Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 488, 1667 |
| Acc65I | GGTACC | 1 cut(s) | 478 |
| AccB1I | GGYRCC | 3 cut(s) | 134, 478, 913 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2017, 2057 |
| AccII | CGCG | 2 cut(s) | 1247, 2112 |
| AccIII | TCCGGA | 1 cut(s) | 673 |
| AciI | CCGC | 2 cut(s) | 1245, 1805 |
| AclWI | GGATC | 2 cut(s) | 247, 1931 |
| AcoI | YGGCCR | 2 cut(s) | 435, 1654 |
| AcsI | RAATTY | 6 cut(s) | 627, 1086, 1290, 1307, 1316, 1477 |
| AcuI | CTGAAG | 3 cut(s) | 804, 1254, 1616 |
| AcvI | CACGTG | 1 cut(s) | 455 |
| AcyI | GRCGYC | 3 cut(s) | 135, 324, 914 |
| AdeI | CACNNNGTG | 1 cut(s) | 860 |
| AfaI | GTAC | 5 cut(s) | 480, 539, 1376, 1800, 1890 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 131, 722, 892, 2017, 2057 |
| AjnI | CCWGG | 4 cut(s) | 432, 473, 697, 885 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1845, 1877 |
| AleI | CACNNNNGTG | 1 cut(s) | 1395 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 510, 542, 1077, 1109 |
| Alw21I | GWGCWC | 1 cut(s) | 558 |
| Alw26I | GTCTC | 1 cut(s) | 1860 |
| AlwI | GGATC | 2 cut(s) | 247, 1931 |
| Ama87I | CYCGRG | 2 cut(s) | 731, 1534 |
| Aor13HI | TCCGGA | 1 cut(s) | 673 |
| AoxI | GGCC | 2 cut(s) | 435, 1654 |
| ApeKI | GCWGC | 3 cut(s) | 8, 86, 275 |
| ApoI | RAATTY | 6 cut(s) | 627, 1086, 1290, 1307, 1316, 1477 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1205, 1237 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 366 |
| Asp718I | GGTACC | 1 cut(s) | 478 |
| AspLEI | GCGC | 3 cut(s) | 137, 916, 2112 |
| AspS9I | GGNCC | 2 cut(s) | 882, 2048 |
| AsuHPI | GGTGA | 5 cut(s) | 56, 245, 407, 667, 887 |
| AvaI | CYCGRG | 2 cut(s) | 731, 1534 |
| AvaII | GGWCC | 2 cut(s) | 882, 2048 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 462, 495 |
| BalI | TGGCCA | 1 cut(s) | 1656 |
| BanI | GGYRCC | 3 cut(s) | 134, 478, 913 |
| BbrPI | CACGTG | 1 cut(s) | 455 |
| BbsI | GAAGAC | 1 cut(s) | 1463 |
| Bbv12I | GWGCWC | 1 cut(s) | 558 |
| BbvI | GCAGC | 3 cut(s) | 20, 98, 287 |
| BccI | CCATC | 8 cut(s) | 83, 184, 851, 1696, 1702, 1771, 2038, 2171 |
| BceAI | ACGGC | 1 cut(s) | 248 |
| BciT130I | CCWGG | 4 cut(s) | 434, 475, 699, 887 |
| BciVI | GTATCC | 1 cut(s) | 1890 |
| BclI | TGATCA | 3 cut(s) | 159, 745, 762 |
| BcoDI | GTCTC | 1 cut(s) | 1860 |
| BfaI | CTAG | 4 cut(s) | 510, 708, 1128, 1137 |
| BfmI | CTRYAG | 1 cut(s) | 1836 |
| BfoI | RGCGCY | 2 cut(s) | 138, 917 |
| BfuI | GTATCC | 1 cut(s) | 1890 |
| BisI | GCNGC | 3 cut(s) | 9, 87, 276 |
| BlsI | GCNGC | 3 cut(s) | 10, 88, 277 |
| BmcAI | AGTACT | 2 cut(s) | 1376, 1890 |
| Bme1390I | CCNGG | 4 cut(s) | 434, 475, 699, 887 |
| Bme18I | GGWCC | 2 cut(s) | 882, 2048 |
| BmeT110I | CYCGRG | 2 cut(s) | 731, 1534 |
| BmgT120I | GGNCC | 2 cut(s) | 882, 2048 |
| BmiI | GGNNCC | 8 cut(s) | 136, 155, 344, 480, 884, 915, 986, 2049 |
| BmrFI | CCNGG | 4 cut(s) | 434, 475, 699, 887 |
| BmsI | GCATC | 5 cut(s) | 206, 450, 801, 1353, 2139 |
| BpiI | GAAGAC | 1 cut(s) | 1463 |
| BpmI | CTGGAG | 1 cut(s) | 2076 |
| Bpu10I | CCTNAGC | 2 cut(s) | 149, 338 |
| BpuEI | CTTGAG | 2 cut(s) | 1869, 2126 |
| BsaAI | YACGTR | 1 cut(s) | 455 |
| BsaHI | GRCGYC | 3 cut(s) | 135, 324, 914 |
| BsaI | GGTCTC | 1 cut(s) | 1860 |
| BsaJI | CCNNGG | 6 cut(s) | 130, 432, 474, 697, 732, 885 |
| BsaWI | WCCGGW | 3 cut(s) | 528, 673, 987 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 131, 722, 892, 2017, 2057 |
| Bse118I | RCCGGY | 1 cut(s) | 437 |
| Bse1I | ACTGG | 2 cut(s) | 1091, 2178 |
| Bse3DI | GCAATG | 1 cut(s) | 1339 |
| BseAI | TCCGGA | 1 cut(s) | 673 |
| BseBI | CCWGG | 4 cut(s) | 434, 475, 699, 887 |
| BseDI | CCNNGG | 6 cut(s) | 130, 432, 474, 697, 732, 885 |
| BseGI | GGATG | 6 cut(s) | 101, 290, 855, 1048, 1680, 2182 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 131, 722, 892, 2017, 2057 |
| BseMI | GCAATG | 1 cut(s) | 1339 |
| BseMII | CTCAG | 6 cut(s) | 163, 352, 513, 1169, 1392, 1913 |
| BseNI | ACTGG | 2 cut(s) | 1091, 2178 |
| BseRI | GAGGAG | 3 cut(s) | 1025, 1317, 1448 |
| BseXI | GCAGC | 3 cut(s) | 20, 98, 287 |
| BsgI | GTGCAG | 2 cut(s) | 454, 1133 |
| Bsh1236I | CGCG | 2 cut(s) | 1247, 2112 |
| BshFI | GGCC | 2 cut(s) | 437, 1656 |
| BshNI | GGYRCC | 3 cut(s) | 134, 478, 913 |
| BsiHKAI | GWGCWC | 1 cut(s) | 558 |
| BsiHKCI | CYCGRG | 2 cut(s) | 731, 1534 |
| BsiSI | CCGG | 4 cut(s) | 438, 529, 674, 988 |
| BslFI | GGGAC | 1 cut(s) | 2061 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 131, 722, 892, 2017, 2057 |
| BsmAI | GTCTC | 1 cut(s) | 1860 |
| BsmFI | GGGAC | 1 cut(s) | 2061 |
| BsmI | GAATGC | 1 cut(s) | 366 |
| BsnI | GGCC | 2 cut(s) | 437, 1656 |
| Bso31I | GGTCTC | 1 cut(s) | 1860 |
| BsoBI | CYCGRG | 2 cut(s) | 731, 1534 |
| Bsp1286I | GDGCHC | 1 cut(s) | 558 |
| Bsp13I | TCCGGA | 1 cut(s) | 673 |
| Bsp1407I | TGTACA | 1 cut(s) | 1798 |
| BspACI | CCGC | 2 cut(s) | 1245, 1805 |
| BspANI | GGCC | 2 cut(s) | 437, 1656 |
| BspCNI | CTCAG | 6 cut(s) | 162, 351, 514, 1170, 1391, 1912 |
| BspEI | TCCGGA | 1 cut(s) | 673 |
| BspFNI | CGCG | 2 cut(s) | 1247, 2112 |
| BspLI | GGNNCC | 8 cut(s) | 136, 155, 344, 480, 884, 915, 986, 2049 |
| BspPI | GGATC | 2 cut(s) | 247, 1931 |
| BspQI | GCTCTTC | 1 cut(s) | 1642 |
| BspT107I | GGYRCC | 3 cut(s) | 134, 478, 913 |
| BspTNI | GGTCTC | 1 cut(s) | 1860 |
| BsrDI | GCAATG | 1 cut(s) | 1339 |
| BsrFI | RCCGGY | 1 cut(s) | 437 |
| BsrGI | TGTACA | 1 cut(s) | 1798 |
| BsrI | ACTGG | 2 cut(s) | 1091, 2178 |
| BssAI | RCCGGY | 1 cut(s) | 437 |
| BssECI | CCNNGG | 6 cut(s) | 130, 432, 474, 697, 732, 885 |
| BssNI | GRCGYC | 3 cut(s) | 135, 324, 914 |
| BssT1I | CCWWGG | 1 cut(s) | 130 |
| Bst2UI | CCWGG | 4 cut(s) | 434, 475, 699, 887 |
| Bst4CI | ACNGT | 1 cut(s) | 820 |
| Bst6I | CTCTTC | 2 cut(s) | 780, 1642 |
| BstACI | GRCGYC | 3 cut(s) | 135, 324, 914 |
| BstAUI | TGTACA | 1 cut(s) | 1798 |
| BstBAI | YACGTR | 1 cut(s) | 455 |
| BstC8I | GCNNGC | 6 cut(s) | 31, 220, 439, 449, 1654, 1772 |
| BstEII | GGTNACC | 1 cut(s) | 395 |
| BstF5I | GGATG | 6 cut(s) | 101, 290, 855, 1048, 1680, 2182 |
| BstFNI | CGCG | 2 cut(s) | 1247, 2112 |
| BstH2I | RGCGCY | 2 cut(s) | 138, 917 |
| BstHHI | GCGC | 3 cut(s) | 137, 916, 2112 |
| BstMAI | GTCTC | 1 cut(s) | 1860 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 417, 1244, 2116 |
| BstNI | CCWGG | 4 cut(s) | 434, 475, 699, 887 |
| BstNSI | RCATGY | 6 cut(s) | 33, 74, 222, 263, 1339, 1805 |
| BstPI | GGTNACC | 1 cut(s) | 395 |
| BstSCI | CCNGG | 4 cut(s) | 432, 473, 697, 885 |
| BstSFI | CTRYAG | 1 cut(s) | 1836 |
| BstUI | CGCG | 2 cut(s) | 1247, 2112 |
| BstV1I | GCAGC | 3 cut(s) | 20, 98, 287 |
| BstV2I | GAAGAC | 1 cut(s) | 1463 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 407 |
| BsuI | GTATCC | 1 cut(s) | 1890 |
| BsuRI | GGCC | 2 cut(s) | 437, 1656 |
| BtsCI | GGATG | 6 cut(s) | 101, 290, 855, 1048, 1680, 2182 |
| BtsI | GCAGTG | 2 cut(s) | 1344, 1926 |
| BtsIMutI | CAGTG | 3 cut(s) | 558, 1344, 1926 |
| Cac8I | GCNNGC | 6 cut(s) | 31, 220, 439, 449, 1654, 1772 |
| CfoI | GCGC | 3 cut(s) | 137, 916, 2112 |
| Cfr10I | RCCGGY | 1 cut(s) | 437 |
| Cfr13I | GGNCC | 2 cut(s) | 882, 2048 |
| CseI | GACGC | 3 cut(s) | 332, 409, 1799 |
| Csp6I | GTAC | 5 cut(s) | 479, 538, 1375, 1799, 1889 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 367, 402 |
| CviQI | GTAC | 5 cut(s) | 479, 538, 1375, 1799, 1889 |
| DinI | GGCGCC | 2 cut(s) | 136, 915 |
| DraIII | CACNNNGTG | 1 cut(s) | 860 |
| EaeI | YGGCCR | 2 cut(s) | 435, 1654 |
| Eam1104I | CTCTTC | 2 cut(s) | 780, 1642 |
| EarI | CTCTTC | 2 cut(s) | 780, 1642 |
| Eco130I | CCWWGG | 1 cut(s) | 130 |
| Eco31I | GGTCTC | 1 cut(s) | 1860 |
| Eco32I | GATATC | 1 cut(s) | 739 |
| Eco47I | GGWCC | 2 cut(s) | 882, 2048 |
| Eco57I | CTGAAG | 3 cut(s) | 804, 1254, 1616 |
| Eco72I | CACGTG | 1 cut(s) | 455 |
| Eco88I | CYCGRG | 2 cut(s) | 731, 1534 |
| Eco91I | GGTNACC | 1 cut(s) | 395 |
| EcoO109I | RGGNCCY | 1 cut(s) | 882 |
| EcoO65I | GGTNACC | 1 cut(s) | 395 |
| EcoRI | GAATTC | 1 cut(s) | 1086 |
| EcoRII | CCWGG | 4 cut(s) | 432, 473, 697, 885 |
| EcoRV | GATATC | 1 cut(s) | 739 |
| EcoT14I | CCWWGG | 1 cut(s) | 130 |
| EcoT22I | ATGCAT | 4 cut(s) | 35, 224, 1341, 1608 |
| EgeI | GGCGCC | 2 cut(s) | 136, 915 |
| EheI | GGCGCC | 2 cut(s) | 136, 915 |
| ErhI | CCWWGG | 1 cut(s) | 130 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1266, 1298 |
| FaqI | GGGAC | 1 cut(s) | 2061 |
| FbaI | TGATCA | 3 cut(s) | 159, 745, 762 |
| Fnu4HI | GCNGC | 3 cut(s) | 9, 87, 276 |
| FokI | GGATG | 6 cut(s) | 88, 277, 862, 1035, 1687, 2189 |
| Fsp4HI | GCNGC | 3 cut(s) | 9, 87, 276 |
| FspBI | CTAG | 4 cut(s) | 510, 708, 1128, 1137 |
| GlaI | GCGC | 3 cut(s) | 136, 915, 2111 |
| GluI | GCNGC | 3 cut(s) | 9, 87, 276 |
| GsuI | CTGGAG | 1 cut(s) | 2076 |
| HaeII | RGCGCY | 2 cut(s) | 138, 917 |
| HaeIII | GGCC | 2 cut(s) | 437, 1656 |
| HapII | CCGG | 4 cut(s) | 438, 529, 674, 988 |
| HgaI | GACGC | 3 cut(s) | 332, 409, 1799 |
| HhaI | GCGC | 3 cut(s) | 137, 916, 2112 |
| Hin1I | GRCGYC | 3 cut(s) | 135, 324, 914 |
| Hin6I | GCGC | 3 cut(s) | 135, 914, 2110 |
| HinP1I | GCGC | 3 cut(s) | 135, 914, 2110 |
| HincII | GTYRAC | 1 cut(s) | 1784 |
| HindII | GTYRAC | 1 cut(s) | 1784 |
| HindIII | AAGCTT | 1 cut(s) | 1122 |
| HpaII | CCGG | 4 cut(s) | 438, 529, 674, 988 |
| HphI | GGTGA | 5 cut(s) | 56, 245, 407, 667, 887 |
| Hpy166II | GTNNAC | 4 cut(s) | 458, 1698, 1784, 2008 |
| Hpy188I | TCNGA | 9 cut(s) | 40, 177, 229, 1195, 1265, 1296, 1381, 1476, 1762 |
| Hpy8I | GTNNAC | 4 cut(s) | 458, 1698, 1784, 2008 |
| HpyCH4III | ACNGT | 1 cut(s) | 820 |
| HpyCH4IV | ACGT | 1 cut(s) | 454 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 417, 1244, 2116 |
| HpySE526I | ACGT | 1 cut(s) | 454 |
| Hsp92I | GRCGYC | 3 cut(s) | 135, 324, 914 |
| HspAI | GCGC | 3 cut(s) | 135, 914, 2110 |
| KasI | GGCGCC | 2 cut(s) | 134, 913 |
| Kpn2I | TCCGGA | 1 cut(s) | 673 |
| KpnI | GGTACC | 1 cut(s) | 482 |
| KroI | GCCGGC | 1 cut(s) | 437 |
| KroNI | GCCGGC | 1 cut(s) | 439 |
| Ksp22I | TGATCA | 3 cut(s) | 159, 745, 762 |
| LguI | GCTCTTC | 1 cut(s) | 1642 |
| LmnI | GCTCC | 6 cut(s) | 5, 153, 194, 342, 378, 1767 |
| Lsp1109I | GCAGC | 3 cut(s) | 20, 98, 287 |
| LweI | GCATC | 5 cut(s) | 206, 450, 801, 1353, 2139 |
| MaeI | CTAG | 4 cut(s) | 510, 708, 1128, 1137 |
| MaeII | ACGT | 1 cut(s) | 454 |
| MaeIII | GTNAC | 6 cut(s) | 395, 852, 895, 937, 1406, 1418 |
| MboII | GAAGA | 7 cut(s) | 199, 797, 1174, 1412, 1463, 1592, 1659 |
| MfeI | CAATTG | 1 cut(s) | 282 |
| MhlI | GDGCHC | 1 cut(s) | 558 |
| MlsI | TGGCCA | 1 cut(s) | 1656 |
| MluCI | AATT | 9 cut(s) | 282, 573, 627, 1086, 1290, 1307, 1316, 1477, 2053 |
| MluNI | TGGCCA | 1 cut(s) | 1656 |
| Mly113I | GGCGCC | 2 cut(s) | 135, 914 |
| MlyI | GAGTC | 1 cut(s) | 1225 |
| MmeI | TCCRAC | 4 cut(s) | 513, 1063, 1528, 1843 |
| Mox20I | TGGCCA | 1 cut(s) | 1656 |
| Mph1103I | ATGCAT | 4 cut(s) | 35, 224, 1341, 1608 |
| MroI | TCCGGA | 1 cut(s) | 673 |
| MroNI | GCCGGC | 1 cut(s) | 437 |
| MroXI | GAANNNNTTC | 1 cut(s) | 366 |
| MscI | TGGCCA | 1 cut(s) | 1656 |
| MseI | TTAA | 2 cut(s) | 1601, 2124 |
| MslI | CAYNNNNRTG | 5 cut(s) | 353, 561, 764, 848, 1395 |
| Msp20I | TGGCCA | 1 cut(s) | 1656 |
| MspI | CCGG | 4 cut(s) | 438, 529, 674, 988 |
| MspR9I | CCNGG | 4 cut(s) | 434, 475, 699, 887 |
| MunI | CAATTG | 1 cut(s) | 282 |
| Mva1269I | GAATGC | 1 cut(s) | 366 |
| MvaI | CCWGG | 4 cut(s) | 434, 475, 699, 887 |
| MvnI | CGCG | 2 cut(s) | 1247, 2112 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 417, 1244, 2116 |
| NaeI | GCCGGC | 1 cut(s) | 439 |
| NarI | GGCGCC | 2 cut(s) | 135, 914 |
| NgoMIV | GCCGGC | 1 cut(s) | 437 |
| NlaIV | GGNNCC | 8 cut(s) | 136, 155, 344, 480, 884, 915, 986, 2049 |
| NmeAIII | GCCGAG | 1 cut(s) | 1330 |
| NmuCI | GTSAC | 4 cut(s) | 395, 852, 895, 1406 |
| NsiI | ATGCAT | 4 cut(s) | 35, 224, 1341, 1608 |
| NspI | RCATGY | 6 cut(s) | 33, 74, 222, 263, 1339, 1805 |
| OliI | CACNNNNGTG | 1 cut(s) | 1395 |
| PaeI | GCATGC | 2 cut(s) | 33, 222 |
| PaeR7I | CTCGAG | 1 cut(s) | 1534 |
| PciSI | GCTCTTC | 1 cut(s) | 1642 |
| PctI | GAATGC | 1 cut(s) | 366 |
| PdiI | GCCGGC | 1 cut(s) | 439 |
| PdmI | GAANNNNTTC | 1 cut(s) | 366 |
| PflFI | GACNNNGTC | 1 cut(s) | 1154 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2017, 2057 |
| PkrI | GCNGC | 3 cut(s) | 10, 88, 277 |
| PleI | GAGTC | 1 cut(s) | 1224 |
| PluTI | GGCGCC | 2 cut(s) | 138, 917 |
| PmaCI | CACGTG | 1 cut(s) | 455 |
| PmlI | CACGTG | 1 cut(s) | 455 |
| PpsI | GAGTC | 1 cut(s) | 1224 |
| Ppu21I | YACGTR | 1 cut(s) | 455 |
| PpuMI | RGGWCCY | 1 cut(s) | 882 |
| PsiI | TTATAA | 2 cut(s) | 488, 1667 |
| Psp5II | RGGWCCY | 1 cut(s) | 882 |
| Psp6I | CCWGG | 4 cut(s) | 432, 473, 697, 885 |
| PspCI | CACGTG | 1 cut(s) | 455 |
| PspEI | GGTNACC | 1 cut(s) | 395 |
| PspGI | CCWGG | 4 cut(s) | 432, 473, 697, 885 |
| PspN4I | GGNNCC | 8 cut(s) | 136, 155, 344, 480, 884, 915, 986, 2049 |
| PspPI | GGNCC | 2 cut(s) | 882, 2048 |
| PspPPI | RGGWCCY | 1 cut(s) | 882 |
| PsyI | GACNNNGTC | 1 cut(s) | 1154 |
| RsaI | GTAC | 5 cut(s) | 480, 539, 1376, 1800, 1890 |
| RsaNI | GTAC | 5 cut(s) | 479, 538, 1375, 1799, 1889 |
| RseI | CAYNNNNRTG | 5 cut(s) | 353, 561, 764, 848, 1395 |
| SapI | GCTCTTC | 1 cut(s) | 1642 |
| SaqAI | TTAA | 2 cut(s) | 1601, 2124 |
| SatI | GCNGC | 3 cut(s) | 9, 87, 276 |
| Sau96I | GGNCC | 2 cut(s) | 882, 2048 |
| ScaI | AGTACT | 2 cut(s) | 1376, 1890 |
| SchI | GAGTC | 1 cut(s) | 1225 |
| ScrFI | CCNGG | 4 cut(s) | 434, 475, 699, 887 |
| SduI | GDGCHC | 1 cut(s) | 558 |
| SfaNI | GCATC | 5 cut(s) | 206, 450, 801, 1353, 2139 |
| SfcI | CTRYAG | 1 cut(s) | 1836 |
| SfoI | GGCGCC | 2 cut(s) | 136, 915 |
| Sfr274I | CTCGAG | 1 cut(s) | 1534 |
| SinI | GGWCC | 2 cut(s) | 882, 2048 |
| SlaI | CTCGAG | 1 cut(s) | 1534 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 353, 561, 764, 848, 1395 |
| SmlI | CTYRAG | 3 cut(s) | 1534, 1884, 2141 |
| SmoI | CTYRAG | 3 cut(s) | 1534, 1884, 2141 |
| SphI | GCATGC | 2 cut(s) | 33, 222 |
| Sse9I | AATT | 9 cut(s) | 282, 573, 627, 1086, 1290, 1307, 1316, 1477, 2053 |
| SsiI | CCGC | 2 cut(s) | 1245, 1805 |
| SspDI | GGCGCC | 2 cut(s) | 134, 913 |
| SspMI | CTAG | 4 cut(s) | 510, 708, 1128, 1137 |
| StyD4I | CCNGG | 4 cut(s) | 432, 473, 697, 885 |
| StyI | CCWWGG | 1 cut(s) | 130 |
| TaaI | ACNGT | 1 cut(s) | 820 |
| TaiI | ACGT | 1 cut(s) | 457 |
| TaqI | TCGA | 2 cut(s) | 1535, 1908 |
| TasI | AATT | 9 cut(s) | 282, 573, 627, 1086, 1290, 1307, 1316, 1477, 2053 |
| TatI | WGTACW | 4 cut(s) | 537, 1374, 1798, 1888 |
| Tru1I | TTAA | 2 cut(s) | 1601, 2124 |
| Tru9I | TTAA | 2 cut(s) | 1601, 2124 |
| TscAI | CASTG | 3 cut(s) | 565, 1351, 1933 |
| TseFI | GTSAC | 4 cut(s) | 395, 852, 895, 1406 |
| TseI | GCWGC | 3 cut(s) | 8, 86, 275 |
| Tsp45I | GTSAC | 4 cut(s) | 395, 852, 895, 1406 |
| TspDTI | ATGAA | 5 cut(s) | 710, 1014, 1329, 1691, 1836 |
| TspGWI | ACGGA | 2 cut(s) | 110, 299 |
| TspRI | CASTG | 3 cut(s) | 565, 1351, 1933 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1154 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2017, 2057 |
| VpaK11BI | GGWCC | 2 cut(s) | 882, 2048 |
| XapI | RAATTY | 6 cut(s) | 627, 1086, 1290, 1307, 1316, 1477 |
| XbaI | TCTAGA | 1 cut(s) | 509 |
| XceI | RCATGY | 6 cut(s) | 33, 74, 222, 263, 1339, 1805 |
| XhoI | CTCGAG | 1 cut(s) | 1534 |
| XmnI | GAANNNNTTC | 1 cut(s) | 366 |
| XspI | CTAG | 4 cut(s) | 510, 708, 1128, 1137 |
| ZrmI | AGTACT | 2 cut(s) | 1376, 1890 |
| Zsp2I | ATGCAT | 4 cut(s) | 35, 224, 1341, 1608 |