RchiOBHm_Chr1g0384471

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001157
Physical Location & Seq
Reverse (-)
68299819 .. 68303400
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3288 bp
ATGGGATCAATTGATAATAAACCCATTAAAGTATTCATCGATTGCGGGTCCGCGTGCAACTTCCTCCATCCTTCGATTGCTCACCAACTCCAACTGAAGGTCTCCAATGCAGTGGGCATCCAATTCCACTCAGCCTCTGGGGAACCACTGCGTCCTTCTGGCATGGTTCACGAGGTGAAGATTTCTATCCAAGATTATTCCTTCACGAGCTCTTTCCTTCTCTTACCTGTTACAGGATGTGATCTGGTGTTGGGGGCTCCATGGCTCGACACGTTGGGTTTAATAGCGTGGCACTTTCAAGAAAAAATAATGGGTTTTTTTACGAATGGGCAATTCCATGTGCTAAAAGGCCTCACCACCACTCACCAGTCAACCCACACTCAAGAACTCTTTGCCCTCATTCCATCTACTCATTGGGACTCCACGGCCCAAACTCTGATCCCAACTAACCCACCTCAATCTGAGCCCCACACTCCCCCAATTCAAGCCTTACTTGACTCATTCCCAGATATATTTTCCCAACCCGTAAGTTTACCACCAGCTAGGCCAATTGATCATCGCATCACCCTCATTCCTAATACCAACCCAATCAATGTCCGCCCTTATCGTTACCCCCATTCTCAAAAGGCTGAGCTTGAGAAACAAGTAGCCGAAATGTTGTCCAGTGGATTGATAAGGCCTAGCTCTAGCCCATTTTCTTCTCCGGTCCTGTTGGTTAAAAAGAAGGAGGGCACATGGAGATTTTGTGTCGACTACAGCTCTTTAAATGCGGCAACGGTCAAGGATCGCTTTCCCATTCCTGTCGTCGACGAATTATTGGATGAGTTACATGGTGCCACTTGTTTTACTAAACTCGACCTAAGATCGGGCTACCATCAAGTGAGGATGGCAGAGGAAGACATCCCTAAAACAGCTTTTAGAACTCATGAAGGTCACTTTGAGTTTGTTGTAATGCCATTCGGCCTTACGAACGCACCATCCACTTTCCAAGCCTTAATGAATCAAGTCTTCAAGCCATTCTTACGCAAGAGTGTGTTGGTATTTTTCGATGACATCTTGGTATATAGCAAAGACTTGCAATCTCATGTGGTGCATCTTAGAGAAGTTTTTGCCTTGCTTCGGTCCAATCTGCTAAGGCTCAAAATGTCCAAGTGTAGAATTGCTACAGCCCAAGTGGATTATTTAGGGCATGTCATTTCATCTTTGGGTGTTGCCGTTGACCCAACAAAAGTCCAAGCCATACAGGCCTGGCCCAAACCCACAACCCTCAAAGGCCTGCGCGGCTTCCTCGGGATCACCGGCTACTATCGCCGCTTCATTCACAATTACGGCGTCATAGCCAAACCTCTTACTGAGATGCTCAAGACAGAAAATTTCATATGGACTCCCGCTTCTCACTCAGCGTTTGACGAATTGAAGCATGCTCTAATGACAGCTCCGGTCTTAGCCTTGCCTGATTTCTCTATCCCATTCATTGTCGAAACAGATGCTAGCGGTTTGGGTATAGGAGCTGTCCTTTCTCAACGCAAACATCCGATTGCATTCTTGAGTAAGCCTCTATCTCCTCGCAATCAAGTGCTATCTGTTTATGATAAAGAAATGCTTGCAGTCATGCATGCCATAGATAAATGGCGCCCTTACCTCTTGGGAAATCCGTTCACCATCATCACTGATCACCAAACACTCAAGCATCTCCTGGACCAGCGAATTTCAACCCCTTCGCAGCATAAATGGCTTGCTAAGCTCATGGGCTATCAGTACACAATTGAATACCGACTGGGAAAACTAAATACGGTACCCGATTTACTGTCTCGACGCCATGAAATGTGCTCAATTCAAACCATCTCAGCTCCAGTGTTCGACGGTTTAGCTCACATCACTAATGCATGTCTTCGAGATACGGAAGCGCAGACCATCATCTCTCAGTTACAACAAGGCCTTCCCAGCAAAAAGGGGTTTACTTTGCATAACACACATCTGTTATACAAAGGCAAGTTCTTTGTTCCGTTATCCTCGACTTGGCGTACTAAGTTGTTATATGAGTTTCACTCATCTCTCCAAGCGGGTCATTCGGGTTTTTTACGCACTTACCATCGCCTTTCTCGCAATTTTGCATGGCCGGGTATGCGCAAAGAAATCAAACTGTTCATATCAGAATGTGATCAATGTCAAAGGCAAAAGTACGAGACCATCAATCCTCCAGGTATGCTGCAGCCCCTGCCCATCCCTGATAATACTTGGCTAGACATTTCAATGGATTTCGTGGATGGATTACCACTGTCTCAAGGTCACAATGCCGTGTTGGTTGTTGTAGACCGGTTATCCAAATATGGTCACTTTATTCCGGTGTCTCATCCCTATACAGCCACTCACATTGCAGAAGCTTTCATGAAAGAAGTGTTCAGACTTCACGGCATGCCGAAGTCAATTGTAAGTGATCGAGACCCCATATTCATTAGCCATTTTTGGGAGGCATTCTTCAAATTGCAAGGCACCACATTATGCCGCAGCTCAGCCTATCATCCCCAAACGGATGGCCAAACCGAGGTAGTAAATCGTTCTCTCGAACACTACTTACGATGTTTCGCAACCGATAAGCCGGCTTCTTGGAGTTCATTATTACATTGGGCAGAATGGTGGTATAACTCCACCTACCACTCTACCATAAAGATGACCCCATTTCAAGCTGTGTACGGCACCCCTCCACCAGCAATTTCATCCTACCTTCCCGGGTCTACCTCCGTCAACGCTGTGGACTGTGCTCTAAAGAATCGGGATGAGCTGCTCCAATTGTTGAAAGTCCACATGAATCAGGCTCAGAACCGTATGAAGCAGCAAGCGGACCGCCACCGTACTGAAAGAGAATTCGCTGCCGGTGACTGGGTATTCTTGAAGCTCCATCCTTATAGACAGCGTTCATTACTCAAACATCCTTCCCATAAACTCTCTCCCAGGTTTTATGGTCCTTTTCTCATTCAAAAAAGAGTTGGAAAGGCTGCTTATCGCCTGTCACTTCCCACCTCTTGCCGCCTTCATCCGGTTTTTCATGTTTCCCTTCTCAAAAAGAGAATTGGAGGAGGAACTCCTCTCACAACCACACTGCCAGAATTTGATGATCAAGGGGAGCTGTTATGGCGTCCGGTGCGAGTGTTAGATATGGCTGTTATTAAGAAAAAGAAACGCAACACCACGCAATGGCTCATTCAATGGCATGGCCTACCGACTGCAGATGCTACTTGGGAGGATGCACACACCATCAAGGCACGCTTCCCTACATTTGGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve

Protein Analysis

1095

Amino Acids

124.21

Weight (kDa)

9.36

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVP_2 PF08284 4 - 90 1e-05 Retroviral aspartyl protease
RVT_1 PF00078 239 - 399 7.7e-38 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 461 - 555 3.4e-31 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 486 - 585 1.4e-30 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 673 - 728 1.4e-17 Integrase zinc binding domain
rve PF00665 751 - 842 8.7e-07 Integrase core domain
SH3_Tf2-1 PF24626 959 - 1022 2.9e-22 Tf2-1-like, SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2129
Acc65I GGTACC 1 cut(s) 1795
AccB1I GGYRCC 6 cut(s) 833, 1632, 1795, 2492, 2696, 3281
AccB7I CCANNNNNTGG 1 cut(s) 3195
AccI GTMKAC 4 cut(s) 750, 807, 2313, 2735
AccII CGCG 2 cut(s) 53, 1281
AclWI GGATC 4 cut(s) 13, 433, 792, 1301
AcoI YGGCCR 2 cut(s) 2117, 2536
AcsI RAATTY 4 cut(s) 1372, 1707, 2864, 3107
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 4 cut(s) 1332, 1633, 1816, 3136
AdeI CACNNNGTG 1 cut(s) 175
AfaI GTAC 6 cut(s) 1760, 1797, 2026, 2183, 2693, 2854
AflIII ACRYGT 1 cut(s) 270
AgeI ACCGGT 1 cut(s) 2316
AjnI CCWGG 4 cut(s) 1247, 1695, 2200, 2951
AloI GAACNNNNNNTCC 2 cut(s) 962, 994
Alw21I GWGCWC 3 cut(s) 212, 1832, 2764
Alw26I GTCTC 6 cut(s) 106, 1815, 2180, 2286, 2355, 2436
AlwI GGATC 4 cut(s) 13, 433, 792, 1301
AlwNI CAGNNNCTG 2 cut(s) 137, 2218
Ama87I CYCGRG 2 cut(s) 1289, 2729
ApeKI GCWGC 8 cut(s) 1723, 2209, 2212, 2508, 2782, 2833, 2870, 2996
ApoI RAATTY 4 cut(s) 1372, 1707, 2864, 3107
AsiGI ACCGGT 1 cut(s) 2316
Asp718I GGTACC 1 cut(s) 1795
AspLEI GCGC 4 cut(s) 1281, 1635, 1909, 2130
AspS9I GGNCC 8 cut(s) 48, 427, 706, 1122, 1251, 1699, 2842, 2963
AsuC2I CCSGG 3 cut(s) 2121, 2730, 2731
AsuHPI GGTGA 9 cut(s) 74, 187, 346, 356, 556, 1288, 1651, 1667, 2888
AsuNHI GCTAGC 1 cut(s) 1490
AvaI CYCGRG 2 cut(s) 1289, 2729
AvaII GGWCC 6 cut(s) 48, 706, 1122, 1699, 2842, 2963
BaeGI GKGCMC 1 cut(s) 734
BalI TGGCCA 1 cut(s) 2538
BanI GGYRCC 6 cut(s) 833, 1632, 1795, 2492, 2696, 3281
BanII GRGCYC 3 cut(s) 212, 259, 468
BauI CACGAG 2 cut(s) 170, 205
BbsI GAAGAC 3 cut(s) 903, 1000, 1883
Bbv12I GWGCWC 3 cut(s) 212, 1832, 2764
BbvI GCAGC 8 cut(s) 1735, 2196, 2224, 2520, 2769, 2845, 2857, 2983
BceAI ACGGC 6 cut(s) 441, 1199, 1345, 2282, 2428, 2710
BcgI CGANNNNNNTGC 2 cut(s) 1469, 1503
BciT130I CCWGG 4 cut(s) 1249, 1697, 2202, 2953
BclI TGATCA 4 cut(s) 553, 1672, 2161, 3115
BcnI CCSGG 3 cut(s) 2121, 2730, 2731
BcoDI GTCTC 6 cut(s) 106, 1815, 2180, 2286, 2355, 2436
BfaI CTAG 5 cut(s) 543, 681, 687, 1491, 2243
BfmI CTRYAG 4 cut(s) 754, 1164, 2210, 3225
BfoI RGCGCY 1 cut(s) 1636
BglI GCCNNNNNGGC 2 cut(s) 1244, 1281
BlpI GCTNAGC 3 cut(s) 630, 1740, 2512
Bme1390I CCNGG 7 cut(s) 1249, 1697, 2121, 2202, 2730, 2731, 2953
Bme18I GGWCC 6 cut(s) 48, 706, 1122, 1699, 2842, 2963
BmeT110I CYCGRG 2 cut(s) 1289, 2729
BmgT120I GGNCC 8 cut(s) 48, 427, 706, 1122, 1251, 1699, 2842, 2963
BmiI GGNNCC 9 cut(s) 49, 144, 258, 835, 1634, 1797, 2494, 2698, 3283
BmrFI CCNGG 7 cut(s) 1249, 1697, 2121, 2202, 2730, 2731, 2953
BmrI ACTGGG 2 cut(s) 1787, 2890
BmsI GCATC 8 cut(s) 126, 570, 1102, 1347, 1477, 1699, 3220, 3235
BmtI GCTAGC 1 cut(s) 1494
BmuI ACTGGG 2 cut(s) 1787, 2890
BpiI GAAGAC 3 cut(s) 903, 1000, 1883
BplI GAGNNNNNCTC 4 cut(s) 1540, 1572, 2033, 2065
BpmI CTGGAG 2 cut(s) 1836, 2184
Bpu10I CCTNAGC 1 cut(s) 1133
Bpu1102I GCTNAGC 3 cut(s) 630, 1740, 2512
BpuEI CTTGAG 6 cut(s) 366, 656, 1346, 1567, 1670, 2268
BpuMI CCSGG 3 cut(s) 2121, 2730, 2731
Bsa29I ATCGAT 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 185
BsaHI GRCGYC 4 cut(s) 1332, 1633, 1816, 3136
BsaI GGTCTC 3 cut(s) 106, 2180, 2436
BsaJI CCNNGG 6 cut(s) 260, 423, 1288, 2544, 2729, 2951
BsaWI WCCGGW 6 cut(s) 703, 1438, 2316, 2344, 3037, 3139
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bse118I RCCGGY 4 cut(s) 1298, 2316, 2599, 2873
Bse1I ACTGG 5 cut(s) 367, 663, 1782, 1853, 2885
Bse3DI GCAATG 2 cut(s) 2373, 3200
Bse8I GATNNNNATC 1 cut(s) 185
BseBI CCWGG 4 cut(s) 1249, 1697, 2202, 2953
BseCI ATCGAT 1 cut(s) 39
BseDI CCNNGG 6 cut(s) 260, 423, 1288, 2544, 2729, 2951
BseJI GATNNNNATC 1 cut(s) 185
BseMI GCAATG 2 cut(s) 2373, 3200
BseMII CTCAG 9 cut(s) 144, 453, 621, 1344, 1413, 1860, 1937, 2526, 2831
BseNI ACTGG 5 cut(s) 367, 663, 1782, 1853, 2885
BseRI GAGGAG 3 cut(s) 1554, 3075, 3090
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 8 cut(s) 1735, 2196, 2224, 2520, 2769, 2845, 2857, 2983
BseYI CCCAGC 1 cut(s) 1943
Bsh1236I CGCG 2 cut(s) 53, 1281
BshNI GGYRCC 6 cut(s) 833, 1632, 1795, 2492, 2696, 3281
BshTI ACCGGT 1 cut(s) 2316
BshVI ATCGAT 1 cut(s) 39
BsiHKAI GWGCWC 3 cut(s) 212, 1832, 2764
BsiHKCI CYCGRG 2 cut(s) 1289, 2729
BslFI GGGAC 1 cut(s) 431
BsmAI GTCTC 6 cut(s) 106, 1815, 2180, 2286, 2355, 2436
BsmFI GGGAC 1 cut(s) 431
BsmI GAATGC 2 cut(s) 1541, 2474
Bso31I GGTCTC 3 cut(s) 106, 2180, 2436
BsoBI CYCGRG 2 cut(s) 1289, 2729
Bsp1286I GDGCHC 6 cut(s) 212, 259, 468, 734, 1832, 2764
Bsp1720I GCTNAGC 3 cut(s) 630, 1740, 2512
Bsp19I CCATGG 1 cut(s) 260
BspCNI CTCAG 9 cut(s) 143, 454, 622, 1345, 1412, 1859, 1936, 2525, 2830
BspDI ATCGAT 1 cut(s) 39
BspFNI CGCG 2 cut(s) 53, 1281
BspHI TCATGA 2 cut(s) 925, 2388
BspLI GGNNCC 9 cut(s) 49, 144, 258, 835, 1634, 1797, 2494, 2698, 3283
BspMAI CTGCAG 2 cut(s) 2214, 3229
BspOI GCTAGC 1 cut(s) 1494
BspPI GGATC 4 cut(s) 13, 433, 792, 1301
BspT107I GGYRCC 6 cut(s) 833, 1632, 1795, 2492, 2696, 3281
BspTNI GGTCTC 3 cut(s) 106, 2180, 2436
BsrDI GCAATG 2 cut(s) 2373, 3200
BsrFI RCCGGY 4 cut(s) 1298, 2316, 2599, 2873
BsrI ACTGG 5 cut(s) 367, 663, 1782, 1853, 2885
BssAI RCCGGY 4 cut(s) 1298, 2316, 2599, 2873
BssECI CCNNGG 6 cut(s) 260, 423, 1288, 2544, 2729, 2951
BssNI GRCGYC 4 cut(s) 1332, 1633, 1816, 3136
BssSI CACGAG 2 cut(s) 170, 205
BssT1I CCWWGG 1 cut(s) 260
Bst2BI CACGAG 2 cut(s) 170, 205
Bst2UI CCWGG 4 cut(s) 1249, 1697, 2202, 2953
Bst4CI ACNGT 9 cut(s) 778, 1795, 1809, 1865, 2145, 2280, 2759, 2825, 2852
BstACI GRCGYC 4 cut(s) 1332, 1633, 1816, 3136
BstAPI GCANNNNNTGC 1 cut(s) 2218
BstDSI CCRYGG 2 cut(s) 260, 423
BstENI CCTNNNNNAGG 1 cut(s) 231
BstFNI CGCG 2 cut(s) 53, 1281
BstH2I RGCGCY 1 cut(s) 1636
BstHHI GCGC 4 cut(s) 1281, 1635, 1909, 2130
BstMAI GTCTC 6 cut(s) 106, 1815, 2180, 2286, 2355, 2436
BstNI CCWGG 4 cut(s) 1249, 1697, 2202, 2953
BstNSI RCATGY 5 cut(s) 1193, 1424, 1619, 1890, 2419
BstSCI CCNGG 7 cut(s) 1247, 1695, 2119, 2200, 2728, 2729, 2951
BstSFI CTRYAG 4 cut(s) 754, 1164, 2210, 3225
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 2 cut(s) 53, 1281
BstV1I GCAGC 8 cut(s) 1735, 2196, 2224, 2520, 2769, 2845, 2857, 2983
BstV2I GAAGAC 3 cut(s) 903, 1000, 1883
BstXI CCANNNNNNTGG 2 cut(s) 112, 2534
Bsu15I ATCGAT 1 cut(s) 39
BsuTUI ATCGAT 1 cut(s) 39
BtgI CCRYGG 2 cut(s) 260, 423
BtgZI GCGATG 2 cut(s) 542, 2078
BtsI GCAGTG 3 cut(s) 117, 146, 3098
BtsIMutI CAGTG 7 cut(s) 117, 146, 670, 1668, 1860, 2276, 3098
CaiI CAGNNNCTG 2 cut(s) 137, 2218
CciI TCATGA 2 cut(s) 925, 2388
CfoI GCGC 4 cut(s) 1281, 1635, 1909, 2130
Cfr10I RCCGGY 4 cut(s) 1298, 2316, 2599, 2873
Cfr13I GGNCC 8 cut(s) 48, 427, 706, 1122, 1251, 1699, 2842, 2963
Cfr9I CCCGGG 1 cut(s) 2729
ClaI ATCGAT 1 cut(s) 39
CpoI CGGWCCG 1 cut(s) 2842
CseI GACGC 4 cut(s) 140, 1321, 1824, 3125
Csp6I GTAC 6 cut(s) 1759, 1796, 2025, 2182, 2692, 2853
CspAI ACCGGT 1 cut(s) 2316
CspCI CAANNNNNGTGG 2 cut(s) 2356, 2391
CspI CGGWCCG 1 cut(s) 2842
CviQI GTAC 6 cut(s) 1759, 1796, 2025, 2182, 2692, 2853
DinI GGCGCC 1 cut(s) 1634
DraI TTTAAA 1 cut(s) 765
DraIII CACNNNGTG 1 cut(s) 175
EaeI YGGCCR 2 cut(s) 2117, 2536
EciI GGCGGA 1 cut(s) 587
Ecl136II GAGCTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 260
Eco147I AGGCCT 5 cut(s) 351, 679, 1247, 1275, 1938
Eco24I GRGCYC 3 cut(s) 212, 259, 468
Eco31I GGTCTC 3 cut(s) 106, 2180, 2436
Eco47I GGWCC 6 cut(s) 48, 706, 1122, 1699, 2842, 2963
Eco53kI GAGCTC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 116
Eco88I CYCGRG 2 cut(s) 1289, 2729
EcoICRI GAGCTC 1 cut(s) 210
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoRI GAATTC 1 cut(s) 2864
EcoRII CCWGG 4 cut(s) 1247, 1695, 2200, 2951
EcoT14I CCWWGG 1 cut(s) 260
EcoT22I ATGCAT 2 cut(s) 1617, 1888
EcoT38I GRGCYC 3 cut(s) 212, 259, 468
EgeI GGCGCC 1 cut(s) 1634
EheI GGCGCC 1 cut(s) 1634
ErhI CCWWGG 1 cut(s) 260
FaqI GGGAC 1 cut(s) 431
FauI CCCGC 3 cut(s) 38, 1396, 2056
FauNDI CATATG 1 cut(s) 1379
FbaI TGATCA 4 cut(s) 553, 1672, 2161, 3115
FblI GTMKAC 4 cut(s) 750, 807, 2313, 2735
FriOI GRGCYC 3 cut(s) 212, 259, 468
FspBI CTAG 5 cut(s) 543, 681, 687, 1491, 2243
FspI TGCGCA 1 cut(s) 2129
GlaI GCGC 4 cut(s) 1280, 1634, 1908, 2129
GsaI CCCAGC 1 cut(s) 1947
GsuI CTGGAG 2 cut(s) 1836, 2184
HaeII RGCGCY 1 cut(s) 1636
HgaI GACGC 4 cut(s) 140, 1321, 1824, 3125
HhaI GCGC 4 cut(s) 1281, 1635, 1909, 2130
Hin1I GRCGYC 4 cut(s) 1332, 1633, 1816, 3136
Hin6I GCGC 4 cut(s) 1279, 1633, 1907, 2128
HinP1I GCGC 4 cut(s) 1279, 1633, 1907, 2128
HincII GTYRAC 5 cut(s) 372, 751, 808, 1219, 2746
HindII GTYRAC 5 cut(s) 372, 751, 808, 1219, 2746
HindIII AAGCTT 1 cut(s) 2382
HinfI GANTC 6 cut(s) 419, 497, 1000, 1384, 2770, 2809
HphI GGTGA 9 cut(s) 74, 187, 346, 356, 556, 1288, 1651, 1667, 2888
Hpy188I TCNGA 6 cut(s) 438, 463, 1536, 2155, 2405, 2820
Hpy99I CGWCG 4 cut(s) 809, 812, 1818, 1865
HpyCH4III ACNGT 9 cut(s) 778, 1795, 1809, 1865, 2145, 2280, 2759, 2825, 2852
HpyCH4IV ACGT 1 cut(s) 272
HpySE526I ACGT 1 cut(s) 272
Hsp92I GRCGYC 4 cut(s) 1332, 1633, 1816, 3136
HspAI GCGC 4 cut(s) 1279, 1633, 1907, 2128
KasI GGCGCC 1 cut(s) 1632
KpnI GGTACC 1 cut(s) 1799
KroI GCCGGC 1 cut(s) 2599
KroNI GCCGGC 1 cut(s) 2601
Ksp22I TGATCA 4 cut(s) 553, 1672, 2161, 3115
LmnI GCTCC 7 cut(s) 262, 1441, 1508, 1855, 2790, 2901, 3124
Lsp1109I GCAGC 8 cut(s) 1735, 2196, 2224, 2520, 2769, 2845, 2857, 2983
LweI GCATC 8 cut(s) 126, 570, 1102, 1347, 1477, 1699, 3220, 3235
MaeI CTAG 5 cut(s) 543, 681, 687, 1491, 2243
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 9 cut(s) 229, 608, 825, 932, 1926, 2288, 2333, 2876, 3009
MboII GAAGA 6 cut(s) 190, 690, 908, 1000, 1883, 2470
MfeI CAATTG 5 cut(s) 9, 549, 1764, 2427, 2789
MhlI GDGCHC 6 cut(s) 212, 259, 468, 734, 1832, 2764
MlsI TGGCCA 1 cut(s) 2538
MluNI TGGCCA 1 cut(s) 2538
Mly113I GGCGCC 1 cut(s) 1633
MlyI GAGTC 3 cut(s) 413, 491, 1378
MmeI TCCRAC 2 cut(s) 115, 2968
Mox20I TGGCCA 1 cut(s) 2538
Mph1103I ATGCAT 2 cut(s) 1617, 1888
MroNI GCCGGC 1 cut(s) 2599
MscI TGGCCA 1 cut(s) 2538
MseI TTAA 6 cut(s) 27, 281, 717, 764, 995, 3168
MslI CAYNNNNRTG 2 cut(s) 2252, 2669
Msp20I TGGCCA 1 cut(s) 2538
MspR9I CCNGG 7 cut(s) 1249, 1697, 2121, 2202, 2730, 2731, 2953
MunI CAATTG 5 cut(s) 9, 549, 1764, 2427, 2789
Mva1269I GAATGC 2 cut(s) 1541, 2474
MvaI CCWGG 4 cut(s) 1249, 1697, 2202, 2953
MvnI CGCG 2 cut(s) 53, 1281
NaeI GCCGGC 1 cut(s) 2601
NarI GGCGCC 1 cut(s) 1633
NciI CCSGG 3 cut(s) 2121, 2730, 2731
NcoI CCATGG 1 cut(s) 260
NdeI CATATG 1 cut(s) 1379
NgoMIV GCCGGC 1 cut(s) 2599
NheI GCTAGC 1 cut(s) 1490
NlaIV GGNNCC 9 cut(s) 49, 144, 258, 835, 1634, 1797, 2494, 2698, 3283
NmuCI GTSAC 5 cut(s) 932, 2288, 2333, 2876, 3009
NsbI TGCGCA 1 cut(s) 2129
NsiI ATGCAT 2 cut(s) 1617, 1888
NspI RCATGY 5 cut(s) 1193, 1424, 1619, 1890, 2419
PaeI GCATGC 3 cut(s) 1424, 1619, 2419
PagI TCATGA 2 cut(s) 925, 2388
PceI AGGCCT 5 cut(s) 351, 679, 1247, 1275, 1938
PctI GAATGC 2 cut(s) 1541, 2474
PdiI GCCGGC 1 cut(s) 2601
PfeI GAWTC 3 cut(s) 1000, 2770, 2809
PflMI CCANNNNNTGG 1 cut(s) 3195
PfoI TCCNGGA 1 cut(s) 1695
PinAI ACCGGT 1 cut(s) 2316
PleI GAGTC 3 cut(s) 413, 491, 1378
PluTI GGCGCC 1 cut(s) 1636
PpsI GAGTC 3 cut(s) 413, 491, 1378
Psp124BI GAGCTC 1 cut(s) 212
Psp6I CCWGG 4 cut(s) 1247, 1695, 2200, 2951
PspFI CCCAGC 1 cut(s) 1943
PspGI CCWGG 4 cut(s) 1247, 1695, 2200, 2951
PspN4I GGNNCC 9 cut(s) 49, 144, 258, 835, 1634, 1797, 2494, 2698, 3283
PspPI GGNCC 8 cut(s) 48, 427, 706, 1122, 1251, 1699, 2842, 2963
PsrI GAACNNNNNNTAC 2 cut(s) 2559, 2591
PstI CTGCAG 2 cut(s) 2214, 3229
PstNI CAGNNNCTG 2 cut(s) 137, 2218
RsaI GTAC 6 cut(s) 1760, 1797, 2026, 2183, 2693, 2854
RsaNI GTAC 6 cut(s) 1759, 1796, 2025, 2182, 2692, 2853
RseI CAYNNNNRTG 2 cut(s) 2252, 2669
Rsr2I CGGWCCG 1 cut(s) 2842
RsrII CGGWCCG 1 cut(s) 2842
SacI GAGCTC 1 cut(s) 212
SalI GTCGAC 2 cut(s) 749, 806
SaqAI TTAA 6 cut(s) 27, 281, 717, 764, 995, 3168
Sau96I GGNCC 8 cut(s) 48, 427, 706, 1122, 1251, 1699, 2842, 2963
SchI GAGTC 3 cut(s) 413, 491, 1378
ScrFI CCNGG 7 cut(s) 1249, 1697, 2121, 2202, 2730, 2731, 2953
SduI GDGCHC 6 cut(s) 212, 259, 468, 734, 1832, 2764
SfaNI GCATC 8 cut(s) 126, 570, 1102, 1347, 1477, 1699, 3220, 3235
SfcI CTRYAG 4 cut(s) 754, 1164, 2210, 3225
SfoI GGCGCC 1 cut(s) 1634
SgrDI CGTCGACG 1 cut(s) 806
SinI GGWCC 6 cut(s) 48, 706, 1122, 1699, 2842, 2963
SmaI CCCGGG 1 cut(s) 2731
SmiMI CAYNNNNRTG 2 cut(s) 2252, 2669
SmlI CTYRAG 6 cut(s) 381, 635, 1361, 1546, 1685, 2283
SmoI CTYRAG 6 cut(s) 381, 635, 1361, 1546, 1685, 2283
SphI GCATGC 3 cut(s) 1424, 1619, 2419
SseBI AGGCCT 5 cut(s) 351, 679, 1247, 1275, 1938
SspDI GGCGCC 1 cut(s) 1632
SspMI CTAG 5 cut(s) 543, 681, 687, 1491, 2243
SstI GAGCTC 1 cut(s) 212
StuI AGGCCT 5 cut(s) 351, 679, 1247, 1275, 1938
StyD4I CCNGG 7 cut(s) 1247, 1695, 2119, 2200, 2728, 2729, 2951
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 9 cut(s) 778, 1795, 1809, 1865, 2145, 2280, 2759, 2825, 2852
TaiI ACGT 1 cut(s) 275
TaqII GACCGA 1 cut(s) 1110
TatI WGTACW 1 cut(s) 1758
TauI GCSGC 5 cut(s) 773, 1284, 1314, 2508, 3030
TfiI GAWTC 3 cut(s) 1000, 2770, 2809
Tru1I TTAA 6 cut(s) 27, 281, 717, 764, 995, 3168
Tru9I TTAA 6 cut(s) 27, 281, 717, 764, 995, 3168
TscAI CASTG 7 cut(s) 117, 153, 670, 1675, 1860, 2283, 3105
TseFI GTSAC 5 cut(s) 932, 2288, 2333, 2876, 3009
TseI GCWGC 8 cut(s) 1723, 2209, 2212, 2508, 2782, 2833, 2870, 2996
Tsp45I GTSAC 5 cut(s) 932, 2288, 2333, 2876, 3009
TspGWI ACGGA 5 cut(s) 1644, 1916, 1995, 2546, 2731
TspMI CCCGGG 1 cut(s) 2729
TspRI CASTG 7 cut(s) 117, 153, 670, 1675, 1860, 2283, 3105
Van91I CCANNNNNTGG 1 cut(s) 3195
VpaK11BI GGWCC 6 cut(s) 48, 706, 1122, 1699, 2842, 2963
XagI CCTNNNNNAGG 1 cut(s) 231
XapI RAATTY 4 cut(s) 1372, 1707, 2864, 3107
XceI RCATGY 5 cut(s) 1193, 1424, 1619, 1890, 2419
XcmI CCANNNNNNNNNTGG 2 cut(s) 134, 411
XmaI CCCGGG 1 cut(s) 2729
XmiI GTMKAC 4 cut(s) 750, 807, 2313, 2735
XspI CTAG 5 cut(s) 543, 681, 687, 1491, 2243
Zsp2I ATGCAT 2 cut(s) 1617, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.