MD15G1372400.v1.1

Phosphate-induced protein 1 conserved region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000422
Physical Location & Seq
Forward (+)
45395977 .. 45396921
945 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1372400.v1.1.491

Sequence Viewer

Length: 945 bp
ATGGCAGCTTTTGCAATTTTGAAACTAGTCCTTGTTATTTCTTTATTTCATTTCAGCTCAGCGGCCAGGAACCTTGCTAAAACTTCTGATAAAAACCAAATGCAATTCCAGTACCACAACGGCCCTCTCCTCACCGGAAAATTCTCCGTCAACTTGATTTGGTACGGCCGCTTCACCCCCTCCCAGCGTGCAATCGTTTCCGATTTCTTCACCTCCCTATCCAAACCCACCTCCGACCAACCATCTGTCGCCACGTGGTGGAAGTCCATAGATAAATACTACCACCTCGCGCATTTGAACACACCCTCTTCATCTCTCTCCCTTTCTCTGGGAACCCAAATTCTTGATGAAAATTACTCTCTAGGAAAATCTCTCACTTTCCGCCAAATCCAACAGCTGGCCTCAAAGGGTGCACAAAACTCCGCAATCAATGTCGTTTTGACATCGTCCGATGTTCTTATCGATGGGTTCTGCATGAACAGTTGCGGTACCCATGGCTCATTCAACGGCGCTAATCAAATCAGAGGATCAAAATCCAACAAGTTCACTTACATTTGGGTCGGAAACTCAGAGACCCAATGCCCAGGGCAGTGCGCGTGGCCATTCCACCAGCCCATCTATGGACCCCAGAGCCCACCCCTTGTGGCCCCTAACAACGACGTGGGCCTAGACGGCATGGTGATCAACCTGGCTAGCCTTTTGGCGGGGACCGCGACCAACCCCTTCAGAAACGGCTACTTCCAGGGTCCGAAAGAGGCTCCTTTGGAAGCTGCTTCATCTTGCCTTGGGGTATACGGTAAAGGGGCCTATCCTGGTTATGCTGGGGACCTTCTAGTTGACCCCACAACCGGAGCCAGCTACAATGCTCATGGATCGAATGGGAAGAAGTACTTGCTTCCTGCTTTGTTCGACCCTTCGACTTCAACTTGTTCTACTTTGGTTTGA

Protein Analysis

315

Amino Acids

33.79

Weight (kDa)

8.81

Isoelectric Point (pI)

22.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 37 - 313 6.6e-122 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000422)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35140 AT4G08950
fragaria_vesca FvH4_5g36900 FvH4_5g36910 FvH4_5g36920 FvH4_5g36930
malus_domestica MD08G1185900.v1.1 MD08G1186000.v1.1 MD08G1186100.v1.1 MD08G1186300.v1.1 MD15G1372200.v1.1 MD15G1372400.v1.1
prunus_persica Prupe.1G520600_v2.0.a1 Prupe.1G520700_v2.0.a1 Prupe.1G520800_v2.0.a1
pyrus_communis pycom08g15950 pycom08g15960 pycom08g15970 pycom08g16010 pycom15g33320 pycom15g33330
rosa_chinensis RchiOBHm_Chr0c28g0500911 RchiOBHm_Chr0c28g0500921 RchiOBHm_Chr0c28g0500931 RchiOBHm_Chr0c28g0500981 RchiOBHm_Chr0c28g0501021 RchiOBHm_Chr7g0243461 RchiOBHm_Chr7g0243471 RchiOBHm_Chr7g0243481 RchiOBHm_Chr7g0243521 RchiOBHm_Chr7g0243531 RchiOBHm_Chr7g0243541 RchiOBHm_Chr7g0243581 RchiOBHm_Chr7g0243621 RchiOBHm_Chr7g0243641 RchiOBHm_Chr7g0243671 RchiOBHm_Chr7g0243681 RchiOBHm_Chr7g0243691
rosa_laevigata RLG00000000840 RLG00000000841 RLG00000000842 RLG00000000843 RLG00000000844 RLG00000000845
rosa_multiflora Rmu_sc0000477.1_g000001 Rmu_sc0000477.1_g000003 Rmu_sc0001036.1_g000001 Rmu_sc0001036.1_g000002 Rmu_sc0004062.1_g000001 Rmu_sc0007755.1_g000001 Rmu_sc0008223.1_g000001 Rmu_sc0008473.1_g000006 Rmu_sc0009153.1_g000001 Rmu_sc0009153.1_g000002 Rmu_sc0029916.1_g000001
rosa_roxburghii Rroxscaffold_3G00222020 Rroxscaffold_3G00222030 Rroxscaffold_3G00222040 Rroxscaffold_3G00222060 Rroxscaffold_3G00222100
rosa_rugosa Rorug07G0319800.1 Rorug07G0319900 Rorug07G0320000 Rorug07G0320000 Rorug07G0320100 Rorug07G0320200 Rorug07G0320300
rosa_samantha Rh7BG445800 Rh7BG445900 Rh7BG446000 Rh7BG446100 Rh7BG446200 Rh7BG446300 Rh7BG446400 Rh7BG446500 Rh7CG491600 Rh7CG491700 Rh7CG491800 Rh7CG492000 Rh7CG492100 Rh7CG492200 Rh7CG492300 Rh7CG492500 Rh7CG492600 Rh7DG459900 Rh7DG460000 Rh7DG460100 Rh7DG460200 Rh7DG460300 Rh7DG460400 Rh7DG460600 Rh7DG460700
rosa_wichuraiana Rw7G039570 Rw7G039580 Rw7G039590 Rw7G039620 Rw7G039630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 2 cut(s) 258, 397
AccI GTMKAC 1 cut(s) 792
AccII CGCG 3 cut(s) 290, 596, 713
AciI CCGC 7 cut(s) 62, 169, 382, 423, 486, 704, 711
AclWI GGATC 2 cut(s) 535, 880
AcoI YGGCCR 3 cut(s) 63, 166, 599
AcsI RAATTY 2 cut(s) 140, 339
AcuI CTGAAG 1 cut(s) 709
AcvI CACGTG 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 258
AfaI GTAC 4 cut(s) 113, 164, 490, 890
AfiI CCNNNNNNNGG 6 cut(s) 258, 328, 397, 620, 703, 848
AgsI TTSAA 4 cut(s) 22, 298, 505, 924
AhlI ACTAGT 1 cut(s) 25
AjiI CACGTC 1 cut(s) 661
AjnI CCWGG 5 cut(s) 65, 583, 687, 741, 811
AluBI AGCT 5 cut(s) 8, 57, 397, 770, 858
AluI AGCT 5 cut(s) 8, 57, 397, 770, 858
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 1 cut(s) 566
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 2 cut(s) 535, 880
AoxI GGCC 8 cut(s) 63, 121, 166, 399, 599, 645, 664, 804
ApaLI GTGCAC 1 cut(s) 411
ApeKI GCWGC 2 cut(s) 5, 770
ApoI RAATTY 2 cut(s) 140, 339
Asp718I GGTACC 1 cut(s) 488
AspLEI GCGC 3 cut(s) 292, 512, 596
AspS9I GGNCC 8 cut(s) 122, 623, 646, 664, 708, 746, 804, 826
AsuHPI GGTGA 4 cut(s) 124, 166, 202, 691
AsuNHI GCTAGC 1 cut(s) 692
AvaII GGWCC 4 cut(s) 623, 708, 746, 826
BaeGI GKGCMC 1 cut(s) 415
BalI TGGCCA 1 cut(s) 601
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 635
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BbrPI CACGTG 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 17, 757
BccI CCATC 3 cut(s) 250, 458, 623
BceAI ACGGC 5 cut(s) 136, 181, 523, 688, 748
BciT130I CCWGG 5 cut(s) 67, 585, 689, 743, 813
BclI TGATCA 1 cut(s) 681
BcoDI GTCTC 1 cut(s) 566
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 5 cut(s) 26, 362, 668, 693, 833
BfoI RGCGCY 1 cut(s) 513
BglI GCCNNNNNGGC 1 cut(s) 672
BisI GCNGC 4 cut(s) 6, 63, 169, 771
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 4 cut(s) 7, 64, 170, 772
BmcAI AGTACT 1 cut(s) 890
Bme1390I CCNGG 5 cut(s) 67, 585, 689, 743, 813
Bme18I GGWCC 4 cut(s) 623, 708, 746, 826
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 8 cut(s) 122, 623, 646, 664, 708, 746, 804, 826
BmrFI CCNGG 5 cut(s) 67, 585, 689, 743, 813
BmtI GCTAGC 1 cut(s) 696
Bpu1102I GCTNAGC 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 532
BsaI GGTCTC 1 cut(s) 566
BsaJI CCNNGG 5 cut(s) 493, 583, 584, 742, 784
BsaWI WCCGGW 2 cut(s) 134, 848
Bsc4I CCNNNNNNNGG 6 cut(s) 258, 328, 397, 620, 703, 848
Bse1I ACTGG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 532
BseBI CCWGG 5 cut(s) 67, 585, 689, 743, 813
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 5 cut(s) 493, 583, 584, 742, 784
BseJI GATNNNNATC 1 cut(s) 532
BseLI CCNNNNNNNGG 6 cut(s) 258, 328, 397, 620, 703, 848
BseMII CTCAG 2 cut(s) 72, 582
BseNI ACTGG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 415
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 2 cut(s) 17, 757
BseYI CCCAGC 2 cut(s) 183, 821
Bsh1236I CGCG 3 cut(s) 290, 596, 713
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 8 cut(s) 65, 123, 168, 401, 601, 647, 666, 806
BshNI GGYRCC 1 cut(s) 488
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 2 cut(s) 135, 849
BslFI GGGAC 2 cut(s) 721, 839
BslI CCNNNNNNNGG 6 cut(s) 258, 328, 397, 620, 703, 848
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 2 cut(s) 721, 839
BsnI GGCC 8 cut(s) 65, 123, 168, 401, 601, 647, 666, 806
Bso31I GGTCTC 1 cut(s) 566
Bsp1286I GDGCHC 2 cut(s) 415, 635
Bsp143I GATC 3 cut(s) 527, 681, 872
Bsp1720I GCTNAGC 1 cut(s) 58
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 7 cut(s) 62, 169, 382, 423, 486, 704, 711
BspANI GGCC 8 cut(s) 65, 123, 168, 401, 601, 647, 666, 806
BspCNI CTCAG 2 cut(s) 71, 581
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 3 cut(s) 290, 596, 713
BspOI GCTAGC 1 cut(s) 696
BspPI GGATC 2 cut(s) 535, 880
BspT107I GGYRCC 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 566
BsrI ACTGG 1 cut(s) 109
BssECI CCNNGG 5 cut(s) 493, 583, 584, 742, 784
BssMI GATC 3 cut(s) 527, 681, 872
BssNAI GTATAC 1 cut(s) 793
BssT1I CCWWGG 2 cut(s) 493, 784
Bst1107I GTATAC 1 cut(s) 793
Bst2UI CCWGG 5 cut(s) 67, 585, 689, 743, 813
Bst4CI ACNGT 2 cut(s) 482, 797
Bst6I CTCTTC 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 255
BstC8I GCNNGC 4 cut(s) 189, 399, 694, 856
BstDEI CTNAG 2 cut(s) 58, 568
BstDSI CCRYGG 1 cut(s) 493
BstFNI CGCG 3 cut(s) 290, 596, 713
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 3 cut(s) 292, 512, 596
BstKTI GATC 3 cut(s) 530, 684, 875
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 3 cut(s) 527, 681, 872
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 11, 672, 710
BstNI CCWGG 5 cut(s) 67, 585, 689, 743, 813
BstSCI CCNGG 5 cut(s) 65, 583, 687, 741, 811
BstSLI GKGCMC 1 cut(s) 415
BstUI CGCG 3 cut(s) 290, 596, 713
BstV1I GCAGC 2 cut(s) 17, 757
BstZ17I GTATAC 1 cut(s) 793
BstZI CGGCCG 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 8 cut(s) 65, 123, 168, 401, 601, 647, 666, 806
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 493
BtrI CACGTC 1 cut(s) 661
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 1 cut(s) 596
Cac8I GCNNGC 4 cut(s) 189, 399, 694, 856
CfoI GCGC 3 cut(s) 292, 512, 596
Cfr13I GGNCC 8 cut(s) 122, 623, 646, 664, 708, 746, 804, 826
ClaI ATCGAT 1 cut(s) 462
Csp6I GTAC 4 cut(s) 112, 163, 489, 889
CviAII CATG 4 cut(s) 475, 494, 676, 869
CviQI GTAC 4 cut(s) 112, 163, 489, 889
DdeI CTNAG 2 cut(s) 58, 568
DpnI GATC 3 cut(s) 529, 683, 874
DpnII GATC 3 cut(s) 527, 681, 872
DraIII CACNNNGTG 1 cut(s) 258
EaeI YGGCCR 3 cut(s) 63, 166, 599
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
EciI GGCGGA 1 cut(s) 371
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 493, 784
Eco24I GRGCYC 1 cut(s) 635
Eco31I GGTCTC 1 cut(s) 566
Eco47I GGWCC 4 cut(s) 623, 708, 746, 826
Eco52I CGGCCG 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 709
Eco72I CACGTG 1 cut(s) 255
EcoO109I RGGNCCY 2 cut(s) 804, 826
EcoRII CCWGG 5 cut(s) 65, 583, 687, 741, 811
EcoT14I CCWWGG 2 cut(s) 493, 784
EcoT38I GRGCYC 1 cut(s) 635
ErhI CCWWGG 2 cut(s) 493, 784
FaeI CATG 4 cut(s) 478, 497, 679, 872
FaiI YATR 8 cut(s) 269, 476, 495, 621, 677, 793, 819, 870
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FaqI GGGAC 2 cut(s) 721, 839
FatI CATG 4 cut(s) 474, 493, 675, 868
FauI CCCGC 1 cut(s) 697
FbaI TGATCA 1 cut(s) 681
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 4 cut(s) 6, 63, 169, 771
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 4 cut(s) 6, 63, 169, 771
FspBI CTAG 5 cut(s) 26, 362, 668, 693, 833
GlaI GCGC 3 cut(s) 291, 511, 595
GluI GCNGC 4 cut(s) 6, 63, 169, 771
GsaI CCCAGC 2 cut(s) 187, 825
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 8 cut(s) 65, 123, 168, 401, 601, 647, 666, 806
HapII CCGG 2 cut(s) 135, 849
HhaI GCGC 3 cut(s) 292, 512, 596
Hin1II CATG 4 cut(s) 478, 497, 679, 872
Hin6I GCGC 3 cut(s) 290, 510, 594
HinP1I GCGC 3 cut(s) 290, 510, 594
HincII GTYRAC 2 cut(s) 151, 838
HindII GTYRAC 2 cut(s) 151, 838
HpaII CCGG 2 cut(s) 135, 849
HphI GGTGA 4 cut(s) 124, 166, 202, 691
Hpy166II GTNNAC 5 cut(s) 151, 413, 546, 793, 838
Hpy188I TCNGA 9 cut(s) 88, 202, 235, 451, 524, 563, 571, 728, 750
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 5 cut(s) 151, 413, 546, 793, 838
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 3 cut(s) 733, 839, 924
HpyCH4III ACNGT 2 cut(s) 482, 797
HpyCH4IV ACGT 2 cut(s) 254, 660
HpyCH4V TGCA 5 cut(s) 14, 103, 191, 413, 474
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 672, 710
HpyF3I CTNAG 2 cut(s) 58, 568
HpySE526I ACGT 2 cut(s) 254, 660
Hsp92II CATG 4 cut(s) 478, 497, 679, 872
HspAI GCGC 3 cut(s) 290, 510, 594
KpnI GGTACC 1 cut(s) 492
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 3 cut(s) 527, 681, 872
LmnI GCTCC 2 cut(s) 763, 851
Lsp1109I GCAGC 2 cut(s) 17, 757
MaeI CTAG 5 cut(s) 26, 362, 668, 693, 833
MaeII ACGT 2 cut(s) 254, 660
MalI GATC 3 cut(s) 529, 683, 874
MboI GATC 3 cut(s) 527, 681, 872
MboII GAAGA 3 cut(s) 199, 300, 895
MhlI GDGCHC 2 cut(s) 415, 635
MlsI TGGCCA 1 cut(s) 601
MluCI AATT 5 cut(s) 15, 104, 140, 339, 352
MluNI TGGCCA 1 cut(s) 601
MmeI TCCRAC 4 cut(s) 258, 415, 541, 561
Mox20I TGGCCA 1 cut(s) 601
MscI TGGCCA 1 cut(s) 601
Msp20I TGGCCA 1 cut(s) 601
MspA1I CMGCKG 2 cut(s) 62, 397
MspI CCGG 2 cut(s) 135, 849
MspR9I CCNGG 5 cut(s) 67, 585, 689, 743, 813
MvaI CCWGG 5 cut(s) 67, 585, 689, 743, 813
MvnI CGCG 3 cut(s) 290, 596, 713
MwoI GCNNNNNNNGC 3 cut(s) 11, 672, 710
NcoI CCATGG 1 cut(s) 493
NdeII GATC 3 cut(s) 527, 681, 872
NheI GCTAGC 1 cut(s) 692
NlaIII CATG 4 cut(s) 478, 497, 679, 872
PasI CCCWGGG 1 cut(s) 584
PflFI GACNNNGTC 1 cut(s) 445
PflMI CCANNNNNTGG 2 cut(s) 258, 397
PkrI GCNGC 4 cut(s) 7, 64, 170, 772
PmaCI CACGTG 1 cut(s) 255
PmlI CACGTG 1 cut(s) 255
Ppu21I YACGTR 1 cut(s) 255
PpuMI RGGWCCY 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 5 cut(s) 65, 583, 687, 741, 811
PspCI CACGTG 1 cut(s) 255
PspFI CCCAGC 2 cut(s) 183, 821
PspGI CCWGG 5 cut(s) 65, 583, 687, 741, 811
PspPI GGNCC 8 cut(s) 122, 623, 646, 664, 708, 746, 804, 826
PspPPI RGGWCCY 1 cut(s) 826
PsyI GACNNNGTC 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 4 cut(s) 113, 164, 490, 890
RsaNI GTAC 4 cut(s) 112, 163, 489, 889
SatI GCNGC 4 cut(s) 6, 63, 169, 771
Sau3AI GATC 3 cut(s) 527, 681, 872
Sau96I GGNCC 8 cut(s) 122, 623, 646, 664, 708, 746, 804, 826
ScaI AGTACT 1 cut(s) 890
ScrFI CCNGG 5 cut(s) 67, 585, 689, 743, 813
SduI GDGCHC 2 cut(s) 415, 635
SinI GGWCC 4 cut(s) 623, 708, 746, 826
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 5 cut(s) 15, 104, 140, 339, 352
SsiI CCGC 7 cut(s) 62, 169, 382, 423, 486, 704, 711
SspMI CTAG 5 cut(s) 26, 362, 668, 693, 833
StyD4I CCNGG 5 cut(s) 65, 583, 687, 741, 811
StyI CCWWGG 2 cut(s) 493, 784
TaaI ACNGT 2 cut(s) 482, 797
TaiI ACGT 2 cut(s) 257, 663
TaqI TCGA 4 cut(s) 462, 875, 909, 917
TasI AATT 5 cut(s) 15, 104, 140, 339, 352
TatI WGTACW 1 cut(s) 888
TauI GCSGC 2 cut(s) 65, 171
TscAI CASTG 1 cut(s) 596
TseI GCWGC 2 cut(s) 5, 770
TspDTI ATGAA 5 cut(s) 38, 300, 363, 491, 765
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 1 cut(s) 596
Tth111I GACNNNGTC 1 cut(s) 445
Van91I CCANNNNNTGG 2 cut(s) 258, 397
VneI GTGCAC 1 cut(s) 411
VpaK11BI GGWCC 4 cut(s) 623, 708, 746, 826
XapI RAATTY 2 cut(s) 140, 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 5 cut(s) 26, 362, 668, 693, 833
ZrmI AGTACT 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.