MD05G1001700.v1.1

glycosyltransferase At5g03795

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000727
Physical Location & Seq
Reverse (-)
843092 .. 847735
4644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1001700.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGTGTGGATCAATGGTACAGAATTTGATGAAACCAATGGGACGTCGGCTATAATAACTAGTACACCAATAAAGAGATATAGCAGGTTGGAGAAACTTGAAGCAAATTTGGCCGGAGTTCGGGCTTCCATAAGAGAAGCTGCTCGAGTTCGAAATCTAACTTCTACCCATGAAGATCCAGACTATGTTCCTCATGGACCAATTTACAGGAATGCAAATGCTTTTCACAGGAGTTACTTGGAGATGGAAAAGCATTTCAAGATATTTGTATACGGAGAAGGAGAGCCACCAATATTTCACAACGGTCCATGCAAAAGCATATATTCGACAGAAGGGAGATCTATCCATGAAATGGAAATGGAGAATGTTTACAGAACAAGAGATCCAGATGAGGCCCTCGTTTATTTTCTTCCCTTCAGCGTGGTGATGTTGGTGCAGTACCTGTATGTGGCCGACTCCCATGACACTGAGCCCATCGGACGAGCTGTCATCGACTATGTCAATGTCATTTCTGATAAGCATCCCTTTTGGAATCGAAGCCTTGGTGCTGATCACTTTATGATTTCTTGTCACCATTGGGGGCCGAGCACATCTGCTTATGTTCCTAATTTATACCACAACTCCATAAGAGTGTTATGCAATGCAAACACATCTGAAGGATTCAACCCCTCAAAAGATGTCTCATTTCCGGAAATCCATCTCCGAACTGGGGAAACCGAAGGACTTCTCGGTGGTCTCTCCCCATCTCAAAGGTCGATTCTTGCATTCTTCGCAGGCCGGCTAAGCGGCCACATAAGGTACCTGCTTCTAAACGAATGGAAAGAAAAAGACCAAGACGTGCAAGTTTACGACCAATTCCCCAACGGAGTATCCTATGAATCAATGCTGAAGAAGAACAGGTTCTGCTTGTGCCCTAGTGGTTACGAAGTAGCAAGCCCGAGAGTGGTGGAAGCCATATACGCAGAGTGTGTTCCAGTGTTGATTTCGGACAGCTATGTTCCGCCGTTCAGCGATGTTCTGGAGTGGAAGTCGTTTTCTGTGCAAGTGAAAGTGAAGGACATACCAAACATCAAAAGGATACTGAAGGGAATATCACAAAGTCAGTACTTGAGAATGCAGAGGAAAGTGAAGCAGGTGCAGAGGCATTTTGTGGTTCACTGGAAAGCTTGGTGCAATGGCTTCAAGTTTCGGAACTGGTGCAGATTCGATGTTTTCCATATGATTGTTCATTCCATTTGGCTCAGGAGGTTGAACATCCGCATTGAGGACGATCCAGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

428

Amino Acids

49.4

Weight (kDa)

7.29

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 83 - 362 1.1e-58 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1123
AatII GACGTC 1 cut(s) 47
Acc36I ACCTGC 3 cut(s) 75, 810, 1123
Acc65I GGTACC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 798
AccB7I CCANNNNNTGG 1 cut(s) 352
AccI GTMKAC 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 688
AciI CCGC 3 cut(s) 786, 1001, 1258
AclWI GGATC 4 cut(s) 17, 170, 377, 1265
AcoI YGGCCR 3 cut(s) 111, 450, 787
AcsI RAATTY 2 cut(s) 23, 106
AcuI CTGAAG 4 cut(s) 400, 675, 908, 1103
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 5 cut(s) 19, 64, 440, 800, 1106
AfiI CCNNNNNNNGG 6 cut(s) 120, 352, 448, 709, 943, 1264
AgsI TTSAA 5 cut(s) 101, 259, 664, 1183, 1252
AhdI GACNNNNNGTC 1 cut(s) 485
AhlI ACTAGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 838
AloI GAACNNNNNNTCC 2 cut(s) 367, 399
AluBI AGCT 4 cut(s) 140, 485, 993, 1166
AluI AGCT 4 cut(s) 140, 485, 993, 1166
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 2 cut(s) 685, 740
AlwI GGATC 4 cut(s) 17, 170, 377, 1265
AlwNI CAGNNNCTG 2 cut(s) 442, 903
Ama87I CYCGRG 2 cut(s) 144, 937
Aor13HI TCCGGA 1 cut(s) 688
AoxI GGCC 6 cut(s) 111, 393, 450, 581, 775, 787
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 23, 106
Asp700I GAANNNNTTC 2 cut(s) 723, 899
Asp718I GGTACC 1 cut(s) 798
AspS9I GGNCC 4 cut(s) 197, 305, 394, 581
AsuHPI GGTGA 2 cut(s) 436, 563
AsuII TTCGAA 1 cut(s) 151
AvaI CYCGRG 2 cut(s) 144, 937
AvaII GGWCC 2 cut(s) 197, 305
BaeGI GKGCMC 1 cut(s) 914
BanI GGYRCC 1 cut(s) 798
BanII GRGCYC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 590
BbvI GCAGC 1 cut(s) 127
BccI CCATC 4 cut(s) 238, 482, 705, 751
BceAI ACGGC 1 cut(s) 988
BciVI GTATCC 2 cut(s) 880, 1071
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 685, 740
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 2 cut(s) 60, 915
BfuAI ACCTGC 3 cut(s) 75, 810, 1123
BfuI GTATCC 2 cut(s) 880, 1071
BglII AGATCT 1 cut(s) 338
BisI GCNGC 2 cut(s) 141, 787
BlpI GCTNAGC 1 cut(s) 782
BlsI GCNGC 2 cut(s) 142, 788
BmcAI AGTACT 1 cut(s) 1106
Bme18I GGWCC 2 cut(s) 197, 305
BmeRI GACNNNNNGTC 1 cut(s) 485
BmeT110I CYCGRG 2 cut(s) 144, 937
BmgBI CACGTC 1 cut(s) 838
BmgT120I GGNCC 4 cut(s) 197, 305, 394, 581
BmiI GGNNCC 2 cut(s) 582, 800
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 1 cut(s) 529
BmuI ACTGGG 1 cut(s) 717
BpmI CTGGAG 1 cut(s) 1040
Bpu10I CCTNAGC 1 cut(s) 1241
Bpu1102I GCTNAGC 1 cut(s) 782
Bpu14I TTCGAA 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 1129
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 1 cut(s) 541
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 4 cut(s) 605, 635, 1013, 1043
Bsc4I CCNNNNNNNGG 6 cut(s) 120, 352, 448, 709, 943, 1264
Bse118I RCCGGY 1 cut(s) 777
Bse1I ACTGG 5 cut(s) 712, 974, 1163, 1199, 1274
Bse3DI GCAATG 2 cut(s) 646, 1180
Bse8I GATNNNNATC 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 688
BseDI CCNNGG 1 cut(s) 541
BseGI GGATG 2 cut(s) 520, 1254
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 6 cut(s) 120, 352, 448, 709, 943, 1264
BseMI GCAATG 2 cut(s) 646, 1180
BseMII CTCAG 2 cut(s) 459, 1255
BseNI ACTGG 5 cut(s) 712, 974, 1163, 1199, 1274
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 3 cut(s) 455, 1157, 1219
BshFI GGCC 6 cut(s) 113, 395, 452, 583, 777, 789
BshNI GGYRCC 1 cut(s) 798
BsiHKAI GWGCWC 1 cut(s) 590
BsiHKCI CYCGRG 2 cut(s) 144, 937
BsiSI CCGG 3 cut(s) 114, 689, 778
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 6 cut(s) 120, 352, 448, 709, 943, 1264
BsmAI GTCTC 2 cut(s) 685, 740
BsmFI GGGAC 1 cut(s) 55
BsmI GAATGC 3 cut(s) 217, 764, 1119
BsnI GGCC 6 cut(s) 113, 395, 452, 583, 777, 789
Bso31I GGTCTC 1 cut(s) 740
BsoBI CYCGRG 2 cut(s) 144, 937
Bsp119I TTCGAA 1 cut(s) 151
Bsp1286I GDGCHC 3 cut(s) 474, 590, 914
Bsp13I TCCGGA 1 cut(s) 688
Bsp143I GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
Bsp1720I GCTNAGC 1 cut(s) 782
BspACI CCGC 3 cut(s) 786, 1001, 1258
BspANI GGCC 6 cut(s) 113, 395, 452, 583, 777, 789
BspCNI CTCAG 2 cut(s) 460, 1254
BspEI TCCGGA 1 cut(s) 688
BspLI GGNNCC 2 cut(s) 582, 800
BspMI ACCTGC 3 cut(s) 75, 810, 1123
BspPI GGATC 4 cut(s) 17, 170, 377, 1265
BspT104I TTCGAA 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 798
BspTNI GGTCTC 1 cut(s) 740
BsrDI GCAATG 2 cut(s) 646, 1180
BsrFI RCCGGY 1 cut(s) 777
BsrI ACTGG 5 cut(s) 712, 974, 1163, 1199, 1274
BssAI RCCGGY 1 cut(s) 777
BssECI CCNNGG 1 cut(s) 541
BssMI GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
BssNAI GTATAC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 541
Bst1107I GTATAC 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 305
BstACI GRCGYC 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 151
BstC8I GCNNGC 3 cut(s) 775, 779, 934
BstDEI CTNAG 3 cut(s) 468, 782, 1241
BstF5I GGATG 2 cut(s) 520, 1254
BstKTI GATC 6 cut(s) 12, 178, 341, 385, 553, 1273
BstMAI GTCTC 2 cut(s) 685, 740
BstMBI GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
BstMWI GCNNNNNNNGC 4 cut(s) 110, 770, 783, 959
BstSLI GKGCMC 1 cut(s) 914
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 3 cut(s) 175, 338, 382
BstYI RGATCY 3 cut(s) 175, 338, 382
BstZ17I GTATAC 1 cut(s) 271
BsuI GTATCC 2 cut(s) 880, 1071
BsuRI GGCC 6 cut(s) 113, 395, 452, 583, 777, 789
BtgZI GCGATG 1 cut(s) 1026
BtrI CACGTC 1 cut(s) 838
BtsCI GGATG 2 cut(s) 520, 1254
BtsIMutI CAGTG 3 cut(s) 465, 981, 1156
BveI ACCTGC 3 cut(s) 75, 810, 1123
Cac8I GCNNGC 3 cut(s) 775, 779, 934
CaiI CAGNNNCTG 2 cut(s) 442, 903
Cfr10I RCCGGY 1 cut(s) 777
Cfr13I GGNCC 4 cut(s) 197, 305, 394, 581
Csp6I GTAC 5 cut(s) 18, 63, 439, 799, 1105
CviAII CATG 5 cut(s) 170, 194, 309, 347, 461
CviQI GTAC 5 cut(s) 18, 63, 439, 799, 1105
DdeI CTNAG 3 cut(s) 468, 782, 1241
DpnI GATC 6 cut(s) 11, 177, 340, 384, 552, 1272
DpnII GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
DriI GACNNNNNGTC 1 cut(s) 485
EaeI YGGCCR 3 cut(s) 111, 450, 787
Eam1105I GACNNNNNGTC 1 cut(s) 485
EciI GGCGGA 1 cut(s) 990
Eco130I CCWWGG 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 740
Eco47I GGWCC 2 cut(s) 197, 305
Eco57I CTGAAG 4 cut(s) 400, 675, 908, 1103
Eco88I CYCGRG 2 cut(s) 144, 937
EcoO109I RGGNCCY 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 474
ErhI CCWWGG 1 cut(s) 541
FaeI CATG 5 cut(s) 173, 197, 312, 350, 464
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FaqI GGGAC 1 cut(s) 55
FatI CATG 5 cut(s) 169, 193, 308, 346, 460
FauNDI CATATG 1 cut(s) 1218
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 141, 787
FokI GGATG 2 cut(s) 507, 1241
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 2 cut(s) 141, 787
FspBI CTAG 2 cut(s) 60, 915
GluI GCNGC 2 cut(s) 141, 787
GsuI CTGGAG 1 cut(s) 1040
HaeIII GGCC 6 cut(s) 113, 395, 452, 583, 777, 789
HapII CCGG 3 cut(s) 114, 689, 778
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 5 cut(s) 173, 197, 312, 350, 464
HindIII AAGCTT 1 cut(s) 1164
HinfI GANTC 6 cut(s) 455, 532, 660, 757, 878, 1203
HpaII CCGG 3 cut(s) 114, 689, 778
HphI GGTGA 2 cut(s) 436, 563
Hpy166II GTNNAC 5 cut(s) 65, 271, 370, 847, 1156
Hpy188I TCNGA 6 cut(s) 479, 514, 655, 704, 988, 1191
Hpy188III TCNNGA 6 cut(s) 179, 259, 386, 689, 1019, 1243
Hpy8I GTNNAC 5 cut(s) 65, 271, 370, 847, 1156
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 7 cut(s) 272, 326, 424, 650, 713, 1048, 1078
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 2 cut(s) 44, 837
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 770, 783, 959
HpyF3I CTNAG 3 cut(s) 468, 782, 1241
HpySE526I ACGT 2 cut(s) 44, 837
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 5 cut(s) 173, 197, 312, 350, 464
Kpn2I TCCGGA 1 cut(s) 688
KpnI GGTACC 1 cut(s) 802
KroI GCCGGC 1 cut(s) 777
KroNI GCCGGC 1 cut(s) 779
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 529
MaeI CTAG 2 cut(s) 60, 915
MaeII ACGT 2 cut(s) 44, 837
MaeIII GTNAC 3 cut(s) 233, 569, 920
MalI GATC 6 cut(s) 11, 177, 340, 384, 552, 1272
MboI GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
MboII GAAGA 5 cut(s) 185, 401, 760, 901, 904
MflI RGATCY 3 cut(s) 175, 338, 382
MhlI GDGCHC 3 cut(s) 474, 590, 914
MluCI AATT 5 cut(s) 23, 106, 201, 607, 854
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 8 cut(s) 201, 385, 407, 679, 1113, 1134, 1239, 1258
MroI TCCGGA 1 cut(s) 688
MroNI GCCGGC 1 cut(s) 777
MroXI GAANNNNTTC 2 cut(s) 723, 899
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 3 cut(s) 114, 689, 778
Mva1269I GAATGC 3 cut(s) 217, 764, 1119
MwoI GCNNNNNNNGC 4 cut(s) 110, 770, 783, 959
NaeI GCCGGC 1 cut(s) 779
NdeI CATATG 1 cut(s) 1218
NdeII GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
NgoMIV GCCGGC 1 cut(s) 777
NlaIII CATG 5 cut(s) 173, 197, 312, 350, 464
NlaIV GGNNCC 2 cut(s) 582, 800
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 569
NspV TTCGAA 1 cut(s) 151
PaeR7I CTCGAG 1 cut(s) 144
PaqCI CACCTGC 1 cut(s) 1123
PctI GAATGC 3 cut(s) 217, 764, 1119
PdiI GCCGGC 1 cut(s) 779
PdmI GAANNNNTTC 2 cut(s) 723, 899
PfeI GAWTC 5 cut(s) 532, 660, 757, 878, 1203
PflFI GACNNNGTC 1 cut(s) 497
PflMI CCANNNNNTGG 1 cut(s) 352
PkrI GCNGC 2 cut(s) 142, 788
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PspN4I GGNNCC 2 cut(s) 582, 800
PspPI GGNCC 4 cut(s) 197, 305, 394, 581
PspXI VCTCGAGB 1 cut(s) 144
PstNI CAGNNNCTG 2 cut(s) 442, 903
PsuI RGATCY 3 cut(s) 175, 338, 382
PsyI GACNNNGTC 1 cut(s) 497
RsaI GTAC 5 cut(s) 19, 64, 440, 800, 1106
RsaNI GTAC 5 cut(s) 18, 63, 439, 799, 1105
RseI CAYNNNNRTG 1 cut(s) 629
SatI GCNGC 2 cut(s) 141, 787
Sau3AI GATC 6 cut(s) 9, 175, 338, 382, 550, 1270
Sau96I GGNCC 4 cut(s) 197, 305, 394, 581
ScaI AGTACT 1 cut(s) 1106
SchI GAGTC 1 cut(s) 449
SduI GDGCHC 3 cut(s) 474, 590, 914
SfaNI GCATC 1 cut(s) 529
Sfr274I CTCGAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 151
SinI GGWCC 2 cut(s) 197, 305
SlaI CTCGAG 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 629
SmlI CTYRAG 2 cut(s) 144, 1108
SmoI CTYRAG 2 cut(s) 144, 1108
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 5 cut(s) 23, 106, 201, 607, 854
SsiI CCGC 3 cut(s) 786, 1001, 1258
SspI AATATT 1 cut(s) 294
SspMI CTAG 2 cut(s) 60, 915
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 2 cut(s) 47, 840
TaqI TCGA 7 cut(s) 145, 151, 326, 492, 535, 755, 1206
TasI AATT 5 cut(s) 23, 106, 201, 607, 854
TatI WGTACW 2 cut(s) 62, 1104
TauI GCSGC 1 cut(s) 789
TfiI GAWTC 5 cut(s) 532, 660, 757, 878, 1203
TscAI CASTG 3 cut(s) 472, 981, 1163
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 5 cut(s) 45, 186, 363, 891, 1217
TspGWI ACGGA 2 cut(s) 288, 879
TspRI CASTG 3 cut(s) 472, 981, 1163
Tth111I GACNNNGTC 1 cut(s) 497
Van91I CCANNNNNTGG 1 cut(s) 352
VpaK11BI GGWCC 2 cut(s) 197, 305
XapI RAATTY 2 cut(s) 23, 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 704
XhoI CTCGAG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 2 cut(s) 723, 899
XspI CTAG 2 cut(s) 60, 915
ZraI GACGTC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.