RLG00000025131

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001534
Physical Location & Seq
Reverse (-)
42289081 .. 42289555
475 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025131

Sequence Viewer

Length: 375 bp
ATGTTGCTTCTTGTTTTCTTGTTTGAGGTCGTCTGCTTTGGCAGCCGCGATGGAAGATCTAGTGGCCGTGGCAGGTTGGGGTGTGGACTGGAGCTCAGAGCGGTGGCCTGGGGTGGGCTCTTGACTTCTGTTGATGGCGGTCGAGGATTGAGATGGAGATGGCGATGTCAACCACCTTGGACTGGGGTTAACTGCGGCGAAGATTGCGGTGGGCTGCTGGGTCAGATCTCTTCGACCGACGTCTTTGGAGGGAGGCAGACTAGTGGTTGGCGGCTGAGGGATGGGTGCCCAAGTGGTTGGGTGGCCTCATCAATTCCAGCTGGGCTGGGTACTCTGTTGGGCTTCCATCTGCTAGGGTTTCTTGTTTGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.28

Weight (kDa)

9.0

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 285
AccBSI CCGCTC 1 cut(s) 101
AccII CGCG 1 cut(s) 48
AciI CCGC 6 cut(s) 46, 101, 138, 195, 207, 271
AcoI YGGCCR 1 cut(s) 64
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 2 cut(s) 114, 182
AhlI ACTAGT 1 cut(s) 260
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 2 cut(s) 94, 320
AluI AGCT 2 cut(s) 94, 320
Alw21I GWGCWC 1 cut(s) 96
AoxI GGCC 3 cut(s) 64, 105, 303
ApeKI GCWGC 2 cut(s) 42, 214
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 285
BanII GRGCYC 2 cut(s) 96, 120
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 275
BbvI GCAGC 2 cut(s) 54, 201
BccI CCATC 6 cut(s) 44, 128, 147, 153, 275, 354
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 1 cut(s) 109
BcuI ACTAGT 1 cut(s) 260
BfaI CTAG 3 cut(s) 60, 261, 353
BfuAI ACCTGC 1 cut(s) 63
BglII AGATCT 2 cut(s) 56, 225
BisI GCNGC 5 cut(s) 43, 46, 196, 215, 272
BlsI GCNGC 5 cut(s) 44, 47, 197, 216, 273
Bme1390I CCNGG 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 109
BmrI ACTGGG 1 cut(s) 192
BmuI ACTGGG 1 cut(s) 192
BoxI GACNNNNGTC 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 275
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 67, 108, 176
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 182
Bse1I ACTGG 2 cut(s) 93, 187
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 3 cut(s) 67, 108, 176
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 114, 182
BseMII CTCAG 2 cut(s) 109, 266
BseNI ACTGG 2 cut(s) 93, 187
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 2 cut(s) 54, 201
BseYI CCCAGC 3 cut(s) 217, 320, 325
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 2 cut(s) 142, 237
BshFI GGCC 3 cut(s) 66, 107, 305
BshNI GGYRCC 1 cut(s) 285
BsiEI CGRYCG 2 cut(s) 142, 237
BsiHKAI GWGCWC 1 cut(s) 96
BslI CCNNNNNNNGG 2 cut(s) 114, 182
BsnI GGCC 3 cut(s) 66, 107, 305
Bsp1286I GDGCHC 3 cut(s) 96, 120, 290
Bsp143I GATC 3 cut(s) 56, 225, 369
BspACI CCGC 6 cut(s) 46, 101, 138, 195, 207, 271
BspANI GGCC 3 cut(s) 66, 107, 305
BspCNI CTCAG 2 cut(s) 108, 267
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 287
BspMI ACCTGC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 101
BsrI ACTGG 2 cut(s) 93, 187
BssECI CCNNGG 3 cut(s) 67, 108, 176
BssMI GATC 3 cut(s) 56, 225, 369
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 109
Bst6I CTCTTC 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 240
BstDEI CTNAG 2 cut(s) 95, 275
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 1 cut(s) 286
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 3 cut(s) 59, 228, 372
BstMBI GATC 3 cut(s) 56, 225, 369
BstMCI CGRYCG 2 cut(s) 142, 237
BstMWI GCNNNNNNNGC 2 cut(s) 42, 204
BstNI CCWGG 1 cut(s) 109
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 107
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 2 cut(s) 54, 201
BstX2I RGATCY 3 cut(s) 56, 225, 369
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 3 cut(s) 56, 225, 369
BsuRI GGCC 3 cut(s) 66, 107, 305
BtgI CCRYGG 1 cut(s) 67
BtgZI GCGATG 2 cut(s) 63, 178
BtsCI GGATG 1 cut(s) 286
BveI ACCTGC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 330
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 95, 275
DpnI GATC 3 cut(s) 58, 227, 371
DpnII GATC 3 cut(s) 56, 225, 369
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 176
Eco24I GRGCYC 2 cut(s) 96, 120
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 176
EcoT38I GRGCYC 2 cut(s) 96, 120
ErhI CCWWGG 1 cut(s) 176
Fnu4HI GCNGC 5 cut(s) 43, 46, 196, 215, 272
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 2 cut(s) 96, 120
Fsp4HI GCNGC 5 cut(s) 43, 46, 196, 215, 272
FspBI CTAG 3 cut(s) 60, 261, 353
GluI GCNGC 5 cut(s) 43, 46, 196, 215, 272
GsaI CCCAGC 3 cut(s) 221, 324, 329
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 3 cut(s) 66, 107, 305
Hin1I GRCGYC 1 cut(s) 240
HincII GTYRAC 2 cut(s) 170, 190
HindII GTYRAC 2 cut(s) 170, 190
HpaI GTTAAC 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 86, 170, 190
Hpy188I TCNGA 3 cut(s) 98, 225, 374
Hpy188III TCNNGA 1 cut(s) 121
Hpy8I GTNNAC 3 cut(s) 86, 170, 190
Hpy99I CGWCG 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 204
HpyF3I CTNAG 2 cut(s) 95, 275
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 240
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 56, 225, 369
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 9 cut(s) 58, 74, 94, 121, 168, 203, 306, 311, 330
Lsp1109I GCAGC 2 cut(s) 54, 201
MaeI CTAG 3 cut(s) 60, 261, 353
MaeII ACGT 1 cut(s) 240
MalI GATC 3 cut(s) 58, 227, 371
MbiI CCGCTC 1 cut(s) 101
MboI GATC 3 cut(s) 56, 225, 369
MboII GAAGA 3 cut(s) 66, 212, 222
MflI RGATCY 3 cut(s) 56, 225, 369
MhlI GDGCHC 3 cut(s) 96, 120, 290
MluCI AATT 1 cut(s) 312
MnlI CCTC 6 cut(s) 19, 137, 242, 246, 270, 316
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 109
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 2 cut(s) 42, 204
NdeII GATC 3 cut(s) 56, 225, 369
NlaIV GGNNCC 1 cut(s) 287
PkrI GCNGC 5 cut(s) 44, 47, 197, 216, 273
PshAI GACNNNNGTC 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 107
PspFI CCCAGC 3 cut(s) 217, 320, 325
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 287
PsuI RGATCY 3 cut(s) 56, 225, 369
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
SacI GAGCTC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 5 cut(s) 43, 46, 196, 215, 272
Sau3AI GATC 3 cut(s) 56, 225, 369
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 3 cut(s) 96, 120, 290
SetI ASST 6 cut(s) 30, 77, 96, 178, 243, 322
SpeI ACTAGT 1 cut(s) 260
Sse9I AATT 1 cut(s) 312
SsiI CCGC 6 cut(s) 46, 101, 138, 195, 207, 271
SspMI CTAG 3 cut(s) 60, 261, 353
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 1 cut(s) 176
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 142, 233
TaqII GACCGA 1 cut(s) 251
TasI AATT 1 cut(s) 312
TauI GCSGC 3 cut(s) 48, 198, 274
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 2 cut(s) 42, 214
XspI CTAG 3 cut(s) 60, 261, 353
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.