RLG00000028471

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001534
Physical Location & Seq
Reverse (-)
23221407 .. 23221862
456 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028471

Sequence Viewer

Length: 456 bp
ATGTCTAGGTTACTCTCTCTGAGAGGTAGGTGGAGGCCGTGGCCTCCTGGCGGCGGTTCTGTAAATTGGGTTAGAGGAGATGGGGTTTCCTTCTCGTCTAGTCGCTGGGGGATTGAGGATGGCGGGGTTCCCCGTCGTCGGATTCTCAAAATTGGGGGCAATGAAGAGCTTCTGTCTCGGGTGGCTTTTCACTGCAGCTGCAATGGTTTCGAGTCTTTCTTGACCCGGGATGTTTTGGAAGGATTGGCTCGTCGGGTCGAGGAGTGGAGCACCAGGGGGAGGTCTGCCTCCTCGGGATTTCCTTTTGTGCCAGGCGGCTGGGCGGGGATGGTGGCTGATGACTGGCCGCCGTACAAGGAGGCTTGCACGGCCGGGCTAACAGATGCAGCGCTGATCCGTGCTAGAGCGGGGTTGGTTGCTGCTGGGAGAGGTGACGATGGCGACGACCAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.42

Weight (kDa)

8.63

Isoelectric Point (pI)

65.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 407
AciI CCGC 7 cut(s) 51, 54, 123, 315, 323, 347, 407
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 2 cut(s) 344, 369
AfaI GTAC 1 cut(s) 353
AfeI AGCGCT 1 cut(s) 390
AfiI CCNNNNNNNGG 4 cut(s) 50, 53, 138, 279
AjnI CCWGG 3 cut(s) 46, 272, 310
AluBI AGCT 2 cut(s) 169, 198
AluI AGCT 2 cut(s) 169, 198
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 388
Ama87I CYCGRG 3 cut(s) 177, 225, 292
Aor51HI AGCGCT 1 cut(s) 390
AoxI GGCC 4 cut(s) 35, 41, 344, 369
ApeKI GCWGC 4 cut(s) 195, 198, 386, 419
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 391
AsuC2I CCSGG 3 cut(s) 226, 227, 373
AsuHPI GGTGA 1 cut(s) 443
AvaI CYCGRG 3 cut(s) 177, 225, 292
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 4 cut(s) 185, 207, 398, 406
BccI CCATC 4 cut(s) 74, 113, 322, 431
BceAI ACGGC 3 cut(s) 22, 334, 384
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 3 cut(s) 48, 274, 312
BcnI CCSGG 3 cut(s) 226, 227, 373
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 3 cut(s) 6, 99, 402
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 392
BisI GCNGC 7 cut(s) 52, 196, 199, 316, 347, 387, 420
BlsI GCNGC 7 cut(s) 53, 197, 200, 317, 348, 388, 421
Bme1390I CCNGG 6 cut(s) 48, 226, 227, 274, 312, 373
BmeT110I CYCGRG 3 cut(s) 177, 225, 292
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 6 cut(s) 48, 226, 227, 274, 312, 373
BmsI GCATC 1 cut(s) 373
BpuMI CCSGG 3 cut(s) 226, 227, 373
BsaJI CCNNGG 4 cut(s) 38, 225, 273, 291
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 53, 138, 279
Bse1I ACTGG 2 cut(s) 347, 448
Bse3DI GCAATG 2 cut(s) 166, 208
BseBI CCWGG 3 cut(s) 48, 274, 312
BseDI CCNNGG 4 cut(s) 38, 225, 273, 291
BseGI GGATG 3 cut(s) 124, 235, 333
BseLI CCNNNNNNNGG 4 cut(s) 50, 53, 138, 279
BseMI GCAATG 2 cut(s) 166, 208
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 2 cut(s) 347, 448
BseRI GAGGAG 3 cut(s) 90, 275, 280
BseX3I CGGCCG 1 cut(s) 369
BseXI GCAGC 4 cut(s) 185, 207, 398, 406
BseYI CCCAGC 3 cut(s) 105, 318, 422
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 4 cut(s) 37, 43, 346, 371
BsiEI CGRYCG 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 272
BsiHKCI CYCGRG 3 cut(s) 177, 225, 292
BsiSI CCGG 2 cut(s) 226, 372
BslI CCNNNNNNNGG 4 cut(s) 50, 53, 138, 279
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 4 cut(s) 37, 43, 346, 371
BsoBI CYCGRG 3 cut(s) 177, 225, 292
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 1 cut(s) 393
BspACI CCGC 7 cut(s) 51, 54, 123, 315, 323, 347, 407
BspANI GGCC 4 cut(s) 37, 43, 346, 371
BspCNI CTCAG 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 129
BspMAI CTGCAG 1 cut(s) 197
BspPI GGATC 1 cut(s) 388
BspQI GCTCTTC 1 cut(s) 159
BsrBI CCGCTC 1 cut(s) 407
BsrDI GCAATG 2 cut(s) 166, 208
BsrI ACTGG 2 cut(s) 347, 448
BssECI CCNNGG 4 cut(s) 38, 225, 273, 291
BssMI GATC 1 cut(s) 393
Bst2UI CCWGG 3 cut(s) 48, 274, 312
Bst6I CTCTTC 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 1 cut(s) 20
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 124, 235, 333
BstH2I RGCGCY 1 cut(s) 392
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 1 cut(s) 393
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 3 cut(s) 48, 274, 312
BstSCI CCNGG 6 cut(s) 46, 224, 225, 272, 310, 371
BstSFI CTRYAG 1 cut(s) 193
BstV1I GCAGC 4 cut(s) 185, 207, 398, 406
BstXI CCANNNNNNTGG 1 cut(s) 318
BstZI CGGCCG 1 cut(s) 369
BsuRI GGCC 4 cut(s) 37, 43, 346, 371
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 124, 235, 333
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 2 cut(s) 190, 455
Cac8I GCNNGC 1 cut(s) 364
CfoI GCGC 1 cut(s) 391
Cfr9I CCCGGG 1 cut(s) 225
Csp6I GTAC 1 cut(s) 352
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 1 cut(s) 20
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
EaeI YGGCCR 2 cut(s) 344, 369
EagI CGGCCG 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EclXI CGGCCG 1 cut(s) 369
Eco47III AGCGCT 1 cut(s) 390
Eco52I CGGCCG 1 cut(s) 369
Eco88I CYCGRG 3 cut(s) 177, 225, 292
EcoRII CCWGG 3 cut(s) 46, 272, 310
FauI CCCGC 3 cut(s) 116, 316, 400
Fnu4HI GCNGC 7 cut(s) 52, 196, 199, 316, 347, 387, 420
FokI GGATG 3 cut(s) 131, 242, 340
Fsp4HI GCNGC 7 cut(s) 52, 196, 199, 316, 347, 387, 420
FspBI CTAG 3 cut(s) 6, 99, 402
GlaI GCGC 1 cut(s) 390
GluI GCNGC 7 cut(s) 52, 196, 199, 316, 347, 387, 420
GsaI CCCAGC 3 cut(s) 109, 322, 426
HaeII RGCGCY 1 cut(s) 392
HaeIII GGCC 4 cut(s) 37, 43, 346, 371
HapII CCGG 2 cut(s) 226, 372
HhaI GCGC 1 cut(s) 391
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HinfI GANTC 2 cut(s) 142, 212
HpaII CCGG 2 cut(s) 226, 372
HphI GGTGA 1 cut(s) 443
Hpy188I TCNGA 2 cut(s) 21, 141
Hpy188III TCNNGA 2 cut(s) 220, 294
Hpy99I CGWCG 4 cut(s) 138, 141, 255, 446
HpyAV CCTTC 2 cut(s) 100, 233
HpyCH4V TGCA 4 cut(s) 195, 201, 366, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 1 cut(s) 20
HspAI GCGC 1 cut(s) 389
Kzo9I GATC 1 cut(s) 393
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 4 cut(s) 185, 207, 398, 406
LweI GCATC 1 cut(s) 373
MaeI CTAG 3 cut(s) 6, 99, 402
MaeIII GTNAC 2 cut(s) 9, 431
MalI GATC 1 cut(s) 395
MbiI CCGCTC 1 cut(s) 407
MboI GATC 1 cut(s) 393
MboII GAAGA 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 2 cut(s) 64, 150
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 119
MroXI GAANNNNTTC 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 2 cut(s) 226, 372
MspR9I CCNGG 6 cut(s) 48, 226, 227, 274, 312, 373
MvaI CCWGG 3 cut(s) 48, 274, 312
MwoI GCNNNNNNNGC 1 cut(s) 368
NciI CCSGG 3 cut(s) 226, 227, 373
NdeII GATC 1 cut(s) 393
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 1 cut(s) 142
PkrI GCNGC 7 cut(s) 53, 197, 200, 317, 348, 388, 421
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 46, 272, 310
PspFI CCCAGC 3 cut(s) 105, 318, 422
PspGI CCWGG 3 cut(s) 46, 272, 310
PspN4I GGNNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 198
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SapI GCTCTTC 1 cut(s) 159
SatI GCNGC 7 cut(s) 52, 196, 199, 316, 347, 387, 420
Sau3AI GATC 1 cut(s) 393
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 6 cut(s) 48, 226, 227, 274, 312, 373
SduI GDGCHC 1 cut(s) 272
SetI ASST 7 cut(s) 11, 28, 32, 171, 200, 284, 433
SfaNI GCATC 1 cut(s) 373
SfcI CTRYAG 1 cut(s) 193
SmaI CCCGGG 1 cut(s) 227
Sse9I AATT 2 cut(s) 64, 150
SsiI CCGC 7 cut(s) 51, 54, 123, 315, 323, 347, 407
SspMI CTAG 3 cut(s) 6, 99, 402
StyD4I CCNGG 6 cut(s) 46, 224, 225, 272, 310, 371
TaqI TCGA 2 cut(s) 210, 258
TasI AATT 2 cut(s) 64, 150
TauI GCSGC 3 cut(s) 54, 318, 349
TfiI GAWTC 1 cut(s) 142
TscAI CASTG 2 cut(s) 197, 455
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 4 cut(s) 195, 198, 386, 419
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 386
TspMI CCCGGG 1 cut(s) 225
TspRI CASTG 2 cut(s) 197, 455
XmaI CCCGGG 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 3 cut(s) 6, 99, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.