pycom15g37190

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000800
Physical Location & Seq
Forward (+)
37074869 .. 37091892
17024 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6894 bp
ATGGGAATTACAGTCTTGGCGCGCCCTAGGCCCAGAAAAAGCCAGACATTTCTTACTCTCTCTCCACAGGCCATGGAGGAGACCGCCATCGTTTCAAAACTTGAAGCCTCCAACTCCGCCGGCCTTCATCCCCTCGTCGCCGCCTACCTTCAGCCCTTCACTGAACTCCAGAACCCCAAAAAAACAAAAAAACCAACCAAATCCCAAGACCACCAAACCCTCCTCCGCACGCTCGCCAAGAAATTCCTCCCCTTCCTCAACCGCTCGCTATCCCTTCTCCCCAAACGCCTCGCCGACCCCTCAAAGTTAGATGACAAATTTGCCCTAGAACTCTTCGATATTTACCGGCTCTGCCTCGACTGCCTGGCCGCCGTGTCCTCTGTGTTGTCCGCTTCACCGTACTCGTTTCACTACCCGAGGGTGAGGATGGTGAGTTGCCTCGTGTCATGTGGGCGGTACAAGGATGCGGAAAGTGAGGGGTTTAGGGTTTTGGAGAGCCTTAAGGCGATTGAATGTGGATCGAAGAAATCGGTGAAATCGGATCGGAGATTTGTGCCGGATGTGGAGAAGGGGGGTGGAGATAAAGATTTTGGGTCTTTGGTGGGTGAAATTGTGGTGACGCTTGTGAAATGTGCGGCCATGAGTCAGAGTAAGGACAGTGAGGTGTTTGAGCGGGTGCTTTGTTTGGCTGAGGAGGTCATGCCGTGGTTCCGGGTATTAGATGCAAGCACATGTGAGAAGTTGCACAGGAATCTGGTTGCCTATCTGAGTAGGTGTACTCAATTTTTAGTTGGGGAGCTATCGGTGTTCAATGGGGATCTAGTGCAAAAGTTCTGCATTGTAACCATGACCGAGTATGCTAAGTCGCCAATGAAAGACACAATGTTCAAGTTTGCCCGTACAATATGTTCATCTTTATTCTTGTTTCAAGAGGATACATTCACACTCACTCGCATCTTATTTTGCTTGTTGGACTCCCTTGTCCACGAATGTAAGGCTGAAGTGGAAAATACAGGAAAAGAGTTAGTTGAACTTATTGCTTATTGTGCCAAGAAATGTCAAAATACAAACACAAACCTTTGCGGTATTATTGGATCTCATTTAAATAAGTTAGCAGGTGATTTCCATCAGGCTGGAACACCTTTTCAGTTAATTCTGAGGGTTTATGCCACTGGATTGCACTTTGTTGATCGTAGTATGAAGTCAAAAGTTGGTCATTTTCAATCCTTTGAAGGTGCAATTAGAGTTTTGCTTGATGATGGGGACACAGTGAATCGCTTGTCCGGTTTGCTTGGTTCATTGAGAAGTTATTTCCAAATTGGCTGCAATGACGACTCTTTGTTATCCAATTCGCAATTGAGTTCGGACAGTGGGGACTCATTGATTCAAATGCAGAAGGATAGAAAGAACTATCTTCTGTCTTATTTTAATGCATTGAAATTCTTATGCCAGCCGCTCACAGAGTTTGTAAATTCAGGAAGGAAACAGATTATTACTAATAATGAAGCTGCTTCTGTTTCTACTGAAGTGTGCCATATCCAGGGTTGTCATTCTTGTGATATAATGCTGAAATGGTCCCAGTTATTTGTTTTCTCATTAGTGAAATGTTTATTTCTTATGACTCTTGTACAGCACAATCTTTATCTGCGCAAACTAGGTAATTGGCTTGCCTTTTTCTTATTTCAGAAGAGTGTGCAAATACTTGAAAATGTCATCACCAGTGTTTGGATTCAACCTCATGGTCTAAAGCATCTGTATGTCTCTCTGTACAATATCGGGGTACATTTGTACCGGAACAAGGAGCTAAAAGAGGTATTCTTTTACAGAATGAATATTTATGACTGGATGGCATATGGTGTAGCATCACAGGCATTGAACTTATGTTGCAAGGCATCATGGACTCGTGTTATACGTCTTTGTGAGATGTTTGTACACAAACAAGAGGCGTCCGAGGTTGATCTGTCAGAAGATGCCATTTTAGATTTCTATAATGAGTGCTGTACGAGAAGTGCTTTTCTTTTGGACGTTCTTAATGAATTGCAGAGTTATGATGCGAAAAGAACTTTATTAGAGAGCCTTGAAAATTGGTCCATTGCTGCAAACTTGTTTGGGAGGCTGCCAGGTCCTATGGCTGTGGTGAAGCAGTGGGTTAAGATGGAATGTAGACGTTACAAGGATGTGAATGTTGAAGATGATGCTCCAACCTTATACAGTTTGTTATTGTCTTATAAGAAAGTCCCAAAGAAGATAAACGAAACCGTTTTGGAACAGGTTGGAACATTTTGTATTTCTTTGCAGTTGGAGTTCATTATATATATATATATATATATCCCTAAGACTTTAGGTCGCACTTGTACTAACTTTTCTCACGTCTACATTTCTATCTTGAAATATCAGGAACTTCTTGCTTATGAGGGAATGACTGCTGTGAACCCTAAGTTTTGTCAGAAAATGCAAATGAAAATTATAGATTTCCTGCTGAAAGATGTGTATGTCACACCAAATAGTTGGTTACAAAAATCAAGAATTTTGTTGAAAAAGGGAAGGGCATTAAGGCTTTCTGGAAGCAATGGTCTGAAGGACTGTTTTCAGTGTTTATCGGATGCAATATGTTTACTTAGTGAGATGTATGATGAAACCTGTACCCATGAAATTTCTCCTTGCCATCAATTAGCTGTGGCATATTGCTTACGTGCACTTTCTACCCAGGAAGCTGAACCCAACTCAAAGCGAGTGCTTGAAGATATCAGTGCTGCCATTAATCTATGGTTGGGCATTTCTACTCCTGATAATTGCTCTCCGGCTGACAAGTGCTCCATGTTGTCTGAAAATACTATGTTGCTACTTTACAATGTCATTGATTTGTTATCCATCAAGGGTTGCATGGACTTTCATAATGATATACATAAGCTTATGATTAGATTATTTAAATGGAGGAATGTCCCATTAGAGAAGTGCGTGGCCAGATTTTGGGAATGTAGGAGGATTAGCCATGCCCTTTGTGCCTCACCTGTAAATGAGACATTCATTATGAACTTATCGGATCATTGTGGTGAACTTTCAAAGTCTAATGCTTTTTGGATAGATTCTCTAAAGGATTCCAGACCATTACTATTAGTGTTCCAACATAGTTTCTCATTTCTGTTTCCAAACTTTTCTCGGGGCCCATGGAATCATCAAAGCTTGTTTCGATCAGATATCACAATTGATGAAGTTAAGGAAGCTGCTTTTGGACTTATATCACAGGCTCCTGTATCGACTTGGTCTGCTTACATAGCTGGATATCTCTACTATGATTTATCGGAAAGACTTGTTTCAAATGGACGGTTAATTGAGGCTCTCTCATATGCAAAAGAAGCTCACAACTTACGTGCTAAACTATTTGGAGGGAAATTTATGTTCTCTTCTGAGCGACAGCCCAAAAAGTACAATGAAGGGGGTATTTGTCAGGAGTTAACATATAGCATTCATGATATGCATATGCAAAGATCAGTTGCTAGTGAAGTTTGGCTTTTTGATACTAGTTCATGTGACCTGGAAAGCTATTATCTGAGTCCATGGAATGCACTTCAATGTTATCTTGAGAGCACTCTTCAGGTAGGGGTTATCCTTGAAATAATTGGTAAAGGAGCTGAGGCCGAAGGTTTTTTACAATTTGGAAAAGCTTTTTCCTGCTCACAGAGCTTGCCACTATTTACAATTGTTTTTTCTACTGTCTTGGGAAAGCTTTACCACAAGCAGCAACTTTGGGATTTGGCGGAAAAGGAACTGCAAAGTGCCAAGCAATATTTTGGGGCTTGCAGCACAGATCTTTCTTGCATGAAATGCAGATTGTTGCTGGAAGCAACTGTTAATCAGAATCTTGGTGATTTATATCAAAGCATTTCTGAAAATACAAGAAGTACATCGTCAGATAAGTTGTCGCATGCTGAAAACCTGTACAAATCAGCCATTGCCATACTAAATCTTTCTGAGTGGAAAAATTCTGTTAGTTGTCCAGAAGAAGAGTGTGTTGAATGGACAATGCCTGGAAAAGCTAGTTTTAAAGACGTTGGATATTGTGTTAGCAGTATATATGCTGTTTCTGAAGAAAAACAGCGGGATAATAGAAAACCCACTAAGGAGGGCCTGAAAAGCAAGATGGATGCTAAAAAATGTAAAAAGACCAAAAATGCACCAAAACCGGTCGTGAAGAATCAGGTTTCAGTACCTGAGCACAATTTGAGGGTAACTCGGTCTAGATATCAATCTTCTCAGAACCAAAGCATAAGTGGAAATGGCATCGTCCAACTTGGTCCTTCAAAACATCTGCAAGGAAAAAGTGAGCATGATAGCCCTGATACTTTTAGCAAGAGGGAATTTCTTTTGGATTTAAAAAGCTGTGAGGTTGCTTTTGGGAGCAATGTAACTTGCATTTGCAACCAAATGAGGTGTTGGCAATGTCTTCCTATGGAAGTTATGGAATCTGGGTTAGTGACAGACTTAGTACATTTAAAATGGGAGTTTGTCCGCAGGCGTTTATCACTCAGGCTTCTCACAGGATTAGGAAAATGCTTGGAGAGTCGTGGTCAAATTCACGAGACACATGAAATTATAGTGCAAACTGTATCAGTCTTGGTCAGCCGAAACCCATTTTGTCCTATTACCTCAACTGTTCCATTGACTTCCTTGCTTGATTTAATGGGAAAGGAGATCCCCGGGGATGTGTTCTGTGTTGAACGGGCAGAAGTACTGTTGAACATAAGTTGGTTGTCTTTGAAGAGTTACTGTTCTAAGGAAACCAGGAGTATGTGCTCTGATCTGCCTCATATTCAGCTACCAAAACTAGTGTCTTGGTTGATGCTAGCTTTTGTTCTCTGCCGCGACGTTCCTGTACTTTTCCAGAAGGTTTCCAGATTGCTTGCAGCTATATTTGTGCTTTCTGCCTCAAGTGATCTTTTTTCTTTGTCATCTTCTTCTAAAACTCTCCGTGAAAACCATTGGGCTTCTTATTTCCATCAAGCTTCACTTGGCACTCATCTTAGTTGCCAATTTTTCACAAATATATCTGGGATATGTAATGTTCAGCACCTTGTAAACACTGAGGGTTCACATGTTCCTGGCTCAACTTGTTTGGGATCAGAAAAAAGGAATTTACTCAGGCTTGCACCTGAGTCTATTCAAGAACTTGAAGGATTTGTAACACTGTTTTTTGCCGGCCTTCCTTGCACAACAATTATCTGTATAAGTTTGCTCGGAAGCCCTTATGCTAGTTTCCTACAGGAGCTGTTGTCCTTTCATACTTGTGTTCATGCATGGATTCTTGTATCACGCTTGAACTTGAAGAGTCAACCTATCATTATGCTTCTACCTGTGGATTCAGTATTAGAAGGAGACTCCTCTGATGATACAAGTTCTGGTTCTGTTAGTGTTTCTGAAGGAAAGGTTGGAAAGCGTTGGTGCTGCCCATGGGGTTCCACTGTGGTTGACAGGGTAGCTCCAGAATTTAGAATGATATTGGAGGAGAGTTATTTGTCTTCTTCAATCGAAGAAGAAGATACAAAAGAGAATAGGGCGTTATGGTGGATGTGGAGAAATAAGCTTGATCGTCGCCTCTGTAAATTGTTGAAGAACTTAGAAGATTTATGGTTTGGTCCTTGGAAATATGTGCTTCTGGGAGAATCGTCAAACTGCAAGCAGCTGGACTTGGTACATAAGAAGCTGGCACGGGATTTGAAATCTAAGTGCAAAATGGACATAGATGAGAGTCTTCTGAGAGTCATTCTTGGGGGTTCCAAATATGCCTTTGAAGGAGGAGGTGCATATGTTTCACAGCTTTGTTTCAAGAAAGGTTGCTATATTGGTAAAGCTGGATGTTCCGAGGAAAATAATTGGTTGGCATCACCTAACGAATCTAATGGTTATCAGAAACTATCTGAATTGGCTTTCCAACTAATACAGGGAGCAGTGAATGAGCTTGAAGGGCTAGATACTGTAAATAGAGAGCCCATCATTCTAGTGCTGGACTTCGAGGTGCAGATGCTTCCTTGGGAGAATATACCGATACTAAGAAACCAGGAGGCTTATCGCATGCCTTCTGTTGGGAGCATCTTTGCAACGCTGGAGAAGAATTACCATCAAGATAAAGTTGCATGTAGTACTAAGAAACCAGGGGGCTTATCGCATGCCTTCTGTCAGGAGGCTTCATTCCCTTCGATTGATCCGTTGGATGCATTTTATCTCCTGAACCCAGGTGGTGATCTAGGTATCACACAAATCGAATTTGAGAAGTGGTTCAGAGATCAAAATTTGGAGGGGAAGGCCGGATGTGCACCCCCTGCTGAAGAATTGGCAGAAGCCTTGAAAAGCCATGACCTCTTTATATATATCGGCCACGGAAGTGGGGTGAACTATATTCCCATGCATCAGATTCAGAGTCTGGAAAATTGTGCTGCTACTCTTTTGATGGGATGTAGTAGTGGTTCTCTGACACTGAATGGTTGTTATGTTCCACACGGTCCCGCACTATCTTATCTGCTGGCTGGTTCTCCTGTCATTATTGGAAATTTATGGGAAGTGACAGACAAGGACATCAACCGATTTGCAAAGGCCATGCTTGATGGTTGGCTAAAAGAAAGATCGAGTTCCTCTGAGGGTTGTGCCGAATGCAGAGTAGCAGAAGAATTTGAGGCATTGAGCATTAGGGGCCGTCAAGGTATTGCCAAGAAGAAAGTCTCGAGGAAGAAATTGCCTGAAGCTTGTGAAAGTGATCCAATGATGATTTCTTGTGATCATAGGCCGAAGATTGGATCATTCGCGAGTCAAGCTCGTGAAGCCTGCAGTCTTCCTTTCTTAATTGGAGCATCACCAGTATGTTATGGTGTTCCTACGGGCATAAGGAGAAAAGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

2298

Amino Acids

258.55

Weight (kDa)

7.75

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_ESP1 PF25110 27 - 306 7.8e-114 Separase-like, TPR repeats
TPR_ESP1_2nd PF25113 318 - 497 2.3e-55 Separase-like, TPR repeats
Separin_C PF03568 1765 - 2191 1.3e-92 Separin, protease domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2229
AarI CACCTGC 1 cut(s) 1106
AasI GACNNNNNNGTC 1 cut(s) 980
Acc16I TGCGCA 1 cut(s) 1649
Acc36I ACCTGC 1 cut(s) 1106
AccB7I CCANNNNNTGG 2 cut(s) 1725, 2970
AccBSI CCGCTC 3 cut(s) 264, 673, 1456
AccI GTMKAC 2 cut(s) 2164, 2373
AccII CGCG 3 cut(s) 22, 4863, 6797
AcoI YGGCCR 4 cut(s) 366, 636, 2961, 6370
AcuI CTGAAG 9 cut(s) 134, 1020, 1545, 2597, 3578, 4107, 5436, 6342, 6753
AcyI GRCGYC 1 cut(s) 1946
AfiI CCNNNNNNNGG 6 cut(s) 1540, 1725, 1798, 2970, 6080, 6785
AflII CTTAAG 1 cut(s) 500
AflIII ACRYGT 2 cut(s) 731, 5092
AgeI ACCGGT 1 cut(s) 4183
AhdI GACNNNNNGTC 1 cut(s) 2343
AhlI ACTAGT 2 cut(s) 3521, 4825
AjiI CACGTC 1 cut(s) 2371
AjuI GAANNNNNNNTTGG 6 cut(s) 3213, 3245, 5409, 5441, 6745, 6777
AleI CACNNNNGTG 1 cut(s) 6378
Alw21I GWGCWC 6 cut(s) 2698, 2816, 3590, 4218, 4796, 6313
Alw26I GTCTC 6 cut(s) 74, 1765, 3014, 4574, 5367, 6718
Alw44I GTGCAC 2 cut(s) 2694, 6309
AlwNI CAGNNNCTG 3 cut(s) 4211, 5266, 6418
Ama87I CYCGRG 4 cut(s) 415, 3159, 4697, 6715
ApaI GGGCCC 1 cut(s) 3167
ApaLI GTGCAC 2 cut(s) 2694, 6309
ArsI GACNNNNNNTTYG 2 cut(s) 4814, 4846
AscI GGCGCGCC 1 cut(s) 20
AseI ATTAAT 1 cut(s) 2760
AsiGI ACCGGT 1 cut(s) 4183
Asp700I GAANNNNTTC 1 cut(s) 6272
AspA2I CCTAGG 1 cut(s) 26
AspLEI GCGC 3 cut(s) 22, 24, 1650
AsuC2I CCSGG 3 cut(s) 713, 4698, 4699
AsuNHI GCTAGC 1 cut(s) 4843
AvaI CYCGRG 4 cut(s) 415, 3159, 4697, 6715
AvaII GGWCC 6 cut(s) 1575, 2088, 2123, 4295, 5633, 6497
AvrII CCTAGG 1 cut(s) 26
BaeGI GKGCMC 3 cut(s) 2698, 3167, 6313
BaeI ACNNNNGTAYC 4 cut(s) 1772, 1772, 1805, 1805
BalI TGGCCA 1 cut(s) 2963
BanII GRGCYC 2 cut(s) 3167, 5988
BarI GAAGNNNNNNTAC 2 cut(s) 3633, 3665
BauI CACGAG 4 cut(s) 440, 1902, 4577, 6807
BbsI GAAGAC 4 cut(s) 4436, 5508, 5741, 6815
Bbv12I GWGCWC 6 cut(s) 2698, 2816, 3590, 4218, 4796, 6313
BbvCI CCTCAGC 2 cut(s) 690, 3633
BceAI ACGGC 3 cut(s) 356, 688, 6672
BciVI GTATCC 1 cut(s) 928
BclI TGATCA 1 cut(s) 6769
BcnI CCSGG 3 cut(s) 713, 4698, 4699
BcoDI GTCTC 6 cut(s) 74, 1765, 3014, 4574, 5367, 6718
BcuI ACTAGT 2 cut(s) 3521, 4825
BfmI CTRYAG 2 cut(s) 5258, 6817
BfrI CTTAAG 1 cut(s) 500
BfuAI ACCTGC 1 cut(s) 1106
BfuI GTATCC 1 cut(s) 928
BglII AGATCT 1 cut(s) 3808
BlnI CCTAGG 1 cut(s) 26
BmcAI AGTACT 2 cut(s) 4731, 6139
Bme18I GGWCC 6 cut(s) 1575, 2088, 2123, 4295, 5633, 6497
BmeRI GACNNNNNGTC 1 cut(s) 2343
BmeT110I CYCGRG 4 cut(s) 415, 3159, 4697, 6715
BmgBI CACGTC 1 cut(s) 2371
BmiI GGNNCC 9 cut(s) 710, 1577, 3164, 3165, 3249, 5455, 5773, 6499, 6686
BmrI ACTGGG 1 cut(s) 1573
BmtI GCTAGC 1 cut(s) 4847
BmuI ACTGGG 1 cut(s) 1573
BpiI GAAGAC 4 cut(s) 4436, 5508, 5741, 6815
BplI GAGNNNNNCTC 6 cut(s) 3326, 3358, 4216, 4248, 6790, 6822
BpmI CTGGAG 3 cut(s) 152, 5463, 6122
Bpu10I CCTNAGC 3 cut(s) 690, 3633, 4212
BpuEI CTTGAG 2 cut(s) 3602, 4912
BpuMI CCSGG 3 cut(s) 713, 4698, 4699
BsaAI YACGTR 2 cut(s) 2693, 3371
BsaBI GATNNNNATC 3 cut(s) 1125, 3873, 4246
BsaHI GRCGYC 1 cut(s) 1946
BsaI GGTCTC 1 cut(s) 74
BsaWI WCCGGW 3 cut(s) 1283, 1791, 4183
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 2798, 2828, 5255, 5285
Bsc4I CCNNNNNNNGG 6 cut(s) 1540, 1725, 1798, 2970, 6080, 6785
Bse118I RCCGGY 4 cut(s) 119, 345, 4183, 5195
Bse1I ACTGG 5 cut(s) 1177, 1579, 1719, 1847, 6848
Bse3DI GCAATG 6 cut(s) 1333, 2091, 2575, 3951, 4408, 4445
Bse8I GATNNNNATC 3 cut(s) 1125, 3873, 4246
BseJI GATNNNNATC 3 cut(s) 1125, 3873, 4246
BseLI CCNNNNNNNGG 6 cut(s) 1540, 1725, 1798, 2970, 6080, 6785
BseMI GCAATG 6 cut(s) 1333, 2091, 2575, 3951, 4408, 4445
BseNI ACTGG 5 cut(s) 1177, 1579, 1719, 1847, 6848
BsePI GCGCGC 1 cut(s) 20
BseRI GAGGAG 6 cut(s) 92, 212, 707, 5368, 5516, 5808
BseSI GKGCMC 3 cut(s) 2698, 3167, 6313
BsgI GTGCAG 1 cut(s) 6035
Bsh1236I CGCG 3 cut(s) 22, 4863, 6797
Bsh1285I CGRYCG 1 cut(s) 4188
BshTI ACCGGT 1 cut(s) 4183
BsiEI CGRYCG 1 cut(s) 4188
BsiHKAI GWGCWC 6 cut(s) 2698, 2816, 3590, 4218, 4796, 6313
BsiHKCI CYCGRG 4 cut(s) 415, 3159, 4697, 6715
BslFI GGGAC 6 cut(s) 1277, 1388, 1561, 2222, 2927, 6483
BslI CCNNNNNNNGG 6 cut(s) 1540, 1725, 1798, 2970, 6080, 6785
BsmAI GTCTC 6 cut(s) 74, 1765, 3014, 4574, 5367, 6718
BsmFI GGGAC 6 cut(s) 1277, 1388, 1561, 2222, 2927, 6483
BsmI GAATGC 3 cut(s) 3465, 3568, 6650
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 4 cut(s) 415, 3159, 4697, 6715
Bsp120I GGGCCC 1 cut(s) 3163
Bsp1286I GDGCHC 8 cut(s) 2698, 2816, 3167, 3590, 4218, 4796, 5988, 6313
Bsp1407I TGTACA 4 cut(s) 1627, 1767, 1930, 3939
Bsp19I CCATGG 4 cut(s) 72, 3167, 3557, 5447
Bsp68I TCGCGA 1 cut(s) 6797
BspFNI CGCG 3 cut(s) 22, 4863, 6797
BspHI TCATGA 1 cut(s) 3469
BspLI GGNNCC 9 cut(s) 710, 1577, 3164, 3165, 3249, 5455, 5773, 6499, 6686
BspMAI CTGCAG 1 cut(s) 6821
BspMI ACCTGC 1 cut(s) 1106
BspOI GCTAGC 1 cut(s) 4847
BspTI CTTAAG 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 74
BsrBI CCGCTC 3 cut(s) 264, 673, 1456
BsrDI GCAATG 6 cut(s) 1333, 2091, 2575, 3951, 4408, 4445
BsrFI RCCGGY 4 cut(s) 119, 345, 4183, 5195
BsrGI TGTACA 4 cut(s) 1627, 1767, 1930, 3939
BsrI ACTGG 5 cut(s) 1177, 1579, 1719, 1847, 6848
BssAI RCCGGY 4 cut(s) 119, 345, 4183, 5195
BssHII GCGCGC 1 cut(s) 20
BssNI GRCGYC 1 cut(s) 1946
BssSI CACGAG 4 cut(s) 440, 1902, 4577, 6807
BssT1I CCWWGG 7 cut(s) 26, 72, 3167, 3557, 5447, 5636, 6026
Bst2BI CACGAG 4 cut(s) 440, 1902, 4577, 6807
Bst6I CTCTTC 7 cut(s) 338, 1682, 3409, 3597, 3999, 4754, 5318
BstACI GRCGYC 1 cut(s) 1946
BstAFI CTTAAG 1 cut(s) 500
BstAPI GCANNNNNTGC 2 cut(s) 3825, 6317
BstAUI TGTACA 4 cut(s) 1627, 1767, 1930, 3939
BstBAI YACGTR 2 cut(s) 2693, 3371
BstDSI CCRYGG 6 cut(s) 72, 704, 3167, 3557, 5447, 6373
BstFNI CGCG 3 cut(s) 22, 4863, 6797
BstHHI GCGC 3 cut(s) 22, 24, 1650
BstMAI GTCTC 6 cut(s) 74, 1765, 3014, 4574, 5367, 6718
BstMCI CGRYCG 1 cut(s) 4188
BstNSI RCATGY 6 cut(s) 735, 3929, 5096, 6073, 6135, 6167
BstSFI CTRYAG 2 cut(s) 5258, 6817
BstSLI GKGCMC 3 cut(s) 2698, 3167, 6313
BstUI CGCG 3 cut(s) 22, 4863, 6797
BstV2I GAAGAC 4 cut(s) 4436, 5508, 5741, 6815
BstX2I RGATCY 4 cut(s) 817, 1094, 3808, 4692
BstXI CCANNNNNNTGG 3 cut(s) 1133, 2508, 6380
BstYI RGATCY 4 cut(s) 817, 1094, 3808, 4692
BsuI GTATCC 1 cut(s) 928
BtgI CCRYGG 6 cut(s) 72, 704, 3167, 3557, 5447, 6373
BtrI CACGTC 1 cut(s) 2371
BtsI GCAGTG 2 cut(s) 2150, 5952
BtuMI TCGCGA 1 cut(s) 6797
BveI ACCTGC 1 cut(s) 1106
CaiI CAGNNNCTG 3 cut(s) 4211, 5266, 6418
CciI TCATGA 1 cut(s) 3469
CfoI GCGC 3 cut(s) 22, 24, 1650
Cfr10I RCCGGY 4 cut(s) 119, 345, 4183, 5195
Cfr9I CCCGGG 1 cut(s) 4697
CseI GACGC 2 cut(s) 628, 1935
CspAI ACCGGT 1 cut(s) 4183
CspCI CAANNNNNGTGG 2 cut(s) 5446, 5481
DraI TTTAAA 5 cut(s) 1104, 2929, 4045, 4374, 4494
DrdI GACNNNNNNGTC 1 cut(s) 980
DriI GACNNNNNGTC 1 cut(s) 2343
DseDI GACNNNNNNGTC 1 cut(s) 980
EaeI YGGCCR 4 cut(s) 366, 636, 2961, 6370
Eam1104I CTCTTC 7 cut(s) 338, 1682, 3409, 3597, 3999, 4754, 5318
Eam1105I GACNNNNNGTC 1 cut(s) 2343
EarI CTCTTC 7 cut(s) 338, 1682, 3409, 3597, 3999, 4754, 5318
EciI GGCGGA 2 cut(s) 106, 3773
Eco130I CCWWGG 7 cut(s) 26, 72, 3167, 3557, 5447, 5636, 6026
Eco24I GRGCYC 2 cut(s) 3167, 5988
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 4 cut(s) 2746, 3199, 3284, 4244
Eco47I GGWCC 6 cut(s) 1575, 2088, 2123, 4295, 5633, 6497
Eco57I CTGAAG 9 cut(s) 134, 1020, 1545, 2597, 3578, 4107, 5436, 6342, 6753
Eco88I CYCGRG 4 cut(s) 415, 3159, 4697, 6715
EcoO109I RGGNCCY 3 cut(s) 2123, 3163, 4126
EcoRV GATATC 4 cut(s) 2746, 3199, 3284, 4244
EcoT14I CCWWGG 7 cut(s) 26, 72, 3167, 3557, 5447, 5636, 6026
EcoT22I ATGCAT 5 cut(s) 1435, 3480, 5296, 6214, 6405
EcoT38I GRGCYC 2 cut(s) 3167, 5988
ErhI CCWWGG 7 cut(s) 26, 72, 3167, 3557, 5447, 5636, 6026
FalI AAGNNNNNCTT 4 cut(s) 3495, 3527, 4992, 5024
FaqI GGGAC 6 cut(s) 1277, 1388, 1561, 2222, 2927, 6483
FauI CCCGC 3 cut(s) 666, 4092, 6508
FauNDI CATATG 4 cut(s) 1852, 3346, 3480, 5803
FbaI TGATCA 1 cut(s) 6769
FblI GTMKAC 2 cut(s) 2164, 2373
FriOI GRGCYC 2 cut(s) 3167, 5988
FspI TGCGCA 1 cut(s) 1649
GlaI GCGC 3 cut(s) 21, 23, 1649
GsuI CTGGAG 3 cut(s) 152, 5463, 6122
HgaI GACGC 2 cut(s) 628, 1935
HhaI GCGC 3 cut(s) 22, 24, 1650
Hin1I GRCGYC 1 cut(s) 1946
Hin6I GCGC 3 cut(s) 20, 22, 1648
HinP1I GCGC 3 cut(s) 20, 22, 1648
HincII GTYRAC 3 cut(s) 3456, 5330, 5467
HindII GTYRAC 3 cut(s) 3456, 5330, 5467
HindIII AAGCTT 7 cut(s) 2909, 3181, 3663, 3725, 5001, 5579, 6735
HpaI GTTAAC 1 cut(s) 3456
Hpy99I CGWCG 3 cut(s) 140, 4868, 5592
HpyCH4IV ACGT 8 cut(s) 1912, 2025, 2167, 2370, 2692, 3370, 4050, 4866
HpySE526I ACGT 8 cut(s) 1912, 2025, 2167, 2370, 2692, 3370, 4050, 4866
Hsp92I GRCGYC 1 cut(s) 1946
HspAI GCGC 3 cut(s) 20, 22, 1648
KroI GCCGGC 2 cut(s) 119, 5195
KroNI GCCGGC 2 cut(s) 121, 5197
Ksp22I TGATCA 1 cut(s) 6769
KspAI GTTAAC 1 cut(s) 3456
MaeII ACGT 8 cut(s) 1912, 2025, 2167, 2370, 2692, 3370, 4050, 4866
MbiI CCGCTC 3 cut(s) 264, 673, 1456
MfeI CAATTG 3 cut(s) 1355, 3204, 3699
MflI RGATCY 4 cut(s) 817, 1094, 3808, 4692
MhlI GDGCHC 8 cut(s) 2698, 2816, 3167, 3590, 4218, 4796, 5988, 6313
MlsI TGGCCA 1 cut(s) 2963
MluNI TGGCCA 1 cut(s) 2963
Mox20I TGGCCA 1 cut(s) 2963
Mph1103I ATGCAT 5 cut(s) 1435, 3480, 5296, 6214, 6405
MroNI GCCGGC 2 cut(s) 119, 5195
MroXI GAANNNNTTC 1 cut(s) 6272
MscI TGGCCA 1 cut(s) 2963
MslI CAYNNNNRTG 8 cut(s) 1554, 1755, 3051, 3571, 5283, 5996, 6378, 6850
Msp20I TGGCCA 1 cut(s) 2963
MspA1I CMGCKG 2 cut(s) 4099, 5680
MspCI CTTAAG 1 cut(s) 500
MunI CAATTG 3 cut(s) 1355, 3204, 3699
Mva1269I GAATGC 3 cut(s) 3465, 3568, 6650
MvnI CGCG 3 cut(s) 22, 4863, 6797
NaeI GCCGGC 2 cut(s) 121, 5197
NciI CCSGG 3 cut(s) 713, 4698, 4699
NcoI CCATGG 4 cut(s) 72, 3167, 3557, 5447
NdeI CATATG 4 cut(s) 1852, 3346, 3480, 5803
NgoMIV GCCGGC 2 cut(s) 119, 5195
NheI GCTAGC 1 cut(s) 4843
NlaIV GGNNCC 9 cut(s) 710, 1577, 3164, 3165, 3249, 5455, 5773, 6499, 6686
NmuCI GTSAC 5 cut(s) 616, 2494, 3530, 4474, 6556
NruI TCGCGA 1 cut(s) 6797
NsbI TGCGCA 1 cut(s) 1649
NsiI ATGCAT 5 cut(s) 1435, 3480, 5296, 6214, 6405
NspI RCATGY 6 cut(s) 735, 3929, 5096, 6073, 6135, 6167
OliI CACNNNNGTG 1 cut(s) 6378
PaeI GCATGC 3 cut(s) 3929, 6073, 6167
PaeR7I CTCGAG 1 cut(s) 6715
PagI TCATGA 1 cut(s) 3469
PalAI GGCGCGCC 1 cut(s) 20
PaqCI CACCTGC 1 cut(s) 1106
PauI GCGCGC 1 cut(s) 20
PciI ACATGT 2 cut(s) 731, 5092
PcsI WCGNNNNNNNCGW 2 cut(s) 1909, 6200
PctI GAATGC 3 cut(s) 3465, 3568, 6650
PdiI GCCGGC 2 cut(s) 121, 5197
PdmI GAANNNNTTC 1 cut(s) 6272
PflFI GACNNNGTC 1 cut(s) 3262
PflMI CCANNNNNTGG 2 cut(s) 1725, 2970
PinAI ACCGGT 1 cut(s) 4183
Ppu21I YACGTR 2 cut(s) 2693, 3371
PpuMI RGGWCCY 1 cut(s) 2123
PscI ACATGT 2 cut(s) 731, 5092
PshBI ATTAAT 1 cut(s) 2760
PsiI TTATAA 1 cut(s) 2229
Psp5II RGGWCCY 1 cut(s) 2123
PspN4I GGNNCC 9 cut(s) 710, 1577, 3164, 3165, 3249, 5455, 5773, 6499, 6686
PspOMI GGGCCC 1 cut(s) 3163
PspPPI RGGWCCY 1 cut(s) 2123
PsrI GAACNNNNNNTAC 4 cut(s) 892, 924, 6445, 6477
PstI CTGCAG 1 cut(s) 6821
PstNI CAGNNNCTG 3 cut(s) 4211, 5266, 6418
PsuI RGATCY 4 cut(s) 817, 1094, 3808, 4692
PsyI GACNNNGTC 1 cut(s) 3262
PteI GCGCGC 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 5680
RruI TCGCGA 1 cut(s) 6797
RseI CAYNNNNRTG 8 cut(s) 1554, 1755, 3051, 3571, 5283, 5996, 6378, 6850
ScaI AGTACT 2 cut(s) 4731, 6139
SduI GDGCHC 8 cut(s) 2698, 2816, 3167, 3590, 4218, 4796, 5988, 6313
SfcI CTRYAG 2 cut(s) 5258, 6817
Sfr274I CTCGAG 1 cut(s) 6715
SgsI GGCGCGCC 1 cut(s) 20
SinI GGWCC 6 cut(s) 1575, 2088, 2123, 4295, 5633, 6497
SlaI CTCGAG 1 cut(s) 6715
SmaI CCCGGG 1 cut(s) 4699
SmiI ATTTAAAT 2 cut(s) 1104, 2929
SmiMI CAYNNNNRTG 8 cut(s) 1554, 1755, 3051, 3571, 5283, 5996, 6378, 6850
SmlI CTYRAG 4 cut(s) 500, 3581, 4927, 6715
SmoI CTYRAG 4 cut(s) 500, 3581, 4927, 6715
SpeI ACTAGT 2 cut(s) 3521, 4825
SphI GCATGC 3 cut(s) 3929, 6073, 6167
SspI AATATT 2 cut(s) 1834, 3788
StyI CCWWGG 7 cut(s) 26, 72, 3167, 3557, 5447, 5636, 6026
SwaI ATTTAAAT 2 cut(s) 1104, 2929
TaiI ACGT 8 cut(s) 1915, 2028, 2170, 2373, 2695, 3373, 4053, 4869
TaqII GACCGA 2 cut(s) 866, 4224
TauI GCSGC 5 cut(s) 143, 371, 638, 1456, 4863
TseFI GTSAC 5 cut(s) 616, 2494, 3530, 4474, 6556
Tsp45I GTSAC 5 cut(s) 616, 2494, 3530, 4474, 6556
TspGWI ACGGA 3 cut(s) 4958, 6192, 6390
TspMI CCCGGG 1 cut(s) 4697
Tth111I GACNNNGTC 1 cut(s) 3262
Van91I CCANNNNNTGG 2 cut(s) 1725, 2970
Vha464I CTTAAG 1 cut(s) 500
VneI GTGCAC 2 cut(s) 2694, 6309
VpaK11BI GGWCC 6 cut(s) 1575, 2088, 2123, 4295, 5633, 6497
VspI ATTAAT 1 cut(s) 2760
XbaI TCTAGA 1 cut(s) 4238
XceI RCATGY 6 cut(s) 735, 3929, 5096, 6073, 6135, 6167
XcmI CCANNNNNNNNNTGG 1 cut(s) 4268
XhoI CTCGAG 1 cut(s) 6715
XmaI CCCGGG 1 cut(s) 4697
XmaJI CCTAGG 1 cut(s) 26
XmiI GTMKAC 2 cut(s) 2164, 2373
XmnI GAANNNNTTC 1 cut(s) 6272
ZrmI AGTACT 2 cut(s) 4731, 6139
Zsp2I ATGCAT 5 cut(s) 1435, 3480, 5296, 6214, 6405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.