MD15G1372200.v1.1

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000422
Physical Location & Seq
Forward (+)
45378562 .. 45379554
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1372200.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGGTTTTAATTCTGGTCAATGGCTTTTGAAGCTGTTTGTTGTGGCCACCTTGTTCCAAGTTTCATTCGGTTCCAGCAGATTGTTCCAAGTCTCTTTGGCAACAAGGAAGCTGAGTGAGCTGGTCCAAGACCAAACCCAGCTCCTCAAATACCACAACGGACCTCTCCTGTCCGGCAAAATTACCATCAACCTCATCTGGTACGGTAACTTCAAGCCCTCCCAGAAAGCCATCGTTTCCGATTTCCTTTCCTCCTTGTCCTCGCCCTCCAAAACCACCCAACCCTCCGTCGCCGCCTGGTGGAAGACCACCGAGAAATACTACCACCTCACCTCCAACAAAAAGTCCAACTCTCTCTCCCTCTCTCTGGGAAGACAGATTCTCGACCAAAAATACTCCCTCGGCAAATCCCTCACCACCAAACAGATTGTGGCATTGGCCGCAAAGGGCGACCAGAAAAACGCCATCAATGTTGTCCTGACATCCGCCGACGTCGCCGTCGATGGGTTCTGTATGAACCGGTGCGGGACCCACGGGTCTGCCTCCGGCGCTACCAAAACCGGACACGTCAAAGGCAGCAAGAACTATAAGTTCGCATACATCTGGGTCGGAAACTCGGAGACCCAATGCCCGGGGCAGTGCGCGTGGCCATTCCACCAGCCTATCTATGGACCGCAGAGCCCACCCCTTGTGGCTCCCAACAACGATGTGGGCCTCGACGGCATGGTCATCAACCTTGCTAGCCTTCTTGCCGGGACAGCCACCAACCCATTCGGGAATGGATACTTTCAGGGCCCAGCCGAGGCGCCTTTGGAGGCGGCTTCGGCCTGCCCTGGAGTTTACGGGAAAGGGGCTTATCCTGGTTATGCCGGGGACTTGCTGCAGGACGCCACCACAGGTGCCAGCTACAATGCCAATGGTGCTAATGGAAGGAAGTACTTGCTCCCTGCACTATATAATCCTGCAACTTCATCTTGTTCCACTTTGGTTTGA

Protein Analysis

331

Amino Acids

35.15

Weight (kDa)

9.36

Isoelectric Point (pI)

25.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 49 - 329 4.3e-127 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35140 AT4G08950
fragaria_vesca FvH4_5g36900 FvH4_5g36910 FvH4_5g36920 FvH4_5g36930
malus_domestica MD08G1185900.v1.1 MD08G1186000.v1.1 MD08G1186100.v1.1 MD08G1186300.v1.1 MD15G1372200.v1.1 MD15G1372400.v1.1
prunus_persica Prupe.1G520600_v2.0.a1 Prupe.1G520700_v2.0.a1 Prupe.1G520800_v2.0.a1
pyrus_communis pycom08g15950 pycom08g15960 pycom08g15970 pycom08g16010 pycom15g33320 pycom15g33330
rosa_chinensis RchiOBHm_Chr0c28g0500911 RchiOBHm_Chr0c28g0500921 RchiOBHm_Chr0c28g0500931 RchiOBHm_Chr0c28g0500981 RchiOBHm_Chr0c28g0501021 RchiOBHm_Chr7g0243461 RchiOBHm_Chr7g0243471 RchiOBHm_Chr7g0243481 RchiOBHm_Chr7g0243521 RchiOBHm_Chr7g0243531 RchiOBHm_Chr7g0243541 RchiOBHm_Chr7g0243581 RchiOBHm_Chr7g0243621 RchiOBHm_Chr7g0243641 RchiOBHm_Chr7g0243671 RchiOBHm_Chr7g0243681 RchiOBHm_Chr7g0243691
rosa_laevigata RLG00000000840 RLG00000000841 RLG00000000842 RLG00000000843 RLG00000000844 RLG00000000845
rosa_multiflora Rmu_sc0000477.1_g000001 Rmu_sc0000477.1_g000003 Rmu_sc0001036.1_g000001 Rmu_sc0001036.1_g000002 Rmu_sc0004062.1_g000001 Rmu_sc0007755.1_g000001 Rmu_sc0008223.1_g000001 Rmu_sc0008473.1_g000006 Rmu_sc0009153.1_g000001 Rmu_sc0009153.1_g000002 Rmu_sc0029916.1_g000001
rosa_roxburghii Rroxscaffold_3G00222020 Rroxscaffold_3G00222030 Rroxscaffold_3G00222040 Rroxscaffold_3G00222060 Rroxscaffold_3G00222100
rosa_rugosa Rorug07G0319800.1 Rorug07G0319900 Rorug07G0320000 Rorug07G0320000 Rorug07G0320100 Rorug07G0320200 Rorug07G0320300
rosa_samantha Rh7BG445800 Rh7BG445900 Rh7BG446000 Rh7BG446100 Rh7BG446200 Rh7BG446300 Rh7BG446400 Rh7BG446500 Rh7CG491600 Rh7CG491700 Rh7CG491800 Rh7CG492000 Rh7CG492100 Rh7CG492200 Rh7CG492300 Rh7CG492500 Rh7CG492600 Rh7DG459900 Rh7DG460000 Rh7DG460100 Rh7DG460200 Rh7DG460300 Rh7DG460400 Rh7DG460600 Rh7DG460700
rosa_wichuraiana Rw7G039570 Rw7G039580 Rw7G039590 Rw7G039620 Rw7G039630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 497, 535, 725
AatII GACGTC 1 cut(s) 495
AccB1I GGYRCC 2 cut(s) 805, 899
AccII CGCG 1 cut(s) 644
AciI CCGC 6 cut(s) 294, 441, 486, 525, 674, 818
AcoI YGGCCR 3 cut(s) 45, 438, 647
AcyI GRCGYC 3 cut(s) 492, 806, 888
AfaI GTAC 2 cut(s) 203, 938
AfiI CCNNNNNNNGG 6 cut(s) 300, 367, 504, 631, 668, 802
AflIII ACRYGT 1 cut(s) 565
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 2 cut(s) 31, 214
AjiI CACGTC 1 cut(s) 568
AjnI CCWGG 3 cut(s) 296, 832, 859
AluBI AGCT 5 cut(s) 34, 112, 121, 142, 906
AluI AGCT 5 cut(s) 34, 112, 121, 142, 906
Alw26I GTCTC 2 cut(s) 97, 614
Ama87I CYCGRG 1 cut(s) 631
AoxI GGCC 6 cut(s) 45, 438, 647, 712, 793, 825
ApaI GGGCCC 1 cut(s) 797
ApeKI GCWGC 2 cut(s) 576, 880
AsiGI ACCGGT 1 cut(s) 519
AspLEI GCGC 3 cut(s) 551, 644, 808
AspS9I GGNCC 7 cut(s) 124, 161, 528, 671, 712, 793, 794
AsuC2I CCSGG 4 cut(s) 632, 633, 754, 871
AsuHPI GGTGA 2 cut(s) 322, 406
AsuNHI GCTAGC 1 cut(s) 740
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 4 cut(s) 124, 161, 528, 671
BaeGI GKGCMC 1 cut(s) 797
BalI TGGCCA 2 cut(s) 47, 649
BanI GGYRCC 2 cut(s) 805, 899
BanII GRGCYC 2 cut(s) 683, 797
BarI GAAGNNNNNNTAC 2 cut(s) 194, 226
BbsI GAAGAC 2 cut(s) 311, 379
BbvI GCAGC 2 cut(s) 588, 867
BccI CCATC 4 cut(s) 194, 239, 473, 497
BceAI ACGGC 2 cut(s) 482, 736
BciT130I CCWGG 3 cut(s) 298, 834, 861
BciVI GTATCC 1 cut(s) 776
BcnI CCSGG 4 cut(s) 632, 633, 754, 871
BcoDI GTCTC 2 cut(s) 97, 614
BfaI CTAG 1 cut(s) 741
BfmI CTRYAG 1 cut(s) 881
BfoI RGCGCY 2 cut(s) 552, 809
BfuI GTATCC 1 cut(s) 776
BglI GCCNNNNNGGC 1 cut(s) 720
BisI GCNGC 5 cut(s) 294, 441, 577, 819, 881
BlsI GCNGC 5 cut(s) 295, 442, 578, 820, 882
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 7 cut(s) 298, 632, 633, 754, 834, 861, 871
Bme18I GGWCC 4 cut(s) 124, 161, 528, 671
BmeT110I CYCGRG 1 cut(s) 631
BmgBI CACGTC 1 cut(s) 568
BmgT120I GGNCC 7 cut(s) 124, 161, 528, 671, 712, 793, 794
BmiI GGNNCC 7 cut(s) 73, 529, 530, 696, 795, 807, 901
BmrFI CCNGG 7 cut(s) 298, 632, 633, 754, 834, 861, 871
BmtI GCTAGC 1 cut(s) 744
BpiI GAAGAC 2 cut(s) 311, 379
BpmI CTGGAG 1 cut(s) 855
BpuMI CCSGG 4 cut(s) 632, 633, 754, 871
BsaHI GRCGYC 3 cut(s) 492, 806, 888
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 7 cut(s) 400, 532, 631, 632, 801, 832, 870
BsaWI WCCGGW 2 cut(s) 519, 560
BsaXI ACNNNNNCTCC 4 cut(s) 317, 341, 347, 371
Bsc4I CCNNNNNNNGG 6 cut(s) 300, 367, 504, 631, 668, 802
Bse118I RCCGGY 1 cut(s) 519
BseBI CCWGG 3 cut(s) 298, 834, 861
BseDI CCNNGG 7 cut(s) 400, 532, 631, 632, 801, 832, 870
BseGI GGATG 1 cut(s) 482
BseLI CCNNNNNNNGG 6 cut(s) 300, 367, 504, 631, 668, 802
BseMII CTCAG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 134
BseSI GKGCMC 1 cut(s) 797
BseXI GCAGC 2 cut(s) 588, 867
BseYI CCCAGC 2 cut(s) 138, 796
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 1 cut(s) 644
BshFI GGCC 6 cut(s) 47, 440, 649, 714, 795, 827
BshNI GGYRCC 2 cut(s) 805, 899
BshTI ACCGGT 1 cut(s) 519
BsiHKCI CYCGRG 1 cut(s) 631
BsiSI CCGG 7 cut(s) 174, 520, 546, 561, 632, 753, 870
BslFI GGGAC 3 cut(s) 541, 769, 887
BslI CCNNNNNNNGG 6 cut(s) 300, 367, 504, 631, 668, 802
BsmAI GTCTC 2 cut(s) 97, 614
BsmFI GGGAC 3 cut(s) 541, 769, 887
BsnI GGCC 6 cut(s) 47, 440, 649, 714, 795, 827
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 631
Bsp120I GGGCCC 1 cut(s) 793
Bsp1286I GDGCHC 2 cut(s) 683, 797
BspACI CCGC 6 cut(s) 294, 441, 486, 525, 674, 818
BspANI GGCC 6 cut(s) 47, 440, 649, 714, 795, 827
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 644
BspLI GGNNCC 7 cut(s) 73, 529, 530, 696, 795, 807, 901
BspMAI CTGCAG 1 cut(s) 885
BspOI GCTAGC 1 cut(s) 744
BspT107I GGYRCC 2 cut(s) 805, 899
BspTNI GGTCTC 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 7 cut(s) 400, 532, 631, 632, 801, 832, 870
BssNI GRCGYC 3 cut(s) 492, 806, 888
Bst2UI CCWGG 3 cut(s) 298, 834, 861
Bst4CI ACNGT 1 cut(s) 206
BstACI GRCGYC 3 cut(s) 492, 806, 888
BstC8I GCNNGC 3 cut(s) 742, 829, 904
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 1 cut(s) 482
BstFNI CGCG 1 cut(s) 644
BstH2I RGCGCY 2 cut(s) 552, 809
BstHHI GCGC 3 cut(s) 551, 644, 808
BstMAI GTCTC 2 cut(s) 97, 614
BstMWI GCNNNNNNNGC 9 cut(s) 31, 118, 440, 494, 548, 720, 758, 824, 920
BstNI CCWGG 3 cut(s) 298, 834, 861
BstSCI CCNGG 7 cut(s) 296, 630, 631, 752, 832, 859, 869
BstSFI CTRYAG 1 cut(s) 881
BstSLI GKGCMC 1 cut(s) 797
BstUI CGCG 1 cut(s) 644
BstV1I GCAGC 2 cut(s) 588, 867
BstV2I GAAGAC 2 cut(s) 311, 379
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 6 cut(s) 47, 440, 649, 714, 795, 827
BtgI CCRYGG 1 cut(s) 532
BtrI CACGTC 1 cut(s) 568
BtsCI GGATG 1 cut(s) 482
BtsI GCAGTG 1 cut(s) 644
BtsIMutI CAGTG 1 cut(s) 644
Cac8I GCNNGC 3 cut(s) 742, 829, 904
CfoI GCGC 3 cut(s) 551, 644, 808
Cfr10I RCCGGY 1 cut(s) 519
Cfr13I GGNCC 7 cut(s) 124, 161, 528, 671, 712, 793, 794
Cfr9I CCCGGG 1 cut(s) 631
CseI GACGC 1 cut(s) 896
Csp6I GTAC 2 cut(s) 202, 937
CspAI ACCGGT 1 cut(s) 519
CviAII CATG 1 cut(s) 724
CviQI GTAC 2 cut(s) 202, 937
DdeI CTNAG 1 cut(s) 113
DinI GGCGCC 1 cut(s) 807
DrdI GACNNNNNNGTC 3 cut(s) 497, 535, 725
DseDI GACNNNNNNGTC 3 cut(s) 497, 535, 725
EaeI YGGCCR 3 cut(s) 45, 438, 647
EciI GGCGGA 1 cut(s) 475
Eco24I GRGCYC 2 cut(s) 683, 797
Eco31I GGTCTC 1 cut(s) 614
Eco47I GGWCC 4 cut(s) 124, 161, 528, 671
Eco88I CYCGRG 1 cut(s) 631
EcoO109I RGGNCCY 2 cut(s) 528, 793
EcoRII CCWGG 3 cut(s) 296, 832, 859
EcoT38I GRGCYC 2 cut(s) 683, 797
EgeI GGCGCC 1 cut(s) 807
EheI GGCGCC 1 cut(s) 807
FaeI CATG 1 cut(s) 727
FaiI YATR 8 cut(s) 515, 588, 598, 669, 725, 867, 955, 957
FaqI GGGAC 3 cut(s) 541, 769, 887
FatI CATG 1 cut(s) 723
FauI CCCGC 1 cut(s) 518
Fnu4HI GCNGC 5 cut(s) 294, 441, 577, 819, 881
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 2 cut(s) 683, 797
Fsp4HI GCNGC 5 cut(s) 294, 441, 577, 819, 881
FspBI CTAG 1 cut(s) 741
GlaI GCGC 3 cut(s) 550, 643, 807
GluI GCNGC 5 cut(s) 294, 441, 577, 819, 881
GsaI CCCAGC 2 cut(s) 142, 800
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 2 cut(s) 552, 809
HaeIII GGCC 6 cut(s) 47, 440, 649, 714, 795, 827
HapII CCGG 7 cut(s) 174, 520, 546, 561, 632, 753, 870
HgaI GACGC 1 cut(s) 896
HhaI GCGC 3 cut(s) 551, 644, 808
Hin1I GRCGYC 3 cut(s) 492, 806, 888
Hin1II CATG 1 cut(s) 727
Hin6I GCGC 3 cut(s) 549, 642, 806
HinP1I GCGC 3 cut(s) 549, 642, 806
HinfI GANTC 1 cut(s) 379
HpaII CCGG 7 cut(s) 174, 520, 546, 561, 632, 753, 870
HphI GGTGA 2 cut(s) 322, 406
Hpy166II GTNNAC 1 cut(s) 841
Hpy188I TCNGA 3 cut(s) 241, 611, 619
Hpy188III TCNNGA 3 cut(s) 383, 478, 775
Hpy8I GTNNAC 1 cut(s) 841
Hpy99I CGWCG 5 cut(s) 293, 494, 497, 503, 722
HpyAV CCTTC 2 cut(s) 755, 924
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4IV ACGT 2 cut(s) 492, 567
HpyCH4V TGCA 3 cut(s) 883, 950, 965
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 118, 440, 494, 548, 720, 758, 824, 920
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 2 cut(s) 492, 567
Hsp92I GRCGYC 3 cut(s) 492, 806, 888
Hsp92II CATG 1 cut(s) 727
HspAI GCGC 3 cut(s) 549, 642, 806
KasI GGCGCC 1 cut(s) 805
KflI GGGWCCC 1 cut(s) 528
LmnI GCTCC 3 cut(s) 147, 700, 948
Lsp1109I GCAGC 2 cut(s) 588, 867
MaeI CTAG 1 cut(s) 741
MaeII ACGT 2 cut(s) 492, 567
MaeIII GTNAC 1 cut(s) 206
MboII GAAGA 2 cut(s) 316, 384
MhlI GDGCHC 2 cut(s) 683, 797
MlsI TGGCCA 2 cut(s) 47, 649
MluCI AATT 2 cut(s) 10, 180
MluNI TGGCCA 2 cut(s) 47, 649
Mly113I GGCGCC 1 cut(s) 806
MmeI TCCRAC 3 cut(s) 360, 372, 589
Mox20I TGGCCA 2 cut(s) 47, 649
MscI TGGCCA 2 cut(s) 47, 649
MseI TTAA 1 cut(s) 9
Msp20I TGGCCA 2 cut(s) 47, 649
MspI CCGG 7 cut(s) 174, 520, 546, 561, 632, 753, 870
MspR9I CCNGG 7 cut(s) 298, 632, 633, 754, 834, 861, 871
MvaI CCWGG 3 cut(s) 298, 834, 861
MvnI CGCG 1 cut(s) 644
MwoI GCNNNNNNNGC 9 cut(s) 31, 118, 440, 494, 548, 720, 758, 824, 920
NarI GGCGCC 1 cut(s) 806
NciI CCSGG 4 cut(s) 632, 633, 754, 871
NheI GCTAGC 1 cut(s) 740
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 7 cut(s) 73, 529, 530, 696, 795, 807, 901
NmeAIII GCCGAG 2 cut(s) 381, 826
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PfeI GAWTC 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 5 cut(s) 295, 442, 578, 820, 882
PluTI GGCGCC 1 cut(s) 809
PpuMI RGGWCCY 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 528
Psp6I CCWGG 3 cut(s) 296, 832, 859
PspFI CCCAGC 2 cut(s) 138, 796
PspGI CCWGG 3 cut(s) 296, 832, 859
PspN4I GGNNCC 7 cut(s) 73, 529, 530, 696, 795, 807, 901
PspOMI GGGCCC 1 cut(s) 793
PspPI GGNCC 7 cut(s) 124, 161, 528, 671, 712, 793, 794
PspPPI RGGWCCY 1 cut(s) 528
PstI CTGCAG 1 cut(s) 885
RsaI GTAC 2 cut(s) 203, 938
RsaNI GTAC 2 cut(s) 202, 937
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 5 cut(s) 294, 441, 577, 819, 881
Sau96I GGNCC 7 cut(s) 124, 161, 528, 671, 712, 793, 794
ScaI AGTACT 1 cut(s) 938
ScrFI CCNGG 7 cut(s) 298, 632, 633, 754, 834, 861, 871
SduI GDGCHC 2 cut(s) 683, 797
SfcI CTRYAG 1 cut(s) 881
SfoI GGCGCC 1 cut(s) 807
SinI GGWCC 4 cut(s) 124, 161, 528, 671
SmaI CCCGGG 1 cut(s) 633
Sse9I AATT 2 cut(s) 10, 180
SsiI CCGC 6 cut(s) 294, 441, 486, 525, 674, 818
SspDI GGCGCC 1 cut(s) 805
SspMI CTAG 1 cut(s) 741
StyD4I CCNGG 7 cut(s) 296, 630, 631, 752, 832, 859, 869
TaaI ACNGT 1 cut(s) 206
TaiI ACGT 2 cut(s) 495, 570
TaqI TCGA 3 cut(s) 384, 501, 717
TasI AATT 2 cut(s) 10, 180
TatI WGTACW 1 cut(s) 936
TauI GCSGC 3 cut(s) 296, 443, 821
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 644
TseI GCWGC 2 cut(s) 576, 880
TspDTI ATGAA 3 cut(s) 54, 530, 960
TspGWI ACGGA 2 cut(s) 174, 277
TspMI CCCGGG 1 cut(s) 631
TspRI CASTG 1 cut(s) 644
VpaK11BI GGWCC 4 cut(s) 124, 161, 528, 671
XcmI CCANNNNNNNNNTGG 3 cut(s) 427, 665, 706
XmaI CCCGGG 1 cut(s) 631
XspI CTAG 1 cut(s) 741
ZraI GACGTC 1 cut(s) 493
ZrmI AGTACT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.