Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000365

Physical Location & Seq

Genomic Coordinates Reverse (-)

902442 .. 911813

Gene Structure 9372 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1008900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGGAATCGAGTTGCACAGATTTAAACGGTGAAGGCTCCCTCGCCATGGTTCAGGCAGAACTAGACGCAGTACCTCTCAAATGCGCACCCATTGTGTCATCCTACAACGACAAGATCCGCCCTCTTCTGGATGCGGTAGACAAGTTACGCAGCTTGATGGTGATGGATGAAGGCATTCAGCTGCCAACGATTGTGGTGGTCGGAGACCAGTCATCGGGGAAGTCCAGCGTGCTCGAATCCTTGGCCGGCATCAGCCTCCCTCGGGGCCAAGGCATCTGCACGAGAGTGCCTCTTATTATGAGGCTGGAACACCACTCGAGTCCTCAGCCTGAACTTTCCCTGGAGTACAATGGCAGAGTTGATCGTACTGATGAAGACAATATCGCCAAAGACATTGTGAAAGCTACTATTTCTATTGCGGGAGGAGGTAAGGGAATTTCGAACACGCCACTTACTTTGTTGGTGAAGAAGAATGGGGTTCCGGATCTGACTATGGTTGATCTTCCGGGAATCACAAGGGTTCCGGTCCATGGCCAACCTGAAAATATTTATGATCAGATCAAAGATATGATCATGGAGTATATTAAGCCGGAAGAAAGTATTATTCTGAATGTGTTGTCTGCTACGGTGGATTTTACTACGTGTGAGTCAATTCGGATGTCACAAAGTGTAGATAAAACTGGTGAGAGGACGCTCGCTGTGGTTACGAAAGTGGACAAGGCACCGGAAGGACTACTTGAGAAGGTCACTGGAGATGATGTAAACATTGGTCTTGGTTATGTCTGTGTAAGGAACAGGATTGGTGACGAAACTTATGAGGAGGCAGCGGCGATGGCTCATGAACTATTTCAAACCCATCCCCTGCTTTCGAAGATTGACAAGTCCATTGTCGGTATTCCAGTTTTAGCACAGAAGCTGGTCCAAATTCAAGCAAACAGCATAGCAAGAAATATACCCGATATAGTGAAGAAGATCAATGACAGGCTGAACTTCTGCATTATGGAGCTGAACAAAATGCCAAAGAGTCTTTCATCTGTTGCCGAGGCAATGACAGCCTTTATGCAAATCATTGGCTTGGCTAAGGAATCGCTTAGGAAGATTTTTTTGAGAGGAGAATTTGAGGACTATGCGGACGACAATCGCATGCACTGCACTGCTAGATTGGTTGAGATGCTCAAACAGTACTCGGATGAACTTCACATATGTGCTGAAACTGGTGCAAAGAGCAACTTCTTGATGGAGGGGATCAAGATCTTGGAGGAGGCCAAAGGTATTTCACTTCCGAACTTCCTTCCACGCAATGCTTTCCTCATTATCTTGCAAGGAAAAGTGAATGAAATTTCGAGTATTCCGATAGCATTTGAGGAAAAAGTGTGGAGCTACATTGAAGAGGTGGTTATTTCTGTGTTGATGCATTATACGGAGAACTATCATCAGTTGCAGCTGTCAGCCAGGCGTGCCGGCCATAATCTAATGGCCAAGATGAAGGAAAGGTCAATCAAGTGGATGCTGGAGCTTGTAGAGATGGAAAAGCTAACGGATTATACATGTGATGATGATTATGTTTCCGAATGGAAGAAGTTGATGGCTAGTCAAAATCAATTCATTCATGAAGTCTTAACTGACGAGTATAAACCTTCTATCATAACTATAGAAGGCATCGGGGAAGTGGAAGTCGGTGTTCTGAGGATGTACCCGCATCCTTCTATCATAGATATAGAAGGCATCGGGGAAGTGGAAGTCGGTGTTCTGAGGAAGTACCCGCATGTGTTAGCTCAAGCTTATGACCTTAAAATGAGAATGATTGCTTATTGGAAGGTTGTTTTGAGGAGGCTTGTGGACTGCATGGCATTGCACTTGCAGCTGTCTGTTTTCAACCTTGTGAACAAAGAGATGGAAGCTGAGATTGTGAATGAGTTGTTGGGACCTTACGGTGGGGGAATTGAGAGGATGTTGGAGGAGTCCCCGGCAGTGGCAGTAAAGCGTGAGAAGCTTAACAAAAGCATAAAAAAACTGAGGGATTCTAAGGAGGTAGTGGCCAAGATTATGGACAGCATTGTCACTTATGGGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000266 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007005 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015631 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0044424 GO:0044464 GO:0048285 GO:0071840

KEGG Pathways

Metabolic & Signaling

ko04139 ko04214 ko04217 ko04621 ko04668 ko05162 ko05164 ko05165 map04139 map04214 map04217 map04621 map04668 map05162 map05164 map05165

Pfam Domains

Protein Families

Dynamin_M Dynamin_N GED

Protein Analysis

692

Amino Acids

77.18

Weight (kDa)

5.31

Isoelectric Point (pI)

40.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Dynamin_N	PF00350	64 - 238	5.4e-41	Dynamin family
Dynamin_M	PF01031	245 - 529	5.4e-58	Dynamin central region
GED	PF02212	593 - 681	2e-09	Dynamin GTPase effector domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60500 AT1G60530
fragaria_vesca	FvH4_4g34670 FvH4_7g03430 FvH4_7g03440 FvH4_7g03780
malus_domestica	MD02G1282600.v1.1 MD02G1282700.v1.1 MD02G1282900.v1.1 MD14G1008400.v1.1 MD14G1008800.v1.1 MD14G1008900.v1.1 MD14G1009000.v1.1 MD14G1009200.v1.1 MD14G1009300.v1.1
prunus_persica	Prupe.2G039500_v2.0.a1 Prupe.2G039600_v2.0.a1 Prupe.2G039800_v2.0.a1 Prupe.2G039900_v2.0.a1 Prupe.2G040000_v2.0.a1 Prupe.7G015100_v2.0.a1 Prupe.7G015100_v2.0.a1 Prupe.7G015300_v2.0.a1
pyrus_communis	pycom02g24110 pycom02g24120 pycom14g00720 pycom14g00740 pycom14g00780
rosa_chinensis	RchiOBHm_Chr1g0325781 RchiOBHm_Chr1g0326171 RchiOBHm_Chr1g0326211 RchiOBHm_Chr1g0327001 RchiOBHm_Chr1g0327051 RchiOBHm_Chr1g0327071 RchiOBHm_Chr1g0327091 RchiOBHm_Chr1g0327111 RchiOBHm_Chr2g0127731 RchiOBHm_Chr2g0127741 RchiOBHm_Chr4g0444021
rosa_laevigata	RLG00000005864 RLG00000005866 RLG00000030044 RLG00000030045 RLG00000030047 RLG00000030048 RLG00000030083 RLG00000030106
rosa_multiflora	Rmu_sc0000795.1_g000013 Rmu_sc0000795.1_g000046 Rmu_sc0000795.1_g000049 Rmu_sc0004240.1_g000005 Rmu_sc0004240.1_g000006 Rmu_sc0006009.1_g000005 Rmu_sc0015522.1_g000011 Rmu_sc0025852.1_g000001
rosa_roxburghii	Rroxscaffold_4G00323340 Rroxscaffold_4G00323420 Rroxscaffold_4G00323950 Rroxscaffold_4G00324390 Rroxscaffold_5G00384590
rosa_rugosa	Rorug01G0054400 Rorug01G0057700 Rorug01G0062800 Rorug01G0062900 Rorug01G0063200 Rorug01G0063300 Rorug04G0349000
rosa_samantha	Rh1AG069900 Rh1AG070200 Rh1AG070400 Rh1AG070700 Rh1AG071000 Rh1AG074600 Rh1AG079800 Rh1AG080000 Rh1AG080600 Rh1BG057600 Rh1BG060000 Rh1BG064000 Rh1CG070400 Rh1CG073100 Rh1CG077500 Rh1CG077600 Rh1CG078000 Rh1CG078600 Rh1DG075100 Rh1DG079900 Rh1DG084000 Rh1DG084100 Rh1DG084400 Rh1DG084900 Rh1DG085000 Rh2BG332700 Rh4AG409800 Rh4BG420900 Rh4CG435200 Rh4DG416100
rosa_wichuraiana	Rw1G005780 Rw1G005990 Rw1G006330 Rw1G006340 Rw1G006380 Rw1G006390 Rw2G026260 Rw4G035200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2057
Acc16I	TGCGCA	1 cut(s)	85
AccB1I	GGYRCC	1 cut(s)	721
AccI	GTMKAC	1 cut(s)	138
AccIII	TCCGGA	1 cut(s)	481
AciI	CCGC	7 cut(s)	118, 134, 419, 827, 1130, 1697, 1763
AclWI	GGATC	3 cut(s)	109, 492, 1253
AcoI	YGGCCR	5 cut(s)	243, 532, 1462, 1476, 2037
AcsI	RAATTY	4 cut(s)	435, 924, 1115, 1338
AdeI	CACNNNGTG	1 cut(s)	668
AfaI	GTAC	6 cut(s)	72, 347, 367, 1184, 1694, 1760
AfiI	CCNNNNNNNGG	7 cut(s)	46, 127, 214, 262, 530, 1934, 1972
AflIII	ACRYGT	2 cut(s)	641, 1547
AgsI	TTSAA	4 cut(s)	851, 929, 1388, 1876
AjnI	CCWGG	2 cut(s)	339, 1451
AjuI	GAANNNNNNNTTGG	4 cut(s)	1012, 1044, 1904, 1936
AleI	CACNNNNGTG	2 cut(s)	284, 1203
Alw21I	GWGCWC	1 cut(s)	234
Alw26I	GTCTC	1 cut(s)	198
AlwI	GGATC	3 cut(s)	109, 492, 1253
AlwNI	CAGNNNCTG	1 cut(s)	916
Ama87I	CYCGRG	2 cut(s)	261, 316
Aor13HI	TCCGGA	1 cut(s)	481
AoxI	GGCC	7 cut(s)	243, 265, 532, 1263, 1462, 1476, 2037
ApeKI	GCWGC	5 cut(s)	150, 181, 824, 1441, 1861
ApoI	RAATTY	4 cut(s)	435, 924, 1115, 1338
Asp700I	GAANNNNTTC	2 cut(s)	174, 846
AspLEI	GCGC	1 cut(s)	86
AspS9I	GGNCC	4 cut(s)	265, 526, 919, 1925
AsuC2I	CCSGG	2 cut(s)	507, 1967
AsuHPI	GGTGA	5 cut(s)	41, 172, 475, 695, 815
AsuII	TTCGAA	2 cut(s)	440, 869
AvaI	CYCGRG	2 cut(s)	261, 316
AvaII	GGWCC	3 cut(s)	526, 919, 1925
BalI	TGGCCA	3 cut(s)	534, 1478, 2039
BanI	GGYRCC	1 cut(s)	721
BauI	CACGAG	1 cut(s)	280
BbsI	GAAGAC	1 cut(s)	381
Bbv12I	GWGCWC	1 cut(s)	234
BbvCI	CCTCAGC	1 cut(s)	324
BbvI	GCAGC	5 cut(s)	162, 168, 836, 1453, 1873
BccI	CCATC	8 cut(s)	151, 157, 826, 864, 1231, 1519, 1579, 1888
BciT130I	CCWGG	2 cut(s)	341, 1453
BclI	TGATCA	2 cut(s)	553, 570
BcnI	CCSGG	2 cut(s)	507, 1967
BcoDI	GTCTC	1 cut(s)	198
BfaI	CTAG	3 cut(s)	62, 1158, 1590
BfmI	CTRYAG	1 cut(s)	1650
BglII	AGATCT	1 cut(s)	1251
BisI	GCNGC	6 cut(s)	151, 182, 825, 828, 1442, 1862
BlsI	GCNGC	6 cut(s)	152, 183, 826, 829, 1443, 1863
BmcAI	AGTACT	1 cut(s)	1184
Bme1390I	CCNGG	4 cut(s)	341, 507, 1453, 1967
Bme18I	GGWCC	3 cut(s)	526, 919, 1925
BmeT110I	CYCGRG	2 cut(s)	261, 316
BmgT120I	GGNCC	4 cut(s)	265, 526, 919, 1925
BmiI	GGNNCC	6 cut(s)	37, 266, 480, 522, 723, 1926
BmrFI	CCNGG	4 cut(s)	341, 507, 1453, 1967
BmsI	GCATC	9 cut(s)	121, 258, 282, 1161, 1401, 1497, 1668, 1708, 1734
BpiI	GAAGAC	1 cut(s)	381
BplI	GAGNNNNNCTC	2 cut(s)	274, 306
BpmI	CTGGAG	3 cut(s)	362, 771, 1532
Bpu10I	CCTNAGC	3 cut(s)	324, 1080, 1091
Bpu14I	TTCGAA	2 cut(s)	440, 869
BpuEI	CTTGAG	2 cut(s)	758, 1761
BpuMI	CCSGG	2 cut(s)	507, 1967
BsaAI	YACGTR	1 cut(s)	642
BsaBI	GATNNNNATC	1 cut(s)	1250
BsaI	GGTCTC	1 cut(s)	198
BsaJI	CCNNGG	8 cut(s)	45, 240, 260, 268, 339, 529, 1041, 1965
BsaWI	WCCGGW	3 cut(s)	481, 523, 724
BsaXI	ACNNNNNCTCC	2 cut(s)	744, 774
Bsc4I	CCNNNNNNNGG	7 cut(s)	46, 127, 214, 262, 530, 1934, 1972
Bse118I	RCCGGY	2 cut(s)	245, 1460
Bse1I	ACTGG	5 cut(s)	208, 685, 754, 899, 1219
Bse3DI	GCAATG	3 cut(s)	1053, 1306, 1850
Bse8I	GATNNNNATC	1 cut(s)	1250
BseAI	TCCGGA	1 cut(s)	481
BseBI	CCWGG	2 cut(s)	341, 1453
BseDI	CCNNGG	8 cut(s)	45, 240, 260, 268, 339, 529, 1041, 1965
BseJI	GATNNNNATC	1 cut(s)	1250
BseLI	CCNNNNNNNGG	7 cut(s)	46, 127, 214, 262, 530, 1934, 1972
BseMI	GCAATG	3 cut(s)	1053, 1306, 1850
BseMII	CTCAG	5 cut(s)	338, 1676, 1742, 1893, 2006
BseNI	ACTGG	5 cut(s)	208, 685, 754, 899, 1219
BseRI	GAGGAG	6 cut(s)	438, 833, 1125, 1274, 1843, 1973
BseXI	GCAGC	5 cut(s)	162, 168, 836, 1453, 1873
BsgI	GTGCAG	2 cut(s)	262, 1135
BshFI	GGCC	7 cut(s)	245, 267, 534, 1265, 1464, 1478, 2039
BshNI	GGYRCC	1 cut(s)	721
BsiHKAI	GWGCWC	1 cut(s)	234
BsiHKCI	CYCGRG	2 cut(s)	261, 316
BsiSI	CCGG	8 cut(s)	246, 482, 506, 524, 590, 725, 1461, 1967
BslFI	GGGAC	2 cut(s)	1938, 1948
BslI	CCNNNNNNNGG	7 cut(s)	46, 127, 214, 262, 530, 1934, 1972
BsmAI	GTCTC	1 cut(s)	198
BsmFI	GGGAC	2 cut(s)	1938, 1948
BsmI	GAATGC	1 cut(s)	174
BsnI	GGCC	7 cut(s)	245, 267, 534, 1265, 1464, 1478, 2039
Bso31I	GGTCTC	1 cut(s)	198
BsoBI	CYCGRG	2 cut(s)	261, 316
Bsp119I	TTCGAA	2 cut(s)	440, 869
Bsp1286I	GDGCHC	1 cut(s)	234
Bsp13I	TCCGGA	1 cut(s)	481
Bsp19I	CCATGG	2 cut(s)	45, 529
BspACI	CCGC	7 cut(s)	118, 134, 419, 827, 1130, 1697, 1763
BspANI	GGCC	7 cut(s)	245, 267, 534, 1265, 1464, 1478, 2039
BspCNI	CTCAG	5 cut(s)	337, 1677, 1743, 1894, 2007
BspEI	TCCGGA	1 cut(s)	481
BspHI	TCATGA	2 cut(s)	838, 1609
BspLI	GGNNCC	6 cut(s)	37, 266, 480, 522, 723, 1926
BspPI	GGATC	3 cut(s)	109, 492, 1253
BspT104I	TTCGAA	2 cut(s)	440, 869
BspT107I	GGYRCC	1 cut(s)	721
BspTNI	GGTCTC	1 cut(s)	198
BsrDI	GCAATG	3 cut(s)	1053, 1306, 1850
BsrFI	RCCGGY	2 cut(s)	245, 1460
BsrI	ACTGG	5 cut(s)	208, 685, 754, 899, 1219
BssAI	RCCGGY	2 cut(s)	245, 1460
BssECI	CCNNGG	8 cut(s)	45, 240, 260, 268, 339, 529, 1041, 1965
BssSI	CACGAG	1 cut(s)	280
BssT1I	CCWWGG	4 cut(s)	45, 240, 268, 529
Bst2BI	CACGAG	1 cut(s)	280
Bst2UI	CCWGG	2 cut(s)	341, 1453
Bst4CI	ACNGT	4 cut(s)	29, 628, 1182, 1934
Bst6I	CTCTTC	2 cut(s)	129, 1383
BstAPI	GCANNNNNTGC	1 cut(s)	1149
BstBAI	YACGTR	1 cut(s)	642
BstBI	TTCGAA	2 cut(s)	440, 869
BstC8I	GCNNGC	6 cut(s)	230, 247, 696, 1145, 1458, 1462
BstDEI	CTNAG	8 cut(s)	324, 1080, 1091, 1685, 1751, 1902, 2015, 2025
BstDSI	CCRYGG	2 cut(s)	45, 529
BstHHI	GCGC	1 cut(s)	86
BstMAI	GTCTC	1 cut(s)	198
BstMWI	GCNNNNNNNGC	6 cut(s)	833, 1052, 1149, 1457, 1861, 1990
BstNI	CCWGG	2 cut(s)	341, 1453
BstNSI	RCATGY	3 cut(s)	1147, 1551, 1769
BstSCI	CCNGG	4 cut(s)	339, 505, 1451, 1965
BstSFI	CTRYAG	1 cut(s)	1650
BstV1I	GCAGC	5 cut(s)	162, 168, 836, 1453, 1873
BstV2I	GAAGAC	1 cut(s)	381
BstX2I	RGATCY	3 cut(s)	114, 484, 1251
BstXI	CCANNNNNNTGG	1 cut(s)	2047
BstYI	RGATCY	3 cut(s)	114, 484, 1251
BsuRI	GGCC	7 cut(s)	245, 267, 534, 1265, 1464, 1478, 2039
BtgI	CCRYGG	2 cut(s)	45, 529
BtgZI	GCGATG	1 cut(s)	845
BtsI	GCAGTG	3 cut(s)	1147, 1152, 1977
BtsIMutI	CAGTG	4 cut(s)	747, 1147, 1152, 1977
Cac8I	GCNNGC	6 cut(s)	230, 247, 696, 1145, 1458, 1462
CaiI	CAGNNNCTG	1 cut(s)	916
CciI	TCATGA	2 cut(s)	838, 1609
CfoI	GCGC	1 cut(s)	86
Cfr10I	RCCGGY	2 cut(s)	245, 1460
Cfr13I	GGNCC	4 cut(s)	265, 526, 919, 1925
CseI	GACGC	2 cut(s)	74, 700
Csp6I	GTAC	6 cut(s)	71, 346, 366, 1183, 1693, 1759
CspCI	CAANNNNNGTGG	4 cut(s)	174, 209, 438, 473
CviAII	CATG	9 cut(s)	46, 530, 574, 839, 1144, 1548, 1610, 1766, 1846
CviQI	GTAC	6 cut(s)	71, 346, 366, 1183, 1693, 1759
DdeI	CTNAG	8 cut(s)	324, 1080, 1091, 1685, 1751, 1902, 2015, 2025
DraI	TTTAAA	1 cut(s)	24
DraIII	CACNNNGTG	1 cut(s)	668
DrdI	GACNNNNNNGTC	1 cut(s)	2057
DseDI	GACNNNNNNGTC	1 cut(s)	2057
EaeI	YGGCCR	5 cut(s)	243, 532, 1462, 1476, 2037
Eam1104I	CTCTTC	2 cut(s)	129, 1383
EarI	CTCTTC	2 cut(s)	129, 1383
EciI	GGCGGA	1 cut(s)	107
Eco130I	CCWWGG	4 cut(s)	45, 240, 268, 529
Eco31I	GGTCTC	1 cut(s)	198
Eco47I	GGWCC	3 cut(s)	526, 919, 1925
Eco88I	CYCGRG	2 cut(s)	261, 316
EcoO109I	RGGNCCY	1 cut(s)	1925
EcoRII	CCWGG	2 cut(s)	339, 1451
EcoT14I	CCWWGG	4 cut(s)	45, 240, 268, 529
EcoT22I	ATGCAT	1 cut(s)	1416
ErhI	CCWWGG	4 cut(s)	45, 240, 268, 529
FaeI	CATG	9 cut(s)	49, 533, 577, 842, 1147, 1551, 1613, 1769, 1849
FalI	AAGNNNNNCTT	4 cut(s)	720, 752, 1214, 1246
FaqI	GGGAC	2 cut(s)	1938, 1948
FatI	CATG	9 cut(s)	45, 529, 573, 838, 1143, 1547, 1609, 1765, 1845
FauI	CCCGC	3 cut(s)	412, 1704, 1770
FauNDI	CATATG	1 cut(s)	1202
FbaI	TGATCA	2 cut(s)	553, 570
FblI	GTMKAC	1 cut(s)	138
Fnu4HI	GCNGC	6 cut(s)	151, 182, 825, 828, 1442, 1862
Fsp4HI	GCNGC	6 cut(s)	151, 182, 825, 828, 1442, 1862
FspAI	RTGCGCAY	1 cut(s)	85
FspBI	CTAG	3 cut(s)	62, 1158, 1590
FspI	TGCGCA	1 cut(s)	85
GlaI	GCGC	1 cut(s)	85
GluI	GCNGC	6 cut(s)	151, 182, 825, 828, 1442, 1862
GsuI	CTGGAG	3 cut(s)	362, 771, 1532
HaeIII	GGCC	7 cut(s)	245, 267, 534, 1265, 1464, 1478, 2039
HapII	CCGG	8 cut(s)	246, 482, 506, 524, 590, 725, 1461, 1967
HgaI	GACGC	2 cut(s)	74, 700
HhaI	GCGC	1 cut(s)	86
Hin1II	CATG	9 cut(s)	49, 533, 577, 842, 1147, 1551, 1613, 1769, 1849
Hin6I	GCGC	1 cut(s)	84
HinP1I	GCGC	1 cut(s)	84
HindIII	AAGCTT	2 cut(s)	1779, 1991
HinfI	GANTC	9 cut(s)	5, 236, 319, 510, 647, 1024, 1085, 1961, 2021
HpaII	CCGG	8 cut(s)	246, 482, 506, 524, 590, 725, 1461, 1967
HphI	GGTGA	5 cut(s)	41, 172, 475, 695, 815
Hpy166II	GTNNAC	5 cut(s)	139, 715, 763, 1840, 1885
Hpy188III	TCNNGA	6 cut(s)	128, 482, 839, 1234, 1249, 1610
Hpy8I	GTNNAC	5 cut(s)	139, 715, 763, 1840, 1885
HpyCH4III	ACNGT	4 cut(s)	29, 628, 1182, 1934
HpyCH4IV	ACGT	1 cut(s)	641
HpyF10VI	GCNNNNNNNGC	6 cut(s)	833, 1052, 1149, 1457, 1861, 1990
HpyF3I	CTNAG	8 cut(s)	324, 1080, 1091, 1685, 1751, 1902, 2015, 2025
HpySE526I	ACGT	1 cut(s)	641
Hsp92II	CATG	9 cut(s)	49, 533, 577, 842, 1147, 1551, 1613, 1769, 1849
HspAI	GCGC	1 cut(s)	84
Kpn2I	TCCGGA	1 cut(s)	481
KroI	GCCGGC	2 cut(s)	245, 1460
KroNI	GCCGGC	2 cut(s)	247, 1462
Ksp22I	TGATCA	2 cut(s)	553, 570
LmnI	GCTCC	4 cut(s)	41, 1003, 1377, 1513
Lsp1109I	GCAGC	5 cut(s)	162, 168, 836, 1453, 1873
LweI	GCATC	9 cut(s)	121, 258, 282, 1161, 1401, 1497, 1668, 1708, 1734
MaeI	CTAG	3 cut(s)	62, 1158, 1590
MaeII	ACGT	1 cut(s)	641
MaeIII	GTNAC	6 cut(s)	144, 660, 703, 745, 803, 2059
MflI	RGATCY	3 cut(s)	114, 484, 1251
MhlI	GDGCHC	1 cut(s)	234
MlsI	TGGCCA	3 cut(s)	534, 1478, 2039
MluCI	AATT	7 cut(s)	435, 651, 924, 1115, 1338, 1601, 1941
MluNI	TGGCCA	3 cut(s)	534, 1478, 2039
MlyI	GAGTC	4 cut(s)	328, 656, 1033, 1970
MmeI	TCCRAC	2 cut(s)	181, 1935
Mox20I	TGGCCA	3 cut(s)	534, 1478, 2039
Mph1103I	ATGCAT	1 cut(s)	1416
MroI	TCCGGA	1 cut(s)	481
MroNI	GCCGGC	2 cut(s)	245, 1460
MroXI	GAANNNNTTC	2 cut(s)	174, 846
MscI	TGGCCA	3 cut(s)	534, 1478, 2039
MseI	TTAA	6 cut(s)	23, 585, 1619, 1791, 1995, 2074
MslI	CAYNNNNRTG	2 cut(s)	284, 1203
Msp20I	TGGCCA	3 cut(s)	534, 1478, 2039
MspA1I	CMGCKG	4 cut(s)	181, 827, 1444, 1864
MspI	CCGG	8 cut(s)	246, 482, 506, 524, 590, 725, 1461, 1967
MspR9I	CCNGG	4 cut(s)	341, 507, 1453, 1967
Mva1269I	GAATGC	1 cut(s)	174
MvaI	CCWGG	2 cut(s)	341, 1453
MwoI	GCNNNNNNNGC	6 cut(s)	833, 1052, 1149, 1457, 1861, 1990
NaeI	GCCGGC	2 cut(s)	247, 1462
NciI	CCSGG	2 cut(s)	507, 1967
NcoI	CCATGG	2 cut(s)	45, 529
NdeI	CATATG	1 cut(s)	1202
NgoMIV	GCCGGC	2 cut(s)	245, 1460
NlaIII	CATG	9 cut(s)	49, 533, 577, 842, 1147, 1551, 1613, 1769, 1849
NlaIV	GGNNCC	6 cut(s)	37, 266, 480, 522, 723, 1926
NmeAIII	GCCGAG	1 cut(s)	1066
NmuCI	GTSAC	4 cut(s)	660, 745, 803, 2059
NsbI	TGCGCA	1 cut(s)	85
NsiI	ATGCAT	1 cut(s)	1416
NspI	RCATGY	3 cut(s)	1147, 1551, 1769
NspV	TTCGAA	2 cut(s)	440, 869
OliI	CACNNNNGTG	2 cut(s)	284, 1203
PaeI	GCATGC	1 cut(s)	1147
PaeR7I	CTCGAG	1 cut(s)	316
PagI	TCATGA	2 cut(s)	838, 1609
PciI	ACATGT	1 cut(s)	1547
PcsI	WCGNNNNNNNCGW	1 cut(s)	1349
PctI	GAATGC	1 cut(s)	174
PdiI	GCCGGC	2 cut(s)	247, 1462
PdmI	GAANNNNTTC	2 cut(s)	174, 846
PfeI	GAWTC	5 cut(s)	5, 236, 510, 1085, 2021
PfoI	TCCNGGA	1 cut(s)	505
PkrI	GCNGC	6 cut(s)	152, 183, 826, 829, 1443, 1863
PleI	GAGTC	4 cut(s)	327, 655, 1032, 1969
PpsI	GAGTC	4 cut(s)	327, 655, 1032, 1969
Ppu21I	YACGTR	1 cut(s)	642
PpuMI	RGGWCCY	1 cut(s)	1925
PscI	ACATGT	1 cut(s)	1547
Psp5II	RGGWCCY	1 cut(s)	1925
Psp6I	CCWGG	2 cut(s)	339, 1451
PspGI	CCWGG	2 cut(s)	339, 1451
PspN4I	GGNNCC	6 cut(s)	37, 266, 480, 522, 723, 1926
PspPI	GGNCC	4 cut(s)	265, 526, 919, 1925
PspPPI	RGGWCCY	1 cut(s)	1925
PspXI	VCTCGAGB	1 cut(s)	316
PstNI	CAGNNNCTG	1 cut(s)	916
PsuI	RGATCY	3 cut(s)	114, 484, 1251
PvuII	CAGCTG	3 cut(s)	181, 1444, 1864
RsaI	GTAC	6 cut(s)	72, 347, 367, 1184, 1694, 1760
RsaNI	GTAC	6 cut(s)	71, 346, 366, 1183, 1693, 1759
RseI	CAYNNNNRTG	2 cut(s)	284, 1203
SaqAI	TTAA	6 cut(s)	23, 585, 1619, 1791, 1995, 2074
SatI	GCNGC	6 cut(s)	151, 182, 825, 828, 1442, 1862
Sau96I	GGNCC	4 cut(s)	265, 526, 919, 1925
ScaI	AGTACT	1 cut(s)	1184
SchI	GAGTC	4 cut(s)	328, 656, 1033, 1970
ScrFI	CCNGG	4 cut(s)	341, 507, 1453, 1967
SduI	GDGCHC	1 cut(s)	234
SfaNI	GCATC	9 cut(s)	121, 258, 282, 1161, 1401, 1497, 1668, 1708, 1734
SfcI	CTRYAG	1 cut(s)	1650
Sfr274I	CTCGAG	1 cut(s)	316
SfuI	TTCGAA	2 cut(s)	440, 869
SinI	GGWCC	3 cut(s)	526, 919, 1925
SlaI	CTCGAG	1 cut(s)	316
SmiMI	CAYNNNNRTG	2 cut(s)	284, 1203
SmlI	CTYRAG	3 cut(s)	316, 737, 1776
SmoI	CTYRAG	3 cut(s)	316, 737, 1776
SphI	GCATGC	1 cut(s)	1147
Sse9I	AATT	7 cut(s)	435, 651, 924, 1115, 1338, 1601, 1941
SsiI	CCGC	7 cut(s)	118, 134, 419, 827, 1130, 1697, 1763
SspI	AATATT	1 cut(s)	547
SspMI	CTAG	3 cut(s)	62, 1158, 1590
StyD4I	CCNGG	4 cut(s)	339, 505, 1451, 1965
StyI	CCWWGG	4 cut(s)	45, 240, 268, 529
TaaI	ACNGT	4 cut(s)	29, 628, 1182, 1934
TaiI	ACGT	1 cut(s)	644
TaqI	TCGA	6 cut(s)	8, 234, 317, 440, 869, 1343
TasI	AATT	7 cut(s)	435, 651, 924, 1115, 1338, 1601, 1941
TatI	WGTACW	2 cut(s)	345, 1182
TauI	GCSGC	1 cut(s)	830
TfiI	GAWTC	5 cut(s)	5, 236, 510, 1085, 2021
Tru1I	TTAA	6 cut(s)	23, 585, 1619, 1791, 1995, 2074
Tru9I	TTAA	6 cut(s)	23, 585, 1619, 1791, 1995, 2074
TscAI	CASTG	4 cut(s)	754, 1154, 1159, 1977
TseFI	GTSAC	4 cut(s)	660, 745, 803, 2059
TseI	GCWGC	5 cut(s)	150, 181, 824, 1441, 1861
Tsp45I	GTSAC	4 cut(s)	660, 745, 803, 2059
TspGWI	ACGGA	2 cut(s)	1436, 1553
TspRI	CASTG	4 cut(s)	754, 1154, 1159, 1977
VpaK11BI	GGWCC	3 cut(s)	526, 919, 1925
XapI	RAATTY	4 cut(s)	435, 924, 1115, 1338
XceI	RCATGY	3 cut(s)	1147, 1551, 1769
XhoI	CTCGAG	1 cut(s)	316
XmiI	GTMKAC	1 cut(s)	138
XmnI	GAANNNNTTC	2 cut(s)	174, 846
XspI	CTAG	3 cut(s)	62, 1158, 1590
ZrmI	AGTACT	1 cut(s)	1184
Zsp2I	ATGCAT	1 cut(s)	1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.