Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000802

Physical Location & Seq

Genomic Coordinates Forward (+)

10206602 .. 10211962

Gene Structure 5361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29524

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4899 bp

ATGCCGAGGCCAAATGTTTCTCGTCTAGGTAAGAGAGACTCCGGCGACGTTGATGACTCCCTCCGTGACTCTAAACGGCCTCGTCCGGACCTCCCTGAGATACTTGTACGAGTAGCTGACATGCCCAGGTGGAGTCGAAAAGCCTTTGACGGGATCCATGAGCTGAACAGGGTGGATAGTATTGTTTATGATACTGCCCTGTTTGAGACCGACAACATCCTCCTGAGTGCTCCAACTGGACCCGGGGAAGGTAATGTTGCCATCCTCACCATTCTCCAGCAGTTCAGTATAAATATGGATAGATGTGATGATTATAGTGTAAATCACAGAGGGTACAAAGTATTGTATTTGGCTTCTGCTACAGCTATTGTTCGTAAGGTGGTGGCACATTTGAGTGCAAGATTGAGGTCTTTTGGTGCCAAGGTGAAAGAAATGAGTGGTCATGACCAGACCCTCACTCACTCACAGATTGAAGAGACTCAGATTATTGTCACAACTCCTAAGGATTGGGAAATGATCACAAGAAAGTTTGGAGAACACACTTGTACACTGGTCATTATTGATGGAGTTCACCTTCTCCACTCTACTAGAGGCCCCGTTCTTGAAAGCATTGTTGCTAGAACGGTTAGGATGCTGGAGACCACCAAGAAACATACCCGATTGGTGGGTTTATCTGCTGTGTTCCCCAACTATGAAGACGTGGCGCGATTCTTGCGTGTTGATGATAATGGTCTTTTTAATTTTGACAATAGTTGCAGACCTGTTCCTCTTTCTCTCAAGTGTGTTGGAATGGAGGATAGAAACCCACTGAGTAGGTTTCAATTGATGAATCAACGCTGTTATGAAAACGTGATAGCTGTGCCAGGAAATCATCCTGTTCTTATTTTTGTCCACTCAGAGAACCAGACAGTCGAAACAGCTCGCTATATAAGGGATACCGCAGTTGCTAATGGTACCATTGATGCATTTAAGAAAGAAGGCAGTGTGACCATTCTTCAAAGTAGTATTAATTCGGCTGAGAGCAACGATCTTAAAGACCTTCTGCCATATGGTATTGCTTTTCATCATGCGGGGTTGAGCAGGGATGAATGCCAAAAAATTGAGGAGCTCTTTGCTGATGGGCGTATACAGGTTTTGGTTTCTACTGCAACACTCGCTTGGGGTGTGAATGTTCGAGCTCATACTGTGATAATAAGAGGAACTCAGGTGTATGATCCAGAGAAGGGTGCATGGGCTGAAATAAGTACTCTGGATGTTATGCAGATGTTAAGTCTTGCAGGAAGACCTCAGTATGATTCAATTGGAGAAGGGGTAATCATTACCGAAGACGACAAACAACAGGACTACTTATCTTTGTTAAACCAAGGAGTTCCTATTCAAAGTTATTTCAGGTCCAGTTTGGAAGATCAATTGAATGCTGAAATCGTCCTTGGGACTGTCAAAAATTTCAGTGAAGCTTGTACATGGTTACAATACACTTACCTGTATGTTCAATGGGAACGTGATCAGAGACTCTGGGGCTTGGAAGCTGCTGATCTACCAAGAGATGTTACATTGAAGGAGAAGATCACTACTGCTGCAAGCATGTTGGCCAGTAAAAAGTTGATCGAGTATGATGAGAAAAGTGGATATTTCCAGGTTACAGACTTGGGTAGAATTGCTAGCTATTACTACATAACTCATACAACAATATCTGCATATTACAAGCATGTGGAGCCAACAATGGAAGGCATCGAGTTTTATCAATTATTCTCCTCTAGTGAGGAATTTAAGTATGTCACTGTTCGGCAGGATGAAAAGATGGAACTAGAATCATTGAATATCGATTTTCGAGTCAAGGAAAGCCTTGAAGAGGCCAGTGGCAAGATTAATGTTTTGTTGCAAGCATATATTTCACAGAAGAAGCTTGAAGAGCTTTCATTGACATATGACATGGTTTACATTACTCAGTGTGCTGAGCGTTTTCTACGAGCTCTTTTTGAGATTATTGTGTTGAAATGTGGAAGAGCTCAACTAGCTGTACAGGCTTTGAACTTGTGTAAAATGGTTAACAAGAGGATGCGGAGTACCGAAGCACCAATTGAGATAAAGCTTGCAGCATCAGTGCAACCAATTACTCGCACTGTATTAAGGGTTGAGCTTACTATTAGTACACCAGACTTCCCATGGGAGGATGGAGTTCATGGATGTGCAGAGCCATTTTGGTTAATAGTGGAAGGTAACAAACAATCTATTCATCATGAATATTTTCTGCTCAAGAATGAGGATCACACATTGAACTTTACAGTGGAAATTCCCGAAGAGGTGGAACCTCAATACGTCATTCACGTGGTGTCTGATAGGTGGGTTCATCAAGAAACTAGTGTGAATCTTAATGTTGAGCACCTTCAGTTACCTGAAAAAAATCCTCCACCTACAGAGTTGGCGGGTTTGAATCAAATTCCTGTGACTGAATTAATGAATCAAGATTATGAAGCTTTGTATGATCTTGAGCATTTTAATCATGTCCAGAGTATGGTCTTCAAACGTCTTTATTACTCCGATGACAATGTCTTGGTTGCTGCACCAACAGGGTGTGGCAAGATTATATGTGCAGAGTTTGCTGTATTGCGGAATCATCAGAAGGATGGTGACAAGATGCGTGTTGTGTATATTACAGCTATGGAATGTCTTGCTAAAGAACGGCACACTGACTGGCAGAGGAGATTCGGAAGATCATCAGATGGTCTCGGGCTGTGTGTTGTTGAATTAACAGGAGACACAGTCACCGACTTGGAACTACTTCAGAAAGGTCAGATTATTATCAGCACTCCTGAGAAATGGGATGCTTTGTCTCGGAGCAAGAAAGGGAGGGGGCATGCTCAAGAGGTTAGTCTTTTTATTATAGATGAAGTTCACTTGATTGTGGGTAAAGATGGCCCAGTTTTTGAAAATATAGTCTCTAGGATGAGATACATATCAAGTAAAGGTTCGAAGAAGATCCGGATTGTGGCTTTGTCCACTTCTCTTGCAAATGCAAATGATGTTGGAGAATGGATAGGGGCTACCTCTCATGACCTTTTCAATTTTGCTCCTGATGCGCGCCCAGTGCCACTGGAATTACACATTCAGGGGGTGGATTTAGCTAATTTTCAAACAGTGATGAATGCACTGGCAAAGCCAACACACACTGCAATTGTTCAACATGGGAAACCTGCTCTTGTGTATGTTCCTATGGGGAAACATGTGCAAGTAACAGCTCTGGATCTGGTGACTTACTCGGATGCAGATGGTGGTGGTACACCATCATTTTCAGAATGGATAATGGAGTCCATAGAGAACATTAGTGATAAAACTTTGAGACGCACTTTGAGCCATGGAGTAGGCTACTTGCATGAAGACTTAACCACTTACGATCAAAACATAGTGTCAGAACTTTTTAAAGCTGACCATATTCAAGTGTGTGTCATGAGTGGTTCAATGTGTTGGGAAGTGCCTTTGTCTGCCCGCTTGGTGGTTGTGATGGGAACCCAATATGATGACCAGGAAAATTTTCATTTTGAATACCCTGTTACTGATCTATTGCAGATGATGGATCATGCTGGTCGCCATAGGAGTGGTGTATCTGGGAAATGTGTCATCTTCTGCCAAGCTTCTCGCAAAGCATTCTACATGAAGTTCTTAAAGGAAGCACTCCCTGTTGAAAGCCGCTTGCACCATTACCTACATGATTATCTAAATGCAGAAGTTGTAGCAGGAATAATTAAGAAGAAGCAAGATGTTGCTGATTACCTCAAACAGACCTTCTTGTACCAGAGGCTTAAGCAAAATCCAAGATATTATAATCTACAGGGGGATCTTTCTGATTACCTCTCACAGCTTGTTACAGATACACTGAGGGAGTTGGAAAAAAGAAGGTTAATCTCTATAGAGAATGGCGAGGATCTTTTACCTTTGTCTTGTGGTAGGATTTGTTCAGACTACTACATCAGATATACTACCGTTGAGCATTTCAGACGTACCTTAACTTCAGAAACAAAGATGAAGGGTCTTCTTGAGATTCTAGCTCATGCTTCAGAGTATTCACAACTTTCTCTACTTCCCGGAGAAGAAGAGCTGATTCAAAGGTTGATTAACCAACAGAGATTTTCCATTGAGATTCCCAACTGCAGAAATCCTCATGTCAAAGCAAATGCTCTGCTTCAGGCCTATTTCTCAAGGAATGAAGTGGGTGGGAACCTATCATCGGACCAGCGTCAGGTCCTCCTTTCTGCAAGTAGGTTGCTGCCTGCAATGGTCTGGATCATTTACAGAAGGGGCTGGCTACAACTTGCTCTCCTAGCTATGAGAGTCAGCCAGATGGTCACTCAAGGAATGTGGGAATATGACTCGATGCTTCTACAGCTTCCCCACTTTACAAAGGAAATGGTAGAGAGATGCCAGCACAATGAGGGAAAGAGGATAAACACTGTATCTGATTTGGTGAACATGAAGAAGGATGAGAGGCATGCACTACTTGAGACCATACTTGAGATGACAGATAACGAGATGGAGGATATTGAAAGATATTGCAGACATTTTCCCAGCAATAAACTGACAAAACTTAAATTTCGGAAGAATGAAAGGGCCGGGGAAATAATAACTTTGGAGATTGAAATAACTACCCATAGACCAGTTGGTGCTGTTGATACTCCAAGGTTTCCAAAGAAGACCAAAGAAGAGGGGTGGTGGCTGGTAGTTGGGGATACCAGTACTAACTCATTGCTTACCATCAAAGAGGTTCCCTTGCAAAAGAAGGCAAAAGTCGAGCTTCAGTTCATGAATTCTGCAATAGCTGGACAGAATCTGGACTTGACCCTATTTTTGATCTCTGATTCACGAATGGGATGCGACAAGGAAAAAAGCTTCACTGTAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000354 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009506 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0046540 GO:0055044 GO:0065003 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C Sec63

Protein Analysis

1632

Amino Acids

185.71

Weight (kDa)

5.9

Isoelectric Point (pI)

39.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	64 - 233	6.5e-17	DEAD/DEAH box helicase
Helicase_C	PF00271	324 - 400	8.3e-07	Helicase conserved C-terminal domain
WHD_SNRNP200	PF23445	461 - 510	5e-10	Winged Helix-turn-helix domain
Sec63	PF02889	548 - 791	1.9e-71	Sec63 Brl domain
DEAD	PF00270	835 - 1007	4.9e-24	DEAD/DEAH box helicase
ResIII	PF04851	840 - 979	1.6e-07	Type III restriction enzyme, res subunit
WHD_SNRNP200	PF23445	1228 - 1304	1.2e-21	Winged Helix-turn-helix domain
Sec63	PF02889	1311 - 1629	7.3e-58	Sec63 Brl domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20960 AT1G20960 AT2G42270
fragaria_vesca	FvH4_5g21940 FvH4_5g30070 FvH4_6g00860 FvH4_6g00860 FvH4_6g47200
malus_domestica	MD04G1240400.v1.1 MD09G1062900.v1.1 MD09G1063000.v1.1 MD12G1257300.v1.1
prunus_persica	Prupe.6G358100_v2.0.a1 Prupe.6G358100_v2.0.a1
pyrus_communis	pycom04g21210 pycom04g21230 pycom111g05330 pycom12g23600 pycom12g23620
rosa_chinensis	RchiOBHm_Chr2g0166221 RchiOBHm_Chr3g0448041 RchiOBHm_Chr3g0448351 RchiOBHm_Chr5g0014801 RchiOBHm_Chr7g0209461
rosa_laevigata	RLG00000003133 RLG00000021598 RLG00000021599 RLG00000025925 RLG00000025950 RLG00000025953 RLG00000032142
rosa_multiflora	Rmu_co8520521.1_g000001 Rmu_sc0005582.1_g000009 Rmu_sc0005969.1_g000018 Rmu_sc0015167.1_g000007 Rmu_ssc0000323.1_g000031
rosa_roxburghii	Rroxscaffold_1G00061750 Rroxscaffold_2G00085080 Rroxscaffold_3G00249430 Rroxscaffold_6G00426200 Rroxscaffold_6G00426480 Rroxscaffold_6G00426530
rosa_rugosa	Rorug02G0521300 Rorug02G0607900 Rorug02G0611500 Rorug02G0611600 Rorug07G0113200
rosa_samantha	Rh2AG587300 Rh2BG599200 Rh2CG569600 Rh2DG608900 Rh3AG010500 Rh3AG010600 Rh3BG010200 Rh3BG010300 Rh3CG009900 Rh3CG010000 Rh3DG010600 Rh3DG010700 Rh5CG124900 Rh7AG247100 Rh7BG242100 Rh7CG262700 Rh7DG253400
rosa_wichuraiana	Rw0G020040 Rw2G048960 Rw3G000580 Rw3G000830 Rw7G020900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3822
Acc36I	ACCTGC	1 cut(s)	3202
Acc65I	GGTACC	1 cut(s)	953
AccB1I	GGYRCC	2 cut(s)	416, 953
AccB7I	CCANNNNNTGG	1 cut(s)	2515
AccI	GTMKAC	1 cut(s)	1126
AccII	CGCG	2 cut(s)	706, 3082
AccIII	TCCGGA	2 cut(s)	85, 2982
AciI	CCGC	7 cut(s)	939, 1070, 2062, 2426, 2611, 3487, 3688
AcoI	YGGCCR	1 cut(s)	1590
AcsI	RAATTY	7 cut(s)	1444, 1766, 2292, 2439, 3529, 4586, 4801
AcuI	CTGAAG	6 cut(s)	2372, 2768, 3993, 4038, 4166, 4775
AcvI	CACGTG	1 cut(s)	2329
AdeI	CACNNNGTG	1 cut(s)	2332
AflII	CTTAAG	1 cut(s)	3800
AflIII	ACRYGT	1 cut(s)	3223
AhlI	ACTAGT	1 cut(s)	2360
AjiI	CACGTC	1 cut(s)	700
AjnI	CCWGG	4 cut(s)	125, 862, 1635, 3522
AjuI	GAANNNNNNNTTGG	2 cut(s)	1413, 1445
Alw21I	GWGCWC	6 cut(s)	232, 1110, 1180, 1975, 2011, 2385
AlwNI	CAGNNNCTG	1 cut(s)	4825
Ama87I	CYCGRG	2 cut(s)	242, 2729
Aor13HI	TCCGGA	2 cut(s)	85, 2982
AoxI	GGCC	8 cut(s)	8, 77, 592, 1590, 1854, 2917, 4185, 4605
ApeKI	GCWGC	5 cut(s)	1529, 1577, 2096, 2561, 4264
ApoI	RAATTY	7 cut(s)	1444, 1766, 2292, 2439, 3529, 4586, 4801
ArsI	GACNNNNNNTTYG	2 cut(s)	4825, 4857
AseI	ATTAAT	3 cut(s)	1008, 1869, 2456
Asp700I	GAANNNNTTC	3 cut(s)	2247, 2781, 3531
Asp718I	GGTACC	1 cut(s)	953
AspLEI	GCGC	3 cut(s)	706, 3082, 3084
AspS9I	GGNCC	8 cut(s)	88, 239, 593, 1392, 2918, 4228, 4240, 4605
AsuC2I	CCSGG	4 cut(s)	243, 244, 4083, 4609
AsuHPI	GGTGA	7 cut(s)	259, 436, 563, 2642, 2758, 3262, 4474
AsuII	TTCGAA	1 cut(s)	2972
AsuNHI	GCTAGC	1 cut(s)	1661
AvaI	CYCGRG	2 cut(s)	242, 2729
AvaII	GGWCC	5 cut(s)	88, 239, 1392, 4228, 4240
AxyI	CCTNAGG	1 cut(s)	501
BaeI	ACNNNNGTAYC	2 cut(s)	927, 960
BalI	TGGCCA	1 cut(s)	1592
BamHI	GGATCC	1 cut(s)	153
BanI	GGYRCC	2 cut(s)	416, 953
BanII	GRGCYC	4 cut(s)	1110, 1180, 1975, 2011
BarI	GAAGNNNNNNTAC	2 cut(s)	3991, 4023
BbrPI	CACGTG	1 cut(s)	2329
BbsI	GAAGAC	7 cut(s)	702, 1288, 1332, 2512, 3384, 4022, 4694
Bbv12I	GWGCWC	6 cut(s)	232, 1110, 1180, 1975, 2011, 2385
BbvI	GCAGC	5 cut(s)	1516, 1564, 2108, 2548, 4251
BceAI	ACGGC	2 cut(s)	92, 2699
BcgI	CGANNNNNNTGC	2 cut(s)	4848, 4882
BciT130I	CCWGG	4 cut(s)	127, 864, 1637, 3524
BciVI	GTATCC	2 cut(s)	928, 4717
BclI	TGATCA	2 cut(s)	516, 1504
BcnI	CCSGG	4 cut(s)	243, 244, 4083, 4609
BcuI	ACTAGT	1 cut(s)	2360
BfmI	CTRYAG	7 cut(s)	360, 2415, 3827, 3906, 4147, 4379, 4890
BfrI	CTTAAG	1 cut(s)	3800
BfuAI	ACCTGC	1 cut(s)	3202
BfuI	GTATCC	2 cut(s)	928, 4717
BisI	GCNGC	6 cut(s)	1530, 1578, 2097, 2562, 3688, 4265
BlpI	GCTNAGC	1 cut(s)	1956
BlsI	GCNGC	6 cut(s)	1531, 1579, 2098, 2563, 3689, 4266
BmcAI	AGTACT	2 cut(s)	1246, 4732
Bme1390I	CCNGG	8 cut(s)	127, 243, 244, 864, 1637, 3524, 4083, 4609
Bme18I	GGWCC	5 cut(s)	88, 239, 1392, 4228, 4240
BmeT110I	CYCGRG	2 cut(s)	242, 2729
BmgBI	CACGTC	1 cut(s)	700
BmgT120I	GGNCC	8 cut(s)	88, 239, 593, 1392, 2918, 4228, 4240, 4605
BmrFI	CCNGG	8 cut(s)	127, 243, 244, 864, 1637, 3524, 4083, 4609
BmrI	ACTGGG	2 cut(s)	2915, 3080
BmtI	GCTAGC	1 cut(s)	1665
BmuI	ACTGGG	2 cut(s)	2915, 3080
BoxI	GACNNNNGTC	1 cut(s)	4233
BpiI	GAAGAC	7 cut(s)	702, 1288, 1332, 2512, 3384, 4022, 4694
BpmI	CTGGAG	2 cut(s)	260, 656
Bpu1102I	GCTNAGC	1 cut(s)	1956
Bpu14I	TTCGAA	1 cut(s)	2972
BpuEI	CTTGAG	9 cut(s)	761, 2240, 2510, 2847, 4055, 4180, 4332, 4517, 4529
BpuMI	CCSGG	4 cut(s)	243, 244, 4083, 4609
Bsa29I	ATCGAT	1 cut(s)	1824
BsaAI	YACGTR	1 cut(s)	2329
BsaBI	GATNNNNATC	2 cut(s)	2801, 2956
BsaI	GGTCTC	4 cut(s)	200, 632, 2732, 4493
BsaWI	WCCGGW	2 cut(s)	85, 2982
BsaXI	ACNNNNNCTCC	6 cut(s)	1296, 1326, 2748, 2778, 4299, 4329
Bse21I	CCTNAGG	1 cut(s)	501
Bse3DI	GCAATG	2 cut(s)	4278, 4739
Bse8I	GATNNNNATC	2 cut(s)	2801, 2956
BseAI	TCCGGA	2 cut(s)	85, 2982
BseBI	CCWGG	4 cut(s)	127, 864, 1637, 3524
BseCI	ATCGAT	1 cut(s)	1824
BseJI	GATNNNNATC	2 cut(s)	2801, 2956
BseMI	GCAATG	2 cut(s)	4278, 4739
BsePI	GCGCGC	1 cut(s)	3080
BseRI	GAGGAG	3 cut(s)	1118, 1744, 2716
BseXI	GCAGC	5 cut(s)	1516, 1564, 2108, 2548, 4251
BseYI	CCCAGC	1 cut(s)	4562
BsgI	GTGCAG	3 cut(s)	2211, 2547, 2613
Bsh1236I	CGCG	2 cut(s)	706, 3082
BshFI	GGCC	8 cut(s)	10, 79, 594, 1592, 1856, 2919, 4187, 4607
BshNI	GGYRCC	2 cut(s)	416, 953
BshVI	ATCGAT	1 cut(s)	1824
BsiHKAI	GWGCWC	6 cut(s)	232, 1110, 1180, 1975, 2011, 2385
BsiHKCI	CYCGRG	2 cut(s)	242, 2729
BsiSI	CCGG	6 cut(s)	42, 86, 243, 2983, 4083, 4608
BslFI	GGGAC	1 cut(s)	1447
BsmBI	CGTCTC	1 cut(s)	3334
BsmFI	GGGAC	1 cut(s)	1447
BsmI	GAATGC	4 cut(s)	1094, 1420, 3151, 3644
BsnI	GGCC	8 cut(s)	10, 79, 594, 1592, 1856, 2919, 4187, 4607
Bso31I	GGTCTC	4 cut(s)	200, 632, 2732, 4493
BsoBI	CYCGRG	2 cut(s)	242, 2729
Bsp119I	TTCGAA	1 cut(s)	2972
Bsp1286I	GDGCHC	6 cut(s)	232, 1110, 1180, 1975, 2011, 2385
Bsp13I	TCCGGA	2 cut(s)	85, 2982
Bsp1407I	TGTACA	3 cut(s)	545, 1460, 2020
Bsp1720I	GCTNAGC	1 cut(s)	1956
Bsp19I	CCATGG	2 cut(s)	2165, 3355
BspACI	CCGC	7 cut(s)	939, 1070, 2062, 2426, 2611, 3487, 3688
BspANI	GGCC	8 cut(s)	10, 79, 594, 1592, 1856, 2919, 4187, 4607
BspDI	ATCGAT	1 cut(s)	1824
BspEI	TCCGGA	2 cut(s)	85, 2982
BspFNI	CGCG	2 cut(s)	706, 3082
BspHI	TCATGA	5 cut(s)	442, 2239, 3052, 3447, 4797
BspMAI	CTGCAG	1 cut(s)	4151
BspMI	ACCTGC	1 cut(s)	3202
BspOI	GCTAGC	1 cut(s)	1665
BspQI	GCTCTTC	3 cut(s)	1905, 1999, 4086
BspT104I	TTCGAA	1 cut(s)	2972
BspT107I	GGYRCC	2 cut(s)	416, 953
BspTI	CTTAAG	1 cut(s)	3800
BspTNI	GGTCTC	4 cut(s)	200, 632, 2732, 4493
BsrDI	GCAATG	2 cut(s)	4278, 4739
BsrGI	TGTACA	3 cut(s)	545, 1460, 2020
BssHII	GCGCGC	1 cut(s)	3080
BssNAI	GTATAC	1 cut(s)	1127
BssT1I	CCWWGG	6 cut(s)	420, 1363, 1429, 2165, 3355, 4673
Bst1107I	GTATAC	1 cut(s)	1127
Bst2UI	CCWGG	4 cut(s)	127, 864, 1637, 3524
Bst6I	CTCTTC	7 cut(s)	468, 1845, 1905, 1999, 2295, 4086, 4692
BstAFI	CTTAAG	1 cut(s)	3800
BstAPI	GCANNNNNTGC	1 cut(s)	2600
BstAUI	TGTACA	3 cut(s)	545, 1460, 2020
BstBAI	YACGTR	1 cut(s)	2329
BstBI	TTCGAA	1 cut(s)	2972
BstDSI	CCRYGG	2 cut(s)	2165, 3355
BstENI	CCTNNNNNAGG	1 cut(s)	1850
BstFNI	CGCG	2 cut(s)	706, 3082
BstHHI	GCGC	3 cut(s)	706, 3082, 3084
BstNI	CCWGG	4 cut(s)	127, 864, 1637, 3524
BstNSI	RCATGY	6 cut(s)	124, 1588, 1712, 2861, 3227, 4490
BstPAI	GACNNNNGTC	1 cut(s)	4233
BstSCI	CCNGG	8 cut(s)	125, 241, 242, 862, 1635, 3522, 4081, 4607
BstSFI	CTRYAG	7 cut(s)	360, 2415, 3827, 3906, 4147, 4379, 4890
BstUI	CGCG	2 cut(s)	706, 3082
BstV1I	GCAGC	5 cut(s)	1516, 1564, 2108, 2548, 4251
BstV2I	GAAGAC	7 cut(s)	702, 1288, 1332, 2512, 3384, 4022, 4694
BstX2I	RGATCY	5 cut(s)	153, 2979, 3244, 3835, 3922
BstXI	CCANNNNNNTGG	1 cut(s)	3596
BstYI	RGATCY	5 cut(s)	153, 2979, 3244, 3835, 3922
BstZ17I	GTATAC	1 cut(s)	1127
Bsu15I	ATCGAT	1 cut(s)	1824
Bsu36I	CCTNAGG	1 cut(s)	501
BsuI	GTATCC	2 cut(s)	928, 4717
BsuRI	GGCC	8 cut(s)	10, 79, 594, 1592, 1856, 2919, 4187, 4607
BsuTUI	ATCGAT	1 cut(s)	1824
BtgI	CCRYGG	2 cut(s)	2165, 3355
BtrI	CACGTC	1 cut(s)	700
BtsI	GCAGTG	2 cut(s)	988, 3168
BveI	ACCTGC	1 cut(s)	3202
CaiI	CAGNNNCTG	1 cut(s)	4825
CciI	TCATGA	5 cut(s)	442, 2239, 3052, 3447, 4797
CfoI	GCGC	3 cut(s)	706, 3082, 3084
Cfr13I	GGNCC	8 cut(s)	88, 239, 593, 1392, 2918, 4228, 4240, 4605
Cfr9I	CCCGGG	1 cut(s)	242
ClaI	ATCGAT	1 cut(s)	1824
CseI	GACGC	2 cut(s)	3351, 4223
CspCI	CAANNNNNGTGG	4 cut(s)	2989, 3024, 4337, 4372
DraI	TTTAAA	1 cut(s)	3421
DraIII	CACNNNGTG	1 cut(s)	2332
EaeI	YGGCCR	1 cut(s)	1590
Eam1104I	CTCTTC	7 cut(s)	468, 1845, 1905, 1999, 2295, 4086, 4692
EarI	CTCTTC	7 cut(s)	468, 1845, 1905, 1999, 2295, 4086, 4692
Ecl136II	GAGCTC	4 cut(s)	1108, 1178, 1973, 2009
Eco130I	CCWWGG	6 cut(s)	420, 1363, 1429, 2165, 3355, 4673
Eco147I	AGGCCT	1 cut(s)	4187
Eco24I	GRGCYC	4 cut(s)	1110, 1180, 1975, 2011
Eco31I	GGTCTC	4 cut(s)	200, 632, 2732, 4493
Eco47I	GGWCC	5 cut(s)	88, 239, 1392, 4228, 4240
Eco53kI	GAGCTC	4 cut(s)	1108, 1178, 1973, 2009
Eco57I	CTGAAG	6 cut(s)	2372, 2768, 3993, 4038, 4166, 4775
Eco72I	CACGTG	1 cut(s)	2329
Eco81I	CCTNAGG	1 cut(s)	501
Eco88I	CYCGRG	2 cut(s)	242, 2729
EcoICRI	GAGCTC	4 cut(s)	1108, 1178, 1973, 2009
EcoNI	CCTNNNNNAGG	1 cut(s)	1850
EcoO109I	RGGNCCY	2 cut(s)	593, 4240
EcoRI	GAATTC	1 cut(s)	4801
EcoRII	CCWGG	4 cut(s)	125, 862, 1635, 3522
EcoT14I	CCWWGG	6 cut(s)	420, 1363, 1429, 2165, 3355, 4673
EcoT22I	ATGCAT	1 cut(s)	967
EcoT38I	GRGCYC	4 cut(s)	1110, 1180, 1975, 2011
ErhI	CCWWGG	6 cut(s)	420, 1363, 1429, 2165, 3355, 4673
Esp3I	CGTCTC	1 cut(s)	3334
FalI	AAGNNNNNCTT	2 cut(s)	4773, 4805
FaqI	GGGAC	1 cut(s)	1447
FauI	CCCGC	3 cut(s)	1063, 2419, 3494
FauNDI	CATATG	2 cut(s)	1048, 1927
FbaI	TGATCA	2 cut(s)	516, 1504
FblI	GTMKAC	1 cut(s)	1126
Fnu4HI	GCNGC	6 cut(s)	1530, 1578, 2097, 2562, 3688, 4265
FriOI	GRGCYC	4 cut(s)	1110, 1180, 1975, 2011
Fsp4HI	GCNGC	6 cut(s)	1530, 1578, 2097, 2562, 3688, 4265
GlaI	GCGC	3 cut(s)	705, 3081, 3083
GluI	GCNGC	6 cut(s)	1530, 1578, 2097, 2562, 3688, 4265
GsaI	CCCAGC	1 cut(s)	4566
GsuI	CTGGAG	2 cut(s)	260, 656
HaeIII	GGCC	8 cut(s)	10, 79, 594, 1592, 1856, 2919, 4187, 4607
HapII	CCGG	6 cut(s)	42, 86, 243, 2983, 4083, 4608
HgaI	GACGC	2 cut(s)	3351, 4223
HhaI	GCGC	3 cut(s)	706, 3082, 3084
Hin6I	GCGC	3 cut(s)	704, 3080, 3082
HinP1I	GCGC	3 cut(s)	704, 3080, 3082
HincII	GTYRAC	1 cut(s)	2050
HindII	GTYRAC	1 cut(s)	2050
HindIII	AAGCTT	6 cut(s)	1455, 1904, 2090, 2475, 3630, 4882
HpaI	GTTAAC	1 cut(s)	2050
HpaII	CCGG	6 cut(s)	42, 86, 243, 2983, 4083, 4608
HphI	GGTGA	7 cut(s)	259, 436, 563, 2642, 2758, 3262, 4474
Hpy99I	CGWCG	1 cut(s)	50
HpyCH4IV	ACGT	8 cut(s)	48, 699, 849, 1501, 2319, 2328, 2527, 3997
HpySE526I	ACGT	8 cut(s)	48, 699, 849, 1501, 2319, 2328, 2527, 3997
HspAI	GCGC	3 cut(s)	704, 3080, 3082
Kpn2I	TCCGGA	2 cut(s)	85, 2982
KpnI	GGTACC	1 cut(s)	957
Ksp22I	TGATCA	2 cut(s)	516, 1504
KspAI	GTTAAC	1 cut(s)	2050
LguI	GCTCTTC	3 cut(s)	1905, 1999, 4086
LmnI	GCTCC	5 cut(s)	235, 1105, 1714, 2838, 3076
Lsp1109I	GCAGC	5 cut(s)	1516, 1564, 2108, 2548, 4251
MaeII	ACGT	8 cut(s)	48, 699, 849, 1501, 2319, 2328, 2527, 3997
MfeI	CAATTG	5 cut(s)	821, 1299, 1409, 2079, 3174
MflI	RGATCY	5 cut(s)	153, 2979, 3244, 3835, 3922
MhlI	GDGCHC	6 cut(s)	232, 1110, 1180, 1975, 2011, 2385
MlsI	TGGCCA	1 cut(s)	1592
MluNI	TGGCCA	1 cut(s)	1592
MmeI	TCCRAC	4 cut(s)	257, 766, 3007, 3864
Mox20I	TGGCCA	1 cut(s)	1592
Mph1103I	ATGCAT	1 cut(s)	967
MroI	TCCGGA	2 cut(s)	85, 2982
MroXI	GAANNNNTTC	3 cut(s)	2247, 2781, 3531
MscI	TGGCCA	1 cut(s)	1592
MslI	CAYNNNNRTG	4 cut(s)	393, 2187, 2327, 3594
Msp20I	TGGCCA	1 cut(s)	1592
MspCI	CTTAAG	1 cut(s)	3800
MspI	CCGG	6 cut(s)	42, 86, 243, 2983, 4083, 4608
MspR9I	CCNGG	8 cut(s)	127, 243, 244, 864, 1637, 3524, 4083, 4609
MunI	CAATTG	5 cut(s)	821, 1299, 1409, 2079, 3174
Mva1269I	GAATGC	4 cut(s)	1094, 1420, 3151, 3644
MvaI	CCWGG	4 cut(s)	127, 864, 1637, 3524
MvnI	CGCG	2 cut(s)	706, 3082
NciI	CCSGG	4 cut(s)	243, 244, 4083, 4609
NcoI	CCATGG	2 cut(s)	2165, 3355
NdeI	CATATG	2 cut(s)	1048, 1927
NheI	GCTAGC	1 cut(s)	1661
NmeAIII	GCCGAG	1 cut(s)	30
NmuCI	GTSAC	9 cut(s)	65, 490, 985, 1777, 2446, 2630, 2764, 3250, 4342
NsiI	ATGCAT	1 cut(s)	967
NspI	RCATGY	6 cut(s)	124, 1588, 1712, 2861, 3227, 4490
NspV	TTCGAA	1 cut(s)	2972
PaeI	GCATGC	2 cut(s)	2861, 4490
PagI	TCATGA	5 cut(s)	442, 2239, 3052, 3447, 4797
PauI	GCGCGC	1 cut(s)	3080
PceI	AGGCCT	1 cut(s)	4187
PciI	ACATGT	1 cut(s)	3223
PciSI	GCTCTTC	3 cut(s)	1905, 1999, 4086
PcsI	WCGNNNNNNNCGW	1 cut(s)	2325
PctI	GAATGC	4 cut(s)	1094, 1420, 3151, 3644
PdmI	GAANNNNTTC	3 cut(s)	2247, 2781, 3531
PflFI	GACNNNGTC	2 cut(s)	2549, 2762
PflMI	CCANNNNNTGG	1 cut(s)	2515
PfoI	TCCNGGA	1 cut(s)	4081
PkrI	GCNGC	6 cut(s)	1531, 1579, 2098, 2563, 3689, 4266
PmaCI	CACGTG	1 cut(s)	2329
PmlI	CACGTG	1 cut(s)	2329
Ppu21I	YACGTR	1 cut(s)	2329
PpuMI	RGGWCCY	1 cut(s)	4240
PscI	ACATGT	1 cut(s)	3223
PshAI	GACNNNNGTC	1 cut(s)	4233
PshBI	ATTAAT	3 cut(s)	1008, 1869, 2456
PsiI	TTATAA	1 cut(s)	3822
Psp124BI	GAGCTC	4 cut(s)	1110, 1180, 1975, 2011
Psp5II	RGGWCCY	1 cut(s)	4240
Psp6I	CCWGG	4 cut(s)	125, 862, 1635, 3522
PspCI	CACGTG	1 cut(s)	2329
PspFI	CCCAGC	1 cut(s)	4562
PspGI	CCWGG	4 cut(s)	125, 862, 1635, 3522
PspPI	GGNCC	8 cut(s)	88, 239, 593, 1392, 2918, 4228, 4240, 4605
PspPPI	RGGWCCY	1 cut(s)	4240
PsrI	GAACNNNNNNTAC	2 cut(s)	3937, 3969
PstI	CTGCAG	1 cut(s)	4151
PstNI	CAGNNNCTG	1 cut(s)	4825
PsuI	RGATCY	5 cut(s)	153, 2979, 3244, 3835, 3922
PsyI	GACNNNGTC	2 cut(s)	2549, 2762
PteI	GCGCGC	1 cut(s)	3080
RseI	CAYNNNNRTG	4 cut(s)	393, 2187, 2327, 3594
SacI	GAGCTC	4 cut(s)	1110, 1180, 1975, 2011
SapI	GCTCTTC	3 cut(s)	1905, 1999, 4086
SatI	GCNGC	6 cut(s)	1530, 1578, 2097, 2562, 3688, 4265
Sau96I	GGNCC	8 cut(s)	88, 239, 593, 1392, 2918, 4228, 4240, 4605
ScaI	AGTACT	2 cut(s)	1246, 4732
ScrFI	CCNGG	8 cut(s)	127, 243, 244, 864, 1637, 3524, 4083, 4609
SduI	GDGCHC	6 cut(s)	232, 1110, 1180, 1975, 2011, 2385
SfcI	CTRYAG	7 cut(s)	360, 2415, 3827, 3906, 4147, 4379, 4890
SfuI	TTCGAA	1 cut(s)	2972
SinI	GGWCC	5 cut(s)	88, 239, 1392, 4228, 4240
SmaI	CCCGGG	1 cut(s)	244
SmiMI	CAYNNNNRTG	4 cut(s)	393, 2187, 2327, 3594
SpeI	ACTAGT	1 cut(s)	2360
SphI	GCATGC	2 cut(s)	2861, 4490
SseBI	AGGCCT	1 cut(s)	4187
SsiI	CCGC	7 cut(s)	939, 1070, 2062, 2426, 2611, 3487, 3688
SspI	AATATT	1 cut(s)	2246
SstI	GAGCTC	4 cut(s)	1110, 1180, 1975, 2011
StuI	AGGCCT	1 cut(s)	4187
StyD4I	CCNGG	8 cut(s)	125, 241, 242, 862, 1635, 3522, 4081, 4607
StyI	CCWWGG	6 cut(s)	420, 1363, 1429, 2165, 3355, 4673
TaiI	ACGT	8 cut(s)	51, 702, 852, 1504, 2322, 2331, 2530, 4000
TaqII	GACCGA	1 cut(s)	224
TatI	WGTACW	6 cut(s)	545, 1244, 1460, 2020, 2150, 4730
TauI	GCSGC	1 cut(s)	3690
TseFI	GTSAC	9 cut(s)	65, 490, 985, 1777, 2446, 2630, 2764, 3250, 4342
TseI	GCWGC	5 cut(s)	1529, 1577, 2096, 2561, 4264
Tsp45I	GTSAC	9 cut(s)	65, 490, 985, 1777, 2446, 2630, 2764, 3250, 4342
TspGWI	ACGGA	1 cut(s)	53
TspMI	CCCGGG	1 cut(s)	242
Tth111I	GACNNNGTC	2 cut(s)	2549, 2762
Van91I	CCANNNNNTGG	1 cut(s)	2515
Vha464I	CTTAAG	1 cut(s)	3800
VpaK11BI	GGWCC	5 cut(s)	88, 239, 1392, 4228, 4240
VspI	ATTAAT	3 cut(s)	1008, 1869, 2456
XagI	CCTNNNNNAGG	1 cut(s)	1850
XapI	RAATTY	7 cut(s)	1444, 1766, 2292, 2439, 3529, 4586, 4801
XceI	RCATGY	6 cut(s)	124, 1588, 1712, 2861, 3227, 4490
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2163, 4652
XmaI	CCCGGG	1 cut(s)	242
XmiI	GTMKAC	1 cut(s)	1126
XmnI	GAANNNNTTC	3 cut(s)	2247, 2781, 3531
ZrmI	AGTACT	2 cut(s)	1246, 4732
Zsp2I	ATGCAT	1 cut(s)	967

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.