Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000299

Physical Location & Seq

Genomic Coordinates Reverse (-)

2359882 .. 2362272

Gene Structure 2391 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1026600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGGCTTTTCAACCACCATATCCTTCACCCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGTCCTTCTTATACAGATAATGCCCTTTCCCGCCACTGCTCAAACTCAGAAAATCATATCCTTGGGCACGTCCCTCACTGCAAAAGACGGAAACTCCTGGACCTCACCATCCGGCGACTTTGCTTTTGGTTTCCAAGAAATCGTTAAGCATGGATTCATACTTGCCATCTGGTTCAACAAAATACCAGAAAGAACTATTGTCTGGTCTGCCAACGGCGATAATCTAGTGAAGAAGGGATCCAAAGTTGAACTCACGGAAGATGGCAGGCTTATGCTCAAAGATACTGCAACAGTCACACCAATATGGACAGCTGATGCAGCTAGTGGAGTTGCCTATGCAGCTATGTTCGACACGGGAAATTTTGTGCTGGTCAACCAAGATTCAAAGAATTTGTGGGAGAGCTTTAATCAACCAACTGATACAATTCTACCCACGCAGATAATAAATCAAGGTAGCTCACTCTTCGCTCACCGCACACCATCAAATTATTCAAAAGGGAGGTTCCAGTTTACAGTGAAATCTGGCGGAGATCTCTCGCTTTATAATTTAAATTTCCCATTGGATTATGTTAATCTTGTTTATTGGCCGCCAACAGGCACCAAGTACAATGGTTTTCGGGTCACCTTCAACCAGTCTGGATCCATTTACCTTACAGCCCAGAACGGAAGCATAGTTTATATGATCTCAAACAATGCAGTTTCACTGGTAGACTACTATCAAAGGGCAACTCTCGAGTATAACGGAGTTTTCAGGCACTACGTGTACCCTAAAAGCATTAACTCGAACAGTGTGAACACGGAGCATGTGGCTTGGTCCTCGCTTTCCTTCATACCTTCAAATATCTGCACGTCCATCACGGGGTACACAGGCCCTAGTGCATGCGGGTTAAATAGCTTATGTAAAAATGATGAGGAAGGACCAGTTTGCTTGTGCCCGCATGGATACAGCTTTGTTGATCCAAGCGATGAGCTGGAAGGATGCAGACAGAACTTCGTTTCACAAAGTTGTGATGAAGCCTCGCCAGAAGATCATTTTTATTTTCAAGAAATGCAAAACAGCGATTGGACTACCGCATCTTATGAGTATTTCAATAATGTAAGTGAAGATTGGTGCAAGCAGAATTGCCTAAAGGACTGTTTCTGTGCCGCTCTCGTTTTCACAGAGGATGTCTGCTTGAAGAAGGGAACTCCTCTTTTCAACGGGAGGATTGACCCAACTCTCAGTTCTAAATCTCTAATCAAAATGAGGAAGAATTCTACAAATGAAAGAAAGCATGACGGTTCAACTTTCAAACGTGTCGAACCAGTTCTCCTAAGTAGTTCGGCTTTTCTGAACTTCTTCTTACTATTCATAACCTGCTTTCTTGTTTTGCGCTTTCTCAATGACAGAAAAGCAAATGCGAATATGTTCTACCCGGTCATTCAAGGGATTAATCTGAAATGTTTCTCCTACATGGAGTTAAAACAAGCTACCAACGGATTCATGGAAGAGCTAGGACGTGGTTCGTTTTCAACGGTTTTTAAAGGAGTTTTAGCGTCTGATAATGGAAAATGTGTTGCTGTTAAACGACTGGACACGATGATTGGAGGGAATGATTTGAAATTCAGAGCAGAAGTGAGCTCAATGGGCAGAACAAATCACAGAAATCTAGTCCAACTACTAGGGTTCTGCAATGAGAGGCATAACCGAATTCTTGTGTACGAGTTTATGAGCAATGGCTCCCTAGAAAGCTTTCTTTTTGGAGGGTCAAGGCCAAGTTGGTGCAAAAGAAAGGAAATTGCCTTGGGAACTGCAAGAGGGCTCTTGTATTTGCATGAGGAATGTAGCAGCCAAATCATTCATTGTGACATTAAGCCTCAAAACATTCTTCTAGACGACTCTTTCACAGCAAGAATTTCCGACTTTGGAGTGGCCAAGCTATTGATGATGGATCAAACTCGTACGGCTACTAAATTCCAGGGAACAAAAGGGTATGCAGCTCCTGAATGGTTCAAAAGCTTGCCTATCACAGTGAAGGCGGATGTTTACAGCTTTGGCATTTTGTTATTAGAGATTGTTTGCTGCAGGAAGCATTATGAAGCAAAGATAGAGGAACAAGAACAAATGATACTGGTGGATTGGGCGTATCATTGCTCTCAGAAAAGTAAACTGCACCGGCTGTTCGAGAATAATGAGGCAAAGGATGACCTGGAGGAGATGGAAATGTATTTGAAGATCGCATTTTGGTGCATTCAAGACGATCCAGCATTTCGACCAACCATGAAGAATGTCATACGAATGCTTGAAGGAAATGTTGAAGTCTTAACCCCCCCAAGTCCATCCTCATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase

Protein Analysis

797

Amino Acids

89.77

Weight (kDa)

6.31

Isoelectric Point (pI)

34.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	85 - 168	4.1e-15	D-mannose binding lectin
PK_Tyr_Ser-Thr	PF07714	514 - 781	4.2e-43	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	515 - 779	5.2e-48	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000299)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43401 FvH4_3g43402 FvH4_3g43403 FvH4_3g43440 FvH4_3g43710 FvH4_3g43772
malus_domestica	MD03G1021800.v1.1 MD03G1022000.v1.1 MD11G1024500.v1.1 MD11G1024700.v1.1 MD11G1025200.v1.1 MD11G1025500.v1.1 MD11G1025600.v1.1 MD11G1026200.v1.1 MD11G1026400.v1.1 MD11G1026500.v1.1 MD11G1026600.v1.1 MD11G1026800.v1.1 MD11G1027300.v1.1
prunus_persica	Prupe.6G020400_v2.0.a1 Prupe.6G020500_v2.0.a1 Prupe.6G020600_v2.0.a1 Prupe.6G020700_v2.0.a1 Prupe.6G020800_v2.0.a1 Prupe.6G020900_v2.0.a1 Prupe.6G021000_v2.0.a1 Prupe.6G021500_v2.0.a1 Prupe.6G021600_v2.0.a1
pyrus_communis	pycom03g01850 pycom03g01870 pycom03g01900 pycom03g01910 pycom11g01950 pycom11g01980 pycom11g01990 pycom11g02030 pycom11g02040
rosa_chinensis	RchiOBHm_Chr5g0077411 RchiOBHm_Chr5g0077421 RchiOBHm_Chr5g0077571 RchiOBHm_Chr5g0077601 RchiOBHm_Chr5g0077621 RchiOBHm_Chr5g0077651 RchiOBHm_Chr5g0077661 RchiOBHm_Chr5g0077671 RchiOBHm_Chr5g0077701 RchiOBHm_Chr5g0077741 RchiOBHm_Chr5g0077791 RchiOBHm_Chr5g0077861 RchiOBHm_Chr5g0078871 RchiOBHm_Chr5g0078901 RchiOBHm_Chr5g0078941 RchiOBHm_Chr5g0079081 RchiOBHm_Chr5g0079091 RchiOBHm_Chr7g0233481
rosa_laevigata	RLG00000036694 RLG00000036704 RLG00000036705 RLG00000036710 RLG00000036713 RLG00000036714 RLG00000036716 RLG00000036793 RLG00000036796 RLG00000036804 RLG00000036806
rosa_multiflora	Rmu_co8130992.1_g000001 Rmu_sc0000029.1_g000037 Rmu_sc0002187.1_g000023 Rmu_sc0004168.1_g000001 Rmu_sc0004168.1_g000004 Rmu_sc0004168.1_g000018 Rmu_sc0004168.1_g000028 Rmu_sc0004168.1_g000033 Rmu_sc0004168.1_g000056 Rmu_sc0004277.1_g000009 Rmu_sc0004277.1_g000016 Rmu_sc0004277.1_g000018 Rmu_sc0004277.1_g000020 Rmu_sc0004277.1_g000084 Rmu_sc0004277.1_g000087 Rmu_sc0012487.1_g000001 Rmu_sc0012487.1_g000002
rosa_roxburghii	Rroxscaffold_1G00003760 Rroxscaffold_1G00003780 Rroxscaffold_1G00004370 Rroxscaffold_1G00004400 Rroxscaffold_1G00004490 Rroxscaffold_1G00004510 Rroxscaffold_3G00227260 Rroxscaffold_5G00366480
rosa_rugosa	Rorug05G0454800 Rorug05G0455700 Rorug05G0455900 Rorug05G0456100 Rorug05G0456300 Rorug05G0456700.1 Rorug05G0461800 Rorug05G0462100 Rorug07G0280900.1 Rorug07G0281000
rosa_samantha	Rh5CG562200 Rh5DG543500 Rh5DG544200 Rh5DG544400 Rh5DG544500 Rh5DG544600 Rh5DG544700 Rh5DG544900 Rh5DG545200 Rh5DG549700 Rh7DG426000
rosa_wichuraiana	Rw0G003130 Rw0G023780 Rw0G023790 Rw5G046780 Rw5G047330 Rw5G047350 Rw5G047370 Rw5G047390 Rw5G047420 Rw7G036050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	603
Acc36I	ACCTGC	1 cut(s)	1424
AccB1I	GGYRCC	1 cut(s)	656
AccBSI	CCGCTC	1 cut(s)	1208
AccI	GTMKAC	1 cut(s)	768
AciI	CCGC	9 cut(s)	90, 532, 585, 647, 942, 995, 1131, 1206, 2078
AclWI	GGATC	7 cut(s)	291, 304, 693, 706, 1010, 1998, 2294
AcoI	YGGCCR	2 cut(s)	644, 1971
AcsI	RAATTY	8 cut(s)	418, 448, 610, 1312, 1661, 1749, 1953, 2012
AdeI	CACNNNGTG	1 cut(s)	820
AfaI	GTAC	5 cut(s)	665, 824, 923, 1760, 2002
AfiI	CCNNNNNNNGG	2 cut(s)	653, 918
AflIII	ACRYGT	2 cut(s)	819, 1354
AjiI	CACGTC	3 cut(s)	129, 909, 1559
AjnI	CCWGG	3 cut(s)	155, 2016, 2247
AjuI	GAANNNNNNNTTGG	2 cut(s)	1267, 1299
AloI	GAACNNNNNNTCC	2 cut(s)	1353, 1385
Alw21I	GWGCWC	1 cut(s)	1682
AlwI	GGATC	7 cut(s)	291, 304, 693, 706, 1010, 1998, 2294
AlwNI	CAGNNNCTG	1 cut(s)	2042
Ama87I	CYCGRG	1 cut(s)	791
AoxI	GGCC	4 cut(s)	644, 928, 1811, 1971
ApeKI	GCWGC	5 cut(s)	377, 398, 1887, 2036, 2121
ApoI	RAATTY	8 cut(s)	418, 448, 610, 1312, 1661, 1749, 1953, 2012
ArsI	GACNNNNNNTTYG	2 cut(s)	167, 199
AseI	ATTAAT	1 cut(s)	1491
Asp700I	GAANNNNTTC	5 cut(s)	1365, 1397, 1466, 1502, 1791
AspLEI	GCGC	1 cut(s)	1434
AspS9I	GGNCC	4 cut(s)	159, 873, 929, 977
AsuC2I	CCSGG	1 cut(s)	1475
AsuHPI	GGTGA	4 cut(s)	18, 156, 521, 673
AvaI	CYCGRG	1 cut(s)	791
AvaII	GGWCC	3 cut(s)	159, 873, 977
BaeGI	GKGCMC	2 cut(s)	128, 995
BalI	TGGCCA	1 cut(s)	1973
BamHI	GGATCC	2 cut(s)	296, 698
BanI	GGYRCC	1 cut(s)	656
BanII	GRGCYC	2 cut(s)	1682, 1863
Bbv12I	GWGCWC	1 cut(s)	1682
BbvI	GCAGC	5 cut(s)	389, 410, 1899, 2048, 2108
BccI	CCATC	8 cut(s)	175, 233, 314, 547, 920, 1981, 2251, 2386
BceAI	ACGGC	2 cut(s)	289, 2019
BciT130I	CCWGG	3 cut(s)	157, 2018, 2249
BciVI	GTATCC	1 cut(s)	995
BcnI	CCSGG	1 cut(s)	1475
BfaI	CTAG	8 cut(s)	284, 381, 933, 1553, 1709, 1721, 1784, 1931
BfmI	CTRYAG	1 cut(s)	2122
BfuAI	ACCTGC	1 cut(s)	1424
BfuI	GTATCC	1 cut(s)	995
BglII	AGATCT	1 cut(s)	589
BisI	GCNGC	7 cut(s)	378, 399, 647, 1206, 1888, 2037, 2122
BlsI	GCNGC	7 cut(s)	379, 400, 648, 1207, 1889, 2038, 2123
Bme1390I	CCNGG	4 cut(s)	157, 1475, 2018, 2249
Bme18I	GGWCC	3 cut(s)	159, 873, 977
BmeT110I	CYCGRG	1 cut(s)	791
BmgBI	CACGTC	3 cut(s)	129, 909, 1559
BmgT120I	GGNCC	4 cut(s)	159, 873, 929, 977
BmiI	GGNNCC	5 cut(s)	298, 563, 658, 700, 1780
BmrFI	CCNGG	4 cut(s)	157, 1475, 2018, 2249
BmsI	GCATC	3 cut(s)	364, 1028, 1142
BpmI	CTGGAG	1 cut(s)	2270
BpuMI	CCSGG	1 cut(s)	1475
BsaAI	YACGTR	1 cut(s)	820
BsaJI	CCNNGG	3 cut(s)	120, 1842, 2017
BsaXI	ACNNNNNCTCC	4 cut(s)	137, 167, 1353, 1383
Bsc4I	CCNNNNNNNGG	2 cut(s)	653, 918
Bse118I	RCCGGY	1 cut(s)	2214
Bse1I	ACTGG	7 cut(s)	565, 691, 768, 980, 1364, 1635, 2175
Bse3DI	GCAATG	3 cut(s)	1738, 1780, 2188
BseBI	CCWGG	3 cut(s)	157, 2018, 2249
BseDI	CCNNGG	3 cut(s)	120, 1842, 2017
BseGI	GGATG	6 cut(s)	167, 1043, 1231, 2086, 2248, 2378
BseLI	CCNNNNNNNGG	2 cut(s)	653, 918
BseMI	GCAATG	3 cut(s)	1738, 1780, 2188
BseMII	CTCAG	3 cut(s)	119, 1294, 2210
BseNI	ACTGG	7 cut(s)	565, 691, 768, 980, 1364, 1635, 2175
BseRI	GAGGAG	2 cut(s)	1239, 2267
BseSI	GKGCMC	2 cut(s)	128, 995
BseXI	GCAGC	5 cut(s)	389, 410, 1899, 2048, 2108
BsgI	GTGCAG	2 cut(s)	889, 2195
BshFI	GGCC	4 cut(s)	646, 930, 1813, 1973
BshNI	GGYRCC	1 cut(s)	656
BsiHKAI	GWGCWC	1 cut(s)	1682
BsiHKCI	CYCGRG	1 cut(s)	791
BsiSI	CCGG	3 cut(s)	171, 1475, 2215
BsiWI	CGTACG	1 cut(s)	2000
BslFI	GGGAC	1 cut(s)	115
BslI	CCNNNNNNNGG	2 cut(s)	653, 918
BsmFI	GGGAC	1 cut(s)	115
BsmI	GAATGC	2 cut(s)	2289, 2343
BsnI	GGCC	4 cut(s)	646, 930, 1813, 1973
BsoBI	CYCGRG	1 cut(s)	791
Bsp1286I	GDGCHC	4 cut(s)	128, 995, 1682, 1863
Bsp143I	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
BspACI	CCGC	9 cut(s)	90, 532, 585, 647, 942, 995, 1131, 1206, 2078
BspANI	GGCC	4 cut(s)	646, 930, 1813, 1973
BspCNI	CTCAG	3 cut(s)	118, 1293, 2209
BspLI	GGNNCC	5 cut(s)	298, 563, 658, 700, 1780
BspMAI	CTGCAG	1 cut(s)	2126
BspMI	ACCTGC	1 cut(s)	1424
BspPI	GGATC	7 cut(s)	291, 304, 693, 706, 1010, 1998, 2294
BspQI	GCTCTTC	1 cut(s)	1542
BspT107I	GGYRCC	1 cut(s)	656
BsrBI	CCGCTC	1 cut(s)	1208
BsrDI	GCAATG	3 cut(s)	1738, 1780, 2188
BsrFI	RCCGGY	1 cut(s)	2214
BsrI	ACTGG	7 cut(s)	565, 691, 768, 980, 1364, 1635, 2175
BssAI	RCCGGY	1 cut(s)	2214
BssECI	CCNNGG	3 cut(s)	120, 1842, 2017
BssMI	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
BssT1I	CCWWGG	2 cut(s)	120, 1842
Bst2UI	CCWGG	3 cut(s)	157, 2018, 2249
Bst4CI	ACNGT	7 cut(s)	352, 574, 848, 1196, 1340, 1576, 2071
Bst6I	CTCTTC	2 cut(s)	527, 1542
BstBAI	YACGTR	1 cut(s)	820
BstC8I	GCNNGC	5 cut(s)	326, 940, 995, 1175, 2060
BstDEI	CTNAG	4 cut(s)	105, 1280, 1373, 2196
BstEII	GGTNACC	1 cut(s)	679
BstF5I	GGATG	6 cut(s)	167, 1043, 1231, 2086, 2248, 2378
BstHHI	GCGC	1 cut(s)	1434
BstKTI	GATC	9 cut(s)	299, 592, 701, 744, 1018, 1090, 1993, 2277, 2302
BstMBI	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
BstMWI	GCNNNNNNNGC	3 cut(s)	377, 398, 1686
BstNI	CCWGG	3 cut(s)	157, 2018, 2249
BstNSI	RCATGY	2 cut(s)	866, 942
BstPI	GGTNACC	1 cut(s)	679
BstSCI	CCNGG	4 cut(s)	155, 1473, 2016, 2247
BstSFI	CTRYAG	1 cut(s)	2122
BstSLI	GKGCMC	2 cut(s)	128, 995
BstV1I	GCAGC	5 cut(s)	389, 410, 1899, 2048, 2108
BstX2I	RGATCY	3 cut(s)	296, 589, 698
BstYI	RGATCY	3 cut(s)	296, 589, 698
BsuI	GTATCC	1 cut(s)	995
BsuRI	GGCC	4 cut(s)	646, 930, 1813, 1973
BtgZI	GCGATG	1 cut(s)	1038
BtrI	CACGTC	3 cut(s)	129, 909, 1559
BtsCI	GGATG	6 cut(s)	167, 1043, 1231, 2086, 2248, 2378
BtsI	GCAGTG	2 cut(s)	93, 135
BtsIMutI	CAGTG	6 cut(s)	93, 135, 579, 761, 853, 2076
BveI	ACCTGC	1 cut(s)	1424
Cac8I	GCNNGC	5 cut(s)	326, 940, 995, 1175, 2060
CaiI	CAGNNNCTG	1 cut(s)	2042
CfoI	GCGC	1 cut(s)	1434
Cfr10I	RCCGGY	1 cut(s)	2214
Cfr13I	GGNCC	4 cut(s)	159, 873, 929, 977
CseI	GACGC	1 cut(s)	1584
Csp6I	GTAC	5 cut(s)	664, 823, 922, 1759, 2001
CviAII	CATG	9 cut(s)	209, 863, 939, 998, 1334, 1513, 1543, 1874, 2320
CviQI	GTAC	5 cut(s)	664, 823, 922, 1759, 2001
DdeI	CTNAG	4 cut(s)	105, 1280, 1373, 2196
DpnI	GATC	9 cut(s)	298, 591, 700, 743, 1017, 1089, 1992, 2276, 2301
DpnII	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
DraI	TTTAAA	2 cut(s)	609, 1582
DraIII	CACNNNGTG	1 cut(s)	820
EaeI	YGGCCR	2 cut(s)	644, 1971
Eam1104I	CTCTTC	2 cut(s)	527, 1542
EarI	CTCTTC	2 cut(s)	527, 1542
EciI	GGCGGA	2 cut(s)	600, 2093
Ecl136II	GAGCTC	1 cut(s)	1680
Eco130I	CCWWGG	2 cut(s)	120, 1842
Eco24I	GRGCYC	2 cut(s)	1682, 1863
Eco47I	GGWCC	3 cut(s)	159, 873, 977
Eco53kI	GAGCTC	1 cut(s)	1680
Eco88I	CYCGRG	1 cut(s)	791
Eco91I	GGTNACC	1 cut(s)	679
EcoICRI	GAGCTC	1 cut(s)	1680
EcoO109I	RGGNCCY	1 cut(s)	929
EcoO65I	GGTNACC	1 cut(s)	679
EcoRI	GAATTC	2 cut(s)	1312, 1749
EcoRII	CCWGG	3 cut(s)	155, 2016, 2247
EcoT14I	CCWWGG	2 cut(s)	120, 1842
EcoT38I	GRGCYC	2 cut(s)	1682, 1863
ErhI	CCWWGG	2 cut(s)	120, 1842
FaeI	CATG	9 cut(s)	212, 866, 942, 1001, 1337, 1516, 1546, 1877, 2323
FaqI	GGGAC	1 cut(s)	115
FatI	CATG	9 cut(s)	208, 862, 938, 997, 1333, 1512, 1542, 1873, 2319
FauI	CCCGC	3 cut(s)	97, 935, 1002
FblI	GTMKAC	1 cut(s)	768
Fnu4HI	GCNGC	7 cut(s)	378, 399, 647, 1206, 1888, 2037, 2122
FokI	GGATG	6 cut(s)	154, 1050, 1238, 2093, 2255, 2365
FriOI	GRGCYC	2 cut(s)	1682, 1863
Fsp4HI	GCNGC	7 cut(s)	378, 399, 647, 1206, 1888, 2037, 2122
FspBI	CTAG	8 cut(s)	284, 381, 933, 1553, 1709, 1721, 1784, 1931
GlaI	GCGC	1 cut(s)	1433
GluI	GCNGC	7 cut(s)	378, 399, 647, 1206, 1888, 2037, 2122
GsuI	CTGGAG	1 cut(s)	2270
HaeIII	GGCC	4 cut(s)	646, 930, 1813, 1973
HapII	CCGG	3 cut(s)	171, 1475, 2215
HgaI	GACGC	1 cut(s)	1584
HhaI	GCGC	1 cut(s)	1434
Hin1II	CATG	9 cut(s)	212, 866, 942, 1001, 1337, 1516, 1546, 1877, 2323
Hin6I	GCGC	1 cut(s)	1432
HinP1I	GCGC	1 cut(s)	1432
HincII	GTYRAC	1 cut(s)	433
HindII	GTYRAC	1 cut(s)	433
HindIII	AAGCTT	2 cut(s)	1789, 2056
HinfI	GANTC	4 cut(s)	213, 440, 1539, 1937
HpaII	CCGG	3 cut(s)	171, 1475, 2215
HphI	GGTGA	4 cut(s)	18, 156, 521, 673
Hpy166II	GTNNAC	9 cut(s)	433, 570, 769, 823, 853, 924, 1759, 2086, 2207
Hpy188I	TCNGA	7 cut(s)	108, 1392, 1497, 1600, 1667, 1960, 2199
Hpy188III	TCNNGA	7 cut(s)	696, 791, 1103, 1931, 2042, 2224, 2294
Hpy8I	GTNNAC	9 cut(s)	433, 570, 769, 823, 853, 924, 1759, 2086, 2207
HpyCH4III	ACNGT	7 cut(s)	352, 574, 848, 1196, 1340, 1576, 2071
HpyCH4IV	ACGT	5 cut(s)	128, 819, 908, 1354, 1558
HpyF10VI	GCNNNNNNNGC	3 cut(s)	377, 398, 1686
HpyF3I	CTNAG	4 cut(s)	105, 1280, 1373, 2196
HpySE526I	ACGT	5 cut(s)	128, 819, 908, 1354, 1558
Hsp92II	CATG	9 cut(s)	212, 866, 942, 1001, 1337, 1516, 1546, 1877, 2323
HspAI	GCGC	1 cut(s)	1432
Kzo9I	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
LguI	GCTCTTC	1 cut(s)	1542
LmnI	GCTCC	3 cut(s)	859, 1784, 2044
Lsp1109I	GCAGC	5 cut(s)	389, 410, 1899, 2048, 2108
LweI	GCATC	3 cut(s)	364, 1028, 1142
MaeI	CTAG	8 cut(s)	284, 381, 933, 1553, 1709, 1721, 1784, 1931
MaeII	ACGT	5 cut(s)	128, 819, 908, 1354, 1558
MaeIII	GTNAC	3 cut(s)	352, 679, 1904
MalI	GATC	9 cut(s)	298, 591, 700, 743, 1017, 1089, 1992, 2276, 2301
MbiI	CCGCTC	1 cut(s)	1208
MboI	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
MflI	RGATCY	3 cut(s)	296, 589, 698
MhlI	GDGCHC	4 cut(s)	128, 995, 1682, 1863
MlsI	TGGCCA	1 cut(s)	1973
MluNI	TGGCCA	1 cut(s)	1973
MlyI	GAGTC	1 cut(s)	1931
MmeI	TCCRAC	2 cut(s)	1738, 1983
Mox20I	TGGCCA	1 cut(s)	1973
MroXI	GAANNNNTTC	5 cut(s)	1365, 1397, 1466, 1502, 1791
MscI	TGGCCA	1 cut(s)	1973
MslI	CAYNNNNRTG	3 cut(s)	361, 2284, 2336
Msp20I	TGGCCA	1 cut(s)	1973
MspA1I	CMGCKG	1 cut(s)	371
MspI	CCGG	3 cut(s)	171, 1475, 2215
MspR9I	CCNGG	4 cut(s)	157, 1475, 2018, 2249
Mva1269I	GAATGC	2 cut(s)	2289, 2343
MvaI	CCWGG	3 cut(s)	157, 2018, 2249
MwoI	GCNNNNNNNGC	3 cut(s)	377, 398, 1686
NciI	CCSGG	1 cut(s)	1475
NdeII	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
NlaIII	CATG	9 cut(s)	212, 866, 942, 1001, 1337, 1516, 1546, 1877, 2323
NlaIV	GGNNCC	5 cut(s)	298, 563, 658, 700, 1780
NmuCI	GTSAC	3 cut(s)	352, 679, 1904
NspI	RCATGY	2 cut(s)	866, 942
PaeI	GCATGC	1 cut(s)	942
PaeR7I	CTCGAG	1 cut(s)	791
PciSI	GCTCTTC	1 cut(s)	1542
PctI	GAATGC	2 cut(s)	2289, 2343
PdmI	GAANNNNTTC	5 cut(s)	1365, 1397, 1466, 1502, 1791
PfeI	GAWTC	3 cut(s)	213, 440, 1539
Pfl23II	CGTACG	1 cut(s)	2000
PfoI	TCCNGGA	1 cut(s)	155
PkrI	GCNGC	7 cut(s)	379, 400, 648, 1207, 1889, 2038, 2123
PleI	GAGTC	1 cut(s)	1931
PpsI	GAGTC	1 cut(s)	1931
Ppu21I	YACGTR	1 cut(s)	820
PshBI	ATTAAT	1 cut(s)	1491
PsiI	TTATAA	1 cut(s)	603
Psp124BI	GAGCTC	1 cut(s)	1682
Psp6I	CCWGG	3 cut(s)	155, 2016, 2247
PspEI	GGTNACC	1 cut(s)	679
PspGI	CCWGG	3 cut(s)	155, 2016, 2247
PspLI	CGTACG	1 cut(s)	2000
PspN4I	GGNNCC	5 cut(s)	298, 563, 658, 700, 1780
PspPI	GGNCC	4 cut(s)	159, 873, 929, 977
PsrI	GAACNNNNNNTAC	2 cut(s)	2151, 2183
PstI	CTGCAG	1 cut(s)	2126
PstNI	CAGNNNCTG	1 cut(s)	2042
PsuI	RGATCY	3 cut(s)	296, 589, 698
PvuII	CAGCTG	1 cut(s)	371
RsaI	GTAC	5 cut(s)	665, 824, 923, 1760, 2002
RsaNI	GTAC	5 cut(s)	664, 823, 922, 1759, 2001
RseI	CAYNNNNRTG	3 cut(s)	361, 2284, 2336
SacI	GAGCTC	1 cut(s)	1682
SapI	GCTCTTC	1 cut(s)	1542
SatI	GCNGC	7 cut(s)	378, 399, 647, 1206, 1888, 2037, 2122
Sau3AI	GATC	9 cut(s)	296, 589, 698, 741, 1015, 1087, 1990, 2274, 2299
Sau96I	GGNCC	4 cut(s)	159, 873, 929, 977
SchI	GAGTC	1 cut(s)	1931
ScrFI	CCNGG	4 cut(s)	157, 1475, 2018, 2249
SduI	GDGCHC	4 cut(s)	128, 995, 1682, 1863
SfaNI	GCATC	3 cut(s)	364, 1028, 1142
SfcI	CTRYAG	1 cut(s)	2122
Sfr274I	CTCGAG	1 cut(s)	791
SinI	GGWCC	3 cut(s)	159, 873, 977
SlaI	CTCGAG	1 cut(s)	791
SmiI	ATTTAAAT	1 cut(s)	609
SmiMI	CAYNNNNRTG	3 cut(s)	361, 2284, 2336
SmlI	CTYRAG	1 cut(s)	791
SmoI	CTYRAG	1 cut(s)	791
SphI	GCATGC	1 cut(s)	942
SsiI	CCGC	9 cut(s)	90, 532, 585, 647, 942, 995, 1131, 1206, 2078
SspMI	CTAG	8 cut(s)	284, 381, 933, 1553, 1709, 1721, 1784, 1931
SstI	GAGCTC	1 cut(s)	1682
StyD4I	CCNGG	4 cut(s)	155, 1473, 2016, 2247
StyI	CCWWGG	2 cut(s)	120, 1842
SwaI	ATTTAAAT	1 cut(s)	609
TaaI	ACNGT	7 cut(s)	352, 574, 848, 1196, 1340, 1576, 2071
TaiI	ACGT	5 cut(s)	131, 822, 911, 1357, 1561
TaqI	TCGA	6 cut(s)	408, 792, 842, 1359, 2223, 2311
TatI	WGTACW	1 cut(s)	663
TauI	GCSGC	2 cut(s)	649, 1208
TfiI	GAWTC	3 cut(s)	213, 440, 1539
TscAI	CASTG	6 cut(s)	100, 142, 579, 768, 853, 2076
TseFI	GTSAC	3 cut(s)	352, 679, 1904
TseI	GCWGC	5 cut(s)	377, 398, 1887, 2036, 2121
Tsp45I	GTSAC	3 cut(s)	352, 679, 1904
TspDTI	ATGAA	9 cut(s)	205, 877, 1086, 1338, 1399, 1531, 1889, 2151, 2336
TspGWI	ACGGA	6 cut(s)	162, 329, 738, 816, 872, 1551
TspRI	CASTG	6 cut(s)	100, 142, 579, 768, 853, 2076
VpaK11BI	GGWCC	3 cut(s)	159, 873, 977
VspI	ATTAAT	1 cut(s)	1491
XapI	RAATTY	8 cut(s)	418, 448, 610, 1312, 1661, 1749, 1953, 2012
XbaI	TCTAGA	1 cut(s)	1930
XceI	RCATGY	2 cut(s)	866, 942
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1540
XhoI	CTCGAG	1 cut(s)	791
XmiI	GTMKAC	1 cut(s)	768
XmnI	GAANNNNTTC	5 cut(s)	1365, 1397, 1466, 1502, 1791
XspI	CTAG	8 cut(s)	284, 381, 933, 1553, 1709, 1721, 1784, 1931

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.