Rh5DG544700

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000299
Physical Location & Seq
Forward (+)
83822056 .. 83826240
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG544700.1

Sequence Viewer

Length: 1494 bp
ATGGCTTTTCATCTTCTATACCTTCTTTGCTTCATCCTTCTCGCGCTTCTCCCTTGTTCCACCATGGCTCAAACGTCGAAAAACATATCCTTAGGCTCATCCCTCACTGCACAAAACGACGATAACTCCTCCTGGCCATCCCCATCTGGTGAGTTTGCTTTTGGTTTTCGACAGATTGAGAAAGATGGTTTCTTACTAGCCATCTGGTTCGATAAAATACCAGAGAGAACAATCGTGTGGTCAGCAAATAGGGACAGCCTTGTGCCACAAGGATCAAAAGTTGAGCTCACCAGTGGTGGCCGATTTGCGCTCAATGATGCAAAGGGCAGTCAAATATGGTCTGCCAATTCTTCTAGTACCGGAGTTTCCTATGCAGCTATGCTCGACACCGGAAATTTTGTGCTGGCTAACCGAAACTCCACCTACTTGTGGGAGAGTTTTGATCACCCAACTGACACAATGCTTCCCACACAGAGTCTACAACAAAGTGGCAAACTCTTTGCTCGCTACGCGGTGACAAATTACTCGACAGGTAGATTCATGTTTTCACTACAACCTGATGGAAATCTCATGCTTTACACAACAAATTTCCCTCAAGATTCTGCCAATTTAACATACTGGTCAGTCAAAAATACTGAAATTGGATTTGAAGTCATCTTCAATCAGTCTGGCCTTATTTACCTTACCTCGGAGAATGGGACCATACTTGATACAATATCAGGCAAGCCCATTGCAACGCAAGATTTCTATCAGAGGGCAGCACTCGACTATGATGGAGTTTTGAGGCACTATGTTTACCCTAAAAGCAGCAGCACAAGCGGGGAAAGGTGGCCTATGGCTTGGTCTACTTCATCTTTCATACCGCCAAATATCTCTTTGTCAATTGTCGAAAAGGTAGGAATCGGTGCGTGTGGGTTCAACAGCTTGTATAGGATTGATAATACTTGTGAATGTCCAACTGGTTACAGTCCAATTGATCCAAATGATGAGCACAAAGGATGCAAACAGGACTTCGTTCCACAAAGCTGCAACGCACCGGCGGATGATTTTGACTTTCAAGAGGTGCCAAACACAAATTTTGCGGGTTTGGATTATGAACAATTTCAAGGGATGGCTGAGGATCGGTGCAGACAGAATTGCCTAAACGATTGCTTTTGTGCTGTTGCCATTTCCAATGCCGCCGGAGAGTGTTATATGAAGGGACTCCCTTTTGTGAATGGGTCGATTGATTCTAGTATTTATGGGACGAAAGCTCTTGTCAAATTTAGGAAACACAATTCTACTTCAAAAAAGAAAGATGATTCAACTTTGATCATTGTTGGATCAGTGCTCCTTAGTAGCTTGGGCATTCTGAACTTCATCTTACCTCTGATCACATATGTGGTTGTTTCTCGAATGTATTCTAGAAAAGCTGTGCCGGTTCATCCTAATTACCAAGGCATGAACTTGAAGTATTTCACTTATGAGGAGCTAAAAGATCGAAGCTACGAATGA

Protein Analysis

497

Amino Acids

55.26

Weight (kDa)

5.31

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 163 2.4e-20 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000299)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43401 FvH4_3g43402 FvH4_3g43403 FvH4_3g43440 FvH4_3g43710 FvH4_3g43772
malus_domestica MD03G1021800.v1.1 MD03G1022000.v1.1 MD11G1024500.v1.1 MD11G1024700.v1.1 MD11G1025200.v1.1 MD11G1025500.v1.1 MD11G1025600.v1.1 MD11G1026200.v1.1 MD11G1026400.v1.1 MD11G1026500.v1.1 MD11G1026600.v1.1 MD11G1026800.v1.1 MD11G1027300.v1.1
prunus_persica Prupe.6G020400_v2.0.a1 Prupe.6G020500_v2.0.a1 Prupe.6G020600_v2.0.a1 Prupe.6G020700_v2.0.a1 Prupe.6G020800_v2.0.a1 Prupe.6G020900_v2.0.a1 Prupe.6G021000_v2.0.a1 Prupe.6G021500_v2.0.a1 Prupe.6G021600_v2.0.a1
pyrus_communis pycom03g01850 pycom03g01870 pycom03g01900 pycom03g01910 pycom11g01950 pycom11g01980 pycom11g01990 pycom11g02030 pycom11g02040
rosa_chinensis RchiOBHm_Chr5g0077411 RchiOBHm_Chr5g0077421 RchiOBHm_Chr5g0077571 RchiOBHm_Chr5g0077601 RchiOBHm_Chr5g0077621 RchiOBHm_Chr5g0077651 RchiOBHm_Chr5g0077661 RchiOBHm_Chr5g0077671 RchiOBHm_Chr5g0077701 RchiOBHm_Chr5g0077741 RchiOBHm_Chr5g0077791 RchiOBHm_Chr5g0077861 RchiOBHm_Chr5g0078871 RchiOBHm_Chr5g0078901 RchiOBHm_Chr5g0078941 RchiOBHm_Chr5g0079081 RchiOBHm_Chr5g0079091 RchiOBHm_Chr7g0233481
rosa_laevigata RLG00000036694 RLG00000036704 RLG00000036705 RLG00000036710 RLG00000036713 RLG00000036714 RLG00000036716 RLG00000036793 RLG00000036796 RLG00000036804 RLG00000036806
rosa_multiflora Rmu_co8130992.1_g000001 Rmu_sc0000029.1_g000037 Rmu_sc0002187.1_g000023 Rmu_sc0004168.1_g000001 Rmu_sc0004168.1_g000004 Rmu_sc0004168.1_g000018 Rmu_sc0004168.1_g000028 Rmu_sc0004168.1_g000033 Rmu_sc0004168.1_g000056 Rmu_sc0004277.1_g000009 Rmu_sc0004277.1_g000016 Rmu_sc0004277.1_g000018 Rmu_sc0004277.1_g000020 Rmu_sc0004277.1_g000084 Rmu_sc0004277.1_g000087 Rmu_sc0012487.1_g000001 Rmu_sc0012487.1_g000002
rosa_roxburghii Rroxscaffold_1G00003760 Rroxscaffold_1G00003780 Rroxscaffold_1G00004370 Rroxscaffold_1G00004400 Rroxscaffold_1G00004490 Rroxscaffold_1G00004510 Rroxscaffold_3G00227260 Rroxscaffold_5G00366480
rosa_rugosa Rorug05G0454800 Rorug05G0455700 Rorug05G0455900 Rorug05G0456100 Rorug05G0456300 Rorug05G0456700.1 Rorug05G0461800 Rorug05G0462100 Rorug07G0280900.1 Rorug07G0281000
rosa_samantha Rh5CG562200 Rh5DG543500 Rh5DG544200 Rh5DG544400 Rh5DG544500 Rh5DG544600 Rh5DG544700 Rh5DG544900 Rh5DG545200 Rh5DG549700 Rh7DG426000
rosa_wichuraiana Rw0G003130 Rw0G023780 Rw0G023790 Rw5G046780 Rw5G047330 Rw5G047350 Rw5G047370 Rw5G047390 Rw5G047420 Rw7G036050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1063
AccI GTMKAC 2 cut(s) 478, 845
AccII CGCG 2 cut(s) 44, 512
AciI CCGC 6 cut(s) 512, 819, 863, 1040, 1082, 1179
AclWI GGATC 4 cut(s) 280, 971, 1128, 1330
AcoI YGGCCR 2 cut(s) 134, 298
AcsI RAATTY 4 cut(s) 394, 586, 1075, 1262
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 2 cut(s) 429, 688
AgsI TTSAA 8 cut(s) 650, 661, 919, 1058, 1106, 1287, 1305, 1450
AjnI CCWGG 1 cut(s) 131
AleI CACNNNNGTG 1 cut(s) 1379
AluBI AGCT 9 cut(s) 286, 377, 924, 1026, 1253, 1341, 1412, 1471, 1485
AluI AGCT 9 cut(s) 286, 377, 924, 1026, 1253, 1341, 1412, 1471, 1485
Alw21I GWGCWC 3 cut(s) 288, 993, 1332
AlwI GGATC 4 cut(s) 280, 971, 1128, 1330
AoxI GGCC 4 cut(s) 134, 298, 670, 830
ApeKI GCWGC 5 cut(s) 374, 758, 807, 810, 1026
ApoI RAATTY 4 cut(s) 394, 586, 1075, 1262
Asp700I GAANNNNTTC 3 cut(s) 1101, 1399, 1454
AspLEI GCGC 2 cut(s) 46, 310
AspS9I GGNCC 1 cut(s) 699
AsuHPI GGTGA 4 cut(s) 161, 280, 437, 526
AvaII GGWCC 1 cut(s) 699
AxyI CCTNAGG 1 cut(s) 91
BalI TGGCCA 1 cut(s) 136
BanI GGYRCC 1 cut(s) 1063
BanII GRGCYC 1 cut(s) 288
Bbv12I GWGCWC 3 cut(s) 288, 993, 1332
BbvCI CCTCAGC 1 cut(s) 1116
BbvI GCAGC 5 cut(s) 386, 770, 819, 822, 1013
BccI CCATC 7 cut(s) 145, 151, 179, 209, 554, 767, 1105
BciT130I CCWGG 1 cut(s) 133
BclI TGATCA 3 cut(s) 442, 1311, 1371
BfaI CTAG 4 cut(s) 197, 354, 1233, 1404
BisI GCNGC 6 cut(s) 375, 759, 808, 811, 1027, 1179
BlsI GCNGC 6 cut(s) 376, 760, 809, 812, 1028, 1180
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 699
BmgT120I GGNCC 1 cut(s) 699
BmiI GGNNCC 2 cut(s) 700, 1065
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 2 cut(s) 307, 989
Bpu10I CCTNAGC 1 cut(s) 1116
BpuEI CTTGAG 1 cut(s) 579
BsaBI GATNNNNATC 2 cut(s) 564, 747
BsaJI CCNNGG 3 cut(s) 63, 687, 1435
BsaWI WCCGGW 2 cut(s) 359, 389
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 688
Bse118I RCCGGY 2 cut(s) 1036, 1417
Bse1I ACTGG 3 cut(s) 291, 623, 964
Bse21I CCTNAGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 729
Bse8I GATNNNNATC 2 cut(s) 564, 747
BseBI CCWGG 1 cut(s) 133
BseDI CCNNGG 3 cut(s) 63, 687, 1435
BseGI GGATG 7 cut(s) 33, 98, 137, 1004, 1048, 1116, 1423
BseJI GATNNNNATC 2 cut(s) 564, 747
BseLI CCNNNNNNNGG 2 cut(s) 429, 688
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 1 cut(s) 1107
BseNI ACTGG 3 cut(s) 291, 623, 964
BseRI GAGGAG 2 cut(s) 118, 1481
BseXI GCAGC 5 cut(s) 386, 770, 819, 822, 1013
BsgI GTGCAG 2 cut(s) 93, 1147
Bsh1236I CGCG 2 cut(s) 44, 512
BshFI GGCC 4 cut(s) 136, 300, 672, 832
BshNI GGYRCC 1 cut(s) 1063
BsiHKAI GWGCWC 3 cut(s) 288, 993, 1332
BsiSI CCGG 5 cut(s) 360, 390, 1037, 1182, 1418
BslFI GGGAC 4 cut(s) 266, 712, 1215, 1258
BslI CCNNNNNNNGG 2 cut(s) 429, 688
BsmFI GGGAC 4 cut(s) 266, 712, 1215, 1258
BsmI GAATGC 1 cut(s) 1347
BsnI GGCC 4 cut(s) 136, 300, 672, 832
Bsp1286I GDGCHC 3 cut(s) 288, 993, 1332
Bsp143I GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 6 cut(s) 512, 819, 863, 1040, 1082, 1179
BspANI GGCC 4 cut(s) 136, 300, 672, 832
BspCNI CTCAG 1 cut(s) 1108
BspFNI CGCG 2 cut(s) 44, 512
BspLI GGNNCC 2 cut(s) 700, 1065
BspPI GGATC 4 cut(s) 280, 971, 1128, 1330
BspT107I GGYRCC 1 cut(s) 1063
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 2 cut(s) 1036, 1417
BsrI ACTGG 3 cut(s) 291, 623, 964
BssAI RCCGGY 2 cut(s) 1036, 1417
BssECI CCNNGG 3 cut(s) 63, 687, 1435
BssMI GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
BssT1I CCWWGG 2 cut(s) 63, 1435
Bst2UI CCWGG 1 cut(s) 133
Bst4CI ACNGT 1 cut(s) 968
BstC8I GCNNGC 3 cut(s) 405, 505, 725
BstDEI CTNAG 3 cut(s) 91, 1116, 1334
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 7 cut(s) 33, 98, 137, 1004, 1048, 1116, 1423
BstFNI CGCG 2 cut(s) 44, 512
BstHHI GCGC 2 cut(s) 46, 310
BstKTI GATC 8 cut(s) 275, 445, 979, 1123, 1314, 1325, 1374, 1480
BstMBI GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
BstMWI GCNNNNNNNGC 2 cut(s) 509, 816
BstNI CCWGG 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 131
BstUI CGCG 2 cut(s) 44, 512
BstV1I GCAGC 5 cut(s) 386, 770, 819, 822, 1013
Bsu36I CCTNAGG 1 cut(s) 91
BsuRI GGCC 4 cut(s) 136, 300, 672, 832
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 7 cut(s) 33, 98, 137, 1004, 1048, 1116, 1423
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 3 cut(s) 105, 298, 1332
Cac8I GCNNGC 3 cut(s) 405, 505, 725
CfoI GCGC 2 cut(s) 46, 310
Cfr10I RCCGGY 2 cut(s) 1036, 1417
Cfr13I GGNCC 1 cut(s) 699
Csp6I GTAC 1 cut(s) 357
CviAII CATG 4 cut(s) 64, 541, 571, 1441
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 3 cut(s) 91, 1116, 1334
DpnI GATC 8 cut(s) 274, 444, 978, 1122, 1313, 1324, 1373, 1479
DpnII GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
EaeI YGGCCR 2 cut(s) 134, 298
EciI GGCGGA 1 cut(s) 1055
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 63, 1435
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 286
Eco81I CCTNAGG 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 131
EcoT14I CCWWGG 2 cut(s) 63, 1435
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 2 cut(s) 63, 1435
FaeI CATG 4 cut(s) 67, 544, 574, 1444
FaqI GGGAC 4 cut(s) 266, 712, 1215, 1258
FatI CATG 4 cut(s) 63, 540, 570, 1440
FauI CCCGC 2 cut(s) 812, 1075
FauNDI CATATG 1 cut(s) 1378
FbaI TGATCA 3 cut(s) 442, 1311, 1371
FblI GTMKAC 2 cut(s) 478, 845
Fnu4HI GCNGC 6 cut(s) 375, 759, 808, 811, 1027, 1179
FokI GGATG 7 cut(s) 20, 85, 124, 1011, 1055, 1123, 1410
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 6 cut(s) 375, 759, 808, 811, 1027, 1179
FspBI CTAG 4 cut(s) 197, 354, 1233, 1404
GlaI GCGC 2 cut(s) 45, 309
GluI GCNGC 6 cut(s) 375, 759, 808, 811, 1027, 1179
HaeIII GGCC 4 cut(s) 136, 300, 672, 832
HapII CCGG 5 cut(s) 360, 390, 1037, 1182, 1418
HhaI GCGC 2 cut(s) 46, 310
Hin1II CATG 4 cut(s) 67, 544, 574, 1444
Hin6I GCGC 2 cut(s) 44, 308
HinP1I GCGC 2 cut(s) 44, 308
HinfI GANTC 7 cut(s) 475, 537, 599, 900, 1203, 1229, 1301
HpaII CCGG 5 cut(s) 360, 390, 1037, 1182, 1418
HphI GGTGA 4 cut(s) 161, 280, 437, 526
Hpy166II GTNNAC 3 cut(s) 479, 796, 846
Hpy188I TCNGA 4 cut(s) 691, 753, 1353, 1371
Hpy188III TCNNGA 4 cut(s) 596, 1058, 1392, 1404
Hpy8I GTNNAC 3 cut(s) 479, 796, 846
Hpy99I CGWCG 2 cut(s) 79, 122
HpyAV CCTTC 3 cut(s) 32, 47, 1192
HpyCH4III ACNGT 1 cut(s) 968
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 7 cut(s) 110, 320, 374, 734, 1002, 1029, 1128
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 816
HpyF3I CTNAG 3 cut(s) 91, 1116, 1334
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 4 cut(s) 67, 544, 574, 1444
HspAI GCGC 2 cut(s) 44, 308
Ksp22I TGATCA 3 cut(s) 442, 1311, 1371
Kzo9I GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
LmnI GCTCC 2 cut(s) 1335, 1468
Lsp1109I GCAGC 5 cut(s) 386, 770, 819, 822, 1013
LweI GCATC 2 cut(s) 307, 989
MaeI CTAG 4 cut(s) 197, 354, 1233, 1404
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 2 cut(s) 514, 962
MalI GATC 8 cut(s) 274, 444, 978, 1122, 1313, 1324, 1373, 1479
MboI GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
MboII GAAGA 3 cut(s) 5, 342, 649
MfeI CAATTG 2 cut(s) 882, 972
MhlI GDGCHC 3 cut(s) 288, 993, 1332
MlsI TGGCCA 1 cut(s) 136
MluNI TGGCCA 1 cut(s) 136
MlyI GAGTC 2 cut(s) 484, 1197
MmeI TCCRAC 2 cut(s) 980, 1300
Mox20I TGGCCA 1 cut(s) 136
MroXI GAANNNNTTC 3 cut(s) 1101, 1399, 1454
MscI TGGCCA 1 cut(s) 136
MseI TTAA 1 cut(s) 611
MslI CAYNNNNRTG 1 cut(s) 1379
Msp20I TGGCCA 1 cut(s) 136
MspI CCGG 5 cut(s) 360, 390, 1037, 1182, 1418
MspR9I CCNGG 1 cut(s) 133
MunI CAATTG 2 cut(s) 882, 972
Mva1269I GAATGC 1 cut(s) 1347
MvaI CCWGG 1 cut(s) 133
MvnI CGCG 2 cut(s) 44, 512
MwoI GCNNNNNNNGC 2 cut(s) 509, 816
NcoI CCATGG 1 cut(s) 63
NdeI CATATG 1 cut(s) 1378
NdeII GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
NlaIII CATG 4 cut(s) 67, 544, 574, 1444
NlaIV GGNNCC 2 cut(s) 700, 1065
NmuCI GTSAC 1 cut(s) 514
OliI CACNNNNGTG 1 cut(s) 1379
PctI GAATGC 1 cut(s) 1347
PdmI GAANNNNTTC 3 cut(s) 1101, 1399, 1454
PfeI GAWTC 5 cut(s) 537, 599, 900, 1229, 1301
PkrI GCNGC 6 cut(s) 376, 760, 809, 812, 1028, 1180
PleI GAGTC 2 cut(s) 483, 1197
PpsI GAGTC 2 cut(s) 483, 1197
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 131
PspGI CCWGG 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 700, 1065
PspPI GGNCC 1 cut(s) 699
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
RseI CAYNNNNRTG 1 cut(s) 1379
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 611
SatI GCNGC 6 cut(s) 375, 759, 808, 811, 1027, 1179
Sau3AI GATC 8 cut(s) 272, 442, 976, 1120, 1311, 1322, 1371, 1477
Sau96I GGNCC 1 cut(s) 699
SchI GAGTC 2 cut(s) 484, 1197
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 3 cut(s) 288, 993, 1332
SfaNI GCATC 2 cut(s) 307, 989
SgrAI CRCCGGYG 1 cut(s) 1036
SinI GGWCC 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 1379
SmlI CTYRAG 1 cut(s) 594
SmoI CTYRAG 1 cut(s) 594
SsiI CCGC 6 cut(s) 512, 819, 863, 1040, 1082, 1179
SspMI CTAG 4 cut(s) 197, 354, 1233, 1404
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 2 cut(s) 63, 1435
TaaI ACNGT 1 cut(s) 968
TaiI ACGT 1 cut(s) 77
TauI GCSGC 1 cut(s) 1181
TfiI GAWTC 5 cut(s) 537, 599, 900, 1229, 1301
Tru1I TTAA 1 cut(s) 611
Tru9I TTAA 1 cut(s) 611
TscAI CASTG 3 cut(s) 112, 298, 1332
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 5 cut(s) 374, 758, 807, 810, 1026
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 9 cut(s) 22, 529, 840, 847, 1110, 1211, 1348, 1412, 1457
TspRI CASTG 3 cut(s) 112, 298, 1332
VpaK11BI GGWCC 1 cut(s) 699
XapI RAATTY 4 cut(s) 394, 586, 1075, 1262
XbaI TCTAGA 1 cut(s) 1403
XmiI GTMKAC 2 cut(s) 478, 845
XmnI GAANNNNTTC 3 cut(s) 1101, 1399, 1454
XspI CTAG 4 cut(s) 197, 354, 1233, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.