U5 small nuclear ribonucleoprotein 200 kDa
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| BRR2_plug | PF21188 | 120 - 185 | 5.4e-09 | Pre-mRNA-splicing helicase BRR2 plug domain |
| Helicase_PWI | PF18149 | 277 - 387 | 8.9e-34 | N-terminal helicase PWI domain |
| ResIII | PF04851 | 516 - 691 | 2e-12 | Type III restriction enzyme, res subunit |
| DEAD | PF00270 | 517 - 695 | 2.3e-32 | DEAD/DEAH box helicase |
| Helicase_C | PF00271 | 804 - 892 | 2.6e-09 | Helicase conserved C-terminal domain |
| WHD_SNRNP200 | PF23445 | 923 - 999 | 1.4e-22 | Winged Helix-turn-helix domain |
| Sec63 | PF02889 | 1015 - 1318 | 7.4e-100 | Sec63 Brl domain |
| DEAD | PF00270 | 1360 - 1535 | 1.9e-30 | DEAD/DEAH box helicase |
| ResIII | PF04851 | 1360 - 1515 | 5.7e-12 | Type III restriction enzyme, res subunit |
| WHD_SNRNP200 | PF23445 | 1763 - 1844 | 4.5e-38 | Winged Helix-turn-helix domain |
| Sec63 | PF02889 | 1850 - 2164 | 5.1e-71 | Sec63 Brl domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1713, 5427 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 5187 |
| Acc36I | ACCTGC | 4 cut(s) | 271, 1221, 4084, 4438 |
| AccB1I | GGYRCC | 1 cut(s) | 288 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 4963, 5095 |
| AccI | GTMKAC | 4 cut(s) | 1363, 1566, 5012, 6437 |
| AccIII | TCCGGA | 1 cut(s) | 1681 |
| AcoI | YGGCCR | 2 cut(s) | 101, 5125 |
| AcsI | RAATTY | 6 cut(s) | 2379, 3167, 3870, 4039, 4560, 4708 |
| AcvI | CACGTG | 1 cut(s) | 107 |
| AcyI | GRCGYC | 3 cut(s) | 289, 3367, 3479 |
| AflII | CTTAAG | 2 cut(s) | 218, 461 |
| AflIII | ACRYGT | 3 cut(s) | 3664, 4318, 4438 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 5008 |
| AjiI | CACGTC | 1 cut(s) | 6079 |
| AjnI | CCWGG | 7 cut(s) | 579, 708, 1317, 3545, 4654, 5381, 5698 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2802, 2834, 3524, 3556 |
| Alw21I | GWGCWC | 3 cut(s) | 315, 1061, 3382 |
| Alw26I | GTCTC | 8 cut(s) | 350, 464, 1248, 1844, 2018, 4525, 4525, 6125 |
| Alw44I | GTGCAC | 1 cut(s) | 1057 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 6134, 6422 |
| Ama87I | CYCGRG | 2 cut(s) | 73, 360 |
| Aor13HI | TCCGGA | 1 cut(s) | 1681 |
| ApaLI | GTGCAC | 1 cut(s) | 1057 |
| ApeKI | GCWGC | 8 cut(s) | 7, 295, 397, 412, 2978, 3659, 3670, 4139 |
| ApoI | RAATTY | 6 cut(s) | 2379, 3167, 3870, 4039, 4560, 4708 |
| AseI | ATTAAT | 1 cut(s) | 3276 |
| Asp700I | GAANNNNTTC | 6 cut(s) | 1049, 2382, 2814, 3882, 6105, 6117 |
| AspLEI | GCGC | 5 cut(s) | 37, 291, 1610, 4665, 4956 |
| AsuC2I | CCSGG | 4 cut(s) | 43, 361, 362, 5129 |
| AsuHPI | GGTGA | 8 cut(s) | 185, 632, 968, 1829, 1885, 2245, 4525, 4562 |
| AsuII | TTCGAA | 1 cut(s) | 5719 |
| AsuNHI | GCTAGC | 1 cut(s) | 3062 |
| AvaI | CYCGRG | 2 cut(s) | 73, 360 |
| AvaII | GGWCC | 9 cut(s) | 82, 706, 1153, 2827, 4298, 4499, 5385, 5845, 5857 |
| BaeGI | GKGCMC | 1 cut(s) | 1061 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 534, 567 |
| BanI | GGYRCC | 1 cut(s) | 288 |
| BanII | GRGCYC | 5 cut(s) | 1516, 3264, 3324, 3382, 3415 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 5252, 5284 |
| BauI | CACGAG | 2 cut(s) | 116, 2304 |
| BbrPI | CACGTG | 1 cut(s) | 107 |
| BbsI | GAAGAC | 7 cut(s) | 1086, 2367, 2677, 3362, 4090, 5639, 6437 |
| Bbv12I | GWGCWC | 3 cut(s) | 315, 1061, 3382 |
| BbvI | GCAGC | 8 cut(s) | 19, 282, 409, 424, 2965, 3671, 3682, 4126 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1109, 1143 |
| BciT130I | CCWGG | 7 cut(s) | 581, 710, 1319, 3547, 4656, 5383, 5700 |
| BciVI | GTATCC | 3 cut(s) | 3993, 6300, 6325 |
| BclI | TGATCA | 3 cut(s) | 567, 4996, 5727 |
| BcnI | CCSGG | 4 cut(s) | 43, 361, 362, 5129 |
| BcoDI | GTCTC | 8 cut(s) | 350, 464, 1248, 1844, 2018, 4525, 4525, 6125 |
| BfaI | CTAG | 7 cut(s) | 1176, 1269, 2004, 3063, 4524, 5837, 5936 |
| BfmI | CTRYAG | 5 cut(s) | 606, 4893, 5523, 6414, 6495 |
| BfoI | RGCGCY | 1 cut(s) | 292 |
| BfrI | CTTAAG | 2 cut(s) | 218, 461 |
| BfuAI | ACCTGC | 4 cut(s) | 271, 1221, 4084, 4438 |
| BfuI | GTATCC | 3 cut(s) | 3993, 6300, 6325 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1172 |
| Bme18I | GGWCC | 9 cut(s) | 82, 706, 1153, 2827, 4298, 4499, 5385, 5845, 5857 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 5008 |
| BmeT110I | CYCGRG | 2 cut(s) | 73, 360 |
| BmgBI | CACGTC | 1 cut(s) | 6079 |
| BmiI | GGNNCC | 4 cut(s) | 290, 3756, 4501, 6411 |
| BmrI | ACTGGG | 3 cut(s) | 1626, 3258, 4661 |
| BmtI | GCTAGC | 1 cut(s) | 3066 |
| BmuI | ACTGGG | 3 cut(s) | 1626, 3258, 4661 |
| BoxI | GACNNNNGTC | 3 cut(s) | 760, 3051, 4094 |
| BpiI | GAAGAC | 7 cut(s) | 1086, 2367, 2677, 3362, 4090, 5639, 6437 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1306, 1338, 6355, 6387 |
| BpmI | CTGGAG | 1 cut(s) | 4377 |
| Bpu10I | CCTNAGC | 1 cut(s) | 5196 |
| Bpu14I | TTCGAA | 1 cut(s) | 5719 |
| BpuEI | CTTGAG | 4 cut(s) | 2263, 2914, 4385, 5672 |
| BpuMI | CCSGG | 4 cut(s) | 43, 361, 362, 5129 |
| BsaAI | YACGTR | 3 cut(s) | 107, 2891, 4319 |
| BsaBI | GATNNNNATC | 2 cut(s) | 3843, 4382 |
| BsaHI | GRCGYC | 3 cut(s) | 289, 3367, 3479 |
| BsaI | GGTCTC | 2 cut(s) | 350, 2018 |
| BsaWI | WCCGGW | 1 cut(s) | 1681 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1100, 1130 |
| Bse118I | RCCGGY | 2 cut(s) | 291, 1038 |
| Bse3DI | GCAATG | 5 cut(s) | 2189, 2419, 5895, 5917, 5946 |
| Bse8I | GATNNNNATC | 2 cut(s) | 3843, 4382 |
| BseAI | TCCGGA | 1 cut(s) | 1681 |
| BseBI | CCWGG | 7 cut(s) | 581, 710, 1319, 3547, 4656, 5383, 5700 |
| BseJI | GATNNNNATC | 2 cut(s) | 3843, 4382 |
| BseMI | GCAATG | 5 cut(s) | 2189, 2419, 5895, 5917, 5946 |
| BseSI | GKGCMC | 1 cut(s) | 1061 |
| BseXI | GCAGC | 8 cut(s) | 19, 282, 409, 424, 2965, 3671, 3682, 4126 |
| BseYI | CCCAGC | 4 cut(s) | 1415, 2240, 3362, 6221 |
| BsgI | GTGCAG | 5 cut(s) | 1655, 2685, 3689, 4125, 4191 |
| BshNI | GGYRCC | 1 cut(s) | 288 |
| BsiHKAI | GWGCWC | 3 cut(s) | 315, 1061, 3382 |
| BsiHKCI | CYCGRG | 2 cut(s) | 73, 360 |
| BsiSI | CCGG | 8 cut(s) | 43, 134, 200, 292, 361, 1039, 1682, 5128 |
| BslFI | GGGAC | 5 cut(s) | 1274, 2132, 3143, 4485, 5193 |
| BsmAI | GTCTC | 8 cut(s) | 350, 464, 1248, 1844, 2018, 4525, 4525, 6125 |
| BsmBI | CGTCTC | 2 cut(s) | 464, 1248 |
| BsmFI | GGGAC | 5 cut(s) | 1274, 2132, 3143, 4485, 5193 |
| BsmI | GAATGC | 3 cut(s) | 2809, 3603, 5276 |
| Bso31I | GGTCTC | 2 cut(s) | 350, 2018 |
| BsoBI | CYCGRG | 2 cut(s) | 73, 360 |
| Bsp119I | TTCGAA | 1 cut(s) | 5719 |
| Bsp1286I | GDGCHC | 7 cut(s) | 315, 1061, 1516, 3264, 3324, 3382, 3415 |
| Bsp13I | TCCGGA | 1 cut(s) | 1681 |
| Bsp1407I | TGTACA | 3 cut(s) | 2515, 5394, 6502 |
| Bsp19I | CCATGG | 2 cut(s) | 3496, 4957 |
| BspEI | TCCGGA | 1 cut(s) | 1681 |
| BspHI | TCATGA | 3 cut(s) | 1966, 3805, 5049 |
| BspLI | GGNNCC | 4 cut(s) | 290, 3756, 4501, 6411 |
| BspMAI | CTGCAG | 3 cut(s) | 610, 4897, 6418 |
| BspMI | ACCTGC | 4 cut(s) | 271, 1221, 4084, 4438 |
| BspOI | GCTAGC | 1 cut(s) | 3066 |
| BspQI | GCTCTTC | 2 cut(s) | 1307, 3252 |
| BspT104I | TTCGAA | 1 cut(s) | 5719 |
| BspT107I | GGYRCC | 1 cut(s) | 288 |
| BspTI | CTTAAG | 2 cut(s) | 218, 461 |
| BspTNI | GGTCTC | 2 cut(s) | 350, 2018 |
| BsrDI | GCAATG | 5 cut(s) | 2189, 2419, 5895, 5917, 5946 |
| BsrFI | RCCGGY | 2 cut(s) | 291, 1038 |
| BsrGI | TGTACA | 3 cut(s) | 2515, 5394, 6502 |
| BssAI | RCCGGY | 2 cut(s) | 291, 1038 |
| BssNAI | GTATAC | 1 cut(s) | 6438 |
| BssNI | GRCGYC | 3 cut(s) | 289, 3367, 3479 |
| BssSI | CACGAG | 2 cut(s) | 116, 2304 |
| BssT1I | CCWWGG | 8 cut(s) | 2341, 3496, 4257, 4599, 4957, 6284, 6300, 6387 |
| Bst1107I | GTATAC | 1 cut(s) | 6438 |
| Bst2BI | CACGAG | 2 cut(s) | 116, 2304 |
| Bst2UI | CCWGG | 7 cut(s) | 581, 710, 1319, 3547, 4656, 5383, 5700 |
| BstACI | GRCGYC | 3 cut(s) | 289, 3367, 3479 |
| BstAFI | CTTAAG | 2 cut(s) | 218, 461 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 4178, 4862 |
| BstAUI | TGTACA | 3 cut(s) | 2515, 5394, 6502 |
| BstBAI | YACGTR | 3 cut(s) | 107, 2891, 4319 |
| BstBI | TTCGAA | 1 cut(s) | 5719 |
| BstDSI | CCRYGG | 3 cut(s) | 321, 3496, 4957 |
| BstEII | GGTNACC | 2 cut(s) | 1817, 5831 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 4245 |
| BstH2I | RGCGCY | 1 cut(s) | 292 |
| BstHHI | GCGC | 5 cut(s) | 37, 291, 1610, 4665, 4956 |
| BstMAI | GTCTC | 8 cut(s) | 350, 464, 1248, 1844, 2018, 4525, 4525, 6125 |
| BstNI | CCWGG | 7 cut(s) | 581, 710, 1319, 3547, 4656, 5383, 5700 |
| BstNSI | RCATGY | 9 cut(s) | 1166, 2516, 2887, 3668, 4222, 4442, 4769, 4817, 5186 |
| BstPAI | GACNNNNGTC | 3 cut(s) | 760, 3051, 4094 |
| BstPI | GGTNACC | 2 cut(s) | 1817, 5831 |
| BstSFI | CTRYAG | 5 cut(s) | 606, 4893, 5523, 6414, 6495 |
| BstSLI | GKGCMC | 1 cut(s) | 1061 |
| BstSNI | TACGTA | 1 cut(s) | 2891 |
| BstV1I | GCAGC | 8 cut(s) | 19, 282, 409, 424, 2965, 3671, 3682, 4126 |
| BstV2I | GAAGAC | 7 cut(s) | 1086, 2367, 2677, 3362, 4090, 5639, 6437 |
| BstX2I | RGATCY | 5 cut(s) | 942, 2494, 4603, 4834, 5770 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 3497, 4401, 4484 |
| BstYI | RGATCY | 5 cut(s) | 942, 2494, 4603, 4834, 5770 |
| BstZ17I | GTATAC | 1 cut(s) | 6438 |
| BsuI | GTATCC | 3 cut(s) | 3993, 6300, 6325 |
| BtgI | CCRYGG | 3 cut(s) | 321, 3496, 4957 |
| BtgZI | GCGATG | 2 cut(s) | 4745, 5007 |
| BtrI | CACGTC | 1 cut(s) | 6079 |
| BtsI | GCAGTG | 8 cut(s) | 315, 615, 1647, 1667, 2532, 4749, 4949, 6347 |
| BveI | ACCTGC | 4 cut(s) | 271, 1221, 4084, 4438 |
| CaiI | CAGNNNCTG | 2 cut(s) | 6134, 6422 |
| CciI | TCATGA | 3 cut(s) | 1966, 3805, 5049 |
| CfoI | GCGC | 5 cut(s) | 37, 291, 1610, 4665, 4956 |
| Cfr10I | RCCGGY | 2 cut(s) | 291, 1038 |
| Cfr9I | CCCGGG | 1 cut(s) | 360 |
| CseI | GACGC | 3 cut(s) | 3356, 3487, 4099 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 4570, 4605 |
| DinI | GGCGCC | 1 cut(s) | 290 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 5187 |
| DriI | GACNNNNNGTC | 1 cut(s) | 5008 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 5187 |
| EaeI | YGGCCR | 2 cut(s) | 101, 5125 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 5008 |
| EciI | GGCGGA | 2 cut(s) | 4641, 4863 |
| Ecl136II | GAGCTC | 1 cut(s) | 3380 |
| Eco105I | TACGTA | 1 cut(s) | 2891 |
| Eco130I | CCWWGG | 8 cut(s) | 2341, 3496, 4257, 4599, 4957, 6284, 6300, 6387 |
| Eco147I | AGGCCT | 1 cut(s) | 4205 |
| Eco24I | GRGCYC | 5 cut(s) | 1516, 3264, 3324, 3382, 3415 |
| Eco31I | GGTCTC | 2 cut(s) | 350, 2018 |
| Eco32I | GATATC | 4 cut(s) | 876, 1888, 2218, 3514 |
| Eco47I | GGWCC | 9 cut(s) | 82, 706, 1153, 2827, 4298, 4499, 5385, 5845, 5857 |
| Eco53kI | GAGCTC | 1 cut(s) | 3380 |
| Eco72I | CACGTG | 1 cut(s) | 107 |
| Eco88I | CYCGRG | 2 cut(s) | 73, 360 |
| Eco91I | GGTNACC | 2 cut(s) | 1817, 5831 |
| EcoICRI | GAGCTC | 1 cut(s) | 3380 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 4245 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1979, 5857 |
| EcoO65I | GGTNACC | 2 cut(s) | 1817, 5831 |
| EcoRI | GAATTC | 1 cut(s) | 4560 |
| EcoRII | CCWGG | 7 cut(s) | 579, 708, 1317, 3545, 4654, 5381, 5698 |
| EcoRV | GATATC | 4 cut(s) | 876, 1888, 2218, 3514 |
| EcoT14I | CCWWGG | 8 cut(s) | 2341, 3496, 4257, 4599, 4957, 6284, 6300, 6387 |
| EcoT38I | GRGCYC | 5 cut(s) | 1516, 3264, 3324, 3382, 3415 |
| EgeI | GGCGCC | 1 cut(s) | 290 |
| EheI | GGCGCC | 1 cut(s) | 290 |
| ErhI | CCWWGG | 8 cut(s) | 2341, 3496, 4257, 4599, 4957, 6284, 6300, 6387 |
| Esp3I | CGTCTC | 2 cut(s) | 464, 1248 |
| FaqI | GGGAC | 5 cut(s) | 1274, 2132, 3143, 4485, 5193 |
| FauI | CCCGC | 2 cut(s) | 4826, 5844 |
| FauNDI | CATATG | 3 cut(s) | 2437, 2689, 4166 |
| FbaI | TGATCA | 3 cut(s) | 567, 4996, 5727 |
| FblI | GTMKAC | 4 cut(s) | 1363, 1566, 5012, 6437 |
| FriOI | GRGCYC | 5 cut(s) | 1516, 3264, 3324, 3382, 3415 |
| FspBI | CTAG | 7 cut(s) | 1176, 1269, 2004, 3063, 4524, 5837, 5936 |
| GlaI | GCGC | 5 cut(s) | 36, 290, 1609, 4664, 4955 |
| GsaI | CCCAGC | 4 cut(s) | 1419, 2244, 3366, 6225 |
| GsuI | CTGGAG | 1 cut(s) | 4377 |
| HaeII | RGCGCY | 1 cut(s) | 292 |
| HapII | CCGG | 8 cut(s) | 43, 134, 200, 292, 361, 1039, 1682, 5128 |
| HgaI | GACGC | 3 cut(s) | 3356, 3487, 4099 |
| HhaI | GCGC | 5 cut(s) | 37, 291, 1610, 4665, 4956 |
| Hin1I | GRCGYC | 3 cut(s) | 289, 3367, 3479 |
| Hin6I | GCGC | 5 cut(s) | 35, 289, 1608, 4663, 4954 |
| HinP1I | GCGC | 5 cut(s) | 35, 289, 1608, 4663, 4954 |
| HincII | GTYRAC | 2 cut(s) | 3454, 5013 |
| HindII | GTYRAC | 2 cut(s) | 3454, 5013 |
| HindIII | AAGCTT | 7 cut(s) | 215, 882, 920, 2844, 3311, 5198, 6395 |
| HpaI | GTTAAC | 1 cut(s) | 3454 |
| HpaII | CCGG | 8 cut(s) | 43, 134, 200, 292, 361, 1039, 1682, 5128 |
| HphI | GGTGA | 8 cut(s) | 185, 632, 968, 1829, 1885, 2245, 4525, 4562 |
| Hpy99I | CGWCG | 3 cut(s) | 1730, 3997, 4048 |
| HpyCH4IV | ACGT | 8 cut(s) | 106, 473, 1725, 2890, 4128, 4318, 6078, 6434 |
| HpySE526I | ACGT | 8 cut(s) | 106, 473, 1725, 2890, 4128, 4318, 6078, 6434 |
| Hsp92I | GRCGYC | 3 cut(s) | 289, 3367, 3479 |
| HspAI | GCGC | 5 cut(s) | 35, 289, 1608, 4663, 4954 |
| KasI | GGCGCC | 1 cut(s) | 288 |
| Kpn2I | TCCGGA | 1 cut(s) | 1681 |
| KroI | GCCGGC | 1 cut(s) | 291 |
| KroNI | GCCGGC | 1 cut(s) | 293 |
| Ksp22I | TGATCA | 3 cut(s) | 567, 4996, 5727 |
| KspAI | GTTAAC | 1 cut(s) | 3454 |
| LguI | GCTCTTC | 2 cut(s) | 1307, 3252 |
| Lsp1109I | GCAGC | 8 cut(s) | 19, 282, 409, 424, 2965, 3671, 3682, 4126 |
| MaeI | CTAG | 7 cut(s) | 1176, 1269, 2004, 3063, 4524, 5837, 5936 |
| MaeII | ACGT | 8 cut(s) | 106, 473, 1725, 2890, 4128, 4318, 6078, 6434 |
| MfeI | CAATTG | 5 cut(s) | 1146, 2204, 2483, 2798, 4755 |
| MflI | RGATCY | 5 cut(s) | 942, 2494, 4603, 4834, 5770 |
| MhlI | GDGCHC | 7 cut(s) | 315, 1061, 1516, 3264, 3324, 3382, 3415 |
| Mly113I | GGCGCC | 1 cut(s) | 289 |
| MmeI | TCCRAC | 5 cut(s) | 186, 2921, 3507, 3984, 4016 |
| MroI | TCCGGA | 1 cut(s) | 1681 |
| MroNI | GCCGGC | 1 cut(s) | 291 |
| MroXI | GAANNNNTTC | 6 cut(s) | 1049, 2382, 2814, 3882, 6105, 6117 |
| MslI | CAYNNNNRTG | 4 cut(s) | 910, 3747, 5039, 6182 |
| MspA1I | CMGCKG | 2 cut(s) | 3307, 5851 |
| MspCI | CTTAAG | 2 cut(s) | 218, 461 |
| MspI | CCGG | 8 cut(s) | 43, 134, 200, 292, 361, 1039, 1682, 5128 |
| MunI | CAATTG | 5 cut(s) | 1146, 2204, 2483, 2798, 4755 |
| Mva1269I | GAATGC | 3 cut(s) | 2809, 3603, 5276 |
| MvaI | CCWGG | 7 cut(s) | 581, 710, 1319, 3547, 4656, 5383, 5700 |
| NaeI | GCCGGC | 1 cut(s) | 293 |
| NarI | GGCGCC | 1 cut(s) | 289 |
| NciI | CCSGG | 4 cut(s) | 43, 361, 362, 5129 |
| NcoI | CCATGG | 2 cut(s) | 3496, 4957 |
| NdeI | CATATG | 3 cut(s) | 2437, 2689, 4166 |
| NgoMIV | GCCGGC | 1 cut(s) | 291 |
| NheI | GCTAGC | 1 cut(s) | 3062 |
| NlaIV | GGNNCC | 4 cut(s) | 290, 3756, 4501, 6411 |
| NmeAIII | GCCGAG | 2 cut(s) | 79, 4396 |
| NmuCI | GTSAC | 9 cut(s) | 107, 173, 620, 1795, 1817, 1864, 1930, 3178, 5981 |
| NspI | RCATGY | 9 cut(s) | 1166, 2516, 2887, 3668, 4222, 4442, 4769, 4817, 5186 |
| NspV | TTCGAA | 1 cut(s) | 5719 |
| PaeI | GCATGC | 2 cut(s) | 1166, 4222 |
| PaeR7I | CTCGAG | 1 cut(s) | 73 |
| PagI | TCATGA | 3 cut(s) | 1966, 3805, 5049 |
| PceI | AGGCCT | 1 cut(s) | 4205 |
| PciI | ACATGT | 2 cut(s) | 3664, 4438 |
| PciSI | GCTCTTC | 2 cut(s) | 1307, 3252 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 2311, 2887 |
| PctI | GAATGC | 3 cut(s) | 2809, 3603, 5276 |
| PdiI | GCCGGC | 1 cut(s) | 293 |
| PdmI | GAANNNNTTC | 6 cut(s) | 1049, 2382, 2814, 3882, 6105, 6117 |
| PflFI | GACNNNGTC | 2 cut(s) | 704, 4127 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 4963, 5095 |
| PfoI | TCCNGGA | 1 cut(s) | 708 |
| PluTI | GGCGCC | 1 cut(s) | 292 |
| PmaCI | CACGTG | 1 cut(s) | 107 |
| PmlI | CACGTG | 1 cut(s) | 107 |
| Ppu21I | YACGTR | 3 cut(s) | 107, 2891, 4319 |
| PpuMI | RGGWCCY | 1 cut(s) | 5857 |
| PscI | ACATGT | 2 cut(s) | 3664, 4438 |
| PshAI | GACNNNNGTC | 3 cut(s) | 760, 3051, 4094 |
| PshBI | ATTAAT | 1 cut(s) | 3276 |
| PsiI | TTATAA | 2 cut(s) | 1713, 5427 |
| Psp124BI | GAGCTC | 1 cut(s) | 3382 |
| Psp5II | RGGWCCY | 1 cut(s) | 5857 |
| Psp6I | CCWGG | 7 cut(s) | 579, 708, 1317, 3545, 4654, 5381, 5698 |
| PspCI | CACGTG | 1 cut(s) | 107 |
| PspEI | GGTNACC | 2 cut(s) | 1817, 5831 |
| PspFI | CCCAGC | 4 cut(s) | 1415, 2240, 3362, 6221 |
| PspGI | CCWGG | 7 cut(s) | 579, 708, 1317, 3545, 4654, 5381, 5698 |
| PspN4I | GGNNCC | 4 cut(s) | 290, 3756, 4501, 6411 |
| PspPPI | RGGWCCY | 1 cut(s) | 5857 |
| PspXI | VCTCGAGB | 1 cut(s) | 73 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 3079, 3111 |
| PstI | CTGCAG | 3 cut(s) | 610, 4897, 6418 |
| PstNI | CAGNNNCTG | 2 cut(s) | 6134, 6422 |
| PsuI | RGATCY | 5 cut(s) | 942, 2494, 4603, 4834, 5770 |
| PsyI | GACNNNGTC | 2 cut(s) | 704, 4127 |
| PvuII | CAGCTG | 1 cut(s) | 3307 |
| RseI | CAYNNNNRTG | 4 cut(s) | 910, 3747, 5039, 6182 |
| SacI | GAGCTC | 1 cut(s) | 3382 |
| SalI | GTCGAC | 1 cut(s) | 5011 |
| SapI | GCTCTTC | 2 cut(s) | 1307, 3252 |
| SduI | GDGCHC | 7 cut(s) | 315, 1061, 1516, 3264, 3324, 3382, 3415 |
| SfcI | CTRYAG | 5 cut(s) | 606, 4893, 5523, 6414, 6495 |
| SfoI | GGCGCC | 1 cut(s) | 290 |
| Sfr274I | CTCGAG | 1 cut(s) | 73 |
| SfuI | TTCGAA | 1 cut(s) | 5719 |
| SinI | GGWCC | 9 cut(s) | 82, 706, 1153, 2827, 4298, 4499, 5385, 5845, 5857 |
| SlaI | CTCGAG | 1 cut(s) | 73 |
| SmaI | CCCGGG | 1 cut(s) | 362 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 910, 3747, 5039, 6182 |
| SmlI | CTYRAG | 7 cut(s) | 73, 218, 461, 2278, 2929, 4364, 5651 |
| SmoI | CTYRAG | 7 cut(s) | 73, 218, 461, 2278, 2929, 4364, 5651 |
| SnaBI | TACGTA | 1 cut(s) | 2891 |
| SphI | GCATGC | 2 cut(s) | 1166, 4222 |
| SseBI | AGGCCT | 1 cut(s) | 4205 |
| SspDI | GGCGCC | 1 cut(s) | 288 |
| SspI | AATATT | 1 cut(s) | 5117 |
| SspMI | CTAG | 7 cut(s) | 1176, 1269, 2004, 3063, 4524, 5837, 5936 |
| SstI | GAGCTC | 1 cut(s) | 3382 |
| StuI | AGGCCT | 1 cut(s) | 4205 |
| StyI | CCWWGG | 8 cut(s) | 2341, 3496, 4257, 4599, 4957, 6284, 6300, 6387 |
| TaiI | ACGT | 8 cut(s) | 109, 476, 1728, 2893, 4131, 4321, 6081, 6437 |
| TatI | WGTACW | 5 cut(s) | 2515, 2849, 5031, 5394, 6502 |
| TauI | GCSGC | 4 cut(s) | 199, 368, 440, 6410 |
| TseFI | GTSAC | 9 cut(s) | 107, 173, 620, 1795, 1817, 1864, 1930, 3178, 5981 |
| TseI | GCWGC | 8 cut(s) | 7, 295, 397, 412, 2978, 3659, 3670, 4139 |
| Tsp45I | GTSAC | 9 cut(s) | 107, 173, 620, 1795, 1817, 1864, 1930, 3178, 5981 |
| TspGWI | ACGGA | 3 cut(s) | 338, 3059, 4010 |
| TspMI | CCCGGG | 1 cut(s) | 360 |
| Tth111I | GACNNNGTC | 2 cut(s) | 704, 4127 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 4963, 5095 |
| Vha464I | CTTAAG | 2 cut(s) | 218, 461 |
| VneI | GTGCAC | 1 cut(s) | 1057 |
| VpaK11BI | GGWCC | 9 cut(s) | 82, 706, 1153, 2827, 4298, 4499, 5385, 5845, 5857 |
| VspI | ATTAAT | 1 cut(s) | 3276 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 4245 |
| XapI | RAATTY | 6 cut(s) | 2379, 3167, 3870, 4039, 4560, 4708 |
| XceI | RCATGY | 9 cut(s) | 1166, 2516, 2887, 3668, 4222, 4442, 4769, 4817, 5186 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 76, 2539, 3723, 4484 |
| XhoI | CTCGAG | 1 cut(s) | 73 |
| XmaI | CCCGGG | 1 cut(s) | 360 |
| XmiI | GTMKAC | 4 cut(s) | 1363, 1566, 5012, 6437 |
| XmnI | GAANNNNTTC | 6 cut(s) | 1049, 2382, 2814, 3882, 6105, 6117 |
| XspI | CTAG | 7 cut(s) | 1176, 1269, 2004, 3063, 4524, 5837, 5936 |