pycom17g24700

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000856
Physical Location & Seq
Forward (+)
22916112 .. 22919192
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2997 bp
ATGGTGTTCGTCACATCACCAAATCACAAAACCCTAGCCCTAAATCTAAACCCTAAGATCACCACCCTCCAAACCCTAAAGCTCCAAATCGAGCAAAATTCTCACATACCCATTTCTCAGCAGCGCCTATTCATCTCCCAAAGCCTCCAGTTACTCACCCAATCCGACTCCGCCCTCCTCTCCGACCTTGGAATCCGACCCCTTTCAACCCTAACTCTTCACATTCCCTTATTCGGCGGCACTCAACCTCCCGCCGTCCCTAAACCCCGACTCGAGTTCCTCAACTCCAAGCCTCCCCCGAATTATGTCGCCGGGCTCGGCCGTGGTGCCACTGGCTTCACCACCAGGTCCGATATCGGGCCCGCACGTGCTGCGCCCGACCTGCCGGATAGGTCGGCCACGACAATCGATAAGGGGTACGATGAGAATCAGAAGTTTGATGAATTCGAAGGAAATGATGTCGGGTTGTTTGCATCGGCGGAATACGATGATGAGGACAAGGAGGCTGATGCGGTGTGGGAAGCCATTGAGCAAAGAATGGACTCGAGGAGGAAGGACAGGAGAGAAGCTCGGTTGAAAGAAGAGATTGAAAAGTACAGAGCTTCGAACCCGAAGATCACGGAGCAATTCGCGACTTTGAAGAGGAAGTTATACACTGTGTCTGCTCAGGAATGGGAGAGCATACCTGAAATCGGAGACTATTCGTCGAGGAACAAGAAGAAGAGATTCGAGAGCTTTGTGCCGGTGCCCGATACACTTTTAGAGAAGGCAAGACAGGAAAAGGAGCATGTTACGGCATTGGACCCCAAGAGTAGGGGAGCTGGTGGTATGGAGACTCCATGGTCTCAAACTCCGGTTACGGATTTGACTGCTGTAGGTGAGGGTAGAGGTACCGTTTTGTCATTGAAATTGGATAGGCTTTCTGATTCTGTTTCTGGATTGACGGTTGTGGACCCGAAAGGGTACCTTACTGATCTTAAGAGTATGAAGATTACTAGTGATGCCGAGATTTCAGATATTAAAAAGGCTAGGTTGTTGCTTAAGAGTGTTACACAGACGAATCCGAAGCACCCACCTGGATGGATTGCGGCTGCCAGGCTGGAGGAGGTGGCTGGGAAGATTCAGGCAGCTAGACAATTGATTCAGAAAGGGTGTGAGGAATGTCCTAAGAGTGAGGATGTGTGGTTAGAGGCGTGTAGGCTTTCAAGCCCTGATGAAGCTAAGGCCGTGATTTCTAAGGGAGTGAAGTCAATTCCCAATTCGGTGAAGTTGTGGATGCAGGCTGCGAAATTGGAACATGATGATTTGAATAGGAGCAGGGTATTGAGGAAAGGTTTGGAACACATTCCCGATTCTGTTAGGTTATGGAAGGCGGTGGTGGAGCTTGCCAATGAGGAGGATGCGAGACTTTTGCTTCATAGGGCTGTGGAGTGTTGTCCCTTGCACATTGAGTTATGGCTTGCACTTGCAAGATTGGAAACTTATGAGAATGCGAGGAAGGTCCTTAATAGGGCAAGAGAGAAGCTATCAAAAGAGCCTGCCATTTGGATCACAGCAGCGAAGTTGGAGGAAGCTAATGGGAACACAGCCATGGTTGGGAAGATTATTGAAAGGGGTATAAGGGCTTTGCAAAGAGAAGGGTTGGCGATTGATAGAGAGGCATGGATGAAGGAGGCTGAAGCTGCTGAACGTGCAGGGTCTGTTGCAACTTGCCAAGCAATCATTTGGAACACGATTGGGATTGGTGTGGAGGAAGAGGATAGGAAGAGGACATGGGTTGCTGATGCTGAGGAATGCAAGAAAAGGGGTTCCATTGAAACAGCCAGAGCTATTTATGCACACGCACTTACTGTCTTTTTAACCAAGAAGAGCATATGGCTCAAAGCCGCACAGCTTGAAAAGAGCCATGGTACTAGGGAATCTCTTGATGCTTTACTTCGTAAAGCGGTGACATACCGACCACAGGCTGAAGTCTTGTGGCTTATGGGAGCTAAGGAGAAGTGGCTTGCTGGGGATGTCCCTGCTGCTCGTGCCATCCTTCAAGAAGCTTATGCTGCCATTCCTAATTCTGAAGAAATTTGGCTTGCTGCGTTTAAACTTGAATTTGAGAACCATGAACCAGAGAGAGCCAGAATGCTACTTGCCAAAGCCCGAGAAAAGGGAGGTACTGAAAGAGTGTGGATGAAATCAGCAATTGTTGAGAGAGAATTAGGGAACATTAATGAGGAGAGGAAGTTGATTGATGATGGGTTGAAGCGCTTCCCTTCCTTCTATAAATTATGGTTGATGCTTGGACAGCTAGAGGAACGCCTTGGTCATTTGGAAAAGGCCAAGGAGGCCTATGATTCAGGTCAGAAGCACTGCTCCAGTTCTATTCCACTGTGGCTTTCTCTTGCTAATCTTGAAGAGAAGATGAGTGGGCTGAGTAAAGCTCGTGCAATTCTAACAATGGCCAGGAAGAAGAATCCTCAGAACCCTGAATTGTGGCTTGCTGCTGTTCGAGCTGAATTAAGGCATGGAAATAAGAAGGAAGCTGATATCTTGATGGCAAAGGCTTTGCAGGAGTGTCCCAATAGTGGTATCTTGTGGGCTGCATCTATTGAGATGGTACCCCGTCCGCAACGGAAGACGAAGAGTATGGATGCCCTCAAGAAATGCGATCATGATCCCCATGTCATTGCTGCTGTGTCCAAGTTATTTTGGCATGACAGGAAAGTTGACAAAGCTAGGACTTGGCTTAACAGGGCAGTGACACTAGCGCCAGACATTGGGGATTTCTGGGCTTTATACTACAAATTTGAACTTCAACATGGGACCGAGGAGAACCAGAAAGATGTACTTAAAAGATGCATAGCTGCAGAACCAAAGCATGGTGAAAAATGGCAACCTATTTCAAAGGCCGTGGAGAACTCCCACCAACCAACTGAAGCTGTCTTGAAGAAAGTGGTCGTTGCTCTTGGGAAGGAAGAGAGTGCAGCTGAAAATAATAAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000387 GO:0000398 GO:0003674 GO:0003676 GO:0003712 GO:0003713 GO:0003723 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005684 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006403 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009628 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0015030 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0031047 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0034622 GO:0034641 GO:0035257 GO:0035258 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046540 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051252 GO:0051254 GO:0051427 GO:0060147 GO:0060148 GO:0060255 GO:0060964 GO:0060966 GO:0060968 GO:0065003 GO:0065007 GO:0070013 GO:0070920 GO:0071005 GO:0071011 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090351 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140110 GO:1901360 GO:1901363 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1903798 GO:1903800 GO:1990904 GO:2000112 GO:2000628 GO:2000630 GO:2000634 GO:2000636 GO:2000637 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
PRP1_N TPR_14 TPR_16 TPR_19 TPR_8 ubiquitin

Protein Analysis

999

Amino Acids

112.12

Weight (kDa)

8.78

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP1_N PF06424 98 - 237 7.1e-52 PRP1 splicing factor, N-terminal
Suf PF05843 393 - 546 5.4e-07 Suppressor of forked protein (Suf)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 841
Acc36I ACCTGC 1 cut(s) 390
Acc65I GGTACC 3 cut(s) 890, 963, 2608
AccB1I GGYRCC 5 cut(s) 326, 745, 890, 963, 2608
AccB7I CCANNNNNTGG 2 cut(s) 2768, 2870
AccII CGCG 1 cut(s) 632
AclWI GGATC 2 cut(s) 1556, 2660
AcoI YGGCCR 3 cut(s) 319, 396, 2451
AcsI RAATTY 5 cut(s) 97, 443, 2076, 2102, 2795
AcuI CTGAAG 4 cut(s) 1698, 1989, 2091, 2946
AcvI CACGTG 1 cut(s) 368
AfaI GTAC 8 cut(s) 419, 596, 892, 965, 1912, 2167, 2610, 2838
AfeI AGCGCT 1 cut(s) 2258
AflII CTTAAG 2 cut(s) 977, 1040
AhdI GACNNNNNGTC 1 cut(s) 703
AhlI ACTAGT 1 cut(s) 995
AjnI CCWGG 4 cut(s) 344, 1075, 1094, 2453
Alw26I GTCTC 4 cut(s) 690, 827, 849, 1399
AlwI GGATC 2 cut(s) 1556, 2660
AlwNI CAGNNNCTG 1 cut(s) 1700
Ama87I CYCGRG 3 cut(s) 272, 544, 2151
Aor51HI AGCGCT 1 cut(s) 2258
AoxI GGCC 8 cut(s) 319, 359, 396, 1224, 2328, 2337, 2451, 2898
ApaI GGGCCC 1 cut(s) 363
ApoI RAATTY 5 cut(s) 97, 443, 2076, 2102, 2795
AseI ATTAAT 1 cut(s) 2220
Asp700I GAANNNNTTC 3 cut(s) 725, 1344, 2258
Asp718I GGTACC 3 cut(s) 890, 963, 2608
AspLEI GCGC 4 cut(s) 126, 376, 2259, 2761
AspS9I GGNCC 7 cut(s) 348, 359, 360, 802, 952, 1501, 2814
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 8 cut(s) 9, 52, 148, 331, 890, 1276, 1960, 2885
AsuII TTCGAA 2 cut(s) 447, 605
AvaI CYCGRG 3 cut(s) 272, 544, 2151
AvaII GGWCC 5 cut(s) 348, 802, 952, 1501, 2814
BaeGI GKGCMC 2 cut(s) 363, 750
BalI TGGCCA 1 cut(s) 2453
BanI GGYRCC 5 cut(s) 326, 745, 890, 963, 2608
BanII GRGCYC 2 cut(s) 318, 363
BauI CACGAG 2 cut(s) 2028, 2433
BbrPI CACGTG 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 2633
BbvCI CCTCAGC 1 cut(s) 1788
BccI CCATC 5 cut(s) 1074, 2042, 2240, 2539, 2599
BceAI ACGGC 5 cut(s) 239, 306, 810, 1211, 2885
BcgI CGANNNNNNTGC 2 cut(s) 1393, 1427
BciT130I CCWGG 4 cut(s) 346, 1077, 1096, 2455
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 4 cut(s) 690, 827, 849, 1399
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 8 cut(s) 35, 996, 1029, 1131, 1914, 2300, 2727, 2756
BfmI CTRYAG 2 cut(s) 873, 2856
BfoI RGCGCY 3 cut(s) 127, 2260, 2762
BfrI CTTAAG 2 cut(s) 977, 1040
BfuAI ACCTGC 1 cut(s) 390
BglI GCCNNNNNGGC 1 cut(s) 2336
Bme1390I CCNGG 5 cut(s) 313, 346, 1077, 1096, 2455
Bme18I GGWCC 5 cut(s) 348, 802, 952, 1501, 2814
BmeRI GACNNNNNGTC 1 cut(s) 703
BmeT110I CYCGRG 3 cut(s) 272, 544, 2151
BmgT120I GGNCC 7 cut(s) 348, 359, 360, 802, 952, 1501, 2814
BmrFI CCNGG 5 cut(s) 313, 346, 1077, 1096, 2455
BpiI GAAGAC 1 cut(s) 2633
BplI GAGNNNNNCTC 4 cut(s) 553, 585, 2416, 2448
BpmI CTGGAG 3 cut(s) 131, 1121, 2350
Bpu10I CCTNAGC 4 cut(s) 666, 1221, 1788, 1992
Bpu14I TTCGAA 2 cut(s) 447, 605
BpuEI CTTGAG 1 cut(s) 2633
BpuMI CCSGG 1 cut(s) 313
Bsa29I ATCGAT 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 368
BsaBI GATNNNNATC 2 cut(s) 426, 2664
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 9 cut(s) 187, 322, 839, 1590, 1906, 2311, 2331, 2817, 2901
BsaWI WCCGGW 1 cut(s) 853
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 2219, 2249
Bse118I RCCGGY 1 cut(s) 742
Bse1I ACTGG 3 cut(s) 148, 337, 2367
Bse3DI GCAATG 1 cut(s) 2676
Bse8I GATNNNNATC 2 cut(s) 426, 2664
BseBI CCWGG 4 cut(s) 346, 1077, 1096, 2455
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 9 cut(s) 187, 322, 839, 1590, 1906, 2311, 2331, 2817, 2901
BseGI GGATG 9 cut(s) 1085, 1183, 1281, 1405, 1671, 2020, 2034, 2187, 2647
BseJI GATNNNNATC 2 cut(s) 426, 2664
BseMI GCAATG 1 cut(s) 2676
BseMII CTCAG 5 cut(s) 131, 680, 1779, 2414, 2483
BseNI ACTGG 3 cut(s) 148, 337, 2367
BseRI GAGGAG 6 cut(s) 167, 562, 1118, 1409, 2240, 2834
BseSI GKGCMC 2 cut(s) 363, 750
BseX3I CGGCCG 1 cut(s) 319
BseYI CCCAGC 2 cut(s) 1112, 2009
BsgI GTGCAG 2 cut(s) 1713, 2994
Bsh1236I CGCG 1 cut(s) 632
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 8 cut(s) 321, 361, 398, 1226, 2330, 2339, 2453, 2900
BshNI GGYRCC 5 cut(s) 326, 745, 890, 963, 2608
BshVI ATCGAT 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 322
BsiHKCI CYCGRG 3 cut(s) 272, 544, 2151
BsiSI CCGG 4 cut(s) 312, 386, 743, 854
BslFI GGGAC 5 cut(s) 242, 1422, 2003, 2553, 2827
BsmAI GTCTC 4 cut(s) 690, 827, 849, 1399
BsmFI GGGAC 5 cut(s) 242, 1422, 2003, 2553, 2827
BsmI GAATGC 3 cut(s) 1495, 1799, 2139
BsnI GGCC 8 cut(s) 321, 361, 398, 1226, 2330, 2339, 2453, 2900
Bso31I GGTCTC 1 cut(s) 849
BsoBI CYCGRG 3 cut(s) 272, 544, 2151
Bsp119I TTCGAA 2 cut(s) 447, 605
Bsp120I GGGCCC 1 cut(s) 359
Bsp1286I GDGCHC 3 cut(s) 318, 363, 750
Bsp143I GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
Bsp19I CCATGG 3 cut(s) 839, 1590, 1906
Bsp68I TCGCGA 1 cut(s) 632
BspANI GGCC 8 cut(s) 321, 361, 398, 1226, 2330, 2339, 2453, 2900
BspCNI CTCAG 5 cut(s) 130, 679, 1780, 2415, 2482
BspDI ATCGAT 1 cut(s) 408
BspFNI CGCG 1 cut(s) 632
BspHI TCATGA 1 cut(s) 2662
BspMAI CTGCAG 1 cut(s) 2860
BspMI ACCTGC 1 cut(s) 390
BspPI GGATC 2 cut(s) 1556, 2660
BspQI GCTCTTC 1 cut(s) 1862
BspT104I TTCGAA 2 cut(s) 447, 605
BspT107I GGYRCC 5 cut(s) 326, 745, 890, 963, 2608
BspTI CTTAAG 2 cut(s) 977, 1040
BspTNI GGTCTC 1 cut(s) 849
BsrDI GCAATG 1 cut(s) 2676
BsrFI RCCGGY 1 cut(s) 742
BsrI ACTGG 3 cut(s) 148, 337, 2367
BssAI RCCGGY 1 cut(s) 742
BssECI CCNNGG 9 cut(s) 187, 322, 839, 1590, 1906, 2311, 2331, 2817, 2901
BssMI GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
BssSI CACGAG 2 cut(s) 2028, 2433
BssT1I CCWWGG 6 cut(s) 187, 839, 1590, 1906, 2311, 2331
Bst2BI CACGAG 2 cut(s) 2028, 2433
Bst2UI CCWGG 4 cut(s) 346, 1077, 1096, 2455
Bst4CI ACNGT 6 cut(s) 658, 895, 946, 1852, 2382, 2994
BstAFI CTTAAG 2 cut(s) 977, 1040
BstAPI GCANNNNNTGC 1 cut(s) 371
BstBAI YACGTR 1 cut(s) 368
BstBI TTCGAA 2 cut(s) 447, 605
BstC8I GCNNGC 8 cut(s) 363, 1281, 1386, 1461, 1539, 2007, 2085, 2490
BstDSI CCRYGG 5 cut(s) 322, 839, 1590, 1906, 2901
BstF5I GGATG 9 cut(s) 1085, 1183, 1281, 1405, 1671, 2020, 2034, 2187, 2647
BstFNI CGCG 1 cut(s) 632
BstH2I RGCGCY 3 cut(s) 127, 2260, 2762
BstHHI GCGC 4 cut(s) 126, 376, 2259, 2761
BstKTI GATC 6 cut(s) 60, 618, 976, 1551, 2662, 2668
BstMAI GTCTC 4 cut(s) 690, 827, 849, 1399
BstMBI GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
BstMCI CGRYCG 1 cut(s) 322
BstNI CCWGG 4 cut(s) 346, 1077, 1096, 2455
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 5 cut(s) 311, 344, 1075, 1094, 2453
BstSFI CTRYAG 2 cut(s) 873, 2856
BstSLI GKGCMC 2 cut(s) 363, 750
BstUI CGCG 1 cut(s) 632
BstV2I GAAGAC 1 cut(s) 2633
BstXI CCANNNNNNTGG 1 cut(s) 1080
BstZI CGGCCG 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 8 cut(s) 321, 361, 398, 1226, 2330, 2339, 2453, 2900
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 5 cut(s) 322, 839, 1590, 1906, 2901
BtsCI GGATG 9 cut(s) 1085, 1183, 1281, 1405, 1671, 2020, 2034, 2187, 2647
BtsI GCAGTG 2 cut(s) 2359, 2754
BtsIMutI CAGTG 5 cut(s) 330, 654, 2359, 2378, 2754
BtuMI TCGCGA 1 cut(s) 632
BveI ACCTGC 1 cut(s) 390
Cac8I GCNNGC 8 cut(s) 363, 1281, 1386, 1461, 1539, 2007, 2085, 2490
CaiI CAGNNNCTG 1 cut(s) 1700
CciI TCATGA 1 cut(s) 2662
CfoI GCGC 4 cut(s) 126, 376, 2259, 2761
Cfr10I RCCGGY 1 cut(s) 742
Cfr13I GGNCC 7 cut(s) 348, 359, 360, 802, 952, 1501, 2814
ClaI ATCGAT 1 cut(s) 408
CsiI ACCWGGT 1 cut(s) 344
Csp6I GTAC 8 cut(s) 418, 595, 891, 964, 1911, 2166, 2609, 2837
CspCI CAANNNNNGTGG 2 cut(s) 2883, 2918
CviQI GTAC 8 cut(s) 418, 595, 891, 964, 1911, 2166, 2609, 2837
DpnI GATC 6 cut(s) 59, 617, 975, 1550, 2661, 2667
DpnII GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
DraI TTTAAA 1 cut(s) 2095
DrdI GACNNNNNNGTC 1 cut(s) 841
DriI GACNNNNNGTC 1 cut(s) 703
DseDI GACNNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 3 cut(s) 319, 396, 2451
EagI CGGCCG 1 cut(s) 319
Eam1105I GACNNNNNGTC 1 cut(s) 703
EciI GGCGGA 2 cut(s) 160, 494
EclXI CGGCCG 1 cut(s) 319
Eco130I CCWWGG 6 cut(s) 187, 839, 1590, 1906, 2311, 2331
Eco147I AGGCCT 1 cut(s) 2339
Eco24I GRGCYC 2 cut(s) 318, 363
Eco31I GGTCTC 1 cut(s) 849
Eco32I GATATC 2 cut(s) 355, 2539
Eco47I GGWCC 5 cut(s) 348, 802, 952, 1501, 2814
Eco47III AGCGCT 1 cut(s) 2258
Eco52I CGGCCG 1 cut(s) 319
Eco57I CTGAAG 4 cut(s) 1698, 1989, 2091, 2946
Eco72I CACGTG 1 cut(s) 368
Eco88I CYCGRG 3 cut(s) 272, 544, 2151
EcoO109I RGGNCCY 1 cut(s) 1501
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 4 cut(s) 344, 1075, 1094, 2453
EcoRV GATATC 2 cut(s) 355, 2539
EcoT14I CCWWGG 6 cut(s) 187, 839, 1590, 1906, 2311, 2331
EcoT22I ATGCAT 1 cut(s) 2852
EcoT38I GRGCYC 2 cut(s) 318, 363
ErhI CCWWGG 6 cut(s) 187, 839, 1590, 1906, 2311, 2331
FalI AAGNNNNNCTT 4 cut(s) 951, 983, 2823, 2855
FaqI GGGAC 5 cut(s) 242, 1422, 2003, 2553, 2827
FauI CCCGC 2 cut(s) 259, 370
FauNDI CATATG 1 cut(s) 1874
FokI GGATG 9 cut(s) 1092, 1190, 1288, 1412, 1678, 2021, 2027, 2194, 2654
FriOI GRGCYC 2 cut(s) 318, 363
FspBI CTAG 8 cut(s) 35, 996, 1029, 1131, 1914, 2300, 2727, 2756
GlaI GCGC 4 cut(s) 125, 375, 2258, 2760
GsaI CCCAGC 2 cut(s) 1116, 2013
GsuI CTGGAG 3 cut(s) 131, 1121, 2350
HaeII RGCGCY 3 cut(s) 127, 2260, 2762
HaeIII GGCC 8 cut(s) 321, 361, 398, 1226, 2330, 2339, 2453, 2900
HapII CCGG 4 cut(s) 312, 386, 743, 854
HhaI GCGC 4 cut(s) 126, 376, 2259, 2761
Hin6I GCGC 4 cut(s) 124, 374, 2257, 2759
HinP1I GCGC 4 cut(s) 124, 374, 2257, 2759
HincII GTYRAC 1 cut(s) 2719
HindII GTYRAC 1 cut(s) 2719
HindIII AAGCTT 1 cut(s) 2046
HpaII CCGG 4 cut(s) 312, 386, 743, 854
HphI GGTGA 8 cut(s) 9, 52, 148, 331, 890, 1276, 1960, 2885
Hpy166II GTNNAC 2 cut(s) 952, 2719
Hpy8I GTNNAC 2 cut(s) 952, 2719
Hpy99I CGWCG 1 cut(s) 709
HpyCH4III ACNGT 6 cut(s) 658, 895, 946, 1852, 2382, 2994
HpyCH4IV ACGT 2 cut(s) 367, 1690
HpySE526I ACGT 2 cut(s) 367, 1690
HspAI GCGC 4 cut(s) 124, 374, 2257, 2759
KpnI GGTACC 3 cut(s) 894, 967, 2612
Kzo9I GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
LguI GCTCTTC 1 cut(s) 1862
LmnI GCTCC 8 cut(s) 87, 622, 784, 818, 1314, 1381, 1988, 2369
MabI ACCWGGT 1 cut(s) 344
MaeI CTAG 8 cut(s) 35, 996, 1029, 1131, 1914, 2300, 2727, 2756
MaeII ACGT 2 cut(s) 367, 1690
MaeIII GTNAC 7 cut(s) 10, 150, 790, 856, 1048, 1948, 2749
MalI GATC 6 cut(s) 59, 617, 975, 1550, 2661, 2667
MboI GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
MfeI CAATTG 2 cut(s) 1136, 2193
MhlI GDGCHC 3 cut(s) 318, 363, 750
MlsI TGGCCA 1 cut(s) 2453
MluNI TGGCCA 1 cut(s) 2453
MlyI GAGTC 4 cut(s) 161, 264, 536, 829
MmeI TCCRAC 4 cut(s) 189, 207, 220, 1545
Mox20I TGGCCA 1 cut(s) 2453
Mph1103I ATGCAT 1 cut(s) 2852
MroXI GAANNNNTTC 3 cut(s) 725, 1344, 2258
MscI TGGCCA 1 cut(s) 2453
MslI CAYNNNNRTG 2 cut(s) 1078, 1589
Msp20I TGGCCA 1 cut(s) 2453
MspA1I CMGCKG 1 cut(s) 2978
MspCI CTTAAG 2 cut(s) 977, 1040
MspI CCGG 4 cut(s) 312, 386, 743, 854
MspR9I CCNGG 5 cut(s) 313, 346, 1077, 1096, 2455
MssI GTTTAAAC 1 cut(s) 2095
MunI CAATTG 2 cut(s) 1136, 2193
Mva1269I GAATGC 3 cut(s) 1495, 1799, 2139
MvaI CCWGG 4 cut(s) 346, 1077, 1096, 2455
MvnI CGCG 1 cut(s) 632
NciI CCSGG 1 cut(s) 313
NcoI CCATGG 3 cut(s) 839, 1590, 1906
NdeI CATATG 1 cut(s) 1874
NdeII GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
NmeAIII GCCGAG 2 cut(s) 297, 1030
NmuCI GTSAC 3 cut(s) 10, 1948, 2749
NruI TCGCGA 1 cut(s) 632
NsiI ATGCAT 1 cut(s) 2852
NspI RCATGY 1 cut(s) 791
NspV TTCGAA 2 cut(s) 447, 605
PaeR7I CTCGAG 2 cut(s) 272, 544
PagI TCATGA 1 cut(s) 2662
PceI AGGCCT 1 cut(s) 2339
PciSI GCTCTTC 1 cut(s) 1862
PctI GAATGC 3 cut(s) 1495, 1799, 2139
PdmI GAANNNNTTC 3 cut(s) 725, 1344, 2258
PflMI CCANNNNNTGG 2 cut(s) 2768, 2870
PleI GAGTC 4 cut(s) 161, 264, 536, 829
PmaCI CACGTG 1 cut(s) 368
PmeI GTTTAAAC 1 cut(s) 2095
PmlI CACGTG 1 cut(s) 368
PpsI GAGTC 4 cut(s) 161, 264, 536, 829
Ppu21I YACGTR 1 cut(s) 368
PpuMI RGGWCCY 1 cut(s) 1501
PshBI ATTAAT 1 cut(s) 2220
Psp5II RGGWCCY 1 cut(s) 1501
Psp6I CCWGG 4 cut(s) 344, 1075, 1094, 2453
PspCI CACGTG 1 cut(s) 368
PspFI CCCAGC 2 cut(s) 1112, 2009
PspGI CCWGG 4 cut(s) 344, 1075, 1094, 2453
PspOMI GGGCCC 1 cut(s) 359
PspPI GGNCC 7 cut(s) 348, 359, 360, 802, 952, 1501, 2814
PspPPI RGGWCCY 1 cut(s) 1501
PspXI VCTCGAGB 2 cut(s) 272, 544
PstI CTGCAG 1 cut(s) 2860
PstNI CAGNNNCTG 1 cut(s) 1700
PvuII CAGCTG 1 cut(s) 2978
RruI TCGCGA 1 cut(s) 632
RsaI GTAC 8 cut(s) 419, 596, 892, 965, 1912, 2167, 2610, 2838
RsaNI GTAC 8 cut(s) 418, 595, 891, 964, 1911, 2166, 2609, 2837
RseI CAYNNNNRTG 2 cut(s) 1078, 1589
SapI GCTCTTC 1 cut(s) 1862
Sau3AI GATC 6 cut(s) 57, 615, 973, 1548, 2659, 2665
Sau96I GGNCC 7 cut(s) 348, 359, 360, 802, 952, 1501, 2814
SchI GAGTC 4 cut(s) 161, 264, 536, 829
ScrFI CCNGG 5 cut(s) 313, 346, 1077, 1096, 2455
SduI GDGCHC 3 cut(s) 318, 363, 750
SexAI ACCWGGT 1 cut(s) 344
SfcI CTRYAG 2 cut(s) 873, 2856
SfiI GGCCNNNNNGGCC 1 cut(s) 2336
Sfr274I CTCGAG 2 cut(s) 272, 544
SfuI TTCGAA 2 cut(s) 447, 605
SinI GGWCC 5 cut(s) 348, 802, 952, 1501, 2814
SlaI CTCGAG 2 cut(s) 272, 544
SmiMI CAYNNNNRTG 2 cut(s) 1078, 1589
SmlI CTYRAG 5 cut(s) 272, 544, 977, 1040, 2648
SmoI CTYRAG 5 cut(s) 272, 544, 977, 1040, 2648
SpeI ACTAGT 1 cut(s) 995
SseBI AGGCCT 1 cut(s) 2339
SspMI CTAG 8 cut(s) 35, 996, 1029, 1131, 1914, 2300, 2727, 2756
StuI AGGCCT 1 cut(s) 2339
StyD4I CCNGG 5 cut(s) 311, 344, 1075, 1094, 2453
StyI CCWWGG 6 cut(s) 187, 839, 1590, 1906, 2311, 2331
TaaI ACNGT 6 cut(s) 658, 895, 946, 1852, 2382, 2994
TaiI ACGT 2 cut(s) 370, 1693
TaqI TCGA 9 cut(s) 90, 273, 408, 447, 545, 605, 707, 729, 2500
TaqII GACCGA 1 cut(s) 2831
TatI WGTACW 2 cut(s) 594, 2836
TauI GCSGC 3 cut(s) 240, 1091, 1889
TscAI CASTG 5 cut(s) 337, 661, 2366, 2385, 2754
TseFI GTSAC 3 cut(s) 10, 1948, 2749
Tsp45I GTSAC 3 cut(s) 10, 1948, 2749
TspDTI ATGAA 8 cut(s) 121, 456, 1001, 1230, 1406, 1682, 2130, 2198
TspGWI ACGGA 3 cut(s) 635, 875, 2638
TspRI CASTG 5 cut(s) 337, 661, 2366, 2385, 2754
Van91I CCANNNNNTGG 2 cut(s) 2768, 2870
Vha464I CTTAAG 2 cut(s) 977, 1040
VpaK11BI GGWCC 5 cut(s) 348, 802, 952, 1501, 2814
VspI ATTAAT 1 cut(s) 2220
XapI RAATTY 5 cut(s) 97, 443, 2076, 2102, 2795
XceI RCATGY 1 cut(s) 791
XhoI CTCGAG 2 cut(s) 272, 544
XmnI GAANNNNTTC 3 cut(s) 725, 1344, 2258
XspI CTAG 8 cut(s) 35, 996, 1029, 1131, 1914, 2300, 2727, 2756
Zsp2I ATGCAT 1 cut(s) 2852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.