Mediates both low-affinity uptake and efflux of sugar across the membrane
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 341, 406 |
| AclWI | GGATC | 1 cut(s) | 122 |
| AcoI | YGGCCR | 1 cut(s) | 531 |
| AcsI | RAATTY | 1 cut(s) | 326 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 290 |
| AgsI | TTSAA | 2 cut(s) | 442, 638 |
| AluBI | AGCT | 5 cut(s) | 14, 122, 233, 250, 459 |
| AluI | AGCT | 5 cut(s) | 14, 122, 233, 250, 459 |
| Alw26I | GTCTC | 1 cut(s) | 617 |
| AlwI | GGATC | 1 cut(s) | 122 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 17 |
| AoxI | GGCC | 1 cut(s) | 531 |
| ApoI | RAATTY | 1 cut(s) | 326 |
| AseI | ATTAAT | 1 cut(s) | 225 |
| AsuC2I | CCSGG | 1 cut(s) | 61 |
| AsuHPI | GGTGA | 1 cut(s) | 335 |
| BaeGI | GKGCMC | 1 cut(s) | 9 |
| BalI | TGGCCA | 1 cut(s) | 533 |
| BbsI | GAAGAC | 1 cut(s) | 687 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 595, 629 |
| BcnI | CCSGG | 1 cut(s) | 61 |
| BcoDI | GTCTC | 1 cut(s) | 617 |
| BfaI | CTAG | 3 cut(s) | 21, 239, 690 |
| BfmI | CTRYAG | 1 cut(s) | 147 |
| BisI | GCNGC | 1 cut(s) | 342 |
| BlsI | GCNGC | 1 cut(s) | 343 |
| Bme1390I | CCNGG | 1 cut(s) | 61 |
| BmrFI | CCNGG | 1 cut(s) | 61 |
| BmrI | ACTGGG | 1 cut(s) | 71 |
| BmuI | ACTGGG | 1 cut(s) | 71 |
| BpiI | GAAGAC | 1 cut(s) | 687 |
| BpmI | CTGGAG | 2 cut(s) | 267, 648 |
| BpuMI | CCSGG | 1 cut(s) | 61 |
| BsaI | GGTCTC | 1 cut(s) | 617 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 290 |
| Bse1I | ACTGG | 1 cut(s) | 77 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 290 |
| BseMII | CTCAG | 1 cut(s) | 366 |
| BseNI | ACTGG | 1 cut(s) | 77 |
| BseRI | GAGGAG | 1 cut(s) | 453 |
| BseSI | GKGCMC | 1 cut(s) | 9 |
| BseYI | CCCAGC | 1 cut(s) | 233 |
| BshFI | GGCC | 1 cut(s) | 533 |
| BsiSI | CCGG | 1 cut(s) | 60 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 290 |
| BsmAI | GTCTC | 1 cut(s) | 617 |
| BsmI | GAATGC | 1 cut(s) | 189 |
| BsnI | GGCC | 1 cut(s) | 533 |
| Bso31I | GGTCTC | 1 cut(s) | 617 |
| Bsp1286I | GDGCHC | 1 cut(s) | 9 |
| Bsp143I | GATC | 1 cut(s) | 127 |
| BspACI | CCGC | 2 cut(s) | 341, 406 |
| BspANI | GGCC | 1 cut(s) | 533 |
| BspCNI | CTCAG | 1 cut(s) | 367 |
| BspPI | GGATC | 1 cut(s) | 122 |
| BspTNI | GGTCTC | 1 cut(s) | 617 |
| BsrI | ACTGG | 1 cut(s) | 77 |
| BssMI | GATC | 1 cut(s) | 127 |
| Bst4CI | ACNGT | 4 cut(s) | 151, 161, 206, 611 |
| Bst6I | CTCTTC | 1 cut(s) | 491 |
| BstC8I | GCNNGC | 2 cut(s) | 408, 695 |
| BstDEI | CTNAG | 2 cut(s) | 316, 375 |
| BstEII | GGTNACC | 1 cut(s) | 304 |
| BstKTI | GATC | 1 cut(s) | 130 |
| BstMAI | GTCTC | 1 cut(s) | 617 |
| BstMBI | GATC | 1 cut(s) | 127 |
| BstNSI | RCATGY | 1 cut(s) | 514 |
| BstPI | GGTNACC | 1 cut(s) | 304 |
| BstSCI | CCNGG | 1 cut(s) | 59 |
| BstSFI | CTRYAG | 1 cut(s) | 147 |
| BstSLI | GKGCMC | 1 cut(s) | 9 |
| BstV2I | GAAGAC | 1 cut(s) | 687 |
| BsuRI | GGCC | 1 cut(s) | 533 |
| BtsIMutI | CAGTG | 1 cut(s) | 156 |
| Cac8I | GCNNGC | 2 cut(s) | 408, 695 |
| CaiI | CAGNNNCTG | 1 cut(s) | 17 |
| CviAII | CATG | 3 cut(s) | 28, 451, 511 |
| DdeI | CTNAG | 2 cut(s) | 316, 375 |
| DpnI | GATC | 1 cut(s) | 129 |
| DpnII | GATC | 1 cut(s) | 127 |
| EaeI | YGGCCR | 1 cut(s) | 531 |
| Eam1104I | CTCTTC | 1 cut(s) | 491 |
| EarI | CTCTTC | 1 cut(s) | 491 |
| Eco31I | GGTCTC | 1 cut(s) | 617 |
| Eco91I | GGTNACC | 1 cut(s) | 304 |
| EcoO65I | GGTNACC | 1 cut(s) | 304 |
| FaeI | CATG | 3 cut(s) | 31, 454, 514 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 699, 731 |
| FatI | CATG | 3 cut(s) | 27, 450, 510 |
| FauI | CCCGC | 1 cut(s) | 399 |
| FauNDI | CATATG | 1 cut(s) | 565 |
| Fnu4HI | GCNGC | 1 cut(s) | 342 |
| Fsp4HI | GCNGC | 1 cut(s) | 342 |
| FspBI | CTAG | 3 cut(s) | 21, 239, 690 |
| GluI | GCNGC | 1 cut(s) | 342 |
| GsaI | CCCAGC | 1 cut(s) | 237 |
| GsuI | CTGGAG | 2 cut(s) | 267, 648 |
| HaeIII | GGCC | 1 cut(s) | 533 |
| HapII | CCGG | 1 cut(s) | 60 |
| Hin1II | CATG | 3 cut(s) | 31, 454, 514 |
| HincII | GTYRAC | 1 cut(s) | 366 |
| HindII | GTYRAC | 1 cut(s) | 366 |
| HindIII | AAGCTT | 1 cut(s) | 120 |
| HinfI | GANTC | 1 cut(s) | 394 |
| HpaII | CCGG | 1 cut(s) | 60 |
| HphI | GGTGA | 1 cut(s) | 335 |
| Hpy166II | GTNNAC | 2 cut(s) | 366, 586 |
| Hpy188I | TCNGA | 2 cut(s) | 376, 543 |
| Hpy8I | GTNNAC | 2 cut(s) | 366, 586 |
| HpyAV | CCTTC | 2 cut(s) | 530, 696 |
| HpyCH4III | ACNGT | 4 cut(s) | 151, 161, 206, 611 |
| HpyCH4IV | ACGT | 1 cut(s) | 86 |
| HpyCH4V | TGCA | 5 cut(s) | 356, 563, 620, 653, 712 |
| HpyF3I | CTNAG | 2 cut(s) | 316, 375 |
| HpySE526I | ACGT | 1 cut(s) | 86 |
| Hsp92II | CATG | 3 cut(s) | 31, 454, 514 |
| Kzo9I | GATC | 1 cut(s) | 127 |
| LmnI | GCTCC | 3 cut(s) | 230, 286, 629 |
| LpnPI | CCDG | 6 cut(s) | 73, 90, 219, 297, 541, 612 |
| MaeI | CTAG | 3 cut(s) | 21, 239, 690 |
| MaeII | ACGT | 1 cut(s) | 86 |
| MaeIII | GTNAC | 2 cut(s) | 217, 304 |
| MalI | GATC | 1 cut(s) | 129 |
| MboI | GATC | 1 cut(s) | 127 |
| MboII | GAAGA | 3 cut(s) | 262, 508, 692 |
| MfeI | CAATTG | 2 cut(s) | 621, 638 |
| MhlI | GDGCHC | 1 cut(s) | 9 |
| MlsI | TGGCCA | 1 cut(s) | 533 |
| MluCI | AATT | 6 cut(s) | 222, 326, 535, 621, 633, 638 |
| MluNI | TGGCCA | 1 cut(s) | 533 |
| MnlI | CCTC | 2 cut(s) | 411, 474 |
| Mox20I | TGGCCA | 1 cut(s) | 533 |
| MscI | TGGCCA | 1 cut(s) | 533 |
| MseI | TTAA | 2 cut(s) | 155, 225 |
| Msp20I | TGGCCA | 1 cut(s) | 533 |
| MspI | CCGG | 1 cut(s) | 60 |
| MspR9I | CCNGG | 1 cut(s) | 61 |
| MunI | CAATTG | 2 cut(s) | 621, 638 |
| Mva1269I | GAATGC | 1 cut(s) | 189 |
| NciI | CCSGG | 1 cut(s) | 61 |
| NdeI | CATATG | 1 cut(s) | 565 |
| NdeII | GATC | 1 cut(s) | 127 |
| NlaIII | CATG | 3 cut(s) | 31, 454, 514 |
| NmuCI | GTSAC | 1 cut(s) | 217 |
| NspI | RCATGY | 1 cut(s) | 514 |
| PctI | GAATGC | 1 cut(s) | 189 |
| PfeI | GAWTC | 1 cut(s) | 394 |
| PkrI | GCNGC | 1 cut(s) | 343 |
| PshBI | ATTAAT | 1 cut(s) | 225 |
| PspEI | GGTNACC | 1 cut(s) | 304 |
| PspFI | CCCAGC | 1 cut(s) | 233 |
| PstNI | CAGNNNCTG | 1 cut(s) | 17 |
| SaqAI | TTAA | 2 cut(s) | 155, 225 |
| SatI | GCNGC | 1 cut(s) | 342 |
| Sau3AI | GATC | 1 cut(s) | 127 |
| ScrFI | CCNGG | 1 cut(s) | 61 |
| SduI | GDGCHC | 1 cut(s) | 9 |
| SfcI | CTRYAG | 1 cut(s) | 147 |
| Sse9I | AATT | 6 cut(s) | 222, 326, 535, 621, 633, 638 |
| SsiI | CCGC | 2 cut(s) | 341, 406 |
| SspI | AATATT | 1 cut(s) | 657 |
| SspMI | CTAG | 3 cut(s) | 21, 239, 690 |
| StyD4I | CCNGG | 1 cut(s) | 59 |
| TaaI | ACNGT | 4 cut(s) | 151, 161, 206, 611 |
| TaiI | ACGT | 1 cut(s) | 89 |
| TaqI | TCGA | 1 cut(s) | 397 |
| TasI | AATT | 6 cut(s) | 222, 326, 535, 621, 633, 638 |
| TauI | GCSGC | 1 cut(s) | 344 |
| TfiI | GAWTC | 1 cut(s) | 394 |
| Tru1I | TTAA | 2 cut(s) | 155, 225 |
| Tru9I | TTAA | 2 cut(s) | 155, 225 |
| TscAI | CASTG | 1 cut(s) | 156 |
| TseFI | GTSAC | 1 cut(s) | 217 |
| Tsp45I | GTSAC | 1 cut(s) | 217 |
| TspDTI | ATGAA | 5 cut(s) | 16, 407, 460, 583, 720 |
| TspGWI | ACGGA | 2 cut(s) | 195, 310 |
| TspRI | CASTG | 1 cut(s) | 156 |
| VspI | ATTAAT | 1 cut(s) | 225 |
| XapI | RAATTY | 1 cut(s) | 326 |
| XceI | RCATGY | 1 cut(s) | 514 |
| XspI | CTAG | 3 cut(s) | 21, 239, 690 |