Mediates both low-affinity uptake and efflux of sugar across the membrane
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Ion_trans | PF00520 | 59 - 307 | 3.6e-44 | Ion transport protein |
| Ion_trans_2 | PF07885 | 220 - 300 | 1.1e-12 | Ion channel |
| cNMP_binding | PF00027 | 398 - 483 | 1.1e-15 | Cyclic nucleotide-binding domain |
| KHA | PF11834 | 688 - 757 | 3.2e-23 | KHA, dimerisation domain of potassium ion channel |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 290, 2240 |
| AccB1I | GGYRCC | 1 cut(s) | 522 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 110 |
| AciI | CCGC | 3 cut(s) | 219, 980, 1414 |
| AclWI | GGATC | 3 cut(s) | 701, 1248, 2054 |
| AcoI | YGGCCR | 1 cut(s) | 1690 |
| AcsI | RAATTY | 8 cut(s) | 22, 712, 956, 1318, 1420, 1701, 1938, 2290 |
| AcuI | CTGAAG | 2 cut(s) | 13, 936 |
| AcyI | GRCGYC | 1 cut(s) | 523 |
| AfaI | GTAC | 7 cut(s) | 398, 501, 630, 707, 758, 1193, 2297 |
| AfeI | AGCGCT | 1 cut(s) | 1077 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 110, 1568 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 352 |
| AjnI | CCWGG | 5 cut(s) | 221, 407, 1537, 1561, 1719 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1686, 1718 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 812, 844 |
| Alw21I | GWGCWC | 2 cut(s) | 542, 599 |
| Alw26I | GTCTC | 4 cut(s) | 789, 1046, 1261, 1818 |
| Alw44I | GTGCAC | 1 cut(s) | 595 |
| AlwI | GGATC | 3 cut(s) | 701, 1248, 2054 |
| Aor51HI | AGCGCT | 1 cut(s) | 1077 |
| AoxI | GGCC | 4 cut(s) | 1230, 1361, 1690, 1723 |
| ApaLI | GTGCAC | 1 cut(s) | 595 |
| ApeKI | GCWGC | 9 cut(s) | 78, 480, 947, 962, 1045, 1598, 1709, 2113, 2155 |
| ApoI | RAATTY | 8 cut(s) | 22, 712, 956, 1318, 1420, 1701, 1938, 2290 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1197, 1229 |
| AseI | ATTAAT | 1 cut(s) | 2126 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 26, 1215 |
| AspLEI | GCGC | 2 cut(s) | 525, 1078 |
| AspS9I | GGNCC | 3 cut(s) | 994, 1231, 1723 |
| AsuHPI | GGTGA | 4 cut(s) | 1088, 1349, 1694, 2267 |
| AvaII | GGWCC | 1 cut(s) | 994 |
| BaeGI | GKGCMC | 2 cut(s) | 599, 2015 |
| BalI | TGGCCA | 1 cut(s) | 1692 |
| BanI | GGYRCC | 1 cut(s) | 522 |
| BanII | GRGCYC | 1 cut(s) | 542 |
| BbsI | GAAGAC | 3 cut(s) | 1461, 1962, 2238 |
| Bbv12I | GWGCWC | 2 cut(s) | 542, 599 |
| BbvI | GCAGC | 9 cut(s) | 90, 492, 934, 974, 1057, 1610, 1696, 2125, 2142 |
| BccI | CCATC | 7 cut(s) | 112, 1107, 1283, 1499, 1734, 1785, 2246 |
| BciT130I | CCWGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| BciVI | GTATCC | 1 cut(s) | 1321 |
| BclI | TGATCA | 1 cut(s) | 2257 |
| BcoDI | GTCTC | 4 cut(s) | 789, 1046, 1261, 1818 |
| BfaI | CTAG | 4 cut(s) | 90, 1808, 2019, 2169 |
| BfmI | CTRYAG | 1 cut(s) | 2188 |
| BfoI | RGCGCY | 2 cut(s) | 526, 1079 |
| BfuI | GTATCC | 1 cut(s) | 1321 |
| BmcAI | AGTACT | 2 cut(s) | 501, 1193 |
| Bme1390I | CCNGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| Bme18I | GGWCC | 1 cut(s) | 994 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 352 |
| BmgT120I | GGNCC | 3 cut(s) | 994, 1231, 1723 |
| BmiI | GGNNCC | 3 cut(s) | 115, 524, 1233 |
| BmrFI | CCNGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| BmsI | GCATC | 7 cut(s) | 256, 813, 1174, 1562, 1585, 1785, 1919 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1557 |
| BpiI | GAAGAC | 3 cut(s) | 1461, 1962, 2238 |
| BpmI | CTGGAG | 1 cut(s) | 1584 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1510 |
| BpuEI | CTTGAG | 3 cut(s) | 578, 1004, 2002 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2256 |
| BsaHI | GRCGYC | 1 cut(s) | 523 |
| BsaI | GGTCTC | 2 cut(s) | 1046, 1261 |
| BsaJI | CCNNGG | 3 cut(s) | 815, 1138, 1720 |
| BsaWI | WCCGGW | 1 cut(s) | 1777 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 25, 55, 812, 842, 1752, 1782 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 110, 1568 |
| Bse1I | ACTGG | 4 cut(s) | 352, 764, 790, 1257 |
| Bse3DI | GCAATG | 3 cut(s) | 759, 1089, 1608 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2256 |
| BseBI | CCWGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| BseDI | CCNNGG | 3 cut(s) | 815, 1138, 1720 |
| BseGI | GGATG | 4 cut(s) | 1577, 1796, 1800, 1882 |
| BseJI | GATNNNNATC | 1 cut(s) | 2256 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 110, 1568 |
| BseMI | GCAATG | 3 cut(s) | 759, 1089, 1608 |
| BseMII | CTCAG | 6 cut(s) | 86, 541, 798, 1272, 1524, 2173 |
| BseNI | ACTGG | 4 cut(s) | 352, 764, 790, 1257 |
| BseRI | GAGGAG | 2 cut(s) | 1581, 1773 |
| BseSI | GKGCMC | 2 cut(s) | 599, 2015 |
| BseXI | GCAGC | 9 cut(s) | 90, 492, 934, 974, 1057, 1610, 1696, 2125, 2142 |
| BsgI | GTGCAG | 1 cut(s) | 720 |
| BshFI | GGCC | 4 cut(s) | 1232, 1363, 1692, 1725 |
| BshNI | GGYRCC | 1 cut(s) | 522 |
| BsiHKAI | GWGCWC | 2 cut(s) | 542, 599 |
| BsiSI | CCGG | 3 cut(s) | 12, 514, 1778 |
| BslFI | GGGAC | 1 cut(s) | 947 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 110, 1568 |
| BsmAI | GTCTC | 4 cut(s) | 789, 1046, 1261, 1818 |
| BsmFI | GGGAC | 1 cut(s) | 947 |
| BsmI | GAATGC | 4 cut(s) | 245, 2083, 2215, 2224 |
| BsnI | GGCC | 4 cut(s) | 1232, 1363, 1692, 1725 |
| Bso31I | GGTCTC | 2 cut(s) | 1046, 1261 |
| Bsp1286I | GDGCHC | 3 cut(s) | 542, 599, 2015 |
| Bsp143I | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| BspACI | CCGC | 3 cut(s) | 219, 980, 1414 |
| BspANI | GGCC | 4 cut(s) | 1232, 1363, 1692, 1725 |
| BspCNI | CTCAG | 6 cut(s) | 85, 540, 799, 1271, 1523, 2172 |
| BspHI | TCATGA | 1 cut(s) | 1470 |
| BspLI | GGNNCC | 3 cut(s) | 115, 524, 1233 |
| BspPI | GGATC | 3 cut(s) | 701, 1248, 2054 |
| BspT107I | GGYRCC | 1 cut(s) | 522 |
| BspTNI | GGTCTC | 2 cut(s) | 1046, 1261 |
| BsrDI | GCAATG | 3 cut(s) | 759, 1089, 1608 |
| BsrI | ACTGG | 4 cut(s) | 352, 764, 790, 1257 |
| BssECI | CCNNGG | 3 cut(s) | 815, 1138, 1720 |
| BssMI | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| BssNI | GRCGYC | 1 cut(s) | 523 |
| BssT1I | CCWWGG | 2 cut(s) | 815, 1138 |
| Bst2UI | CCWGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| Bst4CI | ACNGT | 3 cut(s) | 299, 940, 1378 |
| Bst6I | CTCTTC | 3 cut(s) | 1109, 1646, 1861 |
| BstACI | GRCGYC | 1 cut(s) | 523 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2219 |
| BstC8I | GCNNGC | 4 cut(s) | 804, 945, 1641, 2118 |
| BstDEI | CTNAG | 6 cut(s) | 72, 527, 807, 1258, 1510, 2159 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1566 |
| BstF5I | GGATG | 4 cut(s) | 1577, 1796, 1800, 1882 |
| BstH2I | RGCGCY | 2 cut(s) | 526, 1079 |
| BstHHI | GCGC | 2 cut(s) | 525, 1078 |
| BstKTI | GATC | 7 cut(s) | 97, 696, 1066, 1243, 2049, 2054, 2260 |
| BstMAI | GTCTC | 4 cut(s) | 789, 1046, 1261, 1818 |
| BstMBI | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 489, 1581, 1640, 2019, 2219 |
| BstNI | CCWGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| BstNSI | RCATGY | 4 cut(s) | 511, 806, 847, 2083 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1557 |
| BstSCI | CCNGG | 5 cut(s) | 221, 407, 1537, 1561, 1719 |
| BstSFI | CTRYAG | 1 cut(s) | 2188 |
| BstSLI | GKGCMC | 2 cut(s) | 599, 2015 |
| BstV1I | GCAGC | 9 cut(s) | 90, 492, 934, 974, 1057, 1610, 1696, 2125, 2142 |
| BstV2I | GAAGAC | 3 cut(s) | 1461, 1962, 2238 |
| BstX2I | RGATCY | 1 cut(s) | 1240 |
| BstYI | RGATCY | 1 cut(s) | 1240 |
| BsuI | GTATCC | 1 cut(s) | 1321 |
| BsuRI | GGCC | 4 cut(s) | 1232, 1363, 1692, 1725 |
| BtsCI | GGATG | 4 cut(s) | 1577, 1796, 1800, 1882 |
| BtsI | GCAGTG | 3 cut(s) | 432, 708, 747 |
| BtsIMutI | CAGTG | 6 cut(s) | 432, 708, 747, 783, 1424, 2015 |
| Cac8I | GCNNGC | 4 cut(s) | 804, 945, 1641, 2118 |
| CciI | TCATGA | 1 cut(s) | 1470 |
| CfoI | GCGC | 2 cut(s) | 525, 1078 |
| Cfr13I | GGNCC | 3 cut(s) | 994, 1231, 1723 |
| CsiI | ACCWGGT | 1 cut(s) | 407 |
| Csp6I | GTAC | 7 cut(s) | 397, 500, 629, 706, 757, 1192, 2296 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 879, 914 |
| CviQI | GTAC | 7 cut(s) | 397, 500, 629, 706, 757, 1192, 2296 |
| DdeI | CTNAG | 6 cut(s) | 72, 527, 807, 1258, 1510, 2159 |
| DinI | GGCGCC | 1 cut(s) | 524 |
| DpnI | GATC | 7 cut(s) | 96, 695, 1065, 1242, 2048, 2053, 2259 |
| DpnII | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| DraI | TTTAAA | 1 cut(s) | 496 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 290, 2240 |
| DriI | GACNNNNNGTC | 1 cut(s) | 352 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 290, 2240 |
| EaeI | YGGCCR | 1 cut(s) | 1690 |
| Eam1104I | CTCTTC | 3 cut(s) | 1109, 1646, 1861 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 352 |
| EarI | CTCTTC | 3 cut(s) | 1109, 1646, 1861 |
| EciI | GGCGGA | 1 cut(s) | 208 |
| Ecl136II | GAGCTC | 1 cut(s) | 540 |
| Eco130I | CCWWGG | 2 cut(s) | 815, 1138 |
| Eco24I | GRGCYC | 1 cut(s) | 542 |
| Eco31I | GGTCTC | 2 cut(s) | 1046, 1261 |
| Eco32I | GATATC | 1 cut(s) | 1132 |
| Eco47I | GGWCC | 1 cut(s) | 994 |
| Eco47III | AGCGCT | 1 cut(s) | 1077 |
| Eco53kI | GAGCTC | 1 cut(s) | 540 |
| Eco57I | CTGAAG | 2 cut(s) | 13, 936 |
| EcoICRI | GAGCTC | 1 cut(s) | 540 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1566 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1231 |
| EcoRII | CCWGG | 5 cut(s) | 221, 407, 1537, 1561, 1719 |
| EcoRV | GATATC | 1 cut(s) | 1132 |
| EcoT14I | CCWWGG | 2 cut(s) | 815, 1138 |
| EcoT22I | ATGCAT | 1 cut(s) | 2085 |
| EcoT38I | GRGCYC | 1 cut(s) | 542 |
| EgeI | GGCGCC | 1 cut(s) | 524 |
| EheI | GGCGCC | 1 cut(s) | 524 |
| ErhI | CCWWGG | 2 cut(s) | 815, 1138 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 11, 43, 255, 287 |
| FaqI | GGGAC | 1 cut(s) | 947 |
| FauNDI | CATATG | 2 cut(s) | 1013, 1150 |
| FbaI | TGATCA | 1 cut(s) | 2257 |
| FokI | GGATG | 4 cut(s) | 1584, 1803, 1807, 1889 |
| FriOI | GRGCYC | 1 cut(s) | 542 |
| FspBI | CTAG | 4 cut(s) | 90, 1808, 2019, 2169 |
| GlaI | GCGC | 2 cut(s) | 524, 1077 |
| GsuI | CTGGAG | 1 cut(s) | 1584 |
| HaeII | RGCGCY | 2 cut(s) | 526, 1079 |
| HaeIII | GGCC | 4 cut(s) | 1232, 1363, 1692, 1725 |
| HapII | CCGG | 3 cut(s) | 12, 514, 1778 |
| HhaI | GCGC | 2 cut(s) | 525, 1078 |
| Hin1I | GRCGYC | 1 cut(s) | 523 |
| Hin6I | GCGC | 2 cut(s) | 523, 1076 |
| HinP1I | GCGC | 2 cut(s) | 523, 1076 |
| HincII | GTYRAC | 3 cut(s) | 295, 370, 1771 |
| HindII | GTYRAC | 3 cut(s) | 295, 370, 1771 |
| HinfI | GANTC | 6 cut(s) | 120, 573, 589, 677, 1410, 2141 |
| HpaII | CCGG | 3 cut(s) | 12, 514, 1778 |
| HphI | GGTGA | 4 cut(s) | 1088, 1349, 1694, 2267 |
| Hpy166II | GTNNAC | 6 cut(s) | 295, 370, 468, 597, 706, 1771 |
| Hpy188III | TCNNGA | 9 cut(s) | 31, 250, 344, 586, 1027, 1471, 1730, 1914, 2206 |
| Hpy8I | GTNNAC | 6 cut(s) | 295, 370, 468, 597, 706, 1771 |
| HpyAV | CCTTC | 4 cut(s) | 556, 1028, 1748, 1928 |
| HpyCH4III | ACNGT | 3 cut(s) | 299, 940, 1378 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 489, 1581, 1640, 2019, 2219 |
| HpyF3I | CTNAG | 6 cut(s) | 72, 527, 807, 1258, 1510, 2159 |
| Hsp92I | GRCGYC | 1 cut(s) | 523 |
| HspAI | GCGC | 2 cut(s) | 523, 1076 |
| KasI | GGCGCC | 1 cut(s) | 522 |
| Ksp22I | TGATCA | 1 cut(s) | 2257 |
| Kzo9I | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| LmnI | GCTCC | 5 cut(s) | 113, 144, 516, 545, 1760 |
| Lsp1109I | GCAGC | 9 cut(s) | 90, 492, 934, 974, 1057, 1610, 1696, 2125, 2142 |
| LweI | GCATC | 7 cut(s) | 256, 813, 1174, 1562, 1585, 1785, 1919 |
| MabI | ACCWGGT | 1 cut(s) | 407 |
| MaeI | CTAG | 4 cut(s) | 90, 1808, 2019, 2169 |
| MaeIII | GTNAC | 7 cut(s) | 192, 613, 745, 1005, 2030, 2068, 2305 |
| MalI | GATC | 7 cut(s) | 96, 695, 1065, 1242, 2048, 2053, 2259 |
| MboI | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| MfeI | CAATTG | 4 cut(s) | 1166, 1555, 1621, 1927 |
| MflI | RGATCY | 1 cut(s) | 1240 |
| MhlI | GDGCHC | 3 cut(s) | 542, 599, 2015 |
| MlsI | TGGCCA | 1 cut(s) | 1692 |
| MluNI | TGGCCA | 1 cut(s) | 1692 |
| Mly113I | GGCGCC | 1 cut(s) | 523 |
| MlyI | GAGTC | 2 cut(s) | 583, 1404 |
| MmeI | TCCRAC | 1 cut(s) | 2085 |
| Mox20I | TGGCCA | 1 cut(s) | 1692 |
| Mph1103I | ATGCAT | 1 cut(s) | 2085 |
| MroXI | GAANNNNTTC | 2 cut(s) | 26, 1215 |
| MscI | TGGCCA | 1 cut(s) | 1692 |
| MseI | TTAA | 5 cut(s) | 495, 1274, 1544, 1850, 2126 |
| MslI | CAYNNNNRTG | 6 cut(s) | 611, 752, 1012, 1484, 1593, 2008 |
| Msp20I | TGGCCA | 1 cut(s) | 1692 |
| MspI | CCGG | 3 cut(s) | 12, 514, 1778 |
| MspR9I | CCNGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| MunI | CAATTG | 4 cut(s) | 1166, 1555, 1621, 1927 |
| Mva1269I | GAATGC | 4 cut(s) | 245, 2083, 2215, 2224 |
| MvaI | CCWGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 489, 1581, 1640, 2019, 2219 |
| NarI | GGCGCC | 1 cut(s) | 523 |
| NdeI | CATATG | 2 cut(s) | 1013, 1150 |
| NdeII | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| NlaIV | GGNNCC | 3 cut(s) | 115, 524, 1233 |
| NmeAIII | GCCGAG | 1 cut(s) | 1213 |
| NmuCI | GTSAC | 5 cut(s) | 613, 745, 1005, 2030, 2305 |
| NsiI | ATGCAT | 1 cut(s) | 2085 |
| NspI | RCATGY | 4 cut(s) | 511, 806, 847, 2083 |
| PaeI | GCATGC | 1 cut(s) | 806 |
| PagI | TCATGA | 1 cut(s) | 1470 |
| PctI | GAATGC | 4 cut(s) | 245, 2083, 2215, 2224 |
| PdmI | GAANNNNTTC | 2 cut(s) | 26, 1215 |
| PfeI | GAWTC | 4 cut(s) | 120, 573, 677, 2141 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 110 |
| PfoI | TCCNGGA | 1 cut(s) | 1561 |
| PleI | GAGTC | 2 cut(s) | 583, 1404 |
| PluTI | GGCGCC | 1 cut(s) | 526 |
| PpsI | GAGTC | 2 cut(s) | 583, 1404 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1557 |
| PshBI | ATTAAT | 1 cut(s) | 2126 |
| Psp124BI | GAGCTC | 1 cut(s) | 542 |
| Psp6I | CCWGG | 5 cut(s) | 221, 407, 1537, 1561, 1719 |
| PspGI | CCWGG | 5 cut(s) | 221, 407, 1537, 1561, 1719 |
| PspN4I | GGNNCC | 3 cut(s) | 115, 524, 1233 |
| PspPI | GGNCC | 3 cut(s) | 994, 1231, 1723 |
| PsuI | RGATCY | 1 cut(s) | 1240 |
| RsaI | GTAC | 7 cut(s) | 398, 501, 630, 707, 758, 1193, 2297 |
| RsaNI | GTAC | 7 cut(s) | 397, 500, 629, 706, 757, 1192, 2296 |
| RseI | CAYNNNNRTG | 6 cut(s) | 611, 752, 1012, 1484, 1593, 2008 |
| SacI | GAGCTC | 1 cut(s) | 542 |
| SaqAI | TTAA | 5 cut(s) | 495, 1274, 1544, 1850, 2126 |
| Sau3AI | GATC | 7 cut(s) | 94, 693, 1063, 1240, 2046, 2051, 2257 |
| Sau96I | GGNCC | 3 cut(s) | 994, 1231, 1723 |
| ScaI | AGTACT | 2 cut(s) | 501, 1193 |
| SchI | GAGTC | 2 cut(s) | 583, 1404 |
| ScrFI | CCNGG | 5 cut(s) | 223, 409, 1539, 1563, 1721 |
| SduI | GDGCHC | 3 cut(s) | 542, 599, 2015 |
| SexAI | ACCWGGT | 1 cut(s) | 407 |
| SfaNI | GCATC | 7 cut(s) | 256, 813, 1174, 1562, 1585, 1785, 1919 |
| SfcI | CTRYAG | 1 cut(s) | 2188 |
| SfoI | GGCGCC | 1 cut(s) | 524 |
| SinI | GGWCC | 1 cut(s) | 994 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 611, 752, 1012, 1484, 1593, 2008 |
| SmlI | CTYRAG | 3 cut(s) | 557, 1019, 1981 |
| SmoI | CTYRAG | 3 cut(s) | 557, 1019, 1981 |
| SphI | GCATGC | 1 cut(s) | 806 |
| SsiI | CCGC | 3 cut(s) | 219, 980, 1414 |
| SspDI | GGCGCC | 1 cut(s) | 522 |
| SspI | AATATT | 2 cut(s) | 1285, 1404 |
| SspMI | CTAG | 4 cut(s) | 90, 1808, 2019, 2169 |
| SstI | GAGCTC | 1 cut(s) | 542 |
| StyD4I | CCNGG | 5 cut(s) | 221, 407, 1537, 1561, 1719 |
| StyI | CCWWGG | 2 cut(s) | 815, 1138 |
| TaaI | ACNGT | 3 cut(s) | 299, 940, 1378 |
| TaqI | TCGA | 8 cut(s) | 237, 282, 345, 420, 571, 831, 908, 1128 |
| TatI | WGTACW | 5 cut(s) | 499, 628, 756, 1191, 2295 |
| TauI | GCSGC | 1 cut(s) | 982 |
| TfiI | GAWTC | 4 cut(s) | 120, 573, 677, 2141 |
| Tru1I | TTAA | 5 cut(s) | 495, 1274, 1544, 1850, 2126 |
| Tru9I | TTAA | 5 cut(s) | 495, 1274, 1544, 1850, 2126 |
| TscAI | CASTG | 6 cut(s) | 439, 708, 754, 790, 1431, 2015 |
| TseFI | GTSAC | 5 cut(s) | 613, 745, 1005, 2030, 2305 |
| TseI | GCWGC | 9 cut(s) | 78, 480, 947, 962, 1045, 1598, 1709, 2113, 2155 |
| Tsp45I | GTSAC | 5 cut(s) | 613, 745, 1005, 2030, 2305 |
| TspGWI | ACGGA | 1 cut(s) | 1253 |
| TspRI | CASTG | 6 cut(s) | 439, 708, 754, 790, 1431, 2015 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 110 |
| VneI | GTGCAC | 1 cut(s) | 595 |
| VpaK11BI | GGWCC | 1 cut(s) | 994 |
| VspI | ATTAAT | 1 cut(s) | 2126 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1566 |
| XapI | RAATTY | 8 cut(s) | 22, 712, 956, 1318, 1420, 1701, 1938, 2290 |
| XceI | RCATGY | 4 cut(s) | 511, 806, 847, 2083 |
| XmnI | GAANNNNTTC | 2 cut(s) | 26, 1215 |
| XspI | CTAG | 4 cut(s) | 90, 1808, 2019, 2169 |
| ZrmI | AGTACT | 2 cut(s) | 501, 1193 |
| Zsp2I | ATGCAT | 1 cut(s) | 2085 |