143I	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
Bsp1720I	GCTNAGC	1 cut(s)	2169
Bsp19I	CCATGG	1 cut(s)	2149
BspACI	CCGC	8 cut(s)	122, 485, 1596, 1899, 2266, 3284, 3363, 3615
BspANI	GGCC	4 cut(s)	1096, 2130, 3287, 3503
BspCNI	CTCAG	5 cut(s)	126, 963, 2182, 2647, 2842
BspEI	TCCGGA	1 cut(s)	3586
BspFNI	CGCG	2 cut(s)	408, 2937
BspHI	TCATGA	1 cut(s)	46
BspLI	GGNNCC	5 cut(s)	12, 1269, 1312, 2163, 2740
BspMAI	CTGCAG	1 cut(s)	643
BspMI	ACCTGC	1 cut(s)	2603
BspPI	GGATC	5 cut(s)	601, 1133, 2156, 2169, 2902
BspT104I	TTCGAA	1 cut(s)	3142
BspT107I	GGYRCC	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	2912
BsrDI	GCAATG	2 cut(s)	195, 3385
BsrGI	TGTACA	1 cut(s)	734
BsrI	ACTGG	6 cut(s)	1474, 1871, 2439, 2487, 2676, 3073
BssECI	CCNNGG	6 cut(s)	625, 1091, 1314, 1346, 2149, 2811
BssMI	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
BssNAI	GTATAC	1 cut(s)	3111
BssNI	GRCGYC	1 cut(s)	65
BssSI	CACGAG	2 cut(s)	115, 2826
BssT1I	CCWWGG	3 cut(s)	625, 2149, 2811
Bst1107I	GTATAC	1 cut(s)	3111
Bst2BI	CACGAG	2 cut(s)	115, 2826
Bst2UI	CCWGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
Bst6I	CTCTTC	4 cut(s)	177, 327, 1228, 3613
BstACI	GRCGYC	1 cut(s)	65
BstAFI	CTTAAG	1 cut(s)	2912
BstAPI	GCANNNNNTGC	1 cut(s)	1579
BstAUI	TGTACA	1 cut(s)	734
BstBI	TTCGAA	1 cut(s)	3142
BstC8I	GCNNGC	7 cut(s)	122, 420, 1584, 1764, 1926, 2175, 2786
BstDEI	CTNAG	9 cut(s)	134, 599, 950, 1607, 1646, 2007, 2169, 2655, 2850
BstDSI	CCRYGG	3 cut(s)	1314, 1346, 2149
BstF5I	GGATG	8 cut(s)	340, 595, 1051, 1165, 1372, 3054, 3058, 3250
BstFNI	CGCG	2 cut(s)	408, 2937
BstHHI	GCGC	5 cut(s)	410, 424, 1507, 2511, 2937
BstKTI	GATC	8 cut(s)	31, 596, 1141, 1564, 2164, 2897, 3301, 3415
BstMAI	GTCTC	3 cut(s)	800, 1409, 2084
BstMBI	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
BstNI	CCWGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
BstNSI	RCATGY	2 cut(s)	95, 269
BstPAI	GACNNNNGTC	1 cut(s)	1060
BstSCI	CCNGG	6 cut(s)	310, 449, 660, 1091, 3287, 3643
BstSFI	CTRYAG	6 cut(s)	281, 639, 2226, 2555, 2731, 3505
BstSLI	GKGCMC	4 cut(s)	13, 61, 122, 247
BstUI	CGCG	2 cut(s)	408, 2937
BstV2I	GAAGAC	1 cut(s)	2963
BstX2I	RGATCY	2 cut(s)	2161, 2894
BstXI	CCANNNNNNTGG	1 cut(s)	1360
BstYI	RGATCY	2 cut(s)	2161, 2894
BstZ17I	GTATAC	1 cut(s)	3111
BsuI	GTATCC	2 cut(s)	895, 2438
BsuRI	GGCC	4 cut(s)	1096, 2130, 3287, 3503
BtgI	CCRYGG	3 cut(s)	1314, 1346, 2149
BtrI	CACGTC	1 cut(s)	2607
BtsCI	GGATG	8 cut(s)	340, 595, 1051, 1165, 1372, 3054, 3058, 3250
BtsI	GCAGTG	2 cut(s)	2198, 3376
BveI	ACCTGC	1 cut(s)	2603
Cac8I	GCNNGC	7 cut(s)	122, 420, 1584, 1764, 1926, 2175, 2786
CaiI	CAGNNNCTG	2 cut(s)	875, 1892
CciI	TCATGA	1 cut(s)	46
CfoI	GCGC	5 cut(s)	410, 424, 1507, 2511, 2937
Cfr13I	GGNCC	2 cut(s)	1350, 2854
CseI	GACGC	1 cut(s)	73
Csp6I	GTAC	3 cut(s)	721, 735, 2480
CspCI	CAANNNNNGTGG	2 cut(s)	807, 842
CviQI	GTAC	3 cut(s)	721, 735, 2480
DdeI	CTNAG	9 cut(s)	134, 599, 950, 1607, 1646, 2007, 2169, 2655, 2850
DpnI	GATC	8 cut(s)	30, 595, 1140, 1563, 2163, 2896, 3300, 3414
DpnII	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
DraI	TTTAAA	2 cut(s)	1746, 2989
DrdI	GACNNNNNNGTC	1 cut(s)	540
DseDI	GACNNNNNNGTC	1 cut(s)	540
EaeI	YGGCCR	2 cut(s)	2128, 3285
Eam1104I	CTCTTC	4 cut(s)	177, 327, 1228, 3613
EarI	CTCTTC	4 cut(s)	177, 327, 1228, 3613
EciI	GGCGGA	1 cut(s)	3630
Eco130I	CCWWGG	3 cut(s)	625, 2149, 2811
Eco147I	AGGCCT	2 cut(s)	1096, 3503
Eco24I	GRGCYC	1 cut(s)	2741
Eco47I	GGWCC	2 cut(s)	1350, 2854
Eco88I	CYCGRG	1 cut(s)	579
EcoO109I	RGGNCCY	1 cut(s)	2854
EcoRII	CCWGG	6 cut(s)	310, 449, 660, 1091, 3287, 3643
EcoT14I	CCWWGG	3 cut(s)	625, 2149, 2811
EcoT22I	ATGCAT	3 cut(s)	103, 1277, 1575
EcoT38I	GRGCYC	1 cut(s)	2741
ErhI	CCWWGG	3 cut(s)	625, 2149, 2811
Esp3I	CGTCTC	1 cut(s)	1409
FalI	AAGNNNNNCTT	6 cut(s)	358, 390, 2962, 2994, 3323, 3355
FaqI	GGGAC	1 cut(s)	18
FauI	CCCGC	2 cut(s)	129, 1603
FauNDI	CATATG	3 cut(s)	18, 414, 2400
FblI	GTMKAC	2 cut(s)	2730, 3110
FokI	GGATG	8 cut(s)	347, 602, 1058, 1152, 1379, 3045, 3061, 3257
FriOI	GRGCYC	1 cut(s)	2741
FspBI	CTAG	9 cut(s)	326, 810, 1767, 1902, 2681, 2723, 2807, 2966, 3234
FspI	TGCGCA	1 cut(s)	2510
GlaI	GCGC	5 cut(s)	409, 423, 1506, 2510, 2936
GsuI	CTGGAG	4 cut(s)	1203, 1382, 1491, 1854
HaeIII	GGCC	4 cut(s)	1096, 2130, 3287, 3503
HapII	CCGG	3 cut(s)	198, 1957, 3587
HgaI	GACGC	1 cut(s)	73
HhaI	GCGC	5 cut(s)	410, 424, 1507, 2511, 2937
Hin1I	GRCGYC	1 cut(s)	65
Hin6I	GCGC	5 cut(s)	408, 422, 1505, 2509, 2935
HinP1I	GCGC	5 cut(s)	408, 422, 1505, 2509, 2935
HincII	GTYRAC	6 cut(s)	565, 703, 1297, 1942, 2691, 3639
HindII	GTYRAC	6 cut(s)	565, 703, 1297, 1942, 2691, 3639
HindIII	AAGCTT	3 cut(s)	758, 971, 2991
HinfI	GANTC	8 cut(s)	403, 635, 1006, 1154, 1178, 1798, 2585, 2973
HpaI	GTTAAC	3 cut(s)	1297, 1942, 2691
HpaII	CCGG	3 cut(s)	198, 1957, 3587
HphI	GGTGA	3 cut(s)	1684, 2828, 3179
Hpy99I	CGWCG	2 cut(s)	671, 2550
HpyAV	CCTTC	8 cut(s)	892, 1301, 2297, 2813, 2867, 2988, 3235, 3412
HpyCH4IV	ACGT	2 cut(s)	2606, 2836
HpyF3I	CTNAG	9 cut(s)	134, 599, 950, 1607, 1646, 2007, 2169, 2655, 2850
HpySE526I	ACGT	2 cut(s)	2606, 2836
Hsp92I	GRCGYC	1 cut(s)	65
HspAI	GCGC	5 cut(s)	408, 422, 1505, 2509, 2935
Kpn2I	TCCGGA	1 cut(s)	3586
KspAI	GTTAAC	3 cut(s)	1297, 1942, 2691
Kzo9I	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
LmnI	GCTCC	3 cut(s)	1503, 2744, 2792
MaeI	CTAG	9 cut(s)	326, 810, 1767, 1902, 2681, 2723, 2807, 2966, 3234
MaeII	ACGT	2 cut(s)	2606, 2836
MalI	GATC	8 cut(s)	30, 595, 1140, 1563, 2163, 2896, 3300, 3414
MboI	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
MfeI	CAATTG	2 cut(s)	1757, 3315
MflI	RGATCY	2 cut(s)	2161, 2894
MhlI	GDGCHC	5 cut(s)	13, 61, 122, 247, 2741
MlsI	TGGCCA	1 cut(s)	2130
MluNI	TGGCCA	1 cut(s)	2130
MlyI	GAGTC	4 cut(s)	412, 629, 1187, 1807
MmeI	TCCRAC	4 cut(s)	66, 343, 567, 1716
Mox20I	TGGCCA	1 cut(s)	2130
Mph1103I	ATGCAT	3 cut(s)	103, 1277, 1575
MroI	TCCGGA	1 cut(s)	3586
MscI	TGGCCA	1 cut(s)	2130
MslI	CAYNNNNRTG	3 cut(s)	96, 2055, 3227
Msp20I	TGGCCA	1 cut(s)	2130
MspA1I	CMGCKG	3 cut(s)	1124, 1598, 3363
MspCI	CTTAAG	1 cut(s)	2912
MspI	CCGG	3 cut(s)	198, 1957, 3587
MspR9I	CCNGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
MunI	CAATTG	2 cut(s)	1757, 3315
Mva1269I	GAATGC	3 cut(s)	229, 1127, 3234
MvaI	CCWGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
MvnI	CGCG	2 cut(s)	408, 2937
NcoI	CCATGG	1 cut(s)	2149
NdeI	CATATG	3 cut(s)	18, 414, 2400
NdeII	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
NlaIV	GGNNCC	5 cut(s)	12, 1269, 1312, 2163, 2740
NmeAIII	GCCGAG	1 cut(s)	370
NmuCI	GTSAC	6 cut(s)	1062, 1548, 1690, 2378, 2650, 3371
NsbI	TGCGCA	1 cut(s)	2510
NsiI	ATGCAT	3 cut(s)	103, 1277, 1575
NspI	RCATGY	2 cut(s)	95, 269
NspV	TTCGAA	1 cut(s)	3142
PagI	TCATGA	1 cut(s)	46
PceI	AGGCCT	2 cut(s)	1096, 3503
PciI	ACATGT	1 cut(s)	91
PcsI	WCGNNNNNNNCGW	1 cut(s)	2833
PctI	GAATGC	3 cut(s)	229, 1127, 3234
PfeI	GAWTC	4 cut(s)	1006, 1154, 2585, 2973
PflMI	CCANNNNNTGG	5 cut(s)	311, 712, 875, 3068, 3650
PleI	GAGTC	4 cut(s)	411, 629, 1186, 1806
PpsI	GAGTC	4 cut(s)	411, 629, 1186, 1806
PpuMI	RGGWCCY	1 cut(s)	2854
PscI	ACATGT	1 cut(s)	91
PshAI	GACNNNNGTC	1 cut(s)	1060
PshBI	ATTAAT	1 cut(s)	1425
Psp5II	RGGWCCY	1 cut(s)	2854
Psp6I	CCWGG	6 cut(s)	310, 449, 660, 1091, 3287, 3643
PspGI	CCWGG	6 cut(s)	310, 449, 660, 1091, 3287, 3643
PspN4I	GGNNCC	5 cut(s)	12, 1269, 1312, 2163, 2740
PspPI	GGNCC	2 cut(s)	1350, 2854
PspPPI	RGGWCCY	1 cut(s)	2854
PstI	CTGCAG	1 cut(s)	643
PstNI	CAGNNNCTG	2 cut(s)	875, 1892
PsuI	RGATCY	2 cut(s)	2161, 2894
PvuII	CAGCTG	1 cut(s)	1124
RsaI	GTAC	3 cut(s)	722, 736, 2481
RsaNI	GTAC	3 cut(s)	721, 735, 2480
RseI	CAYNNNNRTG	3 cut(s)	96, 2055, 3227
Sau3AI	GATC	8 cut(s)	28, 593, 1138, 1561, 2161, 2894, 3298, 3412
Sau96I	GGNCC	2 cut(s)	1350, 2854
ScaI	AGTACT	1 cut(s)	2481
SchI	GAGTC	4 cut(s)	412, 629, 1187, 1807
ScrFI	CCNGG	6 cut(s)	312, 451, 662, 1093, 3289, 3645
SduI	GDGCHC	5 cut(s)	13, 61, 122, 247, 2741
SfcI	CTRYAG	6 cut(s)	281, 639, 2226, 2555, 2731, 3505
SfuI	TTCGAA	1 cut(s)	3142
SinI	GGWCC	2 cut(s)	1350, 2854
SmiI	ATTTAAAT	1 cut(s)	1746
SmiMI	CAYNNNNRTG	3 cut(s)	96, 2055, 3227
SmlI	CTYRAG	6 cut(s)	686, 1016, 1116, 2083, 2912, 3254
SmoI	CTYRAG	6 cut(s)	686, 1016, 1116, 2083, 2912, 3254
SpeI	ACTAGT	2 cut(s)	809, 2680
SseBI	AGGCCT	2 cut(s)	1096, 3503
SsiI	CCGC	8 cut(s)	122, 485, 1596, 1899, 2266, 3284, 3363, 3615
SspI	AATATT	2 cut(s)	694, 1667
SspMI	CTAG	9 cut(s)	326, 810, 1767, 1902, 2681, 2723, 2807, 2966, 3234
StuI	AGGCCT	2 cut(s)	1096, 3503
StyD4I	CCNGG	6 cut(s)	310, 449, 660, 1091, 3287, 3643
StyI	CCWWGG	3 cut(s)	625, 2149, 2811
SwaI	ATTTAAAT	1 cut(s)	1746
TaiI	ACGT	2 cut(s)	2609, 2839
TaqI	TCGA	4 cut(s)	2326, 2389, 3142, 3301
TatI	WGTACW	2 cut(s)	734, 2479
TauI	GCSGC	1 cut(s)	3287
TfiI	GAWTC	4 cut(s)	1006, 1154, 2585, 2973
TseFI	GTSAC	6 cut(s)	1062, 1548, 1690, 2378, 2650, 3371
Tsp45I	GTSAC	6 cut(s)	1062, 1548, 1690, 2378, 2650, 3371
TspGWI	ACGGA	3 cut(s)	1331, 1335, 1391
Van91I	CCANNNNNTGG	5 cut(s)	311, 712, 875, 3068, 3650
Vha464I	CTTAAG	1 cut(s)	2912
VneI	GTGCAC	1 cut(s)	243
VpaK11BI	GGWCC	2 cut(s)	1350, 2854
VspI	ATTAAT	1 cut(s)	1425
XapI	RAATTY	4 cut(s)	142, 1741, 1978, 3668
XbaI	TCTAGA	1 cut(s)	2722
XceI	RCATGY	2 cut(s)	95, 269
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2318, 3065
XmiI	GTMKAC	2 cut(s)	2730, 3110
XspI	CTAG	9 cut(s)	326, 810, 1767, 1902, 2681, 2723, 2807, 2966, 3234
ZrmI	AGTACT	1 cut(s)	2481
Zsp2I	ATGCAT	3 cut(s)	103, 1277, 1575

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.