Mediates both low-affinity uptake and efflux of sugar across the membrane
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 279 |
| AclWI | GGATC | 1 cut(s) | 119 |
| AfaI | GTAC | 1 cut(s) | 706 |
| AgsI | TTSAA | 4 cut(s) | 154, 439, 469, 635 |
| AluBI | AGCT | 3 cut(s) | 119, 247, 700 |
| AluI | AGCT | 3 cut(s) | 119, 247, 700 |
| Alw21I | GWGCWC | 3 cut(s) | 9, 249, 631 |
| Alw26I | GTCTC | 2 cut(s) | 614, 674 |
| Alw44I | GTGCAC | 1 cut(s) | 5 |
| AlwI | GGATC | 1 cut(s) | 119 |
| AoxI | GGCC | 1 cut(s) | 58 |
| ApaLI | GTGCAC | 1 cut(s) | 5 |
| ApeKI | GCWGC | 2 cut(s) | 281, 697 |
| AseI | ATTAAT | 1 cut(s) | 222 |
| AsuHPI | GGTGA | 1 cut(s) | 397 |
| BaeGI | GKGCMC | 1 cut(s) | 9 |
| BanII | GRGCYC | 2 cut(s) | 249, 343 |
| Bbv12I | GWGCWC | 3 cut(s) | 9, 249, 631 |
| BbvI | GCAGC | 2 cut(s) | 293, 709 |
| BccI | CCATC | 1 cut(s) | 376 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 592, 626 |
| BclI | TGATCA | 1 cut(s) | 373 |
| BcoDI | GTCTC | 2 cut(s) | 614, 674 |
| BfaI | CTAG | 1 cut(s) | 18 |
| BisI | GCNGC | 3 cut(s) | 279, 282, 698 |
| BlsI | GCNGC | 3 cut(s) | 280, 283, 699 |
| BmsI | GCATC | 1 cut(s) | 4 |
| BsaI | GGTCTC | 1 cut(s) | 614 |
| BseGI | GGATG | 1 cut(s) | 557 |
| BseMII | CTCAG | 1 cut(s) | 464 |
| BseSI | GKGCMC | 1 cut(s) | 9 |
| BseXI | GCAGC | 2 cut(s) | 293, 709 |
| BsgI | GTGCAG | 1 cut(s) | 716 |
| BshFI | GGCC | 1 cut(s) | 60 |
| BsiHKAI | GWGCWC | 3 cut(s) | 9, 249, 631 |
| BsmAI | GTCTC | 2 cut(s) | 614, 674 |
| BsnI | GGCC | 1 cut(s) | 60 |
| Bso31I | GGTCTC | 1 cut(s) | 614 |
| Bsp1286I | GDGCHC | 4 cut(s) | 9, 249, 343, 631 |
| Bsp143I | GATC | 3 cut(s) | 48, 124, 373 |
| BspACI | CCGC | 1 cut(s) | 279 |
| BspANI | GGCC | 1 cut(s) | 60 |
| BspCNI | CTCAG | 1 cut(s) | 465 |
| BspPI | GGATC | 1 cut(s) | 119 |
| BspTNI | GGTCTC | 1 cut(s) | 614 |
| BssMI | GATC | 3 cut(s) | 48, 124, 373 |
| Bst4CI | ACNGT | 3 cut(s) | 148, 158, 608 |
| BstDEI | CTNAG | 3 cut(s) | 473, 492, 523 |
| BstF5I | GGATG | 1 cut(s) | 557 |
| BstKTI | GATC | 3 cut(s) | 51, 127, 376 |
| BstMAI | GTCTC | 2 cut(s) | 614, 674 |
| BstMBI | GATC | 3 cut(s) | 48, 124, 373 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 347 |
| BstSLI | GKGCMC | 1 cut(s) | 9 |
| BstV1I | GCAGC | 2 cut(s) | 293, 709 |
| BsuRI | GGCC | 1 cut(s) | 60 |
| BtsCI | GGATG | 1 cut(s) | 557 |
| BtsIMutI | CAGTG | 1 cut(s) | 153 |
| Csp6I | GTAC | 1 cut(s) | 705 |
| CviJI | RGCY | 9 cut(s) | 60, 119, 235, 247, 341, 350, 407, 456, 700 |
| CviKI_1 | RGCY | 9 cut(s) | 60, 119, 235, 247, 341, 350, 407, 456, 700 |
| CviQI | GTAC | 1 cut(s) | 705 |
| DdeI | CTNAG | 3 cut(s) | 473, 492, 523 |
| DpnI | GATC | 3 cut(s) | 50, 126, 375 |
| DpnII | GATC | 3 cut(s) | 48, 124, 373 |
| Ecl136II | GAGCTC | 1 cut(s) | 247 |
| Eco24I | GRGCYC | 2 cut(s) | 249, 343 |
| Eco31I | GGTCTC | 1 cut(s) | 614 |
| Eco53kI | GAGCTC | 1 cut(s) | 247 |
| EcoICRI | GAGCTC | 1 cut(s) | 247 |
| EcoT38I | GRGCYC | 2 cut(s) | 249, 343 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 684, 716 |
| FbaI | TGATCA | 1 cut(s) | 373 |
| Fnu4HI | GCNGC | 3 cut(s) | 279, 282, 698 |
| FokI | GGATG | 1 cut(s) | 544 |
| FriOI | GRGCYC | 2 cut(s) | 249, 343 |
| Fsp4HI | GCNGC | 3 cut(s) | 279, 282, 698 |
| FspBI | CTAG | 1 cut(s) | 18 |
| GluI | GCNGC | 3 cut(s) | 279, 282, 698 |
| HaeIII | GGCC | 1 cut(s) | 60 |
| HindIII | AAGCTT | 1 cut(s) | 117 |
| HinfI | GANTC | 1 cut(s) | 391 |
| HphI | GGTGA | 1 cut(s) | 397 |
| Hpy166II | GTNNAC | 2 cut(s) | 7, 583 |
| Hpy188III | TCNNGA | 1 cut(s) | 538 |
| Hpy8I | GTNNAC | 2 cut(s) | 7, 583 |
| HpyCH4III | ACNGT | 3 cut(s) | 148, 158, 608 |
| HpyCH4IV | ACGT | 1 cut(s) | 45 |
| HpyCH4V | TGCA | 4 cut(s) | 7, 617, 650, 697 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 347 |
| HpyF3I | CTNAG | 3 cut(s) | 473, 492, 523 |
| HpySE526I | ACGT | 1 cut(s) | 45 |
| Ksp22I | TGATCA | 1 cut(s) | 373 |
| Kzo9I | GATC | 3 cut(s) | 48, 124, 373 |
| LmnI | GCTCC | 1 cut(s) | 626 |
| LpnPI | CCDG | 7 cut(s) | 39, 151, 329, 384, 485, 538, 551 |
| Lsp1109I | GCAGC | 2 cut(s) | 293, 709 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 1 cut(s) | 18 |
| MaeII | ACGT | 1 cut(s) | 45 |
| MaeIII | GTNAC | 3 cut(s) | 170, 214, 304 |
| MalI | GATC | 3 cut(s) | 50, 126, 375 |
| MboI | GATC | 3 cut(s) | 48, 124, 373 |
| MboII | GAAGA | 1 cut(s) | 259 |
| MfeI | CAATTG | 1 cut(s) | 635 |
| MhlI | GDGCHC | 4 cut(s) | 9, 249, 343, 631 |
| MluCI | AATT | 4 cut(s) | 75, 228, 618, 635 |
| MmeI | TCCRAC | 1 cut(s) | 19 |
| MnlI | CCTC | 3 cut(s) | 77, 291, 396 |
| MseI | TTAA | 3 cut(s) | 192, 222, 486 |
| MslI | CAYNNNNRTG | 1 cut(s) | 12 |
| MunI | CAATTG | 1 cut(s) | 635 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 347 |
| NdeII | GATC | 3 cut(s) | 48, 124, 373 |
| NmuCI | GTSAC | 1 cut(s) | 214 |
| PfeI | GAWTC | 1 cut(s) | 391 |
| PkrI | GCNGC | 3 cut(s) | 280, 283, 699 |
| PshBI | ATTAAT | 1 cut(s) | 222 |
| Psp124BI | GAGCTC | 1 cut(s) | 249 |
| RsaI | GTAC | 1 cut(s) | 706 |
| RsaNI | GTAC | 1 cut(s) | 705 |
| RseI | CAYNNNNRTG | 1 cut(s) | 12 |
| SacI | GAGCTC | 1 cut(s) | 249 |
| SaqAI | TTAA | 3 cut(s) | 192, 222, 486 |
| SatI | GCNGC | 3 cut(s) | 279, 282, 698 |
| Sau3AI | GATC | 3 cut(s) | 48, 124, 373 |
| SduI | GDGCHC | 4 cut(s) | 9, 249, 343, 631 |
| SetI | ASST | 9 cut(s) | 23, 48, 121, 136, 141, 249, 373, 474, 702 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 12 |
| Sse9I | AATT | 4 cut(s) | 75, 228, 618, 635 |
| SsiI | CCGC | 1 cut(s) | 279 |
| SspMI | CTAG | 1 cut(s) | 18 |
| SstI | GAGCTC | 1 cut(s) | 249 |
| TaaI | ACNGT | 3 cut(s) | 148, 158, 608 |
| TaiI | ACGT | 1 cut(s) | 48 |
| TaqI | TCGA | 2 cut(s) | 123, 394 |
| TasI | AATT | 4 cut(s) | 75, 228, 618, 635 |
| TatI | WGTACW | 1 cut(s) | 704 |
| TauI | GCSGC | 1 cut(s) | 281 |
| TfiI | GAWTC | 1 cut(s) | 391 |
| Tru1I | TTAA | 3 cut(s) | 192, 222, 486 |
| Tru9I | TTAA | 3 cut(s) | 192, 222, 486 |
| TscAI | CASTG | 1 cut(s) | 153 |
| TseFI | GTSAC | 1 cut(s) | 214 |
| TseI | GCWGC | 2 cut(s) | 281, 697 |
| Tsp45I | GTSAC | 1 cut(s) | 214 |
| TspDTI | ATGAA | 4 cut(s) | 76, 404, 580, 705 |
| TspGWI | ACGGA | 1 cut(s) | 192 |
| TspRI | CASTG | 1 cut(s) | 153 |
| VneI | GTGCAC | 1 cut(s) | 5 |
| VspI | ATTAAT | 1 cut(s) | 222 |
| XspI | CTAG | 1 cut(s) | 18 |