Rroxscaffold_1G00029850

pre-mRNA-processing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000856
Physical Location & Seq
Forward (+)
38837090 .. 38843849
6760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029850.1

Sequence Viewer

Length: 3291 bp
ATGGGAACGATCGGCCGTGCATCGCCGGAAAAGTCGTTTTCCCGCCGGCTCGGGTTTTCGTTTCGGGAAGGGCTTCGATCCACCCTGCGTGCGGCGGCGGTGCAAGAAGACACCGGAGGGAGCTTGGGCGGCCTGGTCCGATGGCGGTGGAGGCCGGTCCGGGTCGAAGGAGGCGGAAGGTGGTCAAACAACCGGGTGTCCATTGCTTACTATATGGAGAATTTCTTGCGAGCATATGTTATGAACCCTAAACCTGAATCTCAACCCAAAAACCACCACCCTCGAAACCCTCAAGCTTCAAATCCAGCAAAACTCCCACATCCCCATCTCCCAGCAGCGCCTCTTCATCTCCCAAAGCCTCCAAATCCTAACCCAAACCGATTCCACCCTCCTCTCCGACCTCGGCATCTCCCCCCTCTCTACCCTAACCCTCCACATCCCCTTATTCGGCGGCACCCAACCCCCGCCGTCCCCAAACCCCGCCTCGACTTCCTCAACTCCAAGCCTCCCGCCAATTATGTCGCCGGCCTCGGCCGTGGCGCCACCGGATTCACCACGCGGTCCGATATCGGGCCGGCCCGCGCCGCTCCCGACTTGCCCGATCGGTCGGCGACGACCATTGGCGGGGCTGCTGGGGCGGCTCCGCCTGGGGTGGGCCGCGGGCGCGGGAAGCCGGAGGAAGAGGAGGAAGACGACGGCGAGGAGAAGGGTTACGATGAGAATCAGAAATTCGATGAGTTCGAAGGAAACGACGTCGGTTTGTTCGCGTCGGCGGAGTACGACGACGAGGATAAGGAGGCCGATGCGGTGTGGGAAGCCATTGAGCAGAGAATGGACTCGAGGAGAAAGGATAGGAGAGAAGCAAGGTTGAAACAAGAGATTGAGAAGTACAGAGCTTCGAATCCGAAAATCACAGAGCAGTTTGCGAATCTGAAGAGGCAGCTGTATACAGTTTCGGCTGAGGAATGGGATCGCATACCGGACATTGGGGACTACTCAGCCAGGAATAAGAAGAGGAAGTTTGAGAGCTTTGTGCCGGTGCCGGATACTCTTTTCGAGAAGGCTAGGCAGGAACAGGAGCATGTTACTGCATTGGACCCCAAGAGTAGGGCGGCGACCGGTACAGAGACGCCGTGGTCGCAAACGCCGGTTACGGATTTGACTGCAGTGGGGGATGGTAGAAGTACTGTGTTGTCTTTGAAGTTAGATAGGCTTTCGGATTCTGTTTCTGGGTTGACAGTTGTGGACCCTAAGGGTTATCTCACTGATCTTAAGAGTATGAAGATTACCAGTGATGCGGAGATTTCGGATATAAAGAAGGCTAGATTGTTGTTAAAGAGTGTGACGCAGACTAATCCGAAGCATCCACCGGGTTGGATTGCGGCTGCAAGGTTGGAGGAAGTGGCGGGGAAGATTCAGGCAGCAAGGCAATTGATCCAGAAAGGGTGTGAGGAATGTCCCAAGAGTGAGGATGTGTGGTTGGAGGCATGTAGGCTTGCCAGTCCTGACGGAGCCAAGGCTGTGATTGCTGCTGGAGTCAAGTCTATACCAAATTCAGTCAAGCTGTGGATGCAGGCGGCAAAATTGGAGCATGATGACTCGAATAAGAGTAGGGTGTTGAGGAAAGGGTTGGAACACATTCCTGATTCTGTTAGGCTGTGGAAGGCGGTGGTGGAGCTGGCAAATGAGGAGGATGCCAGACTTTTGCTTCAGAGGGCTGTGGAGTGTTGTCCCTTGCACATTGAGTTGTGGCTTGCACTAGCGAGATTGGAAACTTATGAAAATGCCAGGAAGGTCCTTAACAAGGCCCGCGAGAAGCTTTCAAAGGAGCCTGCCATTTGGATTACTGCAGCAAAGTTGGAAGAAGCTAATGGGAATACATCAACAGTTGGGAAGATTATTGAAAGGGGTATAAGGGCTTTGCAAAGAGAGGGGTTGGTGATTGATAGAGAAGCATGGATGAAGGAGGCTGAAGCTGCCGAGCGTGCAGGGTCTGTGGCAACTTGCCAAGCCATTATTCGGAACACAATTGGGATTGGTGTGGAGGAAGAAGATAGGAAGAGGACATGGGTTGCTGATGCAGAGGAGTGCAAGAAACGTGGTTCCATTGAAACAGCCAGAGCTATCTATGCGCATGCACTCACTGTCTTCTTGACTAAGAAGAGCATATGGCTTAAAGCAGCACAGCTTGAAAAGAGCCATGGGACAAGGGAATCTCTTGATGCCCTGCTTCGGAAAGCAGTTACCTACCGACCACAGGCTGAAGTCTTATGGCTTATGGGTGCTAAGGAGAAGTGGCTTGCTGGGGATGTCCCTGCTGCCCGTGCAATCCTCCAAGAAGCTTATGCTGCCATTCCGAATTCTGAAGAAATTTGGCTTGCTGCGTTTAAGCTTGAATTTGAGAATCATGAACCTGAGAGAGCTAGGATGCTTCTTGCCAAAGCCCGAGAAAGGGGAGGAACAGAGAGAGTATGGATGAAATCAGCAATCGTTGAAAGAGAATTAGGGAACCTCGATGAGGAGAGGAAGTTGCTTGCTGAAGGATTGAAGCGCTTCCCTTCGTTCTTTAAATTGTGGTTGATGCTTGGACAGCTAGAGGAACGGCTCAACCATTTGGAAAAAGCCAAGGAGGCTTATGACTCGGGTCTTAAGCACTGCTCCAATTCTATACCACTCTGGCTTTCTCTTGCAAATCTTGAAGAGAAGATGAATGGGCTGAGTAAAGCTCGTGCAGTTCTTACGATGGCCAGGAAGAAAAATCCTCAGAACCCTGAACTGTGGCTTGCTGCTGTTCGAGCTGAATTGAGGCATGGTAATAAGAGGGAAGCTGATATTTTGATGGCAAAGGCCTTGCAGGAGTGTTCCAATAGCGGTATCTTGTGGGCAGCTTCTATTGAGATGGTACCCCGTCCCCAAAGGAAGACCAAAAGTATGGATGCTCTTAAGAAATGTGATCACGATCCCCATGTCATTGCTGCTGTGGCCAAGCTATTCTGGCTCGACAGGAAGGTTGACAAGGCTAGGACTTGGCTCAACAGGGCAGTGACACTTGCCCCAGATATTGGAGATTTCTGGGCTTTATACTACAAATTTGAACTTCAGAATGGGACTGACGAGAACCAGAAGGATGTACTGAAAAGATGCATTGCTGCAGAACCAAAGCATGGTGAAAAATGGCAACCTATTTCGAAGGCAGTGGAGAACTCTCACCAACCAACTGAAGCTATCTTGAAGAAGGCGGTTGTTGCCCTTGGCAAGGAAGAGAGTGCAGCTGAAAATAATAGACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000387 GO:0000398 GO:0003674 GO:0003676 GO:0003712 GO:0003713 GO:0003723 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005684 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006355 GO:0006357 GO:0006396 GO:0006397 GO:0006403 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008380 GO:0009266 GO:0009409 GO:0009628 GO:0009791 GO:0009845 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0015030 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0030532 GO:0031047 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0034622 GO:0034641 GO:0035257 GO:0035258 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044728 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0046540 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051252 GO:0051254 GO:0051427 GO:0060147 GO:0060148 GO:0060255 GO:0060964 GO:0060966 GO:0060968 GO:0065003 GO:0065007 GO:0070013 GO:0070920 GO:0071005 GO:0071011 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090351 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140110 GO:1901360 GO:1901363 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1903798 GO:1903800 GO:1990904 GO:2000112 GO:2000628 GO:2000630 GO:2000634 GO:2000636 GO:2000637 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
PRP1_N TPR_14 TPR_16 TPR_19 TPR_8 ubiquitin

Protein Analysis

1096

Amino Acids

122.85

Weight (kDa)

9.16

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRP1_N PF06424 169 - 335 2.3e-52 PRP1 splicing factor, N-terminal
Suf PF05843 491 - 644 5.2e-07 Suppressor of forked protein (Suf)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1135
AatII GACGTC 1 cut(s) 756
Acc16I TGCGCA 1 cut(s) 2133
Acc65I GGTACC 1 cut(s) 2902
AccB1I GGYRCC 4 cut(s) 453, 539, 1039, 2902
AccB7I CCANNNNNTGG 2 cut(s) 3062, 3164
AccBSI CCGCTC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 947
AccII CGCG 6 cut(s) 559, 582, 660, 666, 767, 1812
AclWI GGATC 4 cut(s) 72, 978, 1429, 2954
AcoI YGGCCR 4 cut(s) 13, 532, 2745, 2982
AcsI RAATTY 7 cut(s) 220, 728, 1552, 2359, 2370, 2396, 3089
AcuI CTGAAG 8 cut(s) 953, 1694, 1992, 2283, 2385, 2559, 3083, 3240
AcyI GRCGYC 3 cut(s) 540, 753, 1130
AfaI GTAC 6 cut(s) 779, 890, 1123, 1186, 2904, 3132
AfeI AGCGCT 1 cut(s) 2552
AflII CTTAAG 3 cut(s) 1271, 2648, 2942
AgeI ACCGGT 1 cut(s) 1118
AjnI CCWGG 5 cut(s) 132, 646, 1001, 1787, 2747
AjuI GAANNNNNNNTTGG 2 cut(s) 2001, 2033
Alw26I GTCTC 1 cut(s) 1121
AlwI GGATC 4 cut(s) 72, 978, 1429, 2954
AlwNI CAGNNNCTG 1 cut(s) 1994
Ama87I CYCGRG 4 cut(s) 50, 838, 2445, 2641
Aor51HI AGCGCT 1 cut(s) 2552
ApoI RAATTY 7 cut(s) 220, 728, 1552, 2359, 2370, 2396, 3089
AsiGI ACCGGT 1 cut(s) 1118
Asp700I GAANNNNTTC 3 cut(s) 72, 1638, 2552
Asp718I GGTACC 1 cut(s) 2902
AspLEI GCGC 6 cut(s) 340, 542, 584, 666, 2134, 2553
AsuC2I CCSGG 3 cut(s) 161, 194, 1371
AsuHPI GGTGA 4 cut(s) 544, 1951, 3179, 3200
AsuII TTCGAA 3 cut(s) 741, 899, 3188
AvaI CYCGRG 4 cut(s) 50, 838, 2445, 2641
AvaII GGWCC 6 cut(s) 136, 157, 561, 1096, 1246, 1795
AxyI CCTNAGG 1 cut(s) 1251
BalI TGGCCA 2 cut(s) 2747, 2984
BanI GGYRCC 4 cut(s) 453, 539, 1039, 2902
BauI CACGAG 1 cut(s) 2727
BbsI GAAGAC 4 cut(s) 114, 696, 2140, 2927
BbvCI CCTCAGC 1 cut(s) 960
BccI CCATC 6 cut(s) 135, 333, 1169, 2737, 2833, 2893
BceAI ACGGC 5 cut(s) 452, 519, 712, 1117, 2618
BciT130I CCWGG 5 cut(s) 134, 648, 1003, 1789, 2749
BciVI GTATCC 1 cut(s) 1039
BclI TGATCA 1 cut(s) 2953
BcnI CCSGG 3 cut(s) 161, 194, 1371
BcoDI GTCTC 1 cut(s) 1121
BfaI CTAG 6 cut(s) 1065, 1323, 1760, 2424, 2594, 3021
BfmI CTRYAG 3 cut(s) 1164, 1848, 3150
BfoI RGCGCY 3 cut(s) 341, 543, 2554
BfrI CTTAAG 3 cut(s) 1271, 2648, 2942
BfuI GTATCC 1 cut(s) 1039
BglI GCCNNNNNGGC 1 cut(s) 2630
BmcAI AGTACT 1 cut(s) 1186
Bme1390I CCNGG 8 cut(s) 134, 161, 194, 648, 1003, 1371, 1789, 2749
Bme18I GGWCC 6 cut(s) 136, 157, 561, 1096, 1246, 1795
BmeT110I CYCGRG 4 cut(s) 50, 838, 2445, 2641
BmrFI CCNGG 8 cut(s) 134, 161, 194, 648, 1003, 1371, 1789, 2749
BoxI GACNNNNGTC 1 cut(s) 2643
BpiI GAAGAC 4 cut(s) 114, 696, 2140, 2927
BplI GAGNNNNNCTC 2 cut(s) 2710, 2742
BpmI CTGGAG 1 cut(s) 1554
Bpu10I CCTNAGC 2 cut(s) 960, 2286
Bpu14I TTCGAA 3 cut(s) 741, 899, 3188
BpuEI CTTGAG 1 cut(s) 276
BpuMI CCSGG 3 cut(s) 161, 194, 1371
BsaBI GATNNNNATC 2 cut(s) 720, 2958
BsaHI GRCGYC 3 cut(s) 540, 753, 1130
BsaWI WCCGGW 4 cut(s) 113, 545, 979, 1118
BsaXI ACNNNNNCTCC 2 cut(s) 2513, 2543
Bse118I RCCGGY 7 cut(s) 45, 154, 524, 574, 1036, 1118, 1147
Bse1I ACTGG 2 cut(s) 1290, 1500
Bse21I CCTNAGG 1 cut(s) 1251
Bse3DI GCAATG 3 cut(s) 201, 2970, 3144
Bse8I GATNNNNATC 2 cut(s) 720, 2958
BseBI CCWGG 5 cut(s) 134, 648, 1003, 1789, 2749
BseJI GATNNNNATC 2 cut(s) 720, 2958
BseMI GCAATG 3 cut(s) 201, 2970, 3144
BseMII CTCAG 5 cut(s) 951, 1011, 2406, 2708, 2777
BseNI ACTGG 2 cut(s) 1290, 1500
BseRI GAGGAG 7 cut(s) 381, 698, 716, 856, 1703, 2099, 2534
BseX3I CGGCCG 2 cut(s) 13, 532
BseYI CCCAGC 3 cut(s) 331, 632, 2303
BsgI GTGCAG 3 cut(s) 2007, 2751, 3288
Bsh1236I CGCG 6 cut(s) 559, 582, 660, 666, 767, 1812
Bsh1285I CGRYCG 6 cut(s) 12, 16, 535, 604, 608, 1119
BshNI GGYRCC 4 cut(s) 453, 539, 1039, 2902
BshTI ACCGGT 1 cut(s) 1118
BsiEI CGRYCG 6 cut(s) 12, 16, 535, 604, 608, 1119
BsiHKCI CYCGRG 4 cut(s) 50, 838, 2445, 2641
BslFI GGGAC 8 cut(s) 455, 1004, 1443, 1716, 2218, 2297, 2895, 3121
BsmAI GTCTC 1 cut(s) 1121
BsmBI CGTCTC 1 cut(s) 1121
BsmFI GGGAC 8 cut(s) 455, 1004, 1443, 1716, 2218, 2297, 2895, 3121
BsoBI CYCGRG 4 cut(s) 50, 838, 2445, 2641
Bsp119I TTCGAA 3 cut(s) 741, 899, 3188
Bsp143I GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
Bsp19I CCATGG 1 cut(s) 2200
BspCNI CTCAG 5 cut(s) 952, 1010, 2407, 2709, 2776
BspFNI CGCG 6 cut(s) 559, 582, 660, 666, 767, 1812
BspHI TCATGA 1 cut(s) 2407
BspMAI CTGCAG 3 cut(s) 1168, 1852, 3154
BspPI GGATC 4 cut(s) 72, 978, 1429, 2954
BspQI GCTCTTC 1 cut(s) 2156
BspT104I TTCGAA 3 cut(s) 741, 899, 3188
BspT107I GGYRCC 4 cut(s) 453, 539, 1039, 2902
BspTI CTTAAG 3 cut(s) 1271, 2648, 2942
BsrBI CCGCTC 1 cut(s) 587
BsrDI GCAATG 3 cut(s) 201, 2970, 3144
BsrFI RCCGGY 7 cut(s) 45, 154, 524, 574, 1036, 1118, 1147
BsrI ACTGG 2 cut(s) 1290, 1500
BssAI RCCGGY 7 cut(s) 45, 154, 524, 574, 1036, 1118, 1147
BssMI GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
BssNAI GTATAC 1 cut(s) 948
BssNI GRCGYC 3 cut(s) 540, 753, 1130
BssSI CACGAG 1 cut(s) 2727
BssT1I CCWWGG 4 cut(s) 1515, 2200, 2625, 3250
Bst1107I GTATAC 1 cut(s) 948
Bst2BI CACGAG 1 cut(s) 2727
Bst2UI CCWGG 5 cut(s) 134, 648, 1003, 1789, 2749
Bst4CI ACNGT 6 cut(s) 952, 1189, 1240, 1888, 2146, 2778
Bst6I CTCTTC 8 cut(s) 348, 675, 929, 1007, 2054, 2156, 2694, 3255
BstACI GRCGYC 3 cut(s) 540, 753, 1130
BstAFI CTTAAG 3 cut(s) 1271, 2648, 2942
BstBI TTCGAA 3 cut(s) 741, 899, 3188
BstDEI CTNAG 8 cut(s) 960, 997, 1251, 2157, 2286, 2415, 2717, 2763
BstDSI CCRYGG 4 cut(s) 535, 658, 1133, 2200
BstENI CCTNNNNNAGG 3 cut(s) 1802, 2516, 3254
BstFNI CGCG 6 cut(s) 559, 582, 660, 666, 767, 1812
BstH2I RGCGCY 3 cut(s) 341, 543, 2554
BstHHI GCGC 6 cut(s) 340, 542, 584, 666, 2134, 2553
BstKTI GATC 8 cut(s) 12, 80, 604, 973, 1270, 1437, 2956, 2962
BstMAI GTCTC 1 cut(s) 1121
BstMBI GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
BstMCI CGRYCG 6 cut(s) 12, 16, 535, 604, 608, 1119
BstNI CCWGG 5 cut(s) 134, 648, 1003, 1789, 2749
BstNSI RCATGY 3 cut(s) 1085, 1491, 2138
BstPAI GACNNNNGTC 1 cut(s) 2643
BstSCI CCNGG 8 cut(s) 132, 159, 192, 646, 1001, 1369, 1787, 2747
BstSFI CTRYAG 3 cut(s) 1164, 1848, 3150
BstUI CGCG 6 cut(s) 559, 582, 660, 666, 767, 1812
BstV2I GAAGAC 4 cut(s) 114, 696, 2140, 2927
BstXI CCANNNNNNTGG 2 cut(s) 1374, 2932
BstZ17I GTATAC 1 cut(s) 948
BstZI CGGCCG 2 cut(s) 13, 532
Bsu36I CCTNAGG 1 cut(s) 1251
BsuI GTATCC 1 cut(s) 1039
BtgI CCRYGG 4 cut(s) 535, 658, 1133, 2200
BtgZI GCGATG 1 cut(s) 6
BtsI GCAGTG 4 cut(s) 1173, 2653, 3048, 3201
BtsIMutI CAGTG 7 cut(s) 1173, 1263, 1297, 2142, 2653, 3048, 3201
CaiI CAGNNNCTG 1 cut(s) 1994
CciI TCATGA 1 cut(s) 2407
CfoI GCGC 6 cut(s) 340, 542, 584, 666, 2134, 2553
Cfr10I RCCGGY 7 cut(s) 45, 154, 524, 574, 1036, 1118, 1147
Cfr42I CCGCGG 1 cut(s) 661
CpoI CGGWCCG 2 cut(s) 157, 561
CseI GACGC 3 cut(s) 756, 1138, 1354
Csp6I GTAC 6 cut(s) 778, 889, 1122, 1185, 2903, 3131
CspAI ACCGGT 1 cut(s) 1118
CspCI CAANNNNNGTGG 2 cut(s) 2080, 2115
CspI CGGWCCG 2 cut(s) 157, 561
CviQI GTAC 6 cut(s) 778, 889, 1122, 1185, 2903, 3131
DdeI CTNAG 8 cut(s) 960, 997, 1251, 2157, 2286, 2415, 2717, 2763
DinI GGCGCC 1 cut(s) 541
DpnI GATC 8 cut(s) 11, 79, 603, 972, 1269, 1436, 2955, 2961
DpnII GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
DraI TTTAAA 1 cut(s) 2569
DrdI GACNNNNNNGTC 1 cut(s) 1135
DseDI GACNNNNNNGTC 1 cut(s) 1135
EaeI YGGCCR 4 cut(s) 13, 532, 2745, 2982
EagI CGGCCG 2 cut(s) 13, 532
Eam1104I CTCTTC 8 cut(s) 348, 675, 929, 1007, 2054, 2156, 2694, 3255
EarI CTCTTC 8 cut(s) 348, 675, 929, 1007, 2054, 2156, 2694, 3255
EciI GGCGGA 3 cut(s) 189, 633, 788
EclXI CGGCCG 2 cut(s) 13, 532
Eco130I CCWWGG 4 cut(s) 1515, 2200, 2625, 3250
Eco147I AGGCCT 1 cut(s) 2849
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 6 cut(s) 136, 157, 561, 1096, 1246, 1795
Eco47III AGCGCT 1 cut(s) 2552
Eco52I CGGCCG 2 cut(s) 13, 532
Eco57I CTGAAG 8 cut(s) 953, 1694, 1992, 2283, 2385, 2559, 3083, 3240
Eco81I CCTNAGG 1 cut(s) 1251
Eco88I CYCGRG 4 cut(s) 50, 838, 2445, 2641
EcoNI CCTNNNNNAGG 3 cut(s) 1802, 2516, 3254
EcoO109I RGGNCCY 1 cut(s) 1795
EcoRI GAATTC 1 cut(s) 2359
EcoRII CCWGG 5 cut(s) 132, 646, 1001, 1787, 2747
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 4 cut(s) 1515, 2200, 2625, 3250
EcoT22I ATGCAT 1 cut(s) 3146
EgeI GGCGCC 1 cut(s) 541
EheI GGCGCC 1 cut(s) 541
ErhI CCWWGG 4 cut(s) 1515, 2200, 2625, 3250
Esp3I CGTCTC 1 cut(s) 1121
FaqI GGGAC 8 cut(s) 455, 1004, 1443, 1716, 2218, 2297, 2895, 3121
FauNDI CATATG 2 cut(s) 235, 2168
FbaI TGATCA 1 cut(s) 2953
FblI GTMKAC 1 cut(s) 947
FseI GGCCGGCC 1 cut(s) 578
FspAI RTGCGCAY 1 cut(s) 2133
FspBI CTAG 6 cut(s) 1065, 1323, 1760, 2424, 2594, 3021
FspI TGCGCA 1 cut(s) 2133
GlaI GCGC 6 cut(s) 339, 541, 583, 665, 2133, 2552
GsaI CCCAGC 3 cut(s) 335, 636, 2307
GsuI CTGGAG 1 cut(s) 1554
HaeII RGCGCY 3 cut(s) 341, 543, 2554
HgaI GACGC 3 cut(s) 756, 1138, 1354
HhaI GCGC 6 cut(s) 340, 542, 584, 666, 2134, 2553
Hin1I GRCGYC 3 cut(s) 540, 753, 1130
Hin6I GCGC 6 cut(s) 338, 540, 582, 664, 2132, 2551
HinP1I GCGC 6 cut(s) 338, 540, 582, 664, 2132, 2551
HincII GTYRAC 2 cut(s) 1236, 3013
HindII GTYRAC 2 cut(s) 1236, 3013
HindIII AAGCTT 4 cut(s) 294, 1817, 2340, 2390
HphI GGTGA 4 cut(s) 544, 1951, 3179, 3200
Hpy166II GTNNAC 4 cut(s) 948, 1236, 1246, 3013
Hpy8I GTNNAC 4 cut(s) 948, 1236, 1246, 3013
Hpy99I CGWCG 7 cut(s) 616, 698, 755, 758, 772, 785, 788
HpyCH4III ACNGT 6 cut(s) 952, 1189, 1240, 1888, 2146, 2778
HpyCH4IV ACGT 2 cut(s) 753, 2098
HpyF3I CTNAG 8 cut(s) 960, 997, 1251, 2157, 2286, 2415, 2717, 2763
HpySE526I ACGT 2 cut(s) 753, 2098
Hsp92I GRCGYC 3 cut(s) 540, 753, 1130
HspAI GCGC 6 cut(s) 338, 540, 582, 664, 2132, 2551
KasI GGCGCC 1 cut(s) 539
KpnI GGTACC 1 cut(s) 2906
KroI GCCGGC 3 cut(s) 45, 524, 574
KroNI GCCGGC 3 cut(s) 47, 526, 576
Ksp22I TGATCA 1 cut(s) 2953
KspI CCGCGG 1 cut(s) 661
Kzo9I GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
LguI GCTCTTC 1 cut(s) 2156
LmnI GCTCC 9 cut(s) 120, 592, 646, 1078, 1511, 1588, 1675, 1828, 2663
MaeI CTAG 6 cut(s) 1065, 1323, 1760, 2424, 2594, 3021
MaeII ACGT 2 cut(s) 753, 2098
MaeIII GTNAC 6 cut(s) 710, 1084, 1150, 1342, 2242, 3043
MalI GATC 8 cut(s) 11, 79, 603, 972, 1269, 1436, 2955, 2961
MbiI CCGCTC 1 cut(s) 587
MboI GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
MfeI CAATTG 2 cut(s) 1430, 2028
MlsI TGGCCA 2 cut(s) 2747, 2984
MluNI TGGCCA 2 cut(s) 2747, 2984
Mly113I GGCGCC 1 cut(s) 540
MlyI GAGTC 4 cut(s) 830, 1545, 1592, 2633
MmeI TCCRAC 6 cut(s) 421, 1355, 1374, 1461, 1611, 1839
Mox20I TGGCCA 2 cut(s) 2747, 2984
Mph1103I ATGCAT 1 cut(s) 3146
MroNI GCCGGC 3 cut(s) 45, 524, 574
MroXI GAANNNNTTC 3 cut(s) 72, 1638, 2552
MscI TGGCCA 2 cut(s) 2747, 2984
MseI TTAA 8 cut(s) 1272, 1334, 1800, 2175, 2388, 2568, 2649, 2943
Msp20I TGGCCA 2 cut(s) 2747, 2984
MspA1I CMGCKG 3 cut(s) 660, 943, 3272
MspCI CTTAAG 3 cut(s) 1271, 2648, 2942
MspR9I CCNGG 8 cut(s) 134, 161, 194, 648, 1003, 1371, 1789, 2749
MunI CAATTG 2 cut(s) 1430, 2028
MvaI CCWGG 5 cut(s) 134, 648, 1003, 1789, 2749
MvnI CGCG 6 cut(s) 559, 582, 660, 666, 767, 1812
NaeI GCCGGC 3 cut(s) 47, 526, 576
NarI GGCGCC 1 cut(s) 540
NciI CCSGG 3 cut(s) 161, 194, 1371
NcoI CCATGG 1 cut(s) 2200
NdeI CATATG 2 cut(s) 235, 2168
NdeII GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
NgoMIV GCCGGC 3 cut(s) 45, 524, 574
NmeAIII GCCGAG 3 cut(s) 382, 510, 2005
NmuCI GTSAC 2 cut(s) 1342, 3043
NsbI TGCGCA 1 cut(s) 2133
NsiI ATGCAT 1 cut(s) 3146
NspI RCATGY 3 cut(s) 1085, 1491, 2138
NspV TTCGAA 3 cut(s) 741, 899, 3188
PaeI GCATGC 1 cut(s) 2138
PaeR7I CTCGAG 1 cut(s) 838
PagI TCATGA 1 cut(s) 2407
PceI AGGCCT 1 cut(s) 2849
PciSI GCTCTTC 1 cut(s) 2156
PcsI WCGNNNNNNNCGW 2 cut(s) 738, 747
PdiI GCCGGC 3 cut(s) 47, 526, 576
PdmI GAANNNNTTC 3 cut(s) 72, 1638, 2552
PflMI CCANNNNNTGG 2 cut(s) 3062, 3164
PinAI ACCGGT 1 cut(s) 1118
Ple19I CGATCG 2 cut(s) 12, 604
PleI GAGTC 4 cut(s) 830, 1544, 1592, 2633
PluTI GGCGCC 1 cut(s) 543
PpsI GAGTC 4 cut(s) 830, 1544, 1592, 2633
PpuMI RGGWCCY 1 cut(s) 1795
PshAI GACNNNNGTC 1 cut(s) 2643
Psp5II RGGWCCY 1 cut(s) 1795
Psp6I CCWGG 5 cut(s) 132, 646, 1001, 1787, 2747
PspFI CCCAGC 3 cut(s) 331, 632, 2303
PspGI CCWGG 5 cut(s) 132, 646, 1001, 1787, 2747
PspPPI RGGWCCY 1 cut(s) 1795
PspXI VCTCGAGB 1 cut(s) 838
PstI CTGCAG 3 cut(s) 1168, 1852, 3154
PstNI CAGNNNCTG 1 cut(s) 1994
PvuI CGATCG 2 cut(s) 12, 604
PvuII CAGCTG 2 cut(s) 943, 3272
RigI GGCCGGCC 1 cut(s) 578
RsaI GTAC 6 cut(s) 779, 890, 1123, 1186, 2904, 3132
RsaNI GTAC 6 cut(s) 778, 889, 1122, 1185, 2903, 3131
Rsr2I CGGWCCG 2 cut(s) 157, 561
RsrII CGGWCCG 2 cut(s) 157, 561
SacII CCGCGG 1 cut(s) 661
SapI GCTCTTC 1 cut(s) 2156
SaqAI TTAA 8 cut(s) 1272, 1334, 1800, 2175, 2388, 2568, 2649, 2943
Sau3AI GATC 8 cut(s) 9, 77, 601, 970, 1267, 1434, 2953, 2959
ScaI AGTACT 1 cut(s) 1186
SchI GAGTC 4 cut(s) 830, 1545, 1592, 2633
ScrFI CCNGG 8 cut(s) 134, 161, 194, 648, 1003, 1371, 1789, 2749
SfcI CTRYAG 3 cut(s) 1164, 1848, 3150
SfoI GGCGCC 1 cut(s) 541
Sfr274I CTCGAG 1 cut(s) 838
Sfr303I CCGCGG 1 cut(s) 661
SfuI TTCGAA 3 cut(s) 741, 899, 3188
SgrBI CCGCGG 1 cut(s) 661
SinI GGWCC 6 cut(s) 136, 157, 561, 1096, 1246, 1795
SlaI CTCGAG 1 cut(s) 838
SmlI CTYRAG 5 cut(s) 291, 838, 1271, 2648, 2942
SmoI CTYRAG 5 cut(s) 291, 838, 1271, 2648, 2942
SphI GCATGC 1 cut(s) 2138
SseBI AGGCCT 1 cut(s) 2849
SspDI GGCGCC 1 cut(s) 539
SspMI CTAG 6 cut(s) 1065, 1323, 1760, 2424, 2594, 3021
StuI AGGCCT 1 cut(s) 2849
StyD4I CCNGG 8 cut(s) 132, 159, 192, 646, 1001, 1369, 1787, 2747
StyI CCWWGG 4 cut(s) 1515, 2200, 2625, 3250
TaaI ACNGT 6 cut(s) 952, 1189, 1240, 1888, 2146, 2778
TaiI ACGT 2 cut(s) 756, 2101
TaqII GACCGA 1 cut(s) 594
TatI WGTACW 3 cut(s) 888, 1184, 3130
Tru1I TTAA 8 cut(s) 1272, 1334, 1800, 2175, 2388, 2568, 2649, 2943
Tru9I TTAA 8 cut(s) 1272, 1334, 1800, 2175, 2388, 2568, 2649, 2943
TscAI CASTG 7 cut(s) 1173, 1270, 1297, 2149, 2660, 3048, 3201
TseFI GTSAC 2 cut(s) 1342, 3043
Tsp45I GTSAC 2 cut(s) 1342, 3043
TspDTI ATGAA 8 cut(s) 257, 335, 1295, 1794, 1976, 2424, 2492, 2723
TspGWI ACGGA 2 cut(s) 1169, 1524
TspRI CASTG 7 cut(s) 1173, 1270, 1297, 2149, 2660, 3048, 3201
Van91I CCANNNNNTGG 2 cut(s) 3062, 3164
Vha464I CTTAAG 3 cut(s) 1271, 2648, 2942
VpaK11BI GGWCC 6 cut(s) 136, 157, 561, 1096, 1246, 1795
XagI CCTNNNNNAGG 3 cut(s) 1802, 2516, 3254
XapI RAATTY 7 cut(s) 220, 728, 1552, 2359, 2370, 2396, 3089
XceI RCATGY 3 cut(s) 1085, 1491, 2138
XhoI CTCGAG 1 cut(s) 838
XmiI GTMKAC 1 cut(s) 947
XmnI GAANNNNTTC 3 cut(s) 72, 1638, 2552
XspI CTAG 6 cut(s) 1065, 1323, 1760, 2424, 2594, 3021
ZraI GACGTC 1 cut(s) 754
ZrmI AGTACT 1 cut(s) 1186
Zsp2I ATGCAT 1 cut(s) 3146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.