RchiOBHm_Chr5g0050851

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000856
Physical Location & Seq
Reverse (-)
51573927 .. 51580752
6826 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32837

Sequence Viewer

Length: 3279 bp
ATGGTGTTCATCACATCTCCGAACCACAGAACTCTAAACCTGAATCTCAACCCAAAAACCACCACCCTCGAAACCCTCAAGCTTCAAATCCAGCAAAACTCCCACATCCCCATCTCCCAGCAGCGCCTCTTCATCTCCCAAAGCCTCCAAATCCTAACCCAACCCGACTCCACCCTCCTCTCCGACCTCGGCATCTCCCCCCTCTCTACCCTAACCCTCCACATCCCCTTATTCGGCGGCACCCAACCCCCCGCCGTCCCCAAACCCCGCCTCGACTTCCTCAACTCCAAGCCTCCCGCCAATTATGTCGCCGGCCTCGGCCGTGGCGCCACCGGATTCACCACGCGGTCCGATATCGGGCCGGCCCGCGCCGCTCCCGACTTGCCCGATCGGTCGGCGACGACCATCGGCGGGGCTGCCGGGGCGGCTCCGCCTGGGGTGGGCCGCGGGCGCGGGAAGCCGGAGGAAGAGGAGGAAGACGACGGCGAGGAGAAGGGTTACGATGAGAATCAGAAATTCGATGAGTTCGAAGGAAACGACGTCGGTTTGTTCGCGTCGGCGGAGTACGACGACGAGGATAAGGAGGCCGATGCGGTGTGGGAAGCCATTGAGCAGAGAATGGACTCGAGGAGAAAGGATAGGAGAGAAGCAAGGTTGAAACAAGAGATTGAGAAGTACAGAGCTTCGAATCCGAAAATCACAGAGCAGTTTGCGAATCTGAAGAGGCAGCTGTATACAGTTTCGGCTGAGGAATGGGATCGCATACCGGACATTGGGGATTACTCAGCCAGGAATAAGAAGAGGAAGTTTGAGAGCTTTGTGCCGGTGCCCGATACTCTTTTCGAGAAGGCTAGGCAGGAACAGGAGCATGTTACTGCATTGGACCCCAAGAGTAGGGCGGCCACCGGTACAGAGACGCCGTGGTCGCAAACGCCGGTTACGGATTTGACTGCAGTGGGGGATGGTAGAAGTACTGTGTTGTCTTTGAAGTTAGATAGGCTTTCGGATTCTGTTTCTGGGTTGACAGTTGTGGACCCTAAGGGTTATCTCACTGATCTTAAGAGTATGAAGATTACCAGTGATGCGGAGATTTCAGATATAAAGAAGGCTAGATTGCTGTTAAAGAGTGTGACGCAGACTAATCCGAAGCATCCACCGGGTTGGATTGCGGCTGCGAGGTTGGAGGAAGTGGCGGGGAAGATTCAGGCAGCAAGGCAATTGATCCAGAAAGGGTGTGAGGAATGTCCCAAGAGTGAGGATGTGTGGTTGGAGGCATGTAGGCTTGCCAGTCCTGACGGAGCCAAGGCTGTGATTGCTGCTGGAGTCAAGTCTATACCAAATTCAGTCAAGCTGTGGATGCAGGCGGCGAAATTGGAGCATGATGACTCGAATAAGAGTAGGGTGTTGAGGAAAGGGTTGGAACACATTCCTGATTCTGTTAGGCTGTGGAAGGCGGTGGTGGAGCTGGCAAATGAGGAGGATGCCAGACTTTTGCTTCAGAGGGCTGTGGAGTGTTGTCCCTTGCACATTGAGTTGTGGCTTGCACTAGCGAGATTGGAAACTTATGAAAATGCCAGGAAGGTCCTTAACAAGGCCCGCGAGAAGCTTTCGAAGGAGCCTGCCATTTGGATTACTGCAGCAAAGTTGGAAGAAGCTAATGGGAATACATCAACAGTTGGGAAGATTATTGAAAGGGGTATAAGGGCTTTGCAAAGAGAGGGGTTGGTGATTGATAGAGAAGCATGGATGAAGGAGGCTGAAGCTGCTGAGCGTGCAGGGTCTGTGGCAACTTGCCAAGCCATTATTCGGAACACAATTGGGATTGGTGTGGAGGAAGAAGATAGGAAGAGGACATGGGTTGCTGATGCAGAGGAGTGCAAGAAACGTGGTTCCATTGAAACAGCCAGAGCTATCTATGCGCATGCACTCACTGTCTTCTTGACTAAGAAGAGCATATGGCTTAAAGCAGCACAGCTTGAAAAGAGCCATGGGACAAGGGAATCTCTTGATGCCCTGCTTCGGAAAGCAGTTACCTACCGACCACAGGCTGAAGTCTTATGGCTTATGGGTGCTAAGGAGAAGTGGCTTGCTGGGGACGTGCCTGCTGCCCGTGCAATCCTCCAAGAAGCTTATGCTGCCATTCCGAATTCTGAAGAAATTTGGCTTGCTGCGTTTAAGCTTGAATTTGAGAATCATGAACCTGAGAGAGCTAGGATGCTTCTTGCCAAAGCCCGAGAAAGGGGAGGAACAGAGAGAGTATGGATGAAATCAGCAATCGTTGAAAGAGAATTAGGGAACCTCGATGAGGAGAGGAAGTTGCTTGCTGAAGGATTGAAGCGCTTCCCTTCGTTCTTTAAATTGTGGTTGATGCTTGGACAGCTAGAGGAACGGCTTAACCATTTGGAAAAAGCCAAGGAGGCTTATGACTCGGGTCTTAAGCACTGCTCCAATTCTATACCACTCTGGCTTTCTCTTGCAAATCTTGAAGAGAAGATGAATGGGCTGAGTAAAGCTCGTGCAGTTCTTACGATGGCCAGGAAGAAAAATCCTCAGAACCCTGAACTGTGGCTTGCTGCTGTTCGAGCTGAATTGAGGCATGGTAATAAGAGGGAAGCTGATATTTTGATGGCAAAGGCCTTGCAGGAGTGTTCCAATAGTGGTATCTTGTGGGCAGCTTCTATTGAGATGGTACCCCGTCCCCAAAGGAAGACCAAAAGTATGGATGCTCTCAAGAAATGTGATCACGATCCCCATGTCATTGCTGCTGTGGCCAAGTTATTCTGGCTCGACAGGAAGGTTGACAAGGCTAGGACTTGGCTCAACAGGGCAGTGACACTTGCCCCAGATATTGGAGATTTCTGGGCTTTATACTACAAATTTGAACTTCAGAATGGGACTGACGAGAACCAGAAGGATGTACTGAAAAGATGCATTGCTGCAGAACCAAAGCATGGTGAAAAATGGCAACCTATTTCGAAGGCAGTGGAGAACTCTCACCAACCAACTGAAGCTATCTTGAAGAAGGTGGTTGTTGCCCTTGGCAAGGAAGAGAGTGCAGCTGAAAATAATAGACAAGAAACAAGTATTGCCAGTCTCAATTGTGACGGAGGAAGAAGCAGCTCTAAGCGACTCAAGAGTAGGAGAAGAAGACAAACTGTTGTCTCATTCTTGATCTTTAATGAAGCTTCGAAAAGGGATATATACGTGGTAAAAGTCCAATACCGGTTGCATGTATCAAGTGCTCAACTAACCAAGAGAGGCCAACACCTTATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000387 GO:0000398 GO:0003674 GO:0003676 GO:0003712 GO:0003713 GO:0003723 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005684 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006403 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009628 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0015030 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0031047 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0034622 GO:0034641 GO:0035257 GO:0035258 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046540 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051252 GO:0051254 GO:0051427 GO:0060147 GO:0060148 GO:0060255 GO:0060964 GO:0060966 GO:0060968 GO:0065003 GO:0065007 GO:0070013 GO:0070920 GO:0071005 GO:0071011 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090351 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140110 GO:1901360 GO:1901363 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1903798 GO:1903800 GO:1990904 GO:2000112 GO:2000628 GO:2000630 GO:2000634 GO:2000636 GO:2000637 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
PRP1_N TPR_14 TPR_16 TPR_19 TPR_8 ubiquitin

Protein Analysis

1092

Amino Acids

122.31

Weight (kDa)

8.97

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP1_N PF06424 98 - 264 2.3e-52 PRP1 splicing factor, N-terminal
Suf PF05843 420 - 573 5.2e-07 Suppressor of forked protein (Suf)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 922
AatII GACGTC 1 cut(s) 543
Acc16I TGCGCA 1 cut(s) 1920
Acc65I GGTACC 1 cut(s) 2689
AccB1I GGYRCC 4 cut(s) 239, 326, 826, 2689
AccB7I CCANNNNNTGG 2 cut(s) 2849, 2951
AccBSI CCGCTC 1 cut(s) 374
AccI GTMKAC 1 cut(s) 734
AccII CGCG 6 cut(s) 346, 369, 447, 453, 554, 1599
AclWI GGATC 3 cut(s) 765, 1216, 2741
AcoI YGGCCR 4 cut(s) 319, 900, 2532, 2769
AcsI RAATTY 6 cut(s) 515, 1339, 2146, 2157, 2183, 2876
AcuI CTGAAG 8 cut(s) 740, 1481, 1779, 2070, 2172, 2346, 2870, 3027
AcyI GRCGYC 3 cut(s) 327, 540, 917
AfaI GTAC 6 cut(s) 566, 677, 910, 973, 2691, 2919
AfeI AGCGCT 1 cut(s) 2339
AflII CTTAAG 2 cut(s) 1058, 2435
AgeI ACCGGT 2 cut(s) 905, 3222
AjiI CACGTC 1 cut(s) 2098
AjnI CCWGG 4 cut(s) 433, 788, 1574, 2534
AjuI GAANNNNNNNTTGG 2 cut(s) 1788, 1820
Alw21I GWGCWC 1 cut(s) 3244
Alw26I GTCTC 3 cut(s) 908, 3098, 3166
AlwI GGATC 3 cut(s) 765, 1216, 2741
AlwNI CAGNNNCTG 1 cut(s) 1781
Ama87I CYCGRG 3 cut(s) 625, 2232, 2428
Aor51HI AGCGCT 1 cut(s) 2339
ApoI RAATTY 6 cut(s) 515, 1339, 2146, 2157, 2183, 2876
AsiGI ACCGGT 2 cut(s) 905, 3222
Asp700I GAANNNNTTC 2 cut(s) 1425, 2339
Asp718I GGTACC 1 cut(s) 2689
AspLEI GCGC 6 cut(s) 126, 329, 371, 453, 1921, 2340
AspS9I GGNCC 8 cut(s) 348, 359, 364, 442, 883, 1033, 1582, 1594
AsuC2I CCSGG 2 cut(s) 421, 1158
AsuHPI GGTGA 4 cut(s) 331, 1738, 2966, 2987
AsuII TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
AvaI CYCGRG 3 cut(s) 625, 2232, 2428
AvaII GGWCC 4 cut(s) 348, 883, 1033, 1582
AxyI CCTNAGG 1 cut(s) 1038
BaeGI GKGCMC 1 cut(s) 831
BalI TGGCCA 2 cut(s) 2534, 2771
BanI GGYRCC 4 cut(s) 239, 326, 826, 2689
BauI CACGAG 1 cut(s) 2514
BbsI GAAGAC 4 cut(s) 483, 1927, 2714, 3154
Bbv12I GWGCWC 1 cut(s) 3244
BbvCI CCTCAGC 1 cut(s) 747
BccI CCATC 6 cut(s) 119, 413, 956, 2524, 2620, 2680
BceAI ACGGC 5 cut(s) 239, 306, 499, 904, 2405
BciT130I CCWGG 4 cut(s) 435, 790, 1576, 2536
BclI TGATCA 1 cut(s) 2740
BcnI CCSGG 2 cut(s) 421, 1158
BcoDI GTCTC 3 cut(s) 908, 3098, 3166
BfaI CTAG 6 cut(s) 852, 1110, 1547, 2211, 2381, 2808
BfmI CTRYAG 3 cut(s) 951, 1635, 2937
BfoI RGCGCY 3 cut(s) 127, 330, 2341
BfrI CTTAAG 2 cut(s) 1058, 2435
BglI GCCNNNNNGGC 2 cut(s) 425, 2417
BlpI GCTNAGC 1 cut(s) 1767
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 6 cut(s) 421, 435, 790, 1158, 1576, 2536
Bme18I GGWCC 4 cut(s) 348, 883, 1033, 1582
BmeT110I CYCGRG 3 cut(s) 625, 2232, 2428
BmgBI CACGTC 1 cut(s) 2098
BmgT120I GGNCC 8 cut(s) 348, 359, 364, 442, 883, 1033, 1582, 1594
BmrFI CCNGG 6 cut(s) 421, 435, 790, 1158, 1576, 2536
BoxI GACNNNNGTC 1 cut(s) 2430
BpiI GAAGAC 4 cut(s) 483, 1927, 2714, 3154
BplI GAGNNNNNCTC 2 cut(s) 2497, 2529
BpmI CTGGAG 1 cut(s) 1341
Bpu10I CCTNAGC 2 cut(s) 747, 2073
Bpu1102I GCTNAGC 1 cut(s) 1767
Bpu14I TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
BpuEI CTTGAG 3 cut(s) 62, 2714, 3116
BpuMI CCSGG 2 cut(s) 421, 1158
BsaAI YACGTR 1 cut(s) 3205
BsaBI GATNNNNATC 2 cut(s) 507, 2745
BsaHI GRCGYC 3 cut(s) 327, 540, 917
BsaWI WCCGGW 4 cut(s) 332, 766, 905, 3222
BsaXI ACNNNNNCTCC 2 cut(s) 2300, 2330
Bse118I RCCGGY 6 cut(s) 311, 361, 823, 905, 934, 3222
Bse1I ACTGG 3 cut(s) 1077, 1287, 3090
Bse21I CCTNAGG 1 cut(s) 1038
Bse3DI GCAATG 2 cut(s) 2757, 2931
Bse8I GATNNNNATC 2 cut(s) 507, 2745
BseBI CCWGG 4 cut(s) 435, 790, 1576, 2536
BseJI GATNNNNATC 2 cut(s) 507, 2745
BseMI GCAATG 2 cut(s) 2757, 2931
BseMII CTCAG 6 cut(s) 738, 798, 1758, 2193, 2495, 2564
BseNI ACTGG 3 cut(s) 1077, 1287, 3090
BseRI GAGGAG 7 cut(s) 167, 485, 503, 643, 1490, 1886, 2321
BseSI GKGCMC 1 cut(s) 831
BseX3I CGGCCG 1 cut(s) 319
BseYI CCCAGC 2 cut(s) 117, 2090
BsgI GTGCAG 3 cut(s) 1794, 2538, 3075
Bsh1236I CGCG 6 cut(s) 346, 369, 447, 453, 554, 1599
Bsh1285I CGRYCG 3 cut(s) 322, 391, 395
BshNI GGYRCC 4 cut(s) 239, 326, 826, 2689
BshTI ACCGGT 2 cut(s) 905, 3222
BsiEI CGRYCG 3 cut(s) 322, 391, 395
BsiHKAI GWGCWC 1 cut(s) 3244
BsiHKCI CYCGRG 3 cut(s) 625, 2232, 2428
BslFI GGGAC 7 cut(s) 242, 1230, 1503, 2005, 2108, 2682, 2908
BsmAI GTCTC 3 cut(s) 908, 3098, 3166
BsmBI CGTCTC 1 cut(s) 908
BsmFI GGGAC 7 cut(s) 242, 1230, 1503, 2005, 2108, 2682, 2908
BsoBI CYCGRG 3 cut(s) 625, 2232, 2428
Bsp119I TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
Bsp1286I GDGCHC 2 cut(s) 831, 3244
Bsp143I GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
Bsp1720I GCTNAGC 1 cut(s) 1767
Bsp19I CCATGG 1 cut(s) 1987
BspCNI CTCAG 6 cut(s) 739, 797, 1759, 2194, 2496, 2563
BspFNI CGCG 6 cut(s) 346, 369, 447, 453, 554, 1599
BspHI TCATGA 1 cut(s) 2194
BspMAI CTGCAG 3 cut(s) 955, 1639, 2941
BspPI GGATC 3 cut(s) 765, 1216, 2741
BspQI GCTCTTC 1 cut(s) 1943
BspT104I TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
BspT107I GGYRCC 4 cut(s) 239, 326, 826, 2689
BspTI CTTAAG 2 cut(s) 1058, 2435
BsrBI CCGCTC 1 cut(s) 374
BsrDI GCAATG 2 cut(s) 2757, 2931
BsrFI RCCGGY 6 cut(s) 311, 361, 823, 905, 934, 3222
BsrI ACTGG 3 cut(s) 1077, 1287, 3090
BssAI RCCGGY 6 cut(s) 311, 361, 823, 905, 934, 3222
BssMI GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
BssNAI GTATAC 1 cut(s) 735
BssNI GRCGYC 3 cut(s) 327, 540, 917
BssSI CACGAG 1 cut(s) 2514
BssT1I CCWWGG 4 cut(s) 1302, 1987, 2412, 3037
Bst1107I GTATAC 1 cut(s) 735
Bst2BI CACGAG 1 cut(s) 2514
Bst2UI CCWGG 4 cut(s) 435, 790, 1576, 2536
Bst4CI ACNGT 7 cut(s) 739, 976, 1027, 1675, 1933, 2565, 3157
Bst6I CTCTTC 8 cut(s) 134, 462, 716, 794, 1841, 1943, 2481, 3042
BstACI GRCGYC 3 cut(s) 327, 540, 917
BstAFI CTTAAG 2 cut(s) 1058, 2435
BstBAI YACGTR 1 cut(s) 3205
BstBI TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
BstDSI CCRYGG 4 cut(s) 322, 445, 920, 1987
BstENI CCTNNNNNAGG 3 cut(s) 1589, 2303, 3041
BstFNI CGCG 6 cut(s) 346, 369, 447, 453, 554, 1599
BstH2I RGCGCY 3 cut(s) 127, 330, 2341
BstHHI GCGC 6 cut(s) 126, 329, 371, 453, 1921, 2340
BstKTI GATC 7 cut(s) 391, 760, 1057, 1224, 2743, 2749, 3174
BstMAI GTCTC 3 cut(s) 908, 3098, 3166
BstMBI GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
BstMCI CGRYCG 3 cut(s) 322, 391, 395
BstNI CCWGG 4 cut(s) 435, 790, 1576, 2536
BstNSI RCATGY 4 cut(s) 872, 1278, 1925, 3233
BstPAI GACNNNNGTC 1 cut(s) 2430
BstSCI CCNGG 6 cut(s) 419, 433, 788, 1156, 1574, 2534
BstSFI CTRYAG 3 cut(s) 951, 1635, 2937
BstSLI GKGCMC 1 cut(s) 831
BstUI CGCG 6 cut(s) 346, 369, 447, 453, 554, 1599
BstV2I GAAGAC 4 cut(s) 483, 1927, 2714, 3154
BstXI CCANNNNNNTGG 2 cut(s) 1161, 2719
BstZ17I GTATAC 1 cut(s) 735
BstZI CGGCCG 1 cut(s) 319
Bsu36I CCTNAGG 1 cut(s) 1038
BtgI CCRYGG 4 cut(s) 322, 445, 920, 1987
BtrI CACGTC 1 cut(s) 2098
BtsI GCAGTG 4 cut(s) 960, 2440, 2835, 2988
BtsIMutI CAGTG 7 cut(s) 960, 1050, 1084, 1929, 2440, 2835, 2988
CaiI CAGNNNCTG 1 cut(s) 1781
CciI TCATGA 1 cut(s) 2194
CfoI GCGC 6 cut(s) 126, 329, 371, 453, 1921, 2340
Cfr10I RCCGGY 6 cut(s) 311, 361, 823, 905, 934, 3222
Cfr13I GGNCC 8 cut(s) 348, 359, 364, 442, 883, 1033, 1582, 1594
Cfr42I CCGCGG 1 cut(s) 448
CpoI CGGWCCG 1 cut(s) 348
CseI GACGC 3 cut(s) 543, 925, 1141
Csp6I GTAC 6 cut(s) 565, 676, 909, 972, 2690, 2918
CspAI ACCGGT 2 cut(s) 905, 3222
CspCI CAANNNNNGTGG 2 cut(s) 1867, 1902
CspI CGGWCCG 1 cut(s) 348
CviQI GTAC 6 cut(s) 565, 676, 909, 972, 2690, 2918
DinI GGCGCC 1 cut(s) 328
DpnI GATC 7 cut(s) 390, 759, 1056, 1223, 2742, 2748, 3173
DpnII GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
DraI TTTAAA 1 cut(s) 2356
DrdI GACNNNNNNGTC 1 cut(s) 922
DseDI GACNNNNNNGTC 1 cut(s) 922
EaeI YGGCCR 4 cut(s) 319, 900, 2532, 2769
EagI CGGCCG 1 cut(s) 319
Eam1104I CTCTTC 8 cut(s) 134, 462, 716, 794, 1841, 1943, 2481, 3042
EarI CTCTTC 8 cut(s) 134, 462, 716, 794, 1841, 1943, 2481, 3042
EciI GGCGGA 2 cut(s) 420, 575
EclXI CGGCCG 1 cut(s) 319
Eco130I CCWWGG 4 cut(s) 1302, 1987, 2412, 3037
Eco147I AGGCCT 1 cut(s) 2636
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 4 cut(s) 348, 883, 1033, 1582
Eco47III AGCGCT 1 cut(s) 2339
Eco52I CGGCCG 1 cut(s) 319
Eco57I CTGAAG 8 cut(s) 740, 1481, 1779, 2070, 2172, 2346, 2870, 3027
Eco81I CCTNAGG 1 cut(s) 1038
Eco88I CYCGRG 3 cut(s) 625, 2232, 2428
EcoNI CCTNNNNNAGG 3 cut(s) 1589, 2303, 3041
EcoO109I RGGNCCY 1 cut(s) 1582
EcoRI GAATTC 1 cut(s) 2146
EcoRII CCWGG 4 cut(s) 433, 788, 1574, 2534
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 4 cut(s) 1302, 1987, 2412, 3037
EcoT22I ATGCAT 1 cut(s) 2933
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 4 cut(s) 1302, 1987, 2412, 3037
Esp3I CGTCTC 1 cut(s) 908
FaqI GGGAC 7 cut(s) 242, 1230, 1503, 2005, 2108, 2682, 2908
FauI CCCGC 9 cut(s) 259, 275, 304, 374, 404, 440, 446, 1186, 1604
FauNDI CATATG 1 cut(s) 1955
FbaI TGATCA 1 cut(s) 2740
FblI GTMKAC 1 cut(s) 734
FseI GGCCGGCC 1 cut(s) 365
FspAI RTGCGCAY 1 cut(s) 1920
FspBI CTAG 6 cut(s) 852, 1110, 1547, 2211, 2381, 2808
FspI TGCGCA 1 cut(s) 1920
GlaI GCGC 6 cut(s) 125, 328, 370, 452, 1920, 2339
GsaI CCCAGC 2 cut(s) 121, 2094
GsuI CTGGAG 1 cut(s) 1341
HaeII RGCGCY 3 cut(s) 127, 330, 2341
HgaI GACGC 3 cut(s) 543, 925, 1141
HhaI GCGC 6 cut(s) 126, 329, 371, 453, 1921, 2340
Hin1I GRCGYC 3 cut(s) 327, 540, 917
Hin6I GCGC 6 cut(s) 124, 327, 369, 451, 1919, 2338
HinP1I GCGC 6 cut(s) 124, 327, 369, 451, 1919, 2338
HincII GTYRAC 2 cut(s) 1023, 2800
HindII GTYRAC 2 cut(s) 1023, 2800
HindIII AAGCTT 5 cut(s) 80, 1604, 2127, 2177, 3183
HphI GGTGA 4 cut(s) 331, 1738, 2966, 2987
Hpy166II GTNNAC 4 cut(s) 735, 1023, 1033, 2800
Hpy8I GTNNAC 4 cut(s) 735, 1023, 1033, 2800
Hpy99I CGWCG 7 cut(s) 403, 485, 542, 545, 559, 572, 575
HpyCH4III ACNGT 7 cut(s) 739, 976, 1027, 1675, 1933, 2565, 3157
HpyCH4IV ACGT 4 cut(s) 540, 1885, 2097, 3204
HpySE526I ACGT 4 cut(s) 540, 1885, 2097, 3204
Hsp92I GRCGYC 3 cut(s) 327, 540, 917
HspAI GCGC 6 cut(s) 124, 327, 369, 451, 1919, 2338
KasI GGCGCC 1 cut(s) 326
KpnI GGTACC 1 cut(s) 2693
KroI GCCGGC 2 cut(s) 311, 361
KroNI GCCGGC 2 cut(s) 313, 363
Ksp22I TGATCA 1 cut(s) 2740
KspI CCGCGG 1 cut(s) 448
Kzo9I GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
LguI GCTCTTC 1 cut(s) 1943
LmnI GCTCC 8 cut(s) 379, 433, 865, 1298, 1375, 1462, 1615, 2450
MaeI CTAG 6 cut(s) 852, 1110, 1547, 2211, 2381, 2808
MaeII ACGT 4 cut(s) 540, 1885, 2097, 3204
MaeIII GTNAC 7 cut(s) 497, 871, 937, 1129, 2029, 2830, 3101
MalI GATC 7 cut(s) 390, 759, 1056, 1223, 2742, 2748, 3173
MbiI CCGCTC 1 cut(s) 374
MboI GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
MfeI CAATTG 3 cut(s) 1217, 1815, 3097
MhlI GDGCHC 2 cut(s) 831, 3244
MlsI TGGCCA 2 cut(s) 2534, 2771
MluNI TGGCCA 2 cut(s) 2534, 2771
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 6 cut(s) 161, 617, 1332, 1379, 2420, 3123
MmeI TCCRAC 6 cut(s) 207, 1142, 1161, 1248, 1398, 1626
Mox20I TGGCCA 2 cut(s) 2534, 2771
Mph1103I ATGCAT 1 cut(s) 2933
MroNI GCCGGC 2 cut(s) 311, 361
MroXI GAANNNNTTC 2 cut(s) 1425, 2339
MscI TGGCCA 2 cut(s) 2534, 2771
MseI TTAA 9 cut(s) 1059, 1121, 1587, 1962, 2175, 2355, 2394, 2436, 3177
Msp20I TGGCCA 2 cut(s) 2534, 2771
MspA1I CMGCKG 3 cut(s) 447, 730, 3059
MspCI CTTAAG 2 cut(s) 1058, 2435
MspR9I CCNGG 6 cut(s) 421, 435, 790, 1158, 1576, 2536
MunI CAATTG 3 cut(s) 1217, 1815, 3097
MvaI CCWGG 4 cut(s) 435, 790, 1576, 2536
MvnI CGCG 6 cut(s) 346, 369, 447, 453, 554, 1599
NaeI GCCGGC 2 cut(s) 313, 363
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 2 cut(s) 421, 1158
NcoI CCATGG 1 cut(s) 1987
NdeI CATATG 1 cut(s) 1955
NdeII GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
NgoMIV GCCGGC 2 cut(s) 311, 361
NmeAIII GCCGAG 2 cut(s) 168, 297
NmuCI GTSAC 3 cut(s) 1129, 2830, 3101
NsbI TGCGCA 1 cut(s) 1920
NsiI ATGCAT 1 cut(s) 2933
NspI RCATGY 4 cut(s) 872, 1278, 1925, 3233
NspV TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
PaeI GCATGC 1 cut(s) 1925
PaeR7I CTCGAG 1 cut(s) 625
PagI TCATGA 1 cut(s) 2194
PceI AGGCCT 1 cut(s) 2636
PciSI GCTCTTC 1 cut(s) 1943
PcsI WCGNNNNNNNCGW 2 cut(s) 525, 534
PdiI GCCGGC 2 cut(s) 313, 363
PdmI GAANNNNTTC 2 cut(s) 1425, 2339
PflMI CCANNNNNTGG 2 cut(s) 2849, 2951
PinAI ACCGGT 2 cut(s) 905, 3222
Ple19I CGATCG 1 cut(s) 391
PleI GAGTC 6 cut(s) 161, 617, 1331, 1379, 2420, 3123
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 6 cut(s) 161, 617, 1331, 1379, 2420, 3123
Ppu21I YACGTR 1 cut(s) 3205
PpuMI RGGWCCY 1 cut(s) 1582
PshAI GACNNNNGTC 1 cut(s) 2430
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 4 cut(s) 433, 788, 1574, 2534
PspFI CCCAGC 2 cut(s) 117, 2090
PspGI CCWGG 4 cut(s) 433, 788, 1574, 2534
PspPI GGNCC 8 cut(s) 348, 359, 364, 442, 883, 1033, 1582, 1594
PspPPI RGGWCCY 1 cut(s) 1582
PspXI VCTCGAGB 1 cut(s) 625
PstI CTGCAG 3 cut(s) 955, 1639, 2941
PstNI CAGNNNCTG 1 cut(s) 1781
PvuI CGATCG 1 cut(s) 391
PvuII CAGCTG 2 cut(s) 730, 3059
RigI GGCCGGCC 1 cut(s) 365
RsaI GTAC 6 cut(s) 566, 677, 910, 973, 2691, 2919
RsaNI GTAC 6 cut(s) 565, 676, 909, 972, 2690, 2918
Rsr2I CGGWCCG 1 cut(s) 348
RsrII CGGWCCG 1 cut(s) 348
SacII CCGCGG 1 cut(s) 448
SapI GCTCTTC 1 cut(s) 1943
SaqAI TTAA 9 cut(s) 1059, 1121, 1587, 1962, 2175, 2355, 2394, 2436, 3177
Sau3AI GATC 7 cut(s) 388, 757, 1054, 1221, 2740, 2746, 3171
Sau96I GGNCC 8 cut(s) 348, 359, 364, 442, 883, 1033, 1582, 1594
ScaI AGTACT 1 cut(s) 973
SchI GAGTC 6 cut(s) 161, 617, 1332, 1379, 2420, 3123
ScrFI CCNGG 6 cut(s) 421, 435, 790, 1158, 1576, 2536
SduI GDGCHC 2 cut(s) 831, 3244
SfcI CTRYAG 3 cut(s) 951, 1635, 2937
SfoI GGCGCC 1 cut(s) 328
Sfr274I CTCGAG 1 cut(s) 625
Sfr303I CCGCGG 1 cut(s) 448
SfuI TTCGAA 5 cut(s) 528, 686, 1610, 2975, 3188
SgrBI CCGCGG 1 cut(s) 448
SinI GGWCC 4 cut(s) 348, 883, 1033, 1582
SlaI CTCGAG 1 cut(s) 625
SmlI CTYRAG 6 cut(s) 77, 625, 1058, 2435, 2729, 3131
SmoI CTYRAG 6 cut(s) 77, 625, 1058, 2435, 2729, 3131
SphI GCATGC 1 cut(s) 1925
SseBI AGGCCT 1 cut(s) 2636
SspDI GGCGCC 1 cut(s) 326
SspMI CTAG 6 cut(s) 852, 1110, 1547, 2211, 2381, 2808
StuI AGGCCT 1 cut(s) 2636
StyD4I CCNGG 6 cut(s) 419, 433, 788, 1156, 1574, 2534
StyI CCWWGG 4 cut(s) 1302, 1987, 2412, 3037
TaaI ACNGT 7 cut(s) 739, 976, 1027, 1675, 1933, 2565, 3157
TaiI ACGT 4 cut(s) 543, 1888, 2100, 3207
TaqII GACCGA 1 cut(s) 381
TatI WGTACW 3 cut(s) 675, 971, 2917
TauI GCSGC 7 cut(s) 240, 374, 428, 447, 902, 1172, 1367
Tru1I TTAA 9 cut(s) 1059, 1121, 1587, 1962, 2175, 2355, 2394, 2436, 3177
Tru9I TTAA 9 cut(s) 1059, 1121, 1587, 1962, 2175, 2355, 2394, 2436, 3177
TscAI CASTG 7 cut(s) 960, 1057, 1084, 1936, 2447, 2835, 2988
TseFI GTSAC 3 cut(s) 1129, 2830, 3101
Tsp45I GTSAC 3 cut(s) 1129, 2830, 3101
TspDTI ATGAA 8 cut(s) 121, 1082, 1581, 1763, 2211, 2279, 2510, 3195
TspGWI ACGGA 3 cut(s) 956, 1311, 3120
TspRI CASTG 7 cut(s) 960, 1057, 1084, 1936, 2447, 2835, 2988
Van91I CCANNNNNTGG 2 cut(s) 2849, 2951
Vha464I CTTAAG 2 cut(s) 1058, 2435
VpaK11BI GGWCC 4 cut(s) 348, 883, 1033, 1582
XagI CCTNNNNNAGG 3 cut(s) 1589, 2303, 3041
XapI RAATTY 6 cut(s) 515, 1339, 2146, 2157, 2183, 2876
XceI RCATGY 4 cut(s) 872, 1278, 1925, 3233
XhoI CTCGAG 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 734
XmnI GAANNNNTTC 2 cut(s) 1425, 2339
XspI CTAG 6 cut(s) 852, 1110, 1547, 2211, 2381, 2808
ZraI GACGTC 1 cut(s) 541
ZrmI AGTACT 1 cut(s) 973
Zsp2I ATGCAT 1 cut(s) 2933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.