Rroxscaffold_3G00222020

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000422
Physical Location & Seq
Forward (+)
5015357 .. 5019402
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222020.1

Sequence Viewer

Length: 1854 bp
ATGGCTTCTTCTTCATCAACCCAGTTGATACTCTCATTCTTCTTAGTGGTCTCACTCTTCCAAGTTTCACTGGCTTCTTCTAGGTTGTTCCAAGTCTCCTTGGCAACAAGGAAGCTCAGTGAGCTTGTTCAAGACCAGACCCAGCTCCTTAAATACCATAACGGTCCTCTTCTCAACGGCAAAATCGCCATCAATCTCATCTGGTACGGCAACTTCAAGCCTGCCCAGAAAGCTATTGTTTCCGATTTCATCACCTCCTTATCTTCTTCCTCACCGGCGCAATCCAACAAGCCCACCGTCGCCACCTGGTGGAAGACCACCGAGAAGTACTACCACCTCACATCCAAGAAGTCTTCTCTTTCCCTTGCTATCGGGAAACAAATTTCCGACGAGAAATACTCGCTCGGAAAGTCCCTCACCAACAAACAGATCGTCAAGTTGGCTTCTCTGGGTGACCAGAAGAACGCAATCAACGTGGTCTTGACTTCAGCCGATGTTGCCGTCGACGGTTTCTGCATGAACCGGTGCGGCACCCACGGGGCTGCTTCGGGCTCATCTCACATCCGAGGCAAGAACTACAAGTTCGCTTACATTTGGGTCGGAAACTCCGAGACCCAGTGCCCGGGACTCTGCGCCTGGCCCTTCCACCAGCCCCTCTACGGCCCACAGAGTCCACCGCTCGTTGCACCCAACAACGACGTGGGGCTCGACGGCATGGTCATCAACTTGGTGAGCCTCTTGGCCGGAACGGCAACCAACCCTTTCGGAAATGGGTACTTCCAGGGTCCGGCCGAGGCTCCATTAGAGGCTGCTTCGGCTTGTCCCGGGGTTTATGCTAAAGGGGCTTATCCGGGTTACGCCGGAGACCTGTTGGTGGATGCTACGACTGGTGCTAGCTACAATGCCAATGGCGCCAATGGAAGGAAGTACTTGGTTCCTGCTCTGTTTGATCCATCTACCTCAACCTGTAGAAGCCTTGCTGAGACGTCAGACCAAACCCAGCAGCCTATACTATTCCAATATCACAATGGTCCTCTTCTCACTGGAAAAATCTCCATCAATTTGATCTGGTATGGAAAATTCAAGCCCTCCCAGCGCGCAATTGTTTCCGATTTCATCACCTCCCTTTCCACTTCTACTCCATCTACAAACCAACCATCAGTTGCTACATGGTGGAAATCCATTGAGAAATACTACCACCTCTCCAATGCCAAGAAAACCTCATCTCTTTCCCTCTCATTGGGTAACCAAATCCTCGATGAGAGCTACTCTCTGGGCAAGTCCTTATCTGTTGGAAAACACATCAAACAGTTGGCAGCCAAGGGTGGCCAGAGTAATGCCATCAATGTTGTCTTGACCTCATCAGATGTTCTAATTGATGGGTTTTGCTCCAGCAAATGTGGTACACACGGGTCTTCAAAAAGCTCTTTCATCAGAGGCAAGAGCTCCAAGTTTGCTTACATCTGGGTCGGAAACTCCGAGACCCAATGCCCCGGTCAATGTGCTTGGCCATTCCACCAACCTATCTATGGACCACAGACCACACCTTTGGTGGCACCCAACAACGATGTCGGTGTGGACGGTATGATCATCAATCTAGCTAGCCTTTTGGCTGGGACCGTGACCAACCCTTTTGGAAATGGGTTCTACCAGGGTCCCAAAGAGGCTCCATTAGAGGCTGCATCAGCTTGTCCTGGAGTTTATGCTAAAGGAGCTTATCCTGGCTACGCTGGAGACCTCTTGGTTGATGCTACGACTGGTGCCAGCTACAACGCCAATGGTTTGAATGGGAAGAAGTACTTGCTTCCTGCTTTGTTTGACCCTTCAACCTCAACTTGTTCTACTCTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Phi_1

Protein Analysis

617

Amino Acids

65.55

Weight (kDa)

9.05

Isoelectric Point (pI)

27.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 50 - 325 7.6e-124 Phosphate-induced protein 1 conserved region
Phi_1 PF04674 340 - 616 4.3e-123 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35140 AT4G08950
fragaria_vesca FvH4_5g36900 FvH4_5g36910 FvH4_5g36920 FvH4_5g36930
malus_domestica MD08G1185900.v1.1 MD08G1186000.v1.1 MD08G1186100.v1.1 MD08G1186300.v1.1 MD15G1372200.v1.1 MD15G1372400.v1.1
prunus_persica Prupe.1G520600_v2.0.a1 Prupe.1G520700_v2.0.a1 Prupe.1G520800_v2.0.a1
pyrus_communis pycom08g15950 pycom08g15960 pycom08g15970 pycom08g16010 pycom15g33320 pycom15g33330
rosa_chinensis RchiOBHm_Chr0c28g0500911 RchiOBHm_Chr0c28g0500921 RchiOBHm_Chr0c28g0500931 RchiOBHm_Chr0c28g0500981 RchiOBHm_Chr0c28g0501021 RchiOBHm_Chr7g0243461 RchiOBHm_Chr7g0243471 RchiOBHm_Chr7g0243481 RchiOBHm_Chr7g0243521 RchiOBHm_Chr7g0243531 RchiOBHm_Chr7g0243541 RchiOBHm_Chr7g0243581 RchiOBHm_Chr7g0243621 RchiOBHm_Chr7g0243641 RchiOBHm_Chr7g0243671 RchiOBHm_Chr7g0243681 RchiOBHm_Chr7g0243691
rosa_laevigata RLG00000000840 RLG00000000841 RLG00000000842 RLG00000000843 RLG00000000844 RLG00000000845
rosa_multiflora Rmu_sc0000477.1_g000001 Rmu_sc0000477.1_g000003 Rmu_sc0001036.1_g000001 Rmu_sc0001036.1_g000002 Rmu_sc0004062.1_g000001 Rmu_sc0007755.1_g000001 Rmu_sc0008223.1_g000001 Rmu_sc0008473.1_g000006 Rmu_sc0009153.1_g000001 Rmu_sc0009153.1_g000002 Rmu_sc0029916.1_g000001
rosa_roxburghii Rroxscaffold_3G00222020 Rroxscaffold_3G00222030 Rroxscaffold_3G00222040 Rroxscaffold_3G00222060 Rroxscaffold_3G00222100
rosa_rugosa Rorug07G0319800.1 Rorug07G0319900 Rorug07G0320000 Rorug07G0320000 Rorug07G0320100 Rorug07G0320200 Rorug07G0320300
rosa_samantha Rh7BG445800 Rh7BG445900 Rh7BG446000 Rh7BG446100 Rh7BG446200 Rh7BG446300 Rh7BG446400 Rh7BG446500 Rh7CG491600 Rh7CG491700 Rh7CG491800 Rh7CG492000 Rh7CG492100 Rh7CG492200 Rh7CG492300 Rh7CG492500 Rh7CG492600 Rh7DG459900 Rh7DG460000 Rh7DG460100 Rh7DG460200 Rh7DG460300 Rh7DG460400 Rh7DG460600 Rh7DG460700
rosa_wichuraiana Rw7G039570 Rw7G039580 Rw7G039590 Rw7G039620 Rw7G039630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AatII GACGTC 1 cut(s) 989
AccB1I GGYRCC 4 cut(s) 530, 911, 1555, 1760
AccB7I CCANNNNNTGG 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 679
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 1098
AciI CCGC 2 cut(s) 528, 677
AclWI GGATC 1 cut(s) 944
AcoI YGGCCR 4 cut(s) 741, 789, 1327, 1508
AcsI RAATTY 2 cut(s) 381, 1079
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 2 cut(s) 912, 986
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 6 cut(s) 206, 329, 776, 929, 1405, 1799
AfiI CCNNNNNNNGG 8 cut(s) 309, 622, 659, 787, 874, 921, 1240, 1529
AgeI ACCGGT 1 cut(s) 522
AgsI TTSAA 6 cut(s) 131, 217, 1084, 1419, 1786, 1827
AjiI CACGTC 1 cut(s) 700
AjnI CCWGG 6 cut(s) 305, 635, 780, 1650, 1693, 1720
Alw21I GWGCWC 1 cut(s) 1448
Alw26I GTCTC 7 cut(s) 55, 100, 605, 858, 977, 1475, 1728
AlwI GGATC 1 cut(s) 944
Ama87I CYCGRG 2 cut(s) 622, 824
AoxI GGCC 6 cut(s) 638, 661, 741, 789, 1327, 1508
ApeKI GCWGC 5 cut(s) 542, 809, 1003, 1316, 1679
ApoI RAATTY 2 cut(s) 381, 1079
ArsI GACNNNNNNTTYG 4 cut(s) 417, 449, 1531, 1563
AsiGI ACCGGT 1 cut(s) 522
AspLEI GCGC 5 cut(s) 280, 635, 914, 1098, 1100
AspS9I GGNCC 8 cut(s) 164, 639, 662, 785, 1031, 1532, 1617, 1655
AsuC2I CCSGG 6 cut(s) 623, 624, 825, 826, 852, 1494
AsuHPI GGTGA 6 cut(s) 244, 264, 409, 464, 742, 1111
AsuNHI GCTAGC 2 cut(s) 893, 1601
AvaI CYCGRG 2 cut(s) 622, 824
AvaII GGWCC 6 cut(s) 164, 785, 1031, 1532, 1617, 1655
BaeGI GKGCMC 1 cut(s) 623
BalI TGGCCA 2 cut(s) 1329, 1510
BanI GGYRCC 4 cut(s) 530, 911, 1555, 1760
BanII GRGCYC 3 cut(s) 554, 708, 1448
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BbsI GAAGAC 3 cut(s) 320, 345, 1407
Bbv12I GWGCWC 1 cut(s) 1448
BbvI GCAGC 5 cut(s) 529, 796, 1015, 1328, 1666
BccI CCATC 7 cut(s) 197, 961, 1064, 1150, 1165, 1349, 1373
BceAI ACGGC 6 cut(s) 193, 223, 485, 676, 727, 765
BciT130I CCWGG 6 cut(s) 307, 637, 782, 1652, 1695, 1722
BclI TGATCA 1 cut(s) 1587
BcnI CCSGG 6 cut(s) 623, 624, 825, 826, 852, 1494
BcoDI GTCTC 7 cut(s) 55, 100, 605, 858, 977, 1475, 1728
BfaI CTAG 4 cut(s) 81, 894, 1598, 1602
BfmI CTRYAG 1 cut(s) 967
BfoI RGCGCY 1 cut(s) 915
BglI GCCNNNNNGGC 1 cut(s) 749
BisI GCNGC 6 cut(s) 529, 543, 810, 1004, 1317, 1680
BlsI GCNGC 6 cut(s) 530, 544, 811, 1005, 1318, 1681
BmcAI AGTACT 3 cut(s) 329, 929, 1799
Bme18I GGWCC 6 cut(s) 164, 785, 1031, 1532, 1617, 1655
BmeT110I CYCGRG 2 cut(s) 622, 824
BmgBI CACGTC 1 cut(s) 700
BmgT120I GGNCC 8 cut(s) 164, 639, 662, 785, 1031, 1532, 1617, 1655
BmrI ACTGGG 2 cut(s) 16, 610
BmsI GCATC 3 cut(s) 868, 1691, 1738
BmtI GCTAGC 2 cut(s) 897, 1605
BmuI ACTGGG 2 cut(s) 16, 610
BpiI GAAGAC 3 cut(s) 320, 345, 1407
BplI GAGNNNNNCTC 6 cut(s) 383, 415, 1253, 1285, 1255, 1287
BpmI CTGGAG 3 cut(s) 1375, 1716, 1752
BpuMI CCSGG 6 cut(s) 623, 624, 825, 826, 852, 1494
BsaHI GRCGYC 2 cut(s) 912, 986
BsaI GGTCTC 5 cut(s) 55, 605, 858, 1475, 1728
BsaWI WCCGGW 1 cut(s) 522
BsaXI ACNNNNNCTCC 4 cut(s) 1123, 1153, 1187, 1217
Bsc4I CCNNNNNNNGG 8 cut(s) 309, 622, 659, 787, 874, 921, 1240, 1529
Bse118I RCCGGY 2 cut(s) 274, 522
Bse1I ACTGG 6 cut(s) 22, 75, 616, 892, 1048, 1762
BseBI CCWGG 6 cut(s) 307, 637, 782, 1652, 1695, 1722
BseGI GGATG 3 cut(s) 341, 561, 883
BseLI CCNNNNNNNGG 8 cut(s) 309, 622, 659, 787, 874, 921, 1240, 1529
BseMII CTCAG 2 cut(s) 130, 972
BseNI ACTGG 6 cut(s) 22, 75, 616, 892, 1048, 1762
BsePI GCGCGC 1 cut(s) 1096
BseSI GKGCMC 1 cut(s) 623
BseX3I CGGCCG 1 cut(s) 789
BseXI GCAGC 5 cut(s) 529, 796, 1015, 1328, 1666
BseYI CCCAGC 4 cut(s) 141, 999, 1092, 1613
Bsh1236I CGCG 1 cut(s) 1098
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 6 cut(s) 640, 663, 743, 791, 1329, 1510
BshNI GGYRCC 4 cut(s) 530, 911, 1555, 1760
BshTI ACCGGT 1 cut(s) 522
BsiEI CGRYCG 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 1448
BsiHKCI CYCGRG 2 cut(s) 622, 824
BsiSI CCGG 9 cut(s) 275, 523, 623, 744, 788, 825, 851, 861, 1494
BslFI GGGAC 5 cut(s) 397, 639, 807, 1630, 1641
BslI CCNNNNNNNGG 8 cut(s) 309, 622, 659, 787, 874, 921, 1240, 1529
BsmAI GTCTC 7 cut(s) 55, 100, 605, 858, 977, 1475, 1728
BsmBI CGTCTC 1 cut(s) 977
BsmFI GGGAC 5 cut(s) 397, 639, 807, 1630, 1641
BsnI GGCC 6 cut(s) 640, 663, 743, 791, 1329, 1510
Bso31I GGTCTC 5 cut(s) 55, 605, 858, 1475, 1728
BsoBI CYCGRG 2 cut(s) 622, 824
Bsp1286I GDGCHC 4 cut(s) 554, 623, 708, 1448
Bsp143I GATC 4 cut(s) 429, 949, 1065, 1587
BspACI CCGC 2 cut(s) 528, 677
BspANI GGCC 6 cut(s) 640, 663, 743, 791, 1329, 1510
BspCNI CTCAG 2 cut(s) 129, 973
BspFNI CGCG 1 cut(s) 1098
BspOI GCTAGC 2 cut(s) 897, 1605
BspPI GGATC 1 cut(s) 944
BspT107I GGYRCC 4 cut(s) 530, 911, 1555, 1760
BspTNI GGTCTC 5 cut(s) 55, 605, 858, 1475, 1728
BsrBI CCGCTC 1 cut(s) 679
BsrFI RCCGGY 2 cut(s) 274, 522
BsrI ACTGG 6 cut(s) 22, 75, 616, 892, 1048, 1762
BssAI RCCGGY 2 cut(s) 274, 522
BssHII GCGCGC 1 cut(s) 1096
BssMI GATC 4 cut(s) 429, 949, 1065, 1587
BssNI GRCGYC 2 cut(s) 912, 986
BssT1I CCWWGG 2 cut(s) 99, 1320
Bst2UI CCWGG 6 cut(s) 307, 637, 782, 1652, 1695, 1722
Bst4CI ACNGT 6 cut(s) 164, 298, 509, 1311, 1583, 1621
Bst6I CTCTTC 3 cut(s) 62, 174, 1041
BstACI GRCGYC 2 cut(s) 912, 986
BstC8I GCNNGC 5 cut(s) 222, 895, 1098, 1603, 1765
BstDEI CTNAG 3 cut(s) 43, 116, 981
BstDSI CCRYGG 1 cut(s) 535
BstEII GGTNACC 2 cut(s) 452, 1244
BstF5I GGATG 3 cut(s) 341, 561, 883
BstFNI CGCG 1 cut(s) 1098
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 5 cut(s) 280, 635, 914, 1098, 1100
BstKTI GATC 4 cut(s) 432, 952, 1068, 1590
BstMAI GTCTC 7 cut(s) 55, 100, 605, 858, 977, 1475, 1728
BstMBI GATC 4 cut(s) 429, 949, 1065, 1587
BstMCI CGRYCG 1 cut(s) 792
BstNI CCWGG 6 cut(s) 307, 637, 782, 1652, 1695, 1722
BstPI GGTNACC 2 cut(s) 452, 1244
BstSFI CTRYAG 1 cut(s) 967
BstSLI GKGCMC 1 cut(s) 623
BstUI CGCG 1 cut(s) 1098
BstV1I GCAGC 5 cut(s) 529, 796, 1015, 1328, 1666
BstV2I GAAGAC 3 cut(s) 320, 345, 1407
BstXI CCANNNNNNTGG 1 cut(s) 1549
BstZI CGGCCG 1 cut(s) 789
BsuRI GGCC 6 cut(s) 640, 663, 743, 791, 1329, 1510
BtgI CCRYGG 1 cut(s) 535
BtrI CACGTC 1 cut(s) 700
BtsCI GGATG 3 cut(s) 341, 561, 883
BtsIMutI CAGTG 4 cut(s) 68, 124, 623, 1041
Cac8I GCNNGC 5 cut(s) 222, 895, 1098, 1603, 1765
CfoI GCGC 5 cut(s) 280, 635, 914, 1098, 1100
Cfr10I RCCGGY 2 cut(s) 274, 522
Cfr13I GGNCC 8 cut(s) 164, 639, 662, 785, 1031, 1532, 1617, 1655
Cfr9I CCCGGG 2 cut(s) 622, 824
CsiI ACCWGGT 1 cut(s) 305
Csp6I GTAC 6 cut(s) 205, 328, 775, 928, 1404, 1798
CspAI ACCGGT 1 cut(s) 522
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 3 cut(s) 517, 715, 1170
CviQI GTAC 6 cut(s) 205, 328, 775, 928, 1404, 1798
DdeI CTNAG 3 cut(s) 43, 116, 981
DinI GGCGCC 1 cut(s) 913
DpnI GATC 4 cut(s) 431, 951, 1067, 1589
DpnII GATC 4 cut(s) 429, 949, 1065, 1587
DraIII CACNNNGTG 1 cut(s) 309
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 4 cut(s) 741, 789, 1327, 1508
EagI CGGCCG 1 cut(s) 789
Eam1104I CTCTTC 3 cut(s) 62, 174, 1041
EarI CTCTTC 3 cut(s) 62, 174, 1041
Ecl136II GAGCTC 1 cut(s) 1446
EclXI CGGCCG 1 cut(s) 789
Eco130I CCWWGG 2 cut(s) 99, 1320
Eco24I GRGCYC 3 cut(s) 554, 708, 1448
Eco31I GGTCTC 5 cut(s) 55, 605, 858, 1475, 1728
Eco47I GGWCC 6 cut(s) 164, 785, 1031, 1532, 1617, 1655
Eco52I CGGCCG 1 cut(s) 789
Eco53kI GAGCTC 1 cut(s) 1446
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 2 cut(s) 622, 824
Eco91I GGTNACC 2 cut(s) 452, 1244
EcoICRI GAGCTC 1 cut(s) 1446
EcoO109I RGGNCCY 1 cut(s) 1655
EcoO65I GGTNACC 2 cut(s) 452, 1244
EcoRII CCWGG 6 cut(s) 305, 635, 780, 1650, 1693, 1720
EcoT14I CCWWGG 2 cut(s) 99, 1320
EcoT38I GRGCYC 3 cut(s) 554, 708, 1448
EgeI GGCGCC 1 cut(s) 913
EheI GGCGCC 1 cut(s) 913
ErhI CCWWGG 2 cut(s) 99, 1320
Esp3I CGTCTC 1 cut(s) 977
FaeI CATG 3 cut(s) 520, 718, 1173
FalI AAGNNNNNCTT 2 cut(s) 1784, 1816
FaqI GGGAC 5 cut(s) 397, 639, 807, 1630, 1641
FatI CATG 3 cut(s) 516, 714, 1169
FbaI TGATCA 1 cut(s) 1587
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 6 cut(s) 529, 543, 810, 1004, 1317, 1680
FokI GGATG 3 cut(s) 328, 548, 890
FriOI GRGCYC 3 cut(s) 554, 708, 1448
Fsp4HI GCNGC 6 cut(s) 529, 543, 810, 1004, 1317, 1680
FspBI CTAG 4 cut(s) 81, 894, 1598, 1602
GlaI GCGC 5 cut(s) 279, 634, 913, 1097, 1099
GluI GCNGC 6 cut(s) 529, 543, 810, 1004, 1317, 1680
GsaI CCCAGC 4 cut(s) 145, 1003, 1096, 1617
GsuI CTGGAG 3 cut(s) 1375, 1716, 1752
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 6 cut(s) 640, 663, 743, 791, 1329, 1510
HapII CCGG 9 cut(s) 275, 523, 623, 744, 788, 825, 851, 861, 1494
HhaI GCGC 5 cut(s) 280, 635, 914, 1098, 1100
Hin1I GRCGYC 2 cut(s) 912, 986
Hin1II CATG 3 cut(s) 520, 718, 1173
Hin6I GCGC 5 cut(s) 278, 633, 912, 1096, 1098
HinP1I GCGC 5 cut(s) 278, 633, 912, 1096, 1098
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 2 cut(s) 627, 670
HpaII CCGG 9 cut(s) 275, 523, 623, 744, 788, 825, 851, 861, 1494
HphI GGTGA 6 cut(s) 244, 264, 409, 464, 742, 1111
Hpy166II GTNNAC 4 cut(s) 505, 674, 1406, 1579
Hpy188III TCNNGA 4 cut(s) 131, 373, 481, 1354
Hpy8I GTNNAC 4 cut(s) 505, 674, 1406, 1579
Hpy99I CGWCG 6 cut(s) 302, 392, 506, 509, 701, 713
HpyAV CCTTC 3 cut(s) 652, 915, 1833
HpyCH4III ACNGT 6 cut(s) 164, 298, 509, 1311, 1583, 1621
HpyCH4IV ACGT 3 cut(s) 474, 699, 986
HpyCH4V TGCA 3 cut(s) 516, 686, 1682
HpyF3I CTNAG 3 cut(s) 43, 116, 981
HpySE526I ACGT 3 cut(s) 474, 699, 986
Hsp92I GRCGYC 2 cut(s) 912, 986
Hsp92II CATG 3 cut(s) 520, 718, 1173
HspAI GCGC 5 cut(s) 278, 633, 912, 1096, 1098
KasI GGCGCC 1 cut(s) 911
KflI GGGWCCC 1 cut(s) 1655
Ksp22I TGATCA 1 cut(s) 1587
Kzo9I GATC 4 cut(s) 429, 949, 1065, 1587
LmnI GCTCC 6 cut(s) 150, 802, 1394, 1451, 1672, 1712
Lsp1109I GCAGC 5 cut(s) 529, 796, 1015, 1328, 1666
LweI GCATC 3 cut(s) 868, 1691, 1738
MabI ACCWGGT 1 cut(s) 305
MaeI CTAG 4 cut(s) 81, 894, 1598, 1602
MaeII ACGT 3 cut(s) 474, 699, 986
MaeIII GTNAC 4 cut(s) 452, 854, 1244, 1621
MalI GATC 4 cut(s) 431, 951, 1067, 1589
MbiI CCGCTC 1 cut(s) 679
MboI GATC 4 cut(s) 429, 949, 1065, 1587
MfeI CAATTG 1 cut(s) 1101
MhlI GDGCHC 4 cut(s) 554, 623, 708, 1448
MlsI TGGCCA 2 cut(s) 1329, 1510
MluCI AATT 5 cut(s) 381, 1060, 1079, 1101, 1374
MluNI TGGCCA 2 cut(s) 1329, 1510
Mly113I GGCGCC 1 cut(s) 912
MlyI GAGTC 2 cut(s) 621, 679
MmeI TCCRAC 5 cut(s) 309, 411, 580, 1273, 1450
Mox20I TGGCCA 2 cut(s) 1329, 1510
MscI TGGCCA 2 cut(s) 1329, 1510
MseI TTAA 1 cut(s) 150
Msp20I TGGCCA 2 cut(s) 1329, 1510
MspI CCGG 9 cut(s) 275, 523, 623, 744, 788, 825, 851, 861, 1494
MunI CAATTG 1 cut(s) 1101
MvaI CCWGG 6 cut(s) 307, 637, 782, 1652, 1695, 1722
MvnI CGCG 1 cut(s) 1098
NarI GGCGCC 1 cut(s) 912
NciI CCSGG 6 cut(s) 623, 624, 825, 826, 852, 1494
NdeII GATC 4 cut(s) 429, 949, 1065, 1587
NheI GCTAGC 2 cut(s) 893, 1601
NlaIII CATG 3 cut(s) 520, 718, 1173
NmeAIII GCCGAG 1 cut(s) 817
NmuCI GTSAC 2 cut(s) 452, 1621
PauI GCGCGC 1 cut(s) 1096
PcsI WCGNNNNNNNCGW 4 cut(s) 471, 606, 705, 1476
PflMI CCANNNNNTGG 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 1693
PinAI ACCGGT 1 cut(s) 522
PkrI GCNGC 6 cut(s) 530, 544, 811, 1005, 1318, 1681
PleI GAGTC 2 cut(s) 621, 678
PluTI GGCGCC 1 cut(s) 915
PpsI GAGTC 2 cut(s) 621, 678
PpuMI RGGWCCY 1 cut(s) 1655
Psp124BI GAGCTC 1 cut(s) 1448
Psp5II RGGWCCY 1 cut(s) 1655
Psp6I CCWGG 6 cut(s) 305, 635, 780, 1650, 1693, 1720
PspEI GGTNACC 2 cut(s) 452, 1244
PspFI CCCAGC 4 cut(s) 141, 999, 1092, 1613
PspGI CCWGG 6 cut(s) 305, 635, 780, 1650, 1693, 1720
PspPI GGNCC 8 cut(s) 164, 639, 662, 785, 1031, 1532, 1617, 1655
PspPPI RGGWCCY 1 cut(s) 1655
PteI GCGCGC 1 cut(s) 1096
RsaI GTAC 6 cut(s) 206, 329, 776, 929, 1405, 1799
RsaNI GTAC 6 cut(s) 205, 328, 775, 928, 1404, 1798
SacI GAGCTC 1 cut(s) 1448
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 6 cut(s) 529, 543, 810, 1004, 1317, 1680
Sau3AI GATC 4 cut(s) 429, 949, 1065, 1587
Sau96I GGNCC 8 cut(s) 164, 639, 662, 785, 1031, 1532, 1617, 1655
ScaI AGTACT 3 cut(s) 329, 929, 1799
SchI GAGTC 2 cut(s) 621, 679
SduI GDGCHC 4 cut(s) 554, 623, 708, 1448
SexAI ACCWGGT 1 cut(s) 305
SfaNI GCATC 3 cut(s) 868, 1691, 1738
SfcI CTRYAG 1 cut(s) 967
SfoI GGCGCC 1 cut(s) 913
SgrAI CRCCGGYG 1 cut(s) 274
SgrDI CGTCGACG 1 cut(s) 503
SinI GGWCC 6 cut(s) 164, 785, 1031, 1532, 1617, 1655
SmaI CCCGGG 2 cut(s) 624, 826
Sse9I AATT 5 cut(s) 381, 1060, 1079, 1101, 1374
SsiI CCGC 2 cut(s) 528, 677
SspDI GGCGCC 1 cut(s) 911
SspMI CTAG 4 cut(s) 81, 894, 1598, 1602
SstI GAGCTC 1 cut(s) 1448
StyI CCWWGG 2 cut(s) 99, 1320
TaaI ACNGT 6 cut(s) 164, 298, 509, 1311, 1583, 1621
TaiI ACGT 3 cut(s) 477, 702, 989
TaqI TCGA 3 cut(s) 504, 708, 1257
TasI AATT 5 cut(s) 381, 1060, 1079, 1101, 1374
TatI WGTACW 3 cut(s) 327, 927, 1797
TauI GCSGC 1 cut(s) 531
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 4 cut(s) 75, 124, 623, 1048
TseFI GTSAC 2 cut(s) 452, 1621
TseI GCWGC 5 cut(s) 542, 809, 1003, 1316, 1679
Tsp45I GTSAC 2 cut(s) 452, 1621
TspDTI ATGAA 4 cut(s) 238, 533, 1105, 1420
TspMI CCCGGG 2 cut(s) 622, 824
TspRI CASTG 4 cut(s) 75, 124, 623, 1048
Van91I CCANNNNNTGG 1 cut(s) 309
VpaK11BI GGWCC 6 cut(s) 164, 785, 1031, 1532, 1617, 1655
XapI RAATTY 2 cut(s) 381, 1079
XcmI CCANNNNNNNNNTGG 4 cut(s) 697, 1025, 1526, 1549
XmaI CCCGGG 2 cut(s) 622, 824
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 4 cut(s) 81, 894, 1598, 1602
ZraI GACGTC 1 cut(s) 987
ZrmI AGTACT 3 cut(s) 329, 929, 1799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.