Rroxscaffold_3G00222040

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000422
Physical Location & Seq
Forward (+)
5044175 .. 5046678
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00222040.1

Sequence Viewer

Length: 981 bp
ATGGATGATCAGAATAAGCTGAACAGGAAGATACAGAGGAAGGAGAGTCTTGTCTCTCAAGGTCACCAAAAGAAAAAGATATCAAAGTCAAAACCCAAGAGCCTTGCTGAGACCTCAGACCAAACCCAGCAGCCTATACTATTCCAATACCACAATGGGCCTCTTCTCACTGGCAAAATCTCCATCAATTTGATCTGGTATGGGAAATTCAAGCCTTCCCAGCGCGCAATTGTTTCCGATTTCATCACCTCCCTTTCCACTTCTTCTCCATCTACAAACCAACCATCAGTTGCTACATGGTGGAAATCCATAGAGAAATACTACCACCTCTCCAATGCCAAGAAAACATCATCTCTTTCCCTATCACTGGGTACCCAAATCATCGACGAGAGCTGCTCTCTGGGCAAGTCCCTATCTGTTGGCAAACACATCAAACAGTTGGCAGCCAAGGGTGGCCAGAGTAATGCCATCAATGTTGTCTTGACCTCATCAGATGTTCTAATTGATGGGTTTTGCTCCAGCAAATGTGGTACACACGGGTCTTCAAAGAGCTCTTTCATCAGAGGCAAGAGCTCCAAGTTTGCTTACATCTGGGTCGGAAACTCCGAGACCCAATGCCCGGGTCAATGTGCTTGGCCATTCCATCAGCCAATTTACGGACCACAATCTCCACCTTTGGTTGCACCCAACAATGATGTGGGTTTGGACGGTATGATCATCAATATAGCTAGTCTTTTGGCGGGGACTGTGACCAACCCTTTTGGAAATGGGTTCTACCAGGGTCCCAAAGAGGCTCCATTAGAGGCTGCTTCGGCTTGTCCTGGTGTCTATGGGAAAGGAGCTTATCCTGGTTACGCCGGAGACCTCTTGGTTGATGCTACAACTGGTGCCAGCTACAATGCCAATGGTTTGAATGGGAAGAAGTACTTGCTTCCTGCTTTGTTTGACCCTTCAACCTCAACTTGTTCTACTCCTGTTTGA

Protein Analysis

326

Amino Acids

34.82

Weight (kDa)

9.34

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 49 - 325 6.9e-124 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35140 AT4G08950
fragaria_vesca FvH4_5g36900 FvH4_5g36910 FvH4_5g36920 FvH4_5g36930
malus_domestica MD08G1185900.v1.1 MD08G1186000.v1.1 MD08G1186100.v1.1 MD08G1186300.v1.1 MD15G1372200.v1.1 MD15G1372400.v1.1
prunus_persica Prupe.1G520600_v2.0.a1 Prupe.1G520700_v2.0.a1 Prupe.1G520800_v2.0.a1
pyrus_communis pycom08g15950 pycom08g15960 pycom08g15970 pycom08g16010 pycom15g33320 pycom15g33330
rosa_chinensis RchiOBHm_Chr0c28g0500911 RchiOBHm_Chr0c28g0500921 RchiOBHm_Chr0c28g0500931 RchiOBHm_Chr0c28g0500981 RchiOBHm_Chr0c28g0501021 RchiOBHm_Chr7g0243461 RchiOBHm_Chr7g0243471 RchiOBHm_Chr7g0243481 RchiOBHm_Chr7g0243521 RchiOBHm_Chr7g0243531 RchiOBHm_Chr7g0243541 RchiOBHm_Chr7g0243581 RchiOBHm_Chr7g0243621 RchiOBHm_Chr7g0243641 RchiOBHm_Chr7g0243671 RchiOBHm_Chr7g0243681 RchiOBHm_Chr7g0243691
rosa_laevigata RLG00000000840 RLG00000000841 RLG00000000842 RLG00000000843 RLG00000000844 RLG00000000845
rosa_multiflora Rmu_sc0000477.1_g000001 Rmu_sc0000477.1_g000003 Rmu_sc0001036.1_g000001 Rmu_sc0001036.1_g000002 Rmu_sc0004062.1_g000001 Rmu_sc0007755.1_g000001 Rmu_sc0008223.1_g000001 Rmu_sc0008473.1_g000006 Rmu_sc0009153.1_g000001 Rmu_sc0009153.1_g000002 Rmu_sc0029916.1_g000001
rosa_roxburghii Rroxscaffold_3G00222020 Rroxscaffold_3G00222030 Rroxscaffold_3G00222040 Rroxscaffold_3G00222060 Rroxscaffold_3G00222100
rosa_rugosa Rorug07G0319800.1 Rorug07G0319900 Rorug07G0320000 Rorug07G0320000 Rorug07G0320100 Rorug07G0320200 Rorug07G0320300
rosa_samantha Rh7BG445800 Rh7BG445900 Rh7BG446000 Rh7BG446100 Rh7BG446200 Rh7BG446300 Rh7BG446400 Rh7BG446500 Rh7CG491600 Rh7CG491700 Rh7CG491800 Rh7CG492000 Rh7CG492100 Rh7CG492200 Rh7CG492300 Rh7CG492500 Rh7CG492600 Rh7DG459900 Rh7DG460000 Rh7DG460100 Rh7DG460200 Rh7DG460300 Rh7DG460400 Rh7DG460600 Rh7DG460700
rosa_wichuraiana Rw7G039570 Rw7G039580 Rw7G039590 Rw7G039620 Rw7G039630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 2 cut(s) 371, 887
AccII CGCG 1 cut(s) 225
AciI CCGC 1 cut(s) 740
AcoI YGGCCR 2 cut(s) 454, 635
AcsI RAATTY 1 cut(s) 206
AfaI GTAC 3 cut(s) 373, 532, 926
AfiI CCNNNNNNNGG 3 cut(s) 367, 619, 656
AgsI TTSAA 4 cut(s) 211, 546, 913, 954
AjnI CCWGG 3 cut(s) 777, 820, 847
AluBI AGCT 7 cut(s) 19, 393, 552, 573, 728, 842, 894
AluI AGCT 7 cut(s) 19, 393, 552, 573, 728, 842, 894
Alw21I GWGCWC 2 cut(s) 554, 575
Alw26I GTCTC 4 cut(s) 58, 104, 602, 855
Ama87I CYCGRG 1 cut(s) 619
AoxI GGCC 3 cut(s) 158, 454, 635
ApeKI GCWGC 4 cut(s) 130, 393, 443, 806
ApoI RAATTY 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 371
AspLEI GCGC 2 cut(s) 225, 227
AspS9I GGNCC 3 cut(s) 158, 659, 782
AsuC2I CCSGG 2 cut(s) 620, 621
AsuHPI GGTGA 2 cut(s) 56, 238
AvaI CYCGRG 1 cut(s) 619
AvaII GGWCC 2 cut(s) 659, 782
BalI TGGCCA 2 cut(s) 456, 637
BanI GGYRCC 2 cut(s) 371, 887
BanII GRGCYC 2 cut(s) 554, 575
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 2 cut(s) 554, 575
BbvI GCAGC 4 cut(s) 142, 380, 455, 793
BccI CCATC 6 cut(s) 191, 277, 292, 476, 500, 651
BciT130I CCWGG 3 cut(s) 779, 822, 849
BclI TGATCA 2 cut(s) 7, 714
BcnI CCSGG 2 cut(s) 620, 621
BcoDI GTCTC 4 cut(s) 58, 104, 602, 855
BfaI CTAG 1 cut(s) 729
BisI GCNGC 4 cut(s) 131, 394, 444, 807
BlsI GCNGC 4 cut(s) 132, 395, 445, 808
BmcAI AGTACT 1 cut(s) 926
Bme1390I CCNGG 5 cut(s) 620, 621, 779, 822, 849
Bme18I GGWCC 2 cut(s) 659, 782
BmeT110I CYCGRG 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 158, 659, 782
BmiI GGNNCC 5 cut(s) 373, 783, 784, 795, 889
BmrFI CCNGG 5 cut(s) 620, 621, 779, 822, 849
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 865
BmuI ACTGGG 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 534
BplI GAGNNNNNCTC 4 cut(s) 380, 412, 382, 414
BpmI CTGGAG 1 cut(s) 502
BpuEI CTTGAG 1 cut(s) 42
BpuMI CCSGG 2 cut(s) 620, 621
BsaI GGTCTC 3 cut(s) 104, 602, 855
BsaJI CCNNGG 3 cut(s) 447, 619, 778
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 314, 344
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 619, 656
Bse1I ACTGG 3 cut(s) 175, 372, 889
BseBI CCWGG 3 cut(s) 779, 822, 849
BseDI CCNNGG 3 cut(s) 447, 619, 778
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 367, 619, 656
BseMII CTCAG 2 cut(s) 99, 129
BseNI ACTGG 3 cut(s) 175, 372, 889
BsePI GCGCGC 1 cut(s) 223
BseXI GCAGC 4 cut(s) 142, 380, 455, 793
BseYI CCCAGC 2 cut(s) 126, 219
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 160, 456, 637
BshNI GGYRCC 2 cut(s) 371, 887
BsiHKAI GWGCWC 2 cut(s) 554, 575
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 2 cut(s) 620, 858
BslFI GGGAC 3 cut(s) 394, 757, 768
BslI CCNNNNNNNGG 3 cut(s) 367, 619, 656
BsmAI GTCTC 4 cut(s) 58, 104, 602, 855
BsmFI GGGAC 3 cut(s) 394, 757, 768
BsnI GGCC 3 cut(s) 160, 456, 637
Bso31I GGTCTC 3 cut(s) 104, 602, 855
BsoBI CYCGRG 1 cut(s) 619
Bsp1286I GDGCHC 2 cut(s) 554, 575
Bsp143I GATC 3 cut(s) 7, 192, 714
BspACI CCGC 1 cut(s) 740
BspANI GGCC 3 cut(s) 160, 456, 637
BspCNI CTCAG 2 cut(s) 100, 128
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 5 cut(s) 373, 783, 784, 795, 889
BspT107I GGYRCC 2 cut(s) 371, 887
BspTNI GGTCTC 3 cut(s) 104, 602, 855
BsrI ACTGG 3 cut(s) 175, 372, 889
BssECI CCNNGG 3 cut(s) 447, 619, 778
BssHII GCGCGC 1 cut(s) 223
BssMI GATC 3 cut(s) 7, 192, 714
BssT1I CCWWGG 1 cut(s) 447
Bst2UI CCWGG 3 cut(s) 779, 822, 849
Bst4CI ACNGT 3 cut(s) 438, 710, 748
Bst6I CTCTTC 1 cut(s) 168
BstC8I GCNNGC 2 cut(s) 225, 892
BstDEI CTNAG 2 cut(s) 108, 115
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 2 cut(s) 225, 227
BstKTI GATC 3 cut(s) 10, 195, 717
BstMAI GTCTC 4 cut(s) 58, 104, 602, 855
BstMBI GATC 3 cut(s) 7, 192, 714
BstMWI GCNNNNNNNGC 3 cut(s) 220, 402, 812
BstNI CCWGG 3 cut(s) 779, 822, 849
BstPI GGTNACC 1 cut(s) 62
BstSCI CCNGG 5 cut(s) 618, 619, 777, 820, 847
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 4 cut(s) 142, 380, 455, 793
BstV2I GAAGAC 1 cut(s) 534
BsuRI GGCC 3 cut(s) 160, 456, 637
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 2 cut(s) 168, 365
Cac8I GCNNGC 2 cut(s) 225, 892
CfoI GCGC 2 cut(s) 225, 227
Cfr13I GGNCC 3 cut(s) 158, 659, 782
Cfr9I CCCGGG 1 cut(s) 619
Csp6I GTAC 3 cut(s) 372, 531, 925
CviAII CATG 1 cut(s) 297
CviQI GTAC 3 cut(s) 372, 531, 925
DdeI CTNAG 2 cut(s) 108, 115
DpnI GATC 3 cut(s) 9, 194, 716
DpnII GATC 3 cut(s) 7, 192, 714
EaeI YGGCCR 2 cut(s) 454, 635
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Ecl136II GAGCTC 2 cut(s) 552, 573
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 2 cut(s) 554, 575
Eco31I GGTCTC 3 cut(s) 104, 602, 855
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 659, 782
Eco53kI GAGCTC 2 cut(s) 552, 573
Eco88I CYCGRG 1 cut(s) 619
Eco91I GGTNACC 1 cut(s) 62
EcoICRI GAGCTC 2 cut(s) 552, 573
EcoO109I RGGNCCY 1 cut(s) 782
EcoO65I GGTNACC 1 cut(s) 62
EcoRII CCWGG 3 cut(s) 777, 820, 847
EcoRV GATATC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 2 cut(s) 554, 575
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 1 cut(s) 300
FaiI YATR 7 cut(s) 137, 201, 298, 311, 713, 725, 831
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FaqI GGGAC 3 cut(s) 394, 757, 768
FatI CATG 1 cut(s) 296
FauI CCCGC 1 cut(s) 733
FbaI TGATCA 2 cut(s) 7, 714
Fnu4HI GCNGC 4 cut(s) 131, 394, 444, 807
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 2 cut(s) 554, 575
Fsp4HI GCNGC 4 cut(s) 131, 394, 444, 807
FspBI CTAG 1 cut(s) 729
GlaI GCGC 2 cut(s) 224, 226
GluI GCNGC 4 cut(s) 131, 394, 444, 807
GsaI CCCAGC 2 cut(s) 130, 223
GsuI CTGGAG 1 cut(s) 502
HaeIII GGCC 3 cut(s) 160, 456, 637
HapII CCGG 2 cut(s) 620, 858
HhaI GCGC 2 cut(s) 225, 227
Hin1II CATG 1 cut(s) 300
Hin6I GCGC 2 cut(s) 223, 225
HinP1I GCGC 2 cut(s) 223, 225
HinfI GANTC 1 cut(s) 46
HpaII CCGG 2 cut(s) 620, 858
HphI GGTGA 2 cut(s) 56, 238
Hpy166II GTNNAC 1 cut(s) 533
Hpy188I TCNGA 7 cut(s) 12, 118, 238, 493, 563, 599, 607
Hpy188III TCNNGA 1 cut(s) 481
Hpy8I GTNNAC 1 cut(s) 533
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 3 cut(s) 34, 225, 960
HpyCH4III ACNGT 3 cut(s) 438, 710, 748
HpyCH4V TGCA 1 cut(s) 683
HpyF10VI GCNNNNNNNGC 3 cut(s) 220, 402, 812
HpyF3I CTNAG 2 cut(s) 108, 115
Hsp92II CATG 1 cut(s) 300
HspAI GCGC 2 cut(s) 223, 225
KflI GGGWCCC 1 cut(s) 782
KpnI GGTACC 1 cut(s) 375
Ksp22I TGATCA 2 cut(s) 7, 714
Kzo9I GATC 3 cut(s) 7, 192, 714
LmnI GCTCC 4 cut(s) 521, 578, 799, 839
Lsp1109I GCAGC 4 cut(s) 142, 380, 455, 793
LweI GCATC 1 cut(s) 865
MaeI CTAG 1 cut(s) 729
MaeIII GTNAC 3 cut(s) 62, 748, 851
MalI GATC 3 cut(s) 9, 194, 716
MboI GATC 3 cut(s) 7, 192, 714
MboII GAAGA 5 cut(s) 40, 155, 255, 534, 931
MfeI CAATTG 1 cut(s) 228
MhlI GDGCHC 2 cut(s) 554, 575
MlsI TGGCCA 2 cut(s) 456, 637
MluCI AATT 5 cut(s) 187, 206, 228, 501, 651
MluNI TGGCCA 2 cut(s) 456, 637
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 577
Mox20I TGGCCA 2 cut(s) 456, 637
MscI TGGCCA 2 cut(s) 456, 637
Msp20I TGGCCA 2 cut(s) 456, 637
MspI CCGG 2 cut(s) 620, 858
MspR9I CCNGG 5 cut(s) 620, 621, 779, 822, 849
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 3 cut(s) 779, 822, 849
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 3 cut(s) 220, 402, 812
NciI CCSGG 2 cut(s) 620, 621
NdeII GATC 3 cut(s) 7, 192, 714
NlaIII CATG 1 cut(s) 300
NlaIV GGNNCC 5 cut(s) 373, 783, 784, 795, 889
NmuCI GTSAC 2 cut(s) 62, 748
PauI GCGCGC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 603
PkrI GCNGC 4 cut(s) 132, 395, 445, 808
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 1 cut(s) 782
Psp124BI GAGCTC 2 cut(s) 554, 575
Psp5II RGGWCCY 1 cut(s) 782
Psp6I CCWGG 3 cut(s) 777, 820, 847
PspEI GGTNACC 1 cut(s) 62
PspFI CCCAGC 2 cut(s) 126, 219
PspGI CCWGG 3 cut(s) 777, 820, 847
PspN4I GGNNCC 5 cut(s) 373, 783, 784, 795, 889
PspPI GGNCC 3 cut(s) 158, 659, 782
PspPPI RGGWCCY 1 cut(s) 782
PteI GCGCGC 1 cut(s) 223
RsaI GTAC 3 cut(s) 373, 532, 926
RsaNI GTAC 3 cut(s) 372, 531, 925
SacI GAGCTC 2 cut(s) 554, 575
SatI GCNGC 4 cut(s) 131, 394, 444, 807
Sau3AI GATC 3 cut(s) 7, 192, 714
Sau96I GGNCC 3 cut(s) 158, 659, 782
ScaI AGTACT 1 cut(s) 926
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 5 cut(s) 620, 621, 779, 822, 849
SduI GDGCHC 2 cut(s) 554, 575
SfaNI GCATC 1 cut(s) 865
SinI GGWCC 2 cut(s) 659, 782
SmaI CCCGGG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 5 cut(s) 187, 206, 228, 501, 651
SsiI CCGC 1 cut(s) 740
SspMI CTAG 1 cut(s) 729
SstI GAGCTC 2 cut(s) 554, 575
StyD4I CCNGG 5 cut(s) 618, 619, 777, 820, 847
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 438, 710, 748
TaqI TCGA 1 cut(s) 384
TasI AATT 5 cut(s) 187, 206, 228, 501, 651
TatI WGTACW 1 cut(s) 924
TscAI CASTG 2 cut(s) 175, 372
TseFI GTSAC 2 cut(s) 62, 748
TseI GCWGC 4 cut(s) 130, 393, 443, 806
Tsp45I GTSAC 2 cut(s) 62, 748
TspDTI ATGAA 2 cut(s) 232, 547
TspGWI ACGGA 1 cut(s) 672
TspMI CCCGGG 1 cut(s) 619
TspRI CASTG 2 cut(s) 175, 372
VpaK11BI GGWCC 2 cut(s) 659, 782
XapI RAATTY 1 cut(s) 206
XcmI CCANNNNNNNNNTGG 2 cut(s) 152, 694
XmaI CCCGGG 1 cut(s) 619
XspI CTAG 1 cut(s) 729
ZrmI AGTACT 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.