pycom15g12510

glycosyltransferase At5g03795

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000727
Physical Location & Seq
Forward (+)
8534772 .. 8535305
534 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12510.1

Sequence Viewer

Length: 534 bp
ATGCCAAGAATTGGCGGTCAGCCTCCATCGAAGCGATCCACACTAGCTTTCTTTGCAGGGCGTCTACACGGCCACATTAGGTACCTTCTCCTAAACGAATGGAAAGGCAAAGACCAAGATGTGCAAGTTTATGACCAACTTCCTCAGGGCGTGTCGTACGAGAACATGCTGAAGAGTAGCAAGTTTTGCTTATGCCCTAGCGGGTACGAAGTGGCAAGTCCAAGAGTGGTGGAAGTAATTTATGCCGAGTGTGTGCCAGTGTTGATTTCGGATGGTTACGTTCCTCCATTCAGCGATGTTTTGAACTGGAAGTCATTTTCTGTACAAGTTCCGGTCAAGAATATATCCGACATCAAGACCATATTGACAGCCATATCGAGCAGACAGTACCTCCGAATGCAGAGGAGGGTGAAACAAGTGCAACGACATTTTTTGGTGAATGGACCCCCGAAGAGATACGATGCTTTCCACATGATTGTTCACTCCATCTGGCTCAGACGCTTAAACATTCGCATTCAAGATGTATTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

178

Amino Acids

20.46

Weight (kDa)

9.83

Isoelectric Point (pI)

59.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 6 - 124 2.7e-36 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 81
AccB7I CCANNNNNTGG 1 cut(s) 11
AccI GTMKAC 1 cut(s) 64
AciI CCGC 2 cut(s) 15, 201
AclWI GGATC 1 cut(s) 30
AcoI YGGCCR 1 cut(s) 70
AcuI CTGAAG 1 cut(s) 191
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 5 cut(s) 83, 158, 206, 324, 389
AfiI CCNNNNNNNGG 1 cut(s) 11
AgsI TTSAA 2 cut(s) 304, 518
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
AlwI GGATC 1 cut(s) 30
AoxI GGCC 1 cut(s) 70
Asp718I GGTACC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 443
AsuHPI GGTGA 3 cut(s) 421, 448, 520
AvaII GGWCC 1 cut(s) 443
AxyI CCTNAGG 1 cut(s) 144
BanI GGYRCC 1 cut(s) 81
BccI CCATC 3 cut(s) 34, 266, 494
BceAI ACGGC 1 cut(s) 85
BfaI CTAG 2 cut(s) 44, 198
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 2 cut(s) 83, 445
BmsI GCATC 1 cut(s) 451
BsaHI GRCGYC 1 cut(s) 61
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 257, 311
Bse21I CCTNAGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 11
BseMII CTCAG 2 cut(s) 158, 508
BseNI ACTGG 2 cut(s) 257, 311
BseRI GAGGAG 1 cut(s) 418
BshFI GGCC 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 332
BsiWI CGTACG 1 cut(s) 156
BslI CCNNNNNNNGG 1 cut(s) 11
BsmI GAATGC 2 cut(s) 402, 513
BsnI GGCC 1 cut(s) 72
Bsp1407I TGTACA 1 cut(s) 322
Bsp143I GATC 1 cut(s) 35
BspACI CCGC 2 cut(s) 15, 201
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 2 cut(s) 157, 507
BspLI GGNNCC 2 cut(s) 83, 445
BspPI GGATC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 322
BsrI ACTGG 2 cut(s) 257, 311
BssMI GATC 1 cut(s) 35
BssNI GRCGYC 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 2 cut(s) 167, 446
BstACI GRCGYC 1 cut(s) 61
BstAPI GCANNNNNTGC 1 cut(s) 186
BstAUI TGTACA 1 cut(s) 322
BstDEI CTNAG 2 cut(s) 144, 494
BstF5I GGATG 1 cut(s) 277
BstKTI GATC 1 cut(s) 38
BstMBI GATC 1 cut(s) 35
BstMWI GCNNNNNNNGC 2 cut(s) 53, 186
BstNSI RCATGY 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 443
CseI GACGC 2 cut(s) 50, 507
Csp6I GTAC 5 cut(s) 82, 157, 205, 323, 388
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviAII CATG 2 cut(s) 166, 472
CviJI RGCY 5 cut(s) 22, 47, 72, 371, 493
CviKI_1 RGCY 5 cut(s) 22, 47, 72, 371, 493
CviQI GTAC 5 cut(s) 82, 157, 205, 323, 388
DdeI CTNAG 2 cut(s) 144, 494
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 2 cut(s) 167, 446
EarI CTCTTC 2 cut(s) 167, 446
Eco47I GGWCC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 191
Eco81I CCTNAGG 1 cut(s) 144
FaeI CATG 2 cut(s) 169, 475
FaiI YATR 8 cut(s) 132, 167, 193, 243, 344, 362, 374, 473
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FatI CATG 2 cut(s) 165, 471
FauI CCCGC 1 cut(s) 194
FblI GTMKAC 1 cut(s) 64
FokI GGATG 1 cut(s) 284
FspBI CTAG 2 cut(s) 44, 198
HaeIII GGCC 1 cut(s) 72
HapII CCGG 1 cut(s) 332
HgaI GACGC 2 cut(s) 50, 507
Hin1I GRCGYC 1 cut(s) 61
Hin1II CATG 2 cut(s) 169, 475
HpaII CCGG 1 cut(s) 332
HphI GGTGA 3 cut(s) 421, 448, 520
Hpy166II GTNNAC 2 cut(s) 65, 481
Hpy188I TCNGA 4 cut(s) 271, 349, 395, 497
Hpy188III TCNNGA 3 cut(s) 337, 355, 518
Hpy8I GTNNAC 2 cut(s) 65, 481
HpyAV CCTTC 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 56, 124, 400, 421
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 186
HpyF3I CTNAG 2 cut(s) 144, 494
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 1 cut(s) 61
Hsp92II CATG 2 cut(s) 169, 475
KpnI GGTACC 1 cut(s) 85
Kzo9I GATC 1 cut(s) 35
LpnPI CCDG 6 cut(s) 42, 131, 270, 292, 345, 475
LweI GCATC 1 cut(s) 451
MaeI CTAG 2 cut(s) 44, 198
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 275
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MboII GAAGA 2 cut(s) 184, 463
MluCI AATT 2 cut(s) 9, 237
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 33, 153, 294, 396, 399, 401
MseI TTAA 1 cut(s) 503
MspI CCGG 1 cut(s) 332
Mva1269I GAATGC 2 cut(s) 402, 513
MwoI GCNNNNNNNGC 2 cut(s) 53, 186
NdeII GATC 1 cut(s) 35
NlaIII CATG 2 cut(s) 169, 475
NlaIV GGNNCC 2 cut(s) 83, 445
NmeAIII GCCGAG 1 cut(s) 271
NspI RCATGY 1 cut(s) 169
PctI GAATGC 2 cut(s) 402, 513
Pfl23II CGTACG 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 11
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 83, 445
PspPI GGNCC 1 cut(s) 443
RsaI GTAC 5 cut(s) 83, 158, 206, 324, 389
RsaNI GTAC 5 cut(s) 82, 157, 205, 323, 388
SaqAI TTAA 1 cut(s) 503
Sau3AI GATC 1 cut(s) 35
Sau96I GGNCC 1 cut(s) 443
SetI ASST 6 cut(s) 49, 83, 87, 282, 393, 533
SfaNI GCATC 1 cut(s) 451
SinI GGWCC 1 cut(s) 443
Sse9I AATT 2 cut(s) 9, 237
SsiI CCGC 2 cut(s) 15, 201
SspMI CTAG 2 cut(s) 44, 198
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 29, 377
TasI AATT 2 cut(s) 9, 237
TatI WGTACW 1 cut(s) 322
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 1 cut(s) 264
TspRI CASTG 1 cut(s) 264
Van91I CCANNNNNTGG 1 cut(s) 11
VpaK11BI GGWCC 1 cut(s) 443
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 64
XspI CTAG 2 cut(s) 44, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.