MD08G1186100.v1.1

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000422
Physical Location & Seq
Forward (+)
23441309 .. 23442244
936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1186100.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGCAGCTTTTGCAATTTTGAAACTAGCCCTTGTTATTTCTTTGTTTCATTTCAGCTCAGCAGCTAGGAACCTTGCTGAAACTTCCAACCAAAACCAAATGCAATTCCAGTACCACAACGGCCCTCTTCTCATCGGAACATTCTCCGTCAACTTGATCTGGTACGGCCGCTTTACCCCTTCCCAGCGTGCAATCGTTTCCGATTTTATCTCCTCTCTATCCAAACCCACCTCCGACCAACCATCCGTCGCCACATGGTGGAAGTCCATAGAGAAATACTACCACCTCTCGAAAAAACCCTCTTCATCTCTCTCCCTCTCTCTAGGAACCCAAATTCTTGATGAAAATTACTCTCTGGGAAAATCTCTCACCTCCCGCCAGATCCAACAGCTGGCCTCAAAGGGCACCCAAAACTACGGGATCAATGTCGTTTTGACATCGTCTGATGTTCTTGTTGATGGGTTTTGCATGAACCAGTGCGGGACCCACGGCTCATCCAACAGCGGTAGTCAAATCAGAGGATCAAAAACCAAGTTTACTTACATTTGGGTTGGAAACTCAGAGACCCAATGCCCAGGGCAATGCGCGTGGCCGTTCCACCAGCCCATCTATGGACCCCAGAGCCCACCTCTCGTGGCCCCCAACAACGACGTGGGCCTGGATGGCATTGTGATCAACCTCGCTAGTCTTTTGGCAGGGACCGTGACCAACCCCTTCGGAAATGGCTACTTCCAGGGTCCGAAGGAGGCTCCTTTGGAAGCTGCTTCCGCTTGCCCCGGGGTCTACGGCAAGGGGTCCTACCCTGGTTACGCTGGGGACCTACTAGTGGACCCCACAACCGGAGCTAGCTACAATGCTCATGGATCTAATGGGAAGAAGTACTTGCTTCCTGCTTTGTACGACCCTTCGACTTCAACTTGTTCTACTTTGGTTTAG

Protein Analysis

312

Amino Acids

33.32

Weight (kDa)

8.44

Isoelectric Point (pI)

23.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 37 - 310 7.6e-124 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35140 AT4G08950
fragaria_vesca FvH4_5g36900 FvH4_5g36910 FvH4_5g36920 FvH4_5g36930
malus_domestica MD08G1185900.v1.1 MD08G1186000.v1.1 MD08G1186100.v1.1 MD08G1186300.v1.1 MD15G1372200.v1.1 MD15G1372400.v1.1
prunus_persica Prupe.1G520600_v2.0.a1 Prupe.1G520700_v2.0.a1 Prupe.1G520800_v2.0.a1
pyrus_communis pycom08g15950 pycom08g15960 pycom08g15970 pycom08g16010 pycom15g33320 pycom15g33330
rosa_chinensis RchiOBHm_Chr0c28g0500911 RchiOBHm_Chr0c28g0500921 RchiOBHm_Chr0c28g0500931 RchiOBHm_Chr0c28g0500981 RchiOBHm_Chr0c28g0501021 RchiOBHm_Chr7g0243461 RchiOBHm_Chr7g0243471 RchiOBHm_Chr7g0243481 RchiOBHm_Chr7g0243521 RchiOBHm_Chr7g0243531 RchiOBHm_Chr7g0243541 RchiOBHm_Chr7g0243581 RchiOBHm_Chr7g0243621 RchiOBHm_Chr7g0243641 RchiOBHm_Chr7g0243671 RchiOBHm_Chr7g0243681 RchiOBHm_Chr7g0243691
rosa_laevigata RLG00000000840 RLG00000000841 RLG00000000842 RLG00000000843 RLG00000000844 RLG00000000845
rosa_multiflora Rmu_sc0000477.1_g000001 Rmu_sc0000477.1_g000003 Rmu_sc0001036.1_g000001 Rmu_sc0001036.1_g000002 Rmu_sc0004062.1_g000001 Rmu_sc0007755.1_g000001 Rmu_sc0008223.1_g000001 Rmu_sc0008473.1_g000006 Rmu_sc0009153.1_g000001 Rmu_sc0009153.1_g000002 Rmu_sc0029916.1_g000001
rosa_roxburghii Rroxscaffold_3G00222020 Rroxscaffold_3G00222030 Rroxscaffold_3G00222040 Rroxscaffold_3G00222060 Rroxscaffold_3G00222100
rosa_rugosa Rorug07G0319800.1 Rorug07G0319900 Rorug07G0320000 Rorug07G0320000 Rorug07G0320100 Rorug07G0320200 Rorug07G0320300
rosa_samantha Rh7BG445800 Rh7BG445900 Rh7BG446000 Rh7BG446100 Rh7BG446200 Rh7BG446300 Rh7BG446400 Rh7BG446500 Rh7CG491600 Rh7CG491700 Rh7CG491800 Rh7CG492000 Rh7CG492100 Rh7CG492200 Rh7CG492300 Rh7CG492500 Rh7CG492600 Rh7DG459900 Rh7DG460000 Rh7DG460100 Rh7DG460200 Rh7DG460300 Rh7DG460400 Rh7DG460600 Rh7DG460700
rosa_wichuraiana Rw7G039570 Rw7G039580 Rw7G039590 Rw7G039620 Rw7G039630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 2 cut(s) 258, 391
AccI GTMKAC 1 cut(s) 783
AccII CGCG 1 cut(s) 587
AciI CCGC 5 cut(s) 169, 376, 480, 504, 768
AclWI GGATC 4 cut(s) 376, 428, 529, 871
AcoI YGGCCR 2 cut(s) 166, 590
AcsI RAATTY 1 cut(s) 333
AdeI CACNNNGTG 1 cut(s) 258
AfaI GTAC 4 cut(s) 113, 164, 881, 899
AfiI CCNNNNNNNGG 5 cut(s) 258, 391, 611, 826, 839
AgsI TTSAA 2 cut(s) 22, 915
AhlI ACTAGT 1 cut(s) 823
AjiI CACGTC 1 cut(s) 652
AjnI CCWGG 4 cut(s) 574, 657, 732, 802
AluBI AGCT 7 cut(s) 8, 57, 65, 391, 761, 845, 849
AluI AGCT 7 cut(s) 8, 57, 65, 391, 761, 845, 849
Alw26I GTCTC 1 cut(s) 557
AlwI GGATC 4 cut(s) 376, 428, 529, 871
Ama87I CYCGRG 1 cut(s) 776
AoxI GGCC 6 cut(s) 121, 166, 393, 590, 636, 655
ApeKI GCWGC 3 cut(s) 5, 62, 761
ApoI RAATTY 1 cut(s) 333
AspLEI GCGC 1 cut(s) 587
AsuC2I CCSGG 2 cut(s) 777, 778
AsuHPI GGTGA 1 cut(s) 361
AsuNHI GCTAGC 1 cut(s) 845
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 7 cut(s) 483, 614, 699, 737, 795, 817, 829
BaeGI GKGCMC 1 cut(s) 407
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 626
BauI CACGAG 1 cut(s) 632
BbvI GCAGC 3 cut(s) 17, 74, 748
BccI CCATC 4 cut(s) 250, 452, 614, 656
BceAI ACGGC 5 cut(s) 136, 181, 505, 577, 802
BciT130I CCWGG 4 cut(s) 576, 659, 734, 804
BclI TGATCA 1 cut(s) 672
BcnI CCSGG 2 cut(s) 777, 778
BcoDI GTCTC 1 cut(s) 557
BcuI ACTAGT 1 cut(s) 823
BfaI CTAG 6 cut(s) 26, 66, 323, 684, 824, 846
BglI GCCNNNNNGGC 1 cut(s) 663
BisI GCNGC 4 cut(s) 6, 63, 169, 762
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 4 cut(s) 7, 64, 170, 763
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 6 cut(s) 576, 659, 734, 777, 778, 804
Bme18I GGWCC 7 cut(s) 483, 614, 699, 737, 795, 817, 829
BmeT110I CYCGRG 1 cut(s) 776
BmgBI CACGTC 1 cut(s) 652
BmrFI CCNGG 6 cut(s) 576, 659, 734, 777, 778, 804
BmtI GCTAGC 1 cut(s) 849
BplI GAGNNNNNCTC 2 cut(s) 613, 645
Bpu1102I GCTNAGC 1 cut(s) 58
BpuMI CCSGG 2 cut(s) 777, 778
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 8 cut(s) 487, 574, 575, 733, 775, 776, 777, 802
BsaWI WCCGGW 1 cut(s) 839
Bsc4I CCNNNNNNNGG 5 cut(s) 258, 391, 611, 826, 839
Bse1I ACTGG 2 cut(s) 109, 475
Bse3DI GCAATG 1 cut(s) 587
BseBI CCWGG 4 cut(s) 576, 659, 734, 804
BseDI CCNNGG 8 cut(s) 487, 574, 575, 733, 775, 776, 777, 802
BseGI GGATG 3 cut(s) 242, 494, 667
BseLI CCNNNNNNNGG 5 cut(s) 258, 391, 611, 826, 839
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 2 cut(s) 72, 573
BseNI ACTGG 2 cut(s) 109, 475
BseRI GAGGAG 1 cut(s) 202
BseSI GKGCMC 1 cut(s) 407
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 17, 74, 748
BseYI CCCAGC 2 cut(s) 183, 812
Bsh1236I CGCG 1 cut(s) 587
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 6 cut(s) 123, 168, 395, 592, 638, 657
BshNI GGYRCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 2 cut(s) 777, 840
BslFI GGGAC 3 cut(s) 496, 712, 830
BslI CCNNNNNNNGG 5 cut(s) 258, 391, 611, 826, 839
BsmAI GTCTC 1 cut(s) 557
BsmFI GGGAC 3 cut(s) 496, 712, 830
BsnI GGCC 6 cut(s) 123, 168, 395, 592, 638, 657
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 776
Bsp1286I GDGCHC 2 cut(s) 407, 626
Bsp143I GATC 6 cut(s) 156, 381, 420, 521, 672, 863
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 5 cut(s) 169, 376, 480, 504, 768
BspANI GGCC 6 cut(s) 123, 168, 395, 592, 638, 657
BspCNI CTCAG 2 cut(s) 71, 572
BspFNI CGCG 1 cut(s) 587
BspOI GCTAGC 1 cut(s) 849
BspPI GGATC 4 cut(s) 376, 428, 529, 871
BspT107I GGYRCC 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 587
BsrI ACTGG 2 cut(s) 109, 475
BssECI CCNNGG 8 cut(s) 487, 574, 575, 733, 775, 776, 777, 802
BssMI GATC 6 cut(s) 156, 381, 420, 521, 672, 863
BssSI CACGAG 1 cut(s) 632
Bst2BI CACGAG 1 cut(s) 632
Bst2UI CCWGG 4 cut(s) 576, 659, 734, 804
Bst4CI ACNGT 1 cut(s) 703
Bst6I CTCTTC 2 cut(s) 132, 307
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 189, 393, 772, 847
BstDEI CTNAG 2 cut(s) 58, 559
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 3 cut(s) 242, 494, 667
BstFNI CGCG 1 cut(s) 587
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 6 cut(s) 159, 384, 423, 524, 675, 866
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 6 cut(s) 156, 381, 420, 521, 672, 863
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 11, 663, 767
BstNI CCWGG 4 cut(s) 576, 659, 734, 804
BstSCI CCNGG 6 cut(s) 574, 657, 732, 775, 776, 802
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 3 cut(s) 17, 74, 748
BstX2I RGATCY 2 cut(s) 381, 863
BstYI RGATCY 2 cut(s) 381, 863
BstZI CGGCCG 1 cut(s) 166
BsuRI GGCC 6 cut(s) 123, 168, 395, 592, 638, 657
BtgI CCRYGG 1 cut(s) 487
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 3 cut(s) 242, 494, 667
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 4 cut(s) 189, 393, 772, 847
CfoI GCGC 1 cut(s) 587
Cfr9I CCCGGG 1 cut(s) 776
Csp6I GTAC 4 cut(s) 112, 163, 880, 898
CspCI CAANNNNNGTGG 2 cut(s) 569, 604
CviAII CATG 3 cut(s) 255, 469, 860
CviQI GTAC 4 cut(s) 112, 163, 880, 898
DdeI CTNAG 2 cut(s) 58, 559
DpnI GATC 6 cut(s) 158, 383, 422, 523, 674, 865
DpnII GATC 6 cut(s) 156, 381, 420, 521, 672, 863
DraIII CACNNNGTG 1 cut(s) 258
EaeI YGGCCR 2 cut(s) 166, 590
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 2 cut(s) 132, 307
EarI CTCTTC 2 cut(s) 132, 307
EclXI CGGCCG 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 626
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 7 cut(s) 483, 614, 699, 737, 795, 817, 829
Eco52I CGGCCG 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 776
EcoO109I RGGNCCY 3 cut(s) 483, 795, 817
EcoRII CCWGG 4 cut(s) 574, 657, 732, 802
EcoT38I GRGCYC 1 cut(s) 626
FaeI CATG 3 cut(s) 258, 472, 863
FaiI YATR 5 cut(s) 256, 269, 470, 612, 861
FalI AAGNNNNNCTT 2 cut(s) 866, 898
FaqI GGGAC 3 cut(s) 496, 712, 830
FatI CATG 3 cut(s) 254, 468, 859
FauI CCCGC 2 cut(s) 383, 473
FbaI TGATCA 1 cut(s) 672
FblI GTMKAC 1 cut(s) 783
Fnu4HI GCNGC 4 cut(s) 6, 63, 169, 762
FokI GGATG 3 cut(s) 229, 481, 674
FriOI GRGCYC 1 cut(s) 626
Fsp4HI GCNGC 4 cut(s) 6, 63, 169, 762
FspBI CTAG 6 cut(s) 26, 66, 323, 684, 824, 846
GlaI GCGC 1 cut(s) 586
GluI GCNGC 4 cut(s) 6, 63, 169, 762
GsaI CCCAGC 2 cut(s) 187, 816
HaeIII GGCC 6 cut(s) 123, 168, 395, 592, 638, 657
HapII CCGG 2 cut(s) 777, 840
HhaI GCGC 1 cut(s) 587
Hin1II CATG 3 cut(s) 258, 472, 863
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HpaII CCGG 2 cut(s) 777, 840
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 4 cut(s) 151, 537, 784, 829
Hpy188I TCNGA 8 cut(s) 137, 202, 235, 445, 518, 562, 719, 741
Hpy188III TCNNGA 2 cut(s) 289, 338
Hpy8I GTNNAC 4 cut(s) 151, 537, 784, 829
Hpy99I CGWCG 2 cut(s) 251, 653
HpyAV CCTTC 4 cut(s) 189, 724, 736, 915
HpyCH4III ACNGT 1 cut(s) 703
HpyCH4IV ACGT 1 cut(s) 651
HpyCH4V TGCA 4 cut(s) 14, 103, 191, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 663, 767
HpyF3I CTNAG 2 cut(s) 58, 559
HpySE526I ACGT 1 cut(s) 651
Hsp92II CATG 3 cut(s) 258, 472, 863
HspAI GCGC 1 cut(s) 585
KflI GGGWCCC 1 cut(s) 483
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 6 cut(s) 156, 381, 420, 521, 672, 863
LmnI GCTCC 2 cut(s) 754, 842
Lsp1109I GCAGC 3 cut(s) 17, 74, 748
MaeI CTAG 6 cut(s) 26, 66, 323, 684, 824, 846
MaeII ACGT 1 cut(s) 651
MaeIII GTNAC 2 cut(s) 703, 806
MalI GATC 6 cut(s) 158, 383, 422, 523, 674, 865
MboI GATC 6 cut(s) 156, 381, 420, 521, 672, 863
MboII GAAGA 3 cut(s) 119, 294, 886
MflI RGATCY 2 cut(s) 381, 863
MhlI GDGCHC 2 cut(s) 407, 626
MluCI AATT 4 cut(s) 15, 104, 333, 346
MmeI TCCRAC 5 cut(s) 111, 258, 409, 522, 532
MspA1I CMGCKG 2 cut(s) 391, 504
MspI CCGG 2 cut(s) 777, 840
MspR9I CCNGG 6 cut(s) 576, 659, 734, 777, 778, 804
MvaI CCWGG 4 cut(s) 576, 659, 734, 804
MvnI CGCG 1 cut(s) 587
MwoI GCNNNNNNNGC 3 cut(s) 11, 663, 767
NciI CCSGG 2 cut(s) 777, 778
NdeII GATC 6 cut(s) 156, 381, 420, 521, 672, 863
NheI GCTAGC 1 cut(s) 845
NlaIII CATG 3 cut(s) 258, 472, 863
NmuCI GTSAC 1 cut(s) 703
PasI CCCWGGG 1 cut(s) 575
PflFI GACNNNGTC 1 cut(s) 439
PflMI CCANNNNNTGG 2 cut(s) 258, 391
PkrI GCNGC 4 cut(s) 7, 64, 170, 763
PpuMI RGGWCCY 3 cut(s) 483, 795, 817
Psp5II RGGWCCY 3 cut(s) 483, 795, 817
Psp6I CCWGG 4 cut(s) 574, 657, 732, 802
PspFI CCCAGC 2 cut(s) 183, 812
PspGI CCWGG 4 cut(s) 574, 657, 732, 802
PspPPI RGGWCCY 3 cut(s) 483, 795, 817
PsuI RGATCY 2 cut(s) 381, 863
PsyI GACNNNGTC 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 4 cut(s) 113, 164, 881, 899
RsaNI GTAC 4 cut(s) 112, 163, 880, 898
SatI GCNGC 4 cut(s) 6, 63, 169, 762
Sau3AI GATC 6 cut(s) 156, 381, 420, 521, 672, 863
ScaI AGTACT 1 cut(s) 881
ScrFI CCNGG 6 cut(s) 576, 659, 734, 777, 778, 804
SduI GDGCHC 2 cut(s) 407, 626
SinI GGWCC 7 cut(s) 483, 614, 699, 737, 795, 817, 829
SmaI CCCGGG 1 cut(s) 778
SpeI ACTAGT 1 cut(s) 823
Sse9I AATT 4 cut(s) 15, 104, 333, 346
SsiI CCGC 5 cut(s) 169, 376, 480, 504, 768
SspMI CTAG 6 cut(s) 26, 66, 323, 684, 824, 846
StyD4I CCNGG 6 cut(s) 574, 657, 732, 775, 776, 802
TaaI ACNGT 1 cut(s) 703
TaiI ACGT 1 cut(s) 654
TaqI TCGA 2 cut(s) 290, 908
TasI AATT 4 cut(s) 15, 104, 333, 346
TatI WGTACW 1 cut(s) 879
TauI GCSGC 1 cut(s) 171
TscAI CASTG 1 cut(s) 482
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 3 cut(s) 5, 62, 761
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 4 cut(s) 38, 294, 357, 485
TspGWI ACGGA 2 cut(s) 136, 235
TspMI CCCGGG 1 cut(s) 776
TspRI CASTG 1 cut(s) 482
Tth111I GACNNNGTC 1 cut(s) 439
Van91I CCANNNNNTGG 2 cut(s) 258, 391
VpaK11BI GGWCC 7 cut(s) 483, 614, 699, 737, 795, 817, 829
XapI RAATTY 1 cut(s) 333
XcmI CCANNNNNNNNNTGG 2 cut(s) 608, 649
XmaI CCCGGG 1 cut(s) 776
XmiI GTMKAC 1 cut(s) 783
XspI CTAG 6 cut(s) 26, 66, 323, 684, 824, 846
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.