RLG00000003218

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001534
Physical Location & Seq
Reverse (-)
45796579 .. 45797229
651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003218

Sequence Viewer

Length: 519 bp
ATGTTTAGGTTACTCTCTCTGAGAGGTGGGTGGAGGCCGTGGCCTCCTGGCGGCGGATCTGCAAATTGGACTGGAGGAGATGGGATTTCCCTCTCATCTGGTCGACGGGAGATTGAAGGTGGCGGGGTTCCTCGCTGTCAGCTTCTCAAAATTTTGCTTCTTGTTTTCTTGTTCGGGGTCGTCTGCTTTGGCAGTAGTGATGGTAATCCTGGGTGGCGCGAGGCAGTGGCGTTGGGATGTGATCCTAGGCTATTTGGATCGGTGACGGTCTCGGGTTCTCGACGCTTAGTGATGCCTGAGCAAGAAGATCTGGCGACTGCGATCGGCTGGGGTTGCGGTGGGCTGTTGGGTCAGATATTTTCGAGCGCCGTCATTGAAGGGAGGCAGATTGGTGGTTGGCGGTTGATGGATGGGGTGCCCAAGTGGATGGGTGGCCTCATCAATTCCAGTTGGGCTTGGTACTCTATTGGGCTTCCTTCTGATAGGGTTTCTTGTTTGGGTTTGGGCCTATTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

18.28

Weight (kDa)

8.77

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 415
AccI GTMKAC 1 cut(s) 103
AccII CGCG 1 cut(s) 219
AciI CCGC 5 cut(s) 51, 54, 123, 336, 400
AclWI GGATC 3 cut(s) 64, 236, 265
AcsI RAATTY 1 cut(s) 150
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 2 cut(s) 50, 53
AgsI TTSAA 2 cut(s) 116, 377
AjnI CCWGG 2 cut(s) 46, 208
AluBI AGCT 1 cut(s) 142
AluI AGCT 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 274
AlwI GGATC 3 cut(s) 64, 236, 265
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 4 cut(s) 35, 41, 433, 505
ApoI RAATTY 1 cut(s) 150
AspA2I CCTAGG 1 cut(s) 245
AspLEI GCGC 2 cut(s) 219, 368
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 1 cut(s) 274
AvaI CYCGRG 1 cut(s) 271
AvrII CCTAGG 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 420
BanI GGYRCC 1 cut(s) 415
BccI CCATC 5 cut(s) 74, 194, 400, 404, 421
BceAI ACGGC 2 cut(s) 22, 353
BciT130I CCWGG 2 cut(s) 48, 210
BcoDI GTCTC 1 cut(s) 274
BfaI CTAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 369
BglII AGATCT 1 cut(s) 307
BisI GCNGC 1 cut(s) 52
BlnI CCTAGG 1 cut(s) 245
BlsI GCNGC 1 cut(s) 53
Bme1390I CCNGG 2 cut(s) 48, 210
BmeT110I CYCGRG 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 2 cut(s) 129, 417
BmrFI CCNGG 2 cut(s) 48, 210
BmsI GCATC 1 cut(s) 282
BpmI CTGGAG 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 297
BsaBI GATNNNNATC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 274
BsaJI CCNNGG 3 cut(s) 38, 209, 245
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 53
Bse1I ACTGG 2 cut(s) 76, 447
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 2 cut(s) 48, 210
BseDI CCNNGG 3 cut(s) 38, 209, 245
BseGI GGATG 3 cut(s) 242, 415, 432
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 50, 53
BseMII CTCAG 2 cut(s) 11, 288
BseNI ACTGG 2 cut(s) 76, 447
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 420
BseYI CCCAGC 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 4 cut(s) 37, 43, 435, 507
BshNI GGYRCC 1 cut(s) 415
BsiEI CGRYCG 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 271
BslI CCNNNNNNNGG 2 cut(s) 50, 53
BsmAI GTCTC 1 cut(s) 274
BsnI GGCC 4 cut(s) 37, 43, 435, 507
Bso31I GGTCTC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 5 cut(s) 56, 241, 257, 307, 321
BspACI CCGC 5 cut(s) 51, 54, 123, 336, 400
BspANI GGCC 4 cut(s) 37, 43, 435, 507
BspCNI CTCAG 2 cut(s) 12, 289
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 129, 417
BspPI GGATC 3 cut(s) 64, 236, 265
BspT107I GGYRCC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 274
BsrI ACTGG 2 cut(s) 76, 447
BssECI CCNNGG 3 cut(s) 38, 209, 245
BssMI GATC 5 cut(s) 56, 241, 257, 307, 321
BssT1I CCWWGG 1 cut(s) 245
Bst2UI CCWGG 2 cut(s) 48, 210
Bst4CI ACNGT 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 20, 286, 297
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 242, 415, 432
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 2 cut(s) 219, 368
BstKTI GATC 5 cut(s) 59, 244, 260, 310, 324
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 5 cut(s) 56, 241, 257, 307, 321
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 1 cut(s) 333
BstNI CCWGG 2 cut(s) 48, 210
BstSCI CCNGG 2 cut(s) 46, 208
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 1 cut(s) 219
BstX2I RGATCY 2 cut(s) 56, 307
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 2 cut(s) 56, 307
BsuRI GGCC 4 cut(s) 37, 43, 435, 507
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 242, 415, 432
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
CfoI GCGC 2 cut(s) 219, 368
Cfr13I GGNCC 1 cut(s) 505
CseI GACGC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 460
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 3 cut(s) 20, 286, 297
DpnI GATC 5 cut(s) 58, 243, 259, 309, 323
DpnII GATC 5 cut(s) 56, 241, 257, 307, 321
EciI GGCGGA 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 245
Eco31I GGTCTC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 46, 208
EcoT14I CCWWGG 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 245
FauI CCCGC 1 cut(s) 116
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 3 cut(s) 249, 422, 439
Fsp4HI GCNGC 1 cut(s) 52
FspBI CTAG 1 cut(s) 246
GlaI GCGC 2 cut(s) 218, 367
GluI GCNGC 1 cut(s) 52
GsaI CCCAGC 1 cut(s) 331
GsuI CTGGAG 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 4 cut(s) 37, 43, 435, 507
HgaI GACGC 1 cut(s) 291
HhaI GCGC 2 cut(s) 219, 368
Hin6I GCGC 2 cut(s) 217, 366
HinP1I GCGC 2 cut(s) 217, 366
HincII GTYRAC 1 cut(s) 104
HindII GTYRAC 1 cut(s) 104
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 3 cut(s) 21, 354, 481
Hpy188III TCNNGA 1 cut(s) 279
Hpy8I GTNNAC 1 cut(s) 104
Hpy99I CGWCG 2 cut(s) 108, 285
HpyAV CCTTC 3 cut(s) 110, 371, 486
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 1 cut(s) 333
HpyF3I CTNAG 3 cut(s) 20, 286, 297
HspAI GCGC 2 cut(s) 217, 366
Kzo9I GATC 5 cut(s) 56, 241, 257, 307, 321
LweI GCATC 1 cut(s) 282
MaeI CTAG 1 cut(s) 246
MaeIII GTNAC 2 cut(s) 9, 262
MalI GATC 5 cut(s) 58, 243, 259, 309, 323
MboI GATC 5 cut(s) 56, 241, 257, 307, 321
MboII GAAGA 1 cut(s) 317
MflI RGATCY 2 cut(s) 56, 307
MhlI GDGCHC 1 cut(s) 420
MluCI AATT 3 cut(s) 64, 150, 442
MnlI CCTC 9 cut(s) 17, 27, 54, 68, 101, 141, 214, 375, 446
MspR9I CCNGG 2 cut(s) 48, 210
MvaI CCWGG 2 cut(s) 48, 210
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 333
NdeII GATC 5 cut(s) 56, 241, 257, 307, 321
NlaIV GGNNCC 2 cut(s) 129, 417
NmuCI GTSAC 1 cut(s) 262
PkrI GCNGC 1 cut(s) 53
Ple19I CGATCG 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 46, 208
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 2 cut(s) 46, 208
PspN4I GGNNCC 2 cut(s) 129, 417
PspPI GGNCC 1 cut(s) 505
PsuI RGATCY 2 cut(s) 56, 307
PvuI CGATCG 1 cut(s) 324
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
SalI GTCGAC 1 cut(s) 102
SatI GCNGC 1 cut(s) 52
Sau3AI GATC 5 cut(s) 56, 241, 257, 307, 321
Sau96I GGNCC 1 cut(s) 505
ScrFI CCNGG 2 cut(s) 48, 210
SduI GDGCHC 1 cut(s) 420
SetI ASST 4 cut(s) 11, 28, 121, 144
SfaNI GCATC 1 cut(s) 282
Sse9I AATT 3 cut(s) 64, 150, 442
SsiI CCGC 5 cut(s) 51, 54, 123, 336, 400
SspMI CTAG 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 46, 208
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 3 cut(s) 103, 280, 362
TasI AATT 3 cut(s) 64, 150, 442
TauI GCSGC 1 cut(s) 54
TscAI CASTG 1 cut(s) 231
TseFI GTSAC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 262
TspRI CASTG 1 cut(s) 231
XapI RAATTY 1 cut(s) 150
XmaJI CCTAGG 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 103
XspI CTAG 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.