Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 299, 1478 |
| Acc65I | GGTACC | 1 cut(s) | 289 |
| AccB1I | GGYRCC | 2 cut(s) | 289, 724 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1862 |
| AccII | CGCG | 2 cut(s) | 1058, 1917 |
| AccIII | TCCGGA | 1 cut(s) | 484 |
| AciI | CCGC | 3 cut(s) | 1056, 1300, 1613 |
| AclWI | GGATC | 2 cut(s) | 58, 1084 |
| AcoI | YGGCCR | 2 cut(s) | 246, 1465 |
| AcsI | RAATTY | 6 cut(s) | 438, 897, 1101, 1118, 1127, 1288 |
| AcuI | CTGAAG | 3 cut(s) | 615, 1065, 1427 |
| AcvI | CACGTG | 1 cut(s) | 266 |
| AcyI | GRCGYC | 2 cut(s) | 135, 725 |
| AdeI | CACNNNGTG | 1 cut(s) | 671 |
| AfaI | GTAC | 5 cut(s) | 283, 291, 350, 1187, 1608 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 533, 1862 |
| AgsI | TTSAA | 8 cut(s) | 83, 239, 311, 932, 1012, 1391, 1667, 1720 |
| AjnI | CCWGG | 3 cut(s) | 243, 284, 508 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1653, 1685 |
| AleI | CACNNNNGTG | 1 cut(s) | 1206 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 321, 353, 888, 920 |
| Alw21I | GWGCWC | 1 cut(s) | 369 |
| Alw26I | GTCTC | 1 cut(s) | 1668 |
| AlwI | GGATC | 2 cut(s) | 58, 1084 |
| Ama87I | CYCGRG | 2 cut(s) | 542, 1345 |
| Aor13HI | TCCGGA | 1 cut(s) | 484 |
| AoxI | GGCC | 2 cut(s) | 246, 1465 |
| ApeKI | GCWGC | 2 cut(s) | 8, 86 |
| ApoI | RAATTY | 6 cut(s) | 438, 897, 1101, 1118, 1127, 1288 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1016, 1048 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 177 |
| Asp718I | GGTACC | 1 cut(s) | 289 |
| AspLEI | GCGC | 2 cut(s) | 727, 1917 |
| AspS9I | GGNCC | 1 cut(s) | 1853 |
| AsuHPI | GGTGA | 5 cut(s) | 56, 218, 478, 698, 1120 |
| AsuII | TTCGAA | 1 cut(s) | 1802 |
| AvaI | CYCGRG | 2 cut(s) | 542, 1345 |
| AvaII | GGWCC | 1 cut(s) | 1853 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 273, 306 |
| BalI | TGGCCA | 1 cut(s) | 1467 |
| BanI | GGYRCC | 2 cut(s) | 289, 724 |
| BbrPI | CACGTG | 1 cut(s) | 266 |
| BbsI | GAAGAC | 3 cut(s) | 1274, 1541, 1824 |
| Bbv12I | GWGCWC | 1 cut(s) | 369 |
| BbvI | GCAGC | 2 cut(s) | 20, 98 |
| BccI | CCATC | 7 cut(s) | 83, 662, 1507, 1513, 1579, 1843, 1976 |
| BceAI | ACGGC | 1 cut(s) | 122 |
| BciT130I | CCWGG | 3 cut(s) | 245, 286, 510 |
| BciVI | GTATCC | 1 cut(s) | 1576 |
| BclI | TGATCA | 2 cut(s) | 556, 573 |
| BcoDI | GTCTC | 1 cut(s) | 1668 |
| BfaI | CTAG | 4 cut(s) | 321, 519, 939, 948 |
| BfmI | CTRYAG | 1 cut(s) | 1644 |
| BfoI | RGCGCY | 1 cut(s) | 728 |
| BfuI | GTATCC | 1 cut(s) | 1576 |
| BglII | AGATCT | 1 cut(s) | 1974 |
| BisI | GCNGC | 2 cut(s) | 9, 87 |
| BlsI | GCNGC | 2 cut(s) | 10, 88 |
| BmcAI | AGTACT | 1 cut(s) | 1187 |
| Bme1390I | CCNGG | 3 cut(s) | 245, 286, 510 |
| Bme18I | GGWCC | 1 cut(s) | 1853 |
| BmeT110I | CYCGRG | 2 cut(s) | 542, 1345 |
| BmgT120I | GGNCC | 1 cut(s) | 1853 |
| BmiI | GGNNCC | 5 cut(s) | 155, 291, 726, 797, 1854 |
| BmrFI | CCNGG | 3 cut(s) | 245, 286, 510 |
| BmsI | GCATC | 5 cut(s) | 261, 382, 612, 1164, 1944 |
| BpiI | GAAGAC | 3 cut(s) | 1274, 1541, 1824 |
| BpmI | CTGGAG | 2 cut(s) | 1881, 1907 |
| Bpu10I | CCTNAGC | 2 cut(s) | 149, 1946 |
| Bpu14I | TTCGAA | 1 cut(s) | 1802 |
| BpuEI | CTTGAG | 1 cut(s) | 1677 |
| BsaAI | YACGTR | 1 cut(s) | 266 |
| BsaHI | GRCGYC | 2 cut(s) | 135, 725 |
| BsaI | GGTCTC | 1 cut(s) | 1668 |
| BsaJI | CCNNGG | 4 cut(s) | 243, 285, 508, 543 |
| BsaWI | WCCGGW | 2 cut(s) | 484, 798 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 533, 1862 |
| Bse118I | RCCGGY | 1 cut(s) | 248 |
| Bse1I | ACTGG | 2 cut(s) | 340, 902 |
| Bse3DI | GCAATG | 3 cut(s) | 384, 1150, 1309 |
| BseAI | TCCGGA | 1 cut(s) | 484 |
| BseBI | CCWGG | 3 cut(s) | 245, 286, 510 |
| BseDI | CCNNGG | 4 cut(s) | 243, 285, 508, 543 |
| BseGI | GGATG | 6 cut(s) | 101, 666, 859, 1491, 1734, 1987 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 533, 1862 |
| BseMI | GCAATG | 3 cut(s) | 384, 1150, 1309 |
| BseMII | CTCAG | 4 cut(s) | 163, 980, 1718, 1960 |
| BseNI | ACTGG | 2 cut(s) | 340, 902 |
| BseRI | GAGGAG | 3 cut(s) | 836, 1128, 1259 |
| BseXI | GCAGC | 2 cut(s) | 20, 98 |
| BsgI | GTGCAG | 1 cut(s) | 944 |
| Bsh1236I | CGCG | 2 cut(s) | 1058, 1917 |
| BshFI | GGCC | 2 cut(s) | 248, 1467 |
| BshNI | GGYRCC | 2 cut(s) | 289, 724 |
| BsiHKAI | GWGCWC | 1 cut(s) | 369 |
| BsiHKCI | CYCGRG | 2 cut(s) | 542, 1345 |
| BsiSI | CCGG | 3 cut(s) | 249, 485, 799 |
| BslFI | GGGAC | 1 cut(s) | 1866 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 533, 1862 |
| BsmAI | GTCTC | 1 cut(s) | 1668 |
| BsmFI | GGGAC | 1 cut(s) | 1866 |
| BsmI | GAATGC | 1 cut(s) | 177 |
| BsnI | GGCC | 2 cut(s) | 248, 1467 |
| Bso31I | GGTCTC | 1 cut(s) | 1668 |
| BsoBI | CYCGRG | 2 cut(s) | 542, 1345 |
| Bsp119I | TTCGAA | 1 cut(s) | 1802 |
| Bsp1286I | GDGCHC | 1 cut(s) | 369 |
| Bsp13I | TCCGGA | 1 cut(s) | 484 |
| Bsp1407I | TGTACA | 1 cut(s) | 1606 |
| BspACI | CCGC | 3 cut(s) | 1056, 1300, 1613 |
| BspANI | GGCC | 2 cut(s) | 248, 1467 |
| BspCNI | CTCAG | 4 cut(s) | 162, 981, 1717, 1959 |
| BspEI | TCCGGA | 1 cut(s) | 484 |
| BspFNI | CGCG | 2 cut(s) | 1058, 1917 |
| BspLI | GGNNCC | 5 cut(s) | 155, 291, 726, 797, 1854 |
| BspPI | GGATC | 2 cut(s) | 58, 1084 |
| BspQI | GCTCTTC | 1 cut(s) | 1453 |
| BspT104I | TTCGAA | 1 cut(s) | 1802 |
| BspT107I | GGYRCC | 2 cut(s) | 289, 724 |
| BspTNI | GGTCTC | 1 cut(s) | 1668 |
| BsrDI | GCAATG | 3 cut(s) | 384, 1150, 1309 |
| BsrFI | RCCGGY | 1 cut(s) | 248 |
| BsrGI | TGTACA | 1 cut(s) | 1606 |
| BsrI | ACTGG | 2 cut(s) | 340, 902 |
| BssAI | RCCGGY | 1 cut(s) | 248 |
| BssECI | CCNNGG | 4 cut(s) | 243, 285, 508, 543 |
| BssNI | GRCGYC | 2 cut(s) | 135, 725 |
| Bst2UI | CCWGG | 3 cut(s) | 245, 286, 510 |
| Bst4CI | ACNGT | 1 cut(s) | 631 |
| Bst6I | CTCTTC | 2 cut(s) | 591, 1453 |
| BstACI | GRCGYC | 2 cut(s) | 135, 725 |
| BstAUI | TGTACA | 1 cut(s) | 1606 |
| BstBAI | YACGTR | 1 cut(s) | 266 |
| BstBI | TTCGAA | 1 cut(s) | 1802 |
| BstC8I | GCNNGC | 5 cut(s) | 31, 250, 260, 1465, 1580 |
| BstDEI | CTNAG | 8 cut(s) | 149, 989, 1094, 1237, 1704, 1754, 1946, 1971 |
| BstEII | GGTNACC | 1 cut(s) | 206 |
| BstF5I | GGATG | 6 cut(s) | 101, 666, 859, 1491, 1734, 1987 |
| BstFNI | CGCG | 2 cut(s) | 1058, 1917 |
| BstH2I | RGCGCY | 1 cut(s) | 728 |
| BstHHI | GCGC | 2 cut(s) | 727, 1917 |
| BstMAI | GTCTC | 1 cut(s) | 1668 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 228, 1055, 1921 |
| BstNI | CCWGG | 3 cut(s) | 245, 286, 510 |
| BstNSI | RCATGY | 4 cut(s) | 33, 74, 1150, 1613 |
| BstPI | GGTNACC | 1 cut(s) | 206 |
| BstSCI | CCNGG | 3 cut(s) | 243, 284, 508 |
| BstSFI | CTRYAG | 1 cut(s) | 1644 |
| BstUI | CGCG | 2 cut(s) | 1058, 1917 |
| BstV1I | GCAGC | 2 cut(s) | 20, 98 |
| BstV2I | GAAGAC | 3 cut(s) | 1274, 1541, 1824 |
| BstX2I | RGATCY | 1 cut(s) | 1974 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 218 |
| BstYI | RGATCY | 1 cut(s) | 1974 |
| BsuI | GTATCC | 1 cut(s) | 1576 |
| BsuRI | GGCC | 2 cut(s) | 248, 1467 |
| BtsCI | GGATG | 6 cut(s) | 101, 666, 859, 1491, 1734, 1987 |
| BtsI | GCAGTG | 1 cut(s) | 1155 |
| BtsIMutI | CAGTG | 2 cut(s) | 369, 1155 |
| Cac8I | GCNNGC | 5 cut(s) | 31, 250, 260, 1465, 1580 |
| CfoI | GCGC | 2 cut(s) | 727, 1917 |
| Cfr10I | RCCGGY | 1 cut(s) | 248 |
| Cfr13I | GGNCC | 1 cut(s) | 1853 |
| CseI | GACGC | 2 cut(s) | 143, 220 |
| Csp6I | GTAC | 5 cut(s) | 282, 290, 349, 1186, 1607 |
| CviQI | GTAC | 5 cut(s) | 282, 290, 349, 1186, 1607 |
| DdeI | CTNAG | 8 cut(s) | 149, 989, 1094, 1237, 1704, 1754, 1946, 1971 |
| DinI | GGCGCC | 1 cut(s) | 726 |
| DraI | TTTAAA | 1 cut(s) | 853 |
| DraIII | CACNNNGTG | 1 cut(s) | 671 |
| EaeI | YGGCCR | 2 cut(s) | 246, 1465 |
| Eam1104I | CTCTTC | 2 cut(s) | 591, 1453 |
| EarI | CTCTTC | 2 cut(s) | 591, 1453 |
| Eco31I | GGTCTC | 1 cut(s) | 1668 |
| Eco32I | GATATC | 1 cut(s) | 550 |
| Eco47I | GGWCC | 1 cut(s) | 1853 |
| Eco57I | CTGAAG | 3 cut(s) | 615, 1065, 1427 |
| Eco72I | CACGTG | 1 cut(s) | 266 |
| Eco88I | CYCGRG | 2 cut(s) | 542, 1345 |
| Eco91I | GGTNACC | 1 cut(s) | 206 |
| EcoO65I | GGTNACC | 1 cut(s) | 206 |
| EcoRI | GAATTC | 2 cut(s) | 897, 1127 |
| EcoRII | CCWGG | 3 cut(s) | 243, 284, 508 |
| EcoRV | GATATC | 1 cut(s) | 550 |
| EcoT22I | ATGCAT | 3 cut(s) | 35, 1152, 1419 |
| EgeI | GGCGCC | 1 cut(s) | 726 |
| EheI | GGCGCC | 1 cut(s) | 726 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1077, 1109 |
| FaqI | GGGAC | 1 cut(s) | 1866 |
| FauI | CCCGC | 1 cut(s) | 1307 |
| FbaI | TGATCA | 2 cut(s) | 556, 573 |
| Fnu4HI | GCNGC | 2 cut(s) | 9, 87 |
| FokI | GGATG | 6 cut(s) | 88, 673, 846, 1498, 1741, 1994 |
| Fsp4HI | GCNGC | 2 cut(s) | 9, 87 |
| FspBI | CTAG | 4 cut(s) | 321, 519, 939, 948 |
| GlaI | GCGC | 2 cut(s) | 726, 1916 |
| GluI | GCNGC | 2 cut(s) | 9, 87 |
| GsuI | CTGGAG | 2 cut(s) | 1881, 1907 |
| HaeII | RGCGCY | 1 cut(s) | 728 |
| HaeIII | GGCC | 2 cut(s) | 248, 1467 |
| HapII | CCGG | 3 cut(s) | 249, 485, 799 |
| HgaI | GACGC | 2 cut(s) | 143, 220 |
| HhaI | GCGC | 2 cut(s) | 727, 1917 |
| Hin1I | GRCGYC | 2 cut(s) | 135, 725 |
| Hin6I | GCGC | 2 cut(s) | 725, 1915 |
| HinP1I | GCGC | 2 cut(s) | 725, 1915 |
| HincII | GTYRAC | 2 cut(s) | 142, 1592 |
| HindII | GTYRAC | 2 cut(s) | 142, 1592 |
| HindIII | AAGCTT | 1 cut(s) | 933 |
| HpaII | CCGG | 3 cut(s) | 249, 485, 799 |
| HphI | GGTGA | 5 cut(s) | 56, 218, 478, 698, 1120 |
| Hpy166II | GTNNAC | 5 cut(s) | 142, 269, 1509, 1592, 1813 |
| Hpy188I | TCNGA | 4 cut(s) | 40, 1076, 1287, 1570 |
| Hpy188III | TCNNGA | 8 cut(s) | 151, 326, 485, 658, 970, 1252, 1345, 1556 |
| Hpy8I | GTNNAC | 5 cut(s) | 142, 269, 1509, 1592, 1813 |
| HpyAV | CCTTC | 8 cut(s) | 35, 41, 167, 725, 739, 1330, 1688, 1752 |
| HpyCH4III | ACNGT | 1 cut(s) | 631 |
| HpyCH4IV | ACGT | 1 cut(s) | 265 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 228, 1055, 1921 |
| HpyF3I | CTNAG | 8 cut(s) | 149, 989, 1094, 1237, 1704, 1754, 1946, 1971 |
| HpySE526I | ACGT | 1 cut(s) | 265 |
| Hsp92I | GRCGYC | 2 cut(s) | 135, 725 |
| HspAI | GCGC | 2 cut(s) | 725, 1915 |
| KasI | GGCGCC | 1 cut(s) | 724 |
| Kpn2I | TCCGGA | 1 cut(s) | 484 |
| KpnI | GGTACC | 1 cut(s) | 293 |
| KroI | GCCGGC | 1 cut(s) | 248 |
| KroNI | GCCGGC | 1 cut(s) | 250 |
| Ksp22I | TGATCA | 2 cut(s) | 556, 573 |
| LguI | GCTCTTC | 1 cut(s) | 1453 |
| LmnI | GCTCC | 4 cut(s) | 5, 153, 189, 1575 |
| Lsp1109I | GCAGC | 2 cut(s) | 20, 98 |
| LweI | GCATC | 5 cut(s) | 261, 382, 612, 1164, 1944 |
| MaeI | CTAG | 4 cut(s) | 321, 519, 939, 948 |
| MaeII | ACGT | 1 cut(s) | 265 |
| MaeIII | GTNAC | 6 cut(s) | 206, 663, 706, 748, 1217, 1229 |
| MboII | GAAGA | 8 cut(s) | 608, 985, 1223, 1274, 1403, 1470, 1546, 1829 |
| MflI | RGATCY | 1 cut(s) | 1974 |
| MhlI | GDGCHC | 1 cut(s) | 369 |
| MlsI | TGGCCA | 1 cut(s) | 1467 |
| MluCI | AATT | 7 cut(s) | 438, 897, 1101, 1118, 1127, 1288, 1858 |
| MluNI | TGGCCA | 1 cut(s) | 1467 |
| Mly113I | GGCGCC | 1 cut(s) | 725 |
| MlyI | GAGTC | 3 cut(s) | 688, 1036, 1625 |
| MmeI | TCCRAC | 3 cut(s) | 324, 874, 1651 |
| Mox20I | TGGCCA | 1 cut(s) | 1467 |
| Mph1103I | ATGCAT | 3 cut(s) | 35, 1152, 1419 |
| MroI | TCCGGA | 1 cut(s) | 484 |
| MroNI | GCCGGC | 1 cut(s) | 248 |
| MroXI | GAANNNNTTC | 1 cut(s) | 177 |
| MscI | TGGCCA | 1 cut(s) | 1467 |
| MseI | TTAA | 4 cut(s) | 852, 1167, 1412, 1929 |
| MslI | CAYNNNNRTG | 5 cut(s) | 164, 372, 575, 659, 1206 |
| Msp20I | TGGCCA | 1 cut(s) | 1467 |
| MspI | CCGG | 3 cut(s) | 249, 485, 799 |
| MspR9I | CCNGG | 3 cut(s) | 245, 286, 510 |
| Mva1269I | GAATGC | 1 cut(s) | 177 |
| MvaI | CCWGG | 3 cut(s) | 245, 286, 510 |
| MvnI | CGCG | 2 cut(s) | 1058, 1917 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 228, 1055, 1921 |
| NaeI | GCCGGC | 1 cut(s) | 250 |
| NarI | GGCGCC | 1 cut(s) | 725 |
| NgoMIV | GCCGGC | 1 cut(s) | 248 |
| NlaIV | GGNNCC | 5 cut(s) | 155, 291, 726, 797, 1854 |
| NmeAIII | GCCGAG | 1 cut(s) | 1141 |
| NmuCI | GTSAC | 4 cut(s) | 206, 663, 706, 1217 |
| NsiI | ATGCAT | 3 cut(s) | 35, 1152, 1419 |
| NspI | RCATGY | 4 cut(s) | 33, 74, 1150, 1613 |
| NspV | TTCGAA | 1 cut(s) | 1802 |
| OliI | CACNNNNGTG | 1 cut(s) | 1206 |
| PaeI | GCATGC | 1 cut(s) | 33 |
| PaeR7I | CTCGAG | 1 cut(s) | 1345 |
| PciSI | GCTCTTC | 1 cut(s) | 1453 |
| PctI | GAATGC | 1 cut(s) | 177 |
| PdiI | GCCGGC | 1 cut(s) | 250 |
| PdmI | GAANNNNTTC | 1 cut(s) | 177 |
| PfeI | GAWTC | 8 cut(s) | 239, 513, 650, 928, 1088, 1341, 1892, 1952 |
| PflFI | GACNNNGTC | 1 cut(s) | 965 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1862 |
| PkrI | GCNGC | 2 cut(s) | 10, 88 |
| PleI | GAGTC | 3 cut(s) | 688, 1035, 1625 |
| PluTI | GGCGCC | 1 cut(s) | 728 |
| PmaCI | CACGTG | 1 cut(s) | 266 |
| PmlI | CACGTG | 1 cut(s) | 266 |
| PpsI | GAGTC | 3 cut(s) | 688, 1035, 1625 |
| Ppu21I | YACGTR | 1 cut(s) | 266 |
| PsiI | TTATAA | 2 cut(s) | 299, 1478 |
| Psp6I | CCWGG | 3 cut(s) | 243, 284, 508 |
| PspCI | CACGTG | 1 cut(s) | 266 |
| PspEI | GGTNACC | 1 cut(s) | 206 |
| PspGI | CCWGG | 3 cut(s) | 243, 284, 508 |
| PspN4I | GGNNCC | 5 cut(s) | 155, 291, 726, 797, 1854 |
| PspPI | GGNCC | 1 cut(s) | 1853 |
| PsuI | RGATCY | 1 cut(s) | 1974 |
| PsyI | GACNNNGTC | 1 cut(s) | 965 |
| RsaI | GTAC | 5 cut(s) | 283, 291, 350, 1187, 1608 |
| RsaNI | GTAC | 5 cut(s) | 282, 290, 349, 1186, 1607 |
| RseI | CAYNNNNRTG | 5 cut(s) | 164, 372, 575, 659, 1206 |
| SapI | GCTCTTC | 1 cut(s) | 1453 |
| SaqAI | TTAA | 4 cut(s) | 852, 1167, 1412, 1929 |
| SatI | GCNGC | 2 cut(s) | 9, 87 |
| Sau96I | GGNCC | 1 cut(s) | 1853 |
| ScaI | AGTACT | 1 cut(s) | 1187 |
| SchI | GAGTC | 3 cut(s) | 688, 1036, 1625 |
| ScrFI | CCNGG | 3 cut(s) | 245, 286, 510 |
| SduI | GDGCHC | 1 cut(s) | 369 |
| SfaNI | GCATC | 5 cut(s) | 261, 382, 612, 1164, 1944 |
| SfcI | CTRYAG | 1 cut(s) | 1644 |
| SfoI | GGCGCC | 1 cut(s) | 726 |
| Sfr274I | CTCGAG | 1 cut(s) | 1345 |
| SfuI | TTCGAA | 1 cut(s) | 1802 |
| SinI | GGWCC | 1 cut(s) | 1853 |
| SlaI | CTCGAG | 1 cut(s) | 1345 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 164, 372, 575, 659, 1206 |
| SmlI | CTYRAG | 2 cut(s) | 1345, 1692 |
| SmoI | CTYRAG | 2 cut(s) | 1345, 1692 |
| SphI | GCATGC | 1 cut(s) | 33 |
| Sse9I | AATT | 7 cut(s) | 438, 897, 1101, 1118, 1127, 1288, 1858 |
| SsiI | CCGC | 3 cut(s) | 1056, 1300, 1613 |
| SspDI | GGCGCC | 1 cut(s) | 724 |
| SspMI | CTAG | 4 cut(s) | 321, 519, 939, 948 |
| StyD4I | CCNGG | 3 cut(s) | 243, 284, 508 |
| TaaI | ACNGT | 1 cut(s) | 631 |
| TaiI | ACGT | 1 cut(s) | 268 |
| TaqI | TCGA | 2 cut(s) | 1346, 1802 |
| TasI | AATT | 7 cut(s) | 438, 897, 1101, 1118, 1127, 1288, 1858 |
| TatI | WGTACW | 3 cut(s) | 348, 1185, 1606 |
| TfiI | GAWTC | 8 cut(s) | 239, 513, 650, 928, 1088, 1341, 1892, 1952 |
| Tru1I | TTAA | 4 cut(s) | 852, 1167, 1412, 1929 |
| Tru9I | TTAA | 4 cut(s) | 852, 1167, 1412, 1929 |
| TscAI | CASTG | 2 cut(s) | 376, 1162 |
| TseFI | GTSAC | 4 cut(s) | 206, 663, 706, 1217 |
| TseI | GCWGC | 2 cut(s) | 8, 86 |
| Tsp45I | GTSAC | 4 cut(s) | 206, 663, 706, 1217 |
| TspDTI | ATGAA | 4 cut(s) | 521, 825, 1140, 1502 |
| TspGWI | ACGGA | 1 cut(s) | 110 |
| TspRI | CASTG | 2 cut(s) | 376, 1162 |
| Tth111I | GACNNNGTC | 1 cut(s) | 965 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1862 |
| VpaK11BI | GGWCC | 1 cut(s) | 1853 |
| XapI | RAATTY | 6 cut(s) | 438, 897, 1101, 1118, 1127, 1288 |
| XceI | RCATGY | 4 cut(s) | 33, 74, 1150, 1613 |
| XhoI | CTCGAG | 1 cut(s) | 1345 |
| XmnI | GAANNNNTTC | 1 cut(s) | 177 |
| XspI | CTAG | 4 cut(s) | 321, 519, 939, 948 |
| ZrmI | AGTACT | 1 cut(s) | 1187 |
| Zsp2I | ATGCAT | 3 cut(s) | 35, 1152, 1419 |