RchiOBHm_Chr3g0471641

Glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000727
Physical Location & Seq
Forward (+)
17486741 .. 17489579
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43738

Sequence Viewer

Length: 1647 bp
ATGATGGCGTATAGTAACAAGAAACCGGGCGGCGGCGGCGGTGGTGCTGGTAGTACTGGTTCATATGCATGTTGGTCTTATTCGTCTTACTCAGCAATTTTGGTGCTTATTGTTCCATTGGTTGTGGTTTTCGGGTTGATTTCGCTGTTGAGTCCGAATTCCTCCAATTGGGTTTTCATATCCAACTACTACTTAACCGGCCCTTCTTCGAATACGAGTACCACAAACTCGTCGGAGATCGGAGCTTCACACGACAACATTTTGGGCTTGAGGTCGACGGTGGTTGTTATGGATATGCACAGTAGTACTATAGAGGAAGTTCACTCAGATGATTATGTACAGAATCGGTCTGCTTCTCCGCCGCTCGCCATAGAAGAAGCGACACCACCACCACTTCAACAACCGATTATTAATGGGACAAAGGACGAAGACCCCATGAATGAAACTCATGCTTCTATCATGAATAATCCTGCTACAAGACAAAAGGAGTATACTAGTTTAGGGCGGCTAGAGGGTCGTCTTCGAAGAGCTCGAGTGGCCATAAGAGAGGCTCGATTTGGAAATAAAACACATGATGCAGATTACATTCCTAATGGTCCAATGTATTGGAATGCTAATGCCTTTCACAGGAGCTACTTGGAAATGGAGAAACGGTTTAAAGTCTTTGTATATGAAGAAGGGGAACCGCCACTGTTTCATAATGGCCCTTGCAAAAGTATATACTCTATGGAGGGAAATTTTATACATGAGATTGAGGTGAATAACCAATTTCGGACTCGAAATCCTGAGGAAGCGCATGTTTATTTTCTTCCCTACAGTGTGACAATGTTGGTTCGGTTCGTATATGTGCGTGACTCGCATGATTTCAGTCCCATCAGACAGACAGTAAGGGATTATGTCAATATTATCTCCACCAAATATCCCTATTGGAATAGAAGCCTTGCAGCTGATCACTTTACACTTGCTTGCCATGATTGGGGGCCAGAGACTTCAAATTCAGATCCTTATCTGCGCAAAAATTCAATCCGTGGTCTATGCAATGCAAATACCTCCGAAGGGTTTAACCCCTCTAAGGATGTCTCCTTTCCAGAAATCAATCTTCAAACTGGTGACACACACGGCTTCCTTGGCGGGCCTTCTCCAAGGCTGCGCTCGATATTAGCTTTCTTTGCTGGAGGAATTCACGGTCCTATTAGGCCTATATTGCTAGAGCATTGGGAAAACAAAGATGAGGAATTGAAGGTGCATCAGTATCTTCCAAAGGGTGTCAACTATTATGACATGATGAGAAAGAGTAAATTCTGTCTTTGCCCAAGCGGGTACGAAGTTGCAAGCCCGAGGGTGGTTGAGGCAATATACACAGGGTGTGTTCCTGTCCTAATTTCAGATCATTATGTGCCACCTTTCGGTGATGTTCTGAATTGGAAGTCTTTCTCCATTGAGGTTTCAGTGAGTGATATTCCTAACTTGAAGAACATATTGACGAGCATATCTACGAGACAATATCTAAGAATGCAAAGGAGAGTGATACAAGTCAGGAGACATTTTGAGGTCAATTCTCCTCCTAAACGATTTGATGTTTTCCATATGATCCTACATTCTATTTGGCTAAGAAGACTCAATGTTAGAGTACATGATGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

548

Amino Acids

62.21

Weight (kDa)

7.34

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 215 - 497 1.9e-58 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1013
AccBSI CCGCTC 1 cut(s) 364
AccI GTMKAC 2 cut(s) 275, 491
AclWI GGATC 2 cut(s) 995, 1585
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 6 cut(s) 157, 736, 994, 1018, 1179, 1298
AdeI CACNNNGTG 1 cut(s) 1365
AfaI GTAC 6 cut(s) 55, 220, 307, 339, 1322, 1632
AfiI CCNNNNNNNGG 8 cut(s) 32, 168, 627, 976, 1056, 1072, 1342, 1406
AgsI TTSAA 6 cut(s) 398, 993, 1023, 1103, 1240, 1471
AhlI ACTAGT 1 cut(s) 494
AjuI GAANNNNNNNTTGG 2 cut(s) 158, 190
AluBI AGCT 5 cut(s) 245, 530, 633, 947, 1163
AluI AGCT 5 cut(s) 245, 530, 633, 947, 1163
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 4 cut(s) 980, 1084, 1492, 1534
AlwI GGATC 2 cut(s) 995, 1585
Ama87I CYCGRG 2 cut(s) 531, 1336
AoxI GGCC 6 cut(s) 199, 537, 703, 980, 1133, 1196
ApeKI GCWGC 2 cut(s) 944, 1147
ApoI RAATTY 6 cut(s) 157, 736, 994, 1018, 1179, 1298
AseI ATTAAT 1 cut(s) 411
Asp700I GAANNNNTTC 1 cut(s) 1430
AspLEI GCGC 3 cut(s) 796, 1014, 1152
AspS9I GGNCC 6 cut(s) 200, 596, 704, 980, 1133, 1187
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 3 cut(s) 769, 1121, 1421
AsuII TTCGAA 2 cut(s) 209, 523
AvaI CYCGRG 2 cut(s) 531, 1336
AvaII GGWCC 2 cut(s) 596, 1187
AxyI CCTNAGG 1 cut(s) 786
BalI TGGCCA 1 cut(s) 539
BanII GRGCYC 1 cut(s) 532
BbsI GAAGAC 3 cut(s) 435, 512, 1621
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 2 cut(s) 956, 1134
BccI CCATC 1 cut(s) 881
BceAI ACGGC 1 cut(s) 1135
BclI TGATCA 2 cut(s) 949, 1639
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 4 cut(s) 980, 1084, 1492, 1534
BcuI ACTAGT 1 cut(s) 494
BfaI CTAG 3 cut(s) 495, 509, 1208
BfmI CTRYAG 2 cut(s) 309, 814
BisI GCNGC 7 cut(s) 31, 34, 37, 362, 506, 945, 1148
BlsI GCNGC 7 cut(s) 32, 35, 38, 363, 507, 946, 1149
BmcAI AGTACT 2 cut(s) 55, 307
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 596, 1187
BmeT110I CYCGRG 2 cut(s) 531, 1336
BmgT120I GGNCC 6 cut(s) 200, 596, 704, 980, 1133, 1187
BmiI GGNNCC 2 cut(s) 684, 981
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 565, 1255
BpiI GAAGAC 3 cut(s) 435, 512, 1621
BpmI CTGGAG 1 cut(s) 1194
Bpu14I TTCGAA 2 cut(s) 209, 523
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 4 cut(s) 1027, 1126, 1142, 1337
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 168, 627, 976, 1056, 1072, 1342, 1406
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 61, 1111
Bse21I CCTNAGG 1 cut(s) 786
Bse3DI GCAATG 1 cut(s) 1045
Bse8I GATNNNNATC 1 cut(s) 1005
BseDI CCNNGG 4 cut(s) 1027, 1126, 1142, 1337
BseGI GGATG 1 cut(s) 1081
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 8 cut(s) 32, 168, 627, 976, 1056, 1072, 1342, 1406
BseMI GCAATG 1 cut(s) 1045
BseMII CTCAG 3 cut(s) 105, 339, 777
BseNI ACTGG 2 cut(s) 61, 1111
BseRI GAGGAG 1 cut(s) 1551
BseXI GCAGC 2 cut(s) 956, 1134
BshFI GGCC 6 cut(s) 201, 539, 705, 982, 1135, 1198
BsiHKAI GWGCWC 1 cut(s) 532
BsiHKCI CYCGRG 2 cut(s) 531, 1336
BsiSI CCGG 2 cut(s) 26, 198
BslFI GGGAC 2 cut(s) 430, 855
BslI CCNNNNNNNGG 8 cut(s) 32, 168, 627, 976, 1056, 1072, 1342, 1406
BsmAI GTCTC 4 cut(s) 980, 1084, 1492, 1534
BsmFI GGGAC 2 cut(s) 430, 855
BsmI GAATGC 2 cut(s) 616, 1518
BsnI GGCC 6 cut(s) 201, 539, 705, 982, 1135, 1198
BsoBI CYCGRG 2 cut(s) 531, 1336
Bsp119I TTCGAA 2 cut(s) 209, 523
Bsp1286I GDGCHC 1 cut(s) 532
Bsp1407I TGTACA 1 cut(s) 337
Bsp143I GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
BspANI GGCC 6 cut(s) 201, 539, 705, 982, 1135, 1198
BspCNI CTCAG 3 cut(s) 104, 338, 778
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 684, 981
BspPI GGATC 2 cut(s) 995, 1585
BspQI GCTCTTC 1 cut(s) 520
BspT104I TTCGAA 2 cut(s) 209, 523
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 1045
BsrFI RCCGGY 1 cut(s) 197
BsrGI TGTACA 1 cut(s) 337
BsrI ACTGG 2 cut(s) 61, 1111
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 4 cut(s) 1027, 1126, 1142, 1337
BssMI GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
BssNAI GTATAC 1 cut(s) 492
BssT1I CCWWGG 2 cut(s) 1126, 1142
Bst1107I GTATAC 1 cut(s) 492
Bst4CI ACNGT 7 cut(s) 280, 302, 654, 693, 818, 886, 1187
Bst6I CTCTTC 1 cut(s) 520
BstAUI TGTACA 1 cut(s) 337
BstBI TTCGAA 2 cut(s) 209, 523
BstC8I GCNNGC 4 cut(s) 366, 967, 1133, 1333
BstDEI CTNAG 6 cut(s) 91, 325, 786, 1071, 1508, 1610
BstDSI CCRYGG 1 cut(s) 1027
BstENI CCTNNNNNAGG 1 cut(s) 625
BstF5I GGATG 1 cut(s) 1081
BstHHI GCGC 3 cut(s) 796, 1014, 1152
BstKTI GATC 6 cut(s) 240, 952, 1003, 1390, 1593, 1642
BstMAI GTCTC 4 cut(s) 980, 1084, 1492, 1534
BstMBI GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
BstMWI GCNNNNNNNGC 6 cut(s) 36, 536, 856, 1128, 1169, 1204
BstNSI RCATGY 2 cut(s) 72, 800
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 2 cut(s) 309, 814
BstV1I GCAGC 2 cut(s) 956, 1134
BstV2I GAAGAC 3 cut(s) 435, 512, 1621
BstX2I RGATCY 1 cut(s) 1000
BstXI CCANNNNNNTGG 1 cut(s) 606
BstYI RGATCY 1 cut(s) 1000
BstZ17I GTATAC 1 cut(s) 492
Bsu36I CCTNAGG 1 cut(s) 786
BsuRI GGCC 6 cut(s) 201, 539, 705, 982, 1135, 1198
BtgI CCRYGG 1 cut(s) 1027
BtsCI GGATG 1 cut(s) 1081
BtsIMutI CAGTG 3 cut(s) 689, 823, 1455
Cac8I GCNNGC 4 cut(s) 366, 967, 1133, 1333
CciI TCATGA 1 cut(s) 459
CfoI GCGC 3 cut(s) 796, 1014, 1152
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 6 cut(s) 200, 596, 704, 980, 1133, 1187
Csp6I GTAC 6 cut(s) 54, 219, 306, 338, 1321, 1631
CviQI GTAC 6 cut(s) 54, 219, 306, 338, 1321, 1631
DdeI CTNAG 6 cut(s) 91, 325, 786, 1071, 1508, 1610
DpnI GATC 6 cut(s) 239, 951, 1002, 1389, 1592, 1641
DpnII GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
DraI TTTAAA 1 cut(s) 658
DraIII CACNNNGTG 1 cut(s) 1365
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
EciI GGCGGA 1 cut(s) 348
Ecl136II GAGCTC 1 cut(s) 530
Eco130I CCWWGG 2 cut(s) 1126, 1142
Eco147I AGGCCT 1 cut(s) 1198
Eco24I GRGCYC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 596, 1187
Eco53kI GAGCTC 1 cut(s) 530
Eco81I CCTNAGG 1 cut(s) 786
Eco88I CYCGRG 2 cut(s) 531, 1336
EcoICRI GAGCTC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 625
EcoRI GAATTC 2 cut(s) 157, 1179
EcoT14I CCWWGG 2 cut(s) 1126, 1142
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 532
ErhI CCWWGG 2 cut(s) 1126, 1142
FaqI GGGAC 2 cut(s) 430, 855
FauI CCCGC 2 cut(s) 1124, 1310
FauNDI CATATG 2 cut(s) 64, 1587
FbaI TGATCA 2 cut(s) 949, 1639
FblI GTMKAC 2 cut(s) 275, 491
Fnu4HI GCNGC 7 cut(s) 31, 34, 37, 362, 506, 945, 1148
FokI GGATG 1 cut(s) 1088
FriOI GRGCYC 1 cut(s) 532
Fsp4HI GCNGC 7 cut(s) 31, 34, 37, 362, 506, 945, 1148
FspBI CTAG 3 cut(s) 495, 509, 1208
FspI TGCGCA 1 cut(s) 1013
GlaI GCGC 3 cut(s) 795, 1013, 1151
GluI GCNGC 7 cut(s) 31, 34, 37, 362, 506, 945, 1148
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 6 cut(s) 201, 539, 705, 982, 1135, 1198
HapII CCGG 2 cut(s) 26, 198
HhaI GCGC 3 cut(s) 796, 1014, 1152
Hin6I GCGC 3 cut(s) 794, 1012, 1150
HinP1I GCGC 3 cut(s) 794, 1012, 1150
HincII GTYRAC 2 cut(s) 276, 1270
HindII GTYRAC 2 cut(s) 276, 1270
HinfI GANTC 5 cut(s) 151, 343, 775, 854, 1617
HpaII CCGG 2 cut(s) 26, 198
HphI GGTGA 3 cut(s) 769, 1121, 1421
Hpy166II GTNNAC 4 cut(s) 276, 322, 492, 1270
Hpy188III TCNNGA 4 cut(s) 460, 785, 1088, 1537
Hpy8I GTNNAC 4 cut(s) 276, 322, 492, 1270
Hpy99I CGWCG 2 cut(s) 235, 280
HpyAV CCTTC 5 cut(s) 213, 671, 1049, 1146, 1234
HpyCH4III ACNGT 7 cut(s) 280, 302, 654, 693, 818, 886, 1187
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 536, 856, 1128, 1169, 1204
HpyF3I CTNAG 6 cut(s) 91, 325, 786, 1071, 1508, 1610
HspAI GCGC 3 cut(s) 794, 1012, 1150
Ksp22I TGATCA 2 cut(s) 949, 1639
Kzo9I GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
LguI GCTCTTC 1 cut(s) 520
LmnI GCTCC 2 cut(s) 242, 630
Lsp1109I GCAGC 2 cut(s) 956, 1134
LweI GCATC 2 cut(s) 565, 1255
MaeI CTAG 3 cut(s) 495, 509, 1208
MaeIII GTNAC 4 cut(s) 14, 820, 851, 1109
MalI GATC 6 cut(s) 239, 951, 1002, 1389, 1592, 1641
MbiI CCGCTC 1 cut(s) 364
MboI GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
MfeI CAATTG 1 cut(s) 166
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 1 cut(s) 532
MlsI TGGCCA 1 cut(s) 539
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 4 cut(s) 160, 769, 848, 1611
MmeI TCCRAC 2 cut(s) 207, 213
Mox20I TGGCCA 1 cut(s) 539
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 1430
MscI TGGCCA 1 cut(s) 539
MseI TTAA 4 cut(s) 194, 411, 657, 1062
MslI CAYNNNNRTG 2 cut(s) 67, 327
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 2 cut(s) 26, 198
MspR9I CCNGG 1 cut(s) 27
MunI CAATTG 1 cut(s) 166
Mva1269I GAATGC 2 cut(s) 616, 1518
MwoI GCNNNNNNNGC 6 cut(s) 36, 536, 856, 1128, 1169, 1204
NciI CCSGG 1 cut(s) 27
NdeI CATATG 2 cut(s) 64, 1587
NdeII GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
NlaIV GGNNCC 2 cut(s) 684, 981
NmuCI GTSAC 3 cut(s) 820, 851, 1109
NsbI TGCGCA 1 cut(s) 1013
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 2 cut(s) 72, 800
NspV TTCGAA 2 cut(s) 209, 523
PaeR7I CTCGAG 1 cut(s) 531
PagI TCATGA 1 cut(s) 459
PceI AGGCCT 1 cut(s) 1198
PciSI GCTCTTC 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 529
PctI GAATGC 2 cut(s) 616, 1518
PdmI GAANNNNTTC 1 cut(s) 1430
PfeI GAWTC 1 cut(s) 343
PkrI GCNGC 7 cut(s) 32, 35, 38, 363, 507, 946, 1149
PleI GAGTC 4 cut(s) 159, 769, 848, 1611
PpsI GAGTC 4 cut(s) 159, 769, 848, 1611
PshBI ATTAAT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 684, 981
PspPI GGNCC 6 cut(s) 200, 596, 704, 980, 1133, 1187
PspXI VCTCGAGB 1 cut(s) 531
PsrI GAACNNNNNNTAC 2 cut(s) 43, 75
PsuI RGATCY 1 cut(s) 1000
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 6 cut(s) 55, 220, 307, 339, 1322, 1632
RsaNI GTAC 6 cut(s) 54, 219, 306, 338, 1321, 1631
RseI CAYNNNNRTG 2 cut(s) 67, 327
SacI GAGCTC 1 cut(s) 532
SalI GTCGAC 1 cut(s) 274
SapI GCTCTTC 1 cut(s) 520
SaqAI TTAA 4 cut(s) 194, 411, 657, 1062
SatI GCNGC 7 cut(s) 31, 34, 37, 362, 506, 945, 1148
Sau3AI GATC 6 cut(s) 237, 949, 1000, 1387, 1590, 1639
Sau96I GGNCC 6 cut(s) 200, 596, 704, 980, 1133, 1187
ScaI AGTACT 2 cut(s) 55, 307
SchI GAGTC 4 cut(s) 160, 769, 848, 1611
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 532
SfaNI GCATC 2 cut(s) 565, 1255
SfcI CTRYAG 2 cut(s) 309, 814
Sfr274I CTCGAG 1 cut(s) 531
SfuI TTCGAA 2 cut(s) 209, 523
SinI GGWCC 2 cut(s) 596, 1187
SlaI CTCGAG 1 cut(s) 531
SmiMI CAYNNNNRTG 2 cut(s) 67, 327
SmlI CTYRAG 2 cut(s) 268, 531
SmoI CTYRAG 2 cut(s) 268, 531
SpeI ACTAGT 1 cut(s) 494
SseBI AGGCCT 1 cut(s) 1198
SspI AATATT 1 cut(s) 904
SspMI CTAG 3 cut(s) 495, 509, 1208
SstI GAGCTC 1 cut(s) 532
StuI AGGCCT 1 cut(s) 1198
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 2 cut(s) 1126, 1142
TaaI ACNGT 7 cut(s) 280, 302, 654, 693, 818, 886, 1187
TaqI TCGA 7 cut(s) 209, 275, 523, 532, 553, 778, 1154
TaqII GACCGA 1 cut(s) 336
TatI WGTACW 4 cut(s) 53, 305, 337, 1630
TauI GCSGC 5 cut(s) 33, 36, 39, 364, 508
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 4 cut(s) 194, 411, 657, 1062
Tru9I TTAA 4 cut(s) 194, 411, 657, 1062
TscAI CASTG 3 cut(s) 696, 823, 1455
TseFI GTSAC 3 cut(s) 820, 851, 1109
TseI GCWGC 2 cut(s) 944, 1147
Tsp45I GTSAC 3 cut(s) 820, 851, 1109
TspDTI ATGAA 7 cut(s) 51, 166, 452, 456, 476, 686, 687
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 3 cut(s) 696, 823, 1455
VpaK11BI GGWCC 2 cut(s) 596, 1187
VspI ATTAAT 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 625
XapI RAATTY 6 cut(s) 157, 736, 994, 1018, 1179, 1298
XceI RCATGY 2 cut(s) 72, 800
XhoI CTCGAG 1 cut(s) 531
XmiI GTMKAC 2 cut(s) 275, 491
XmnI GAANNNNTTC 1 cut(s) 1430
XspI CTAG 3 cut(s) 495, 509, 1208
ZrmI AGTACT 2 cut(s) 55, 307
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.