RchiOBHm_Chr4g0385301

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000849
Physical Location & Seq
Reverse (-)
767375 .. 768537
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 789 bp
ATGGACATGATCAGCATGTTGCCTGTGGAGTGCATCTCCCTCATCATTTCCTTTACATCTCCGCGCGATGCCTGCAGATCATCAGTAGTCTCTCCTGTCTTGAGAATAGCTGGAGATTCTGATTTTGTCTGGGAGAGGTTTCTGCCTCAAGATTATAAGCAAATCATTTTGCAATCCCCGTCATCGTCGTCTTTGAACTCCATGTCCAAGAAGGATCTCTACTTTAGTCTTTGCAGTCACCCCATCAACATAAGCAATCGTAACATGAGCTTTGCACTAGAAAAGCAAAGCGGCAAGAAATGTTATATGGTATGCGCAAGAGGGCTTACAATTGCATGGGGAGATACACCCGCTTATTGGCATTGGATAACTCTACTACACTCCAGGTTTGCTGAAGTGGCTGAGCTCAAACGTGTATGGTGGCTGGACATCAAAGCATGCATAGAGACTAAGGATTTATCCCCAGGAACAACCTATGCAGCTTACCTTGTTTATCAGCTCTCACAACACAGATATGGACTTGAATCGCCTCTTGTGTCGCGTATCAGTTATCAACAAAGTGACGTAGTGACTGTGCATCGTGTGATCCTAGACCCTAGGCCTCCGCTACCGCGAGCTCGAGACAGAGGAGATGGGTGGATAGAGGTTGAGATAGGTGAATTTTTCATTGAACAGGGAGACGATGATGATATTGTAGAGTGCAGTGTAAAGGAAGTCAGTAACTCTAAGCGCGGCCTCATCGTTGAAGGTATTGAGGTGAGGCCTAAAGGCAGGGCTGGCCCTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like PP2

Protein Analysis

262

Amino Acids

29.63

Weight (kDa)

6.23

Isoelectric Point (pI)

65.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 99 - 255 3.9e-47 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
Acc16I TGCGCA 1 cut(s) 316
AccII CGCG 5 cut(s) 64, 66, 541, 613, 732
AciI CCGC 6 cut(s) 62, 291, 351, 605, 611, 732
AclWI GGATC 2 cut(s) 222, 580
AcsI RAATTY 1 cut(s) 659
AcuI CTGAAG 1 cut(s) 414
AfiI CCNNNNNNNGG 1 cut(s) 357
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 4 cut(s) 196, 524, 671, 746
AjnI CCWGG 3 cut(s) 383, 463, 781
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
AluBI AGCT 6 cut(s) 110, 270, 406, 482, 499, 617
AluI AGCT 6 cut(s) 110, 270, 406, 482, 499, 617
Alw21I GWGCWC 2 cut(s) 408, 619
Alw26I GTCTC 4 cut(s) 94, 440, 615, 672
AlwI GGATC 2 cut(s) 222, 580
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 4 cut(s) 599, 733, 761, 778
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 659
AspA2I CCTAGG 1 cut(s) 596
AspLEI GCGC 3 cut(s) 66, 317, 732
AspS9I GGNCC 1 cut(s) 779
AsuHPI GGTGA 3 cut(s) 230, 668, 769
AvaI CYCGRG 1 cut(s) 618
AvrII CCTAGG 1 cut(s) 596
BanII GRGCYC 2 cut(s) 408, 619
BarI GAAGNNNNNNTAC 2 cut(s) 203, 235
Bbv12I GWGCWC 2 cut(s) 408, 619
BbvI GCAGC 1 cut(s) 491
BccI CCATC 2 cut(s) 251, 626
BciT130I CCWGG 3 cut(s) 385, 465, 783
BclI TGATCA 1 cut(s) 9
BcoDI GTCTC 4 cut(s) 94, 440, 615, 672
BfaI CTAG 3 cut(s) 278, 590, 597
BfmI CTRYAG 1 cut(s) 73
BisI GCNGC 3 cut(s) 292, 480, 733
BlnI CCTAGG 1 cut(s) 596
BlpI GCTNAGC 1 cut(s) 402
BlsI GCNGC 3 cut(s) 293, 481, 734
Bme1390I CCNGG 3 cut(s) 385, 465, 783
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 779
BmrFI CCNGG 3 cut(s) 385, 465, 783
BmsI GCATC 3 cut(s) 42, 58, 586
BplI GAGNNNNNCTC 2 cut(s) 20, 52
BpmI CTGGAG 2 cut(s) 132, 367
Bpu1102I GCTNAGC 1 cut(s) 402
BpuEI CTTGAG 2 cut(s) 121, 132
BsaJI CCNNGG 4 cut(s) 463, 596, 781, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 357
BseBI CCWGG 3 cut(s) 385, 465, 783
BseDI CCNNGG 4 cut(s) 463, 596, 781, 782
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 393
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 491
BsgI GTGCAG 1 cut(s) 721
Bsh1236I CGCG 5 cut(s) 64, 66, 541, 613, 732
BshFI GGCC 4 cut(s) 601, 735, 763, 780
BsiHKAI GWGCWC 2 cut(s) 408, 619
BsiHKCI CYCGRG 1 cut(s) 618
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 4 cut(s) 94, 440, 615, 672
BsmBI CGTCTC 1 cut(s) 672
BsnI GGCC 4 cut(s) 601, 735, 763, 780
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 2 cut(s) 408, 619
Bsp143I GATC 4 cut(s) 9, 77, 214, 585
Bsp1720I GCTNAGC 1 cut(s) 402
BspACI CCGC 6 cut(s) 62, 291, 351, 605, 611, 732
BspANI GGCC 4 cut(s) 601, 735, 763, 780
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 5 cut(s) 64, 66, 541, 613, 732
BspMAI CTGCAG 1 cut(s) 77
BspPI GGATC 2 cut(s) 222, 580
BssECI CCNNGG 4 cut(s) 463, 596, 781, 782
BssMI GATC 4 cut(s) 9, 77, 214, 585
BssT1I CCWWGG 1 cut(s) 596
Bst2UI CCWGG 3 cut(s) 385, 465, 783
Bst4CI ACNGT 1 cut(s) 574
BstC8I GCNNGC 4 cut(s) 73, 439, 615, 778
BstDEI CTNAG 3 cut(s) 402, 450, 726
BstFNI CGCG 5 cut(s) 64, 66, 541, 613, 732
BstHHI GCGC 3 cut(s) 66, 317, 732
BstKTI GATC 4 cut(s) 12, 80, 217, 588
BstMAI GTCTC 4 cut(s) 94, 440, 615, 672
BstMBI GATC 4 cut(s) 9, 77, 214, 585
BstMWI GCNNNNNNNGC 3 cut(s) 72, 398, 777
BstNI CCWGG 3 cut(s) 385, 465, 783
BstNSI RCATGY 2 cut(s) 19, 441
BstSCI CCNGG 3 cut(s) 383, 463, 781
BstSFI CTRYAG 1 cut(s) 73
BstUI CGCG 5 cut(s) 64, 66, 541, 613, 732
BstV1I GCAGC 1 cut(s) 491
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 4 cut(s) 601, 735, 763, 780
BtgZI GCGATG 1 cut(s) 81
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 1 cut(s) 709
Cac8I GCNNGC 4 cut(s) 73, 439, 615, 778
CfoI GCGC 3 cut(s) 66, 317, 732
Cfr13I GGNCC 1 cut(s) 779
CviAII CATG 6 cut(s) 7, 16, 202, 265, 336, 438
DdeI CTNAG 3 cut(s) 402, 450, 726
DpnI GATC 4 cut(s) 11, 79, 216, 587
DpnII GATC 4 cut(s) 9, 77, 214, 585
Ecl136II GAGCTC 2 cut(s) 406, 617
Eco130I CCWWGG 1 cut(s) 596
Eco147I AGGCCT 2 cut(s) 601, 763
Eco24I GRGCYC 2 cut(s) 408, 619
Eco53kI GAGCTC 2 cut(s) 406, 617
Eco57I CTGAAG 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 618
EcoICRI GAGCTC 2 cut(s) 406, 617
EcoRII CCWGG 3 cut(s) 383, 463, 781
EcoT14I CCWWGG 1 cut(s) 596
EcoT22I ATGCAT 1 cut(s) 443
EcoT38I GRGCYC 2 cut(s) 408, 619
ErhI CCWWGG 1 cut(s) 596
Esp3I CGTCTC 1 cut(s) 672
FaeI CATG 6 cut(s) 10, 19, 205, 268, 339, 441
FatI CATG 6 cut(s) 6, 15, 201, 264, 335, 437
FauI CCCGC 1 cut(s) 358
FbaI TGATCA 1 cut(s) 9
Fnu4HI GCNGC 3 cut(s) 292, 480, 733
FriOI GRGCYC 2 cut(s) 408, 619
Fsp4HI GCNGC 3 cut(s) 292, 480, 733
FspBI CTAG 3 cut(s) 278, 590, 597
FspI TGCGCA 1 cut(s) 316
GlaI GCGC 3 cut(s) 65, 316, 731
GluI GCNGC 3 cut(s) 292, 480, 733
GsuI CTGGAG 2 cut(s) 132, 367
HaeIII GGCC 4 cut(s) 601, 735, 763, 780
HhaI GCGC 3 cut(s) 66, 317, 732
Hin1II CATG 6 cut(s) 10, 19, 205, 268, 339, 441
Hin6I GCGC 3 cut(s) 64, 315, 730
HinP1I GCGC 3 cut(s) 64, 315, 730
HinfI GANTC 2 cut(s) 116, 524
HphI GGTGA 3 cut(s) 230, 668, 769
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 3 cut(s) 100, 149, 620
Hpy99I CGWCG 1 cut(s) 190
HpyAV CCTTC 2 cut(s) 205, 740
HpyCH4III ACNGT 1 cut(s) 574
HpyCH4IV ACGT 2 cut(s) 412, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 398, 777
HpyF3I CTNAG 3 cut(s) 402, 450, 726
HpySE526I ACGT 2 cut(s) 412, 564
Hsp92II CATG 6 cut(s) 10, 19, 205, 268, 339, 441
HspAI GCGC 3 cut(s) 64, 315, 730
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 4 cut(s) 9, 77, 214, 585
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 3 cut(s) 42, 58, 586
MaeI CTAG 3 cut(s) 278, 590, 597
MaeII ACGT 2 cut(s) 412, 564
MaeIII GTNAC 5 cut(s) 236, 260, 560, 568, 719
MalI GATC 4 cut(s) 11, 79, 216, 587
MboI GATC 4 cut(s) 9, 77, 214, 585
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 2 cut(s) 408, 619
MluCI AATT 2 cut(s) 330, 659
Mph1103I ATGCAT 1 cut(s) 443
MslI CAYNNNNRTG 1 cut(s) 513
MspR9I CCNGG 3 cut(s) 385, 465, 783
MunI CAATTG 1 cut(s) 330
MvaI CCWGG 3 cut(s) 385, 465, 783
MvnI CGCG 5 cut(s) 64, 66, 541, 613, 732
MwoI GCNNNNNNNGC 3 cut(s) 72, 398, 777
NdeII GATC 4 cut(s) 9, 77, 214, 585
NlaIII CATG 6 cut(s) 10, 19, 205, 268, 339, 441
NmuCI GTSAC 3 cut(s) 236, 560, 568
NsbI TGCGCA 1 cut(s) 316
NsiI ATGCAT 1 cut(s) 443
NspI RCATGY 2 cut(s) 19, 441
PaeI GCATGC 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 618
PasI CCCWGGG 1 cut(s) 782
PceI AGGCCT 2 cut(s) 601, 763
PfeI GAWTC 2 cut(s) 116, 524
PkrI GCNGC 3 cut(s) 293, 481, 734
PsiI TTATAA 1 cut(s) 156
Psp124BI GAGCTC 2 cut(s) 408, 619
Psp6I CCWGG 3 cut(s) 383, 463, 781
PspGI CCWGG 3 cut(s) 383, 463, 781
PspPI GGNCC 1 cut(s) 779
PstI CTGCAG 1 cut(s) 77
PsuI RGATCY 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 513
SacI GAGCTC 2 cut(s) 408, 619
SatI GCNGC 3 cut(s) 292, 480, 733
Sau3AI GATC 4 cut(s) 9, 77, 214, 585
Sau96I GGNCC 1 cut(s) 779
ScrFI CCNGG 3 cut(s) 385, 465, 783
SduI GDGCHC 2 cut(s) 408, 619
SfaNI GCATC 3 cut(s) 42, 58, 586
SfcI CTRYAG 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 618
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 3 cut(s) 100, 147, 618
SmoI CTYRAG 3 cut(s) 100, 147, 618
SphI GCATGC 1 cut(s) 441
Sse9I AATT 2 cut(s) 330, 659
SseBI AGGCCT 2 cut(s) 601, 763
SsiI CCGC 6 cut(s) 62, 291, 351, 605, 611, 732
SspMI CTAG 3 cut(s) 278, 590, 597
SstI GAGCTC 2 cut(s) 408, 619
StuI AGGCCT 2 cut(s) 601, 763
StyD4I CCNGG 3 cut(s) 383, 463, 781
StyI CCWWGG 1 cut(s) 596
TaaI ACNGT 1 cut(s) 574
TaiI ACGT 2 cut(s) 415, 567
TaqI TCGA 1 cut(s) 619
TasI AATT 2 cut(s) 330, 659
TauI GCSGC 2 cut(s) 294, 735
TfiI GAWTC 2 cut(s) 116, 524
TscAI CASTG 1 cut(s) 709
TseFI GTSAC 3 cut(s) 236, 560, 568
TseI GCWGC 1 cut(s) 479
Tsp45I GTSAC 3 cut(s) 236, 560, 568
TspDTI ATGAA 1 cut(s) 655
TspRI CASTG 1 cut(s) 709
XapI RAATTY 1 cut(s) 659
XceI RCATGY 2 cut(s) 19, 441
XhoI CTCGAG 1 cut(s) 618
XmaJI CCTAGG 1 cut(s) 596
XspI CTAG 3 cut(s) 278, 590, 597
Zsp2I ATGCAT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.