MD15G1138400.v1.1

glycosyltransferase At5g03795

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000727
Physical Location & Seq
Forward (+)
10157716 .. 10162353
4638 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1138400.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGAGGCCCAGTCGTAACAAACCAGTTTCATCTTTATCCTCGATTTTAGTGGGATTAATACTCGTATTAACTTTGGCAGTATCTTTGTTGGTAATCTCAAGGGCTTCTTCCTCCTCCTCCTCATCTTCGTCTTCTTCAGAGACAACATGGAAAGTAGCTGCAGCTTCAGCTGGTGGTAGTAGTGATATTAATACTACTACTACTACTTACTCTCATGCTGCTTTAACATCCACTCAACCACCACCACAACCTCTACTTTTTAGTTTCTCTTCACTAAATAACCTAAATACAGCCAACTCATCTCCAGCTCCTGCTCCTGCCCCTCAAATATCAGCAGCTGATGTGTTGCAAGAGACGATAATCCAGAAAGTAGAAGGAGACGTGATTAATACAGTGGATAATGATGTTGTTGCCAATAGAACAGTGTTCATCAGCGGCAGCCAGCTTGATAAAGTAGAAGCAAGTTTGGGTAGAGCACGGTCTGCCATTAGAGAAGCTGCTAAAGTTAGGAATAAAACATCCACACATCAAGACCCTGATTATGTTCCTCGAGGCCCTATATATCATAACCCTAACGCCTTCCACAGGAGTTACCTAGAGATGGAGAAGGTGTTCAAGATCTATGTATACGAAGAAGGAGAGGTTCCAATATTTCACGAAGGGCCATGCAGGGGTATATATTCATCAGAAGGAAGGTTTATTCATGAGATGGAGAAGGGCAAATTTTATCGTACAAAAGACCCTCACAAGGCACTTGTTTATTTCCTTCCATTCAGTGTGGTTACTATGGTAGAGTATATCTATATGCCTGAAACCTTTGATCGAAGTGGCATGAAGCTTGCTGTGGTCGACTACATCAATCTCATTTCTCACAAACATCCGTTCTGGAACCGAAGCCACGGTGCCGATCATTTCATGTTGTCCTGTCATGATTGGGGACCATATACGACATCCTTCGTCCCTGATTTATTCCACAACTCCATTAGAATTCTATGCAATGCGAACACTACAGAAGGATTTAATCCGTCGAAAGATGCGTCACTTCCAGAGATTAATCTGAAAACATCGGAAATGTCGGGAATCGGCGGCCAGCCTCCATCGAAGCGATCCACACTAGCTTTCTTTGCAGGGGGTCTACACGGCCACATCAGGTACCTTCTCCTAAACGAATGGAAGGGCAAAGACCAAGACGTGTTAGTTTACGACAAACTTCCTCAGGGAGTGTCGTACGAGAACATGCTCAAGAGCAGCAAGTTTTGCTTATGCCCTAGCGGGTACGAAGTGGCAAGTCCAAGAGTGGTGGAAGCAATTTATGCACAGTGTGTGCCGGTGTTGATTTCAGATGGCTACGTTCCTCCATTCAGCGATGTTTTGAACTGGAACTCGTTTTCTGTACAAGTTCCGGTCAAGAATATATCCGACATCAAGACCATATTGACAGCCATATCGAGCAGGGAGTACCTCCGGATGCAGAGGAAGGTGAAACAAGTGCAACGACATTTTTTGGTGAATGGACCCCCGAAGAGATACGATGTTTTCCACATGATTGTTCACTCCATCTGGCTCAGACGCTTAAACATTCGCATTCAAGATGTATTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

535

Amino Acids

59.54

Weight (kDa)

8.88

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 202 - 481 4.9e-61 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 549
Acc65I GGTACC 1 cut(s) 1152
AccB1I GGYRCC 2 cut(s) 902, 1152
AccI GTMKAC 3 cut(s) 627, 849, 1135
AccIII TCCGGA 1 cut(s) 1464
AciI CCGC 3 cut(s) 435, 1086, 1272
AclWI GGATC 1 cut(s) 1101
AcoI YGGCCR 2 cut(s) 1087, 1141
AcsI RAATTY 2 cut(s) 722, 987
AcuI CTGAAG 2 cut(s) 120, 150
AdeI CACNNNGTG 1 cut(s) 1322
AfaI GTAC 6 cut(s) 733, 1154, 1229, 1277, 1395, 1460
AfiI CCNNNNNNNGG 4 cut(s) 585, 601, 671, 748
AflIII ACRYGT 1 cut(s) 1191
AgsI TTSAA 3 cut(s) 616, 1375, 1589
AjiI CACGTC 2 cut(s) 382, 1192
AloI GAACNNNNNNTCC 2 cut(s) 596, 628
AluBI AGCT 9 cut(s) 158, 164, 170, 308, 338, 445, 497, 838, 1118
AluI AGCT 9 cut(s) 158, 164, 170, 308, 338, 445, 497, 838, 1118
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 3 cut(s) 134, 347, 372
AlwI GGATC 1 cut(s) 1101
AlwNI CAGNNNCTG 2 cut(s) 311, 338
Ama87I CYCGRG 1 cut(s) 549
Aor13HI TCCGGA 1 cut(s) 1464
AoxI GGCC 5 cut(s) 5, 553, 662, 1087, 1141
ApeKI GCWGC 7 cut(s) 158, 161, 218, 335, 438, 497, 1248
ApoI RAATTY 2 cut(s) 722, 987
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
AseI ATTAAT 4 cut(s) 56, 189, 387, 1053
Asp700I GAANNNNTTC 1 cut(s) 611
Asp718I GGTACC 1 cut(s) 1152
AspS9I GGNCC 5 cut(s) 6, 554, 662, 938, 1514
AsuHPI GGTGA 3 cut(s) 1492, 1519, 1591
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 2 cut(s) 938, 1514
AxyI CCTNAGG 1 cut(s) 1215
BanI GGYRCC 2 cut(s) 902, 1152
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 7 cut(s) 145, 173, 205, 347, 450, 484, 1260
BccI CCATC 5 cut(s) 595, 703, 1105, 1337, 1565
BceAI ACGGC 1 cut(s) 1156
BcoDI GTCTC 3 cut(s) 134, 347, 372
BfaI CTAG 3 cut(s) 596, 1115, 1269
BfmI CTRYAG 2 cut(s) 159, 1008
BglII AGATCT 1 cut(s) 618
BisI GCNGC 9 cut(s) 159, 162, 219, 336, 436, 439, 498, 1087, 1249
BlsI GCNGC 9 cut(s) 160, 163, 220, 337, 437, 440, 499, 1088, 1250
Bme18I GGWCC 2 cut(s) 938, 1514
BmeT110I CYCGRG 1 cut(s) 549
BmgBI CACGTC 2 cut(s) 382, 1192
BmgT120I GGNCC 5 cut(s) 6, 554, 662, 938, 1514
BmiI GGNNCC 6 cut(s) 645, 890, 904, 939, 1154, 1516
BmrI ACTGGG 1 cut(s) 3
BmsI GCATC 2 cut(s) 1024, 1458
BmuI ACTGGG 1 cut(s) 3
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 82, 1226
BsaJI CCNNGG 1 cut(s) 898
BsaWI WCCGGW 2 cut(s) 1402, 1464
BsaXI ACNNNNNCTCC 2 cut(s) 596, 626
Bsc4I CCNNNNNNNGG 4 cut(s) 585, 601, 671, 748
Bse118I RCCGGY 1 cut(s) 1327
Bse1I ACTGG 3 cut(s) 9, 23, 1382
Bse21I CCTNAGG 1 cut(s) 1215
Bse3DI GCAATG 1 cut(s) 1003
BseAI TCCGGA 1 cut(s) 1464
BseDI CCNNGG 1 cut(s) 898
BseGI GGATG 5 cut(s) 227, 518, 877, 950, 1473
BseLI CCNNNNNNNGG 4 cut(s) 585, 601, 671, 748
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 2 cut(s) 1229, 1579
BseNI ACTGG 3 cut(s) 9, 23, 1382
BseRI GAGGAG 3 cut(s) 103, 106, 109
BseXI GCAGC 7 cut(s) 145, 173, 205, 347, 450, 484, 1260
BshFI GGCC 5 cut(s) 7, 555, 664, 1089, 1143
BshNI GGYRCC 2 cut(s) 902, 1152
BsiHKAI GWGCWC 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 3 cut(s) 1328, 1403, 1465
BsiWI CGTACG 1 cut(s) 1227
BslFI GGGAC 2 cut(s) 944, 951
BslI CCNNNNNNNGG 4 cut(s) 585, 601, 671, 748
BsmAI GTCTC 3 cut(s) 134, 347, 372
BsmBI CGTCTC 2 cut(s) 347, 372
BsmFI GGGAC 2 cut(s) 944, 951
BsmI GAATGC 1 cut(s) 1584
BsnI GGCC 5 cut(s) 7, 555, 664, 1089, 1143
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 1 cut(s) 478
Bsp13I TCCGGA 1 cut(s) 1464
Bsp1407I TGTACA 1 cut(s) 1393
Bsp143I GATC 4 cut(s) 618, 820, 907, 1106
BspACI CCGC 3 cut(s) 435, 1086, 1272
BspANI GGCC 5 cut(s) 7, 555, 664, 1089, 1143
BspCNI CTCAG 2 cut(s) 1228, 1578
BspEI TCCGGA 1 cut(s) 1464
BspHI TCATGA 2 cut(s) 703, 928
BspLI GGNNCC 6 cut(s) 645, 890, 904, 939, 1154, 1516
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 1101
BspT107I GGYRCC 2 cut(s) 902, 1152
BsrDI GCAATG 1 cut(s) 1003
BsrFI RCCGGY 1 cut(s) 1327
BsrGI TGTACA 1 cut(s) 1393
BsrI ACTGG 3 cut(s) 9, 23, 1382
BssAI RCCGGY 1 cut(s) 1327
BssECI CCNNGG 1 cut(s) 898
BssMI GATC 4 cut(s) 618, 820, 907, 1106
BssNAI GTATAC 1 cut(s) 628
Bst1107I GTATAC 1 cut(s) 628
Bst4CI ACNGT 5 cut(s) 394, 424, 480, 902, 1320
Bst6I CTCTTC 2 cut(s) 274, 1517
BstAPI GCANNNNNTGC 3 cut(s) 482, 1257, 1313
BstAUI TGTACA 1 cut(s) 1393
BstC8I GCNNGC 3 cut(s) 443, 840, 1091
BstDEI CTNAG 2 cut(s) 1215, 1565
BstDSI CCRYGG 1 cut(s) 898
BstENI CCTNNNNNAGG 1 cut(s) 583
BstF5I GGATG 5 cut(s) 227, 518, 877, 950, 1473
BstKTI GATC 4 cut(s) 621, 823, 910, 1109
BstMAI GTCTC 3 cut(s) 134, 347, 372
BstMBI GATC 4 cut(s) 618, 820, 907, 1106
BstMWI GCNNNNNNNGC 5 cut(s) 167, 482, 1124, 1257, 1313
BstNSI RCATGY 1 cut(s) 1240
BstSFI CTRYAG 2 cut(s) 159, 1008
BstV1I GCAGC 7 cut(s) 145, 173, 205, 347, 450, 484, 1260
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
BstZ17I GTATAC 1 cut(s) 628
Bsu36I CCTNAGG 1 cut(s) 1215
BsuRI GGCC 5 cut(s) 7, 555, 664, 1089, 1143
BtgI CCRYGG 1 cut(s) 898
BtgZI GCGATG 1 cut(s) 1380
BtrI CACGTC 2 cut(s) 382, 1192
BtsCI GGATG 5 cut(s) 227, 518, 877, 950, 1473
BtsIMutI CAGTG 4 cut(s) 399, 429, 781, 1325
Cac8I GCNNGC 3 cut(s) 443, 840, 1091
CaiI CAGNNNCTG 2 cut(s) 311, 338
CciI TCATGA 2 cut(s) 703, 928
Cfr10I RCCGGY 1 cut(s) 1327
Cfr13I GGNCC 5 cut(s) 6, 554, 662, 938, 1514
CseI GACGC 2 cut(s) 1026, 1578
Csp6I GTAC 6 cut(s) 732, 1153, 1228, 1276, 1394, 1459
CspCI CAANNNNNGTGG 2 cut(s) 1281, 1316
CviAII CATG 9 cut(s) 147, 215, 666, 704, 832, 916, 929, 1237, 1543
CviQI GTAC 6 cut(s) 732, 1153, 1228, 1276, 1394, 1459
DdeI CTNAG 2 cut(s) 1215, 1565
DpnI GATC 4 cut(s) 620, 822, 909, 1108
DpnII GATC 4 cut(s) 618, 820, 907, 1106
DraIII CACNNNGTG 1 cut(s) 1322
EaeI YGGCCR 2 cut(s) 1087, 1141
Eam1104I CTCTTC 2 cut(s) 274, 1517
EarI CTCTTC 2 cut(s) 274, 1517
Eco47I GGWCC 2 cut(s) 938, 1514
Eco57I CTGAAG 2 cut(s) 120, 150
Eco81I CCTNAGG 1 cut(s) 1215
Eco88I CYCGRG 1 cut(s) 549
EcoNI CCTNNNNNAGG 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 554
EcoRI GAATTC 1 cut(s) 987
Esp3I CGTCTC 2 cut(s) 347, 372
FaeI CATG 9 cut(s) 150, 218, 669, 707, 835, 919, 932, 1240, 1546
FalI AAGNNNNNCTT 4 cut(s) 91, 123, 1244, 1276
FaqI GGGAC 2 cut(s) 944, 951
FatI CATG 9 cut(s) 146, 214, 665, 703, 831, 915, 928, 1236, 1542
FauI CCCGC 1 cut(s) 1265
FblI GTMKAC 3 cut(s) 627, 849, 1135
Fnu4HI GCNGC 9 cut(s) 159, 162, 219, 336, 436, 439, 498, 1087, 1249
FokI GGATG 5 cut(s) 214, 505, 864, 937, 1480
Fsp4HI GCNGC 9 cut(s) 159, 162, 219, 336, 436, 439, 498, 1087, 1249
FspBI CTAG 3 cut(s) 596, 1115, 1269
GluI GCNGC 9 cut(s) 159, 162, 219, 336, 436, 439, 498, 1087, 1249
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 5 cut(s) 7, 555, 664, 1089, 1143
HapII CCGG 3 cut(s) 1328, 1403, 1465
HgaI GACGC 2 cut(s) 1026, 1578
Hin1II CATG 9 cut(s) 150, 218, 669, 707, 835, 919, 932, 1240, 1546
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 1 cut(s) 1080
HpaII CCGG 3 cut(s) 1328, 1403, 1465
HphI GGTGA 3 cut(s) 1492, 1519, 1591
Hpy166II GTNNAC 5 cut(s) 628, 850, 1136, 1201, 1552
Hpy188I TCNGA 7 cut(s) 139, 688, 1059, 1069, 1342, 1420, 1568
Hpy8I GTNNAC 5 cut(s) 628, 850, 1136, 1201, 1552
Hpy99I CGWCG 1 cut(s) 1030
HpyCH4III ACNGT 5 cut(s) 394, 424, 480, 902, 1320
HpyCH4IV ACGT 3 cut(s) 381, 1191, 1350
HpyCH4V TGCA 8 cut(s) 161, 349, 669, 996, 1127, 1316, 1471, 1492
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 482, 1124, 1257, 1313
HpyF3I CTNAG 2 cut(s) 1215, 1565
HpySE526I ACGT 3 cut(s) 381, 1191, 1350
Hsp92II CATG 9 cut(s) 150, 218, 669, 707, 835, 919, 932, 1240, 1546
Kpn2I TCCGGA 1 cut(s) 1464
KpnI GGTACC 1 cut(s) 1156
Kzo9I GATC 4 cut(s) 618, 820, 907, 1106
LmnI GCTCC 2 cut(s) 313, 319
Lsp1109I GCAGC 7 cut(s) 145, 173, 205, 347, 450, 484, 1260
LweI GCATC 2 cut(s) 1024, 1458
MaeI CTAG 3 cut(s) 596, 1115, 1269
MaeII ACGT 3 cut(s) 381, 1191, 1350
MaeIII GTNAC 4 cut(s) 14, 590, 781, 1038
MalI GATC 4 cut(s) 620, 822, 909, 1108
MboI GATC 4 cut(s) 618, 820, 907, 1106
MboII GAAGA 7 cut(s) 99, 117, 123, 126, 261, 644, 1534
MflI RGATCY 1 cut(s) 618
MhlI GDGCHC 1 cut(s) 478
MluCI AATT 3 cut(s) 722, 987, 1308
MmeI TCCRAC 1 cut(s) 1443
MroI TCCGGA 1 cut(s) 1464
MroXI GAANNNNTTC 1 cut(s) 611
MseI TTAA 8 cut(s) 56, 68, 189, 224, 387, 1020, 1053, 1574
MspA1I CMGCKG 3 cut(s) 170, 338, 435
MspI CCGG 3 cut(s) 1328, 1403, 1465
Mva1269I GAATGC 1 cut(s) 1584
MwoI GCNNNNNNNGC 5 cut(s) 167, 482, 1124, 1257, 1313
NdeII GATC 4 cut(s) 618, 820, 907, 1106
NlaIII CATG 9 cut(s) 150, 218, 669, 707, 835, 919, 932, 1240, 1546
NlaIV GGNNCC 6 cut(s) 645, 890, 904, 939, 1154, 1516
NmuCI GTSAC 1 cut(s) 1038
NspI RCATGY 1 cut(s) 1240
PaeR7I CTCGAG 1 cut(s) 549
PagI TCATGA 2 cut(s) 703, 928
PcsI WCGNNNNNNNCGW 1 cut(s) 1034
PctI GAATGC 1 cut(s) 1584
PdmI GAANNNNTTC 1 cut(s) 611
PfeI GAWTC 1 cut(s) 1080
Pfl23II CGTACG 1 cut(s) 1227
PkrI GCNGC 9 cut(s) 160, 163, 220, 337, 437, 440, 499, 1088, 1250
PshBI ATTAAT 4 cut(s) 56, 189, 387, 1053
PspLI CGTACG 1 cut(s) 1227
PspN4I GGNNCC 6 cut(s) 645, 890, 904, 939, 1154, 1516
PspPI GGNCC 5 cut(s) 6, 554, 662, 938, 1514
PspXI VCTCGAGB 1 cut(s) 549
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 2 cut(s) 311, 338
PsuI RGATCY 1 cut(s) 618
PvuII CAGCTG 2 cut(s) 170, 338
RsaI GTAC 6 cut(s) 733, 1154, 1229, 1277, 1395, 1460
RsaNI GTAC 6 cut(s) 732, 1153, 1228, 1276, 1394, 1459
SalI GTCGAC 1 cut(s) 848
SaqAI TTAA 8 cut(s) 56, 68, 189, 224, 387, 1020, 1053, 1574
SatI GCNGC 9 cut(s) 159, 162, 219, 336, 436, 439, 498, 1087, 1249
Sau3AI GATC 4 cut(s) 618, 820, 907, 1106
Sau96I GGNCC 5 cut(s) 6, 554, 662, 938, 1514
SduI GDGCHC 1 cut(s) 478
SfaNI GCATC 2 cut(s) 1024, 1458
SfcI CTRYAG 2 cut(s) 159, 1008
Sfr274I CTCGAG 1 cut(s) 549
SinI GGWCC 2 cut(s) 938, 1514
SlaI CTCGAG 1 cut(s) 549
SmlI CTYRAG 3 cut(s) 97, 549, 1241
SmoI CTYRAG 3 cut(s) 97, 549, 1241
Sse9I AATT 3 cut(s) 722, 987, 1308
SsiI CCGC 3 cut(s) 435, 1086, 1272
SspI AATATT 1 cut(s) 651
SspMI CTAG 3 cut(s) 596, 1115, 1269
TaaI ACNGT 5 cut(s) 394, 424, 480, 902, 1320
TaiI ACGT 3 cut(s) 384, 1194, 1353
TaqI TCGA 7 cut(s) 41, 550, 823, 849, 1028, 1100, 1448
TasI AATT 3 cut(s) 722, 987, 1308
TatI WGTACW 1 cut(s) 1393
TauI GCSGC 2 cut(s) 438, 1089
TfiI GAWTC 1 cut(s) 1080
Tru1I TTAA 8 cut(s) 56, 68, 189, 224, 387, 1020, 1053, 1574
Tru9I TTAA 8 cut(s) 56, 68, 189, 224, 387, 1020, 1053, 1574
TscAI CASTG 4 cut(s) 399, 429, 781, 1325
TseFI GTSAC 1 cut(s) 1038
TseI GCWGC 7 cut(s) 158, 161, 218, 335, 438, 497, 1248
Tsp45I GTSAC 1 cut(s) 1038
TspDTI ATGAA 6 cut(s) 18, 418, 672, 692, 848, 904
TspGWI ACGGA 2 cut(s) 870, 1014
TspRI CASTG 4 cut(s) 399, 429, 781, 1325
VpaK11BI GGWCC 2 cut(s) 938, 1514
VspI ATTAAT 4 cut(s) 56, 189, 387, 1053
XagI CCTNNNNNAGG 1 cut(s) 583
XapI RAATTY 2 cut(s) 722, 987
XceI RCATGY 1 cut(s) 1240
XhoI CTCGAG 1 cut(s) 549
XmiI GTMKAC 3 cut(s) 627, 849, 1135
XmnI GAANNNNTTC 1 cut(s) 611
XspI CTAG 3 cut(s) 596, 1115, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.