4-coumarate--CoA ligase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 392 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 581 |
| AatII | GACGTC | 1 cut(s) | 243 |
| AccB1I | GGYRCC | 1 cut(s) | 1366 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 517 |
| AccBSI | CCGCTC | 1 cut(s) | 334 |
| AccII | CGCG | 1 cut(s) | 134 |
| AciI | CCGC | 7 cut(s) | 50, 53, 132, 332, 1079, 1418, 1633 |
| AclWI | GGATC | 5 cut(s) | 460, 493, 541, 675, 684 |
| AcoI | YGGCCR | 1 cut(s) | 47 |
| AcsI | RAATTY | 1 cut(s) | 643 |
| AcuI | CTGAAG | 5 cut(s) | 85, 582, 1175, 1464, 1521 |
| AcyI | GRCGYC | 1 cut(s) | 240 |
| AdeI | CACNNNGTG | 1 cut(s) | 715 |
| AfaI | GTAC | 5 cut(s) | 593, 604, 1272, 1476, 1480 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 517 |
| AgsI | TTSAA | 7 cut(s) | 181, 940, 1101, 1127, 1225, 1382, 1546 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 148 |
| AjnI | CCWGG | 2 cut(s) | 195, 1359 |
| AleI | CACNNNNGTG | 1 cut(s) | 865 |
| Alw21I | GWGCWC | 4 cut(s) | 408, 1073, 1341, 1620 |
| Alw26I | GTCTC | 8 cut(s) | 63, 94, 205, 290, 663, 805, 1089, 1241 |
| AlwI | GGATC | 5 cut(s) | 460, 493, 541, 675, 684 |
| Ama87I | CYCGRG | 1 cut(s) | 821 |
| AoxI | GGCC | 4 cut(s) | 47, 424, 1199, 1236 |
| ApeKI | GCWGC | 4 cut(s) | 775, 778, 959, 1387 |
| ApoI | RAATTY | 1 cut(s) | 643 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1057 |
| AspLEI | GCGC | 2 cut(s) | 136, 311 |
| AspS9I | GGNCC | 3 cut(s) | 424, 1199, 1456 |
| AsuC2I | CCSGG | 2 cut(s) | 368, 401 |
| AsuHPI | GGTGA | 4 cut(s) | 757, 868, 1391, 1463 |
| AsuII | TTCGAA | 1 cut(s) | 502 |
| AvaI | CYCGRG | 1 cut(s) | 821 |
| AvaII | GGWCC | 1 cut(s) | 1456 |
| BanI | GGYRCC | 1 cut(s) | 1366 |
| BanII | GRGCYC | 3 cut(s) | 408, 1341, 1620 |
| BbsI | GAAGAC | 1 cut(s) | 1308 |
| Bbv12I | GWGCWC | 4 cut(s) | 408, 1073, 1341, 1620 |
| BbvI | GCAGC | 4 cut(s) | 765, 787, 971, 1374 |
| BccI | CCATC | 6 cut(s) | 20, 559, 658, 799, 829, 976 |
| BceAI | ACGGC | 1 cut(s) | 160 |
| BciT130I | CCWGG | 2 cut(s) | 197, 1361 |
| BciVI | GTATCC | 1 cut(s) | 1283 |
| BcnI | CCSGG | 2 cut(s) | 368, 401 |
| BcoDI | GTCTC | 8 cut(s) | 63, 94, 205, 290, 663, 805, 1089, 1241 |
| BfaI | CTAG | 3 cut(s) | 1076, 1391, 1527 |
| BfmI | CTRYAG | 3 cut(s) | 779, 882, 1251 |
| BfoI | RGCGCY | 1 cut(s) | 312 |
| BfuI | GTATCC | 1 cut(s) | 1283 |
| BglII | AGATCT | 1 cut(s) | 1282 |
| BisI | GCNGC | 6 cut(s) | 50, 53, 776, 779, 960, 1388 |
| BlsI | GCNGC | 6 cut(s) | 51, 54, 777, 780, 961, 1389 |
| Bme1390I | CCNGG | 4 cut(s) | 197, 368, 401, 1361 |
| Bme18I | GGWCC | 1 cut(s) | 1456 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 148 |
| BmeT110I | CYCGRG | 1 cut(s) | 821 |
| BmgT120I | GGNCC | 3 cut(s) | 424, 1199, 1456 |
| BmiI | GGNNCC | 3 cut(s) | 1200, 1368, 1458 |
| BmrFI | CCNGG | 4 cut(s) | 197, 368, 401, 1361 |
| BmrI | ACTGGG | 1 cut(s) | 1224 |
| BmsI | GCATC | 5 cut(s) | 696, 1405, 1429, 1547, 1601 |
| BmuI | ACTGGG | 1 cut(s) | 1224 |
| BpiI | GAAGAC | 1 cut(s) | 1308 |
| BpmI | CTGGAG | 1 cut(s) | 1140 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1023 |
| Bpu14I | TTCGAA | 1 cut(s) | 502 |
| BpuEI | CTTGAG | 1 cut(s) | 993 |
| BpuMI | CCSGG | 2 cut(s) | 368, 401 |
| Bsa29I | ATCGAT | 1 cut(s) | 679 |
| BsaAI | YACGTR | 1 cut(s) | 1478 |
| BsaHI | GRCGYC | 1 cut(s) | 240 |
| BsaI | GGTCTC | 5 cut(s) | 63, 94, 205, 805, 1089 |
| BsaJI | CCNNGG | 1 cut(s) | 205 |
| BsaWI | WCCGGW | 1 cut(s) | 36 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 98, 128 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 517 |
| Bse1I | ACTGG | 3 cut(s) | 1099, 1108, 1219 |
| Bse3DI | GCAATG | 2 cut(s) | 648, 796 |
| BseBI | CCWGG | 2 cut(s) | 197, 1361 |
| BseCI | ATCGAT | 1 cut(s) | 679 |
| BseDI | CCNNGG | 1 cut(s) | 205 |
| BseGI | GGATG | 1 cut(s) | 1425 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 517 |
| BseMI | GCAATG | 2 cut(s) | 648, 796 |
| BseMII | CTCAG | 2 cut(s) | 1037, 1621 |
| BseNI | ACTGG | 3 cut(s) | 1099, 1108, 1219 |
| BseRI | GAGGAG | 3 cut(s) | 100, 103, 1001 |
| BseX3I | CGGCCG | 1 cut(s) | 47 |
| BseXI | GCAGC | 4 cut(s) | 765, 787, 971, 1374 |
| Bsh1236I | CGCG | 1 cut(s) | 134 |
| Bsh1285I | CGRYCG | 2 cut(s) | 50, 1637 |
| BshFI | GGCC | 4 cut(s) | 49, 426, 1201, 1238 |
| BshNI | GGYRCC | 1 cut(s) | 1366 |
| BshVI | ATCGAT | 1 cut(s) | 679 |
| BsiEI | CGRYCG | 2 cut(s) | 50, 1637 |
| BsiHKAI | GWGCWC | 4 cut(s) | 408, 1073, 1341, 1620 |
| BsiHKCI | CYCGRG | 1 cut(s) | 821 |
| BsiSI | CCGG | 4 cut(s) | 37, 306, 367, 401 |
| BsiWI | CGTACG | 1 cut(s) | 1478 |
| BslFI | GGGAC | 3 cut(s) | 383, 383, 1442 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 517 |
| BsmAI | GTCTC | 8 cut(s) | 63, 94, 205, 290, 663, 805, 1089, 1241 |
| BsmBI | CGTCTC | 1 cut(s) | 663 |
| BsmFI | GGGAC | 3 cut(s) | 383, 383, 1442 |
| BsnI | GGCC | 4 cut(s) | 49, 426, 1201, 1238 |
| Bso31I | GGTCTC | 5 cut(s) | 63, 94, 205, 805, 1089 |
| BsoBI | CYCGRG | 1 cut(s) | 821 |
| Bsp119I | TTCGAA | 1 cut(s) | 502 |
| Bsp1286I | GDGCHC | 4 cut(s) | 408, 1073, 1341, 1620 |
| Bsp143I | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| Bsp19I | CCATGG | 1 cut(s) | 205 |
| BspACI | CCGC | 7 cut(s) | 50, 53, 132, 332, 1079, 1418, 1633 |
| BspANI | GGCC | 4 cut(s) | 49, 426, 1201, 1238 |
| BspCNI | CTCAG | 2 cut(s) | 1036, 1620 |
| BspDI | ATCGAT | 1 cut(s) | 679 |
| BspFNI | CGCG | 1 cut(s) | 134 |
| BspLI | GGNNCC | 3 cut(s) | 1200, 1368, 1458 |
| BspMAI | CTGCAG | 1 cut(s) | 783 |
| BspPI | GGATC | 5 cut(s) | 460, 493, 541, 675, 684 |
| BspT104I | TTCGAA | 1 cut(s) | 502 |
| BspT107I | GGYRCC | 1 cut(s) | 1366 |
| BspTNI | GGTCTC | 5 cut(s) | 63, 94, 205, 805, 1089 |
| BsrBI | CCGCTC | 1 cut(s) | 334 |
| BsrDI | GCAATG | 2 cut(s) | 648, 796 |
| BsrI | ACTGG | 3 cut(s) | 1099, 1108, 1219 |
| BssECI | CCNNGG | 1 cut(s) | 205 |
| BssMI | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| BssNI | GRCGYC | 1 cut(s) | 240 |
| BssT1I | CCWWGG | 1 cut(s) | 205 |
| Bst2UI | CCWGG | 2 cut(s) | 197, 1361 |
| Bst4CI | ACNGT | 6 cut(s) | 343, 397, 442, 908, 1176, 1577 |
| Bst6I | CTCTTC | 4 cut(s) | 104, 293, 601, 1606 |
| BstACI | GRCGYC | 1 cut(s) | 240 |
| BstBAI | YACGTR | 1 cut(s) | 1478 |
| BstBI | TTCGAA | 1 cut(s) | 502 |
| BstC8I | GCNNGC | 4 cut(s) | 132, 756, 891, 1109 |
| BstDEI | CTNAG | 3 cut(s) | 448, 1023, 1607 |
| BstDSI | CCRYGG | 1 cut(s) | 205 |
| BstF5I | GGATG | 1 cut(s) | 1425 |
| BstFNI | CGCG | 1 cut(s) | 134 |
| BstH2I | RGCGCY | 1 cut(s) | 312 |
| BstHHI | GCGC | 2 cut(s) | 136, 311 |
| BstKTI | GATC | 8 cut(s) | 455, 488, 536, 679, 683, 820, 1285, 1327 |
| BstMAI | GTCTC | 8 cut(s) | 63, 94, 205, 290, 663, 805, 1089, 1241 |
| BstMBI | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| BstMCI | CGRYCG | 2 cut(s) | 50, 1637 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 27, 308, 358 |
| BstNI | CCWGG | 2 cut(s) | 197, 1361 |
| BstNSI | RCATGY | 1 cut(s) | 1111 |
| BstSCI | CCNGG | 4 cut(s) | 195, 366, 399, 1359 |
| BstSFI | CTRYAG | 3 cut(s) | 779, 882, 1251 |
| BstSNI | TACGTA | 1 cut(s) | 1478 |
| BstUI | CGCG | 1 cut(s) | 134 |
| BstV1I | GCAGC | 4 cut(s) | 765, 787, 971, 1374 |
| BstV2I | GAAGAC | 1 cut(s) | 1308 |
| BstX2I | RGATCY | 2 cut(s) | 533, 1282 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 749, 1177 |
| BstYI | RGATCY | 2 cut(s) | 533, 1282 |
| BstZI | CGGCCG | 1 cut(s) | 47 |
| Bsu15I | ATCGAT | 1 cut(s) | 679 |
| BsuI | GTATCC | 1 cut(s) | 1283 |
| BsuRI | GGCC | 4 cut(s) | 49, 426, 1201, 1238 |
| BsuTUI | ATCGAT | 1 cut(s) | 679 |
| BtgI | CCRYGG | 1 cut(s) | 205 |
| BtsCI | GGATG | 1 cut(s) | 1425 |
| BtsIMutI | CAGTG | 5 cut(s) | 339, 447, 913, 1151, 1582 |
| Cac8I | GCNNGC | 4 cut(s) | 132, 756, 891, 1109 |
| CfoI | GCGC | 2 cut(s) | 136, 311 |
| Cfr13I | GGNCC | 3 cut(s) | 424, 1199, 1456 |
| ClaI | ATCGAT | 1 cut(s) | 679 |
| CseI | GACGC | 1 cut(s) | 291 |
| Csp6I | GTAC | 5 cut(s) | 592, 603, 1271, 1475, 1479 |
| CviAII | CATG | 5 cut(s) | 206, 512, 713, 743, 1108 |
| CviQI | GTAC | 5 cut(s) | 592, 603, 1271, 1475, 1479 |
| DdeI | CTNAG | 3 cut(s) | 448, 1023, 1607 |
| DpnI | GATC | 8 cut(s) | 454, 487, 535, 678, 682, 819, 1284, 1326 |
| DpnII | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| DraIII | CACNNNGTG | 1 cut(s) | 715 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 581 |
| DriI | GACNNNNNGTC | 1 cut(s) | 148 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 581 |
| EaeI | YGGCCR | 1 cut(s) | 47 |
| EagI | CGGCCG | 1 cut(s) | 47 |
| Eam1104I | CTCTTC | 4 cut(s) | 104, 293, 601, 1606 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 148 |
| EarI | CTCTTC | 4 cut(s) | 104, 293, 601, 1606 |
| Ecl136II | GAGCTC | 3 cut(s) | 406, 1339, 1618 |
| EclXI | CGGCCG | 1 cut(s) | 47 |
| Eco105I | TACGTA | 1 cut(s) | 1478 |
| Eco130I | CCWWGG | 1 cut(s) | 205 |
| Eco24I | GRGCYC | 3 cut(s) | 408, 1341, 1620 |
| Eco31I | GGTCTC | 5 cut(s) | 63, 94, 205, 805, 1089 |
| Eco47I | GGWCC | 1 cut(s) | 1456 |
| Eco52I | CGGCCG | 1 cut(s) | 47 |
| Eco53kI | GAGCTC | 3 cut(s) | 406, 1339, 1618 |
| Eco57I | CTGAAG | 5 cut(s) | 85, 582, 1175, 1464, 1521 |
| Eco88I | CYCGRG | 1 cut(s) | 821 |
| EcoICRI | GAGCTC | 3 cut(s) | 406, 1339, 1618 |
| EcoO109I | RGGNCCY | 3 cut(s) | 424, 1199, 1456 |
| EcoRII | CCWGG | 2 cut(s) | 195, 1359 |
| EcoT14I | CCWWGG | 1 cut(s) | 205 |
| EcoT38I | GRGCYC | 3 cut(s) | 408, 1341, 1620 |
| ErhI | CCWWGG | 1 cut(s) | 205 |
| Esp3I | CGTCTC | 1 cut(s) | 663 |
| FaeI | CATG | 5 cut(s) | 209, 515, 716, 746, 1111 |
| FaqI | GGGAC | 3 cut(s) | 383, 383, 1442 |
| FatI | CATG | 5 cut(s) | 205, 511, 712, 742, 1107 |
| FauI | CCCGC | 2 cut(s) | 139, 1072 |
| Fnu4HI | GCNGC | 6 cut(s) | 50, 53, 776, 779, 960, 1388 |
| FokI | GGATG | 1 cut(s) | 1412 |
| FriOI | GRGCYC | 3 cut(s) | 408, 1341, 1620 |
| Fsp4HI | GCNGC | 6 cut(s) | 50, 53, 776, 779, 960, 1388 |
| FspBI | CTAG | 3 cut(s) | 1076, 1391, 1527 |
| GlaI | GCGC | 2 cut(s) | 135, 310 |
| GluI | GCNGC | 6 cut(s) | 50, 53, 776, 779, 960, 1388 |
| GsuI | CTGGAG | 1 cut(s) | 1140 |
| HaeII | RGCGCY | 1 cut(s) | 312 |
| HaeIII | GGCC | 4 cut(s) | 49, 426, 1201, 1238 |
| HapII | CCGG | 4 cut(s) | 37, 306, 367, 401 |
| HgaI | GACGC | 1 cut(s) | 291 |
| HhaI | GCGC | 2 cut(s) | 136, 311 |
| Hin1I | GRCGYC | 1 cut(s) | 240 |
| Hin1II | CATG | 5 cut(s) | 209, 515, 716, 746, 1111 |
| Hin6I | GCGC | 2 cut(s) | 134, 309 |
| HinP1I | GCGC | 2 cut(s) | 134, 309 |
| HincII | GTYRAC | 1 cut(s) | 559 |
| HindII | GTYRAC | 1 cut(s) | 559 |
| HinfI | GANTC | 6 cut(s) | 149, 371, 499, 524, 813, 1329 |
| HpaI | GTTAAC | 1 cut(s) | 559 |
| HpaII | CCGG | 4 cut(s) | 37, 306, 367, 401 |
| HphI | GGTGA | 4 cut(s) | 757, 868, 1391, 1463 |
| Hpy166II | GTNNAC | 3 cut(s) | 559, 1273, 1475 |
| Hpy188I | TCNGA | 7 cut(s) | 104, 157, 349, 544, 663, 1134, 1610 |
| Hpy8I | GTNNAC | 3 cut(s) | 559, 1273, 1475 |
| Hpy99I | CGWCG | 4 cut(s) | 146, 242, 245, 937 |
| HpyAV | CCTTC | 4 cut(s) | 103, 178, 778, 1376 |
| HpyCH4III | ACNGT | 6 cut(s) | 343, 397, 442, 908, 1176, 1577 |
| HpyCH4IV | ACGT | 3 cut(s) | 240, 480, 1477 |
| HpyCH4V | TGCA | 4 cut(s) | 709, 781, 1466, 1538 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 27, 308, 358 |
| HpyF3I | CTNAG | 3 cut(s) | 448, 1023, 1607 |
| HpySE526I | ACGT | 3 cut(s) | 240, 480, 1477 |
| Hsp92I | GRCGYC | 1 cut(s) | 240 |
| Hsp92II | CATG | 5 cut(s) | 209, 515, 716, 746, 1111 |
| HspAI | GCGC | 2 cut(s) | 134, 309 |
| KspAI | GTTAAC | 1 cut(s) | 559 |
| Kzo9I | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| LmnI | GCTCC | 2 cut(s) | 403, 967 |
| Lsp1109I | GCAGC | 4 cut(s) | 765, 787, 971, 1374 |
| LweI | GCATC | 5 cut(s) | 696, 1405, 1429, 1547, 1601 |
| MaeI | CTAG | 3 cut(s) | 1076, 1391, 1527 |
| MaeII | ACGT | 3 cut(s) | 240, 480, 1477 |
| MaeIII | GTNAC | 3 cut(s) | 39, 667, 856 |
| MalI | GATC | 8 cut(s) | 454, 487, 535, 678, 682, 819, 1284, 1326 |
| MbiI | CCGCTC | 1 cut(s) | 334 |
| MboI | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| MboII | GAAGA | 8 cut(s) | 91, 280, 588, 838, 1313, 1514, 1517, 1623 |
| MflI | RGATCY | 2 cut(s) | 533, 1282 |
| MhlI | GDGCHC | 4 cut(s) | 408, 1073, 1341, 1620 |
| MluCI | AATT | 5 cut(s) | 272, 643, 759, 999, 1053 |
| MlyI | GAGTC | 3 cut(s) | 158, 365, 533 |
| MmeI | TCCRAC | 1 cut(s) | 463 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1057 |
| MseI | TTAA | 7 cut(s) | 212, 429, 558, 564, 915, 924, 1344 |
| MslI | CAYNNNNRTG | 1 cut(s) | 865 |
| MspA1I | CMGCKG | 3 cut(s) | 581, 778, 1373 |
| MspI | CCGG | 4 cut(s) | 37, 306, 367, 401 |
| MspR9I | CCNGG | 4 cut(s) | 197, 368, 401, 1361 |
| MvaI | CCWGG | 2 cut(s) | 197, 1361 |
| MvnI | CGCG | 1 cut(s) | 134 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 27, 308, 358 |
| NciI | CCSGG | 2 cut(s) | 368, 401 |
| NcoI | CCATGG | 1 cut(s) | 205 |
| NdeII | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| NlaIII | CATG | 5 cut(s) | 209, 515, 716, 746, 1111 |
| NlaIV | GGNNCC | 3 cut(s) | 1200, 1368, 1458 |
| NmuCI | GTSAC | 3 cut(s) | 39, 667, 856 |
| NspI | RCATGY | 1 cut(s) | 1111 |
| NspV | TTCGAA | 1 cut(s) | 502 |
| OliI | CACNNNNGTG | 1 cut(s) | 865 |
| PaeI | GCATGC | 1 cut(s) | 1111 |
| PaeR7I | CTCGAG | 1 cut(s) | 821 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1057 |
| PfeI | GAWTC | 3 cut(s) | 499, 813, 1329 |
| Pfl23II | CGTACG | 1 cut(s) | 1478 |
| PflFI | GACNNNGTC | 1 cut(s) | 242 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 517 |
| PfoI | TCCNGGA | 2 cut(s) | 366, 399 |
| PkrI | GCNGC | 6 cut(s) | 51, 54, 777, 780, 961, 1389 |
| PleI | GAGTC | 3 cut(s) | 157, 365, 532 |
| PpsI | GAGTC | 3 cut(s) | 157, 365, 532 |
| Ppu21I | YACGTR | 1 cut(s) | 1478 |
| PpuMI | RGGWCCY | 1 cut(s) | 1456 |
| PsiI | TTATAA | 1 cut(s) | 392 |
| Psp124BI | GAGCTC | 3 cut(s) | 408, 1341, 1620 |
| Psp5II | RGGWCCY | 1 cut(s) | 1456 |
| Psp6I | CCWGG | 2 cut(s) | 195, 1359 |
| PspGI | CCWGG | 2 cut(s) | 195, 1359 |
| PspLI | CGTACG | 1 cut(s) | 1478 |
| PspN4I | GGNNCC | 3 cut(s) | 1200, 1368, 1458 |
| PspPI | GGNCC | 3 cut(s) | 424, 1199, 1456 |
| PspPPI | RGGWCCY | 1 cut(s) | 1456 |
| PstI | CTGCAG | 1 cut(s) | 783 |
| PsuI | RGATCY | 2 cut(s) | 533, 1282 |
| PsyI | GACNNNGTC | 1 cut(s) | 242 |
| PvuII | CAGCTG | 3 cut(s) | 581, 778, 1373 |
| RsaI | GTAC | 5 cut(s) | 593, 604, 1272, 1476, 1480 |
| RsaNI | GTAC | 5 cut(s) | 592, 603, 1271, 1475, 1479 |
| RseI | CAYNNNNRTG | 1 cut(s) | 865 |
| SacI | GAGCTC | 3 cut(s) | 408, 1341, 1620 |
| SaqAI | TTAA | 7 cut(s) | 212, 429, 558, 564, 915, 924, 1344 |
| SatI | GCNGC | 6 cut(s) | 50, 53, 776, 779, 960, 1388 |
| Sau3AI | GATC | 8 cut(s) | 452, 485, 533, 676, 680, 817, 1282, 1324 |
| Sau96I | GGNCC | 3 cut(s) | 424, 1199, 1456 |
| SchI | GAGTC | 3 cut(s) | 158, 365, 533 |
| ScrFI | CCNGG | 4 cut(s) | 197, 368, 401, 1361 |
| SduI | GDGCHC | 4 cut(s) | 408, 1073, 1341, 1620 |
| SfaNI | GCATC | 5 cut(s) | 696, 1405, 1429, 1547, 1601 |
| SfcI | CTRYAG | 3 cut(s) | 779, 882, 1251 |
| Sfr274I | CTCGAG | 1 cut(s) | 821 |
| SfuI | TTCGAA | 1 cut(s) | 502 |
| SinI | GGWCC | 1 cut(s) | 1456 |
| SlaI | CTCGAG | 1 cut(s) | 821 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 865 |
| SmlI | CTYRAG | 2 cut(s) | 821, 1008 |
| SmoI | CTYRAG | 2 cut(s) | 821, 1008 |
| SnaBI | TACGTA | 1 cut(s) | 1478 |
| SphI | GCATGC | 1 cut(s) | 1111 |
| Sse9I | AATT | 5 cut(s) | 272, 643, 759, 999, 1053 |
| SsiI | CCGC | 7 cut(s) | 50, 53, 132, 332, 1079, 1418, 1633 |
| SspI | AATATT | 1 cut(s) | 1410 |
| SspMI | CTAG | 3 cut(s) | 1076, 1391, 1527 |
| SstI | GAGCTC | 3 cut(s) | 408, 1341, 1620 |
| StyD4I | CCNGG | 4 cut(s) | 195, 366, 399, 1359 |
| StyI | CCWWGG | 1 cut(s) | 205 |
| TaaI | ACNGT | 6 cut(s) | 343, 397, 442, 908, 1176, 1577 |
| TaiI | ACGT | 3 cut(s) | 243, 483, 1480 |
| TaqII | GACCGA | 1 cut(s) | 587 |
| TasI | AATT | 5 cut(s) | 272, 643, 759, 999, 1053 |
| TatI | WGTACW | 1 cut(s) | 591 |
| TauI | GCSGC | 2 cut(s) | 52, 55 |
| TfiI | GAWTC | 3 cut(s) | 499, 813, 1329 |
| Tru1I | TTAA | 7 cut(s) | 212, 429, 558, 564, 915, 924, 1344 |
| Tru9I | TTAA | 7 cut(s) | 212, 429, 558, 564, 915, 924, 1344 |
| TscAI | CASTG | 5 cut(s) | 346, 447, 913, 1158, 1582 |
| TseFI | GTSAC | 3 cut(s) | 39, 667, 856 |
| TseI | GCWGC | 4 cut(s) | 775, 778, 959, 1387 |
| Tsp45I | GTSAC | 3 cut(s) | 39, 667, 856 |
| TspDTI | ATGAA | 3 cut(s) | 637, 1314, 1558 |
| TspGWI | ACGGA | 1 cut(s) | 1320 |
| TspRI | CASTG | 5 cut(s) | 346, 447, 913, 1158, 1582 |
| Tth111I | GACNNNGTC | 1 cut(s) | 242 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 517 |
| VpaK11BI | GGWCC | 1 cut(s) | 1456 |
| XapI | RAATTY | 1 cut(s) | 643 |
| XceI | RCATGY | 1 cut(s) | 1111 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 203 |
| XhoI | CTCGAG | 1 cut(s) | 821 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1057 |
| XspI | CTAG | 3 cut(s) | 1076, 1391, 1527 |
| ZraI | GACGTC | 1 cut(s) | 241 |