pycom05g00190

glycosyltransferase At5g03795

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000727
Physical Location & Seq
Reverse (-)
229771 .. 230142
372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00190.1

Sequence Viewer

Length: 372 bp
ATGCTGAAGAAGATCAGGTTCCGCTTGTGCCCTAGTGGTTACGAAGTGGCAAGCCCGAGAGTGGTGGAAGCCATATACGCAGAGTGTGTTCCAGTGTTGATTTCGGACAGCTATGTTCCGCCGTTCAGCGATGTTCTGGAGTGGAAGTCGTTTTCTGCGCAAGTGCAAGTGAAGGACATACCAAACATCAAAAGGATACTGATGGGAATATCACAAAGTCAGTACCTGAGAATGCAGAGGAGGGTGAAACAGGTGCAGAGGCATTTTGTGGTGAACGGACCTTCCAAGAGATTCGATGTTTTCCATATGATTGTTCACTCCATTTGGCTCAGGAGGTTGAACATCCGCATTGAGGACGATCCAGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

124

Amino Acids

14.47

Weight (kDa)

9.79

Isoelectric Point (pI)

65.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 2 - 68 3e-21 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 159
AciI CCGC 3 cut(s) 22, 119, 346
AclWI GGATC 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 61, 352
AgsI TTSAA 1 cut(s) 340
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 1 cut(s) 111
AluI AGCT 1 cut(s) 111
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 226
Ama87I CYCGRG 1 cut(s) 55
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 256, 283
AvaI CYCGRG 1 cut(s) 55
AvaII GGWCC 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 32
BccI CCATC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 106
BciVI GTATCC 1 cut(s) 189
BfaI CTAG 1 cut(s) 33
BfuI GTATCC 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 55
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 158
Bpu10I CCTNAGC 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 352
Bse1I ACTGG 2 cut(s) 92, 362
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 61, 352
BseMII CTCAG 2 cut(s) 218, 343
BseNI ACTGG 2 cut(s) 92, 362
BseRI GAGGAG 1 cut(s) 253
BseSI GKGCMC 1 cut(s) 32
BsgI GTGCAG 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 55
BslI CCNNNNNNNGG 2 cut(s) 61, 352
BsmI GAATGC 1 cut(s) 237
BsoBI CYCGRG 1 cut(s) 55
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 12, 358
BspACI CCGC 3 cut(s) 22, 119, 346
BspCNI CTCAG 2 cut(s) 219, 342
BspLI GGNNCC 1 cut(s) 20
BspPI GGATC 1 cut(s) 353
BsrI ACTGG 2 cut(s) 92, 362
BssMI GATC 2 cut(s) 12, 358
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 2 cut(s) 227, 329
BstF5I GGATG 1 cut(s) 342
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 2 cut(s) 15, 361
BstMBI GATC 2 cut(s) 12, 358
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstSLI GKGCMC 1 cut(s) 32
BsuI GTATCC 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 144
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 52
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 223
CviJI RGCY 4 cut(s) 54, 71, 111, 328
CviKI_1 RGCY 4 cut(s) 54, 71, 111, 328
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 227, 329
DpnI GATC 2 cut(s) 14, 360
DpnII GATC 2 cut(s) 12, 358
EciI GGCGGA 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 55
FaiI YATR 6 cut(s) 74, 76, 114, 179, 306, 308
FauNDI CATATG 1 cut(s) 306
FokI GGATG 1 cut(s) 329
FspBI CTAG 1 cut(s) 33
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 1 cut(s) 159
GsuI CTGGAG 1 cut(s) 158
HhaI GCGC 1 cut(s) 160
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HinfI GANTC 1 cut(s) 291
HphI GGTGA 2 cut(s) 256, 283
Hpy166II GTNNAC 2 cut(s) 274, 316
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 2 cut(s) 137, 331
Hpy8I GTNNAC 2 cut(s) 274, 316
HpyAV CCTTC 2 cut(s) 166, 291
HpyCH4V TGCA 3 cut(s) 166, 235, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 2 cut(s) 227, 329
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 2 cut(s) 12, 358
LpnPI CCDG 5 cut(s) 105, 122, 236, 239, 316
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 38
MalI GATC 2 cut(s) 14, 360
MboI GATC 2 cut(s) 12, 358
MboII GAAGA 2 cut(s) 19, 22
MhlI GDGCHC 1 cut(s) 32
MnlI CCTC 5 cut(s) 231, 234, 252, 327, 346
Mva1269I GAATGC 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeI CATATG 1 cut(s) 306
NdeII GATC 2 cut(s) 12, 358
NlaIV GGNNCC 1 cut(s) 20
NsbI TGCGCA 1 cut(s) 159
PctI GAATGC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 20
PspPI GGNCC 1 cut(s) 278
PstNI CAGNNNCTG 1 cut(s) 226
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
Sau3AI GATC 2 cut(s) 12, 358
Sau96I GGNCC 1 cut(s) 278
SduI GDGCHC 1 cut(s) 32
SetI ASST 6 cut(s) 20, 113, 228, 255, 283, 338
SinI GGWCC 1 cut(s) 278
SsiI CCGC 3 cut(s) 22, 119, 346
SspMI CTAG 1 cut(s) 33
TaqI TCGA 1 cut(s) 294
TfiI GAWTC 1 cut(s) 291
TscAI CASTG 1 cut(s) 99
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 278
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.