Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000802

Physical Location & Seq

Genomic Coordinates Forward (+)

76665897 .. 76668679

Gene Structure 2783 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021598

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGCGGGAGGTGGTGGCGCGGAAGCTCACGCGCGAAGCAACCAATACCAGCCGCGATCGAATTCGAGTCTGGTCCTCCTGGCGTCCGAGTCCGAGTCCGACTCTCGCTCACGTGACAGTCACCTGCCCAGCGGCGAGGCTGAGTCTCTCCGCGGCAGAATCGACCCCAAAAGCTTCGGTGATAGGGTTCGCAGGGGAAGGCCGAAGAGGCAGCGCCGCGACCCGAGTCTCCCGGAGCCTGCTGCTTCGAGCAAACGGCGTCGTATTTGGGAAGAGAGCGTGCTCACCGCGACGGAGGAAGACGGCGTCGTTTACAGGCCGAAGACGGCGCGGCGAGTGTGGTGTACGGCGCCGCCGACGAGGTTCTGGCGGAGATTAAAGTTTGTTCAGATTGGGAAGCTGATCACCGATTATGGTGATGATGATGATGATGATAACGACATAGTTGGTTGTGGTGATGGTGATTTCGATGTGGGAGTTGCGGTTGATTTTGAAGAGGATGAGGAGGAGGGTGTTGGTATGGTTGTGGAGGATGAGGATGAGGATAAGGAGGAACAAAAGGAATCGGTGGCGGGCGGAGTGATTGTTGATGGTGATGGTGTAATGGTGGAATGTAGTGAAGGTGTGAGTGTGAATGTTCATGAGATTGATGCTTACTGGGTGCAGAGGAAGATATATGAGGCTTATGAGAAACAGATTGAGCCACAACAGTGTCAGGAGCTAGCTGAAGAGGTGGTTAGTATACTCGGTGAAGGTGGTGTTAGGGATGTGGAATCCAGGCTTTTGGCGAAGCTCCGGTTTGACAAGTTTGGGCTTATTAAGTTTTTGTTGCTGAACAGGGCGAAGATTGTGTGGTGTACTCGTTTGGCTAGAGCTGGGGGACAGGAGGAGAGGAAGAGAATCGAGGAGGAGATGTTGCGTTCGGGTCCTGATTCGGCTGCAATTGTAGAGGAGCTGTGTTTGATGAGGGCTAGTGTGAAAGAGAGGCAAAAGAGCAAGGAGATGAGTATTAAAGCAGAGGCTCGGAAGTTGAAGAAGGCTGGGGTTGTTGACAGAGACGGAGAAAGTGGTGATTGGTGGAAGAACCAGGATCAGCAGCTTGATCTCGACAGCATTGCACAAGAACAAAGTAGAAGATTTTTGGTGCCAAACAAGTTTGAGCTTCCAAAAGGTTCTTACAGGCATTCAAGCAAGGGATATGAAGAAATCCATGTGCCGGCATTGAAGCCTAGACCTTTCAACTCTGATGAGAAACTTGTGCTAGTATCTGCCATGCCAGAATGGACGCAACCGGCGTTCAAAGGAATGACTCAGCTGAATAGGGTACAAAGTCTAGTATATAGGACTGCCCTTGAAGCAGCGGACAATATTCTCTTATGTGCTCCTACTGGGTCTGGAAAAACTAATGTTGCAGTGCTCACTATACTTCAGCAGTTGGGGCTGCACATGAATCAGGAGGACGGTTCAATAGACCACAGACTTTGGTGTCACGGTGAGGGAGCTAAGTGGAAGTCGGGAGATTATACTTGTACAAAATGGGTCAAGCTTCTCATCATTGACGAAATTCATCTTCTCCAGGATAGTAGAGGCCCGGTTCTTGAAAGTATTGTTGCCCAAACTGTCAGGCAGATTGAGACCACGAAAGAGCATGTCCGCCTGGTGGGATTATCTGCTACACTTCCTAACTATGAAGATGTGGCGTCATTCTTGCGTGTTGATAAGAAGGGTCTGTTTTATTTTGACAATAGTTTCAGACCTATCCCTCTTTCGCAACAGTATGTTGGAATTACTGCAAGGAAGTCATGGCAGAAGTACCAGTTGATGAAGCAACTCTGCTATGAAAAGGTGATGGCTGTAGCTGGAAAACATCAAGTTCTTATTTTTGTACACTCAAGGAAAGCAGCTAAAACAGCTGGTGACATAAGAGATTCTGCACTTGCTAATGATTCCCTTGGTAGATTTCTGAAAGAAGACAGCGGTAGCTGTGAAATCATTCGCAGTGAATTGGTCAAAATCAAGAGCAATGATCTTAAAAATCTTTTACCATATGGTTTTGCTATTCATCATGCTGGTATGGACAGAGCAGACCGCCAACTGGTTGAGGATCTCTTTGCTAAAGGGCATGTACAAGTTTTGGTTTCCACTGCAACGCTTGCTTGGGGTGTGAATCTGCCAGCTCACACTGTGATTATCAAAGGGACTGAAATCTATGATCCAGAAAAGGGAGCATGGACTGAACTAAGTCCGCTTGATGTTATGCAGATGTTGGGTCGTGCAGGAAGACCTCAGTATGATTCTTGTGGAGAAGGGATAATCATTACTGGCCACAAGGAACTACATCACTATCTTTCTTTAATGAACCAACAACTTCCTATTGAAAGTCAATTTCTGTCCAAGTTGGCTGATCAATTGAATGCAGAAATTGTTCTTGGAAATGTTCAGAATGCTAGAGAAGCTTCTCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000354 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009506 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0042623 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045292 GO:0046483 GO:0046540 GO:0055044 GO:0065003 GO:0070013 GO:0070035 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:0140098 GO:1901360 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD Helicase_C Sec63

Protein Analysis

822

Amino Acids

92.13

Weight (kDa)

5.85

Isoelectric Point (pI)

50.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_PWI	PF18149	210 - 319	2.8e-30	N-terminal helicase PWI domain
DEAD	PF00270	441 - 481	2e-06	DEAD/DEAH box helicase
DEAD	PF00270	511 - 565	1.3e-06	DEAD/DEAH box helicase
Helicase_C	PF00271	662 - 762	6.2e-11	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20960 AT1G20960 AT2G42270
fragaria_vesca	FvH4_5g21940 FvH4_5g30070 FvH4_6g00860 FvH4_6g00860 FvH4_6g47200
malus_domestica	MD04G1240400.v1.1 MD09G1062900.v1.1 MD09G1063000.v1.1 MD12G1257300.v1.1
prunus_persica	Prupe.6G358100_v2.0.a1 Prupe.6G358100_v2.0.a1
pyrus_communis	pycom04g21210 pycom04g21230 pycom111g05330 pycom12g23600 pycom12g23620
rosa_chinensis	RchiOBHm_Chr2g0166221 RchiOBHm_Chr3g0448041 RchiOBHm_Chr3g0448351 RchiOBHm_Chr5g0014801 RchiOBHm_Chr7g0209461
rosa_laevigata	RLG00000003133 RLG00000021598 RLG00000021599 RLG00000025925 RLG00000025950 RLG00000025953 RLG00000032142
rosa_multiflora	Rmu_co8520521.1_g000001 Rmu_sc0005582.1_g000009 Rmu_sc0005969.1_g000018 Rmu_sc0015167.1_g000007 Rmu_ssc0000323.1_g000031
rosa_roxburghii	Rroxscaffold_1G00061750 Rroxscaffold_2G00085080 Rroxscaffold_3G00249430 Rroxscaffold_6G00426200 Rroxscaffold_6G00426480 Rroxscaffold_6G00426530
rosa_rugosa	Rorug02G0521300 Rorug02G0607900 Rorug02G0611500 Rorug02G0611600 Rorug07G0113200
rosa_samantha	Rh2AG587300 Rh2BG599200 Rh2CG569600 Rh2DG608900 Rh3AG010500 Rh3AG010600 Rh3BG010200 Rh3BG010300 Rh3CG009900 Rh3CG010000 Rh3DG010600 Rh3DG010700 Rh5CG124900 Rh7AG247100 Rh7BG242100 Rh7CG262700 Rh7DG253400
rosa_wichuraiana	Rw0G020040 Rw2G048960 Rw3G000580 Rw3G000830 Rw7G020900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	132
AasI	GACNNNNNNGTC	1 cut(s)	1485
Acc36I	ACCTGC	1 cut(s)	132
AccB1I	GGYRCC	2 cut(s)	349, 1144
AccI	GTMKAC	1 cut(s)	742
AccII	CGCG	8 cut(s)	20, 32, 34, 55, 153, 219, 290, 331
AclWI	GGATC	3 cut(s)	1098, 2112, 2207
AcoI	YGGCCR	1 cut(s)	2323
AcsI	RAATTY	2 cut(s)	61, 1563
AcuI	CTGAAG	2 cut(s)	747, 1412
AcvI	CACGTG	1 cut(s)	113
AcyI	GRCGYC	5 cut(s)	83, 259, 306, 350, 1700
AdeI	CACNNNGTG	1 cut(s)	2185
AfaI	GTAC	7 cut(s)	346, 859, 1326, 1531, 1814, 1887, 2127
AfiI	CCNNNNNNNGG	4 cut(s)	1216, 1660, 2095, 2222
AjnI	CCWGG	5 cut(s)	78, 776, 1086, 1575, 1656
AjuI	GAANNNNNNNTTGG	2 cut(s)	2412, 2444
AleI	CACNNNNGTG	1 cut(s)	709
Alw21I	GWGCWC	3 cut(s)	285, 1384, 1419
Alw26I	GTCTC	4 cut(s)	150, 233, 1050, 1628
AlwI	GGATC	3 cut(s)	1098, 2112, 2207
Ama87I	CYCGRG	1 cut(s)	223
AoxI	GGCC	4 cut(s)	200, 317, 1588, 2323
ApeKI	GCWGC	7 cut(s)	211, 242, 938, 1096, 1358, 1441, 1901
ApoI	RAATTY	2 cut(s)	61, 1563
Asp700I	GAANNNNTTC	2 cut(s)	1992, 2424
AspLEI	GCGC	5 cut(s)	20, 34, 216, 331, 352
AspS9I	GGNCC	3 cut(s)	73, 926, 1589
AsuC2I	CCSGG	2 cut(s)	233, 1592
AsuNHI	GCTAGC	1 cut(s)	721
AvaI	CYCGRG	1 cut(s)	223
AvaII	GGWCC	2 cut(s)	73, 926
BalI	TGGCCA	1 cut(s)	2325
BanI	GGYRCC	2 cut(s)	349, 1144
BbrPI	CACGTG	1 cut(s)	113
BbsI	GAAGAC	4 cut(s)	306, 329, 1977, 2287
Bbv12I	GWGCWC	3 cut(s)	285, 1384, 1419
BbvI	GCAGC	7 cut(s)	223, 229, 925, 1108, 1370, 1428, 1913
BccI	CCATC	4 cut(s)	452, 584, 590, 1843
BceAI	ACGGC	4 cut(s)	272, 319, 342, 363
BcgI	CGANNNNNNTGC	2 cut(s)	1097, 1131
BciT130I	CCWGG	5 cut(s)	80, 778, 1088, 1577, 1658
BclI	TGATCA	2 cut(s)	402, 2404
BcnI	CCSGG	2 cut(s)	233, 1592
BcoDI	GTCTC	4 cut(s)	150, 233, 1050, 1628
BfaI	CTAG	7 cut(s)	722, 870, 972, 1230, 1262, 1334, 2448
BfmI	CTRYAG	1 cut(s)	1854
BfoI	RGCGCY	2 cut(s)	217, 353
BfuAI	ACCTGC	1 cut(s)	132
BglI	GCCNNNNNGGC	1 cut(s)	208
Bme1390I	CCNGG	7 cut(s)	80, 233, 778, 1088, 1577, 1592, 1658
Bme18I	GGWCC	2 cut(s)	73, 926
BmeT110I	CYCGRG	1 cut(s)	223
BmgT120I	GGNCC	3 cut(s)	73, 926, 1589
BmiI	GGNNCC	4 cut(s)	237, 351, 927, 1146
BmrFI	CCNGG	7 cut(s)	80, 233, 778, 1088, 1577, 1592, 1658
BmrI	ACTGGG	2 cut(s)	667, 1398
BmsI	GCATC	1 cut(s)	640
BmtI	GCTAGC	1 cut(s)	725
BmuI	ACTGGG	2 cut(s)	667, 1398
BoxI	GACNNNNGTC	1 cut(s)	225
BpiI	GAAGAC	4 cut(s)	306, 329, 1977, 2287
BplI	GAGNNNNNCTC	2 cut(s)	86, 118
BpmI	CTGGAG	1 cut(s)	1559
BpuEI	CTTGAG	1 cut(s)	1876
BpuMI	CCSGG	2 cut(s)	233, 1592
BsaAI	YACGTR	1 cut(s)	113
BsaHI	GRCGYC	5 cut(s)	83, 259, 306, 350, 1700
BsaI	GGTCTC	1 cut(s)	1628
BsaJI	CCNNGG	2 cut(s)	151, 1951
BsaWI	WCCGGW	1 cut(s)	795
BsaXI	ACNNNNNCTCC	6 cut(s)	468, 498, 570, 600, 899, 929
Bsc4I	CCNNNNNNNGG	4 cut(s)	1216, 1660, 2095, 2222
Bse118I	RCCGGY	2 cut(s)	1216, 1291
Bse1I	ACTGG	5 cut(s)	662, 1393, 1816, 2100, 2326
Bse3DI	GCAATG	2 cut(s)	1113, 2029
BseBI	CCWGG	5 cut(s)	80, 778, 1088, 1577, 1658
BseDI	CCNNGG	2 cut(s)	151, 1951
BseGI	GGATG	4 cut(s)	505, 538, 544, 772
BseLI	CCNNNNNNNGG	4 cut(s)	1216, 1660, 2095, 2222
BseMI	GCAATG	2 cut(s)	1113, 2029
BseMII	CTCAG	3 cut(s)	132, 1325, 2300
BseNI	ACTGG	5 cut(s)	662, 1393, 1816, 2100, 2326
BseRI	GAGGAG	6 cut(s)	518, 521, 902, 920, 923, 965
BseXI	GCAGC	7 cut(s)	223, 229, 925, 1108, 1370, 1428, 1913
BseYI	CCCAGC	3 cut(s)	128, 875, 1040
BsgI	GTGCAG	4 cut(s)	683, 1427, 1917, 2295
Bsh1236I	CGCG	8 cut(s)	20, 32, 34, 55, 153, 219, 290, 331
Bsh1285I	CGRYCG	1 cut(s)	59
BshFI	GGCC	4 cut(s)	202, 319, 1590, 2325
BshNI	GGYRCC	2 cut(s)	349, 1144
BsiEI	CGRYCG	1 cut(s)	59
BsiHKAI	GWGCWC	3 cut(s)	285, 1384, 1419
BsiHKCI	CYCGRG	1 cut(s)	223
BsiSI	CCGG	5 cut(s)	233, 796, 1217, 1292, 1592
BslFI	GGGAC	2 cut(s)	894, 2212
BslI	CCNNNNNNNGG	4 cut(s)	1216, 1660, 2095, 2222
BsmAI	GTCTC	4 cut(s)	150, 233, 1050, 1628
BsmBI	CGTCTC	1 cut(s)	1050
BsmFI	GGGAC	2 cut(s)	894, 2212
BsmI	GAATGC	3 cut(s)	1183, 2419, 2449
BsnI	GGCC	4 cut(s)	202, 319, 1590, 2325
Bso31I	GGTCTC	1 cut(s)	1628
BsoBI	CYCGRG	1 cut(s)	223
Bsp1286I	GDGCHC	3 cut(s)	285, 1384, 1419
Bsp1407I	TGTACA	3 cut(s)	1529, 1885, 2125
Bsp143I	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
BspANI	GGCC	4 cut(s)	202, 319, 1590, 2325
BspCNI	CTCAG	3 cut(s)	133, 1324, 2299
BspFNI	CGCG	8 cut(s)	20, 32, 34, 55, 153, 219, 290, 331
BspHI	TCATGA	1 cut(s)	640
BspLI	GGNNCC	4 cut(s)	237, 351, 927, 1146
BspMI	ACCTGC	1 cut(s)	132
BspOI	GCTAGC	1 cut(s)	725
BspPI	GGATC	3 cut(s)	1098, 2112, 2207
BspT107I	GGYRCC	2 cut(s)	349, 1144
BspTNI	GGTCTC	1 cut(s)	1628
BsrDI	GCAATG	2 cut(s)	1113, 2029
BsrFI	RCCGGY	2 cut(s)	1216, 1291
BsrGI	TGTACA	3 cut(s)	1529, 1885, 2125
BsrI	ACTGG	5 cut(s)	662, 1393, 1816, 2100, 2326
BssAI	RCCGGY	2 cut(s)	1216, 1291
BssECI	CCNNGG	2 cut(s)	151, 1951
BssMI	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
BssNAI	GTATAC	1 cut(s)	743
BssNI	GRCGYC	5 cut(s)	83, 259, 306, 350, 1700
BssT1I	CCWWGG	1 cut(s)	1951
Bst1107I	GTATAC	1 cut(s)	743
Bst2UI	CCWGG	5 cut(s)	80, 778, 1088, 1577, 1658
Bst4CI	ACNGT	7 cut(s)	119, 711, 1463, 1493, 1621, 1776, 2185
Bst6I	CTCTTC	5 cut(s)	200, 267, 489, 723, 890
BstACI	GRCGYC	5 cut(s)	83, 259, 306, 350, 1700
BstAPI	GCANNNNNTGC	1 cut(s)	2153
BstAUI	TGTACA	3 cut(s)	1529, 1885, 2125
BstBAI	YACGTR	1 cut(s)	113
BstC8I	GCNNGC	7 cut(s)	240, 281, 574, 723, 1218, 2154, 2175
BstDEI	CTNAG	5 cut(s)	141, 1311, 1503, 2240, 2286
BstDSI	CCRYGG	1 cut(s)	151
BstF5I	GGATG	4 cut(s)	505, 538, 544, 772
BstFNI	CGCG	8 cut(s)	20, 32, 34, 55, 153, 219, 290, 331
BstH2I	RGCGCY	2 cut(s)	217, 353
BstHHI	GCGC	5 cut(s)	20, 34, 216, 331, 352
BstKTI	GATC	8 cut(s)	59, 405, 1093, 1105, 2029, 2107, 2215, 2407
BstMAI	GTCTC	4 cut(s)	150, 233, 1050, 1628
BstMBI	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
BstMCI	CGRYCG	1 cut(s)	59
BstMWI	GCNNNNNNNGC	6 cut(s)	208, 1355, 1438, 1910, 2153, 2453
BstNI	CCWGG	5 cut(s)	80, 778, 1088, 1577, 1658
BstNSI	RCATGY	2 cut(s)	1652, 2126
BstPAI	GACNNNNGTC	1 cut(s)	225
BstSCI	CCNGG	7 cut(s)	78, 231, 776, 1086, 1575, 1590, 1656
BstSFI	CTRYAG	1 cut(s)	1854
BstUI	CGCG	8 cut(s)	20, 32, 34, 55, 153, 219, 290, 331
BstV1I	GCAGC	7 cut(s)	223, 229, 925, 1108, 1370, 1428, 1913
BstV2I	GAAGAC	4 cut(s)	306, 329, 1977, 2287
BstX2I	RGATCY	1 cut(s)	2104
BstXI	CCANNNNNNTGG	1 cut(s)	784
BstYI	RGATCY	1 cut(s)	2104
BstZ17I	GTATAC	1 cut(s)	743
BsuRI	GGCC	4 cut(s)	202, 319, 1590, 2325
BtgI	CCRYGG	1 cut(s)	151
BtsCI	GGATG	4 cut(s)	505, 538, 544, 772
BtsI	GCAGTG	3 cut(s)	1419, 2005, 2142
BtsIMutI	CAGTG	5 cut(s)	716, 1419, 2005, 2142, 2181
BveI	ACCTGC	1 cut(s)	132
Cac8I	GCNNGC	7 cut(s)	240, 281, 574, 723, 1218, 2154, 2175
CciI	TCATGA	1 cut(s)	640
CfoI	GCGC	5 cut(s)	20, 34, 216, 331, 352
Cfr10I	RCCGGY	2 cut(s)	1216, 1291
Cfr13I	GGNCC	3 cut(s)	73, 926, 1589
Cfr42I	CCGCGG	1 cut(s)	154
CseI	GACGC	5 cut(s)	72, 248, 295, 1294, 1689
Csp6I	GTAC	7 cut(s)	345, 858, 1325, 1530, 1813, 1886, 2126
CspCI	CAANNNNNGTGG	2 cut(s)	1463, 1498
CviAII	CATG	9 cut(s)	641, 1211, 1273, 1447, 1649, 1803, 2066, 2123, 2229
CviQI	GTAC	7 cut(s)	345, 858, 1325, 1530, 1813, 1886, 2126
DdeI	CTNAG	5 cut(s)	141, 1311, 1503, 2240, 2286
DinI	GGCGCC	1 cut(s)	351
DpnI	GATC	8 cut(s)	58, 404, 1092, 1104, 2028, 2106, 2214, 2406
DpnII	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
DraIII	CACNNNGTG	1 cut(s)	2185
DrdI	GACNNNNNNGTC	1 cut(s)	1485
DseDI	GACNNNNNNGTC	1 cut(s)	1485
EaeI	YGGCCR	1 cut(s)	2323
Eam1104I	CTCTTC	5 cut(s)	200, 267, 489, 723, 890
EarI	CTCTTC	5 cut(s)	200, 267, 489, 723, 890
EciI	GGCGGA	3 cut(s)	385, 591, 1643
Eco130I	CCWWGG	1 cut(s)	1951
Eco31I	GGTCTC	1 cut(s)	1628
Eco47I	GGWCC	2 cut(s)	73, 926
Eco57I	CTGAAG	2 cut(s)	747, 1412
Eco72I	CACGTG	1 cut(s)	113
Eco88I	CYCGRG	1 cut(s)	223
EcoO109I	RGGNCCY	1 cut(s)	926
EcoRI	GAATTC	1 cut(s)	61
EcoRII	CCWGG	5 cut(s)	78, 776, 1086, 1575, 1656
EcoT14I	CCWWGG	1 cut(s)	1951
EgeI	GGCGCC	1 cut(s)	351
EheI	GGCGCC	1 cut(s)	351
ErhI	CCWWGG	1 cut(s)	1951
Esp3I	CGTCTC	1 cut(s)	1050
FaeI	CATG	9 cut(s)	644, 1214, 1276, 1450, 1652, 1806, 2069, 2126, 2232
FaqI	GGGAC	2 cut(s)	894, 2212
FatI	CATG	9 cut(s)	640, 1210, 1272, 1446, 1648, 1802, 2065, 2122, 2228
FauI	CCCGC	1 cut(s)	565
FauNDI	CATATG	1 cut(s)	2047
FbaI	TGATCA	2 cut(s)	402, 2404
FblI	GTMKAC	1 cut(s)	742
FokI	GGATG	4 cut(s)	512, 545, 551, 779
FspBI	CTAG	7 cut(s)	722, 870, 972, 1230, 1262, 1334, 2448
GlaI	GCGC	5 cut(s)	19, 33, 215, 330, 351
GsaI	CCCAGC	3 cut(s)	132, 879, 1044
GsuI	CTGGAG	1 cut(s)	1559
HaeII	RGCGCY	2 cut(s)	217, 353
HaeIII	GGCC	4 cut(s)	202, 319, 1590, 2325
HapII	CCGG	5 cut(s)	233, 796, 1217, 1292, 1592
HgaI	GACGC	5 cut(s)	72, 248, 295, 1294, 1689
HhaI	GCGC	5 cut(s)	20, 34, 216, 331, 352
Hin1I	GRCGYC	5 cut(s)	83, 259, 306, 350, 1700
Hin1II	CATG	9 cut(s)	644, 1214, 1276, 1450, 1652, 1806, 2069, 2126, 2232
Hin6I	GCGC	5 cut(s)	18, 32, 214, 329, 350
HinP1I	GCGC	5 cut(s)	18, 32, 214, 329, 350
HincII	GTYRAC	1 cut(s)	1051
HindII	GTYRAC	1 cut(s)	1051
HindIII	AAGCTT	3 cut(s)	172, 1544, 2454
HpaII	CCGG	5 cut(s)	233, 796, 1217, 1292, 1592
Hpy166II	GTNNAC	6 cut(s)	313, 345, 743, 858, 1051, 1888
Hpy188I	TCNGA	9 cut(s)	88, 94, 100, 390, 1026, 1246, 1754, 1965, 2442
Hpy8I	GTNNAC	6 cut(s)	313, 345, 743, 858, 1051, 1888
Hpy99I	CGWCG	4 cut(s)	264, 295, 311, 361
HpyAV	CCTTC	6 cut(s)	192, 614, 746, 1030, 1717, 2300
HpyCH4III	ACNGT	7 cut(s)	119, 711, 1463, 1493, 1621, 1776, 2185
HpyCH4IV	ACGT	1 cut(s)	112
HpyF10VI	GCNNNNNNNGC	6 cut(s)	208, 1355, 1438, 1910, 2153, 2453
HpyF3I	CTNAG	5 cut(s)	141, 1311, 1503, 2240, 2286
HpySE526I	ACGT	1 cut(s)	112
Hsp92I	GRCGYC	5 cut(s)	83, 259, 306, 350, 1700
Hsp92II	CATG	9 cut(s)	644, 1214, 1276, 1450, 1652, 1806, 2069, 2126, 2232
HspAI	GCGC	5 cut(s)	18, 32, 214, 329, 350
KasI	GGCGCC	1 cut(s)	349
KroI	GCCGGC	1 cut(s)	1216
KroNI	GCCGGC	1 cut(s)	1218
Ksp22I	TGATCA	2 cut(s)	402, 2404
KspI	CCGCGG	1 cut(s)	154
Kzo9I	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
LmnI	GCTCC	7 cut(s)	235, 718, 798, 952, 1387, 1499, 2225
Lsp1109I	GCAGC	7 cut(s)	223, 229, 925, 1108, 1370, 1428, 1913
LweI	GCATC	1 cut(s)	640
MaeI	CTAG	7 cut(s)	722, 870, 972, 1230, 1262, 1334, 2448
MaeII	ACGT	1 cut(s)	112
MaeIII	GTNAC	4 cut(s)	113, 119, 1487, 1916
MalI	GATC	8 cut(s)	58, 404, 1092, 1104, 2028, 2106, 2214, 2406
MboI	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
MfeI	CAATTG	2 cut(s)	942, 2408
MflI	RGATCY	1 cut(s)	2104
MhlI	GDGCHC	3 cut(s)	285, 1384, 1419
MlsI	TGGCCA	1 cut(s)	2325
MluCI	AATT	8 cut(s)	61, 942, 1563, 1785, 2003, 2384, 2408, 2421
MluNI	TGGCCA	1 cut(s)	2325
Mly113I	GGCGCC	1 cut(s)	350
MlyI	GAGTC	7 cut(s)	76, 95, 98, 104, 152, 235, 1303
MmeI	TCCRAC	2 cut(s)	123, 1762
Mox20I	TGGCCA	1 cut(s)	2325
MroNI	GCCGGC	1 cut(s)	1216
MroXI	GAANNNNTTC	2 cut(s)	1992, 2424
MscI	TGGCCA	1 cut(s)	2325
MseI	TTAA	5 cut(s)	377, 819, 1011, 2031, 2354
MslI	CAYNNNNRTG	1 cut(s)	709
Msp20I	TGGCCA	1 cut(s)	2325
MspA1I	CMGCKG	6 cut(s)	132, 153, 1315, 1361, 1913, 1977
MspI	CCGG	5 cut(s)	233, 796, 1217, 1292, 1592
MspR9I	CCNGG	7 cut(s)	80, 233, 778, 1088, 1577, 1592, 1658
MunI	CAATTG	2 cut(s)	942, 2408
Mva1269I	GAATGC	3 cut(s)	1183, 2419, 2449
MvaI	CCWGG	5 cut(s)	80, 778, 1088, 1577, 1658
MvnI	CGCG	8 cut(s)	20, 32, 34, 55, 153, 219, 290, 331
MwoI	GCNNNNNNNGC	6 cut(s)	208, 1355, 1438, 1910, 2153, 2453
NaeI	GCCGGC	1 cut(s)	1218
NarI	GGCGCC	1 cut(s)	350
NciI	CCSGG	2 cut(s)	233, 1592
NdeI	CATATG	1 cut(s)	2047
NdeII	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
NgoMIV	GCCGGC	1 cut(s)	1216
NheI	GCTAGC	1 cut(s)	721
NlaIII	CATG	9 cut(s)	644, 1214, 1276, 1450, 1652, 1806, 2069, 2126, 2232
NlaIV	GGNNCC	4 cut(s)	237, 351, 927, 1146
NmuCI	GTSAC	4 cut(s)	113, 119, 1487, 1916
NspI	RCATGY	2 cut(s)	1652, 2126
OliI	CACNNNNGTG	1 cut(s)	709
PagI	TCATGA	1 cut(s)	640
PaqCI	CACCTGC	1 cut(s)	132
PcsI	WCGNNNNNNNCGW	2 cut(s)	353, 1292
PctI	GAATGC	3 cut(s)	1183, 2419, 2449
PdiI	GCCGGC	1 cut(s)	1218
PdmI	GAANNNNTTC	2 cut(s)	1992, 2424
PflFI	GACNNNGTC	1 cut(s)	305
PfoI	TCCNGGA	2 cut(s)	231, 1575
Ple19I	CGATCG	1 cut(s)	59
PleI	GAGTC	7 cut(s)	75, 95, 97, 103, 151, 234, 1303
PluTI	GGCGCC	1 cut(s)	353
PmaCI	CACGTG	1 cut(s)	113
PmlI	CACGTG	1 cut(s)	113
PpsI	GAGTC	7 cut(s)	75, 95, 97, 103, 151, 234, 1303
Ppu21I	YACGTR	1 cut(s)	113
PpuMI	RGGWCCY	1 cut(s)	926
PshAI	GACNNNNGTC	1 cut(s)	225
Psp5II	RGGWCCY	1 cut(s)	926
Psp6I	CCWGG	5 cut(s)	78, 776, 1086, 1575, 1656
PspCI	CACGTG	1 cut(s)	113
PspFI	CCCAGC	3 cut(s)	128, 875, 1040
PspGI	CCWGG	5 cut(s)	78, 776, 1086, 1575, 1656
PspN4I	GGNNCC	4 cut(s)	237, 351, 927, 1146
PspPI	GGNCC	3 cut(s)	73, 926, 1589
PspPPI	RGGWCCY	1 cut(s)	926
PsuI	RGATCY	1 cut(s)	2104
PsyI	GACNNNGTC	1 cut(s)	305
PvuI	CGATCG	1 cut(s)	59
PvuII	CAGCTG	2 cut(s)	1315, 1913
RsaI	GTAC	7 cut(s)	346, 859, 1326, 1531, 1814, 1887, 2127
RsaNI	GTAC	7 cut(s)	345, 858, 1325, 1530, 1813, 1886, 2126
RseI	CAYNNNNRTG	1 cut(s)	709
SacII	CCGCGG	1 cut(s)	154
SaqAI	TTAA	5 cut(s)	377, 819, 1011, 2031, 2354
Sau3AI	GATC	8 cut(s)	56, 402, 1090, 1102, 2026, 2104, 2212, 2404
Sau96I	GGNCC	3 cut(s)	73, 926, 1589
SchI	GAGTC	7 cut(s)	76, 95, 98, 104, 152, 235, 1303
ScrFI	CCNGG	7 cut(s)	80, 233, 778, 1088, 1577, 1592, 1658
SduI	GDGCHC	3 cut(s)	285, 1384, 1419
SfaNI	GCATC	1 cut(s)	640
SfcI	CTRYAG	1 cut(s)	1854
SfoI	GGCGCC	1 cut(s)	351
Sfr303I	CCGCGG	1 cut(s)	154
SgrBI	CCGCGG	1 cut(s)	154
SinI	GGWCC	2 cut(s)	73, 926
SmiMI	CAYNNNNRTG	1 cut(s)	709
SmlI	CTYRAG	1 cut(s)	1891
SmoI	CTYRAG	1 cut(s)	1891
Sse9I	AATT	8 cut(s)	61, 942, 1563, 1785, 2003, 2384, 2408, 2421
SspDI	GGCGCC	1 cut(s)	349
SspI	AATATT	1 cut(s)	1369
SspMI	CTAG	7 cut(s)	722, 870, 972, 1230, 1262, 1334, 2448
StyD4I	CCNGG	7 cut(s)	78, 231, 776, 1086, 1575, 1590, 1656
StyI	CCWWGG	1 cut(s)	1951
TaaI	ACNGT	7 cut(s)	119, 711, 1463, 1493, 1621, 1776, 2185
TaiI	ACGT	1 cut(s)	115
TaqI	TCGA	7 cut(s)	59, 65, 162, 248, 468, 903, 1107
TasI	AATT	8 cut(s)	61, 942, 1563, 1785, 2003, 2384, 2408, 2421
TatI	WGTACW	4 cut(s)	857, 1529, 1885, 2125
TauI	GCSGC	6 cut(s)	55, 135, 156, 219, 334, 355
Tru1I	TTAA	5 cut(s)	377, 819, 1011, 2031, 2354
Tru9I	TTAA	5 cut(s)	377, 819, 1011, 2031, 2354
TscAI	CASTG	5 cut(s)	716, 1419, 2005, 2149, 2188
TseFI	GTSAC	4 cut(s)	113, 119, 1487, 1916
TseI	GCWGC	7 cut(s)	211, 242, 938, 1096, 1358, 1441, 1901
Tsp45I	GTSAC	4 cut(s)	113, 119, 1487, 1916
TspDTI	ATGAA	9 cut(s)	629, 1215, 1463, 1556, 1704, 1838, 1854, 2051, 2372
TspGWI	ACGGA	2 cut(s)	308, 1074
TspRI	CASTG	5 cut(s)	716, 1419, 2005, 2149, 2188
Tth111I	GACNNNGTC	1 cut(s)	305
VpaK11BI	GGWCC	2 cut(s)	73, 926
XapI	RAATTY	2 cut(s)	61, 1563
XceI	RCATGY	2 cut(s)	1652, 2126
XmiI	GTMKAC	1 cut(s)	742
XmnI	GAANNNNTTC	2 cut(s)	1992, 2424
XspI	CTAG	7 cut(s)	722, 870, 972, 1230, 1262, 1334, 2448

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.