RLG00000005735

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001534
Physical Location & Seq
Reverse (-)
1322213 .. 1322845
633 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005735

Sequence Viewer

Length: 552 bp
ATGCTTAGGTTACTCTCTCTGAGAGGTGGGTGGAGGTCGTGGCCTCTTGGCGGCGGCTCTACAAATTGGGCTAGAGGAAATGGGATTTCCCTCTCATCTGGTCGACGGGGGATTGAAGGCGGCGGGGATGCTCGTTGTCGGCTTCTCAAAATTTTGCTTCTTGTTTTCTTGTTCGGGGTTGTGTGCTTTGGCAGCAGTGATGGTAATTTTGGACCGCACAAGGAAGATCTGGTGATCGCGGCTGGTCGAGGTACGGACTGGAGCTTCGGGTGGTGGCCTGGGGTGGATTCTAGACCTCCATTGCTGGCGGTCGAGGATTGTGACAGACGTGGCGACGTCAATTACCTTGGACTAGGGTTGCCTGCGGCTTGGTGCGGCGACAGTTATGGTGGACTGCTGGGTCAGTTGTCGACCGGTGACGTTGAAGGGAGGAAGACTAGTGGTTGGCGGCTGATGGCTGGGGTGCCCAAGTGGATGGGTGGCCTTATCAATTCCAGCTGGGCTTGGTGCTCTTTGGGGCTTCCATCTGCTAGGGTTTCTTTTTGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

19.61

Weight (kDa)

9.22

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 399
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 2 cut(s) 103, 410
AccII CGCG 1 cut(s) 239
AcsI RAATTY 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 253
AfiI CCNNNNNNNGG 1 cut(s) 50
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 2 cut(s) 116, 425
AhlI ACTAGT 1 cut(s) 437
AjiI CACGTC 1 cut(s) 329
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 2 cut(s) 264, 498
AluI AGCT 2 cut(s) 264, 498
Alw21I GWGCWC 1 cut(s) 512
AoxI GGCC 3 cut(s) 41, 275, 481
ApeKI GCWGC 1 cut(s) 192
ApoI RAATTY 1 cut(s) 150
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AsiGI ACCGGT 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 244, 428
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 468
BanI GGYRCC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 440
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 1 cut(s) 204
BccI CCATC 4 cut(s) 194, 448, 469, 532
BciT130I CCWGG 1 cut(s) 279
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 6 cut(s) 72, 291, 353, 438, 531, 550
BglII AGATCT 1 cut(s) 226
BisI GCNGC 8 cut(s) 52, 55, 121, 193, 240, 366, 376, 449
BlsI GCNGC 8 cut(s) 53, 56, 122, 194, 241, 367, 377, 450
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 212
BmgBI CACGTC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 440
BpmI CTGGAG 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 5
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 278, 346
BsaWI WCCGGW 1 cut(s) 413
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 278, 346
BseGI GGATG 2 cut(s) 133, 480
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 1 cut(s) 204
BseYI CCCAGC 3 cut(s) 397, 458, 498
Bsh1236I CGCG 1 cut(s) 239
Bsh1285I CGRYCG 2 cut(s) 312, 414
BshFI GGCC 3 cut(s) 43, 277, 483
BshNI GGYRCC 1 cut(s) 463
BshTI ACCGGT 1 cut(s) 413
BsiEI CGRYCG 2 cut(s) 312, 414
BsiHKAI GWGCWC 1 cut(s) 512
BsiSI CCGG 1 cut(s) 414
BslI CCNNNNNNNGG 1 cut(s) 50
BsnI GGCC 3 cut(s) 43, 277, 483
Bsp1286I GDGCHC 2 cut(s) 468, 512
Bsp143I GATC 2 cut(s) 226, 234
BspANI GGCC 3 cut(s) 43, 277, 483
BspCNI CTCAG 1 cut(s) 12
BspFNI CGCG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 299
BsrFI RCCGGY 1 cut(s) 413
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 278, 346
BssMI GATC 2 cut(s) 226, 234
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 1 cut(s) 383
BstACI GRCGYC 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 306, 363
BstDEI CTNAG 2 cut(s) 5, 20
BstF5I GGATG 2 cut(s) 133, 480
BstFNI CGCG 1 cut(s) 239
BstKTI GATC 2 cut(s) 229, 237
BstMBI GATC 2 cut(s) 226, 234
BstMCI CGRYCG 2 cut(s) 312, 414
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 204
BstV2I GAAGAC 1 cut(s) 440
BstX2I RGATCY 1 cut(s) 226
BstXI CCANNNNNNTGG 1 cut(s) 475
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 3 cut(s) 43, 277, 483
BtrI CACGTC 1 cut(s) 329
BtsCI GGATG 2 cut(s) 133, 480
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 2 cut(s) 306, 363
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 252
CspAI ACCGGT 1 cut(s) 413
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 2 cut(s) 5, 20
DpnI GATC 2 cut(s) 228, 236
DpnII GATC 2 cut(s) 226, 234
DrdI GACNNNNNNGTC 1 cut(s) 399
DseDI GACNNNNNNGTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 346
FaiI YATR 1 cut(s) 387
FauI CCCGC 1 cut(s) 116
FblI GTMKAC 2 cut(s) 103, 410
Fnu4HI GCNGC 8 cut(s) 52, 55, 121, 193, 240, 366, 376, 449
FokI GGATG 2 cut(s) 140, 487
Fsp4HI GCNGC 8 cut(s) 52, 55, 121, 193, 240, 366, 376, 449
FspBI CTAG 6 cut(s) 72, 291, 353, 438, 531, 550
GluI GCNGC 8 cut(s) 52, 55, 121, 193, 240, 366, 376, 449
GsaI CCCAGC 3 cut(s) 401, 462, 502
GsuI CTGGAG 1 cut(s) 280
HaeIII GGCC 3 cut(s) 43, 277, 483
HapII CCGG 1 cut(s) 414
Hin1I GRCGYC 1 cut(s) 336
HincII GTYRAC 2 cut(s) 104, 411
HindII GTYRAC 2 cut(s) 104, 411
HinfI GANTC 1 cut(s) 287
HpaII CCGG 1 cut(s) 414
HphI GGTGA 2 cut(s) 244, 428
Hpy166II GTNNAC 3 cut(s) 104, 392, 411
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 1 cut(s) 291
Hpy8I GTNNAC 3 cut(s) 104, 392, 411
Hpy99I CGWCG 2 cut(s) 108, 338
HpyAV CCTTC 2 cut(s) 110, 419
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4IV ACGT 3 cut(s) 328, 336, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 2 cut(s) 5, 20
HpySE526I ACGT 3 cut(s) 328, 336, 420
Hsp92I GRCGYC 1 cut(s) 336
Kzo9I GATC 2 cut(s) 226, 234
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 1 cut(s) 118
MaeI CTAG 6 cut(s) 72, 291, 353, 438, 531, 550
MaeII ACGT 3 cut(s) 328, 336, 420
MaeIII GTNAC 3 cut(s) 9, 320, 416
MalI GATC 2 cut(s) 228, 236
MboI GATC 2 cut(s) 226, 234
MboII GAAGA 2 cut(s) 236, 445
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 2 cut(s) 468, 512
MluCI AATT 5 cut(s) 64, 150, 205, 340, 490
MnlI CCTC 9 cut(s) 17, 27, 54, 68, 101, 242, 306, 307, 423
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 192
NdeII GATC 2 cut(s) 226, 234
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 2 cut(s) 320, 416
PfeI GAWTC 1 cut(s) 287
PinAI ACCGGT 1 cut(s) 413
PkrI GCNGC 8 cut(s) 53, 56, 122, 194, 241, 367, 377, 450
Psp6I CCWGG 1 cut(s) 277
PspFI CCCAGC 3 cut(s) 397, 458, 498
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 212
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 498
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
SalI GTCGAC 2 cut(s) 102, 409
SatI GCNGC 8 cut(s) 52, 55, 121, 193, 240, 366, 376, 449
Sau3AI GATC 2 cut(s) 226, 234
Sau96I GGNCC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 468, 512
SfaNI GCATC 1 cut(s) 118
SinI GGWCC 1 cut(s) 212
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 5 cut(s) 64, 150, 205, 340, 490
SspMI CTAG 6 cut(s) 72, 291, 353, 438, 531, 550
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 383
TaiI ACGT 3 cut(s) 331, 339, 423
TaqI TCGA 4 cut(s) 103, 247, 312, 410
TasI AATT 5 cut(s) 64, 150, 205, 340, 490
TauI GCSGC 7 cut(s) 54, 57, 123, 242, 368, 378, 451
TfiI GAWTC 1 cut(s) 287
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 320, 416
TseI GCWGC 1 cut(s) 192
Tsp45I GTSAC 2 cut(s) 320, 416
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 150
XbaI TCTAGA 1 cut(s) 290
XmiI GTMKAC 2 cut(s) 103, 410
XspI CTAG 6 cut(s) 72, 291, 353, 438, 531, 550
ZraI GACGTC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.