pycom15g33330

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000422
Physical Location & Seq
Forward (+)
32709095 .. 32710054
960 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33330.1

Sequence Viewer

Length: 960 bp
ATGGCAGCTTTTGCGATTTTGCAACTAGTCCTTGTTATTTCTTTGTTTCATTTCAGCTCAGCAGCCAGGAACCTTGCTGAAACTTCCGATAAAAACCAAATGCAATTCCAGTACCACAACGGCCCTCTCCTCACCGGACAATTCTCCGTCAACTTGATCTGGTACGGCCGCTTCACCCCCTCCCAGCGTGCAATCGTTTCCGATTTCTTCACCTCCCTATCCAAACCCACCTCCGACCAACCATCCGTCGCCACGTGGTGGAAGTCCATAGAGAAATACTACCACCTCGCGCATTTGAACACACCCTCTTCATCTCTCTCCCTCTCTCTGGGAACCCAAATTCTTGATGAAAATTACTCTCTTGGAAAATCCCTCACTTCCCGCCAAATCCAACAGCTGGCCTCAAAGGGTGCACTAAACTCCGCAATCAATGTCGTTTTGACATCGTCCGATGTTCTTGTCGACGGGTTCTGCATGAACAGTTGCGGCACCCACGGCTCATCCAACGACGCTAGTCAAATCAGAGGATCAAAATCCAACAAGTTCGCTTACATTTGGGTCGGAAACTCAGAGAGCCAGTGCCCGGGGCAGTGCGCGTGGCCATTCCACCAGCCCATCTATGGACCCCAGAGCCCACCCCTTGTGGCCCCCAACAACGACGTGGGCCTGGACGGCATGGTGATCAACCTTCGAGTGTGGCTAGCCTTTTGGCGGGGACCGCGACCAACCCTTTCGGAAACGGCTACTTCCAGGGTCCGAAGGAGGCTCCTTTGGAAGCTGCTTCTGCTTGCCCTGGGGTCTACGGCAAAGGGGCCTATCCTGGTTATGCTGGGGACCTACTGGTGGACCCCACAACCGGAGCTAGCTACAATGCTCATGGATCGAATGGGAAGAAGTACTTGCTTCCTGCTCTGTTCGACCCTTCGACTTCAACTTGTTCTACTTTGGTTTGAGGGTTGA

Protein Analysis

320

Amino Acids

35.43

Weight (kDa)

9.3

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 37 - 235 2.8e-77 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35140 AT4G08950
fragaria_vesca FvH4_5g36900 FvH4_5g36910 FvH4_5g36920 FvH4_5g36930
malus_domestica MD08G1185900.v1.1 MD08G1186000.v1.1 MD08G1186100.v1.1 MD08G1186300.v1.1 MD15G1372200.v1.1 MD15G1372400.v1.1
prunus_persica Prupe.1G520600_v2.0.a1 Prupe.1G520700_v2.0.a1 Prupe.1G520800_v2.0.a1
pyrus_communis pycom08g15950 pycom08g15960 pycom08g15970 pycom08g16010 pycom15g33320 pycom15g33330
rosa_chinensis RchiOBHm_Chr0c28g0500911 RchiOBHm_Chr0c28g0500921 RchiOBHm_Chr0c28g0500931 RchiOBHm_Chr0c28g0500981 RchiOBHm_Chr0c28g0501021 RchiOBHm_Chr7g0243461 RchiOBHm_Chr7g0243471 RchiOBHm_Chr7g0243481 RchiOBHm_Chr7g0243521 RchiOBHm_Chr7g0243531 RchiOBHm_Chr7g0243541 RchiOBHm_Chr7g0243581 RchiOBHm_Chr7g0243621 RchiOBHm_Chr7g0243641 RchiOBHm_Chr7g0243671 RchiOBHm_Chr7g0243681 RchiOBHm_Chr7g0243691
rosa_laevigata RLG00000000840 RLG00000000841 RLG00000000842 RLG00000000843 RLG00000000844 RLG00000000845
rosa_multiflora Rmu_sc0000477.1_g000001 Rmu_sc0000477.1_g000003 Rmu_sc0001036.1_g000001 Rmu_sc0001036.1_g000002 Rmu_sc0004062.1_g000001 Rmu_sc0007755.1_g000001 Rmu_sc0008223.1_g000001 Rmu_sc0008473.1_g000006 Rmu_sc0009153.1_g000001 Rmu_sc0009153.1_g000002 Rmu_sc0029916.1_g000001
rosa_roxburghii Rroxscaffold_3G00222020 Rroxscaffold_3G00222030 Rroxscaffold_3G00222040 Rroxscaffold_3G00222060 Rroxscaffold_3G00222100
rosa_rugosa Rorug07G0319800.1 Rorug07G0319900 Rorug07G0320000 Rorug07G0320000 Rorug07G0320100 Rorug07G0320200 Rorug07G0320300
rosa_samantha Rh7BG445800 Rh7BG445900 Rh7BG446000 Rh7BG446100 Rh7BG446200 Rh7BG446300 Rh7BG446400 Rh7BG446500 Rh7CG491600 Rh7CG491700 Rh7CG491800 Rh7CG492000 Rh7CG492100 Rh7CG492200 Rh7CG492300 Rh7CG492500 Rh7CG492600 Rh7DG459900 Rh7DG460000 Rh7DG460100 Rh7DG460200 Rh7DG460300 Rh7DG460400 Rh7DG460600 Rh7DG460700
rosa_wichuraiana Rw7G039570 Rw7G039580 Rw7G039590 Rw7G039620 Rw7G039630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 2 cut(s) 258, 397
AccI GTMKAC 2 cut(s) 462, 800
AccII CGCG 3 cut(s) 290, 596, 721
AciI CCGC 6 cut(s) 169, 382, 423, 486, 712, 719
AclWI GGATC 2 cut(s) 535, 888
AcoI YGGCCR 2 cut(s) 166, 599
AcsI RAATTY 1 cut(s) 339
AcvI CACGTG 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 258
AfaI GTAC 3 cut(s) 113, 164, 898
AfiI CCNNNNNNNGG 8 cut(s) 258, 328, 397, 583, 620, 711, 843, 856
AgsI TTSAA 2 cut(s) 298, 932
AhlI ACTAGT 1 cut(s) 25
AjiI CACGTC 1 cut(s) 661
AjnI CCWGG 5 cut(s) 65, 666, 749, 792, 819
AluBI AGCT 6 cut(s) 8, 57, 397, 778, 862, 866
AluI AGCT 6 cut(s) 8, 57, 397, 778, 862, 866
Alw21I GWGCWC 1 cut(s) 415
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 2 cut(s) 535, 888
Ama87I CYCGRG 1 cut(s) 583
AoxI GGCC 7 cut(s) 121, 166, 399, 599, 645, 664, 812
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 3 cut(s) 5, 62, 778
ApoI RAATTY 1 cut(s) 339
AspLEI GCGC 2 cut(s) 292, 596
AspS9I GGNCC 9 cut(s) 122, 623, 646, 664, 716, 754, 812, 834, 846
AsuC2I CCSGG 2 cut(s) 584, 585
AsuHPI GGTGA 4 cut(s) 124, 166, 202, 691
AsuNHI GCTAGC 2 cut(s) 700, 862
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 5 cut(s) 623, 716, 754, 834, 846
BaeGI GKGCMC 2 cut(s) 415, 584
BalI TGGCCA 1 cut(s) 601
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BbrPI CACGTG 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 17, 74, 765
BccI CCATC 2 cut(s) 250, 623
BceAI ACGGC 6 cut(s) 136, 181, 511, 688, 756, 819
BciT130I CCWGG 5 cut(s) 67, 668, 751, 794, 821
BclI TGATCA 1 cut(s) 681
BcnI CCSGG 2 cut(s) 584, 585
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 4 cut(s) 26, 513, 701, 863
BglI GCCNNNNNGGC 1 cut(s) 672
BisI GCNGC 5 cut(s) 6, 63, 169, 487, 779
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 5 cut(s) 7, 64, 170, 488, 780
BmcAI AGTACT 1 cut(s) 898
Bme1390I CCNGG 7 cut(s) 67, 584, 585, 668, 751, 794, 821
Bme18I GGWCC 5 cut(s) 623, 716, 754, 834, 846
BmeT110I CYCGRG 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 9 cut(s) 122, 623, 646, 664, 716, 754, 812, 834, 846
BmrFI CCNGG 7 cut(s) 67, 584, 585, 668, 751, 794, 821
BmtI GCTAGC 2 cut(s) 704, 866
BoxI GACNNNNGTC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 58
BpuMI CCSGG 2 cut(s) 584, 585
BsaAI YACGTR 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 532
BsaJI CCNNGG 6 cut(s) 493, 583, 584, 750, 792, 793
BsaWI WCCGGW 2 cut(s) 134, 856
Bsc4I CCNNNNNNNGG 8 cut(s) 258, 328, 397, 583, 620, 711, 843, 856
Bse1I ACTGG 3 cut(s) 109, 577, 845
Bse8I GATNNNNATC 1 cut(s) 532
BseBI CCWGG 5 cut(s) 67, 668, 751, 794, 821
BseDI CCNNGG 6 cut(s) 493, 583, 584, 750, 792, 793
BseGI GGATG 2 cut(s) 242, 500
BseJI GATNNNNATC 1 cut(s) 532
BseLI CCNNNNNNNGG 8 cut(s) 258, 328, 397, 583, 620, 711, 843, 856
BseMII CTCAG 2 cut(s) 72, 582
BseNI ACTGG 3 cut(s) 109, 577, 845
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 2 cut(s) 415, 584
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 3 cut(s) 17, 74, 765
BseYI CCCAGC 2 cut(s) 183, 829
Bsh1236I CGCG 3 cut(s) 290, 596, 721
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 7 cut(s) 123, 168, 401, 601, 647, 666, 814
BshNI GGYRCC 1 cut(s) 488
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 415
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 3 cut(s) 135, 584, 857
BslFI GGGAC 2 cut(s) 729, 847
BslI CCNNNNNNNGG 8 cut(s) 258, 328, 397, 583, 620, 711, 843, 856
BsmFI GGGAC 2 cut(s) 729, 847
BsnI GGCC 7 cut(s) 123, 168, 401, 601, 647, 666, 814
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 3 cut(s) 415, 584, 635
Bsp143I GATC 4 cut(s) 156, 527, 681, 880
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 6 cut(s) 169, 382, 423, 486, 712, 719
BspANI GGCC 7 cut(s) 123, 168, 401, 601, 647, 666, 814
BspCNI CTCAG 2 cut(s) 71, 581
BspFNI CGCG 3 cut(s) 290, 596, 721
BspOI GCTAGC 2 cut(s) 704, 866
BspPI GGATC 2 cut(s) 535, 888
BspT107I GGYRCC 1 cut(s) 488
BsrI ACTGG 3 cut(s) 109, 577, 845
BssECI CCNNGG 6 cut(s) 493, 583, 584, 750, 792, 793
BssMI GATC 4 cut(s) 156, 527, 681, 880
Bst2UI CCWGG 5 cut(s) 67, 668, 751, 794, 821
Bst4CI ACNGT 1 cut(s) 482
Bst6I CTCTTC 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 255
BstC8I GCNNGC 5 cut(s) 189, 399, 702, 789, 864
BstDEI CTNAG 2 cut(s) 58, 568
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 2 cut(s) 242, 500
BstFNI CGCG 3 cut(s) 290, 596, 721
BstHHI GCGC 2 cut(s) 292, 596
BstKTI GATC 4 cut(s) 159, 530, 684, 883
BstMBI GATC 4 cut(s) 156, 527, 681, 880
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 5 cut(s) 11, 495, 672, 718, 784
BstNI CCWGG 5 cut(s) 67, 668, 751, 794, 821
BstPAI GACNNNNGTC 1 cut(s) 513
BstSCI CCNGG 7 cut(s) 65, 582, 583, 666, 749, 792, 819
BstSLI GKGCMC 2 cut(s) 415, 584
BstUI CGCG 3 cut(s) 290, 596, 721
BstV1I GCAGC 3 cut(s) 17, 74, 765
BstZI CGGCCG 1 cut(s) 166
BsuRI GGCC 7 cut(s) 123, 168, 401, 601, 647, 666, 814
BtgI CCRYGG 1 cut(s) 493
BtrI CACGTC 1 cut(s) 661
BtsCI GGATG 2 cut(s) 242, 500
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 2 cut(s) 584, 596
Cac8I GCNNGC 5 cut(s) 189, 399, 702, 789, 864
CfoI GCGC 2 cut(s) 292, 596
Cfr13I GGNCC 9 cut(s) 122, 623, 646, 664, 716, 754, 812, 834, 846
Cfr9I CCCGGG 1 cut(s) 583
CseI GACGC 1 cut(s) 518
Csp6I GTAC 3 cut(s) 112, 163, 897
CviAII CATG 3 cut(s) 475, 676, 877
CviQI GTAC 3 cut(s) 112, 163, 897
DdeI CTNAG 2 cut(s) 58, 568
DpnI GATC 4 cut(s) 158, 529, 683, 882
DpnII GATC 4 cut(s) 156, 527, 681, 880
DraIII CACNNNGTG 1 cut(s) 258
EaeI YGGCCR 2 cut(s) 166, 599
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
EclXI CGGCCG 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 635
Eco47I GGWCC 5 cut(s) 623, 716, 754, 834, 846
Eco52I CGGCCG 1 cut(s) 166
Eco72I CACGTG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 583
EcoO109I RGGNCCY 2 cut(s) 812, 834
EcoRII CCWGG 5 cut(s) 65, 666, 749, 792, 819
EcoT38I GRGCYC 1 cut(s) 635
FaeI CATG 3 cut(s) 478, 679, 880
FaiI YATR 6 cut(s) 269, 476, 621, 677, 827, 878
FalI AAGNNNNNCTT 2 cut(s) 883, 915
FaqI GGGAC 2 cut(s) 729, 847
FatI CATG 3 cut(s) 474, 675, 876
FauI CCCGC 2 cut(s) 389, 705
FbaI TGATCA 1 cut(s) 681
FblI GTMKAC 2 cut(s) 462, 800
Fnu4HI GCNGC 5 cut(s) 6, 63, 169, 487, 779
FokI GGATG 2 cut(s) 229, 487
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 5 cut(s) 6, 63, 169, 487, 779
FspBI CTAG 4 cut(s) 26, 513, 701, 863
GlaI GCGC 2 cut(s) 291, 595
GluI GCNGC 5 cut(s) 6, 63, 169, 487, 779
GsaI CCCAGC 2 cut(s) 187, 833
HaeIII GGCC 7 cut(s) 123, 168, 401, 601, 647, 666, 814
HapII CCGG 3 cut(s) 135, 584, 857
HgaI GACGC 1 cut(s) 518
HhaI GCGC 2 cut(s) 292, 596
Hin1II CATG 3 cut(s) 478, 679, 880
Hin6I GCGC 2 cut(s) 290, 594
HinP1I GCGC 2 cut(s) 290, 594
HincII GTYRAC 2 cut(s) 151, 463
HindII GTYRAC 2 cut(s) 151, 463
HpaII CCGG 3 cut(s) 135, 584, 857
HphI GGTGA 4 cut(s) 124, 166, 202, 691
Hpy166II GTNNAC 5 cut(s) 151, 413, 463, 801, 846
Hpy188I TCNGA 9 cut(s) 88, 202, 235, 451, 524, 563, 571, 736, 758
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 5 cut(s) 151, 413, 463, 801, 846
Hpy99I CGWCG 4 cut(s) 251, 467, 512, 662
HpyAV CCTTC 3 cut(s) 698, 753, 932
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 2 cut(s) 254, 660
HpyCH4V TGCA 5 cut(s) 22, 103, 191, 413, 474
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 495, 672, 718, 784
HpyF3I CTNAG 2 cut(s) 58, 568
HpySE526I ACGT 2 cut(s) 254, 660
Hsp92II CATG 3 cut(s) 478, 679, 880
HspAI GCGC 2 cut(s) 290, 594
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 4 cut(s) 156, 527, 681, 880
LmnI GCTCC 2 cut(s) 771, 859
Lsp1109I GCAGC 3 cut(s) 17, 74, 765
MaeI CTAG 4 cut(s) 26, 513, 701, 863
MaeII ACGT 2 cut(s) 254, 660
MalI GATC 4 cut(s) 158, 529, 683, 882
MboI GATC 4 cut(s) 156, 527, 681, 880
MboII GAAGA 3 cut(s) 199, 300, 903
MhlI GDGCHC 3 cut(s) 415, 584, 635
MlsI TGGCCA 1 cut(s) 601
MluCI AATT 4 cut(s) 104, 140, 339, 352
MluNI TGGCCA 1 cut(s) 601
MmeI TCCRAC 5 cut(s) 258, 415, 528, 541, 561
Mox20I TGGCCA 1 cut(s) 601
MscI TGGCCA 1 cut(s) 601
Msp20I TGGCCA 1 cut(s) 601
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 3 cut(s) 135, 584, 857
MspR9I CCNGG 7 cut(s) 67, 584, 585, 668, 751, 794, 821
MvaI CCWGG 5 cut(s) 67, 668, 751, 794, 821
MvnI CGCG 3 cut(s) 290, 596, 721
MwoI GCNNNNNNNGC 5 cut(s) 11, 495, 672, 718, 784
NciI CCSGG 2 cut(s) 584, 585
NdeII GATC 4 cut(s) 156, 527, 681, 880
NheI GCTAGC 2 cut(s) 700, 862
NlaIII CATG 3 cut(s) 478, 679, 880
PasI CCCWGGG 1 cut(s) 793
PflFI GACNNNGTC 1 cut(s) 445
PflMI CCANNNNNTGG 2 cut(s) 258, 397
PkrI GCNGC 5 cut(s) 7, 64, 170, 488, 780
PmaCI CACGTG 1 cut(s) 255
PmlI CACGTG 1 cut(s) 255
Ppu21I YACGTR 1 cut(s) 255
PpuMI RGGWCCY 1 cut(s) 834
PshAI GACNNNNGTC 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 834
Psp6I CCWGG 5 cut(s) 65, 666, 749, 792, 819
PspCI CACGTG 1 cut(s) 255
PspFI CCCAGC 2 cut(s) 183, 829
PspGI CCWGG 5 cut(s) 65, 666, 749, 792, 819
PspPI GGNCC 9 cut(s) 122, 623, 646, 664, 716, 754, 812, 834, 846
PspPPI RGGWCCY 1 cut(s) 834
PsyI GACNNNGTC 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 3 cut(s) 113, 164, 898
RsaNI GTAC 3 cut(s) 112, 163, 897
SalI GTCGAC 1 cut(s) 461
SatI GCNGC 5 cut(s) 6, 63, 169, 487, 779
Sau3AI GATC 4 cut(s) 156, 527, 681, 880
Sau96I GGNCC 9 cut(s) 122, 623, 646, 664, 716, 754, 812, 834, 846
ScaI AGTACT 1 cut(s) 898
ScrFI CCNGG 7 cut(s) 67, 584, 585, 668, 751, 794, 821
SduI GDGCHC 3 cut(s) 415, 584, 635
SinI GGWCC 5 cut(s) 623, 716, 754, 834, 846
SmaI CCCGGG 1 cut(s) 585
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 4 cut(s) 104, 140, 339, 352
SsiI CCGC 6 cut(s) 169, 382, 423, 486, 712, 719
SspMI CTAG 4 cut(s) 26, 513, 701, 863
StyD4I CCNGG 7 cut(s) 65, 582, 583, 666, 749, 792, 819
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 2 cut(s) 257, 663
TaqI TCGA 5 cut(s) 462, 691, 883, 917, 925
TasI AATT 4 cut(s) 104, 140, 339, 352
TatI WGTACW 1 cut(s) 896
TauI GCSGC 2 cut(s) 171, 489
TscAI CASTG 2 cut(s) 584, 596
TseI GCWGC 3 cut(s) 5, 62, 778
TspDTI ATGAA 4 cut(s) 38, 300, 363, 491
TspGWI ACGGA 2 cut(s) 136, 235
TspMI CCCGGG 1 cut(s) 583
TspRI CASTG 2 cut(s) 584, 596
Tth111I GACNNNGTC 1 cut(s) 445
Van91I CCANNNNNTGG 2 cut(s) 258, 397
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 5 cut(s) 623, 716, 754, 834, 846
XapI RAATTY 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 2 cut(s) 617, 658
XmaI CCCGGG 1 cut(s) 583
XmiI GTMKAC 2 cut(s) 462, 800
XspI CTAG 4 cut(s) 26, 513, 701, 863
ZrmI AGTACT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.